CA2158570A1 - Opposable-element chromatographic assay device - Google Patents
Opposable-element chromatographic assay deviceInfo
- Publication number
- CA2158570A1 CA2158570A1 CA002158570A CA2158570A CA2158570A1 CA 2158570 A1 CA2158570 A1 CA 2158570A1 CA 002158570 A CA002158570 A CA 002158570A CA 2158570 A CA2158570 A CA 2158570A CA 2158570 A1 CA2158570 A1 CA 2158570A1
- Authority
- CA
- Canada
- Prior art keywords
- opposable
- sample
- components
- variations
- devices
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000007813 chromatographic assay Methods 0.000 title abstract 2
- 238000003556 assay Methods 0.000 abstract 2
- 238000004587 chromatography analysis Methods 0.000 abstract 2
- 239000012501 chromatography medium Substances 0.000 abstract 2
- 238000000605 extraction Methods 0.000 abstract 2
- 238000002360 preparation method Methods 0.000 abstract 2
- 239000012491 analyte Substances 0.000 abstract 1
- 238000003149 assay kit Methods 0.000 abstract 1
- 230000002457 bidirectional effect Effects 0.000 abstract 1
- 230000002860 competitive effect Effects 0.000 abstract 1
- 238000002967 competitive immunoassay Methods 0.000 abstract 1
- 238000003018 immunoassay Methods 0.000 abstract 1
- 238000011065 in-situ storage Methods 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54386—Analytical elements
- G01N33/54387—Immunochromatographic test strips
- G01N33/54388—Immunochromatographic test strips based on lateral flow
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/505—Containers for the purpose of retaining a material to be analysed, e.g. test tubes flexible containers not provided for above
- B01L3/5055—Hinged, e.g. opposable surfaces
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/90—Plate chromatography, e.g. thin layer or paper chromatography
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Pathology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
A chromatographic assay device for use with immunoassays allows rapid and convenient assays of analytes of biological interest, and permits extractions to be carried out in situ avoiding the use of separate extraction vessels. The device has a wide dynamic range and avoids interference from particulates or colored components. In one form, the device comprises: (1) a first opposable component (11) comprising a sample preparation zone adapted to receive a sample to be assayed; and (2) a second opposable component (12) comprising a chromatographic medium (14). The first and second opposable components can be brought into opposition so as to cause the sample preparation zone (13) to apply the sample to be tested to the chromatographic medium. Preferably, the analyte is detected with a visually detectable label. Other variations of the device vary the arrangement of components to provide optimal chromatography for a variety of analyzes, as well as to permit bidirectional chromatography; still other variations are suitable for competitive immunoassays. The devices can be incorporated in test kits, and assay methods, both sandwich and competitive, using the devices are also disclosed.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/040,430 US5877028A (en) | 1991-05-29 | 1993-03-31 | Immunochromatographic assay device |
US040,430 | 1993-03-31 | ||
PCT/US1994/003568 WO1994023300A1 (en) | 1993-03-31 | 1994-03-31 | Opposable-element chromatographic assay device |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2158570A1 true CA2158570A1 (en) | 1994-10-13 |
CA2158570C CA2158570C (en) | 2007-01-23 |
Family
ID=37684127
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002158570A Expired - Fee Related CA2158570C (en) | 1993-03-31 | 1994-03-31 | Opposable-element chromatographic assay device |
Country Status (1)
Country | Link |
---|---|
CA (1) | CA2158570C (en) |
-
1994
- 1994-03-31 CA CA002158570A patent/CA2158570C/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
CA2158570C (en) | 2007-01-23 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
MKLA | Lapsed |
Effective date: 20140402 |