CA2266042A1 - Dna polymerases with enhanced thermostability and enhanced length and efficiency of primer extension - Google Patents
Dna polymerases with enhanced thermostability and enhanced length and efficiency of primer extensionInfo
- Publication number
- CA2266042A1 CA2266042A1 CA002266042A CA2266042A CA2266042A1 CA 2266042 A1 CA2266042 A1 CA 2266042A1 CA 002266042 A CA002266042 A CA 002266042A CA 2266042 A CA2266042 A CA 2266042A CA 2266042 A1 CA2266042 A1 CA 2266042A1
- Authority
- CA
- Canada
- Prior art keywords
- dna
- dna polymerase
- amino acid
- enhanced
- thermostable
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
A DNA polymerase having an amino acid sequence comprising substantially the same amino acid sequence as that of Thermus aquaticus or Thermus flavus DNA polymerase, excluding the N-terminal 280 amino acid residues of Thermus aquaticus DNA polymerase or the N-terminal 279 amino acid residues of Thermus flavus DNA polymerase, recombinant DNA sequences encoding said DNA polymerases, vectors comprising said DNA sequences, and host cells containing such vectors. A formulation of thermostable or other DNA polymerases comprising a majority component comprised of at least one thermostable or other DNA polymerase of the type described above, wherein the DNA polymerase lacks 3'-exonuclease activity, and a minority component comprised of at least one thermostable DNA polymerase exhibiting 3'-exonuclease activity, and an improved method for enzymatic extension of DNA strands, especially while, but not limited to, amplifying nucleic acid sequences by polymerase chain reaction wherein the above formulation is made and used to catalyse primer extension, are also provided.
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/021,623 | 1993-02-19 | ||
| US08/021,623 US5436149A (en) | 1993-02-19 | 1993-02-19 | Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension |
| US20203294A | 1994-02-22 | 1994-02-22 | |
| CA002156176A CA2156176C (en) | 1993-02-19 | 1994-02-22 | Dna polymerases with enhanced thermostability and enhanced length and efficiency of primer extension |
| US08/202,032 | 1994-02-22 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002156176A Division CA2156176C (en) | 1993-02-19 | 1994-02-22 | Dna polymerases with enhanced thermostability and enhanced length and efficiency of primer extension |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2266042A1 true CA2266042A1 (en) | 1994-11-24 |
| CA2266042C CA2266042C (en) | 2001-07-17 |
Family
ID=27170064
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002266042A Expired - Lifetime CA2266042C (en) | 1993-02-19 | 1994-02-22 | Dna polymerases with enhanced thermostability and enhanced length and efficiency of primer extension |
Country Status (1)
| Country | Link |
|---|---|
| CA (1) | CA2266042C (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114015672A (en) * | 2021-12-06 | 2022-02-08 | 江南大学 | Pfu DNA polymerase |
-
1994
- 1994-02-22 CA CA002266042A patent/CA2266042C/en not_active Expired - Lifetime
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114015672A (en) * | 2021-12-06 | 2022-02-08 | 江南大学 | Pfu DNA polymerase |
| CN114015672B (en) * | 2021-12-06 | 2022-05-31 | 江南大学 | Pfu DNA polymerase |
Also Published As
| Publication number | Publication date |
|---|---|
| CA2266042C (en) | 2001-07-17 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKEX | Expiry |
Effective date: 20140224 |