CA1042805A - Enzyme containing oral hygiene compositions - Google Patents
Enzyme containing oral hygiene compositionsInfo
- Publication number
- CA1042805A CA1042805A CA193,338A CA193338A CA1042805A CA 1042805 A CA1042805 A CA 1042805A CA 193338 A CA193338 A CA 193338A CA 1042805 A CA1042805 A CA 1042805A
- Authority
- CA
- Canada
- Prior art keywords
- fructan
- degrading enzyme
- composition
- enzyme
- dental
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/66—Enzymes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01065—Levanase (3.2.1.65)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Cosmetics (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
ABSTRACT
Compositions for oral hygiene, containing a fructan-degrading enzyme and an orally acceptable excipient, are useful for preventing the formation of and removing dental plaque. The enzyme can be incorporated into tooth-pastes, tooth-powders, mouth-washes, gargles, dental lotions and ointments, chewing-gums, troches, foodstuffs and beverages.
Compositions for oral hygiene, containing a fructan-degrading enzyme and an orally acceptable excipient, are useful for preventing the formation of and removing dental plaque. The enzyme can be incorporated into tooth-pastes, tooth-powders, mouth-washes, gargles, dental lotions and ointments, chewing-gums, troches, foodstuffs and beverages.
Description
This invention relates to compositions for oral ~ -hygiene.
It is currently believed that one factor in the form-ation of dental caries is the presence of food residues trapped in the interstices between the teeth, and in -pits and fissures inthe teeth themselves, it being theorized that carbohydrates in such food residues are bacterially decomposed to lactic acid whlch promotes de-calcification of dental material. The acid-producing bacteria are known to adhere firmly to the surface of the teeth as a thin film, giving rise to dental plaque.
The formation and adhesion to the teeth of dental plaque is also thought to be a precursor of tartar formation and of inflammations of the gum and periodontal tissue, such as pyorrhea alveolaris and periodontitis. Preventing the formation of dental plaque, or removing it, is there- ;
fore regarded as an important factor in oral hygiene.
It has now been found that a strain of Actinomyces viscosus isolated from human dental plaque proudces levan, -..
an exocellu~rfructan, int he presence of sucrose, and that the levan is implicated inthe formation and adhesion of the plaque on the surface of the teeth. Furthermore, it has been discovered that fructan-degrading enzymes can be used to help diminish the formation of dental plaque, or to assist in removing it from the teeth, and thus ~
improve oral hygiene. For this purpose, the invention pro-. :^ .: -.. .
vides compositions for dental and oral use, comprising a fructan-degrading enzyme and an orally acceptable excipient.
.'~ "' - '.
- 1 - ' " "'~:
.-- ~ - .. .. .. . . .. - ... , . ,, . ., - - ....... .. . .. .... .... . .
11)4Z8QS
According to one aspect, the compositions of the invention may take any of the forms normally intended for cleaning, antiseptic or emollient purposes in the mouth: for example, tooth-powders, tooth-pastes, mouth-washes, gargles, dental lotions and ointments, chewing-gums, tablets, and mouth or throat pastilles or troches.
These compositions of the invention comprise a fructan-degrading enzyme and an edible solid excipient, a dental detergent, an anti-septic, an emollient, a demulcent, or a chewing-gum base.
In accordance with another aspect of the invention, the fructan-degrading enzyme can be incorporated into an edible compo-sition, comprising a foodstuff or beverage. For example, the fructan-degrading enzyme can be incorporated into soft drinks or confectionery products, tn combat the formation of dental plaque otherwise resulting on eating these products.
The fructan-degrading enzyme used in the compositions of the invention is one produced by a micro-organism of the genus Talaromyces, ~upenicillium, Sporotrichum, Sordaria, Chaetomium, Gelasinospora, Stachybotris or Actinomyces, in accordance with the process of Canadian Patent No. 1,003,351. Although all fructan-degrading enzymes can be used in the compositions of the invention,it is preferable to use - . - . . . .
` 104Z8Q5 those of the liquefying type (i.e. endo-enzymes), which can chop up the levan molecule anywhere along its length, rather than those of the saccharifying type (i.e. exo-enzymes), which degrade it step-wise from the ends. Enzymes of the so-called "levanase type", which cut ~-(2 -~6) linkages in the main chain of the levan molecule and enzymes of the so-called "inulase type", which cut ~-(2 >1) linkages, are both effective in their own right; but it is even better to use a combination of two or more different types of highly active fructan-degrading enzyme.
