CA1156542A - Culture swab kit and method of utilizing same - Google Patents

Culture swab kit and method of utilizing same

Info

Publication number
CA1156542A
CA1156542A CA000362110A CA362110A CA1156542A CA 1156542 A CA1156542 A CA 1156542A CA 000362110 A CA000362110 A CA 000362110A CA 362110 A CA362110 A CA 362110A CA 1156542 A CA1156542 A CA 1156542A
Authority
CA
Canada
Prior art keywords
compartment
swab
film package
seal
package
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
CA000362110A
Other languages
French (fr)
Inventor
Brian R. Pike
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Application granted granted Critical
Publication of CA1156542A publication Critical patent/CA1156542A/en
Expired legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/02Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00Means for pre-treatment of biological substances
    • C12M45/22Means for packing or storing viable microorganisms

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A B S T R A C T

A culture collecting kit and a method of taking a culture sample are disclosed herein. The culture collecting kit comprises a sterile film package which is divided to provide first and second compartments. A growth media jelly is located in the first compartment and a swab is located in the second compartment. A rupturable seal is provided at a free end remote from the first compartment for providing access to one end of the swab. The swab includes a stick having a head which carries means for taking the culture sample. A first transverse rupturable seal is located between two transverse permanent seal portions which project into the package between the compartments. The transverse permanent seal portions define a tapered guide at the end of the second compartment adjacent the first compartment for guiding the swab head against the rupturable seal. The method of taking the culture sample comprises steps of provid-ing a growth medium jelly in the first compartment, opening the film package by rupturing the pealable seal to expose an end portion of the stick, grasping only the end portion, removing the swab from the second compartment, utilizing the swab to obtain a culture sample, initiating return of the swab into the second compartment, breaking off the stick end portion, removing an end portion of the film package between a new end and the stick and the pealable seal and then reseal-ing the film package.

Description

1 ~5~542 This invention relates in general to new and useful improvements in film packages, and more particularly to a culture swab kit.
At the present time, culture swab kits are rela-tively expensive in view of the package construction. A
preferred culture swab kit includes a first tube having a rounded closed end and an open end and second tube having a rounded closed end and an open end. The second tube is telescoped over the open end of the first tube and is initially seated relative thereto. A swab is carried by the second tube with the second tube being usable as a handle. The first tube has an end portion disposed beyond the swab wherein a transplant medium jelly is stored. The jelly is separated from the swab by means of a piston which loosely fits within the first tube.
When the existing culture swab kit is utilized, the seal between the two tubes is broken and the second tube is utilized as a handle for the swab. After the culture has been obtained, the swab is again placed in the first tube and the second tube is telescoped over the open end of the first tube. The second tube is then pushed down on the first tube so that the swab engages the piston forcing the piston against the jelly with the jelly extruding around the piston and surround the swab.
It will be readily apparent that the provision of two separate tubes and the necessity of securing the swab to one of the tubes is quite an expensive operation. Also, the provision of the piston after the jelly has been disposed in the first tube is also expensive.
In accordance with this invention, it is proposed 1 1565~

to provide a culture swab kit which is Eormed from plastic film having seals which may be readily machine made. Thus, i~n accordance with this invention, it is possible to form a culture swab kit which is relatively inexpensive as com-pared to those which are now available.
Another feature of the invention is the provisionof a culture swab kit wherein the film package may be readily resealed after the swab has been utilized to obtain the desired culture and the film package, when resealed, remains impervious.
In accordance with this invention, it is proposed that the film package be provided with a peelable seal which initially permits access to the culture swab, and the culture swab including a stick having a handle portion which is removable therefrom thereby after the culture has been obtained, the swab may be replaced in the film package and that portion thereof which has been handled may be removed. At the same time, an end portion of the original film package is removed so that in the eventthe end portion of the film package should be subject to contamination, this is removed.
Further in accordance with this invention, the shortened film package may be resealed utilizing a label and once the film package reaches a laboratory for testing, the film package may be again torn with the newly sealed end portion being removable together with the swab for testing.
The reclosed end portion is provided with a label which identifies the swab and this label stays with the swab as the culture carried thereby is being tested for identifica-tion purposes.
With the above, and other objects in view that -if`~` will hereinafter appear, the nature of the invention will be more .3 ~ 2 -11~6542 .

