CA1293083C - Packing material and process for its production - Google Patents

Abstract

ABSTRACT
A packing material for chromatography comprising a carrier having silanol groups on its surface, wherein Si in the silanol groups forms a Si-O-Si bond together with Si in a silyl group of the following formula I and Si in a silyl group of the following formula II, and the molar ratio of the silyl group of the formula II is from 0 to 2/3 based on the total amount of silyl groups:

(I) wherein each of R1 and R2 is a hydroxyl group, a methyl group, an ethyl group, an alkoxy group having from 1 to 3 carbon atoms, a phenoxy group, an acetoxy group, a trimethylsiloxy group, a triethylsiloxy group or an oxygen atom bonded to Si in a silanol group on the surface of the carrier, R3 is a 3-carbonyloxypropyl group, a 1-phenyleneethyl group or a 2-phenyleneethyl group, R4 is a hydrogen atom or a methyl group, and each of m and n is an integer of at least 1; and

Description

t~
Our Ref.: TS-232 PACKING MATERIAL AND PROCESS FOR ITS PRODUCTION
The present invention relates to a packing material for chromatography. More particularly, it relates to a packing material useful particularly for normal phase partition liquid chromatography and aqueous gel permeation chromatography and a process for its production.
As such a packing material for chromatography, there are two types of packing materials i.e. a packing material of inorganic support such as silica gel or porous glass having e.g. 3-glyceryloxypropyl groups, aminopropyl groups or cyanopropyl groups bonded thereto and an organic polymer packing material such as polysaccharide gel, polyacrylamide gel or ion exchange resin. Both types of packing materials are porous and having a hydrophilic large surface. These packing materials are known to be useful as packing materials for normal phase partition chromatography for the separation of polar substances such as saccharides or alcohols, or as packing materials for a~ueous gel permeation chromatography or for hydrodynamic chromatography for the separation of e.g. saccharides, ~' 1~ 3 . proteins, water-soluble sysnthetic polymers based on the molecular sizes.
However, these packing materials are not fully satisfactory, particularly in the following respects:
Namely, the packing material of inorganic support (such as silica gel or porous glass), particularly the one having a hydrophilic stationary phase bonded thereto, is inferior in the durability in a water-containing solvent system and tends to lead to a change with time of the eluting amount even during the use under the same ~ condition. This is believed to be attributable to the ,I slight solubility of the inorganic base material such as silica gel i~ water.
On the other hand, the polymer packing material tends ~ 15 to undergo swelling or contraction depending upon the salt I concentration in the solvent, the pH or the composi-tion of organic solvents. Besides, it is inferior in the mechanical strength as compared with an inorganic porous carrier and is hardly useful in the form of fine 20 particles. This is particularly disadvantageous as a packing material for high performance liquid chromatography wherein the use of fine particles is an ~; importance factor for the improvement of the resolution.
For a packing material for normal phase partltion 25 chromatography or gel permeation chromatography in a water-containing solvent system, it is important that in the water-containing solvent system, it is stable and does not lead to a substantial change in the eluting amount, it does not undergo swelling or contraction due to a change of the mobile phase, and it is hard enough to be useful in the form of fine particles and has a hydrophilic stationary phase.
Now, it has been found that by using a hard inorganic carrier as the base material, a silane having vinyl-polymerizable organic groups is chamically bonded to the surface of the carrier, and then acrylamide is copolymerized to the silane-bonded carrier, whereby not only the silane is simply fixed to the inorganic carrier - by the chemical bond but also the organic groups of the silane are taken into polymer molecules in the form of a copolymer with an acrylamide polymer, and it is possible to obtain a packing material wherein polymer molecules constituting an organic stationery phase are chemical bonded to the carrier by numerous siloxane bonds. It has been found also that with this packing material, no change with time is observed in normal phase chromatography even in the water-containing solvent system, separation based on the molecular sizes can be conducted by aqueous gel permeation chromatography, and it is possible to obtain chromatogram having no substantial change with time.
Thus, it has been possible to thereby solve the above-mentioned problems, and the present invention has been accomplished on the basis of these discoveries.
The present invention provides a packing material for 1~ 3 chromatography comprising a carrier having silanol groups on its surface, wherein Si in the silanol groups forms a Si-O-Si bond together with Si in a silyl group of the following formula I and Si in a silyl group of the following formula II, and the molar ratio of the silyl group of the formula II is from 0 to 2/3 based on the total amount of silyl groups:
--R
H ~ ¢ H - C H2 ~ C Hz - ~ ~ H
¢ 0 NH2 l l (I) . R~- S i - Rz wherein each of Rl and R2 is a hydroxyl group, a methyl - -group, an ethyl group, an alkoxy group having from 1 to 3 carbon atoms, a phenoxy group, an acetoxy group, a trimethylsiloxy.group, a triethylsiloxy group or an oxygen atom bonded to Si in a silanol group on the surface of the carrier, R3 is a 3-carbonyloxypropyl group, a l-phenyleneethyl group or a 2-phenyleneethyl group, R4 is a hydrogen atom or a methyl group, and each of m and n is an integer of at least l; and Rlg R~ R2 (II) wherein each of Rl and R2 is a hydroxyl group, a methyl group, an ethyl group, an alkoxy group having from 1 to 3 ?3~3 ~3 !!

