CA2181569C - Ep2-receptor agonists as agents for lowering intraocular pressure - Google Patents

Abstract

The invention relates to (~) trans- 2-[-4(1-hydroxyhexyl) phenyl]-5-oxocyclopentaneheptanoicacid, and ester and unsaturated derivatives thereof, prostaglandin derivatives. More particularly, the present invention concerns compounds of formula (I), wherein the wavy bonds indicate the .alpha. or .beta. configuration, the dashed bond represents either a single or double bond, which double bond may be a cis or trans double bond and R is a saturated or unsaturated acyclic hydrocarbon group having from 1 to about 20 carbon atoms, or -(CH2)mR1- wherein m is 0-10, and R1 is an aliphatic ring having from about 3 to about 7 carbon atoms, or an aryl or heteroaryl ring having from about 4 to about 10 carbon atoms and wherein the heteroatom is selected from the group consisting of N, O and S. The compounds of the present invention are potent ocular hypotensives, and are particularly suitable for the management of glaucoma.

Description

WO 95/1996.1 PCT/US95/00288 INTRAOCULAR PRESSURE
Field of the Invention The present invention relates to the use of EP2 receptor agonists to lower the intraocular pressure of mammals and thus are useful in treating glaucoma. In particular, (~) trans-2-[-4(1-hydroxyhexyl) phenyl]-5-oxocyclopentaneheptanoic-acid, and ester and unsaturated derivatives thereof, are effective for the management of glaucoma.
Background of the Invention Ocular hypotensive. agents are useful in the treatment of a number of various e~cular hypertensive conditions, such as post-surgical and post-laser trabeculectomy ocular hypertensive episodes, glaucoma, and as presurgical adjuncts.
Glaucoma is a disease of the eye characterized by increased intraocular pressure. On the basis of its etiology, glaucoma has been classified as primary or secondary. For example, primary glaucoma in adults (congenital glaucoma) 2 5 may be either open-angle or acute or chronic angle-closure.
Secondary glaucoma results from pre-existing ocular diseases -such as uveitis, intraocular tumor or an enlarged cataract.
The underlying causes of primary glaucoma are not yet 3 0 known. The increased intraocular tension is due to the obstruction of aqueous humor outflow. In chronic open-angle glaucoma, the anterior chamber and its anatomic structures . appear normal, but drainage of the aqueous humor is impeded.
In acute or chronic angle-closure glaucoma, the anterior . 3 5 chamber is shallow, the filtration angle is narrowed, and the iris may obstruct the trabecular meshwork at the entrance of the canal of Schlemm. Dilation of the pupil may push the root W0951199G4 ~ , PCTlUS95100288 of the iris forward against the angle, and may produce pupillary block and thus precipitate an acute attack. Eyes with narrow anterior chamber angles are predisposed to acute angle-closure glaucoma attacks of various degrees of severity.
' Secondary glaucoma is caused by any interference with the flow of aqueous humor from the posterior chamber into ' the anterior chamber and subsequently, into the canal of Schlemm. Inflammatory disease of the anterior segment may 1 0 prevent aqueous escape by causing complete posterior synechia in iris bombe and may plug the drainage channel with exudates. Other common causes are intraocular tumors, enlarged cataracts, central retinal vein occlusion, trauma to the eye, operative procedures and intraocular hemorrhage.
Considering all types together, glaucoma occurs in about 2°l0 of all persons over the age of 40 and may be asymptotic for years before progressing to rapid loss of vision. In cases where surgery is not indicated, topical ~i-adrenoreceptor 2 0 antagonists have traditionally been the drugs of choice for treating glaucoma.
Prostaglandins were earlier regarded as potent ocular hypertensives; however, ev idence accumulated in the last two decades shows that some prostaglandins are highly effective ocular hypotensive agents and are ideally suited for the long-term medical management of glaucoma. (See, for example, Starr, M.S. Ex~E_ye Res. 1971, 11, pp. 170-177; Bito, L. Z.

Biological Protection with Prosta~landins Cohen, M. M., ed., 3 0 Boca Raton, Fla, CRC PressInc., 1985, pp. 231-252; and Bito, L.

Z., Applied Pharmacolog y in the Medical Treatment of Glaucomas Drance, S. M. and Neufeld, A. H. eds., New York, Grune & Stratton, 1984, pp. 477-505). Such prostaglandins _ include PGF2a, PGFla, P GE2, and certain lipid-soluble esters, such as C1 to Cg alkyl esters, e.g. 1-isopropyl ester, of such ' compounds.

