CA2148219A1 - Measurement of Total Molecule in a Sample and Methods Based Thereon - Google Patents

Measurement of Total Molecule in a Sample and Methods Based Thereon

Info

Publication number
CA2148219A1
CA2148219A1 CA2148219A CA2148219A CA2148219A1 CA 2148219 A1 CA2148219 A1 CA 2148219A1 CA 2148219 A CA2148219 A CA 2148219A CA 2148219 A CA2148219 A CA 2148219A CA 2148219 A1 CA2148219 A1 CA 2148219A1
Authority
CA
Canada
Prior art keywords
target molecule
total amount
measurement
molecules
diagnosis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA2148219A
Other languages
French (fr)
Other versions
CA2148219C (en
Inventor
Charles W. Rittershaus
Patrick C. Kung
George H. Parsons
Patricia S. Meisner
Alfred E. Fox
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fujirebio US Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2148219A1 publication Critical patent/CA2148219A1/en
Application granted granted Critical
Publication of CA2148219C publication Critical patent/CA2148219C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56972—White blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/571—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses for venereal disease, e.g. syphilis, gonorrhoea
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6863—Cytokines, i.e. immune system proteins modifying a biological response such as cell growth proliferation or differentiation, e.g. TNF, CNF, GM-CSF, lymphotoxin, MIF or their receptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/106—Pharmacogenomics, i.e. genetic variability in individual responses to drugs and drug metabolism

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Food Science & Technology (AREA)
  • Zoology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Steroid Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The measurement of the total amount of a target molecule is used in the detection, diagnosis, determining the severity of and staging of a variety of diseases or disorders and the monitoring of therapy. The measurements of the total amount of a target molecule is accomplished by treating a sample containing cells, such as a body fluid, with a non-ionic detergent in a manner that makes available for binding to one or more binding partners the total amount of a target molecule present in the cell membrane, the cytoplasm of the cell and/or in the soluble compartments of the body fluid. Once the total target molecule is available, it is measured using any of a number of assay techniques, preferably by sandwich enzyme immunoassay. The methods of the present application may be used to detect and measure molecules associated with microbial pathogens, in particular untracellular pathogens such as Chlamydia, as well as erythrocyte blood group antigens, tumor antigens, cytokines and cell adhesion molecules, for diagnosis, enumeration of cells expressing such molecules, and other methods.
CA002148219A 1992-10-30 1993-10-29 Measurement of total molecule in a sample and methods based thereon Expired - Fee Related CA2148219C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US96879392A 1992-10-30 1992-10-30
US07/968,793 1992-10-30
PCT/US1993/010550 WO1994010571A1 (en) 1992-10-30 1993-10-29 Measurement of total molecule in a sample and methods based thereon

Publications (2)

Publication Number Publication Date
CA2148219A1 true CA2148219A1 (en) 1994-05-11
CA2148219C CA2148219C (en) 1998-12-29

Family

ID=25514787

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002148219A Expired - Fee Related CA2148219C (en) 1992-10-30 1993-10-29 Measurement of total molecule in a sample and methods based thereon

Country Status (5)