1 carbon atoms, a phenoxy group, an acetoxy group, a l trimethylsiloxy group, a triethylsiloxy group or an oxygen ~' atom bonded to Si in a silanol group on the surEace of the carrier, R5 is a methyl group, an ethyl group or a ' 5 3-glyceryloxypropyl group.-~, The present invention provides also a process for i producing such a packing material for chromatography, :i which comprises reacting silanol groups on the surface of ' the carrier with an organosilane of the following formula III and an organosilane of the following formula IV in such proportions that the molar ratio of the organosilane ~` of the formula IV is from 0 to 2/3 based on the total I amount of organosilanes, at a temperature of from 20 to j 200C to form Si-O-Si bonds, followed by copolymerization 15 with acrylamide at a temperature of from 20 to 200C:
b H2--a--Rï o ,1 . Rg R~ - S i - R7 J I . (III) ,1 20 Rg wherein each of R6 and R7 is a methyl group, an ethyl group, an alkoxy group having from 1 to 3 carbon atoms, a ~ phenoxy group, an acetoxy group, a trimethylsiloxy group, r~ a triethylsiloxy group or a halogen atom, and R8 is an alkoxy group having from 1 to 3 carbon atoms, a phenoxy ~, group, an acetoxy group or a halogen atom, Rg is a 3-carbonyloxypropyl group, a l-phenyleneethyl group, or a ,.

