CA2072891A1 - Proteines de fusion d'exotoxine de pseudomonas, avec modifications carboxyliques pour une cytotoxicite plus grande - Google Patents
Proteines de fusion d'exotoxine de pseudomonas, avec modifications carboxyliques pour une cytotoxicite plus grandeInfo
- Publication number
- CA2072891A1 CA2072891A1 CA2072891A CA2072891A CA2072891A1 CA 2072891 A1 CA2072891 A1 CA 2072891A1 CA 2072891 A CA2072891 A CA 2072891A CA 2072891 A CA2072891 A CA 2072891A CA 2072891 A1 CA2072891 A1 CA 2072891A1
- Authority
- CA
- Canada
- Prior art keywords
- carboxyl
- pseudomonas exotoxin
- alterations
- fusion proteins
- increased cytotoxicity
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
- C07K16/2866—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for cytokines, lymphokines, interferons
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/21—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Pseudomonadaceae (F)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/495—Transforming growth factor [TGF]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/70503—Immunoglobulin superfamily
- C07K14/70514—CD4
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
- C07K2317/77—Internalization into the cell
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/034—Fusion polypeptide containing a localisation/targetting motif containing a motif for targeting to the periplasmic space of Gram negative bacteria as a soluble protein, i.e. signal sequence should be cleaved
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/55—Fusion polypeptide containing a fusion with a toxin, e.g. diphteria toxin
Abstract
Exotoxine de Pseudomonas, cytotoxique, recombinée spécifique à une cible. Lesdites toxines sont obtenues par insertion de molécules de reconnaissance spécifiques sur des sites de clonage spécifiques, dans au moins le domaine III au niveau de la terminaison carboxyle de la molécule PE. Diverses modifications de la terminaison carboxyle de la molécule PE augmentant la cytotoxicité, sont présentées.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US45963590A | 1990-01-02 | 1990-01-02 | |
US459,635 | 1990-01-02 | ||
PCT/US1990/007421 WO1991009949A1 (fr) | 1990-01-02 | 1990-12-27 | Exotoxine de pseudomonase, cytotoxique, recombinee specifique a une cible |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2072891A1 true CA2072891A1 (fr) | 1991-07-03 |
CA2072891C CA2072891C (fr) | 1999-12-21 |
Family
ID=23825594
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002072891A Expired - Fee Related CA2072891C (fr) | 1990-01-02 | 1990-12-27 | Proteines de fusion d'exotoxine de pseudomonas, avec modifications carboxyliques pour une cytotoxicite plus grande |
Country Status (5)
Country | Link |
---|---|
EP (1) | EP0509056A4 (fr) |
JP (1) | JP2610740B2 (fr) |
AU (1) | AU644139B2 (fr) |
CA (1) | CA2072891C (fr) |
WO (1) | WO1991009949A1 (fr) |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU631200B2 (en) * | 1989-02-17 | 1992-11-19 | Merck & Co., Inc. | Production of modified pe40 |
US5458878A (en) * | 1990-01-02 | 1995-10-17 | The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | P. exotoxin fusio proteins have COOHG220101al alterations which increase cytotoxicity |
NZ237758A (en) * | 1990-04-17 | 1992-12-23 | Shell Int Research | (phenylalkyl) triphenylphosphonium salt derivatives and fungicidal compositions |
AU646673B2 (en) * | 1990-05-11 | 1994-03-03 | United States of America, as represented by the Secretary, U.S. Department of Commerce, The | Improved pseudomonas exotoxins of low animal toxicity and high cytocidal activity |
US20070196366A1 (en) | 2003-04-30 | 2007-08-23 | Uwe Zangemeister-Wittke | Methods for treating cancer using an immunotoxin |
US8932586B2 (en) | 2011-09-06 | 2015-01-13 | Intrexon Corporation | Modified forms of Pseudomonas exotoxin A |
WO2016146833A1 (fr) | 2015-03-19 | 2016-09-22 | F. Hoffmann-La Roche Ag | Biomarqueurs de résistance à la nad(+)-diphtamide adp-ribosyltransférase |
EP3184547A1 (fr) | 2015-10-29 | 2017-06-28 | F. Hoffmann-La Roche AG | Anticorps anti-tpbg et procédés d'utilisation |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4545985A (en) * | 1984-01-26 | 1985-10-08 | The United States Of America As Represented By The Secretary, Dept. Of Health And Human Services | Pseudomonas exotoxin conjugate immunotoxins |
US4892827A (en) * | 1986-09-24 | 1990-01-09 | The United States Of America As Represented By The Department Of Health And Human Services | Recombinant pseudomonas exotoxins: construction of an active immunotoxin with low side effects |
US5458878A (en) * | 1990-01-02 | 1995-10-17 | The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | P. exotoxin fusio proteins have COOHG220101al alterations which increase cytotoxicity |
-
1990
- 1990-12-27 EP EP19910904103 patent/EP0509056A4/en not_active Withdrawn
- 1990-12-27 CA CA002072891A patent/CA2072891C/fr not_active Expired - Fee Related
- 1990-12-27 WO PCT/US1990/007421 patent/WO1991009949A1/fr not_active Application Discontinuation
- 1990-12-27 JP JP3504333A patent/JP2610740B2/ja not_active Expired - Lifetime
- 1990-12-27 AU AU72424/91A patent/AU644139B2/en not_active Ceased
Also Published As
Publication number | Publication date |
---|---|
AU644139B2 (en) | 1993-12-02 |
WO1991009949A1 (fr) | 1991-07-11 |
AU7242491A (en) | 1991-07-24 |
EP0509056A4 (en) | 1993-02-17 |
EP0509056A1 (fr) | 1992-10-21 |
JPH05502032A (ja) | 1993-04-15 |
CA2072891C (fr) | 1999-12-21 |
JP2610740B2 (ja) | 1997-05-14 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
MKLA | Lapsed |