CA2271228A1 - Technique de production de genes, de proteines et de transcripts marques - Google Patents

Technique de production de genes, de proteines et de transcripts marques Download PDF

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Publication number
CA2271228A1
CA2271228A1 CA002271228A CA2271228A CA2271228A1 CA 2271228 A1 CA2271228 A1 CA 2271228A1 CA 002271228 A CA002271228 A CA 002271228A CA 2271228 A CA2271228 A CA 2271228A CA 2271228 A1 CA2271228 A1 CA 2271228A1
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CA
Canada
Prior art keywords
gene
sequence
group
cell
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002271228A
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English (en)
Inventor
Jonathan W. Jarvik
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
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Individual
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Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2271228A1 publication Critical patent/CA2271228A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags

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  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Cette invention porte sur une technique au titre de laquelle un marqueur moléculaire est placé sur un gène, un transcript et une protéine, en un seul processus de recombinaison. Le marqueur de protéine prend la forme d'un peptide unique pouvant être reconnu par un anticorps ou un autre réactif spécifique, le marqueur de transcript, celle d'une séquence nucléotidique codant le peptide qu'une sonde polynucléotidique spécifique est en mesure de reconnaître et le marqueur de gène, celle d'une séquence nucléotidique plus longue renfermant la séquence codant le peptide et d'autres séquences nucléotidiques associées. La particularité la plus notable de l'invention réside essentiellement dans le fait que la structure de l'ADN constitutif du marqueur est telle qu'au moment de son insertion dans un intron à l'intérieur d'un gène, il se crée deux introns hybrides séparés par un nouvel exon codant le marqueur de protéine. Le point fort de cette technique est de permettre l'identification de nouvelles protéines ou de structures contenant une protéine et, cela fait, une identification ainsi qu'une analyse aisées du gène codant ces protéines.
CA002271228A 1996-11-08 1997-11-07 Technique de production de genes, de proteines et de transcripts marques Abandoned CA2271228A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US70540496A 1996-11-08 1996-11-08
US08/705,404 1996-11-08
PCT/US1997/020150 WO1998020031A1 (fr) 1996-11-08 1997-11-07 Technique de production de genes, de proteines et de transcripts marques

Publications (1)

Publication Number Publication Date
CA2271228A1 true CA2271228A1 (fr) 1998-05-14

Family

ID=24833313

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002271228A Abandoned CA2271228A1 (fr) 1996-11-08 1997-11-07 Technique de production de genes, de proteines et de transcripts marques

Country Status (3)

Country Link
AU (1) AU5168598A (fr)
CA (1) CA2271228A1 (fr)
WO (1) WO1998020031A1 (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7332338B2 (en) 1996-10-04 2008-02-19 Lexicon Pharmaceuticals, Inc. Vectors for making genomic modifications
US6136566A (en) * 1996-10-04 2000-10-24 Lexicon Graphics Incorporated Indexed library of cells containing genomic modifications and methods of making and utilizing the same
US6207371B1 (en) 1996-10-04 2001-03-27 Lexicon Genetics Incorporated Indexed library of cells containing genomic modifications and methods of making and utilizing the same
US6855545B1 (en) 1996-10-04 2005-02-15 Lexicon Genetics Inc. Indexed library of cells containing genomic modifications and methods of making and utilizing the same
US6080576A (en) * 1998-03-27 2000-06-27 Lexicon Genetics Incorporated Vectors for gene trapping and gene activation
US6808921B1 (en) 1998-03-27 2004-10-26 Lexicon Genetics Incorporated Vectors for gene mutagenesis and gene discovery
US6436707B1 (en) 1998-03-27 2002-08-20 Lexicon Genetics Incorporated Vectors for gene mutagenesis and gene discovery
JP3688118B2 (ja) * 1998-05-22 2005-08-24 独立行政法人科学技術振興機構 遺伝子トラップ用ベクターと、このベクターを用いた遺伝子トラップ方法
WO2002053732A2 (fr) * 2000-12-28 2002-07-11 Pangenex Technique d'elaboration de bibliotheque de polynucleotides, de reseaux de polynucleotides et de bibliotheques de cellules destines a l'analyse des caracteristiques genomiques de haut rendement
EP2245163A2 (fr) * 2008-01-24 2010-11-03 Yeda Research And Development Company Ltd. Populations cellulaires pour analyse de polypeptides et utilisations correspondantes

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5652128A (en) * 1993-01-05 1997-07-29 Jarvik; Jonathan Wallace Method for producing tagged genes, transcripts, and proteins

Also Published As

Publication number Publication date
AU5168598A (en) 1998-05-29
WO1998020031A1 (fr) 1998-05-14

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued