CA2348779A1 - Detecteurs moleculaires polynucleotidiques a domaines multiples - Google Patents

Detecteurs moleculaires polynucleotidiques a domaines multiples Download PDF

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Publication number
CA2348779A1
CA2348779A1 CA002348779A CA2348779A CA2348779A1 CA 2348779 A1 CA2348779 A1 CA 2348779A1 CA 002348779 A CA002348779 A CA 002348779A CA 2348779 A CA2348779 A CA 2348779A CA 2348779 A1 CA2348779 A1 CA 2348779A1
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CA
Canada
Prior art keywords
domain
rna
allosteric
ligand
ribozyme
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002348779A
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English (en)
Inventor
Ronald R. Breaker
Garrett A. Soukup
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Yale University
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2348779A1 publication Critical patent/CA2348779A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1048—SELEX
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
    • C12N2310/121—Hammerhead
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/18—Type of nucleic acid acting by a non-sequence specific mechanism

Abstract

L'invention concerne des polynucléotides à domaines multiples, sensibles aux agents de signalisations, configurés et construits de manière à comprendre au moins trois domaines pouvant se chevaucher partiellement, entièrement ou pas du tout, à savoir un domaine activateur (catalytique ou rapporteur), un domaine de pontage et un domaine récepteur. Dans une forme d'exécution caractéristique, un agent de signalisation tel qu'un ligand chimique interagit avec le domaine récepteur, ce qui modifie ou influence d'une autre manière le domaine de pontage de telle façon que la fonction d'activation, catalytique ou rapporteuse du domaine d'actionnement est stimulée ou inhibée. Dans certaines formes à ribozymes par exemple, des détecteurs moléculaires spécifiques du ligand, composés d'ARN, sont créés par couplage du domaine catalytique et du domaine récepteur préexistants au moyen de nouveaux ponts structurels fonctionnant de la manière suivante : la fixation d'un ligand sur le domaine récepteur provoque une modification de la configuration du pont et cette réorganisation structurelle détermine l'activité du ribozyme contigu. L'invention concerne également des procédés permettant une sélection allostérique d'autres polynucléotides à domaines multiples, ces procédés consistant normalement à répartir et à coupler les domaines de manière à favoriser la liaison ou d'autres signaux réponse et/ou l'activité rapporteuse.
CA002348779A 1998-11-03 1999-10-29 Detecteurs moleculaires polynucleotidiques a domaines multiples Abandoned CA2348779A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US10682998P 1998-11-03 1998-11-03
US60/106,829 1998-11-03
US12668399P 1999-03-29 1999-03-29
US60/126,683 1999-03-29
PCT/US1999/025497 WO2000026226A1 (fr) 1998-11-03 1999-10-29 Detecteurs moleculaires polynucleotidiques a domaines multiples

Publications (1)

Publication Number Publication Date
CA2348779A1 true CA2348779A1 (fr) 2000-05-11

Family

ID=26804069

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002348779A Abandoned CA2348779A1 (fr) 1998-11-03 1999-10-29 Detecteurs moleculaires polynucleotidiques a domaines multiples

Country Status (5)

Country Link
EP (1) EP1133513A4 (fr)
JP (1) JP2002528109A (fr)
AU (2) AU772881B2 (fr)
CA (1) CA2348779A1 (fr)
WO (1) WO2000026226A1 (fr)