clearly understood by reference to the ~ollowing detailed description, the appended claims and the several views illustrated in the accompanylng drawings, Figure l i9 a perspective view, with parts broken away and shown in section, o~ the culture swab kit formed in accordance with this invention, Figure 2 is a schematic perspective view of the culture swab kit with the peelable seal thereof open:and there being access to an end of the culture swab;
Figure 3 is a schematic perspective view showing the swab being utilized to obtain a culture, Figure 4 is an exploded perspective view with parts broken away and shown in section of the culture swsb kit with the swab returned to the film package and the end thereoi placed in a growth media jelly and with the contaminated end portion of the stick of the culture swab being removed together with an end portion oi the film package, Figure 5 is a perspective view of the reclosed ~0 ~ilm package, Figure 6 is a schematic perspective view showing the reclosed iilm package ruptured along a second rupture line with the culture swab removed therefrom and the removed end portion of the ~ilm package including the identi~ication label being attached to the swab for use as a handle, Re~erring now to the drawings in detaill it will be seen that there is illustrated in Figure 1 the culture swab kit which is t~e subject of this invention, the kit being generally identified by the numeral 10, The kit 10 includes a ~ilm package, generally identified by the numeral 12, .
~ 3 - - :

"~ ~ 115B~4~

The film package is formed of two film sheet portions 14 and 16 which are primarily bonded together by a U-shaped permanent seal 18, which seal may be a heat seal. The remainder of the seal between the films 14 and 16 is a generally chevron shaped seal 20 which is a peelable seal and thus different from the seal 18. The chevron seal 20 leaves two flaps 22, 24 which are readily graspable to peel the chevron seal 20.
The film package 12 also includes a rupturable seal 26. The rupturable seal 26 is preferably in accordance with the teach of U.S. Patent No. 3,608,709 but may be of any type of rupturable seal.
With respect to the rupturable seal 26, it will be seen that it is re~atively short and extends between two triangular portions 28, 30 of the U-shaped seal 18.
The seal portions 28 and 30, in view of their triangular configuration, define a guideway 32 to be desribed in detail hereinafter.
Most specifically, the rupturable or breakable seal 26 divides the film package 10 into a first compartment 34, which is relatively short, and an elongated second compartment 36. It is also to be noted that the compartment 36 may be subdivided by tearing of the film package 12 transversely by the provision of two pairs of notches 38 and 40 formed in the side edges thereof and terminating within the seal 18.
In accordance with this invention, a growth medium jelly 41 is placed within the first compartment 34. The second compartment 36 contains a culture swab, generally identified by the numeral 42. The culture swab 42 includes --` 1156S42 an elongated stick 44 which is provided at one end thereof with a head 46 which is formed of a suitable culture taking material, such as cotton. It is to be noted that the stick 44, which is perferably in the form of a plastic material tube, is provided with a weakening line 48 so as to divide the stick 44 into a main portion 50 and a removable handle portion 52.
It is to be understood that the culture swab kit 10 will be sterilized in any conventional manner so that the interior of the film package 12, the jelly 41 and the swab 42 will all be sterilized.
When the kit 10 is to be utilized, the film package 12 is opened by peeling the peelable seal 20 by grasping the flaps 22 and 24 and pulling them apart. As is best shown in Figure 2, once the film package 12 is initially opened, the handle portion 52 of the swab 42 can be readily grasped and the swab 42 removed from the film package.
Next, the swab, while being grasped by the handle portion 52, is utilized to obtain a culture, as is schema-tically shown in Figure 3. The swab is then returned to thefilm package and the head 46 is guided by the guide 32 against the rupturable seal 26 and then sufficient pressure is applied to the stick 44 so that the head 46 ruptures the seal 26 and the head 46 enters into the jelly 41. At this time the open end portion of the second compartment 36 may be removed by tearing the film package 12 along a line extending between the notches 38. At the same time, the handle portion 52 of the stick 44 may be removed by breaking it along the weakening line 48. This is clearly shown in Figure 4 with the removed end portion of the film package being identified by the ; 1156542 ,.
numeral 54. The film package is now ready ~or resealing, The film package 12 is resealed by folding over the iree end oi the iilm package deiining the second com-partment 36 to deiine a sealing flap 56 as is clearly sbown in Figure ~. The sealing ilàp 56 is then held iD place by the application oi a label 58 which is preferably an identification label. The label 58 serves to completely seal the film package once again. The film package is now ready ~or transportation to a laboratory to test the culture on the head 46 of the culture swab 42.
When the iil~ package 12 is received at the test facility, it is torn once again transversely of the second compartment 36 along the line defined by the notches 40.
As a result, a second end portion of the film package 12 i~ iormed, this second end portion being identiiied by th~
numeral 60. Once the end portion 60 has been torn from the remainder of the film package, it may be utilized as a handle ior gripping the remaining portion 50 of the stick 44 to facilitate the removal of the culture swab 42 from the film package and placing the sæme in a suitable test iacility,~uch as a petrie dish. The film package portion 60 remains with the culture swab 42 so that the identifica-tion label 58 may be utilized to identify the culture sample being tested.
It is to be understood that the material used to ~ake the iilm package 12 must come from the family oi heat sealing thermoplastics in order for the most efficient manuiacturing process to be used. The materisl from which the film package 12 would most likely be made would come irom the categories oi materials including cellophane andt' polyethylene lamination~ and the like, It is also to be understood that the laminations may include metal foils and that the iilms 14 and 16 may be o~ many lAyers, It i~ also to be readily apparent ~rom the fore-going description of'the usage of the culture swab kit that at no time ifi the culture subject to contamination, Altbough only a pre~erred embodiment of the ioventioo has been specifically illustrated and described hereio, it i8 to be understood that minor variations may be made without departing,from the spirit and ~cope o~.
the invention, a~ defined by the appended claims.