; 83 i 2-phenyleneethyl group, and Rlo is a hydrogen atom or a methyl group; and Rll ~- S i - R7 3 5 R8 (IV) .. . .. .
wherein each of R6 and R7 is a methyl group, an ethyl group, an alkoxy group having from 1 to 3 carbon atoms, a phenoxy group, an acetoxy ~roup, a trimethylsiloxy group, a triethylsiloxy group or a halogen atom, R8 is an alkoxy group having from 1 to 3 carbon atoms, a phenoxy group, an acetoxy group or a halogen atom, and Rll is a methyl ~ group, an e-thyl group or a 3-glycidoxypropyl group.
Now, the present invention will be described in detail with reference to the preferred embodiments.
In the accompanying drawing, Figure 1 is a difference infrared spectrum chart of the packing material of the ~ present invention obtained in Example 6 and the silica gel i -carrier as the starting material.
As the carrier to be used in the present invention, silica gel, porous glass, diatomaseous earth or high silica zeolite may be mentioned. In order to densely cover its surface with the organosilane and further to form a polymer layer wherein acrylamide is bonded to the 1 25 organosilane, the carrier preferably has at least one ! silanol group/nm2, more preferably at least two silanol groups/nm2, on its surface. The carrier may have any 1~t3~3 ~ optional shape. However, a spherical shape is preferred ; particularly for liquid chromatography. The carrier i preferably has a particle size of from 1 to 500 ~m, more ; preferably from 1.5 to 200 ~m. In the case of a carrier having pores, it preferably has an average for size of at ' least 30 A.
The copolymer covering the surface of the carrier of the present invention is represented by the formula I, which is bonded to Si in the silanol groups on the surface of the carrier by a Si-O-Si bond. The copolymer is the one formed by the polymerization of the vinyl group portion of the vinyl group-containing organosilane of the formula III with the vinyl group portion of acrylamide, whereby an excellent hydrophilic carrier durable against a water-containing eluting solution system can be formed.
Further, the cover for the carrier may not necessary be composed solely of said copolymer, and so long as said copolymer can adequately cover the surface of the carrier, a silyl group of the formula II may be bonded by a Si-O-Si bond to a silanol group on the surface of the carrier.
The molar ratio of the silyl group of the formula II must be within a range of from 0 to 2/3 based on the total silyl groups. If the molar ratio exceeds 2/3, the cover by said copolymer will be inadequate, such being undesirable.
With respect to the 3-carbonyloxypropyl group, the l-phenyleneethyl group or the 2-phenyleneethyl group for .3~33 R3 in the formula I or for Rg in the formula ~ the propyl group or the ethyl group is bonded to Si in the organosilane, and the carbonyl or the phenylene is bonded to the carbon atom which is bonded to R4 or Rlo.
! 5 The packing material of the present invention can be obtained by reacting silanol groups on the surface of the carrier and the organosilane to form Si-O-Si bonds, followed by copolymerization with acrylamide.
'. As the vinyl group-containing organosilane, it is common to employ an organosilane of the formula III. The . organosilane of the formula III includes, for example, styrylethyltrimethoxysilane, methacryloxypropyldimethylchlorosilane, 3-methacryloxypropylmethyldiethoxysilane, 3-methacryloxypropyltrimethoxysilane, 3-methacryloxypropyltris(methoxyethoxy)silane, 3-acryloxypropyltrimethoxysilane, 3-acryloxypropylmethyldimethoxysilane.
As another organosilane having no vinyl group, a functional group having strong hydrophobic nature is undesirable, and it is common to employ an organosilane of the formula IV. The organosilane of the formula IV
includes, for example, 3-glycidoxypropyldimethyl-ethoxysilane, 3-glycidoxypropylmethyldiethoxysilane, 3-glycidoxypropyltrimethoxysilane, methylbromodichloro-silane, methyldimethoxychlorosilane, methyltriethoxysilane, methyltriacetoxysilane, 1~ 3(~3 J -- 9 _ dimethyldiethoxysilane, triethylchlorosilane, . methoxyethylmethyldichlorosilane, trimethylchlorosilane, trimethyl-n-propoxysilane, and trimethyldisilane.
;, In the present invention, for the Eormation of Si-O-Si ' 5 bonds by the reaction of silanol groups on the surface of ! the carrier with such organosilanes, a conventional method may be employed. The proportions of the organosilane having a vinyl group and the organosilane having no vinyl ~ group are such that the organosilane having no vinyl group ¦ 10 must be present in a molar ratio of not higher than 2/3 relative to the total amount of silanes. Within the range I of such proportions, the vinyl group-containing ~! organosilane alone, or the vinyl group-containing ~ organosilane and the organosilane having no vinyl group f 15 together or separately, may be reacted to the silanol , groups of the carrier. It is preferred to employ a ¦ solvent from the viewpoint of the reproducibility of the reaction or operation efficiency. However, the reaction may be conducted in the absence of a solvent.
The solvent may be any solvent so long as it is capable of dissolving the organosilanes and inert to the carrier and to the organosilanes. For examples, ethers such as n-butyl ether, ethylene glycol dimethyl ether, diethylene glycol dimethyl ether, tetrahydrofuran and ~ 25 dioxane, hydrocarbons such as toluene, xylene and '! ' n-hexane, halogenated hydrocarbons such as chlorobenzene and dibromobutane, amid^s such as dimethylformamide, 1~3~i ~3 diethylacetamide, and L~-methylpyrrolidone, and sulfoxides such as dimethylsulfoxide, may be used alone or in combination as a mixture.
When the reaction temperature is low, the reaction rate is slow, and the amount of the organosilanes bonded will be inadequate. On the contrary, if the reaction temperature is high, polymerization of the unsaturated organic groups by heat is likely to take place.
Therefore, the reaction is conducted usually within a temperature range of from 20 to 200C, preferably from 40 to 150C.
In order to prevent the progress of the polymerization reaction during the bonding of the organosilanes, a polymerization inhibitor may be added in an amount of not higher than 1% relative to the amount of the organosilanes. As such a polymerization inhibitor, a phenol or a hydroquinone commonly employed, may be used.
The amount of the organosilane relative to the porous carrier depends on the amount of silanol groups present in the carrier. The organosilane having an unsaturated organic group to be used in the present invention is capable of bonding only in an amount of 4 ~mol/m2 at best, and therefore, the organosilane may be used in an amount of at least (surface area m2 of the carrier) x (4 ~mol/m2).
Then, the silane-treated carrier obtained by the above reaction is copolymerized with aFrylamide. As a method ~ 11 --for this copolymerization, there may be employed a method wherein acrylamide and a polymerization initiator are supported on the surface of the silane-treated carrrier, followed by suspension polymerization in a solvent in which acrylamide is insoluble or by polymerization under heating in the absence of a solvent, or a method wherein the carrier, acrylamide and a polymerization ini-tiator are mixed in a solvent in which acrylamide i5 soluble, followed by polymerization. There is no particular restriction to the initiator to be used here. An initiator commonly used for the polymerization of acrylamide such as a peroxide, an azo compound or a redox initiator may be employed.
, .
The solvent capable of dissolving acrylamide and 15 useful for the polymerization includes water and water-soluble organic solvents such as methanol, ethanol, isopropyl alcohol, acetonitrile, ethylene glycol, ~ diethylene glycol, glycerol, methylcarbitol, r~, dimethylformamide, dimethylacetamide, dimethylsulfoxide, ~ 20 dioxane and acetone, and a mixtures thereof.
s The solvent in which acrylamide is insoluble and which is useful for suspension polymerization of the acrylamide-supported silane-treated carrier, includes hydrophobic solvents such as n-hexane, kerosine, , 25 n-paraffin, benzene, toluene, xylene, methyl isobutyl ketone, l,l-trichloroethane, carbon tetrachloride, dibutyl ether and chlorobenzene, and solvent mixtures there~f.

