CA2216055A1 - Milieu favorisant la survie des cellules secretrices d'insuline - Google Patents
Milieu favorisant la survie des cellules secretrices d'insuline Download PDFInfo
- Publication number
- CA2216055A1 CA2216055A1 CA 2216055 CA2216055A CA2216055A1 CA 2216055 A1 CA2216055 A1 CA 2216055A1 CA 2216055 CA2216055 CA 2216055 CA 2216055 A CA2216055 A CA 2216055A CA 2216055 A1 CA2216055 A1 CA 2216055A1
- Authority
- CA
- Canada
- Prior art keywords
- culture medium
- medium
- islet
- islets
- ngf
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 210000004153 islets of langerhan Anatomy 0.000 title claims abstract description 24
- 230000004083 survival effect Effects 0.000 title claims abstract description 18
- 239000001963 growth medium Substances 0.000 claims abstract description 23
- 230000000694 effects Effects 0.000 claims abstract description 12
- 239000003102 growth factor Substances 0.000 claims abstract description 12
- 230000006909 anti-apoptosis Effects 0.000 claims abstract description 3
- 230000001737 promoting effect Effects 0.000 claims abstract description 3
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 claims description 24
- 239000002609 medium Substances 0.000 claims description 13
- 102000004877 Insulin Human genes 0.000 claims description 12
- 108090001061 Insulin Proteins 0.000 claims description 12
- 229940125396 insulin Drugs 0.000 claims description 12
- 108090000723 Insulin-Like Growth Factor I Proteins 0.000 claims description 10
- QJJXYPPXXYFBGM-LFZNUXCKSA-N Tacrolimus Chemical compound C1C[C@@H](O)[C@H](OC)C[C@@H]1\C=C(/C)[C@@H]1[C@H](C)[C@@H](O)CC(=O)[C@H](CC=C)/C=C(C)/C[C@H](C)C[C@H](OC)[C@H]([C@H](C[C@H]2C)OC)O[C@@]2(O)C(=O)C(=O)N2CCCC[C@H]2C(=O)O1 QJJXYPPXXYFBGM-LFZNUXCKSA-N 0.000 claims description 8
- 239000003018 immunosuppressive agent Substances 0.000 claims description 7
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- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
- 241000282472 Canis lupus familiaris Species 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
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- 206010033645 Pancreatitis Diseases 0.000 description 1
- 101710111747 Peptidyl-prolyl cis-trans isomerase FKBP12 Proteins 0.000 description 1
- 102100027913 Peptidyl-prolyl cis-trans isomerase FKBP1A Human genes 0.000 description 1
- 101710111682 Peptidyl-prolyl cis-trans isomerase FKBP1A Proteins 0.000 description 1
- 241000288906 Primates Species 0.000 description 1
- 108700039882 Protein Glutamine gamma Glutamyltransferase 2 Proteins 0.000 description 1
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- 229940125721 immunosuppressive agent Drugs 0.000 description 1
- 238000002513 implantation Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
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- 102000006495 integrins Human genes 0.000 description 1
- 108010044426 integrins Proteins 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
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- 238000000034 method Methods 0.000 description 1
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- 238000012986 modification Methods 0.000 description 1
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- 230000000877 morphologic effect Effects 0.000 description 1
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- 230000002276 neurotropic effect Effects 0.000 description 1
- 230000001019 normoglycemic effect Effects 0.000 description 1
- 238000011580 nude mouse model Methods 0.000 description 1
- 210000000277 pancreatic duct Anatomy 0.000 description 1
- 210000004923 pancreatic tissue Anatomy 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 210000003240 portal vein Anatomy 0.000 description 1
- 230000029537 positive regulation of insulin secretion Effects 0.000 description 1
- 230000035935 pregnancy Effects 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0676—Pancreatic cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/105—Insulin-like growth factors [IGF]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/13—Nerve growth factor [NGF]; Brain-derived neurotrophic factor [BDNF]; Cilliary neurotrophic factor [CNTF]; Glial-derived neurotrophic factor [GDNF]; Neurotrophins [NT]; Neuregulins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/22—Coculture with; Conditioned medium produced by pancreatic cells
Landscapes
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Priority Applications (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CA 2216055 CA2216055A1 (fr) | 1997-09-19 | 1997-09-19 | Milieu favorisant la survie des cellules secretrices d'insuline |
AU91498/98A AU9149898A (en) | 1997-09-19 | 1998-09-17 | A medium to promote islet cell survival |
PCT/CA1998/000887 WO1999015625A1 (fr) | 1997-09-19 | 1998-09-17 | Milieu favorisant la survie des cellules insulaires |
CA002304381A CA2304381A1 (fr) | 1997-09-19 | 1998-09-17 | Milieu favorisant la survie des cellules insulaires |
US10/138,517 US6562620B2 (en) | 1997-09-19 | 2002-05-06 | Medium to promote islet cell survival |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CA 2216055 CA2216055A1 (fr) | 1997-09-19 | 1997-09-19 | Milieu favorisant la survie des cellules secretrices d'insuline |
Publications (1)
Publication Number | Publication Date |
---|---|
CA2216055A1 true CA2216055A1 (fr) | 1999-03-19 |
Family
ID=4161504
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA 2216055 Abandoned CA2216055A1 (fr) | 1997-09-19 | 1997-09-19 | Milieu favorisant la survie des cellules secretrices d'insuline |
Country Status (3)
Country | Link |
---|---|
AU (1) | AU9149898A (fr) |
CA (1) | CA2216055A1 (fr) |
WO (1) | WO1999015625A1 (fr) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1248640A1 (fr) * | 2000-01-20 | 2002-10-16 | Diatranz Limited | Preparation et xenotransplantation d'ilots de porc |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5587309A (en) * | 1994-04-29 | 1996-12-24 | The United States Of America As Represented By The Department Of Health And Human Services | Method of stimulating proliferation and differentiation of human fetal pancreatic cells ex vivo |
DE19540080A1 (de) * | 1995-10-27 | 1997-04-30 | Bayer Ag | 3-Aryl-5-halogen-pyron-Derivate |
-
1997
- 1997-09-19 CA CA 2216055 patent/CA2216055A1/fr not_active Abandoned
-
1998
- 1998-09-17 AU AU91498/98A patent/AU9149898A/en not_active Abandoned
- 1998-09-17 WO PCT/CA1998/000887 patent/WO1999015625A1/fr active Application Filing
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1248640A1 (fr) * | 2000-01-20 | 2002-10-16 | Diatranz Limited | Preparation et xenotransplantation d'ilots de porc |
EP1248640A4 (fr) * | 2000-01-20 | 2003-05-21 | Diatranz Ltd | Preparation et xenotransplantation d'ilots de porc |
US7122177B2 (en) | 2000-01-20 | 2006-10-17 | Diabcell Pty Ltd. | Preparation and xenotransplantation of porcine islets |
US7323323B2 (en) | 2000-01-20 | 2008-01-29 | Diabell Pty Ltd. | Preparation and xenotransplantation of porcine islets |
Also Published As
Publication number | Publication date |
---|---|
WO1999015625A1 (fr) | 1999-04-01 |
AU9149898A (en) | 1999-04-12 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FZDE | Dead |