CA1333893C - Enzymatic hydrolysis of beef tallow - Google Patents
Enzymatic hydrolysis of beef tallowInfo
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- CA1333893C CA1333893C CA000546989A CA546989A CA1333893C CA 1333893 C CA1333893 C CA 1333893C CA 000546989 A CA000546989 A CA 000546989A CA 546989 A CA546989 A CA 546989A CA 1333893 C CA1333893 C CA 1333893C
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- lipase
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- fatty acids
- oil
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6409—Fatty acids
- C12P7/6418—Fatty acids by hydrolysis of fatty acid esters
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11C—FATTY ACIDS FROM FATS, OILS OR WAXES; CANDLES; FATS, OILS OR FATTY ACIDS BY CHEMICAL MODIFICATION OF FATS, OILS, OR FATTY ACIDS OBTAINED THEREFROM
- C11C1/00—Preparation of fatty acids from fats, fatty oils, or waxes; Refining the fatty acids
- C11C1/02—Preparation of fatty acids from fats, fatty oils, or waxes; Refining the fatty acids from fats or fatty oils
- C11C1/04—Preparation of fatty acids from fats, fatty oils, or waxes; Refining the fatty acids from fats or fatty oils by hydrolysis
- C11C1/045—Preparation of fatty acids from fats, fatty oils, or waxes; Refining the fatty acids from fats or fatty oils by hydrolysis using enzymes or microorganisms, living or dead
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D13/00—Making of soap or soap solutions in general; Apparatus therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
- C12N9/20—Triglyceride splitting, e.g. by means of lipase
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- Oil, Petroleum & Natural Gas (AREA)
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- General Engineering & Computer Science (AREA)
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Abstract
An enzymatic process of hydrolyzing fats, particularly high melting fats such as high melting beef tallow, with a non-stereospecific animal or vegetable lipase, parti-cularly the castor bean lipase, in the presence of about 10-25% by weight of a vegetable oil, such as coconut oil, as the emulsifying agent, at an acidic pH of about 4-5.5 and at a temperature not to exceed 50°C; and recovering a high yield of fatty acids for use in the production of soap.
Description
~.33389~, Background of the Invention and Prior Art This invention relates to the proce~s of converting high melting fats, such as high grade beef tallow, into a hi8h yield of fatty acids and glycerol, comprising the hydrolysis of beef tallow in the presence of a minor amount of coconut oil by means of the castor bean lipase enzyme, at low temperatures, of about 25-50C, and preferably about 37C, for use in the produc-i tion of soap. The high yield of fatty acids (98% conversion to fatty acids and glycerol), and the utilization of low temperatures in this process is accomplished by the addition of a minor amount of coconut oil or other vegetable oil, which permits the emulsi-fication of the beef tallow at lower temperatures, (37C). This process eliminates undesirable thermal products and provides a savings in energy without resorting to undesirable additives.
The fatty acid and glycerol mixture is free of undesirable extraneous materials, because the coconut oil additive also hydrolyzes into fatty acids and glycerol.
Soap manufacturing is usually accompliahed by saponifi-cation of high grade beef tallow with lye. This is a high temperature reaction which has become expensive in recent year due to the sharp rise in fuel costs. Hence, a low temperature reaction was sought, and enzymatic hydrolysis with lipases was investigated, as a substitute for the lye process.
The optimum conditions of lipase reactions is usually as an emulsion at 37C. Unfortunately, the problem is that fats such a~ high grade beef tallow, do not start melting until at least 41C to 50C. Therefore, they do not form emulsions at 37C in water without additives. It is apparently for this -2- ~
~_ !
1 33389~
reason that in systems containing only beef tallow, water, and a lipase preparation, the yields of fatty acids are always low or not-reported. Moskowitz et al, J. Agric. Food Chem., 25 1146 (1977). Constantin et al, Biochim. et Biophys. Acta, 43, 103 (1960). Ralston, Fatty Acids and Their Derivatives, pp. 274-279, Wiley (1948). Haley et al, J. Am. Chem. Soc., 43, 2664 (1921).
Raising the temperature of the lipase retction did not solve this problem, because of the rapid loss of stability of lipases at elevated temperatures. For example, pancreatic lipase loses 36% of its activity after 10 minutes at 50C
because enzymes are denatured at raised temperatures.
In an attempt to overcome this problem, the reaction system was modified by the addition of metallic additives such as calcium and magnesium salts, which did give higher yields, as disclosed in Constantin et al (supra); Altschul et al, Federation Proc., 18, 180 (1959); Kokusho et al, Jpn Kokai Tokkyo Koho 79, 95, 607 (1979); Benzonana et al, Biochim. Biophys. Acta, 164, 47 (1968). However, the metal soips formed in this reaction are hard soaps which provide unsatisfactory foaming and cleansing action. Haley et al (tupra) added petroleum ether as a fat solvent in order to get better physical contact between eneyme and substrate (beef tallow) to increase or accelerate hydrolysis. This process how-ever is one of considerable danger due to the high explosion potential of the solvent.
Since solid fats are very difficult to emulsify, a study on the selection of emulsifiers of natural (solid) fats was made by Lobreva, et al, 1333~3 Micro -biologiya, 48, 53 (1979) in an attempt to increase lipoly-tic activity on fats. The emulsifying agents disclosed herein for the lipolysis of animal fats such a~ lard, beef and lamb fats ; ! #~
are-Triton X-100, Triton X-305, egg albumen , gelatin, gum arabic, lecithin , and Tween-60. Not all of these agents were successful in emulsifying beef tallow. This lipolytic reaction did not give a high yield of fatty acids and glycerol.
Furthermore, said emulsifying agents have the disadvantage of providing undesirable materials to the hydrolysis mixture.
The use of the castor bean lipase in the hydrolysis of fats, and its preparation, are well known in the art, as dis-closed in U.S. Patent No. 2,485,779, wherein a solvent extracted, ground castor bean meal, prepared at A temperature not exceeding 120F is used in the partial hydrolysis of fish oil. Diethyl ether extracted, ground ca~tor seed kernels have been used in the hydrolysis of low quality industrial fats, as disclosed in Trosko et al, Maslo-Zhir, Prom-st, 1977, 27; and in the hydrolysis of sunflower oil, as disclosed in Meerow et al, Prikl. Biokhem.
