CA2236339A1 - Procede de criblage par fluorescence destine a l'identification de ligands - Google Patents
Procede de criblage par fluorescence destine a l'identification de ligands Download PDFInfo
- Publication number
- CA2236339A1 CA2236339A1 CA002236339A CA2236339A CA2236339A1 CA 2236339 A1 CA2236339 A1 CA 2236339A1 CA 002236339 A CA002236339 A CA 002236339A CA 2236339 A CA2236339 A CA 2236339A CA 2236339 A1 CA2236339 A1 CA 2236339A1
- Authority
- CA
- Canada
- Prior art keywords
- target protein
- test
- ligand
- protein
- binding
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Urology & Nephrology (AREA)
- Analytical Chemistry (AREA)
- Wood Science & Technology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Cell Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
L'invention concerne un nouveau procédé d'évaluation de l'efficacité pharmaceutique de composés chimiques. Le procédé décrit permet d'identifier des ligands ayant une valeur thérapeutique en les mettant en présence de protéines cibles et en déterminant leur capacité à empêcher le dépliement partiel de la protéine cible, ceci en mesurant l'émission de la fluorescence d'une sonde sensible à la conformation. Ce procédé diffère sensiblement des procédés connus d'évaluation de nouveaux composés pharmaceutiques en ce que la fonction biochimique de la protéine cible n'a pas besoin d'être connue et en ce que l'existence de ligands connus de la protéine cible n'est pas une nécessité.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US834695P | 1995-12-07 | 1995-12-07 | |
US60/008,346 | 1995-12-07 |
Publications (1)
Publication Number | Publication Date |
---|---|
CA2236339A1 true CA2236339A1 (fr) | 1997-06-12 |
Family
ID=21731102
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002236339A Abandoned CA2236339A1 (fr) | 1995-12-07 | 1996-12-06 | Procede de criblage par fluorescence destine a l'identification de ligands |
Country Status (5)
Country | Link |
---|---|
EP (1) | EP0865502A4 (fr) |
JP (1) | JP2000502440A (fr) |
CA (1) | CA2236339A1 (fr) |
DE (1) | DE865502T1 (fr) |
WO (1) | WO1997020952A1 (fr) |
Families Citing this family (24)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2184195C (fr) | 1995-10-25 | 2002-04-16 | Andrew Pakula | Methode d'analyse pour la caracterisation de ligands de proteines cibles |
KR20000011069A (ko) | 1996-05-09 | 2000-02-25 | 스리-디멘셔널 파마슈티컬스 인코포레이티드 | 리간드 개발 및 다변수 단백질 화학의 최적화를 위한미량이식판 열 이동 분석 및 그 장치 |
CA2270527A1 (fr) | 1996-11-04 | 1998-05-14 | 3-Dimensional Pharmaceuticals, Inc. | Systeme, procede et produit programme informatique pour visualiser, traiter et analyser de facon interactive des donnees chimiques |
US6453246B1 (en) | 1996-11-04 | 2002-09-17 | 3-Dimensional Pharmaceuticals, Inc. | System, method, and computer program product for representing proximity data in a multi-dimensional space |
US6571227B1 (en) | 1996-11-04 | 2003-05-27 | 3-Dimensional Pharmaceuticals, Inc. | Method, system and computer program product for non-linear mapping of multi-dimensional data |
US6261842B1 (en) | 1997-10-23 | 2001-07-17 | Wisconsin Alumni Research Foundation | Microorganism genomics, compositions and methods related thereto |
HU225767B1 (en) * | 1997-11-12 | 2007-08-28 | Dimensional Pharmaceuticals 3 | High throughput method for functionally classifying proteins identified using a genomics approach |
US6647358B2 (en) | 1998-09-14 | 2003-11-11 | Lion Bioscience Ag | Pharmacokinetic-based drug design tool and method |