W095/1996.t ~ ~ PCTlUS95100288 In the United States Patent No. 4,599,353 certain prostaglandins, in particular PGE2 and-PGF2a and the C1 to CS
alkyl esters of the latter compound, were reported to possess ocular hypotensive activity and were recommended for use in glaucoma management.
Although the precise mechanism is not yet known, recent experimental resvults indicate that the prostaglandin-induced reduction in intraocular pressure results from increased uveoscleral outflow [Nilsson et al., I n v a s t .
Ophthalmol. Vis Scs ~$(suppI), 284 (1987)].
The isopropyl ester of PGF2a has been shown to have significantly greater hypotensive potency than the parent compound, which was attributed to its more effective penetration through the cornea. In 1987 this compound was described as "the most potent ocular hypotensive agent ever reported." [See, for example, Bito, L. Z., Arch. Onhthalmol ~, 1036 (1987), and Siebold et al., Prodrue ~, 3 (1989)].
Whereas prostaglandins appear to be devoid of significant intraocular side effects, ocular surface (conjunctival) hyperemia and foreign-body sensation have 2 5 been consistently associated with the topical ocular use of such compounds, in particular PGF2a and its prodrugs, e.g. its (-isopropyl ester, in humans. The clinical potential of prostaglandins in the management of conditions associated with increased ocular pressure, e.g. glaucoma, is greatly 3 0 limited by these side effects.
Certain phenyl and phenoxy mono, tri and tetra nor prostaglandins and their 1-esters are disclosed in European . Patent Application 0,364,417 as useful in the treatment of 3 5 glaucoma or ocular hypertension.

WO 9$!19964 PCT/US95l00288 Finally, certain EP2-receptor agonists are disclosed in Nials et al, Cardiovascular Drug Reviews, Vol. 11, No. 2, pp.
165-179, Coleman et al, Comprehensive Medicinal Chemistry, Vol. 3, pp. 643-714, 1990 and Woodward et al, Prostaglandins., pp. 371-383, 1993.
Summary of the Tnvention We have found that certain EP2-receptor agonists are potent ocular hypotens~ve agents. We have further found that traps-2-[-4(1-hydroxyhexyl) phenyl]-5-oxocyclopentaneheptanoicacid, and ester and unsaturated derivatives thereof, are especially useful in the treatment of glaucoma and surprisingly, cause no or significantly lower ocular surface hyperemia than the other compounds that are useful in lowering intraocular pressure, e.g. PGF2a and lower alkyl esters thereof.

S
The present invention relates to methods of treating ocular hypertension which comprises administering an effective amount of a compound represented by the formula I
,O
OR
OH
wherein, the wavy bands indicate the a or (3 configuration, R is a saturated or unsaturated acyclic hydrocarbon group having 1 0 from I to about 20 carbon atoms, or -(CH2)mR 1 wherein m is 0-10, and RI is an aliphatic ring having from about 3 to about 7 carbon atoms, or an aryl or heteroaryl ring having from about 4 to about 10 carbon atoms, e.g. R1 may be cyclohexyl, phenyl, thienyl, pyridyl or furanyl, or a pharmaceutically 1 5 acceptable salt thereof and the dashed bond represents either a single or double bond which may be in the cis or trans position. Preferably R1 is lower alkyl.
More preferably the method of the present invention 20 comprises administering a compound represented by the formula II

WO 95/1996.1 ~ ~ ~ ~ ~ ~ ~ ~ PCTlUS95100288 ., ° 6 OH
wherein R2 is a lower alkyl radical and the other symbols are as defined above.
In a further aspect, the present invention relates to pharmaceutical compositions comprising a therapeutically effective amount of a compound of formulae (I) or (II) wherein the symbols have the above meanings, or a pharmaceutically acceptable salt thereof in admixture with a non-toxic, pharmaceutically acceptable liquid vehicle.
In a still further aspect, the present invention relates to certain (t) trans-2-[-4(1-hydroxyhexyl) phenyl]-5-oxocyclo-1 S pentaneheptanoicacid, and ester and unsaturated derivatives thereof, of the above formulae, wherein the substituents and symbols are as defined hereinabove, or a pharmaceutically acceptable salt of such compounds.