In addition to containing one or more fructan-degrading enzymes, the compositions of the invention may optionally also con-tain one or more other enzymes, such as a dextranase (3.2.1.11), a-amylase (3.2.1.1), protease (3.4.4.16 and 3.4.4.17) or lipase J
(3.1.1.3) in order to supplement their dental cleaning activity.
The concentration of fructan-degrading enzyme used in the compositions of the invention can vary widely, depending upon the ~-type of composition, and hence on its expected residence time in the mouth and frequency of use. A unit dosage of the composition can generally contain from 1 to 3,000, preferably from 1 to 300 and ~0 most preferably from 10 to 100, activity units of fructan-degrading enzyme. The compositions should preferably be used several times daily, suitably with a total daily dosage corresponding to about 1,500 activity units of fructan-degrading enzyme. Naturally, it is preferable for the compositions to be retained in the mouth for as long as possible.
..... .
,, .
:
..
```'''~'`'.
- 3 - ~
~042805 The activity of the fructan-degrading enzyme is determined by adding it to l ml of M/20 citric acid buffer (pH 5.4) containing 0.5% by weight of fructan (from Aero-bacter le _nicum). The mixture is kept at 40 C for 30 minutes, to liberate reducing sugar, which is determined as fructose with 3,5-dinitrosalicylic acid. The"activity unit" of the enzyme is defined as that quantity which liberates 1 mg of fructose in 60 minutes.
The ingredients uscd in the compositions of the invention, apart from the enzyme or enzymes, will depend upon the precise form of composition. Any of the ingre-dients normally used for the types of formulation already listed may also be used in the compositions of the inven-tion; and, apart from ingredients such as the detergent, antiseptic, emollient or demulscent, or the chewing-gum base, the compositions may also contain conventionally used additives, such as flovouring agents, perfumes and sweetners. In general, the fructan-degrading enzyme may be formulated with any orally acceptable excipient, depending upon the intended form and use of the composition.
The beneficial effect of fructan-degrading enzyme in reducing the formation of dental plaque by oral micro-organisms can be demonstrated in the laboratory by the methods of Jordan et al. Archs. Oral Biol., 11 739 (1966) and of Onisi et al. Journal of Dental Health, Japan, 20 292 (1971). In order to simulate experimentally the in vivo formation of dental plaque, pure cultures were pre-pared using the -.. ~
:
'' :. ' following media and micro-organisms~
Med;a: (1) the basic medium of Jordan et al. J.
Dent. Res., 39, 116 (1960)
It is currently believed that one factor in the form-ation of dental caries is the presence of food residues trapped in the interstices between the teeth, and in -pits and fissures inthe teeth themselves, it being theorized that carbohydrates in such food residues are bacterially decomposed to lactic acid whlch promotes de-calcification of dental material. The acid-producing bacteria are known to adhere firmly to the surface of the teeth as a thin film, giving rise to dental plaque.
The formation and adhesion to the teeth of dental plaque is also thought to be a precursor of tartar formation and of inflammations of the gum and periodontal tissue, such as pyorrhea alveolaris and periodontitis. Preventing the formation of dental plaque, or removing it, is there- ;
fore regarded as an important factor in oral hygiene.
It has now been found that a strain of Actinomyces viscosus isolated from human dental plaque proudces levan, -..
an exocellu~rfructan, int he presence of sucrose, and that the levan is implicated inthe formation and adhesion of the plaque on the surface of the teeth. Furthermore, it has been discovered that fructan-degrading enzymes can be used to help diminish the formation of dental plaque, or to assist in removing it from the teeth, and thus ~
improve oral hygiene. For this purpose, the invention pro-. :^ .: -.. .
vides compositions for dental and oral use, comprising a fructan-degrading enzyme and an orally acceptable excipient.
.'~ "' - '.
- 1 - ' " "'~:
.-- ~ - .. .. .. . . .. - ... , . ,, . ., - - ....... .. . .. .... .... . .