Mikrobiol., 12 934 (1976). Castor bean lipase prepared by cen-trifuging a homogenate of the kernels into a fatty layer which is extracted by ether in the pre~ence of a saturated salt solution has been used in the hydrolysis of cottonseed oil, mono- and diolein, castor oil and a cottonseed oil emulsion, as disclosed in Altschul et al, (supra)l Haley et al, (supra) discloses a method of preparing a castor bean lipase by extracting the hull-free kernels with petroleum ether hydrolysis of fats and oil. Ralston, Fatty Acids and Their Derivative, Wiley, p. 276 (1948) disclose~ variations in the Jf~c~ 4-method of preparing an active lipase from castor beans for use in the hydrolysis of fat containing 40-50% water, and in the ! presence of a small amount of acetic acid or an activating salt such as manganese sulfate. The general method includes grinding the dehulled seeds in water, filtering the solids, and centrifuging to form an emulsion. One variation thereof is grinding the dry seeds in cottonseed oil and centrifuging the ~ixture.
Another method includes extracting the macerated beans with petroleum ether, drying, pulverizing and sifting the product, which retains its original activity, over a period of ten years.
However, there is no disclosure of the low temperature hydrolysis of high melting point fats such as beef tallow with a non-stereospecific animal or vegetable lipase, particularly the castor bean lipase, in the presence of a vegetable oil emulsifying agent such as coconut oil at an acidic pH and a low temperature under 50C.
Summary of the Invention It has now been found that the low temperature enzymatic hydrolysis of high melting fats such as beef tallow, utilizing a non-stereospecific animal or vegetable lipase enzyme, such as castor bean lipase, and a vegetable oil emulsifying agent such as coconut oil provides the almost quantative hydrolysis of the mixture of said beef tallow and coconut oil into fatty acids and glycerol free of extraneous undesirable materials.
Neutralization with sodium hydroxide forms a high grade soap (i.e. free of contaminants).
13~8~3 62301-1448 Accordingly, it is an aim of the present invention to provide a process of hydrolyzing high melting fats, such as high grade beef tallow, into a high yield of fatty acids and glycerol, at a low temperature, with a non-stereospecific lipase such as castor bean lipase, in the presence of a vegetable oil emulsifying agent.
Another aim of thi~ invention is to provide a process of converting high melting beef tallow into fatty acids and glycerol, ln high yield, at low temperatures by hydrolyzing a mixture of beef tallow and a minor amount of coconut oil with the castor bean lipase enzyme.
Still another aim of this invention is to provlde an enzymatic hydrolysis process of converting a mixture of a high melting fat and a low melting vegetable oil into a reaction mixture of fatty acids and glycerol free of undesirable minerals, i.e. thermal products and addltlves.
Another aim of this invention is to provide an enzyme system to hydrolyze beef tallow and coconut oil into fatty acids ~and glycerol) and neutrallzing said fatty acids wlth NaOH, Na2C03 or NaHCO3 to form soap.
Additional advantages will be apparent from a consideration of the following description and ln part wlll become apparent to those skilled in the art upon examination of the following or may be learned by practice of the lnventlon.
The objects and advantages of the invention may be realized and attained by mean~ of the instrumentalities and combinations particularly pointed out in the appended claims.
To achieve the foregoing in accordance with the present invention, as embodied and broadly described herein, the process of thls invention of converting high melting fats .~ 6 -13~38~3 into fatty acids and glycerol comprises hydrolyzlng an emulsified mixture of about 90-75% by weight of a high meltlng beef tallow and about 10-25% by weight of the mixture of a vegetable oil, preferably coconut oll, in an aqueous medium, with a non-stereospecific animal or vegetable lipase enzyme, at a temperature of about 25-50C, preferably 37C, and at a pH of about 4-5.5, and recovering a final reaction mixture consisting of fatty acids, glycerol and lipase. The mixture is agitated for a sufficient period of time, about 2 to 48 hours, to obtain substantially complete hydrolysis into fatty aclds and glycerol. The final reaction mixture is free of undesirable materials, and consists of three layers, a fatty acid top layer, a lipase mixture middle layer, and an aqueous glycerin (sweet water) bottom layer. The layers may be separated. The fatty acid layer is skimmed off, neutralized with NaOH, Na2C03, or NaHCO3, and the resulting soap purified in the usual manner of soap manufacturers skilled in the art. The bottom layer is separated, and the glycerine removed. The middle layer, containing more than 50% of the original lipase still being active, is reused after adding a lesser quantity of fresh lipase.
The present lnvention also relates to a process of producing a high grade soap, free of undesirable additlves, which comprises hydrolyzing an emulsified mixture of a high melting fat and about 10-25~ by weight of a vegetable oil in an aqueous medium, with a non-stereospecific animal or vegetable lipase at a temperature of about 25-50C and at a pH of about 4-5.5, agitating the mixture for a period of time to obtain substantially complete hydrolysis into fatty acids and glycerol separating the fatty acids from the glycerol, and neutralizing ~ 333~3 said fatty acids with an alkaline material to form a soap, free of undesirable additives. More specifically, the final reac-tion mixture consi~ts of three layers, a fatty ncid top layer, a lipase mixture middle layer and an aqueous glycerin bottom layer,andtheprocessincludesseparatingthetoplayeroffattyacidfromthefi~
reaction mixture, and neutralizing said fatty acids with an alkaline material selected from the group consisting of sodium hydroxide, sodium carbonate and sodium bicarbonate to form a sodium soap substantially free of contaminants. The resulting soap and glycerine is much lighter in color than the corresponding colors after sulfuric acid hydrolysis of fats or the high temperature sodium hydroxide saponification of fats.
More specifically, present invention relates to a process of hydrolyzing a high melting fat into a high yield of fatty acids for use in the production of soap free of under-sirable additives, which comprises reacting a mixture of about 90-75% of a high melting beef tallow and about 10-25% coconut oil, with castor bean lipase, in about 20-50% water acidified to ~ r~c a~
a pH of about 4-5.5 with a weak acid such as acetic acid, ~
phosphoric acid, phosphorous acid and carbonic acid, and at a temperature of about 37C, agitating the reaction mixture for about 3-48 hours, to obtain a final reaction mixture con-sisting of three layers, a fatty acid top layer, a lipase mixture in the middle layer and an aqueous glycerin bottom layer, separ-ating the layers and neutralizing said fatty acids to form soap.
The middle layer which contains active castor bean lipase is reused in~,the hydrolyzation process.
-The llpase enzyme used as a catalyst in the present hydrolysis process may be any animal or vegetable llpase which is non-stereospecific, i.e. the lipase must split the beta (middle carboxyl linkage) glycerlde linkage at about the same rate as splittlng the alpha (outer carboxyl) llnkages.
Suitable examples of non-stereospecific lipase enzymes are derived from castor bean, Candida cylindracea, Propionibacterium acnes, Rhizopus arrhizus, Staphylococcus aureus, Aspergillus flavus and Geotrichum candidum. Most lipases are stereospecific and therefore are ineffective, e.g., porcine pancreatic lipase. Normally the extent of hydrolysis with these enzymes does not exceed 70%, whereas the non-stereospecific enzymes affect substantially complete hydrolysis.
The preferred lipase enzyme utillzed herein is the castor bean lipase (ricinus communis). It is lnsoluble in water, and its activity is materially reduced by contact with water. The enzyme is stabilized by the presence of fats. It is rapidly inactivated by alkalls and functions only in a neutral or slightly acidic medium. This enzyme is activated by the presence of acids, preferably weak acids, such as acetic acid, phosphoric acid, phosphorous acld, carbonlc acid, etc., which exerts the greatest accelerating effect. Accordingly, the optimum temperature for ricinus lipase action is about 37C, and is inactivated at temperatures above about 50C. The amount of lipase used in the hydrolysis process is about 3-15%
of the substrate (fat and oil) by weight, if the lipase activity (LA) is unknown. If the LA is known, approximately one LA unit is used for every 10 microequivalents of potential acid. (See N. Pelch and M. C. Kranz, Anal Biochem, 112, 219-222 ~1981) for a procedure for determination of llpase activity).
~3338~3 An additional advantage in the use of the castor bean lipase in the hydrolysi~ reaction is the ability to recover (~eparate) said lipase from the final reaction mixture and recycle it for use with a fresh substrate (fat and oil). This capability results from the natural immobilization property of ca~.tor bean 1 ipas e .
The castor bean lipase cannot be obtained commercially, but can be prepared as disclosed in the prior art previously discussed. The castor bean lipase utilized herein is prepared by dehulling castor beans, extracting the ~ oils by grinding the dehulled beans in the presence of lowboiling petroleum ether, f ilt ering the ground bean pcnace and discarding the filtrate, i.e.
ether layer containing endogenous oils, repeating the eXtraction and filtration ~teps two more times, air drying the filtered pomace and recovering a lipase preparation in the form of the pomace. Another method of preparing the castor bean lipase enzyme without allergen may also be used. This method of pre-paration should be considered because of the pre~enc e of a potent allergen in the bean. The dehulled bean is macerated in water, rather than petroleum ether and then centrifuged.
The fat layer is separated from the aqueous layer, and the aqueous layer is discarded. Since the lipase is in the spherosome~ along with the endogenous oil, it will remain in the fat layer. The fat layer is extracted with petroleum ether and saturated NAC1 solution. The petroleum ether contain~ the endogenous oil, and is discarded. The saturated NaCl solution contains the lipase in particulate matter.
~ 333893 The optimum condition~ for activity of castor bean lipase i5 about 25-50C and preferably about 37 and a pH of 4 to 5.5. A dilute acid such as O.lN acetlc acid, or other weak acids may be added to bring the pH to approximately 5Ø
The amount of the castor bean lipase enzyme used in the present hydrolysis process should be sufficient to effect a substantial degree of conversion of the beef tallow/coconut oil mixture into fatty acids and glycerol. Amounts of about 3 to 15% and preferably about 10% by weight of thetriglyceride substrate mixture is used.
It has unexpectedly been found that the addition of a vegetable oil such as coconut, corn, soybean, lin~eed, olive and palm oil, to the high melting point beef tallow enables the beef tallow to emulsify at a lower temperature, about 37C. The lipase enzyme hydrolyzes the emulsified tallow/vegetsble oil mixture almost quantitatively at the lower temperature with a resultant savings in energy. The vegetable oil such as coconut oil also hydrolyze~ into fatty acids and glycerol. ThuA, no undesirable extraneous ma~erial~ are present in the reaction mixture, j thereby yielding a substantially pure soap upon neutralization of the fatty acids with sodium hydroxide, sodium carbonate or sodium bicarbonate. The coconut oil constitutes a lesser amount by weight than the beef tallow in a mixture thereof.
The weight ratio of beef tallow to vegetable oil is about 75-90% to about 25-10% vegetable oil.
133389 ~
The data in Table I show that a mixture of high grade edible beef tallow and coconut oil, at the same ratio as may be used in soaps, was hydrolyzed approximately 98~ into fatty acids at the described conditions. This is in clear contrast to results reported in the literature (~eyet ~, ~), ~ ~ch ~fta~ ~ reacted ~th ~tor ~3~ ~e ~t ~ o~yhy~olyz~ d~OUt 3% (Table II). In the latter case, coconut oil was absent.
The combined results clearly show that the addition of coconut oil unexpectedly yields a high conversion of the high melting beef tallow into fatty acids. It is believed that the coconut oil lowered the melting point of the beef tallow enough so that it would form an emulsion at 37C-Table I
:;
Extent of Castor Bean Lipase Hydrolysis of Fats and Oils Observed I Lit, S.V.* Enzyme Hydrolysis Value ; Olive oil (control 186-196 185 83:17 Tallow:coco 203-213 203 j *Saponification Value is the weight (mg) of KOH required to I saponify lg of fat, and is indicative of the extent of hydrolysis of the fats and oils, Table II
Hydrolysis of Beef Tallow by Castor Bean Lipase Duration of Experiment (hrs.) Extent of Hydrolysis (%) 24 2.8 48 2.8 72 2.8 The saponification value is determined by conventional methods described in the literature, M. Applewhite, Kirk-Othmr. Encycl. Chem. Tech., 3rd Ed., 9, 795 (1980). The saponification procedure utilized herein comprises adding 30 ml of absolute ethanol to a 2 to 3g sample in a covered flask, warming on a ~team bath (50-60C), adding 50 ml standardized 0.5N alcoholic KOH solution and boiling for one hour, adding phenolphthalein indicator solution a~d titrating with standardized 0.5N HCl to the disappearance of the pink color, and the ~aponification value is determined.
Saponification of fats and oils in the absence of lipase, to determine the maximum yield of soap availab~e from fats and oils, wa~ conducted on four substrates. Triolein and olive oil are standards used in literature. Beef tallow and coconut oil are components of the soaps prepared herein. The data in Table III shows that the observed results are in fairly good agreement with the literature values for the two standards and coconut oil, but low for beef tallow.
