CA2319349A1 - Determination de la modification de sequences d'un acide nucleique par analyse du decalage de la terminaison - Google Patents
Determination de la modification de sequences d'un acide nucleique par analyse du decalage de la terminaison Download PDFInfo
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- CA2319349A1 CA2319349A1 CA002319349A CA2319349A CA2319349A1 CA 2319349 A1 CA2319349 A1 CA 2319349A1 CA 002319349 A CA002319349 A CA 002319349A CA 2319349 A CA2319349 A CA 2319349A CA 2319349 A1 CA2319349 A1 CA 2319349A1
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Classifications
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- C—CHEMISTRY; METALLURGY
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
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Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US16689899P | 1999-11-22 | 1999-11-22 | |
US60/166,898 | 1999-11-22 | ||
US61812900A | 2000-07-17 | 2000-07-17 | |
US09/618,129 | 2000-07-17 |
Publications (1)
Publication Number | Publication Date |
---|---|
CA2319349A1 true CA2319349A1 (fr) | 2001-05-22 |
Family
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002319349A Abandoned CA2319349A1 (fr) | 1999-11-22 | 2000-09-28 | Determination de la modification de sequences d'un acide nucleique par analyse du decalage de la terminaison |
Country Status (7)
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US (1) | US20060141503A1 (fr) |
EP (1) | EP1101824A3 (fr) |
JP (1) | JP2001245698A (fr) |
KR (1) | KR20010051353A (fr) |
CN (1) | CN1297058A (fr) |
CA (1) | CA2319349A1 (fr) |
TW (1) | TWI252254B (fr) |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6824980B2 (en) * | 2000-06-08 | 2004-11-30 | Xiao Bing Wang | Isometric primer extension method and kit for detection and quantification of specific nucleic acid |
CH699253B1 (de) * | 2000-09-18 | 2010-02-15 | Eidgenoessische Forschungsanst | Verfahren zur Charakterisierung und/oder Identifikation von Genomen. |
JP4214779B2 (ja) * | 2001-04-04 | 2009-01-28 | 和光純薬工業株式会社 | 電気泳動法 |
DE60334132D1 (de) * | 2002-05-31 | 2010-10-21 | Nat Inst Of Advanced Ind Scien | Neues verfahren zum testen von nukleinsäure unter verwendung von markiertem nukleotid |
WO2004003228A1 (fr) * | 2002-07-01 | 2004-01-08 | Unisearch Limited | Methode de genotypage |
FI20075124A0 (fi) * | 2007-02-21 | 2007-02-21 | Valtion Teknillinen | Menetelmä ja testikitti nukleotidivariaatioiden toteamiseksi |
US9181578B2 (en) * | 2008-11-18 | 2015-11-10 | Bionano Genomics, Inc. | Polynucleotide mapping and sequencing |
CN109112193A (zh) * | 2013-07-03 | 2019-01-01 | 伊鲁米那股份有限公司 | 正交合成测序 |
HUE059673T2 (hu) * | 2017-01-04 | 2022-12-28 | Mgi Tech Co Ltd | Nukleinsav-szekvenálás affinitási reagensek felhasználásával |
WO2019070593A1 (fr) * | 2017-10-04 | 2019-04-11 | Centrillion Technologies, Inc. | Procédé et système de synthèse enzymatique d'oligonucléotides |
Family Cites Families (23)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4948882A (en) * | 1983-02-22 | 1990-08-14 | Syngene, Inc. | Single-stranded labelled oligonucleotides, reactive monomers and methods of synthesis |
US4965188A (en) * | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
US5179198A (en) * | 1988-07-11 | 1993-01-12 | Hidechika Okada | Glycoprotein and gene coding therefor |
IL97222A (en) * | 1990-02-16 | 1995-08-31 | Orion Yhtymae Oy | Method and Responder for Determining Special Changes to Nucleotide |
US6013431A (en) * | 1990-02-16 | 2000-01-11 | Molecular Tool, Inc. | Method for determining specific nucleotide variations by primer extension in the presence of mixture of labeled nucleotides and terminators |