Families Citing this family (62)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7034009B2 (en) 1995-10-26 2006-04-25 Sirna Therapeutics, Inc. Enzymatic nucleic acid-mediated treatment of ocular diseases or conditions related to levels of vascular endothelial growth factor receptor (VEGF-R)
US6482932B1 (en) 1997-11-05 2002-11-19 Ribozyme Pharmaceuticals, Incorporated Nucleoside triphosphates and their incorporation into oligonucleotides
US6617438B1 (en) 1997-11-05 2003-09-09 Sirna Therapeutics, Inc. Oligoribonucleotides with enzymatic activity
US6995259B1 (en) 1998-10-23 2006-02-07 Sirna Therapeutics, Inc. Method for the chemical synthesis of oligonucleotides
IL130606A0 (en) * 1999-06-23 2000-06-01 Intelligene Ltd An array of nucleic acid sequence
US6506887B1 (en) 1999-07-29 2003-01-14 Somalogic, Incorporated Conditional-selex
US7491805B2 (en) 2001-05-18 2009-02-17 Sirna Therapeutics, Inc. Conjugates and compositions for cellular delivery
US7833992B2 (en) 2001-05-18 2010-11-16 Merck Sharpe & Dohme Conjugates and compositions for cellular delivery
WO2002081628A2 (fr) 2001-04-05 2002-10-17 Ribozyme Pharmaceuticals, Incorporated Modulation de l'expression genique associee a la proliferation inflammatoire et a la croissance de neurites, par des procedes faisant intervenir l'acide nucleique
AU2001266917A1 (en) * 2000-06-15 2001-12-24 Board Of Regents Allosterically regulated ribozymes
US20030104520A1 (en) * 2000-06-15 2003-06-05 Ellington Andrew D. Regulatable, catalytically active nucleic acids
US6706474B1 (en) 2000-06-27 2004-03-16 Board Of Trustees Of The University Of Illinois Nucleic acid enzyme biosensors for ions
CA2440367A1 (fr) * 2000-10-20 2002-08-15 Canji, Inc. Regulation de l'expression genique au moyen d'aptameres
US9994853B2 (en) 2001-05-18 2018-06-12 Sirna Therapeutics, Inc. Chemically modified multifunctional short interfering nucleic acid molecules that mediate RNA interference
EP1572067A4 (fr) 2001-05-18 2009-05-13 Sirna Therapeutics Inc Conjugues et compositions pour administration cellulaire
US7109165B2 (en) 2001-05-18 2006-09-19 Sirna Therapeutics, Inc. Conjugates and compositions for cellular delivery
US7205399B1 (en) 2001-07-06 2007-04-17 Sirna Therapeutics, Inc. Methods and reagents for oligonucleotide synthesis
US20030219422A1 (en) * 2001-11-15 2003-11-27 Noxxon Pharma Ag Allosteric ribozymes and uses thereof
AU2002357240A1 (en) * 2001-12-14 2003-06-30 Yale University Nucleic acid diagnostic reagents and methods for detecting nucleic acids, polynucleotides and oligonucleotides
US7071311B2 (en) 2002-02-13 2006-07-04 Sirna Therapeutics, Inc. Antibodies having specificity for 2′-C-allyl nucleic acids
US9181551B2 (en) 2002-02-20 2015-11-10 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US9657294B2 (en) 2002-02-20 2017-05-23 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US6890719B2 (en) 2002-05-10 2005-05-10 The Board Of Trustess Of The University Of Illinois Fluorescence based biosensor
US7534560B2 (en) 2002-05-10 2009-05-19 The Board Of Trustees Of The University Of Illinois Simple catalytic DNA biosensors for ions based on color changes
US20040110235A1 (en) 2002-07-25 2004-06-10 David Epstein Regulated aptamer therapeutics
EP2322535A3 (fr) * 2002-09-20 2011-09-28 Yale University Riboswitchs, procedes d'utilisation et compositions a utiliser avec des riboswitchs
US7612185B2 (en) 2003-03-07 2009-11-03 The Board Of Trustees Of The University Of Illinois Nucleic acid biosensors
EP1622572B1 (fr) 2003-04-30 2017-12-20 Sirna Therapeutics, Inc. Conjugues et compositions pour la livraison cellulaire
US7485419B2 (en) 2004-01-13 2009-02-03 The Board Of Trustees Of The University Of Illinois Biosensors based on directed assembly of particles
US10508277B2 (en) 2004-05-24 2019-12-17 Sirna Therapeutics, Inc. Chemically modified multifunctional short interfering nucleic acid molecules that mediate RNA interference
US7892734B2 (en) 2005-08-11 2011-02-22 The Board Of Trustees Of The University Of Illinois Aptamer based colorimetric sensor systems
US8313901B2 (en) * 2005-12-21 2012-11-20 Yale University Methods and compositions related to the modulation of riboswitches
US7799554B2 (en) 2006-03-16 2010-09-21 The Board Of Trustees Of The University Of Illinois Lateral flow devices