Table III
Saponification Values (SV) Lit Obs.
Triolein 189 (calc) 190 Olive oil 186-196 182 Beef tallow 193-202 173 Coconut oil 250-264 253 .
13338~3 :1, i' The advantages of the enzymatic hydrolysis process of present invention are multifaceted. Higher yields of fatty acids and glycerol are obtained, about 98% conversion. The use of lower temperatures resulted in considerable cost savings.
The use of vegetable oil such as coconut oil which also hydro-lyzes into fatty acids a~d glycerol yields fatty acids and glycerol free of undesirable additives. Ssid fatty acids yield a pure soap upon nuetralization with sodium hydroxide, carbonate, ' or bicarbonate. This low temperature hydrolysis reaction requires less utilization of energy, and therefore, less atmos-pheric pollution i~ produced. The use of a low reaction tem-perature in this process yields fewer undesirable thermal pro-ducts, and a lighter color.
Detailed Descriptioniofithe Invention The following examples are merely illustrative of the invention and are not to be construed as limiting thereof.
Example 1 Preparation of Enzyme Dehull 60g of castor beans. Grind the dehulled beans in a Waring blender in the presence of 600ml of low boiling j (30-60C) petroleum ether. The ether extracts endogenous oils from the beans. Filter and discard filtrate. Repeat extraction and filtration two more timesi. Air dry the crude lipase prepara-tion.
Enzymatic Hydrolysis of Tallow/Coconut Mixture Mix 1.67g of the lipase preparation snd 16.7g of an 83:17 mixture of high grade beef tallow and coconut oil, and 10 cc of O.lN acetic acid. Stir approximately 24 hoursi at 37C. The final mixture consists of 3 layers, fatty acid on the top, lipase mixture in the middle, and sweet water (aqueous glycerine) on the bottom. The fatty acid layer is skimmed off, neutralized with NaOH, Na2CO3, or NaHCO3, and the resulting soap purified in the usual manner of soap manufacturers skilled in the art. The bottom layer is separated, and th~ glycerine removed.
The middle layer, containing more than 50% of the original lipa~e still being active, ls reused after adding a lesser quantity of fresh lipaise.
Analysis of the extent of hydrolysis is determined by adding 3.4 g of reaction mixture to 100 ml absolute alcohol, and tritrating to pH 9.5 with O.lN alcoholic potassiium hydroxide.
Results: 98% conversion to fatty acids and glycerol.
~3~3~93 Example 2 ~reparation of Castor Bean Lipase 60g of dehulled castor beans is macerated in water and centrifuged. The fat layer is separated from the aqueous layer, which is discarded. The fat layer is extracted with 600 ml low boiling petroleum ether (30-60C) and saturated NaCl ; solution. The petroleum ether is discarded, and the NaCl r solution contains the lipase in particulate form. 1.67 g of this particulate lipase is used in the hydrolysis of the tallow/
coconut mixture in accordance with the proces~ of Example 1.
Substan~ially complete hydrolysis into fatty acids and glycerol is obtained.
Example 3 The high melting beef tallow is melted at a temperature of about 42C until it is liquified. The liquified tallow is mixed with the coconut oil in the weight ratio of 80:20 tallow:oil.
1.67g of the lipase preparation of Example 1 is mixed with 16.7 g of the liquified fat and oil mixture, and 10 cc of O.lN acetic acid for about 24 hours at 37C. Substantially complete hydroly~is into fatty acids and glycerol is obtained.
.
i i ~ 3~389~-Other weak acids may be substituted for the acetic acid in tne examples, such as phosphoric, phosphorous or carbonic acid.
Lower melting point fats such as sheep tallow, industrial quality beef tallow, lard and butter may be used in the present lipase hydrolysis process.
Also, other vegetable oils such as corn, soybean, linseed, olive and palm oil may be substituted for the coconut oil in the example It is understood that the foregoing detailed description is given merely by way of illustration and that variations may be made therein without departing from the spirit of the invention. The "Abstract" given above is merely for the convenience of technical searchers and is not to be given any weight with respect to the scope of the inventlon.
The fatty acid and glycerol mixture is free of undesirable extraneous materials, because the coconut oil additive also hydrolyzes into fatty acids and glycerol.
Soap manufacturing is usually accompliahed by saponifi-cation of high grade beef tallow with lye. This is a high temperature reaction which has become expensive in recent year due to the sharp rise in fuel costs. Hence, a low temperature reaction was sought, and enzymatic hydrolysis with lipases was investigated, as a substitute for the lye process.
The optimum conditions of lipase reactions is usually as an emulsion at 37C. Unfortunately, the problem is that fats such a~ high grade beef tallow, do not start melting until at least 41C to 50C. Therefore, they do not form emulsions at 37C in water without additives. It is apparently for this -2- ~
~_ !
1 33389~
reason that in systems containing only beef tallow, water, and a lipase preparation, the yields of fatty acids are always low or not-reported. Moskowitz et al, J. Agric. Food Chem., 25 1146 (1977). Constantin et al, Biochim. et Biophys. Acta, 43, 103 (1960). Ralston, Fatty Acids and Their Derivatives, pp. 274-279, Wiley (1948). Haley et al, J. Am. Chem. Soc., 43, 2664 (1921).
Raising the temperature of the lipase retction did not solve this problem, because of the rapid loss of stability of lipases at elevated temperatures. For example, pancreatic lipase loses 36% of its activity after 10 minutes at 50C
because enzymes are denatured at raised temperatures.
In an attempt to overcome this problem, the reaction system was modified by the addition of metallic additives such as calcium and magnesium salts, which did give higher yields, as disclosed in Constantin et al (supra); Altschul et al, Federation Proc., 18, 180 (1959); Kokusho et al, Jpn Kokai Tokkyo Koho 79, 95, 607 (1979); Benzonana et al, Biochim. Biophys. Acta, 164, 47 (1968). However, the metal soips formed in this reaction are hard soaps which provide unsatisfactory foaming and cleansing action. Haley et al (tupra) added petroleum ether as a fat solvent in order to get better physical contact between eneyme and substrate (beef tallow) to increase or accelerate hydrolysis. This process how-ever is one of considerable danger due to the high explosion potential of the solvent.