.~ .
;7 ;

3~ ~3 t - 12 -In the above copolymerization reaction, the ~, polymerization may proceed at low temperatures, but the reaction rate will usually be too slow to be practical.
, On the other hand, at high temperatures, various side ! 5 reactions are likely to take place, and the life of generated radicals tends to be short, whereby crosslinking among organosilanes tends to be inadequate. Accordingly, the life of the packing material in an aqueous solvent will be short, or irreversible adsorption will be caused, - 10 such being undesirable. Specifically, the copolymerization is conducted usually within a temperature - range of from 20 to 200C. The temperature is selected ~S' primarily depending on the type of the initiator. The copolymerization is preferably conducted within a range of from 30 to 150C.
I The packing material thus ob-tained contains ¦ polyacrylamide not chemically bonded to the packing material and unreacted monomer. Such polymer and monomer are soluble in water and can readily be removed by 20 washing.
The packing material of the present invention has excellent properties as a packing material for aqueous gel permeation chromatography or for normal phase partition chromatography, as compared with the conventional 25 carriers. The features as the packing material for aqueous gel permeation chromatography include the low adsorbing properties and the capability of providing 3~83 highly reproducible data. Namely, the interaction between the carrier surface and the solute is minimum, since the porous surface of the carrier is covered with a layer of polyacrylamide which is a hydrophilic polymer. Further, since the polymer layer is bonded to the silica gel by numerous siloxane bonds, the organic stationary phase will be scarcely released from the carrier even by the hydrolysis of siloxane bonds or by the equilibrium reaction of dehydration and condensation which take place in the water-containing solution. Thus, highly reproducible results of measurement can be obtained even when the packing material is used in a water-containing solution for a long period of time. Yet, the base material for the carrier may be silica gel or porous glass which is an inorganic carrier prepared by a conventional method. Such a carrier is a hard porous material which usually has a pore size distribution narrower than that of an organic polymer carrier and which undergoes no substantial change in the swelling degree due to a change of the solvent. Thus, it can be used in the form of fine particles, whereby high resolution (or performance) can be obtained and high speed separation will readily be possible. The pore size depends on the carrier as the base material. The base materials having various pore sizes can be prepared by conventional methods. Thus, the hydrophilic carrier obtained by the present invention is extremely effective as a packing material for aqueous gel 1 ~3~

permeation chromatography for the separation based on the molecular sieve effect in an aqueous solution system of e.g. proteins, polypeptides, nucleic acids, nucleotides, saccharides, or water-soluble synthetic polymers.
On the other hand, similar features are utilized also as a packing material for normal phase partition chromatography. It is excellent in the resolution and in the reproducibility particularly for the separation of polyols or saccharides by normal phase partition chromatography in a water-containing solvent system. Also in a known-aqueous solvent system, it exhibits a performance substantially equal to that of conventional inorganic carrier base packing materials.
Now, the present invention will be described in further detail with reference to Examples. However, it should be understood that the present invention is by no means restricted to such specific Examples. In the Examples, "parts" means "parts by weight".

Silica gel used as the base material had the following physical properties.
Shape: spherical, particle size: 8-12 ~m, specific surface area: 250 m /g, average pore size: 250 ~.
This silica gel was dried in a reduced pressure drier at 80C under 10 mmHg for 16 hours. To 10 parts of this dried silica gel, 2 parts of 3-acryloxypropyl-l.~"t3~83 trimethoxysilane, 50 parts o~ toluene, 0.01 part of phenol and 4 parts of diethylaminoethanol were mixed, and the mixture was reacted at 75C for 3 hours. The resulting silane-treated silica gel was collected by filtration, then washed twice with 50 parts of toluene and then -three times with 50 parts of methanol and dried under reduced pressure at 30C for 16 hours. 10 parts of the silane-treated silica gel thus obtained was suspend~d in 50 parts of a 25% methanol aqueous solution, and 1 part of acrylamide and 0.1 part of potassium persulfate were added thereto. The reaction system was flushed with nitrogen, then heated to 60C and polymerized for 6 hours. The carrier thus obtained was collected by filtration and repeatedly washed with water to remove polyacrylamide not bonded to the carrier. The packing material thus obtained was packed into a stainless steel column having an inner diameter o~ 7.5 mm and a length of 30 cm by a slurry-packing method, and its properties as a packing material for gel permeation chromatography in an aqueous solvent system were examined under the following conditions:
Apparatus:
Pump: CCPD high pressure pump, detector: RI-8 (differential refractometer), W -8 (ultraviolet absorption meter 280 nm), all manuEactured by TOSOH CORPORATION