EP2471809B1 (fr) 2006-07-11 2015-09-02 University Of Medicine And Dentistry Of New Jersey Protéines, acides nucléiques codant pour celles-ci et procédés d'utilisation correspondants
WO2008089248A2 (fr) 2007-01-19 2008-07-24 The Board Of Trustees Of The University Of Illinois Substances amphiphiles et vésicules lipidiques fonctionnalisées contenant de telles substances
US8058415B2 (en) 2007-04-24 2011-11-15 The Board Of Trustees Of The University Of Illinois Aptamer- and nucleic acid enzyme-based systems for simultaneous detection of multiple analytes
US8409800B2 (en) 2007-07-16 2013-04-02 The Board Of Trustees Of The University Of Illinois Nucleic acid based fluorescent sensor for copper detection
US8568690B2 (en) 2007-07-31 2013-10-29 The Board Of Trustees Of The University Of Illinois MRI contrast agents and high-throughput screening by MRI
WO2009045632A2 (fr) 2007-08-10 2009-04-09 The Board Of Trustees Of The University Of Illinois Capteur fluorescent à base d'acide nucléique destiné à la détection du mercure
US8062893B2 (en) 2008-10-10 2011-11-22 The Board Of Trustees Of The University Of Illinois Fluorescent sensor for mercury
SG181904A1 (en) 2009-12-23 2012-07-30 Novartis Ag Lipids, lipid compositions, and methods of using them
EP2600901B1 (fr) 2010-08-06 2019-03-27 ModernaTX, Inc. Compositions pharmaceutiques a base d'acides nucléiques modifiés et leur utilisation medicale
ES2737960T3 (es) 2010-10-01 2020-01-17 Modernatx Inc Nucleósidos, nucleótidos y ácidos nucleicos modificados y sus usos
EP3766975A1 (fr) 2010-10-29 2021-01-20 Sirna Therapeutics, Inc. Inhibition au moyen d'interférence arn d'une expression de gène utilisant des acides nucléiques à petit interférent (sina)
AU2012236099A1 (en) 2011-03-31 2013-10-03 Moderna Therapeutics, Inc. Delivery and formulation of engineered nucleic acids
US9464124B2 (en) 2011-09-12 2016-10-11 Moderna Therapeutics, Inc. Engineered nucleic acids and methods of use thereof
EP3682905B1 (fr) 2011-10-03 2021-12-01 ModernaTX, Inc. Nucléosides, nucléotides et acides nucléiques modifiés et leurs utilisations
EP2791160B1 (fr) 2011-12-16 2022-03-02 ModernaTX, Inc. Compositions de mrna modifiés
US9283287B2 (en) 2012-04-02 2016-03-15 Moderna Therapeutics, Inc. Modified polynucleotides for the production of nuclear proteins
US9572897B2 (en) 2012-04-02 2017-02-21 Modernatx, Inc. Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins
US9303079B2 (en) 2012-04-02 2016-04-05 Moderna Therapeutics, Inc. Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins
AU2013243948A1 (en) 2012-04-02 2014-10-30 Moderna Therapeutics, Inc. Modified polynucleotides for the production of proteins associated with human disease
JP5731016B2 (ja) * 2012-04-27 2015-06-10 キリン株式会社 リガンドを高感度に検出する核酸分子並びに該核酸分子のスクリーニング方法および該核酸分子の感度の最適化方法
EP4074834A1 (fr) 2012-11-26 2022-10-19 ModernaTX, Inc. Arn à terminaison modifiée
BR112015021791B1 (pt) 2013-03-08 2022-08-30 Novartis Ag Compostos de lipídio catiônico e composições de lipídios e farmacêuticas
US8980864B2 (en) 2013-03-15 2015-03-17 Moderna Therapeutics, Inc. Compositions and methods of altering cholesterol levels
WO2015048744A2 (fr) 2013-09-30 2015-04-02 Moderna Therapeutics, Inc. Polynucléotides codant des polypeptides de modulation immunitaire
CA2926218A1 (fr) 2013-10-03 2015-04-09 Moderna Therapeutics, Inc. Polynucleotides codant pour un recepteur de lipoproteines de faible densite
EP4019506A1 (fr) 2013-12-19 2022-06-29 Novartis AG Lipides et compositions lipidiques pour l'administration d'agents actifs
PL3083556T3 (pl) 2013-12-19 2020-06-29 Novartis Ag Lipidy i kompozycje lipidowe dla dostarczania środków czynnych
EP4223285A3 (fr) 2014-07-16 2023-11-22 Novartis AG Procédé d'encapsulation d'un acide nucléique dans un hôte de nanoparticules lipidiques
EP3188760B1 (fr) 2014-09-05 2023-12-06 Novartis AG Lipides et compositions lipidiques permettant l'administration de principes actifs

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU724627B2 (en) * 1996-12-19 2000-09-28 Yale University Bioreactive allosteric polynucleotides

Also Published As

Publication number Publication date
EP1133513A4 (fr) 2002-07-03
WO2000026226A1 (fr) 2000-05-11
EP1133513A1 (fr) 2001-09-19
AU1601300A (en) 2000-05-22
JP2002528109A (ja) 2002-09-03
AU2004203816A1 (en) 2004-09-02
AU772881B2 (en) 2004-05-13

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued