CA1042439A - Bis-basic ethers of 9-substituted phenanthrene and 10-oxa and 10-aza derivatives thereof - Google Patents
Bis-basic ethers of 9-substituted phenanthrene and 10-oxa and 10-aza derivatives thereofInfo
- Publication number
- CA1042439A CA1042439A CA184,541A CA184541A CA1042439A CA 1042439 A CA1042439 A CA 1042439A CA 184541 A CA184541 A CA 184541A CA 1042439 A CA1042439 A CA 1042439A
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- formula
- fluoren
- group
- ethoxy
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D295/00—Heterocyclic compounds containing polymethylene-imine rings with at least five ring members, 3-azabicyclo [3.2.2] nonane, piperazine, morpholine or thiomorpholine rings, having only hydrogen atoms directly attached to the ring carbon atoms
- C07D295/04—Heterocyclic compounds containing polymethylene-imine rings with at least five ring members, 3-azabicyclo [3.2.2] nonane, piperazine, morpholine or thiomorpholine rings, having only hydrogen atoms directly attached to the ring carbon atoms with substituted hydrocarbon radicals attached to ring nitrogen atoms
- C07D295/08—Heterocyclic compounds containing polymethylene-imine rings with at least five ring members, 3-azabicyclo [3.2.2] nonane, piperazine, morpholine or thiomorpholine rings, having only hydrogen atoms directly attached to the ring carbon atoms with substituted hydrocarbon radicals attached to ring nitrogen atoms substituted by singly bound oxygen or sulfur atoms
- C07D295/084—Heterocyclic compounds containing polymethylene-imine rings with at least five ring members, 3-azabicyclo [3.2.2] nonane, piperazine, morpholine or thiomorpholine rings, having only hydrogen atoms directly attached to the ring carbon atoms with substituted hydrocarbon radicals attached to ring nitrogen atoms substituted by singly bound oxygen or sulfur atoms with the ring nitrogen atoms and the oxygen or sulfur atoms attached to the same carbon chain, which is not interrupted by carbocyclic rings
- C07D295/088—Heterocyclic compounds containing polymethylene-imine rings with at least five ring members, 3-azabicyclo [3.2.2] nonane, piperazine, morpholine or thiomorpholine rings, having only hydrogen atoms directly attached to the ring carbon atoms with substituted hydrocarbon radicals attached to ring nitrogen atoms substituted by singly bound oxygen or sulfur atoms with the ring nitrogen atoms and the oxygen or sulfur atoms attached to the same carbon chain, which is not interrupted by carbocyclic rings to an acyclic saturated chain
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D295/00—Heterocyclic compounds containing polymethylene-imine rings with at least five ring members, 3-azabicyclo [3.2.2] nonane, piperazine, morpholine or thiomorpholine rings, having only hydrogen atoms directly attached to the ring carbon atoms
- C07D295/04—Heterocyclic compounds containing polymethylene-imine rings with at least five ring members, 3-azabicyclo [3.2.2] nonane, piperazine, morpholine or thiomorpholine rings, having only hydrogen atoms directly attached to the ring carbon atoms with substituted hydrocarbon radicals attached to ring nitrogen atoms
- C07D295/08—Heterocyclic compounds containing polymethylene-imine rings with at least five ring members, 3-azabicyclo [3.2.2] nonane, piperazine, morpholine or thiomorpholine rings, having only hydrogen atoms directly attached to the ring carbon atoms with substituted hydrocarbon radicals attached to ring nitrogen atoms substituted by singly bound oxygen or sulfur atoms
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D405/00—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom
- C07D405/02—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings
- C07D405/12—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings linked by a chain containing hetero atoms as chain links
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C2603/00—Systems containing at least three condensed rings
- C07C2603/02—Ortho- or ortho- and peri-condensed systems
- C07C2603/04—Ortho- or ortho- and peri-condensed systems containing three rings
- C07C2603/06—Ortho- or ortho- and peri-condensed systems containing three rings containing at least one ring with less than six ring members
- C07C2603/10—Ortho- or ortho- and peri-condensed systems containing three rings containing at least one ring with less than six ring members containing five-membered rings
- C07C2603/12—Ortho- or ortho- and peri-condensed systems containing three rings containing at least one ring with less than six ring members containing five-membered rings only one five-membered ring
- C07C2603/18—Fluorenes; Hydrogenated fluorenes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C2603/00—Systems containing at least three condensed rings
- C07C2603/02—Ortho- or ortho- and peri-condensed systems
- C07C2603/04—Ortho- or ortho- and peri-condensed systems containing three rings
- C07C2603/22—Ortho- or ortho- and peri-condensed systems containing three rings containing only six-membered rings
- C07C2603/26—Phenanthrenes; Hydrogenated phenanthrenes
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
Abstract
ABSTRACT OF THE DISCLOSURE
Novel bis-basic ethers of 9-substituted phenanthrene and related 10-oxa and 10-aza derivatives, their method of preparation and their use as pharmaceutical agents for the prevention and inhibition of viral infections are disclosed.
