CA2237222A1 - Method and apparatus for identifying fluorophores - Google Patents

Method and apparatus for identifying fluorophores

Info

Publication number
CA2237222A1
CA2237222A1 CA002237222A CA2237222A CA2237222A1 CA 2237222 A1 CA2237222 A1 CA 2237222A1 CA 002237222 A CA002237222 A CA 002237222A CA 2237222 A CA2237222 A CA 2237222A CA 2237222 A1 CA2237222 A1 CA 2237222A1
Authority
CA
Canada
Prior art keywords
fluorophores
fluorophore
fluorescence
present
identifying
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002237222A
Other languages
French (fr)
Other versions
CA2237222C (en
Inventor
Vera B. Gorfinkel
Serge Luryi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Research Foundation of State University of New York
Original Assignee
The Research Foundation Of State University Of New York
Vera B. Gorfinkel
Serge Luryi
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=24241654&utm_source=***_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CA2237222(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by The Research Foundation Of State University Of New York, Vera B. Gorfinkel, Serge Luryi filed Critical The Research Foundation Of State University Of New York
Publication of CA2237222A1 publication Critical patent/CA2237222A1/en
Application granted granted Critical
Publication of CA2237222C publication Critical patent/CA2237222C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6419—Excitation at two or more wavelengths

Abstract

The present invention is a unique method for identifying the presence, and preferably the identity, of a fluorophore by optically stimulating one or more fluorophores with an optical signal which has been modulated in intensity in the time domain. The stimulated fluorophore produces a resulting fluorescence which is demodulated to produce an electrical signal corresponding to the intensity modulation of the fluorescence. Finally, the electrical signal is compared to the modulation of the optical signal to determine whether or not the fluorophore is present. The present method can be used alone or in conjunction with known methods of optically analyzing fluorescence of fluorophores to determine the presence of fluorophores.
CA002237222A 1995-11-21 1996-11-20 Method and apparatus for identifying fluorophores Expired - Fee Related CA2237222C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/561,368 US5784157A (en) 1995-11-21 1995-11-21 Method and apparatus for identifying fluorophores
US08/561,368 1995-11-21
PCT/US1996/018696 WO1997019342A1 (en) 1995-11-21 1996-11-20 Method and apparatus for identifying fluorophores

Publications (2)

Publication Number Publication Date
CA2237222A1 true CA2237222A1 (en) 1997-05-29
CA2237222C CA2237222C (en) 2003-07-01

Family

ID=24241654

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002237222A Expired - Fee Related CA2237222C (en) 1995-11-21 1996-11-20 Method and apparatus for identifying fluorophores

Country Status (7)

Country Link
US (1) US5784157A (en)
EP (1) EP0862735B1 (en)
AT (1) ATE236389T1 (en)
AU (1) AU1080097A (en)
CA (1) CA2237222C (en)
DE (1) DE69627181T2 (en)
WO (1) WO1997019342A1 (en)

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Also Published As

Publication number Publication date
EP0862735B1 (en) 2003-04-02
DE69627181D1 (en) 2003-05-08
US5784157A (en) 1998-07-21
AU1080097A (en) 1997-06-11
ATE236389T1 (en) 2003-04-15
WO1997019342A1 (en) 1997-05-29
EP0862735A1 (en) 1998-09-09
CA2237222C (en) 2003-07-01
DE69627181T2 (en) 2004-01-29

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Effective date: 20161121