11)4Z8QS
According to one aspect, the compositions of the invention may take any of the forms normally intended for cleaning, antiseptic or emollient purposes in the mouth: for example, tooth-powders, tooth-pastes, mouth-washes, gargles, dental lotions and ointments, chewing-gums, tablets, and mouth or throat pastilles or troches.
These compositions of the invention comprise a fructan-degrading enzyme and an edible solid excipient, a dental detergent, an anti-septic, an emollient, a demulcent, or a chewing-gum base.
In accordance with another aspect of the invention, the fructan-degrading enzyme can be incorporated into an edible compo-sition, comprising a foodstuff or beverage. For example, the fructan-degrading enzyme can be incorporated into soft drinks or confectionery products, tn combat the formation of dental plaque otherwise resulting on eating these products.
The fructan-degrading enzyme used in the compositions of the invention is one produced by a micro-organism of the genus Talaromyces, ~upenicillium, Sporotrichum, Sordaria, Chaetomium, Gelasinospora, Stachybotris or Actinomyces, in accordance with the process of Canadian Patent No. 1,003,351. Although all fructan-degrading enzymes can be used in the compositions of the invention,it is preferable to use - . - . . . .
` 104Z8Q5 those of the liquefying type (i.e. endo-enzymes), which can chop up the levan molecule anywhere along its length, rather than those of the saccharifying type (i.e. exo-enzymes), which degrade it step-wise from the ends. Enzymes of the so-called "levanase type", which cut ~-(2 -~6) linkages in the main chain of the levan molecule and enzymes of the so-called "inulase type", which cut ~-(2 >1) linkages, are both effective in their own right; but it is even better to use a combination of two or more different types of highly active fructan-degrading enzyme.
In addition to containing one or more fructan-degrading enzymes, the compositions of the invention may optionally also con-tain one or more other enzymes, such as a dextranase (3.2.1.11), a-amylase (3.2.1.1), protease (3.4.4.16 and 3.4.4.17) or lipase J
(3.1.1.3) in order to supplement their dental cleaning activity.
The concentration of fructan-degrading enzyme used in the compositions of the invention can vary widely, depending upon the ~-type of composition, and hence on its expected residence time in the mouth and frequency of use. A unit dosage of the composition can generally contain from 1 to 3,000, preferably from 1 to 300 and ~0 most preferably from 10 to 100, activity units of fructan-degrading enzyme. The compositions should preferably be used several times daily, suitably with a total daily dosage corresponding to about 1,500 activity units of fructan-degrading enzyme. Naturally, it is preferable for the compositions to be retained in the mouth for as long as possible.
..... .
,, .
:
..
```'''~'`'.
- 3 - ~
~042805 The activity of the fructan-degrading enzyme is determined by adding it to l ml of M/20 citric acid buffer (pH 5.4) containing 0.5% by weight of fructan (from Aero-bacter le _nicum). The mixture is kept at 40 C for 30 minutes, to liberate reducing sugar, which is determined as fructose with 3,5-dinitrosalicylic acid. The"activity unit" of the enzyme is defined as that quantity which liberates 1 mg of fructose in 60 minutes.
The ingredients uscd in the compositions of the invention, apart from the enzyme or enzymes, will depend upon the precise form of composition. Any of the ingre-dients normally used for the types of formulation already listed may also be used in the compositions of the inven-tion; and, apart from ingredients such as the detergent, antiseptic, emollient or demulscent, or the chewing-gum base, the compositions may also contain conventionally used additives, such as flovouring agents, perfumes and sweetners. In general, the fructan-degrading enzyme may be formulated with any orally acceptable excipient, depending upon the intended form and use of the composition.
The beneficial effect of fructan-degrading enzyme in reducing the formation of dental plaque by oral micro-organisms can be demonstrated in the laboratory by the methods of Jordan et al. Archs. Oral Biol., 11 739 (1966) and of Onisi et al. Journal of Dental Health, Japan, 20 292 (1971). In order to simulate experimentally the in vivo formation of dental plaque, pure cultures were pre-pared using the -.. ~
:
'' :. ' following media and micro-organisms~
Med;a: (1) the basic medium of Jordan et al. J.