. - 7 _

Claims (10)

I claim:
1. A culture collecting kit comprising an axially elongated sterile film package, a transverse first rupturable seal dividing said package into a growth media jelly first compartment and a culture taking swab second compartment, said compartments being in axially adjacent sealed relation, a growth media jelly in said first compartment and a swab in said second compartment, said film package having a second rupturable seal at a free end thereof remote from said first compartment for providing access to one end of said swab, said swab including an elongated stick having a head carrying means thereon for taking a culture sample, said swab head being axially adjacent said first compartment, said transverse first rupturable seal being intermediate two transverse permanent seal portions projecting into said package between said compartments, said transverse permanent seal portions defining a tapered guide at the end of said second com-partment axially adjacent said first compartment for guiding said swab head against said rupturable transverse seal for rupturing said transverse seal and for movement axially from said second compartment into said first compartment.
2. A culture collecting kit comprising an axially elongated sterile film package, a transverse first rupturable seal dividing said package into a growth media jelly first compartment and a culture taking swab second compartment, said compartments being in axially adjacent sealed relation, a growth media jelly in said first com-partment and a swab in said second compartment, said film package having a second rupturable seal at a free end thereof axially remote from said first compartment for providing access to one end of said swab, said swab including an elongated stick having a head carrying means thereon for taking a culture sample, said swab head being axially adjacent said first compartment, transverse weakening means for facilitating removal of an end portion of said second compartment axially spaced from said first compartment less than the length of said swab, said second rupturable seal terminating at least at said transverse weakening means wherein said film package when said end portion is removed is otherwise intact, and said stick having means for facilitating removal of an end portion of said stick wherein said stick may be shortened by removing an end portion thereof which may have become contaminated by handling to a length fully receivable in said shortened package with said head in said growth media jelly in said first compartment -for resealing of said shortened package.
3. A kit according to Claim 2 wherein said film package has a second transverse weakening means along said second compartment for transversely tearing and reopening said resealed package to facilitate removal of said swab for testing of a culture thereon.
4. A kit according to Claim 2 wherein an identification label facilitates the resealing of said film package.
5. A kit according to Claim 2 wherein an identi-fication label facilitates the resealing of said film package, said label being carried by a second removable end portion of said film package and being retainable with said swab for identification purposes during culture testing.
6. A kit according to Claim 2 wherein said first rupturable seal is between two transverse permanent seal portions protecting into said package between said compartments, said transverse permanent seal portions defining a tapered guide at the end of said second com-partment adjacent said first compartment for guiding said swab head against said rupturable transverse seal to facilitate rupture of said transverse seal and movement of said swab head into said first compartment.
7. A method of taking a culture sample com-prising the steps of providing a two compartment film package including a first compartment containing a growth medium jelly and second compartment having therein a removable swab including an elongated stick with said compartments being in axially adjacent and aligned relation, and wherein said film package has a transverse rupturable seal between said compartments and an axially peelable seal at the end of said film package remote from said first compartment, opening said film package by rupturing said peelable seal and thereby exposing an end portion only of said stick, grasping only said stick end portion, removing said swab from said second compartment, utilizing said swab to obtain a culture sample, retrieving said swab into said second compartment and then through said rupturable seal into said first compartment to place the culture sample in said growth medium, breaking off said stick end portion of said film package between a new end of said stick and said peelable seal, and then resealing said film package.
8. The method of Claim 7 wherein said film package is resealed by folding over the new end of said film package, and then applying a resealing member.
9. The method of Claim 7 wherein said film package is resealed by folding over the new end of said film package, and then applying a resealing member in the form of a label.
10. The method of Claim 9 wherein the resealed film package is reopened at a test facility by tearing said film package transversely across said second compart-ment to separate a free end portion of said second com-partment including said label from the remainder of said resealed film package, and using said free end portion to remove and handle said swab.
CA000362110A 1979-10-11 1980-10-10 Culture swab kit and method of utilizing same Expired CA1156542A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US8398679A 1979-10-11 1979-10-11
US083,986 1979-10-11