Novel bis-basic ethers of 9-substituted phenanthrene and related 10-oxa and 10-aza derivatives, their method of preparation and their use as pharmaceutical agents for the prevention and inhibition of viral infections are disclosed.
Description
~4Z439 FIELD OF THE INVENTION
; This invention relates to new organic chemical com- pounds, to their preparation and to pharmaceutical compo-sitions çontaining such compounds. The compounds de-scribed herein are useful antiviral agents which inhibit or inactivate viruses by their administration to either an infected qr to a non-infected host.
~-BACKGROUND OF THE INVENTION
There is a growing body of information that viruses play a vital role in a broad range of diseases, some of which represent the most serious of man's ills. Arthritis~
juvenile arthritis, diabetes, Hodgkin's disease and various immunological diseases and degenerative diseases of the central nervous system have been linked to viruses as the causative agents.
At present, the control of virus infecttons is primarily achieved by means of immunization vacc;nes.
' ~
- WJS:dw . 1-.
~ 39 For examp 1 e, po 1 i omye 1 i tisl sma 1 1 pox, meas 1 es and i nf 1 uenza are well recognized diseases in which viral vaccines have proven effective. In general, however, viral vaccines have had only a moderate success in animal prophylaxis.
Each vaccine acts primarily against a specific virus and is not heterophilic in the protection it offers. Hence~
vaccines do not provide a practical solution against the wide array of infectious viruses, even where limitedg as for example, solely to respiratory viruses.
One approach to the control of virus-related diseases and, particularly to the spread of such virus diseases, ;
has been to search for mediclnal agents or chemothera- -peutic agents which are capable of inhibiting the growth of viruses, thereby preventing the spread of disease as well as preventing further damage to cells and tissues ~`
of the animal host which have not as yet been infectedO ;
Heretoforeg only a limited number of virus infections such as smallpox, Asian influenza and herpes~keratitis have been susceptible to prevention by chemical antiviral agents. Sulfonamides and ant;biotics which hàve revo--lutionized the treatment of bacterial infections have substantially no effect upon virus infections. Certain infections caused by large v;ruses, such as iymphogranuloma venereum, psittacosis and trachoma have been successfully treated using antibiotics and sulfa drugs. However~ the majority of infections have not been respons;ve to attack by chemotherapeutic agents. Thus, it can be seen that there is a need for new chemotherapeutic agents which are effective against a broad range of virus diseases9 and which at the same time, are non-toxic to the;host.
: ., ~' ' - : ' - :
: .' . ' , , :
Canada ~ ~ Z ~ 39 As a result of a long series of investigations, applicants have discovered a novel class of antiviral agents which are bis-basic ether derivatives of 9-sub-stituted phenanthrene and which also include their corresponding 10-oxa and 10-aza derivatives. A more descriptive designation for these compounds in accord-ance with Chemical Abstracts nomenclature would be to describe them as bis-basic ether derivatives of 9-phenan-throl, 6(5H)-phenanthridinone and 6H-dibenzo[b,d]pyran-10 6-one. These compounds are effective against a wide spectrum of viral infections and are useful in treating such infections either prophylactically or therapeutically.
U.S. Patent 3,592,819 is the closest art known to applicants and discloses certain bis-basic ethers and thioethers of various substituted fluorenesJ 9-fluorenols and 9-fluorenones useful as antiviral agents. Certain of the bis-basic ethers described therein serve as starting materials for the preparation of some of the compounds of the present invention.
The bis-basic ethers described and claimed herein, however, are derived from a totally different and chemi-cally unrelated 6,6,6 tricyclic aromatic ring system which differs substantially from the fluorene nucleus. To applicants' knowledge the compounds described and claimed herein are novel compounds which have not been previously described nor reported in the literature. AdditionallyJ
applicants are unaware of any previously reported bis-basic derivatives of 9-phenanthrol, 6(5H)-phenanthridinone or 6H-dibenzo[b~d]pyran-6-one which possess antiviral 30 activity. The compounds described herein possess a wide : . , . . .. . , : . . .
,. . ~ ... . : :
:-: . , -Canada ~ 39 spectrum of antiviral activity in varying degrees which could not have been predicted from a knowledge of the present state of the art.
SUMMARY OF THE INVENTION
' ', ~
This invention relates to new derivatives of 9-sub-stituted phenanthrene and to their related 10-oxa and 10-aza congeners, to their methods of preparation, compositions ~ -~
thareof, and to their usefulness as pharmaceutical agents. : :
More particularly, the compounds of the present invention are 2,7-bis-basic ethers of 9-phenanthrol or 9-lower alkoxyphenanthrene, 3,8-bis-basic ethers of 6(5H)-phenan-thridinone and 3,8-bis-basic ethers of 6H-dibenzo[b,d~pyran-6-one which are useful in the prevention or inhibition of viral infections. Still more particularly, the com-pounds of the present in~/ention may be represented by ~
the following general formulas: ~.