W0 95It9964 PCT/US95100288 Detailed Description of the Invention The present invention relates to the use of (t) traps-2-[-4(1-hydroxyhexyl) phenyl]-5-oxocyclopentaneheptanoic- acid, ' S and ester and unsaturated derivatives thereof, as ocular hypotensives. These therapeutic agents are represented by compounds having the formula I
n OR
OH
as defined above. The preferred compounds used in accordance with the present invention are encompassed by the following structural formula II

WO 95/19964 ~ ~ PCT/US95I00288 .O . ~, OH
wherein R2 is a lower alkyl radical.
In all of the above formulae,as well as in those provided hereinafter, the straight lines Where there represent bonds. is no symbol for the atoms between the bonds,the appropriate carbon-containing inferred. For example radical is to be in formula I, the radical between cyclopentylring and the the OR
O
radical is a polymethylene (CH2) radical, i.e. a hexylenyl radical. The dotted lines on the bond between carbons 5 and 6 (C-5), indicate a single or a double bond which can be in the cis or trans configuration. The radical adjacent the double bond is a CH radical. If two solid lines are used that indicates a specific configuration for that double bond. Hatched lines at positions C-9 and C-I1 indicate the a configuration. If one 2 0 were to draw the (3 configuration, a solid triangular line would be used.

In the compounds used in accordance with the present invention, compounds having the C-9 or C-11 substituents in the a or (i configuration are contemplated. As hereinabove mentioned, in all formulas provided herein broken line attachments to the cyclopentane ring indicate substituents in the a configuration. Thickened solid line attachments to the cyclopentane ring indicate substituents in the (i configuration.
For the purpose of this invention, unless further limited, the term "alkyl" refers to alkyl groups having from one to ten carbon atoms and includes "lower alkyl" radicals having from one to five carbon atoms, the term "cycloalkyl" refers to cycloalkyl groups having from three to seven carbon atoms, the term "aryl" refers to aryl groups having from four to ten carbon atoms. The term "saturated or unsaturated acyclic hydrocarbon group" i s used to refer to straight or branched chain, saturated or unsaturated hydrocarbon groups having from one to about 6, preferably one to about 4 carbon atoms.

Such groups include alkyl, alkenyl and alkynyl groups of 2 0 appropriate lengths, and preferably are alkyl, e.g. methyl, ethyl, propyl, butyl, pentyl, or hexyl, or an isomeric form thereof.

'The definition of R may include a cyclic component, -(CH~)I"R1, wherein m is 0-10, RZ is an aliphatic ring from about 3 to about 7 carbon atoms, or an aromatic or heteroaromatic ring. The "aliphatic ring" may be saturated or unsaturated, and preferably is a saturated ring having 3-7 carbon atoms, inclusive. As an aromatic ring, Rl preferably is 3 0 phenyl, and the heteroaromatic rings have oxygen, nitrogen or sulfur as a heteroatom, i.e., Rl may be thienyl, furanyl, pyridyl, etc. Preferably m is 0-4.
Preferred representatives of the compounds within the 3 5 scope of the present invention are (~) trans-2-[-4(1-hydroxyhexyl)phenyl]-5-oxocyclopentaneheptanoicacid, WO 951199G.i ~ ~ ~ ~ ~ ~ ~ - ' - PCTlUS95100288 unsaturated derivatives thereof, and lower alkyl esters of Lhese compounds.
The following compound m~~y be used in the 5 pharmaceutical compositions , and the methods of treatment of ., .
the present invention are''(+_) trans-2-[-4(1-hydroxyhexyl) phenyl]-5-oxocyclopentaneheptanoicacid.
A pharmaceutically acceptable salt is any salt which 10 retains the activity of the parent compound and does not impart any deleterious or undesirable effect on the subject to whom it is administered and in the context in which it is administered. Such salts are those formed with pharmaceutically acceptable canons, e.g., alkali metals, alkali earth metals, etc.
Pharmaceutical compositions may be prepared by combining a therapeutically effective amount of at least one compound according to the present invention, or a 2 0 pharmaceutically acceptable salt thereof, as an active ingredient, with conventional ophthalmically acceptable pharmaceutical excipients, and by preparation of unit dosage forms suitable for topical ocular use. The therapeutically efficient amount typically is between about 0.0001 and about 2 5 5% (w/v), preferably about 0.001 to about 1.0% (w/v) in liquid formulations.
For ophthalmic application, preferably solutions are prepared using a physiological saline solution as a major 3 0 vehicle. The pH of such ophthalmic solutions should preferably be maintained between 4.5 and 8.0 with an appropriate buffer system, a neutral pH being preferred but not essential. The formulations may also contain conventional, pharmaceutically acceptable preservatives, stabilizers and 3 5 surfactants.

