CA2324344A1 - Methode de detection par fluorescence d'inhibiteurs de topoisomerase - Google Patents

Methode de detection par fluorescence d'inhibiteurs de topoisomerase Download PDF

Info

Publication number
CA2324344A1
CA2324344A1 CA002324344A CA2324344A CA2324344A1 CA 2324344 A1 CA2324344 A1 CA 2324344A1 CA 002324344 A CA002324344 A CA 002324344A CA 2324344 A CA2324344 A CA 2324344A CA 2324344 A1 CA2324344 A1 CA 2324344A1
Authority
CA
Canada
Prior art keywords
dna
fluid
topoisomerase
test compound
gyrase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002324344A
Other languages
English (en)
Inventor
Carl Edward Catrenich
Kelly Maureen Makin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Procter and Gamble Co
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2324344A1 publication Critical patent/CA2324344A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/533—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving isomerase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/99—Isomerases (5.)

Abstract

L'invention concerne des procédés servant à déterminer l'activité de composés d'essai en tant qu'inhibiteurs de topoisomérase et consistant à mélanger, dans un liquide, un composé d'essai, un A D N et une topoisomérase; à incuber le liquide, à incorporer un colorant d'acide nucléique à base de cyanine, tel que PicoGreen?, à mesurer la fluorescence et à comparer cette dernière à la fluorescence émise par des témoins.
CA002324344A 1998-03-25 1999-03-22 Methode de detection par fluorescence d'inhibiteurs de topoisomerase Abandoned CA2324344A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US7926598P 1998-03-25 1998-03-25
US60/079,265 1998-03-25
PCT/IB1999/000473 WO1999049077A1 (fr) 1998-03-25 1999-03-22 Methode de detection par fluorescence d'inhibiteurs de topoisomerase

Publications (1)

Publication Number Publication Date
CA2324344A1 true CA2324344A1 (fr) 1999-09-30

Family

ID=22149463

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002324344A Abandoned CA2324344A1 (fr) 1998-03-25 1999-03-22 Methode de detection par fluorescence d'inhibiteurs de topoisomerase

Country Status (7)

Country Link
EP (1) EP1086242A1 (fr)
JP (1) JP2002507433A (fr)
AU (1) AU3269999A (fr)
CA (1) CA2324344A1 (fr)
IL (1) IL138532A0 (fr)
NO (1) NO20004753L (fr)
WO (1) WO1999049077A1 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2001270098A1 (en) * 2000-06-22 2002-01-02 San Diego State University Foundation Recombination modulators and methods for their production and use
GB0424953D0 (en) * 2004-11-11 2004-12-15 Plant Bioscience Ltd Assay
WO2010044101A1 (fr) * 2008-10-08 2010-04-22 V. B. Medicare Pvt. Ltd. Purification et dosage de topoisomérases et utilisation du dosage pour le criblage de modulateurs de topoisomérases
US20140106342A1 (en) * 2011-02-10 2014-04-17 Mark T. Muller Method of assaying dna topoisomerases and dna binding proteins using high throughput screening

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992020813A1 (fr) * 1991-05-17 1992-11-26 Dana Farber Cancer Institute Techniques de determination de facteurs affectant la circularisation de l'adn, techniques de determination de facteurs affectant l'integration de l'adn, facteurs, et utilisations de ceux-ci
WO1996021156A1 (fr) * 1995-01-04 1996-07-11 Abbott Laboratories Procede de preparation de cibles d'essai de proximite par scintillation

Also Published As

Publication number Publication date
NO20004753D0 (no) 2000-09-22
EP1086242A1 (fr) 2001-03-28
IL138532A0 (en) 2001-10-31
NO20004753L (no) 2000-11-23
AU3269999A (en) 1999-10-18
WO1999049077A1 (fr) 1999-09-30
JP2002507433A (ja) 2002-03-12

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued