CA1129324A - Device for detecting microorganisms - Google Patents
Device for detecting microorganismsInfo
- Publication number
- CA1129324A CA1129324A CA352,329A CA352329A CA1129324A CA 1129324 A CA1129324 A CA 1129324A CA 352329 A CA352329 A CA 352329A CA 1129324 A CA1129324 A CA 1129324A
- Authority
- CA
- Canada
- Prior art keywords
- nutrient medium
- container
- closure means
- solid
- liquid nutrient
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
Landscapes
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
ABSTRACT
There is described an apparatus for the detection of microorganisms. The apparatus contains a first container with closure means for a liquid nutrient medium and a transport container with closure means and carrier fixed thereon for at least one solid nutrient medium. The closure means of the first container and of the transport container are interchangeable. When the closure means are interchanged, the solid nutrient medium does not extend into the liquid nutrient medium.
There is described an apparatus for the detection of microorganisms. The apparatus contains a first container with closure means for a liquid nutrient medium and a transport container with closure means and carrier fixed thereon for at least one solid nutrient medium. The closure means of the first container and of the transport container are interchangeable. When the closure means are interchanged, the solid nutrient medium does not extend into the liquid nutrient medium.
Description
1:~2932~ -The present invention is ccncerned with an apparatus for the detection of microorganisms, for example in body fluids.
For the detection of microorganisms in body fluids, especially of bacteria in blood, it is necessary to inoculate a li~uid nutrient medium and subsequently to continue the growth on a solid nutrient medium. For years there has existed a system in which both nutrient media are combined in the same container in order to avoid the troublesome and, under certain circumstances, risky transference of the pre-culture in the liquid nutrient medium on to a solid nutrient medium outside the container.
Most of the known apparatuses in which a solid and a liquid nutrient medium are combined are, however, not satisfactory for many reasons. In particular, because of the danger of the dissolving-out of constituents of the solid medium into the liquid nutrient medium, only a solid nutrient medium which is compatible with the liquid nutrient medium can generally be used. This has, inter alia, the considerable disadvantage that no differentiation of the microorganism is possible.
Klt/15.4.80 .
1~293~4 The hitherto proposed solutions for the separation of the solid nutrient medium and the liquid nutrient medium during the incubation period and the transport are time--comsuming and expensive and/or guarantee a separation of the two media only during the incubation, but not during the transpor~.
In DOS No. 28 06 ~02 there is proposed a system which does not have the aforementioned disadvantages. This system consists of a first container containing a liquid nutrient medium and a second container containing a solid nutrient medium, the interiors of the two containers being in contact and these containers being connected so that they can be separated again.
Figure 3 of the aforementioned DOS shows the combination of the two containers. The apparatus formed in this manner, which contains at least one solid and one liquid nutrient medium, is tilted several times in order to guarantee an optimum contact between the two nutrient media. After incubating at 20-37C for 1 hour to 10 days, any growth present on the solid nutrient medium is observed and evaluated. If no growth is ascertainable, this procedure can be repeated several times. In the case of longer incubation periods, the apparatus is tilted at least daily in order to guarantee an optimum contact between the li~uid and the solid nutrient medium.
.
It can be seen from the aforementioned Figure that the container containing the solid nutrient medium is connected with the container containing the liquid nutrient medium by means of a screw closure. In order to remove the container containing the solid nutrient medium (e.g. for the subsequent transport to the laboratory to determine the microorganisms on the solid nutrient medium), this screw closure must be unscrewed. In so doing, the danger may exist of liquid nutrient medium still present in the upper container (as a consequence of the aforementioned tiltings) running down the glass wall and wetting the tread. This may have the consequence that the person who separates the containers may come into contact with this liquid nutrient medium, which is to be avoided in all events.
A further critical feature of the apparatus according to DOS No. 28 06 902 is the inside thread 12 of the upper container shown in Figure 3. As is indeed known, inside threads are much more expensive to manufacture than outside - threads.
In accordance with the present invention there is provided an apparatus for the determination of microorganisms which is optimal in every respect.
More particularly, the present invention is concerned with an apparatus for the detection of microorganisms, consisting of a first container with closure means for a liquid nutrient medium and a transport container with closure means and carrier fixed thereon for at least one solid nutrient medium, the closure means of the first container and of the transport container being interchangeable.
In order that the present invention may be readily understood, one embodiment of the apparatus provided by the invention will now be described with reference to the accompanying drawings in which:
Figure 1 shows schematically the first container with closure means for a liquid nutrient medium;
Figure 2 shows schematically the transport container with closure means and carrier fixed thereon for at least one solid nutrient medium;
Figure 3 shows schematically the two closure mea~s.
Figure 4 shows schematically the combination of the container of Figure 1 with the closure means and solid carrier rixed thereon of the transport container OL Figure 2.
The first container for the liquid nutrient medium consists of a flask 1 which is preferably made from a transparent material such as glass or synthetic material.
The flask is closed by a screw cap 2. The screw cap carries , llZ9324 the inside thread 3, whereas the container has an outside thread 4. The screw cap is provided with means which allow a body fluid such as blood to be introduced into the flask with a needle (not shown in the Figure). The transport container 5 consists of a transparent tube of glass or synthetic material. A carrier 7 (e.g. z slide~
which is provided for at least one solid nutrient medium, is fixed to the screw cap 6 of the transport container.
The screw cap has an inside thread 8, whereas the transport container 5 has an outside thread 9.
It will be noted that the inside threads 3 and 8 as well as the outside threads 4 and ~ are identical.
In use, blood from a patient is conducted into the container 1 with the aid of a transfer instrument provided with a needle. The container which is provided with liquid nutrient medium 10 is under a slight vacuum in order to facilitate the flow of blood into the container.
When the container contains the desired amount of blood, incubation is carried out at 20-37C for about 1 hour to about 10 days.
Subsequently, the screw caps 2 and 6 are interchanged, whereby the apparatus shown in Figure 4 is obtained. The apparatus formed in this manner, containing at least one .
.
~ ~293Z4 solid and one liquid nutrient medium, is tilted several t:Lmes in order to guarantee an optimum contact between the two nutrient media. After incubation at 20-37C for l hour to 10 days, any growth present on the solid nutrient medium is observed. In order to evaluate the growth on the solid nutrient medium, the screw caps 2 and 6 are again interchanged.
The transport container with the solid nutrient medium can then be sent without difficulty to the laboratory for further investigation.
It will be appreciated that the present invention is not limited to the specific embodiment described hereinbefore.
Thus, for example, in the scope of the present invention the two containers 1 and 5 can have an arbitrary ~orm and can be closed in a different manner. It is nevertheless important that in the apparatus shown in Figure 4 the solid nutrient medium does not extend into the liquid nutrient medium lO.
It is essential that the closure means of the container for the liquid nutrient medium and for the transport container are interchangeable.
Of course, screw closures need not be used exclusively for the closure of the containers. The two containers can also be connected, for example, by bayonet closures.
.,, .~
For the detection of microorganisms in body fluids, especially of bacteria in blood, it is necessary to inoculate a li~uid nutrient medium and subsequently to continue the growth on a solid nutrient medium. For years there has existed a system in which both nutrient media are combined in the same container in order to avoid the troublesome and, under certain circumstances, risky transference of the pre-culture in the liquid nutrient medium on to a solid nutrient medium outside the container.
Most of the known apparatuses in which a solid and a liquid nutrient medium are combined are, however, not satisfactory for many reasons. In particular, because of the danger of the dissolving-out of constituents of the solid medium into the liquid nutrient medium, only a solid nutrient medium which is compatible with the liquid nutrient medium can generally be used. This has, inter alia, the considerable disadvantage that no differentiation of the microorganism is possible.
Klt/15.4.80 .
1~293~4 The hitherto proposed solutions for the separation of the solid nutrient medium and the liquid nutrient medium during the incubation period and the transport are time--comsuming and expensive and/or guarantee a separation of the two media only during the incubation, but not during the transpor~.
In DOS No. 28 06 ~02 there is proposed a system which does not have the aforementioned disadvantages. This system consists of a first container containing a liquid nutrient medium and a second container containing a solid nutrient medium, the interiors of the two containers being in contact and these containers being connected so that they can be separated again.
Figure 3 of the aforementioned DOS shows the combination of the two containers. The apparatus formed in this manner, which contains at least one solid and one liquid nutrient medium, is tilted several times in order to guarantee an optimum contact between the two nutrient media. After incubating at 20-37C for 1 hour to 10 days, any growth present on the solid nutrient medium is observed and evaluated. If no growth is ascertainable, this procedure can be repeated several times. In the case of longer incubation periods, the apparatus is tilted at least daily in order to guarantee an optimum contact between the li~uid and the solid nutrient medium.
.
It can be seen from the aforementioned Figure that the container containing the solid nutrient medium is connected with the container containing the liquid nutrient medium by means of a screw closure. In order to remove the container containing the solid nutrient medium (e.g. for the subsequent transport to the laboratory to determine the microorganisms on the solid nutrient medium), this screw closure must be unscrewed. In so doing, the danger may exist of liquid nutrient medium still present in the upper container (as a consequence of the aforementioned tiltings) running down the glass wall and wetting the tread. This may have the consequence that the person who separates the containers may come into contact with this liquid nutrient medium, which is to be avoided in all events.
A further critical feature of the apparatus according to DOS No. 28 06 902 is the inside thread 12 of the upper container shown in Figure 3. As is indeed known, inside threads are much more expensive to manufacture than outside - threads.
In accordance with the present invention there is provided an apparatus for the determination of microorganisms which is optimal in every respect.
More particularly, the present invention is concerned with an apparatus for the detection of microorganisms, consisting of a first container with closure means for a liquid nutrient medium and a transport container with closure means and carrier fixed thereon for at least one solid nutrient medium, the closure means of the first container and of the transport container being interchangeable.
In order that the present invention may be readily understood, one embodiment of the apparatus provided by the invention will now be described with reference to the accompanying drawings in which:
Figure 1 shows schematically the first container with closure means for a liquid nutrient medium;
Figure 2 shows schematically the transport container with closure means and carrier fixed thereon for at least one solid nutrient medium;
Figure 3 shows schematically the two closure mea~s.
Figure 4 shows schematically the combination of the container of Figure 1 with the closure means and solid carrier rixed thereon of the transport container OL Figure 2.
The first container for the liquid nutrient medium consists of a flask 1 which is preferably made from a transparent material such as glass or synthetic material.
The flask is closed by a screw cap 2. The screw cap carries , llZ9324 the inside thread 3, whereas the container has an outside thread 4. The screw cap is provided with means which allow a body fluid such as blood to be introduced into the flask with a needle (not shown in the Figure). The transport container 5 consists of a transparent tube of glass or synthetic material. A carrier 7 (e.g. z slide~
which is provided for at least one solid nutrient medium, is fixed to the screw cap 6 of the transport container.
The screw cap has an inside thread 8, whereas the transport container 5 has an outside thread 9.
It will be noted that the inside threads 3 and 8 as well as the outside threads 4 and ~ are identical.
In use, blood from a patient is conducted into the container 1 with the aid of a transfer instrument provided with a needle. The container which is provided with liquid nutrient medium 10 is under a slight vacuum in order to facilitate the flow of blood into the container.
When the container contains the desired amount of blood, incubation is carried out at 20-37C for about 1 hour to about 10 days.
Subsequently, the screw caps 2 and 6 are interchanged, whereby the apparatus shown in Figure 4 is obtained. The apparatus formed in this manner, containing at least one .
.
~ ~293Z4 solid and one liquid nutrient medium, is tilted several t:Lmes in order to guarantee an optimum contact between the two nutrient media. After incubation at 20-37C for l hour to 10 days, any growth present on the solid nutrient medium is observed. In order to evaluate the growth on the solid nutrient medium, the screw caps 2 and 6 are again interchanged.
The transport container with the solid nutrient medium can then be sent without difficulty to the laboratory for further investigation.
It will be appreciated that the present invention is not limited to the specific embodiment described hereinbefore.
Thus, for example, in the scope of the present invention the two containers 1 and 5 can have an arbitrary ~orm and can be closed in a different manner. It is nevertheless important that in the apparatus shown in Figure 4 the solid nutrient medium does not extend into the liquid nutrient medium lO.
It is essential that the closure means of the container for the liquid nutrient medium and for the transport container are interchangeable.
Of course, screw closures need not be used exclusively for the closure of the containers. The two containers can also be connected, for example, by bayonet closures.
.,, .~
Claims (3)
1. An apparatus for the detection of microorganisms, consisting of a first container with closure means for a liquid nutrient medium and a transport container with closure means and carrier fixed thereon for at least one solid nutrient medium, the closure means of the first container and of the transport container being interchangeable.
2. An apparatus according to claim 1, wherein the closure means are screw closures.
3. An apparatus according to claim 1, wherein the closure means are bayonet closures.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CH575979 | 1979-06-20 | ||
| CH5759/79 | 1979-06-20 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1129324A true CA1129324A (en) | 1982-08-10 |
Family
ID=4298877
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA352,329A Expired CA1129324A (en) | 1979-06-20 | 1980-05-21 | Device for detecting microorganisms |
Country Status (7)
| Country | Link |
|---|---|
| EP (1) | EP0020961B1 (en) |
| JP (1) | JPS5816871B2 (en) |
| AT (2) | ATE1018T1 (en) |
| AU (1) | AU535330B2 (en) |
| CA (1) | CA1129324A (en) |
| DE (1) | DE3060409D1 (en) |
| ES (1) | ES251542Y (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5654194A (en) * | 1995-11-09 | 1997-08-05 | Infectech, Inc. | Method of identifying a nonparaffinophilic microorganism using various milieus and an associated apparatus |
| US5668010A (en) * | 1995-11-09 | 1997-09-16 | Infectech, Inc. | Method for determining the antimicrobial agent sensitivity of a nonparaffinophilic microorganism using various milieus and an associated apparatus |
| US5677169A (en) * | 1995-09-14 | 1997-10-14 | Infectech, Inc. | Method for determining the antimicrobial agent sensitivity of a nonparaffinophilic microorganism and an associated apparatus |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MX2022011932A (en) | 2020-03-26 | 2022-10-20 | Univ Kagawa Nat Univ Corp | Novel l-rhamnose isomerase. |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3589983A (en) * | 1968-12-11 | 1971-06-29 | Becton Dickinson Co | Culture bottle assembly |
| US3783104A (en) * | 1971-11-12 | 1974-01-01 | Wilson Pharm & Chem Corp | Testing and culturing transport system and method of making same |
| US3783106A (en) * | 1972-03-15 | 1974-01-01 | Wilson Pharm & Chem Corp | Testing and culturing transport system |
| CH625831A5 (en) * | 1977-02-18 | 1981-10-15 | Hoffmann La Roche | |
| FR2381103A1 (en) * | 1977-02-18 | 1978-09-15 | Pasteur Institut | Flask for cultivating biological culture partic. for blood tests - uses solid and liq. culture media simultaneously in controlled atmos. |
-
1980
- 1980-05-08 EP EP80102505A patent/EP0020961B1/en not_active Expired
- 1980-05-08 AT AT80102505T patent/ATE1018T1/en not_active IP Right Cessation
- 1980-05-08 DE DE8080102505T patent/DE3060409D1/en not_active Expired
- 1980-05-21 CA CA352,329A patent/CA1129324A/en not_active Expired
- 1980-06-13 AU AU59290/80A patent/AU535330B2/en not_active Ceased
- 1980-06-19 AT AT0324380A patent/ATA324380A/en not_active IP Right Cessation
- 1980-06-19 ES ES1980251542U patent/ES251542Y/en not_active Expired
- 1980-06-19 JP JP55082190A patent/JPS5816871B2/en not_active Expired
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5677169A (en) * | 1995-09-14 | 1997-10-14 | Infectech, Inc. | Method for determining the antimicrobial agent sensitivity of a nonparaffinophilic microorganism and an associated apparatus |
| US5654194A (en) * | 1995-11-09 | 1997-08-05 | Infectech, Inc. | Method of identifying a nonparaffinophilic microorganism using various milieus and an associated apparatus |
| US5668010A (en) * | 1995-11-09 | 1997-09-16 | Infectech, Inc. | Method for determining the antimicrobial agent sensitivity of a nonparaffinophilic microorganism using various milieus and an associated apparatus |
Also Published As
| Publication number | Publication date |
|---|---|
| DE3060409D1 (en) | 1982-07-01 |
| ATE1018T1 (en) | 1982-05-15 |
| EP0020961A1 (en) | 1981-01-07 |
| ES251542Y (en) | 1982-01-01 |
| ES251542U (en) | 1981-08-01 |
| JPS5816871B2 (en) | 1983-04-02 |
| JPS565090A (en) | 1981-01-20 |
| EP0020961B1 (en) | 1982-05-12 |
| AU5929080A (en) | 1981-01-08 |
| AU535330B2 (en) | 1984-03-15 |
| ATA324380A (en) | 1981-12-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MKEX | Expiry |