Since solid fats are very difficult to emulsify, a study on the selection of emulsifiers of natural (solid) fats was made by Lobreva, et al, 1333~3 Micro -biologiya, 48, 53 (1979) in an attempt to increase lipoly-tic activity on fats. The emulsifying agents disclosed herein for the lipolysis of animal fats such a~ lard, beef and lamb fats ; ! #~
are-Triton X-100, Triton X-305, egg albumen , gelatin, gum arabic, lecithin , and Tween-60. Not all of these agents were successful in emulsifying beef tallow. This lipolytic reaction did not give a high yield of fatty acids and glycerol.
Furthermore, said emulsifying agents have the disadvantage of providing undesirable materials to the hydrolysis mixture.
The use of the castor bean lipase in the hydrolysis of fats, and its preparation, are well known in the art, as dis-closed in U.S. Patent No. 2,485,779, wherein a solvent extracted, ground castor bean meal, prepared at A temperature not exceeding 120F is used in the partial hydrolysis of fish oil. Diethyl ether extracted, ground ca~tor seed kernels have been used in the hydrolysis of low quality industrial fats, as disclosed in Trosko et al, Maslo-Zhir, Prom-st, 1977, 27; and in the hydrolysis of sunflower oil, as disclosed in Meerow et al, Prikl. Biokhem.
Mikrobiol., 12 934 (1976). Castor bean lipase prepared by cen-trifuging a homogenate of the kernels into a fatty layer which is extracted by ether in the pre~ence of a saturated salt solution has been used in the hydrolysis of cottonseed oil, mono- and diolein, castor oil and a cottonseed oil emulsion, as disclosed in Altschul et al, (supra)l Haley et al, (supra) discloses a method of preparing a castor bean lipase by extracting the hull-free kernels with petroleum ether hydrolysis of fats and oil. Ralston, Fatty Acids and Their Derivative, Wiley, p. 276 (1948) disclose~ variations in the Jf~c~ 4-method of preparing an active lipase from castor beans for use in the hydrolysis of fat containing 40-50% water, and in the ! presence of a small amount of acetic acid or an activating salt such as manganese sulfate. The general method includes grinding the dehulled seeds in water, filtering the solids, and centrifuging to form an emulsion. One variation thereof is grinding the dry seeds in cottonseed oil and centrifuging the ~ixture.
Another method includes extracting the macerated beans with petroleum ether, drying, pulverizing and sifting the product, which retains its original activity, over a period of ten years.
However, there is no disclosure of the low temperature hydrolysis of high melting point fats such as beef tallow with a non-stereospecific animal or vegetable lipase, particularly the castor bean lipase, in the presence of a vegetable oil emulsifying agent such as coconut oil at an acidic pH and a low temperature under 50C.
Summary of the Invention It has now been found that the low temperature enzymatic hydrolysis of high melting fats such as beef tallow, utilizing a non-stereospecific animal or vegetable lipase enzyme, such as castor bean lipase, and a vegetable oil emulsifying agent such as coconut oil provides the almost quantative hydrolysis of the mixture of said beef tallow and coconut oil into fatty acids and glycerol free of extraneous undesirable materials.
Neutralization with sodium hydroxide forms a high grade soap (i.e. free of contaminants).
13~8~3 62301-1448 Accordingly, it is an aim of the present invention to provide a process of hydrolyzing high melting fats, such as high grade beef tallow, into a high yield of fatty acids and glycerol, at a low temperature, with a non-stereospecific lipase such as castor bean lipase, in the presence of a vegetable oil emulsifying agent.
Another aim of thi~ invention is to provide a process of converting high melting beef tallow into fatty acids and glycerol, ln high yield, at low temperatures by hydrolyzing a mixture of beef tallow and a minor amount of coconut oil with the castor bean lipase enzyme.
Still another aim of this invention is to provlde an enzymatic hydrolysis process of converting a mixture of a high melting fat and a low melting vegetable oil into a reaction mixture of fatty acids and glycerol free of undesirable minerals, i.e. thermal products and addltlves.
Another aim of this invention is to provide an enzyme system to hydrolyze beef tallow and coconut oil into fatty acids ~and glycerol) and neutrallzing said fatty acids wlth NaOH, Na2C03 or NaHCO3 to form soap.
Additional advantages will be apparent from a consideration of the following description and ln part wlll become apparent to those skilled in the art upon examination of the following or may be learned by practice of the lnventlon.
The objects and advantages of the invention may be realized and attained by mean~ of the instrumentalities and combinations particularly pointed out in the appended claims.
To achieve the foregoing in accordance with the present invention, as embodied and broadly described herein, the process of thls invention of converting high melting fats .~ 6 -13~38~3 into fatty acids and glycerol comprises hydrolyzlng an emulsified mixture of about 90-75% by weight of a high meltlng beef tallow and about 10-25% by weight of the mixture of a vegetable oil, preferably coconut oll, in an aqueous medium, with a non-stereospecific animal or vegetable lipase enzyme, at a temperature of about 25-50C, preferably 37C, and at a pH of about 4-5.5, and recovering a final reaction mixture consisting of fatty acids, glycerol and lipase. The mixture is agitated for a sufficient period of time, about 2 to 48 hours, to obtain substantially complete hydrolysis into fatty aclds and glycerol. The final reaction mixture is free of undesirable materials, and consists of three layers, a fatty acid top layer, a lipase mixture middle layer, and an aqueous glycerin (sweet water) bottom layer. The layers may be separated. The fatty acid layer is skimmed off, neutralized with NaOH, Na2C03, or NaHCO3, and the resulting soap purified in the usual manner of soap manufacturers skilled in the art. The bottom layer is separated, and the glycerine removed. The middle layer, containing more than 50% of the original lipase still being active, is reused after adding a lesser quantity of fresh lipase.
The present lnvention also relates to a process of producing a high grade soap, free of undesirable additlves, which comprises hydrolyzing an emulsified mixture of a high melting fat and about 10-25~ by weight of a vegetable oil in an aqueous medium, with a non-stereospecific animal or vegetable lipase at a temperature of about 25-50C and at a pH of about 4-5.5, agitating the mixture for a period of time to obtain substantially complete hydrolysis into fatty acids and glycerol separating the fatty acids from the glycerol, and neutralizing ~ 333~3 said fatty acids with an alkaline material to form a soap, free of undesirable additives. More specifically, the final reac-tion mixture consi~ts of three layers, a fatty ncid top layer, a lipase mixture middle layer and an aqueous glycerin bottom layer,andtheprocessincludesseparatingthetoplayeroffattyacidfromthefi~
reaction mixture, and neutralizing said fatty acids with an alkaline material selected from the group consisting of sodium hydroxide, sodium carbonate and sodium bicarbonate to form a sodium soap substantially free of contaminants. The resulting soap and glycerine is much lighter in color than the corresponding colors after sulfuric acid hydrolysis of fats or the high temperature sodium hydroxide saponification of fats.
