CA2129172A1 - Thermostable xylanase dna, protein and methods of use - Google Patents

Thermostable xylanase dna, protein and methods of use

Info

Publication number
CA2129172A1
CA2129172A1 CA 2129172 CA2129172A CA2129172A1 CA 2129172 A1 CA2129172 A1 CA 2129172A1 CA 2129172 CA2129172 CA 2129172 CA 2129172 A CA2129172 A CA 2129172A CA 2129172 A1 CA2129172 A1 CA 2129172A1
Authority
CA
Canada
Prior art keywords
protein
methods
thermostable xylanase
high temperature
low
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA 2129172
Other languages
French (fr)
Other versions
CA2129172C (en
Inventor
Ryszard Brzezinski
Claude V. Dery
Carole Beaulieu
Jean Francois Ethier
Serge Harpin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
AB Enzymes GmbH
Original Assignee
Universite de Sherbrooke
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universite de Sherbrooke filed Critical Universite de Sherbrooke
Priority to CA 2129172 priority Critical patent/CA2129172C/en
Publication of CA2129172A1 publication Critical patent/CA2129172A1/en
Application granted granted Critical
Publication of CA2129172C publication Critical patent/CA2129172C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C12N9/2482—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Paper (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention is for a method of chemically treating plant biomass with an enzyme system that retains function at low pH and high temperature. Enzyme preparations enriched in xylanase enzymes which retain activity in low pH and high temperature are described. Such preparations may be utilized in a crude unpurified form, and are especially useful in the production of pulp and paper.
CA 2129172 1994-07-29 1994-07-29 Thermostable xylanase dna, protein and methods of use Expired - Lifetime CA2129172C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA 2129172 CA2129172C (en) 1994-07-29 1994-07-29 Thermostable xylanase dna, protein and methods of use

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CA 2129172 CA2129172C (en) 1994-07-29 1994-07-29 Thermostable xylanase dna, protein and methods of use

Publications (2)

Publication Number Publication Date
CA2129172A1 true CA2129172A1 (en) 1996-01-30
CA2129172C CA2129172C (en) 2002-01-01

Family

ID=4154086

Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2129172 Expired - Lifetime CA2129172C (en) 1994-07-29 1994-07-29 Thermostable xylanase dna, protein and methods of use

Country Status (1)

Country Link
CA (1) CA2129172C (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004083520A1 (en) 2003-03-21 2004-09-30 Council Of Scientific And Industrial Research Process for bio-bleaching of kraft pulp using bacterial consortia
WO2012120184A3 (en) * 2011-03-09 2012-12-27 Cerefi Ltd Separation of lignin from plant material
US9012186B2 (en) 2009-04-27 2015-04-21 The Board Of Trustees Of The University Of Illinois Hemicellulose-degrading enzymes

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004083520A1 (en) 2003-03-21 2004-09-30 Council Of Scientific And Industrial Research Process for bio-bleaching of kraft pulp using bacterial consortia
US9012186B2 (en) 2009-04-27 2015-04-21 The Board Of Trustees Of The University Of Illinois Hemicellulose-degrading enzymes
WO2012120184A3 (en) * 2011-03-09 2012-12-27 Cerefi Ltd Separation of lignin from plant material

Also Published As

Publication number Publication date
CA2129172C (en) 2002-01-01

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Legal Events

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MKEX Expiry

Effective date: 20140729