Preferred preservatives that may be used in the pharmaceutical compositions of the present invention include, but are not limited to, benzalkonium chloride, chlorobutanol, thimerosal, phenylmercuric acetate and phenylmercuric nitrate. A preferred surfactant is, for example, Tween 80.
Likewise, various preferred vehicles may be used in the ophthalmic preparations of the present invention. These vehicles include, but are not limited to, polyvinyl alcohol, povidone, hydroxypropyl methyl cellulose, poloxamers, carboxymethyl cellulose, hydroxyethyl cellulose cyclodextrin and purified water.
Tonicity adjustors may be added as needed or convenient. They include, but are not limited to, salts, particularly sodium chloride, potassium chloride, mannitol and glycerin, or any other suitable ophthalmically acceptable tonicity adjustor.
Various buffers and means for adjusting pH may be used 2 0 so long as the resulting preparation is ophthalmically acceptable. Accordingly, buffers include acetate buffers, citrate buffers, phosphate buffers and borate buffers. Acids or bases may be used to adjust the pH of these formulations as needed.
In a similar vein, an ophthalmically acceptable antioxidant for use in the present invention includes, but is not limited to, sodium metabisulfite, sodium thiosulfate, acetylcysteine, butylated hydroxyanisole and butylated 3 0 hydroxytoluene. ' Other excipient components which may be included in the ophthalmic preparations are chelating agents. The preferred chelatiz~g agent is edentate disodium, although other 3 5 chelating agents may also be used in place of or in conjunction with it.
* Trade-mark WO 951199G.~ ~" ~, PCTIUS95100288 The ingredients are usually used in the following amounts:
>~gredient Amount f% w/vl active ingredientabout 0.001-5 preservative 0-0.10 vehicle 0-40 --tonicity adjustor0-1,0:;

buffer 0.01-10 pH adjustor q.s. pH 4.5-8.0 antioxidant as needed surfactant as needed purified water as needed to make 100%

The actual dose of the active compounds of the present invention depends on the specific compound, and on the condition to be treated; the selection of the appropriate dose is well within the knowledge of the skilled artisan.
The ophthalmic formulations of the present invention are conveniently packaged in forms suitable for metered application, such as in containers equipped with a dropper, to facilitate application to the eye. Containers suitable for dropwise application are usually made of suitable inert, non-toxic plastic material, and generally contain between about 0.5 and about 15 ml solution. One package may contain one or more unit doses.
Especially preservative-free solutions are often 2 0 formulated in non-resealable containers containing up to about ten, preferably up to about five units doses, where a typical unit dose is from one to about 8 drops, preferably one to about 3 drops. The volume of one drop usually is about 20-35 p 1.
2 5 The invention is further illustrated by the following non-Iimiting Examples.

W0 95l199G4 PCT1U595/00288 Exam In a 1 (~) TRANS-2-[-4(1-HYDROXYHEXYL) PHENYL]-5 OXOCYCLO- PENTANEHEPTANOICACID AND LOWER
S ALKS'L ESTERS THEREOF
The above acid compound is well known and may be purchased or synthesized by methods known in the art. The lower alkyl esters of this compound may be made by the esterification procedures described in the various patent applications described in the Background of the Invention.
Intraocular pressure was measured by pneumatonometry in normal monkeys. Studies were performed in conscious animals trained to accept pneumatonometry. The compound of Example 1 was 2 0 administered topically to one eye in a 25 p l volume drop, the contralateral eye received vehicle as a control. Statistical analysis was by Student's paired t test.
The intraocular pressure results are summarized in 2 5 Table 1.

W095/199G-t ~ ~ PCT/US95100288 TABLE 1. EFFECT OF (~) TRANS-2-[-4(1-HYDROXYHEXYL) PHENYL]-S-OXOCYCLO PENTANE
HEPTANOIC ACID, 0.1%, B.LD. ON THE INTRAOCULAR ' PRESSURE OF NORMAL MONKEYS
TIME(HR) RELATIVE TO NET CHANGE IN

FIRST DOSE PRESSURE(MMHg~

2 -1.17 4 -2.0 6 -1.50 24 -0.67 1 26 -1.0 28 -1.83 30 -1.50 48 -1.50 50 -2.33 2 52 -1.50 54 -1.50 72 -2.33 74 -2.17 76 -2.33 2 78 -2.0 S

96 -1.83 98 -2.0 100 -2.0 102 -2.0 (all changes in intraocular pressure are significant p < 0.01 according to Students t test) Example 3 3 5 Intraocular Pressure Reduction After Laser Treatment Intraocular pressure reduction was also achieved in laser-induced ocular hypertensive monkeys. Ocular hypertension was induced by photocoagulating the trabecular meshwork by circumferential argon laser treatment.