More specifically, present invention relates to a process of hydrolyzing a high melting fat into a high yield of fatty acids for use in the production of soap free of under-sirable additives, which comprises reacting a mixture of about 90-75% of a high melting beef tallow and about 10-25% coconut oil, with castor bean lipase, in about 20-50% water acidified to ~ r~c a~
a pH of about 4-5.5 with a weak acid such as acetic acid, ~
phosphoric acid, phosphorous acid and carbonic acid, and at a temperature of about 37C, agitating the reaction mixture for about 3-48 hours, to obtain a final reaction mixture con-sisting of three layers, a fatty acid top layer, a lipase mixture in the middle layer and an aqueous glycerin bottom layer, separ-ating the layers and neutralizing said fatty acids to form soap.
The middle layer which contains active castor bean lipase is reused in~,the hydrolyzation process.
-The llpase enzyme used as a catalyst in the present hydrolysis process may be any animal or vegetable llpase which is non-stereospecific, i.e. the lipase must split the beta (middle carboxyl linkage) glycerlde linkage at about the same rate as splittlng the alpha (outer carboxyl) llnkages.
Suitable examples of non-stereospecific lipase enzymes are derived from castor bean, Candida cylindracea, Propionibacterium acnes, Rhizopus arrhizus, Staphylococcus aureus, Aspergillus flavus and Geotrichum candidum. Most lipases are stereospecific and therefore are ineffective, e.g., porcine pancreatic lipase. Normally the extent of hydrolysis with these enzymes does not exceed 70%, whereas the non-stereospecific enzymes affect substantially complete hydrolysis.
The preferred lipase enzyme utillzed herein is the castor bean lipase (ricinus communis). It is lnsoluble in water, and its activity is materially reduced by contact with water. The enzyme is stabilized by the presence of fats. It is rapidly inactivated by alkalls and functions only in a neutral or slightly acidic medium. This enzyme is activated by the presence of acids, preferably weak acids, such as acetic acid, phosphoric acid, phosphorous acld, carbonlc acid, etc., which exerts the greatest accelerating effect. Accordingly, the optimum temperature for ricinus lipase action is about 37C, and is inactivated at temperatures above about 50C. The amount of lipase used in the hydrolysis process is about 3-15%
of the substrate (fat and oil) by weight, if the lipase activity (LA) is unknown. If the LA is known, approximately one LA unit is used for every 10 microequivalents of potential acid. (See N. Pelch and M. C. Kranz, Anal Biochem, 112, 219-222 ~1981) for a procedure for determination of llpase activity).
~3338~3 An additional advantage in the use of the castor bean lipase in the hydrolysi~ reaction is the ability to recover (~eparate) said lipase from the final reaction mixture and recycle it for use with a fresh substrate (fat and oil). This capability results from the natural immobilization property of ca~.tor bean 1 ipas e .
The castor bean lipase cannot be obtained commercially, but can be prepared as disclosed in the prior art previously discussed. The castor bean lipase utilized herein is prepared by dehulling castor beans, extracting the ~ oils by grinding the dehulled beans in the presence of lowboiling petroleum ether, f ilt ering the ground bean pcnace and discarding the filtrate, i.e.
ether layer containing endogenous oils, repeating the eXtraction and filtration ~teps two more times, air drying the filtered pomace and recovering a lipase preparation in the form of the pomace. Another method of preparing the castor bean lipase enzyme without allergen may also be used. This method of pre-paration should be considered because of the pre~enc e of a potent allergen in the bean. The dehulled bean is macerated in water, rather than petroleum ether and then centrifuged.
The fat layer is separated from the aqueous layer, and the aqueous layer is discarded. Since the lipase is in the spherosome~ along with the endogenous oil, it will remain in the fat layer. The fat layer is extracted with petroleum ether and saturated NAC1 solution. The petroleum ether contain~ the endogenous oil, and is discarded. The saturated NaCl solution contains the lipase in particulate matter.
~ 333893 The optimum condition~ for activity of castor bean lipase i5 about 25-50C and preferably about 37 and a pH of 4 to 5.5. A dilute acid such as O.lN acetlc acid, or other weak acids may be added to bring the pH to approximately 5Ø
The amount of the castor bean lipase enzyme used in the present hydrolysis process should be sufficient to effect a substantial degree of conversion of the beef tallow/coconut oil mixture into fatty acids and glycerol. Amounts of about 3 to 15% and preferably about 10% by weight of thetriglyceride substrate mixture is used.
It has unexpectedly been found that the addition of a vegetable oil such as coconut, corn, soybean, lin~eed, olive and palm oil, to the high melting point beef tallow enables the beef tallow to emulsify at a lower temperature, about 37C. The lipase enzyme hydrolyzes the emulsified tallow/vegetsble oil mixture almost quantitatively at the lower temperature with a resultant savings in energy. The vegetable oil such as coconut oil also hydrolyze~ into fatty acids and glycerol. ThuA, no undesirable extraneous ma~erial~ are present in the reaction mixture, j thereby yielding a substantially pure soap upon neutralization of the fatty acids with sodium hydroxide, sodium carbonate or sodium bicarbonate. The coconut oil constitutes a lesser amount by weight than the beef tallow in a mixture thereof.
The weight ratio of beef tallow to vegetable oil is about 75-90% to about 25-10% vegetable oil.
133389 ~
The data in Table I show that a mixture of high grade edible beef tallow and coconut oil, at the same ratio as may be used in soaps, was hydrolyzed approximately 98~ into fatty acids at the described conditions. This is in clear contrast to results reported in the literature (~eyet ~, ~), ~ ~ch ~fta~ ~ reacted ~th ~tor ~3~ ~e ~t ~ o~yhy~olyz~ d~OUt 3% (Table II). In the latter case, coconut oil was absent.