Country Link
EP (1) EP0666987A4 (en)
JP (1) JPH07509783A (en)
AU (1) AU686577B2 (en)
CA (1) CA2148219C (en)
WO (1) WO1994010571A1 (en)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2000502452A (en) * 1995-12-22 2000-02-29 ユニバーサル ヘルスウォッチ，インコーポレーテッド Fecal test methods and equipment
US6057166A (en) * 1995-12-22 2000-05-02 Universal Healthwatch, Inc. Fecal test method
US5932430A (en) * 1996-05-09 1999-08-03 Meridian Diagnostics, Inc. Immunoassay for H. pylori in fecal specimens
US5716791A (en) * 1996-05-09 1998-02-10 Meridian Diagnostics, Inc. Immunoassay for H. pylori in fecal specimens
WO2000007021A1 (en) * 1998-07-31 2000-02-10 Mitsubishi Chemical Corporation Method for assaying physiologically active component
JP2001099835A (en) * 1999-09-30 2001-04-13 Internatl Reagents Corp Method for eliminating surface-active agent
EP1234181A4 (en) * 1999-12-03 2005-01-26 Baxter Int Pyrogenicity test for use with automated immunoassay systems
EP1247094A4 (en) * 2000-01-06 2006-10-04 Biosite Diagnostics Inc ASSAYS FOR DETECTION OF i BACILLUS ANTHRACIS /i
DE10043161A1 (en) * 2000-09-01 2002-03-14 Connex Ges Zur Optimierung Von Solution for the preparation of stool samples for diagnostic purposes
ES2350922T3 (en) 2001-09-10 2011-01-28 University Of Pittsburgh Of The Commonwealth System Of Higher Education DIAGNOSIS AND MONITORING OF THE SYSTEMIC ERYTHEMATE LUPUS AND THE SCREDODERMIA.
US7807802B2 (en) 2002-11-12 2010-10-05 Abbott Lab Polynucleotides for the amplification and detection of Chlamydia trachomatis and Neisseria gonorrhoeae
ATE552501T1 (en) 2003-04-16 2012-04-15 Univ Pittsburgh IDENTIFICATION AND MONITORING OF SYSTEMIC LUPUS ERYTHEMATODE
EP2194383A1 (en) 2003-06-13 2010-06-09 University of Pittsburgh Monitoring immunologic, hematologic and inflammatory diseases
DK1756571T3 (en) 2004-04-09 2010-12-13 Univ Pittsburgh Real-time method for detecting acute inflammatory conditions
EP1745287B1 (en) 2004-05-11 2010-09-22 University of Pittsburgh - Of the Commonwealth System of Higher Education Diagnosing and monitoring inflammatory diseases by measuring complement components on white blood cells
KR101352222B1 (en) 2005-12-22 2014-02-04 백스터 인터내셔널 인코포레이티드 Improved monocyte activation test better able to detect non-endotoxin pyrogenic contaminants in medical products
FR2895263B1 (en) * 2005-12-26 2008-05-30 Lab Francais Du Fractionnement CONCENTRATE OF IMMUNOGLOBIN G (LG) DEPLETED ANTI-A AND ANTI-B ANTIBODIES, AND POLYREACTIVE IGG
EP2009111B1 (en) * 2006-03-20 2013-05-29 National University Corporation University of Fukui Non-heating detection method for dermatophyte
JP2010510526A (en) * 2006-11-22 2010-04-02 スリーエム イノベイティブ プロパティズ カンパニー Method for capturing whole bacterial cells and method for analyzing samples for bacteria
JP2010122205A (en) * 2008-08-29 2010-06-03 Sysmex Corp Method for detecting measles virus, membrane assay test device, and membrane assay test kit
EP2333105A1 (en) 2009-12-08 2011-06-15 Koninklijke Philips Electronics N.V. Selective lysis of cells
CN102109525B (en) * 2011-01-26 2013-10-16 牛刚 Kit for detecting free breast cancer cell marker in blood
CN102680678B (en) * 2012-06-08 2014-06-04 北京源德生物医学工程有限公司 Protein eluent for dried blood spots on filter paper
EP2970909A4 (en) * 2013-03-15 2017-02-15 The University of Chicago Methods and compositions related to t-cell activity

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3932222A (en) * 1974-12-20 1976-01-13 J. K. & Susie L. Wadley Research Institute And Blood Bank For isolating pathogenic microorganisms
FR2500164A1 (en) * 1981-02-13 1982-08-20 Fella Christian METHOD FOR THE EVIDENCE OF BLOOD GROUP ANTIGENS A, B, H ON HUMAN CELLS IN A LIQUID MEDIUM
CA2135397A1 (en) * 1992-06-05 1993-12-23 Jean-Michel Dayer T-cell membrane inducing factors

Also Published As

Publication number Publication date
AU686577B2 (en) 1998-02-12
EP0666987A1 (en) 1995-08-16
EP0666987A4 (en) 1997-08-27
JPH07509783A (en) 1995-10-26
AU5457194A (en) 1994-05-24
CA2148219C (en) 1998-12-29
WO1994010571A1 (en) 1994-05-11

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Effective date: 20011029