R
,-R1 ~ o-(CH2)n-~N-(CH2)n-O
and R1`
~-R1 ~ O-(CH2)n-N \ R ' R ~N-(CH2)n O ~ Y
( I l ) -wherein n is an integer of from 2 to 6; R and Rl are each selected from the group consisting of hydrogen, lower alkyl ,~ 4 ''!~ _ M~677 Canada 3~
having from 1 to 6 carbon atoms, cycloalkyl having from 3 to 6 carbon atoms, alkenyl having from 3 to 6 carbon atoms in which the unsaturation is in a position other than the 1-position of the alkenyl group, and when R and R1 are taken toge~her with the nitrogen atom to which they are attached represent the pyrrolidinylJ piperidino or morpholino radical; Z is selected from the group consist-ing of oxygen, imino and methylene; Y is nitrogen or methylidene; and R2 is selected from the group consisting of hydrogen or lower alkyl having from 1 to 4 carbon atoms. The expression methylene is intended to refer to the -CH2- radical, whereas the expression methylidene is intended to refer to the -CH= radical.
The compounds represented in formulas (I) and (Il) above include both the free base form as well as the pharmaceutically acceptable acid addition salts thereof.
In generalJ the salts of these compounds are crystalline materials which are soluble in water and various hydro-philic organic solvents, and which, in comparison to their free base forms, generally exhibit higher melting points and an increased stability.
It should be noted that the bis-basic ether side chains appear in the same relative configuration for the 9-substituted phenanthrene, 6(5H)-phenanthridinone and the 6H-dibenzo[b,d]pyran-6-one series of compounds. However, due to the difference in numbering systems as shown below, the compounds of the present invention are designated as
; This invention relates to new organic chemical com- pounds, to their preparation and to pharmaceutical compo-sitions çontaining such compounds. The compounds de-scribed herein are useful antiviral agents which inhibit or inactivate viruses by their administration to either an infected qr to a non-infected host.
~-BACKGROUND OF THE INVENTION
There is a growing body of information that viruses play a vital role in a broad range of diseases, some of which represent the most serious of man's ills. Arthritis~
juvenile arthritis, diabetes, Hodgkin's disease and various immunological diseases and degenerative diseases of the central nervous system have been linked to viruses as the causative agents.
At present, the control of virus infecttons is primarily achieved by means of immunization vacc;nes.
' ~
- WJS:dw . 1-.
~ 39 For examp 1 e, po 1 i omye 1 i tisl sma 1 1 pox, meas 1 es and i nf 1 uenza are well recognized diseases in which viral vaccines have proven effective. In general, however, viral vaccines have had only a moderate success in animal prophylaxis.
Each vaccine acts primarily against a specific virus and is not heterophilic in the protection it offers. Hence~
vaccines do not provide a practical solution against the wide array of infectious viruses, even where limitedg as for example, solely to respiratory viruses.
One approach to the control of virus-related diseases and, particularly to the spread of such virus diseases, ;
has been to search for mediclnal agents or chemothera- -peutic agents which are capable of inhibiting the growth of viruses, thereby preventing the spread of disease as well as preventing further damage to cells and tissues ~`
of the animal host which have not as yet been infectedO ;
Heretoforeg only a limited number of virus infections such as smallpox, Asian influenza and herpes~keratitis have been susceptible to prevention by chemical antiviral agents. Sulfonamides and ant;biotics which hàve revo--lutionized the treatment of bacterial infections have substantially no effect upon virus infections. Certain infections caused by large v;ruses, such as iymphogranuloma venereum, psittacosis and trachoma have been successfully treated using antibiotics and sulfa drugs. However~ the majority of infections have not been respons;ve to attack by chemotherapeutic agents. Thus, it can be seen that there is a need for new chemotherapeutic agents which are effective against a broad range of virus diseases9 and which at the same time, are non-toxic to the;host.
: ., ~' ' - : ' - :
: .' . ' , , :
Canada ~ ~ Z ~ 39 As a result of a long series of investigations, applicants have discovered a novel class of antiviral agents which are bis-basic ether derivatives of 9-sub-stituted phenanthrene and which also include their corresponding 10-oxa and 10-aza derivatives. A more descriptive designation for these compounds in accord-ance with Chemical Abstracts nomenclature would be to describe them as bis-basic ether derivatives of 9-phenan-throl, 6(5H)-phenanthridinone and 6H-dibenzo[b,d]pyran-10 6-one. These compounds are effective against a wide spectrum of viral infections and are useful in treating such infections either prophylactically or therapeutically.
U.S. Patent 3,592,819 is the closest art known to applicants and discloses certain bis-basic ethers and thioethers of various substituted fluorenesJ 9-fluorenols and 9-fluorenones useful as antiviral agents. Certain of the bis-basic ethers described therein serve as starting materials for the preparation of some of the compounds of the present invention.
The bis-basic ethers described and claimed herein, however, are derived from a totally different and chemi-cally unrelated 6,6,6 tricyclic aromatic ring system which differs substantially from the fluorene nucleus. To applicants' knowledge the compounds described and claimed herein are novel compounds which have not been previously described nor reported in the literature. AdditionallyJ
applicants are unaware of any previously reported bis-basic derivatives of 9-phenanthrol, 6(5H)-phenanthridinone or 6H-dibenzo[b~d]pyran-6-one which possess antiviral 30 activity. The compounds described herein possess a wide : . , . . .. . , : . . .
