CA2319349A1 - Determination de la modification de sequences d'un acide nucleique par analyse du decalage de la terminaison - Google Patents

Determination de la modification de sequences d'un acide nucleique par analyse du decalage de la terminaison Download PDF

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Publication number
CA2319349A1
CA2319349A1 CA002319349A CA2319349A CA2319349A1 CA 2319349 A1 CA2319349 A1 CA 2319349A1 CA 002319349 A CA002319349 A CA 002319349A CA 2319349 A CA2319349 A CA 2319349A CA 2319349 A1 CA2319349 A1 CA 2319349A1
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CA
Canada
Prior art keywords
primer
nucleic acid
nucleotide
terminator
interest
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002319349A
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English (en)
Inventor
Xiao Bing Wang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
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Individual
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Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2319349A1 publication Critical patent/CA2319349A1/fr
Abandoned legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6858Allele-specific amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA002319349A 1999-11-22 2000-09-28 Determination de la modification de sequences d'un acide nucleique par analyse du decalage de la terminaison Abandoned CA2319349A1 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US16689899P 1999-11-22 1999-11-22
US60/166,898 1999-11-22
US61812900A 2000-07-17 2000-07-17
US09/618,129 2000-07-17

Publications (1)

Publication Number Publication Date
CA2319349A1 true CA2319349A1 (fr) 2001-05-22

Family

ID=26862662

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002319349A Abandoned CA2319349A1 (fr) 1999-11-22 2000-09-28 Determination de la modification de sequences d'un acide nucleique par analyse du decalage de la terminaison

Country Status (7)

Country Link
US (1) US20060141503A1 (fr)
EP (1) EP1101824A3 (fr)
JP (1) JP2001245698A (fr)
KR (1) KR20010051353A (fr)
CN (1) CN1297058A (fr)
CA (1) CA2319349A1 (fr)
TW (1) TWI252254B (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6824980B2 (en) * 2000-06-08 2004-11-30 Xiao Bing Wang Isometric primer extension method and kit for detection and quantification of specific nucleic acid
CH699253B1 (de) * 2000-09-18 2010-02-15 Eidgenoessische Forschungsanst Verfahren zur Charakterisierung und/oder Identifikation von Genomen.
JP4214779B2 (ja) * 2001-04-04 2009-01-28 和光純薬工業株式会社 電気泳動法
DE60334132D1 (de) * 2002-05-31 2010-10-21 Nat Inst Of Advanced Ind Scien Neues verfahren zum testen von nukleinsäure unter verwendung von markiertem nukleotid
WO2004003228A1 (fr) * 2002-07-01 2004-01-08 Unisearch Limited Methode de genotypage
FI20075124A0 (fi) * 2007-02-21 2007-02-21 Valtion Teknillinen Menetelmä ja testikitti nukleotidivariaatioiden toteamiseksi
US9181578B2 (en) * 2008-11-18 2015-11-10 Bionano Genomics, Inc. Polynucleotide mapping and sequencing
CN109112193A (zh) * 2013-07-03 2019-01-01 伊鲁米那股份有限公司 正交合成测序
HUE059673T2 (hu) * 2017-01-04 2022-12-28 Mgi Tech Co Ltd Nukleinsav-szekvenálás affinitási reagensek felhasználásával
WO2019070593A1 (fr) * 2017-10-04 2019-04-11 Centrillion Technologies, Inc. Procédé et système de synthèse enzymatique d'oligonucléotides

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4948882A (en) * 1983-02-22 1990-08-14 Syngene, Inc. Single-stranded labelled oligonucleotides, reactive monomers and methods of synthesis
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US5179198A (en) * 1988-07-11 1993-01-12 Hidechika Okada Glycoprotein and gene coding therefor
IL97222A (en) * 1990-02-16 1995-08-31 Orion Yhtymae Oy Method and Responder for Determining Special Changes to Nucleotide
US6013431A (en) * 1990-02-16 2000-01-11 Molecular Tool, Inc. Method for determining specific nucleotide variations by primer extension in the presence of mixture of labeled nucleotides and terminators
US5846710A (en) * 1990-11-02 1998-12-08 St. Louis University Method for the detection of genetic diseases and gene sequence variations by single nucleotide primer extension
US5888819A (en) * 1991-03-05 1999-03-30 Molecular Tool, Inc. Method for determining nucleotide identity through primer extension
WO1993014217A1 (fr) * 1992-01-10 1993-07-22 Life Technologies, Inc. Utilisation de nucleotides predetermines possedant des caracteristiques modifies d'appariement de bases dans l'amplification de molecules d'acide nucleique
US5710028A (en) * 1992-07-02 1998-01-20 Eyal; Nurit Method of quick screening and identification of specific DNA sequences by single nucleotide primer extension and kits therefor
US5650277A (en) * 1992-07-02 1997-07-22 Diagenetics Ltd. Method of determining the presence and quantifying the number of di- and trinucleotide repeats
US6007987A (en) * 1993-08-23 1999-12-28 The Trustees Of Boston University Positional sequencing by hybridization
JPH09505397A (ja) * 1993-11-17 1997-05-27 アマーシャム・インターナショナル・ピーエルシー プライマー伸長質量分光分析による核酸配列決定法
AU5296496A (en) * 1995-03-24 1996-10-16 Mitokor Mutation detection by differential primer extension of mutant and wildtype target sequences
US5830655A (en) * 1995-05-22 1998-11-03 Sri International Oligonucleotide sizing using cleavable primers
US5700642A (en) * 1995-05-22 1997-12-23 Sri International Oligonucleotide sizing using immobilized cleavable primers
US6022688A (en) * 1996-05-13 2000-02-08 Sequenom, Inc. Method for dissociating biotin complexes
US5965363A (en) * 1996-09-19 1999-10-12 Genetrace Systems Inc. Methods of preparing nucleic acids for mass spectrometric analysis
US5885775A (en) * 1996-10-04 1999-03-23 Perseptive Biosystems, Inc. Methods for determining sequences information in polynucleotides using mass spectrometry
US5888778A (en) * 1997-06-16 1999-03-30 Exact Laboratories, Inc. High-throughput screening method for identification of genetic mutations or disease-causing microorganisms using segmented primers
US5994079A (en) * 1998-02-06 1999-11-30 Digene Corporation Direct detection of RNA mediated by reverse transcriptase lacking RNAse H function
US6221592B1 (en) * 1998-10-20 2001-04-24 Wisconsin Alumi Research Foundation Computer-based methods and systems for sequencing of individual nucleic acid molecules
US6156178A (en) * 1999-07-13 2000-12-05 Molecular Dynamics, Inc. Increased throughput analysis of small compounds using multiple temporally spaced injections
US6824980B2 (en) * 2000-06-08 2004-11-30 Xiao Bing Wang Isometric primer extension method and kit for detection and quantification of specific nucleic acid

Also Published As

Publication number Publication date
JP2001245698A (ja) 2001-09-11
EP1101824A3 (fr) 2003-01-08
TWI252254B (en) 2006-04-01
CN1297058A (zh) 2001-05-30
US20060141503A1 (en) 2006-06-29
EP1101824A2 (fr) 2001-05-23
KR20010051353A (ko) 2001-06-25

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EEER Examination request
FZDE Discontinued