Dent. Res., 39, 116 (1960)
(2) the basic medium (1) containing 10 activity units per 10 ml of fructan-degrading enzyme (from Talaromyces flavus var. flavus) Micro-organism: (1) Actinomyces viscosus, Onisi No. 39 ;~
strain, isolated from human dental plaque (similar to Actinomyces viscosus from rats and hamsters).
(2) Actinomyces viscosus, Onisi No.42 strain, (isolated as above).
Test-tubes containing 10 ml portions of the above mèdia, which were freshly prepared daily, were sub-cultured with 0.2 ml of cultures of the above micro-orgaisms, and each tube was fitted with a rubber stopper carrying a number 20 gauge stainless-steel wire which dipped into the medium.
This procédure was repeated for 10 days, and the dental ~ ...... .. .
plaque sticking to the stainless-steel wire was observed -`
with the naked eye. It was found that the f~`rmation of dental plaque in the portions of medium (2) containing fructan-degrading enzyme was greatly inhibited by com-parison~,with the basic medium (1). Moreover, when the wire carrying the dental plaque formed in the basic med-ium (1) was dipped into a solution containing 10 activity units/nl of fructan-degrading enzyme, the plaque came off -the wire in a few hours; while no change was observed when the wire carrying dental plaque was dipped into plain water, or into a solution of the enzyme inactivated by heating. These experiments demomstrate the effectiveness ;
of the fructan-degrading enzyme for preventing the '~ ~
,... -.
~ 5 - ~ ~ ' . . . . . . . .. .. .. .. ,. . . ~ ~ , ... . . .. ' ' ' -104Z8~5 formation of dental plaque and for removing it.
The invention is illustrated by the followng Examples, in which percentages are given by weight and the "activity units" are as hereinbefore defined.
Example 1 Tooth-paste Dibasic calcium phosphate 50%
Glycerol 20%
Sodium lauryl sulphate 2.5%
Spearmint oil 2.5%
Gum tragacanth 1.0%
Saccharin 0.1%
23 9%
Water A fructan-degrading enzyme, derived from Talaromyces flavus var. flavus, was incorporated into a tooth-paste having the above composition, at the rate of 10 activity units per gram of the paste, and the mixture was uniformly blended to form an enzyme-containing tooth-paste. ~ -:!1 - .
Example 2 Mouth-wash Sodium carboxymethylcellulose 4.0%
Sodium lauryl sulphate 2.0%
:
Glycerol 104Z8~5 30%
Perfume 0.5%
Water 63.5%
' "~-A fructan-degrading enzyme, derived from Eupenicillium javanic _ , was incorporated into a mouth-wash having the above composition, at the rate of 10 activity units per gram of the wash, and was uniformly dissolved therein to obtain an enzyme-containing mouth-wash.
Example 3 :::
Chewing-gum ~ :
Polyvinyl acetate 20% -Butyl phthalylbutylglycolate 3%
. ....... .... .
Polyisobutylene 3%
Microcrystalline wax 2%
Calcium carbonate 2%
Glucose 69%
Perfume 1%
A mixture of fructan-degrading enzymes, derived from Chaetomium subspirale and Eupenicillium javanicum, was incorporated into a chewing-gum base having the above comp-osition, at the rate of 10 activity units per gram of the gum, and the mixture was uniformly blended to form an :
enzyme-containing chewing gum. I
: ' .
. ...
, .:
........
:' , ' ' .,:: '.
'`'' " ' ' "'''"
Example 4 1~4~S
Chewable tablets Mannitol 65~
Soluble starch 30%
"Avicel" microcrystalline cellulose (a trademark of Asahi Chemical Industry Co.
Ltd., Japan) 2.5%
Food pigment trace "Aerosil 200" colloidal silicon dioxide (a trademark of Degussa, Germany) 0.6%
Magnesium stearate 0.6%
Saccharin 0.8%
"Orange-microne" perfume (a trademark of Takasago Perfumery Co. Ltd., Japan)0.3%
A mixture of fructan-degrading enzymes, derived from Sordaria humana and Talaromyces flavus var. flavus, was incorporated into the above chewable tablet base, at the rate of l00 activity units per gram. The mixture was uniformly blended and tabulated, to produce enzyme-containing chewable tablets.