,. . ~ ... . : :
:-: . , -Canada ~ 39 spectrum of antiviral activity in varying degrees which could not have been predicted from a knowledge of the present state of the art.
SUMMARY OF THE INVENTION
' ', ~
This invention relates to new derivatives of 9-sub-stituted phenanthrene and to their related 10-oxa and 10-aza congeners, to their methods of preparation, compositions ~ -~
thareof, and to their usefulness as pharmaceutical agents. : :
More particularly, the compounds of the present invention are 2,7-bis-basic ethers of 9-phenanthrol or 9-lower alkoxyphenanthrene, 3,8-bis-basic ethers of 6(5H)-phenan-thridinone and 3,8-bis-basic ethers of 6H-dibenzo[b,d~pyran-6-one which are useful in the prevention or inhibition of viral infections. Still more particularly, the com-pounds of the present in~/ention may be represented by ~
the following general formulas: ~.
R
,-R1 ~ o-(CH2)n-~N-(CH2)n-O
and R1`
~-R1 ~ O-(CH2)n-N \ R ' R ~N-(CH2)n O ~ Y
( I l ) -wherein n is an integer of from 2 to 6; R and Rl are each selected from the group consisting of hydrogen, lower alkyl ,~ 4 ''!~ _ M~677 Canada 3~
having from 1 to 6 carbon atoms, cycloalkyl having from 3 to 6 carbon atoms, alkenyl having from 3 to 6 carbon atoms in which the unsaturation is in a position other than the 1-position of the alkenyl group, and when R and R1 are taken toge~her with the nitrogen atom to which they are attached represent the pyrrolidinylJ piperidino or morpholino radical; Z is selected from the group consist-ing of oxygen, imino and methylene; Y is nitrogen or methylidene; and R2 is selected from the group consisting of hydrogen or lower alkyl having from 1 to 4 carbon atoms. The expression methylene is intended to refer to the -CH2- radical, whereas the expression methylidene is intended to refer to the -CH= radical.
The compounds represented in formulas (I) and (Il) above include both the free base form as well as the pharmaceutically acceptable acid addition salts thereof.
In generalJ the salts of these compounds are crystalline materials which are soluble in water and various hydro-philic organic solvents, and which, in comparison to their free base forms, generally exhibit higher melting points and an increased stability.
It should be noted that the bis-basic ether side chains appear in the same relative configuration for the 9-substituted phenanthrene, 6(5H)-phenanthridinone and the 6H-dibenzo[b,d]pyran-6-one series of compounds. However, due to the difference in numbering systems as shown below, the compounds of the present invention are designated as
2,7-bis-basic ethers for the phenanthrene series of com-pounds~ whereas they are designated as 3,8-bis-basic ethers in both the phenanthridine and 6H-dibenzo[b,d]pyran series -2~3~3 of compounds.
~:' ~3 ~33 Phenanthrene Phenanthridine 6H-Dibenzo[b,d]pyran The 2,7-bis-basic ethers of 9-methoxyphenanthrene (V) are prepared via a ring enlargement of the related 2,7-bis basic ethers of fluoren-9-one (Il 1)3 which are found described in U.S. Patent 3,592,819. This series of re-actions can best be illustrated by means of the following reaction scheme in which the symbols n, R~ R~ and R2 have the values previously assigned.
,-Rl O Rr \N-(cH2)n-o ~O-(CH2)n-N~
( I I 1) C H 2 N2-~
10 ,'Rl ~ O-( CH2)n-N ~ , ~ R ~N-(CH2)n-O ~ . :~
O ~ , ,-RI ~ O-(CH2) ` R /N-(CH2)n-O
OH
~IV) , . ; , . . . : . . . .
Canada R
(cH2)n-o~ $J o-(cHz)n-N\
._ . ......
R
¦ ( R /N~(CH~)n~ ~ O-(CH2)n-(V) , .
rR1\ ~ O-(CH2)n-N\R J
R ~N-(CH2)n-O ~ R2 t I I ) The 3,8-bis-basic ethers of 6(5H)-phenanthridinone ~VI) are prepared via a hydrazoic acid ring expansion using the 2,7-bis-basic ethers of fluoren-9-one (111) in the -presence of a strong mineral acid. This reaction can be :
illustrated by the following general reaction scheme in which the symbols n~ R and R1 have the same values pre- .
viously assigned:
.,.~
- ~ . . .
-, ~ .
:, . .
M- G ~7 ~4;~
, ' ~1 R l"
R ~N-(CH2)n-O~ (CHz)n-~.
( I I I ) NaN3 ~(CII: )n~N \
( V 1 ) The 3,8-bis-basic ethers of 6H-dibenzo[b,d]pyran-6- ~ :
one (X) can be prepared by means of a peractd or peroxide ring expansion of the related 2,7-bis(w-haloalkyl)ethers :
of fluoren-9-one (Vll) in the presence of a strong mineral acid. The 3,8-bis(w-haloalkyl)ethers of 6H-dibenzo[b,d]
pyran-6-one (Vlli) so prepared are subsequen~iy condensed with an amine (IX) to form the desired 3,8-bis-basic -ethers of 6H-dibenzo[b,d]pyran-6-one (X). Th;s reaction sequence can be illustrated by the following genera1 reaction scheme in which the symbols n, R and R1 have the values previously designated and the symbol Hal is chlorine, bromine or iodine.
