CA2002318A1 - Adn encodant des polypeptides¬lys46,asp97,asp113|et¬lys46,asp113,as137p}thaumatine i - Google Patents

Adn encodant des polypeptides¬lys46,asp97,asp113|et¬lys46,asp113,as137p}thaumatine i

Info

Publication number
CA2002318A1
CA2002318A1 CA2002318A CA2002318A CA2002318A1 CA 2002318 A1 CA2002318 A1 CA 2002318A1 CA 2002318 A CA2002318 A CA 2002318A CA 2002318 A CA2002318 A CA 2002318A CA 2002318 A1 CA2002318 A1 CA 2002318A1
Authority
CA
Canada
Prior art keywords
lys46
asp97
thaumatin
polypeptides
amino acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA2002318A
Other languages
English (en)
Other versions
CA2002318C (fr
Inventor
Lindley C. Blair
Raju K. Koduri
Jar-How Lee
Joachim L. Weickmann
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
International Genetic Engineering Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2002318A1 publication Critical patent/CA2002318A1/fr
Application granted granted Critical
Publication of CA2002318C publication Critical patent/CA2002318C/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C07K14/43—Sweetening agents, e.g. thaumatin, monellin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
CA002002318A 1988-11-08 1989-11-06 Adn encodant des polypeptides¬lys46,asp97,asp113|et¬lys46,asp113,as137p}thaumatine i Expired - Fee Related CA2002318C (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US26870288A 1988-11-08 1988-11-08
US268,702 1988-11-08
US407,416 1989-09-14
US07/407,416 US5221624A (en) 1988-11-08 1989-09-14 DNA encoding (Lys46, Asp97, Asp113) and (Lys46, Asp.sup.137) thaumatin I polypeptides

Publications (2)

Publication Number Publication Date
CA2002318A1 true CA2002318A1 (fr) 1990-05-08
CA2002318C CA2002318C (fr) 2000-05-23

Family

ID=26953274

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002002318A Expired - Fee Related CA2002318C (fr) 1988-11-08 1989-11-06 Adn encodant des polypeptides¬lys46,asp97,asp113|et¬lys46,asp113,as137p}thaumatine i

Country Status (8)

Country Link
US (1) US5221624A (fr)
EP (1) EP0396741B1 (fr)
JP (1) JPH03502645A (fr)
AT (1) ATE142687T1 (fr)
AU (2) AU625879B2 (fr)
CA (1) CA2002318C (fr)
DE (1) DE68927167T2 (fr)
WO (1) WO1990005775A1 (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5234834A (en) * 1987-06-19 1993-08-10 The Regents Of The University Of California Constructs for expression of monellin in plant cells
ES2080689B1 (es) * 1994-04-21 1996-09-01 Urquima Sa Procedimiento de obtencion de un edulcorante natural proteico.
AU6674296A (en) * 1995-06-23 1997-01-22 University Of Hawaii Recombinant sweet protein mabinlin
GB9724307D0 (en) * 1997-11-17 1998-01-14 Zeneca Ltd Improvements relating to protein accumulation
US6274707B1 (en) 1999-04-14 2001-08-14 Wisconsin Alumni Research Foundation Protein sweetener
US6420527B1 (en) * 1999-05-06 2002-07-16 International Flavors & Fragrances Inc. Flavor active modified thaumatin and monellin and methods for their production and use
US9101160B2 (en) 2005-11-23 2015-08-11 The Coca-Cola Company Condiments with high-potency sweetener
US8017168B2 (en) 2006-11-02 2011-09-13 The Coca-Cola Company High-potency sweetener composition with rubisco protein, rubiscolin, rubiscolin derivatives, ace inhibitory peptides, and combinations thereof, and compositions sweetened therewith
KR101083040B1 (ko) 2010-02-24 2011-11-16 중앙대학교 산학협력단 높은 단맛을 가지는 신규한 브라제인 다중 변이체 및 이의 제조방법
WO2011105841A2 (fr) * 2010-02-24 2011-09-01 중앙대학교 산학협력단 Vecteur d'expression recombinant pour exprimer la brazzéine, et variante de brazzéine à mutation multiple dont la douceur est supérieure

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3177281T2 (de) * 1980-12-12 1992-12-10 Unilever Nv Strukturgene, die fuer die verschiedenen allelischen und reifungsformen von praeprothaumatin und deren mutanten kodieren, diese strukturgene enthaltende klonierungsvektoren und deren expression in mikrobiellen wirtzellen.
HU204097B (en) * 1982-05-19 1991-11-28 Gist Brocades Nv Process for producing cloning system relating to kluyveromyces species
US4546082A (en) * 1982-06-17 1985-10-08 Regents Of The Univ. Of California E. coli/Saccharomyces cerevisiae plasmid cloning vector containing the alpha-factor gene for secretion and processing of hybrid proteins
AU577334B2 (en) * 1983-10-11 1988-09-22 International Genetic Engineering, Inc. Synthetic thaumatin genes
CA1310923C (fr) * 1985-11-13 1992-12-01 Joachim Ludwig Weickmann Adn encodant la [asp --]- et la [lys -, asp --] -thaumatine i
AU621277B2 (en) * 1985-11-13 1992-03-12 Xoma Corporation Shortened phosphoglycerate kinase promoter
US4766205A (en) * 1985-11-13 1988-08-23 Beatrice Companies, Inc. Method for isolation of recombinant polypeptides in biologically active forms
IL82935A0 (en) * 1986-08-12 1987-12-20 Phillips Petroleum Co Secretion of heterologous proteins from yeast

Also Published As

Publication number Publication date
JPH03502645A (ja) 1991-06-20
AU5045090A (en) 1990-06-12
AU2707092A (en) 1992-12-17
AU625879B2 (en) 1992-07-16
ATE142687T1 (de) 1996-09-15
CA2002318C (fr) 2000-05-23
WO1990005775A1 (fr) 1990-05-31
US5221624A (en) 1993-06-22
AU651501B2 (en) 1994-07-21
EP0396741A1 (fr) 1990-11-14
EP0396741A4 (en) 1992-03-11
DE68927167T2 (de) 1997-04-03
DE68927167D1 (de) 1996-10-17
EP0396741B1 (fr) 1996-09-11

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Legal Events

Date Code Title Description
EEER Examination request
MKLA Lapsed