~
.~' . ' ' - .:
strain, isolated from human dental plaque (similar to Actinomyces viscosus from rats and hamsters).
(2) Actinomyces viscosus, Onisi No.42 strain, (isolated as above).
Test-tubes containing 10 ml portions of the above mèdia, which were freshly prepared daily, were sub-cultured with 0.2 ml of cultures of the above micro-orgaisms, and each tube was fitted with a rubber stopper carrying a number 20 gauge stainless-steel wire which dipped into the medium.
This procédure was repeated for 10 days, and the dental ~ ...... .. .
plaque sticking to the stainless-steel wire was observed -`
with the naked eye. It was found that the f~`rmation of dental plaque in the portions of medium (2) containing fructan-degrading enzyme was greatly inhibited by com-parison~,with the basic medium (1). Moreover, when the wire carrying the dental plaque formed in the basic med-ium (1) was dipped into a solution containing 10 activity units/nl of fructan-degrading enzyme, the plaque came off -the wire in a few hours; while no change was observed when the wire carrying dental plaque was dipped into plain water, or into a solution of the enzyme inactivated by heating. These experiments demomstrate the effectiveness ;
of the fructan-degrading enzyme for preventing the '~ ~
,... -.
~ 5 - ~ ~ ' . . . . . . . .. .. .. .. ,. . . ~ ~ , ... . . .. ' ' ' -104Z8~5 formation of dental plaque and for removing it.
The invention is illustrated by the followng Examples, in which percentages are given by weight and the "activity units" are as hereinbefore defined.
Example 1 Tooth-paste Dibasic calcium phosphate 50%
Glycerol 20%
Sodium lauryl sulphate 2.5%
Spearmint oil 2.5%
Gum tragacanth 1.0%
Saccharin 0.1%
23 9%
Water A fructan-degrading enzyme, derived from Talaromyces flavus var. flavus, was incorporated into a tooth-paste having the above composition, at the rate of 10 activity units per gram of the paste, and the mixture was uniformly blended to form an enzyme-containing tooth-paste. ~ -:!1 - .
Example 2 Mouth-wash Sodium carboxymethylcellulose 4.0%
Sodium lauryl sulphate 2.0%
:
Glycerol 104Z8~5 30%
Perfume 0.5%
Water 63.5%
' "~-A fructan-degrading enzyme, derived from Eupenicillium javanic _ , was incorporated into a mouth-wash having the above composition, at the rate of 10 activity units per gram of the wash, and was uniformly dissolved therein to obtain an enzyme-containing mouth-wash.
Example 3 :::
Chewing-gum ~ :
Polyvinyl acetate 20% -Butyl phthalylbutylglycolate 3%
. ....... .... .
Polyisobutylene 3%
Microcrystalline wax 2%
Calcium carbonate 2%
Glucose 69%
Perfume 1%
A mixture of fructan-degrading enzymes, derived from Chaetomium subspirale and Eupenicillium javanicum, was incorporated into a chewing-gum base having the above comp-osition, at the rate of 10 activity units per gram of the gum, and the mixture was uniformly blended to form an :
enzyme-containing chewing gum. I
: ' .
. ...
, .:
........
:' , ' ' .,:: '.
'`'' " ' ' "'''"
Example 4 1~4~S
Chewable tablets Mannitol 65~
Soluble starch 30%
"Avicel" microcrystalline cellulose (a trademark of Asahi Chemical Industry Co.
Ltd., Japan) 2.5%
Food pigment trace "Aerosil 200" colloidal silicon dioxide (a trademark of Degussa, Germany) 0.6%
Magnesium stearate 0.6%
Saccharin 0.8%
"Orange-microne" perfume (a trademark of Takasago Perfumery Co. Ltd., Japan)0.3%
A mixture of fructan-degrading enzymes, derived from Sordaria humana and Talaromyces flavus var. flavus, was incorporated into the above chewable tablet base, at the rate of l00 activity units per gram. The mixture was uniformly blended and tabulated, to produce enzyme-containing chewable tablets.