~ _ .
.
~:' ~3 ~33 Phenanthrene Phenanthridine 6H-Dibenzo[b,d]pyran The 2,7-bis-basic ethers of 9-methoxyphenanthrene (V) are prepared via a ring enlargement of the related 2,7-bis basic ethers of fluoren-9-one (Il 1)3 which are found described in U.S. Patent 3,592,819. This series of re-actions can best be illustrated by means of the following reaction scheme in which the symbols n, R~ R~ and R2 have the values previously assigned.
,-Rl O Rr \N-(cH2)n-o ~O-(CH2)n-N~
( I I 1) C H 2 N2-~
10 ,'Rl ~ O-( CH2)n-N ~ , ~ R ~N-(CH2)n-O ~ . :~
O ~ , ,-RI ~ O-(CH2) ` R /N-(CH2)n-O
OH
~IV) , . ; , . . . : . . . .
Canada R
(cH2)n-o~ $J o-(cHz)n-N\
._ . ......
R
¦ ( R /N~(CH~)n~ ~ O-(CH2)n-(V) , .
rR1\ ~ O-(CH2)n-N\R J
R ~N-(CH2)n-O ~ R2 t I I ) The 3,8-bis-basic ethers of 6(5H)-phenanthridinone ~VI) are prepared via a hydrazoic acid ring expansion using the 2,7-bis-basic ethers of fluoren-9-one (111) in the -presence of a strong mineral acid. This reaction can be :
illustrated by the following general reaction scheme in which the symbols n~ R and R1 have the same values pre- .
viously assigned:
.,.~
- ~ . . .
-, ~ .
:, . .
M- G ~7 ~4;~
, ' ~1 R l"
R ~N-(CH2)n-O~ (CHz)n-~.
( I I I ) NaN3 ~(CII: )n~N \
( V 1 ) The 3,8-bis-basic ethers of 6H-dibenzo[b,d]pyran-6- ~ :
one (X) can be prepared by means of a peractd or peroxide ring expansion of the related 2,7-bis(w-haloalkyl)ethers :
of fluoren-9-one (Vll) in the presence of a strong mineral acid. The 3,8-bis(w-haloalkyl)ethers of 6H-dibenzo[b,d]
pyran-6-one (Vlli) so prepared are subsequen~iy condensed with an amine (IX) to form the desired 3,8-bis-basic -ethers of 6H-dibenzo[b,d]pyran-6-one (X). Th;s reaction sequence can be illustrated by the following genera1 reaction scheme in which the symbols n, R and R1 have the values previously designated and the symbol Hal is chlorine, bromine or iodine.
~ _ .
.
3~
O
~al-(CH2)n~ ~r -( CH2) n-Ha 1 + H22 ~---(Vll) Strong Acid ~ O- ( CH2 ) n-Ha 1 -\ ~N-H + Hal-(~H2) -O ~ ~~~~~ ;
(IX) (Vlll) R
L ~o-(cH8) ~ ~
(X, ~..... ~
To achieve an antiviral effect the compounds of this invention are administered to a suitable host using a variety of compositions. Such compos~tions-may be admin~
istered either prior to inf~ctiong as~a prophylactic use or treatment, or they may be administered subsequent to infection of the host as a curative use or tr~eatment.
A wide variety of compositions are also included with-in the scope of the present invention~ Thus, the instant compounds may be applied externally or topically directiy - at the situs of infection, or they may be administered internally or systemically, irrespective of whether the treatment is prophylactic or curative in na~ure. In either _g_ .. . . .................... . . .... :
.. ... ... ..
M~677 ~ ~ Z ~ 39 event, replication of the infectious virus is inhibited or preyented with the concomitant result that the variou~
disease symptoms characteristic of the pathogenic viral infection are either diminishecl or no longer present~
DETAILED DESCRIPTION OF THE INVENTION
As can be seen from general formulas (I) and (Il) above, the compounds of the present invention encompass bis-basic ethers in which each side chain is linked to a ~ ;
benzenoid portion of the tricyclic nucleus. More spe-cificallyg these side chains are linked at the 2 and 7_ positions in the phenanthrene series and at the corre-sponding ~ and 8-positions in the phenanthridine and 6H-dibenzo[b,d]pyran seriesO It can be further seen that the side chains consist essentially of a basic amino function at the terminal end of the chain, an ether bridging group at the proximal end of the chain with the basic amino function separated from the ether group by an alkylene chain of prescribed length. `~
The basic amino function represented by the symbol -N ~R `~ can be a primary, secondary or a tertiary amino group. Preferablyy each amino group ;s a tertiary amine The symbols R and R1 represent either hydrogen or a lower alkyl group. The term lower alkyl as used herein with regard to the basic amino function relates to groups having from 1 to 6 carbon atoms. Illustrative of such groups can be mentioned both straight or branched chain alkyl radicals such as: methyl~ ethyl, n-propyl, iso propyly n~butyl, sec-butyl, isoamyl, n-pentyl and ., ~ ~ -..