~3~2,3 Conditions for measurement:
Eluent: water or 20 mM phosphate buffer solution (pH 7.0, containing 0.1 M sodium chloride), flow rate: 1 ml/min, amount of the loading sample: 0.1~ 50 ~1 (proteins), 0.1-0.5~ 50 ~1 (others) Firstly, a mixture of dextrane (MW: about 500,000, Dextrane T-500, manufactured by Pharmacia AB) and ethylene glycol was measured by using water as the eluent, and the gel capacity was obtained.
(Gel capacity) = (Eluted amount of ethylene glycol) -(Eluted amount of Dextrane T-500)/(Eluted amount of Dextrane T-500) The gel capacity here was 1.20.
Then, the elution volumes and the recovery rates of various proteins were measured by using the phosphate buffer solution as the eluent. The eluent was permitted to flow under the same condition for futher 200 hours, and the elution volumes and the recovery rates of various proteins were again measured. The results are shown in Table 1. The recovery rate is represented by a percentage of the sum of the surface areas of the peaks (main peaks and peaks of impurities) of ultraviolet absorption by proteins when the sample is passed through the packed column and the surface areas of the peaks of ultraviolet absorption by proteins when the sample is passed through a stainless steel column having an inner diameter of 0.6 mm and a length of 3.7 m. The measurement was repeated three times, and the average of the measured values was obtained.
The results of the analysis of the composition in the dried state of the carrier after the silane-treatment and of the carrier after the copolymerization reaction in the present Example are shown below.
C% H% N% SiO2% Others After silane treatment: 5.6 1.2 less than 91.4 1.8 0.3 After copolymeri-zation: 9.3 1.5 2.0 84.0 3.2 To 10 parts o~ the same silica gel as used in Example 1, 3 parts of 3-glycidoxypropyltrimethoxysilane and 50 parts of toluene were mixed, and the mixture was reacted at 100C for 3 hours. The treated silica gel was collected by filtration and washed once with 50 parts of toluene, then twice with 50 parts of acetone and further twice with 50 parts of pure water. The silane-treated silica gel thus obtained was mixed with 100 parts of an aqueous solution of hydrochloric acid (pH: 3.0), and the mixture was reacted at 40C for 3 hours to subject the epoxy group of the 3-glycidyloxypropyl group to hydrolysis for ring-opening. The product was washed with water and then with methanol and dried under reduced pressrue. The ' l.?~ ! 83 carrier thus obtained was packed in a wet system in the same column as used in Example 1, and the eluted amounts of proteins were measured under the same condition as in Example 1. The results are shown in Table 1.

To 10 parts of the same silica gel as used in Example 1, 3 parts of 3-glycidoxypropyltrimethoxysilane, 0.5 part of 3-methacryloxypropyltrimethoxysilane, 50 parts of toluene and 0.01 part of phenol were mixed, followed by silane treatment and copolymerization with acrylamide in the same manner as in Example 1. The carrier thus obtained was subjected to the ring-opening of the g remaining epoxy group in an aqueous hydrochloric acid solution in the same manner as in Example 1, and the carrier thus obtained was packed in a column in the same manner as in Example 1, and the elution volumes of proteins were measured under the same condition as in Example 1. The results are likewise shown in Table 1.

By using the same silica gel as used .in Example 1 and various organosilanes, catalysts and solvents as identified in Table 2, the reaction was conducted under the same condition as in Example 1 to obtain carriers.
. The carbon and nitrogen contents in each carrier are shown in Table 2. Each carrier was packed in the same column as used in Example 1, and the recovery rates of proteins (human serum Y-globulin, egg albumin, cytochrome C) were `; ~ Ç ~3 determined under the same condition as in Example 1. The results are shown in Table 2. However, in Example 3, the réaction temperature for the silane treatment was 50C, which was different from the reaction temperature in Example 1.

To 10 parts of the same silica gel as used in Example 1, 2 parts of styrylethyltrimethoxysilane, 2 parts of 3-glycidoxypropylmethyldiethoxysilane, 50 parts of toluene and 0.01 part o~ phenol were mixed, and the mixture was reacted at 110C for 3 hours. The silane-treated silica gel thus obtained was collected by filtration, washed twice with 50 parts of toluene and then three times with 50 parts of methanol and dried under reduced pressure at 40C. Then, 10 parts of the dried gel thus obtained was mixed with 0.2 part of 2,2~-azobis(isobutylamide)-dihydrate, 2 parts of acrylamide and 40 parts of a 90 methanol aqueous solution, and then the solvent was removed under reduced pressure. The carrier thus obtained was suspended in xylene and reacted for polymerization at 80C for 6 hours. The product was collected by filtration and washed three times with 50 parts of acetone and then thoroughly washed with water. The carrier thus obtained was mixed with 100 parts of an aqueous hydrochloric acid solution (pH 3), and the mixture was reac-ted at 40C for 3 hours to subject the epoxy group in the 3-glycidoxypropyl group to ring-opening. The carrier thus obtained was 1.~ 83 packed in a column in the same manner as in Example 1, and the measurement of proteins were conducted under the same condition as in Example 1. The results are shown in Table 1.
The results of the analysis of the composition in a dried state of the carrier thus obtained are shown below.

C% H% N% SiO2% Others Packing material 10.2 1.5 2.0 82.0 3.3 of Example 5 ~' .