~
.~' . ' ' - .:
Claims (6)
PROPERTY OR PRIVILEGE IS CLAIMED ARE DEFINED AS FOLLOWS:
1. A composition for oral hygiene, comprising at least one fructan-degrading enzyme incorporated in an orally acceptable excipient, the enzyme being present in an amount of from 1 to 3,000 activity units per unit dose of the composition and being derived from a micro-organism belonging to a genus selected from the group consisting of Talaromyces, Eupenicillium, Sporotrichum, Sordaria, Chaetomium, Gelasinospora, Stachybotris or Actinomyces.
2. A composition according to claim 1, wherein said fructan-degrading enzyme is incorporated in a formulation selected from the group consisting of tooth-powders, tooth-pastes, mouth-washes, gargles, dental ointments, dental lotions, tablets, troches and chewing-gums.
3. An edible composition comprising at least one fructan-degrading enzyme and an edible carrier selected from the group consisting of foodstuffs and beverages.
4. A composition according to claim 1, 2 or 3, which comprises from 1 to 300 activity units of fructan-degrading enzyme per unit dose of composition.
5. A composition according to claim 1, claim 2 or claim 3, which comprises from 10 to 100 activity units of fructan degrading enzyme per unit dose of composition.
6. A composition according to claim 1, 2 or 3, which includes at least one enzyme selected from the group consisting of dextranase (3.2.1.11), ? amylase (3.2.1.1), proteases (3.4.4.
16 and 3.4.4.17) and lipase (3.1.1.3).
16 and 3.4.4.17) and lipase (3.1.1.3).
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2284273A JPS5623968B2 (en) | 1973-02-26 | 1973-02-26 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1042805A true CA1042805A (en) | 1978-11-21 |
Family
ID=12093948
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA193,338A Expired CA1042805A (en) | 1973-02-26 | 1974-02-25 | Enzyme containing oral hygiene compositions |
Country Status (9)
| Country | Link |
|---|---|
| JP (1) | JPS5623968B2 (en) |
| CA (1) | CA1042805A (en) |
| CH (1) | CH594411A5 (en) |
| DE (1) | DE2408997A1 (en) |
| DK (1) | DK136201B (en) |
| FR (1) | FR2218874B1 (en) |
| GB (1) | GB1420529A (en) |
| NL (1) | NL7402609A (en) |
| SE (1) | SE420265B (en) |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE1927411A1 (en) * | 1969-05-28 | 1970-12-10 | Versuchs & Lehranstalt | Dental prepn for dental hygiene and removal - of dental films |
| DE1938189A1 (en) * | 1969-07-28 | 1971-02-25 | Blendax Werke Schneider Co | Dental and oral care products containing enzymes |
| BE755331A (en) * | 1969-09-01 | 1971-02-01 | Blendax Werke Schneider Co | ORAL AND DENTAL CARE PRODUCTS |
| US3751561A (en) * | 1970-11-23 | 1973-08-07 | Monsanto Co | Stable polymer-enzyme oral hygiene compositions |
-
1973
- 1973-02-26 JP JP2284273A patent/JPS5623968B2/ja not_active Expired
-
1974
- 1974-02-19 GB GB754874A patent/GB1420529A/en not_active Expired
- 1974-02-21 CH CH247474A patent/CH594411A5/xx not_active IP Right Cessation
- 1974-02-21 SE SE7402299A patent/SE420265B/en not_active IP Right Cessation
- 1974-02-25 DK DK99374A patent/DK136201B/en not_active IP Right Cessation
- 1974-02-25 CA CA193,338A patent/CA1042805A/en not_active Expired
- 1974-02-25 DE DE19742408997 patent/DE2408997A1/en not_active Ceased
- 1974-02-26 NL NL7402609A patent/NL7402609A/xx not_active Application Discontinuation
- 1974-02-26 FR FR7406527A patent/FR2218874B1/fr not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| FR2218874B1 (en) | 1978-09-15 |
| DK136201C (en) | 1978-02-27 |
| JPS5623968B2 (en) | 1981-06-03 |
| DE2408997A1 (en) | 1974-09-05 |
| FR2218874A1 (en) | 1974-09-20 |
| JPS49109544A (en) | 1974-10-18 |
| CH594411A5 (en) | 1978-01-13 |
| SE420265B (en) | 1981-09-28 |
| GB1420529A (en) | 1976-01-07 |
| DK136201B (en) | 1977-09-05 |
| NL7402609A (en) | 1974-08-28 |
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