The combined results clearly show that the addition of coconut oil unexpectedly yields a high conversion of the high melting beef tallow into fatty acids. It is believed that the coconut oil lowered the melting point of the beef tallow enough so that it would form an emulsion at 37C-Table I
:;
Extent of Castor Bean Lipase Hydrolysis of Fats and Oils Observed I Lit, S.V.* Enzyme Hydrolysis Value ; Olive oil (control 186-196 185 83:17 Tallow:coco 203-213 203 j *Saponification Value is the weight (mg) of KOH required to I saponify lg of fat, and is indicative of the extent of hydrolysis of the fats and oils, Table II
Hydrolysis of Beef Tallow by Castor Bean Lipase Duration of Experiment (hrs.) Extent of Hydrolysis (%) 24 2.8 48 2.8 72 2.8 The saponification value is determined by conventional methods described in the literature, M. Applewhite, Kirk-Othmr. Encycl. Chem. Tech., 3rd Ed., 9, 795 (1980). The saponification procedure utilized herein comprises adding 30 ml of absolute ethanol to a 2 to 3g sample in a covered flask, warming on a ~team bath (50-60C), adding 50 ml standardized 0.5N alcoholic KOH solution and boiling for one hour, adding phenolphthalein indicator solution a~d titrating with standardized 0.5N HCl to the disappearance of the pink color, and the ~aponification value is determined.
Saponification of fats and oils in the absence of lipase, to determine the maximum yield of soap availab~e from fats and oils, wa~ conducted on four substrates. Triolein and olive oil are standards used in literature. Beef tallow and coconut oil are components of the soaps prepared herein. The data in Table III shows that the observed results are in fairly good agreement with the literature values for the two standards and coconut oil, but low for beef tallow.
Table III
Saponification Values (SV) Lit Obs.
Triolein 189 (calc) 190 Olive oil 186-196 182 Beef tallow 193-202 173 Coconut oil 250-264 253 .
13338~3 :1, i' The advantages of the enzymatic hydrolysis process of present invention are multifaceted. Higher yields of fatty acids and glycerol are obtained, about 98% conversion. The use of lower temperatures resulted in considerable cost savings.
The use of vegetable oil such as coconut oil which also hydro-lyzes into fatty acids a~d glycerol yields fatty acids and glycerol free of undesirable additives. Ssid fatty acids yield a pure soap upon nuetralization with sodium hydroxide, carbonate, ' or bicarbonate. This low temperature hydrolysis reaction requires less utilization of energy, and therefore, less atmos-pheric pollution i~ produced. The use of a low reaction tem-perature in this process yields fewer undesirable thermal pro-ducts, and a lighter color.
Detailed Descriptioniofithe Invention The following examples are merely illustrative of the invention and are not to be construed as limiting thereof.
Example 1 Preparation of Enzyme Dehull 60g of castor beans. Grind the dehulled beans in a Waring blender in the presence of 600ml of low boiling j (30-60C) petroleum ether. The ether extracts endogenous oils from the beans. Filter and discard filtrate. Repeat extraction and filtration two more timesi. Air dry the crude lipase prepara-tion.
Enzymatic Hydrolysis of Tallow/Coconut Mixture Mix 1.67g of the lipase preparation snd 16.7g of an 83:17 mixture of high grade beef tallow and coconut oil, and 10 cc of O.lN acetic acid. Stir approximately 24 hoursi at 37C. The final mixture consists of 3 layers, fatty acid on the top, lipase mixture in the middle, and sweet water (aqueous glycerine) on the bottom. The fatty acid layer is skimmed off, neutralized with NaOH, Na2CO3, or NaHCO3, and the resulting soap purified in the usual manner of soap manufacturers skilled in the art. The bottom layer is separated, and th~ glycerine removed.
The middle layer, containing more than 50% of the original lipa~e still being active, ls reused after adding a lesser quantity of fresh lipaise.
Analysis of the extent of hydrolysis is determined by adding 3.4 g of reaction mixture to 100 ml absolute alcohol, and tritrating to pH 9.5 with O.lN alcoholic potassiium hydroxide.
Results: 98% conversion to fatty acids and glycerol.
~3~3~93 Example 2 ~reparation of Castor Bean Lipase 60g of dehulled castor beans is macerated in water and centrifuged. The fat layer is separated from the aqueous layer, which is discarded. The fat layer is extracted with 600 ml low boiling petroleum ether (30-60C) and saturated NaCl ; solution. The petroleum ether is discarded, and the NaCl r solution contains the lipase in particulate form. 1.67 g of this particulate lipase is used in the hydrolysis of the tallow/
coconut mixture in accordance with the proces~ of Example 1.
Substan~ially complete hydrolysis into fatty acids and glycerol is obtained.
Example 3 The high melting beef tallow is melted at a temperature of about 42C until it is liquified. The liquified tallow is mixed with the coconut oil in the weight ratio of 80:20 tallow:oil.
1.67g of the lipase preparation of Example 1 is mixed with 16.7 g of the liquified fat and oil mixture, and 10 cc of O.lN acetic acid for about 24 hours at 37C. Substantially complete hydroly~is into fatty acids and glycerol is obtained.
.
i i ~ 3~389~-Other weak acids may be substituted for the acetic acid in tne examples, such as phosphoric, phosphorous or carbonic acid.
Lower melting point fats such as sheep tallow, industrial quality beef tallow, lard and butter may be used in the present lipase hydrolysis process.
Also, other vegetable oils such as corn, soybean, linseed, olive and palm oil may be substituted for the coconut oil in the example It is understood that the foregoing detailed description is given merely by way of illustration and that variations may be made therein without departing from the spirit of the invention. The "Abstract" given above is merely for the convenience of technical searchers and is not to be given any weight with respect to the scope of the inventlon.
Claims (18)
1. A process of converting high melting fats into a high yield of fatty acids and glycerol which comprises hydrolyzing an emulsified mixture of a high melting beef tallow and a vegetable oil in the weight ratio of about 75-90% tallow to 25-10% oil, in an aqueous medium, with an animal or vegetable lipase which is non-stereospecific at a temperature of about 25-50°C and at a pH of about 4-5.5, and recovering a final reaction mixture consisting of fatty acids, glycerol and lipase.
2. The process according to Claim 1, wherein the mixture is agitated for a sufficient period of time of about 3-48 hours to obtain substantially complete hydrolysis into fatty acids and glycerol.
3. The process according to Claim 2, wherein the vegetable oil is coconut oil.
4. The process according to Claim 3, wherein the mixture of the beef tallow and coconut oil is in the weight ratio of 83:17.
5. The process according to Claim 2, wherein the lipase constitutes about 3-15% of the weight of the tallow and vegetable oil mixture.