US5846710A (en) * | 1990-11-02 | 1998-12-08 | St. Louis University | Method for the detection of genetic diseases and gene sequence variations by single nucleotide primer extension |
US5888819A (en) * | 1991-03-05 | 1999-03-30 | Molecular Tool, Inc. | Method for determining nucleotide identity through primer extension |
WO1993014217A1 (fr) * | 1992-01-10 | 1993-07-22 | Life Technologies, Inc. | Utilisation de nucleotides predetermines possedant des caracteristiques modifies d'appariement de bases dans l'amplification de molecules d'acide nucleique |
US5710028A (en) * | 1992-07-02 | 1998-01-20 | Eyal; Nurit | Method of quick screening and identification of specific DNA sequences by single nucleotide primer extension and kits therefor |
US5650277A (en) * | 1992-07-02 | 1997-07-22 | Diagenetics Ltd. | Method of determining the presence and quantifying the number of di- and trinucleotide repeats |
US6007987A (en) * | 1993-08-23 | 1999-12-28 | The Trustees Of Boston University | Positional sequencing by hybridization |
JPH09505397A (ja) * | 1993-11-17 | 1997-05-27 | アマーシャム・インターナショナル・ピーエルシー | プライマー伸長質量分光分析による核酸配列決定法 |
AU5296496A (en) * | 1995-03-24 | 1996-10-16 | Mitokor | Mutation detection by differential primer extension of mutant and wildtype target sequences |
US5830655A (en) * | 1995-05-22 | 1998-11-03 | Sri International | Oligonucleotide sizing using cleavable primers |
US5700642A (en) * | 1995-05-22 | 1997-12-23 | Sri International | Oligonucleotide sizing using immobilized cleavable primers |
US6022688A (en) * | 1996-05-13 | 2000-02-08 | Sequenom, Inc. | Method for dissociating biotin complexes |
US5965363A (en) * | 1996-09-19 | 1999-10-12 | Genetrace Systems Inc. | Methods of preparing nucleic acids for mass spectrometric analysis |
US5885775A (en) * | 1996-10-04 | 1999-03-23 | Perseptive Biosystems, Inc. | Methods for determining sequences information in polynucleotides using mass spectrometry |
US5888778A (en) * | 1997-06-16 | 1999-03-30 | Exact Laboratories, Inc. | High-throughput screening method for identification of genetic mutations or disease-causing microorganisms using segmented primers |
US5994079A (en) * | 1998-02-06 | 1999-11-30 | Digene Corporation | Direct detection of RNA mediated by reverse transcriptase lacking RNAse H function |
US6221592B1 (en) * | 1998-10-20 | 2001-04-24 | Wisconsin Alumi Research Foundation | Computer-based methods and systems for sequencing of individual nucleic acid molecules |
US6156178A (en) * | 1999-07-13 | 2000-12-05 | Molecular Dynamics, Inc. | Increased throughput analysis of small compounds using multiple temporally spaced injections |
US6824980B2 (en) * | 2000-06-08 | 2004-11-30 | Xiao Bing Wang | Isometric primer extension method and kit for detection and quantification of specific nucleic acid |
-
2000
- 2000-09-21 JP JP2000286503A patent/JP2001245698A/ja active Pending
- 2000-09-28 CA CA002319349A patent/CA2319349A1/fr not_active Abandoned
- 2000-09-28 EP EP00308509A patent/EP1101824A3/fr not_active Ceased
- 2000-10-30 CN CN00133770A patent/CN1297058A/zh active Pending
- 2000-10-31 KR KR1020000064201A patent/KR20010051353A/ko not_active Application Discontinuation
- 2000-11-08 TW TW089123547A patent/TWI252254B/zh not_active IP Right Cessation
-
2005
- 2005-11-21 US US11/284,453 patent/US20060141503A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
JP2001245698A (ja) | 2001-09-11 |
EP1101824A3 (fr) | 2003-01-08 |
TWI252254B (en) | 2006-04-01 |
CN1297058A (zh) | 2001-05-30 |
US20060141503A1 (en) | 2006-06-29 |
EP1101824A2 (fr) | 2001-05-23 |
KR20010051353A (ko) | 2001-06-25 |
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