~ 24~39 n~hexyl. When R and R~ each represent lower alkyl, a preferred subgenus ;s formed.
Each R and Rl can also represent a cycloalkyl group having from 3 to 6 carbon atoms. Illustrative of such groups are the cyclopropyl~ cyclobutyl, cyclopentyl and cyclohexyl radicals.
The symbols R and R1 also represent an alkenyl group having from 3 to 6 carbon atomsO In addition to the un-saturation which must be present, this unsaturation must be in a position other than the 1-position of the alkenyl group in order to prevent hydrolysis from occurringO
lllustrative of such groups are the allyl~ 3-butenyl and '~
the 4-hexenyl radicals.
R and R~ may also be joined with the nitrogen atom to which they are attached to represent various saturated ~, monocyclic, heterocyclic radicals. Typical of such hetero~
cyclic groups are the 1-pyrrolidinyl9 piper;dino or '~
morpholino radicals~ Compounds containing these groups are,readily prepared and typify saturated monocycl iC9 heterocyclic radicals which are generally useful in lieu of the dilower alkylamino groups present in the compounds of this InventionO
The alkylene chain separating the basic amino function from the tricyclic ring consists of from 2 to 6 carbon atoms and can be either a straight or branched alkylene chain. the alkylene chain must separate the adjacent oxygen atom from the terminal amino nitrogen by at least 2 carbon ato~s9 i.e.9 the ether oxygen and amino nitrogen cannot share the same carbon atom of the alkylene group.
Each of the alkylene groups can be the same or dtfferent~
~ 4Z4~
preferably, however, both alkylene groups are the same.
Illustrative of such groups are ethylene, propylene, 1,3-propylene, butylene, 1,4-butylene, 2-methyl-1,4-but~
ene, pentamethylene, 3-methyl-1,5-pentylene`and hexameth~
ene.
Illustrative of the base compounds of the present invention represented by generic formula (I) can be men- `~
tioned: 398-bts(4-piperidinobutoxy)-6(5H)-phenanthridinone, 2,7-bis[2-(diethylamino)ethoxy]-9-phenanthrol, 3,8-bis~3~
(N-cyclohexyl-N-methylamino)propoxy]-6H-dibenzo[b,d~pyran-6-one, ~,8-bis[2-(diisopropylamino)ethoxy]-6(5H)-phenan-thridinone, 398-bis[2-(diisopentylamino)ethoxy]-6H-dibenzo [b,d]pyran-6-one and 2,7-bls[2-(diallylam;no)ethoxyJ-g-phenanthrolO '~
It must also be recognized that compounds of formula ~ ~
(i) in which a hydrogen atom Is avallable adjacent to the ~ ~ -keto function are capable of forming the corresponding enol tautomers. Thus, in both the phenanthrene and the ~
6H-phenanthridone seriesg the compounds of the present ;~-invention may also be represented by the general formula (IA) which is tautomeric with the corresponding keto Form as illustrated below.
.
, ,~
lL~4'~39 R l`, ~Rl ~J ~(CH2)n~N\ R ' , N- ( C 112 ) " - H
( I) O- ( C H2 ) n - N
R ,N-(CH2)n-O
OH
(IA) In the above representation, it is understood that the symbol Y is limited to nitrogen and the.CH. group only . Where the oxygen atom is present,as in the-6H-dibenzo[b,d]pyran series of compoundsg there is, of course, no~ enoi.îzable hydrogen available and the compounds exist only in their keto form. It is further understood that the~compounds in the phenanthrene and phenanthridine series are li.kely to be mixtures of tautomeric forms, the compositions of which are dependent upon such factors as the nature of the tri-cyclic nucleus, the various side cha;ns present and the environment surrounding the molecule as a whole. In the case of the phenanthrene series, the enol or phenolic form predominatesO
The enol form can be stabil;zed by a replacement of the enol hydrogen in this position with a lower alkyl group as represented by the symbol R2. Thus~ the 6 and/or 9-lower alkyl ethers exist only in compounds of the phenanthrene and phenanthridine series respectively, wherë-as the 6H-dibenzo[b,d]pyran series of compounds exist ~ ~ Z ~3~
only as 6-keto compounds and do not form the corresponding 6-lower alkyl ethers. Illustrative of the base compounds of the present invention represented by general formula (li) there can be mentioned: 2,7-bis[2-(diethylamino) ethoxy]-9-methoxyphenanthrene, 2,7-bis[3~ pyrrolidinyl) propoxy~-9-propoxyphenanthrene, 2,7-bis[2-(cyclohexylamino) ethoxy]-9-methoxyphenanthrene, 2,7-bis[4-(diallylamino) butoxy]-9-methoxyphenanthrene~ 3,8-bis[2-(diethylamino) ethoxy]-6-methoxyphenanthridine and 398-bis[4-(dimethyl-amino)butoxy]-6-ethoxyphenanthridine.