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1~3~ ~3 EXAMPL~ 6 The physical properties o silica gel used as the substrate were as follows:
Shape: spherical, particle size: 6-9 ~m, specific surface area: 330 m2/g, average pore size: 100 A
This silica gel was dried in a reduced pressure drier at 80C under 10 mmHg for 16 hours. To 10 parts of the dried silica gel, 2 parts of 3-methacryloxypropyl-trimethoxysilane, 50 parts of toluene and 0.1 part of phenol were mixed, and the mixture was reacted at 80C for 3 hours. The silane-treated silica gel was collected by filtration, then washed twice with 50 parts of toluene and then three times with 50 parts of methanol and dried under reduced pressure at 30 C for 16 hours. 10 Parts of the silane-treated silica gel thus obtained was suspended in 100 parts of a 2S% methanol a~ueous solu-tion, and 12 parts of acrylamide and 0.2 part of potassium persul~ate were added thereto. The reaction system was flushed with nitrogen, then heated to 60C and reacted for 8 hours.
Water was added to the carrier suspension thus obtained, and the carrier was separated by centrifugal sedimentation and repeatedly washed with water to remove polyacrylamide not fixed to the carrier. The packing material thus obtained was packed in a stainless steel column having an inner diameter of 4.6 mm and a length of 25 cm, and the properties as a packing material for normal phase i3 partition chromatography were examined under -the following condition. The apparatus was the same as used in Example ., 1.

The condition for measurement:
Eluent: mixture of acetonitrile and water (volume ratio: 75/25, flow rate: 1.0 ml/min, amount of the loading sample: 0.2%, 20 ~1, sample: polyols (glycerol, erythritol, xylitol, mannitol, inositol) The eluted amounts of the polyols are 5.6 ml, 6.9 ml, 10 8.5 ml, 10.2 ml and 18.0 ml, respectively. The respective components were completely separated from one another.
After running for 200 hours under the condition for measurement, there was no change in the eluted amount of each component, and no change in the column efficiency was observed. Thus, good data for reproducibility were obtained. The results of the analysis of the composition in a dried state of the carrier after the silane treatment and after the copolymerization reaction are shown below.

C% H% N% SiO2% Others %

After silane treatment: 6.0 1.1 less than 89.7 3.0 0.3 After copoly-25 merization:12.2 2.0 2.4 80.6 2.8 The difference spectrum of infrared absorption analyses of the carrier and the silica gel substrate after the copolymerization was measured, whereby absorption at 1.~ 33 3,200 cm 1 attributable to hydrogen ~ond N-H, at 2,950 cm 1 attributable to aliphatic C-H at 1,675 cm 1 attributable to C=O of an amide, at 1,610 cm 1 attributable to NH2 of an amide and at 1,320 cm 1 attributable to C-N was observed, which indicates that the organic stationary phase comprises polyacrylamide as the main constituting units. The infrared spectrum is shown in Figure 1.

To 10 parts of the same silica gel as used in Example 6, 4 parts of 3-aminopropyltriethoxysilane and 50 parts of toluene were mixed, and the mixture was reacted at 90C
for 3 hours. The treated silica gel was collected by filtration and washed once with 50 parts of toluene and three times with 50 parts of acetone. The packing material thus obtained was packed in the same column as used in Example 6, and the eluted amounts of polyols were measured by using the same appara-tus under the same condition as in Example 6. The eluted amounts of glycerol, erythritol, xylitol, mannitol and inositol immediately after the packing were 5.1 ml, 6.1 ml, 7.4 ml, 8,7 ml and 15.0 ml, repsectively. However, after running for 72 hours under the condition for measurement, eluted amoun-ts of the respective polyols were 4~0 ml, 4.6 ml, 5.2 ml, 5.9 ml and 9.0 ml, respectively, although no substantial change in the column efficiency was observed.