Publications (1)

Publication Number Publication Date
CA1156542A true CA1156542A (en) 1983-11-08

Family

ID=22181918

Family Applications (1)

Application Number Title Priority Date Filing Date
CA000362110A Expired CA1156542A (en) 1979-10-11 1980-10-10 Culture swab kit and method of utilizing same

Country Status (7)

Country Link
JP (1) JPS5655198A (en)
AU (1) AU539613B2 (en)
CA (1) CA1156542A (en)
DE (1) DE3038282A1 (en)
GB (1) GB2059992B (en)
SE (1) SE8007078L (en)
ZA (1) ZA806109B (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0155747A1 (en) * 1984-03-19 1985-09-25 Kidde, Inc. Tube culture system
US4586604A (en) * 1984-06-28 1986-05-06 Continental Plastic Corporation Culture collection instrument and sealed swab holder therefor
IT1272598B (en) * 1993-09-09 1997-06-26 Copan Italia Spa DEVICE FOR THE COLLECTION AND TRANSPORT OF SAMPLES IN VITRO MAINLY FOR DIAGNOSTIC USE
US6383804B1 (en) * 2000-07-13 2002-05-07 International Bioproducts, Inc. Sampling device with snap-off head and method of use
CA2337282C (en) * 2001-03-05 2006-01-03 Victor Daykin Biological specimen collection apparatus
WO2004061418A2 (en) * 2002-12-26 2004-07-22 Meso Scale Technologies, Llc. Assay cartridges and methods of using the same
DE602004025381D1 (en) 2003-12-23 2010-03-18 Zhendong Wu PUNCH AND MANUFACTURING METHOD THEREFOR
JP5619338B2 (en) * 2007-03-19 2014-11-05 株式会社日研生物医学研究所 Environmental microbe wipe inspection tool
EP2171420A1 (en) 2007-07-31 2010-04-07 Micronics, Inc. Sanitary swab collection system, microfluidic assay device, and methods for diagnostic assays

Also Published As

Publication number Publication date
ZA806109B (en) 1981-09-30
AU539613B2 (en) 1984-10-11
GB2059992A (en) 1981-04-29
GB2059992B (en) 1983-10-19
JPS5655198A (en) 1981-05-15
SE8007078L (en) 1981-04-12
DE3038282A1 (en) 1981-04-23
AU6319180A (en) 1981-04-16

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