6. The process according to Claim 2, wherein the lipase enzyme is derived from castor bean, Candida cylindracea, Propionibac-terium acnes, Rhizopus arrhizus, Staphylococcus aureus, Asperigillus flavus and Geotrichum candidum.
7. A process according to Claim 6, wherein the lipase enzyme is a castor bean lipase which is prepared by de-hulling the castor beans, extracting the lipase by grinding the dehulled beans in the presence of a low boiling petroleum ether, filtering the ground beans, extracting and filtering the lipase again, air drying the filtered lipase and recovering a dried castor bean lipase.
8. A process according to Claim 6, wherein the enzyme is a castor bean lipase which is prepared by macerating the dehulled beans in water, centrifuging the mixture to form a fat layer and an aqueous layer, separating the fat layer from the aqueous layer, extracting the fat layer with petroleum ether and saturated sodium chloride solution, and recovering the lipase from the sodium chloride solution.
9. A process of producing high grade soap which comprises hydrolyzing an emulsified mixture of a high melting beef tallow and about 10-25% of a vegetable oil, in an aqueous medium, in the presence of non-stereospecific lipase enzyme, at a temperature of about 25-50°C and at a pH of about 4-5.5, agitating the mixture for a period of time to obtain substantially complete hydrolysis into fatty acids and glycerol, separating the glycerol from the fatty acids, and neutralizing said fatty acid with an alkaline material to form a soap free of undesirable additives.
10. A process according to Claim 9, wherein the alkaline neutralizing material is selected from the group con-sisting of sodium hydroxide, sodium carbonate and sodium bicarbonate.
11. A process according to Claim 9, wherein the lipase enzyme is castor bean lipase.
12. A process according to Claims 7, 8 or 11 wherein the castor bean lipase is separated from the fatty acids and glycerol and is recycled for use in hydrolyzing a fresh mixture of fat and oil.
13. A process according to Claim 9, wherein the vegetable oil is coconut oil.
14. A process of hydrolyzing a high melting fat into a high yield of fatty acids for use in the production of a soap free of undesirable additives, which comprises reacting a mixture of about 90-75% by weight of a high melting beef tallow and about 10-25% coconut oil with castor bean lipase in about 20-50% water acidified to a pH of about 4-5.5 and at a temperature of about 37°C, agitating the reaction mixture for about 3-48 hours to obtain a final reaction mixture consisting of three layers, a fatty acid top layer, a lipase mixture middle layer and an aqueous glycerin bottom layer, separating the top layer from the other layer and neutralizing said fatty acids with a sodium salt selected from the group consisting of sodium hydroxide, sodium carbonate, and sodium bi-carbonate to form soap.
15. A process according to Claim 14, wherein the middle layer containing active castor bean lipase is reused in the hydrolyzation process.
16. A process according to Claim 14, wherein the water is acidified with a weak acid.
17. A process according to Claim 16, wherein the weak acid is citric acid.
18. A process according to Claim 14, wherein the fatty acids are neutralized with sodium hydroxide.
Applications Claiming Priority (2)
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US90842786A | 1986-09-17 | 1986-09-17 | |
US908,427 | 1986-09-17 |
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AR (1) | AR242628A1 (en) |
AU (1) | AU604494B2 (en) |
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CA (1) | CA1333893C (en) |
FR (1) | FR2603900B1 (en) |
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JPH02234684A (en) * | 1989-03-08 | 1990-09-17 | Morinaga Milk Ind Co Ltd | Production of fatty acid calcium salt |
JPH05302100A (en) * | 1992-04-28 | 1993-11-16 | Nisshin Oil Mills Ltd:The | Treatment of edible oil waste |
EP0713917A1 (en) * | 1994-11-28 | 1996-05-29 | Societe Des Produits Nestle S.A. | Process for hydrolysis of polyunsaturated fatty acid triglycerides |
EP0714983A1 (en) * | 1994-11-28 | 1996-06-05 | Societe Des Produits Nestle S.A. | Process for hydrolysis of polyunsaturated fatty acid triglycerides |
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FR328101A (en) * | 1902-10-09 | 1903-12-30 | Llschaft | Process for extracting fatty acids from their ethers |
FR335902A (en) * | 1903-10-14 | 1904-02-20 | Maurice Nicloux | Diastatic saponification of oils and fats, not providing appreciable impurities in the saponification medium |
FR1005898A (en) * | 1947-10-14 | 1952-04-16 | Process for preparing lipase products | |
DK402583D0 (en) * | 1983-09-05 | 1983-09-05 | Novo Industri As | PROCEDURE FOR THE MANUFACTURING OF AN IMMOBILIZED LIPASE PREPARATION AND APPLICATION |
DE3403021A1 (en) * | 1984-01-28 | 1985-08-01 | Henkel KGaA, 4000 Düsseldorf | METHOD FOR PRODUCING MIXTURES FROM C (DOWN ARROW) 6 (DOWN ARROW) -C (DOWN ARROW) 1 (DOWN ARROW) (DOWN ARROW) 0 (DOWN ARROW) FATTY ACIDS |
JPS6135783A (en) * | 1984-07-28 | 1986-02-20 | Agency Of Ind Science & Technol | Preparation of solid fat decomposition enzyme |
FR2585365B1 (en) * | 1985-04-26 | 1987-11-20 | Elf Aquitaine | PROCESS AND APPARATUS FOR ENZYMATIC HYDROLYSIS OF FAT BODIES |
US4629742A (en) * | 1986-01-27 | 1986-12-16 | Akzo America Inc. | Hydrolysis of fats |
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1987
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TR24236A (en) | 1991-07-03 |
FR2603900A1 (en) | 1988-03-18 |
PH26178A (en) | 1992-03-18 |
AU7760387A (en) | 1988-03-24 |
ZW17586A1 (en) | 1987-12-30 |
GB2196337A (en) | 1988-04-27 |
BR8704783A (en) | 1988-05-17 |
IN171359B (en) | 1992-09-19 |
NZ221583A (en) | 1990-10-26 |
AU604494B2 (en) | 1990-12-20 |
ZA876375B (en) | 1989-04-26 |
IT8748392A0 (en) | 1987-09-15 |
FR2603900B1 (en) | 1990-08-24 |
IT1211781B (en) | 1989-11-03 |
MX168282B (en) | 1993-05-14 |
GB2196337B (en) | 1990-07-11 |
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