The expression "pharmaceutically acceptable acld addition salts" is Intended to apply ~o any non-toxic organic or inorganic acid addition salts of the base com-pounds represented by formulas (I) and (Il). Illustrative inorganic acids which form suitable salts include hydro-chloric, hydrobromic, sulfuric and phosphoric a~ids as ;
well as acid metal salts such as sodium monohydrogen orthophosphate and potassium hydrogen sulfate. Illustra-tive organic acids which form suitable salts include the mono, di and tricarboxylic acids. Illustrative of such acids are,ifor example, acetic, propionic, glycolic, lactic, pyruvic9 malonic, succinic, glutaricg fumaricg malicg tartaric, citric~ ascorbicg maleic, hydroxymaleic benzoic, p-hydroxybenzoicg phenylacetic, cinnamic, salicylic, 2-phenoxybenzoic and sulfonic acids such as methanesulfonic acid and 2-hydroxyethanesulfonic acid~
Either the mono or the di-acid salts can be formed, and such salts can exist in either a hydrated or a sub stantially anhydrous form.
In general the compounds of the present Tnvention , . , - . . ,, . :
.. . - . .. , , ... . . . . . ~,.
, : . . . .
.. : . . . :...... ~ , .. .
3LV4~39 are prepared From various 2,7-disubstituted-9-fluorenone precursors which are more fully described in U.S. Paten~
3,592,~19. The preparation of the instant compounds involv~s various ring expansions of the g-fluorenone nucleus to form the corresponding g-substituted phenan-threne, 6(5H) phenanthridlnone and 6H-dibenzo[b,d]pyran-6-one rings. More particularly, the reaction with diazo-methane results in the formation of the 2,7-bis bas;c ethers of 9-methoxyphenanthrene; the reaction with hydrazoic acid results in the formation of 3,8-bis basic ethers of 6(5H)-phenanthridinone; whereas the reaction with peracids or hydrogen peroxide results in the formation of the 3,8-bis basic ethers of 6H-dibenzo[bgd]pyran 6-one.
Cyclic aliphatic ketones are known to ring expand with diazomethane-to form larger cyclic aliphatic ketonesO
This reaction may also be conducted in some instances with aromatic ketones, producing a variety of homologous ring-enlarged ketones in addi~ion to the formation of enols, enol ethers and ethylene oxidesO Thusg for example, Schultz et alg J. Am. Chem SocO 62g 2902-4 (1940) reported the reaction of diazomethane with fluorenone to yield 5% of 9-phenanthrol, 30% of 9-methoxyphenanthreneg 1.5~ of di-9-phenanthryl etherg an unknown substance and 30~ of unchanged fluorenoneO In additiong substituents on the aromatic ring are known to change the ratio of the various products obtained as well as to give rise to additional isomericJ homologous ketones and side productsO ;
Thus, the application of the diazomethane reaction to the 2,7-bis-basic ethers of 9-fluorenone could not have been predicted and is not without difficultyO
Canada ~ ~f~ 39 The reaction is most frequently conducted by treat-ment of a methanolic solution of the carbonyl containing compound with an ethereal solution of diazomethane, either in the presence or absence of a catalyst. Alternatively, a solution of the carbonyl compound in methanol is treated with nitrosomethylurethane in the presence of a base. The diazomethane can be prepared either ex situ or in situ. Generally, applicants prefer to generate the diazomethane ex situ and to co-distill t-he diazomethane so produced with ether into a methanolic solution of the carbonyl containing compound. Suitable inert solvents which may also be utilized include such solvents as dioxane, benzene, toluene, chloroform and methylene chloride with ether-methanol being the solvent combination of choice. Additionally, methanol has been shown to have a high catalytic activity for dia~omethane ring expansion reactions. Catalysts which may be usefully employed in this reaction include trace amounts of metal salts such ;~
as zinc chloride or lithium chloride. A minimum of 2 equivalents of diazomethane are generally employed, one equivalent providing for the ring expansion, whereas the other equivalent competes with the starting material to form the corresponding 9-methoxyphenanthrene ethers (V).
In addition to a variety of side products which are formed, some of the intermediate 2,7-bis basic ethers of 9-phenanthrol (IV) also remain. The intermediates are readily separated from the reaction mixture in the form of their sodium salts. The 9-lower alkoxyphenanthrene derivatives (II), other than the 9-methoxyphenanthrene ~o derivatives, are prepared, in turn, by the reaction of the B
~ 4 ~ ~ 3~
9-phenanthrols (IV) with other lower diazoalkanes.
Due to the complex and wide variety of side reactions possible, no more than 8 equivalents of diazomethane are' useful in the preparation of the 2,7-bis-basic ethers g-methoxyphenanthreneO The reaction proceeds exothermall~
and is conducted at room temperature or below. The re-action takes place at a temperature range of from about -50C. to ambient temperatur-es with a temperature of 0C.
conveniently preferred. The reaction can be conducted for a period ranging anywhere from about,one hour to about seven days.