TABLE 2. EFFECT OF A SINGLE DOSE (0.1%) OF TRANS-2-[-4(1-HYDROXYHEXYL)PHENYL]-5-OXO-ON LASER-INDUCED OCULAR HYPERTENSIVE MONKEYS
TIME (HR) RELATIVE CHANGE IN INTRA-TO FIRST DOSE OCULAR PRESSURE (MMHg) 1 -4.5 2 -6.0**
4 -4.7**
6 -4.5 * p < 0.05 ** p < 0.01 (Students t test) xa ple 4 2 0 Determination of EPZ Receptor Activity E P 2 receptor activity may be measured in accordance with the procedure disclosed in Woodward et al, Prostaglandins, pp. 371-383, 1993.
The foregoing description details specific methods and compositions that can be employed to practice the present invention, and represents the best mode contemplated.
3 0 However, it is apparent from one of ordinary skill in the art that different pharmaceutical compositions may be prepared and used with substantially the same results. That is other EP2-receptor agonists, such as 19R(OH)PGE2, butaprost, etc.
will effectively lower intraocular pressure in animals and are 3 5 within the broad scope of the present invention.

Claims (11)

Claims:

1, Use for treating ocular hypertension of a compound of formula I
wherein the wavy bonds indicate that .alpha. or .beta. configuration, the dashed bond represents either a single or double bond, which double bond may be a cis or trans double bond and R is a saturated or unsaturated acyclic hydrocarbon group having from 1 to 20 carbon atoms, or -(CH2)m R1 wherein m is 0-10, and R1 is an aliphatic ring having from 3 to 7 carbon atoms, or an aryl or heteroaryl ring having from 4 to 10 carbon atoms and wherein the heteroatom is selected from the group consisting of N, O and S.

2. The use of Claim 1 wherein said compound is a prostaglandin derivative of the formula II

wherein R2 is a lower alkyl radical, the hatched line indicates the a configuration and the solid triangular line indicates the .beta.
configuration.

3. The use of (~) trans-2-(-4(1-hydroxyhexyl) phenyl]-5-oxocyclopentane-heptanoicacid for treating glaucoma.

4. A pharmaceutical composition comprising a compound of formula I

wherein the wavy bonds indicate that .alpha. or .beta. configuration, the dashed bond represents either a single or double bond, which double bond may be a cis or trans double bond and R is a saturated or unsaturated acyclic hydrocarbon group having from 1 to 20 carbon atoms, or -(CH2)m R1 wherein m is 0-10, and R1 is an aliphatic ring having from 3 to 7 carbon atoms, or an aryl or heteroaryl ring having from 4 to 10 carbon atoms and wherein the heteroatom is selected from the group consisting of N, O and S, and an acceptable carrier therefor.

5. An ophthalmic solution comprising a compound of formula I

wherein the wavy bonds indicate that .alpha. or .beta. configuration, the dashed bond represents either a single or double bond, which double bond may be a cis or trans double bond and R is a saturated or unsaturated acyclic hydrocarbon group having from 1 to about 20 carbon atoms, or -(CH2)m R1 wherein m is 0-10, and R1 is an aliphatic ring having from about 3 to about 7 carbon atoms, or an aryl or heteroaryl ring having from about 4 to about 10 carbon atoms and wherein the heteroatom is selected from the group consisting of N, O and S, and an acceptable carrier therefor.

6 The ophthalmic solution of Claim 5 comprising at least one ingredient selected from the group of an ophthalmically acceptable preservative, buffer system, antioxidant and chelating agent.

7. The ophthalmic solution of Claim 6 wherein said compound is a prostaglandin derivative of the formula II

wherein R2 is a lower alkyl radical, the hatched line indicates the a configuration and the solid triangular line indicates the .beta.
configuration.

8. An ophthalmic solution comprising (~) trans-2-[-4(1-hydroxyhexyl) phenyl]-5-oxocyclopentaneheptanoicacid and an acceptable carrier therefor.

9. A pharmaceutical product, comprising a container adapted to dispense its contents in metered form; and an ophthalmic solution therein, as defined in Claim 5.

10. A compound of the formula I

wherein the wavy bonds indicate that .alpha. or .beta. configuration, the dashed bond represents either a single or double bond, which double bond may be a cis or trans double bond and R is a saturated or unsaturated acyclic hydrocarbon group having from 1 to 20 carbon atoms, or -(CH2)m R 1 wherein m is 0-10, and R1 is an aliphatic ring having from 3 to 7 carbon atoms, or an aryl or heteroaryl ring having from 4 to 10 carbon atoms and wherein the heteroatom is selected from the group consisting of N, O and S.

11. use for treating intraocular pressure of a compound having EP2 receptor agonist activity.