Claims (8)

1. A packing material for chromatography comprising a carrier having silanol groups on its surface, wherein Si in the silanol groups forms a Si-O-Si bond together with Si in a silyl group of the following formula I and Si in a silyl group of the following formula II, and the molar ratio of the silyl group of the formula II is from O to

2/3 based on the total amount of silyl groups:

(I) wherein each of R1 and R2 is a hydroxyl group, a methyl group, an ethyl group, an alkoxy group having from 1 to 3 carbon atoms, a phenoxy group, an acetoxy group, a trimethylsiloxy group, a triethylsiloxy group or an oxygen atom bonded to Si in a silanol group on the surface of the carrier, R3 is a 3-carbonyloxypropyl group, a 1-phenyleneethyl group or a 2-phenyleneethyl group, R4 is a hydrogen atom or a methyl group, and each of m and n is an integer of at least 1; and (II) wherein each of R1 and R2 is a hydroxyl group, a methyl group, an ethyl group, an alkoxy group having from 1 to 3 carbon atoms, a phenoxy group, an acetoxy group, a trimethylsiloxy group, a triethylsiloxy group or an oxygen atom bonded to Si in a silanol group on the surface of the carrier, R5 is a methyl group, an ethyl group or a

3-glyceryloxypropyl group.
2. The packing material according to Claim 1, wherein the carrier has at least one silanol group/nm2 on its surface.
3. The packing material according to Claim 1, wherein the carrier is spherical porous particles having a particle size of from 1 to 500 µm and an average pore size of at least 30 .ANG..

4. A process for producing a packing material for chromatography, which comprises reacting silanol groups on the surface of a carrier with an organosilane of the following formula III and an organosilane of the following formula IV in such proportions that the molar ratio of the organosilane of the formula IV is from 0 to 2/3 based on the total amount of organosilanes, at a temperature of from 20 to 200°C to form Si-O-Si bonds, followed by copolymerization with acrylamide at a temperature of from 20 to 200°C:

(III) wherein each of R6 and R7 is a methyl group, an ethyl group, an alkoxy group having from 1 to 3 carbon atoms, a phenoxy group, an acetoxy group, a trimethylsiloxy group, a triethylsiloxy group or a halogen atom, and R8 is an alkoxy group having from 1 to 3 carbon atoms, a phenoxy group, an acetoxy group or a halogen atom, R9 is a 3-carbonyloxypropyl group, a 1-phenyleneethyl group, or a 2-phenyleneethyl group, and R10 is a hydrogen atom or a methyl group; and (IV) wherein each of R6 and R7 is a methyl group, an ethyl group, an alkoxy group having from 1 to 3 carbon atoms, a phenoxy group, an acetoxy group, a trimethylsiloxy group, a triethylsiloxy group or a halogen atom, R8 is an alkoxy group having from 1 to 3 carbon atoms, a phenoxy group, an acetoxy group or a halogen atom, and R11 is a methyl group, an ethyl group or a 3-glycidoxypropyl group.

5. The process according to Claim 4, wherein the organosilane of the formula III is selected from the group consisting of styrylethyltrimethoxysilane, methacryloxypropyldimethylchlorosilane, 3-methacryloxypropylmethyldiethoxysilane, 3-methacryloxypropyltrimethoxysilane, 3-methacryloxypropyltris(methoxyethoxy)silane, 3-acryloxypropyltrimethoxysilane, 3-acryloxypropylmethyldimethoxysilane.

6. The process according to Claim 4, wherein the organosilane of the formula IV is selected from the group consisting of 3-glycidoxypropyldimethylethoxysilane, 3-glycidoxypropylmethyldiethoxysilane, 3-glycidoxypropyltrimethoxysilane, methylbromodichlorosilane, methyldimethoxychlorosilane, methyltriethoxysilane, methyltriacetoxysilane, dimethyldiethoxysilane, triethylchlorosilane, methoxyethylmethyldichlorosilane, trimethylchlorosilane, trimethyl-n-propoxysilane, and trimethyldisilane.

7. The process according to Claim 4, wherein the organosilane of the formula III is used in an amount of at least 4 µmol per m2 of the surface area of the carrier.

8. The process according to Claim 4, wherein the reaction is conducted a-t a temperature of from 40 to 150°C, and the copolymerization is conducted at a temperature of from 30 to 150°C.