US6569631B1 (en) | 1998-11-12 | 2003-05-27 | 3-Dimensional Pharmaceuticals, Inc. | Microplate thermal shift assay for ligand development using 5-(4″dimethylaminophenyl)-2-(4′-phenyl)oxazole derivative fluorescent dyes |
JP2002535989A (ja) | 1999-02-04 | 2002-10-29 | アナディーズ・ファーマスーティカルズ,インコーポレイテッド | カンジダ・アルビカンスsrb‐7 |
US6972198B2 (en) | 1999-02-26 | 2005-12-06 | Cyclacel, Ltd. | Methods and compositions using protein binding partners |
US6582907B1 (en) * | 1999-12-09 | 2003-06-24 | Pharmacia & Upjohn Company | Use of fluorescence correlation spectroscopy to identify compounds that bind to target species under isothermal denaturing conditions |
US6376180B1 (en) * | 1999-12-09 | 2002-04-23 | Pharmacia & Upjohn Company | Methods of identifying compounds that bind to target species under isothermal denaturing conditions |
US7416524B1 (en) | 2000-02-18 | 2008-08-26 | Johnson & Johnson Pharmaceutical Research & Development, L.L.C. | System, method and computer program product for fast and efficient searching of large chemical libraries |
WO2001065462A2 (fr) | 2000-02-29 | 2001-09-07 | 3-Dimensional Pharmaceuticals, Inc. | Procede et produit de programme informatique de conception de tableaux combinatoires |
WO2001071624A1 (fr) | 2000-03-22 | 2001-09-27 | 3-Dimensional Pharmaceuticals, Inc. | Systeme, procede et progiciel pour la representation de relations entre objets dans un espace multidimensionnel |
US7139739B2 (en) | 2000-04-03 | 2006-11-21 | Johnson & Johnson Pharmaceutical Research & Development, L.L.C. | Method, system, and computer program product for representing object relationships in a multidimensional space |
US7054757B2 (en) | 2001-01-29 | 2006-05-30 | Johnson & Johnson Pharmaceutical Research & Development, L.L.C. | Method, system, and computer program product for analyzing combinatorial libraries |
WO2002103321A2 (fr) * | 2001-06-14 | 2002-12-27 | Anadys Pharmaceuticals, Inc. | Procedes de criblage de ligands de molecules cibles |
EP1637885A1 (fr) * | 2004-09-16 | 2006-03-22 | Vivalis | Méthode de criblage en employant des peptides sensibles à la conformation de protéines |
GB0421285D0 (en) | 2004-09-24 | 2004-10-27 | Univ Nottingham | Improvements in high content screening |
US8158340B2 (en) | 2005-12-28 | 2012-04-17 | Corning Incorporated | Methods for detecting conformational changes in bioentities |
WO2016127100A1 (fr) | 2015-02-05 | 2016-08-11 | Promega Corporation | Analyses à variation thermique fondées sur la luciférase |
GB202118173D0 (en) * | 2021-12-15 | 2022-01-26 | Medicines Discovery Catpult Ltd | Protein stability assay |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5231755B2 (fr) * | 1973-01-08 | 1977-08-17 | ||
US5200504A (en) * | 1990-10-02 | 1993-04-06 | The Scripps Research Institute | Metallopeptides having stabilized secondary structures |
CA2115900A1 (fr) * | 1993-02-22 | 1994-08-23 | Gerald W. Becker | Cribles pharmaceutiques et anticorps |
-
1996
- 1996-12-06 JP JP9521492A patent/JP2000502440A/ja active Pending
- 1996-12-06 DE DE0865502T patent/DE865502T1/de active Pending
- 1996-12-06 CA CA002236339A patent/CA2236339A1/fr not_active Abandoned
- 1996-12-06 EP EP96944304A patent/EP0865502A4/fr not_active Withdrawn
- 1996-12-06 WO PCT/US1996/019698 patent/WO1997020952A1/fr not_active Application Discontinuation
Also Published As
Publication number | Publication date |
---|---|
EP0865502A1 (fr) | 1998-09-23 |
JP2000502440A (ja) | 2000-02-29 |
DE865502T1 (de) | 1999-08-19 |
WO1997020952A1 (fr) | 1997-06-12 |
EP0865502A4 (fr) | 2000-08-16 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FZDE | Dead |