- The 9-phenanthrol derivatives~ which are also formed in the diazomethane ring expansion reaction,are readily freed of their nonacidic materials by extraction with chloroform from a strongly alkaline reactlon mixture.
The 9-phenanthrol derivatives which remain in the aqueous medium may then be removed by neutralization of the aqueous medium to a pH of about 9-10, and subsequently extracting the neutralized rnedium with chloroformO
The 9-phenanthrols so obtained can then be etherified by reacting with a lower diazoalkane to form the corresponding 9-lower alkoxy phenanthrene ethers~
The preparation of the 398-bis-baslc ethers of 6(5H) phenanthridinone (VI) is achieved via a modification of the so-called Schmidt reaction. Essentially, as applied here, this method inserts a hetero nitrogen atom via a ring expansion into the five membered fluoren-9-one ring using hydrazoic acid in the presence of a strong mineral acid such as sulfuric acid to form the six-membered ~0 heterocyclic ring of 6~5H)-phenanthridone. Hydra20ic ' ' . ' ' ! . ' ~ ' ' ' . ' ' ' . ' : ' acid is ~nown to react with simple cyclic ketones to form ring enlarged cyclic amides or lactams. Thus, for example, cyclohexanone reacts with hydrazoic acid yielding caprolactam. However, to applicants' knowledge, the use of hydrazoic acid on a 9-fluorenone nucleus containing a bis-basic ether side chain has not previously been reported. Approximately equimolecular quantities of hydrazoic acid are used inasmuch as an excess of hydrazoic acid encourages tetrazole formation. The reaction can be conducted by the addition of a solution of hydrazoic acid in an appropriate organic solvent to a solution of the fluoren-9-one. Alternatively, hydrazoic acid can be generated ln situ by the addition of sodium azide to the reaction mixture. Due to the extremely hazardous nature of hydrazoic acid, the use of sodium azide is preferred, thereby eliminating the necessity for the isolation of the toxic hydrazoic acid. This reaction is exothermic in nature, and consequently the reaction is best conducted by stirring with a suitable means for cooling. The reaction can be conducted at temperature ranges of from about -20C. to about 50C., and for periods of from about 30 minutes to about one week. Generally, a temperature of 0C. with a reaction period of about 1 hour is preferred as a matter of convenience. If the reaction appears to proceed slug-gishly, higher temperatures can be advantageously employed. Due to the rapidity of the reaction with hydrazoic acid, the reaction is conveniently controlled by the rate of addition of the hydrazoic acid or sodium azide to a stirred solution of the 9-fluorenone.
~' ' .
~ -, ~ ,. .' ' ' . . - , . . .
derivative dissolved or suspended in a suitable solvent.
Suitable solvents include sulfuric acid, chloroform, benzen~ dioxane and diethyl ether with trifluoroacetic acid h~ving been found to be particularly useful.
Generally, sodium azide is added in small increments to a trifluoroace~ic acid solution of the g-fluorenone ether derivat;ve, until no further evolution of nitrogen gas is observed. At this point the reaction is con-sidered to be complete for all practical purposes. An acid catalyst is also employed. Any strong acid may be used with concentrated sulfuric acid being the catalyst of choice. Isolation of the 3,8-bis basic ethers of 6(5H)-phenanthridinone so prepared is achieved using standard procedures apparent to those skilled in the art.
An alternative route to the preparation of the 6(5H)-phenanthridinone ethers is via a Beckmann rearrangement of the corresponding fluorenone oximes. Moore and Huntress, J. Am. Chem. Sc. 49, 2618 (1927), have demon~
strated the preparation of 7-nitro-6(5H)-phenanthridinone by the treatment of 2-n;trofluoren-9-one oxime with phosphorous pentachloride dissolved in phosphorous oxy- -chloride. In similar fashion the 3,8-bis basic ethers of 6(5H)-phenanthridinone can be prepared from the 2,7-bis-basic ethers of fluoren-9-one oxime as illustrated in the following reaction scheme:
- 19- ~
Z~39 N - ~ CHz ~ n - ~ O - ( C H2 ) - N /
POCl3 PCls NC1 Rl``
~N-(CH2)n-O~JO-(cH2)n-N~ ~ Ç .
Heat ~ . /R
- L ~R~ (CH2) -(CH2)n-O ~Cl Rl~
,~R 1 o~ 0~( CH2) n~N \ R ~ ¦
\N-(CH2)n-~R ~ OH
1~ ..
, R ~ ~ O- ( C H8 ~ ~ - N
,N_~HZ)n-o H
R O
M-~77 ~)4Z439 The first step in the preparation of the 3,8-bis-basic ethers o~ 6H-dibenzo[b,d~pyran-6-one involves the peracid or peroxide oxidation of a 2~7-bis(w~haloalkyl) ether of fluoren-9-one (Vll) under Baeyer-Villiger conditions. Essential 1YJ this reaction results ;n a ring expansion of the five-membered fluoren-9-one ring with the introduct10n of an oxygen atom to form a six-membered ring expanded lactone or 6H-dibenzo[b,d]
pyran-6-one nucleus. Thus, the oxidation of a 2,7-bis (w-haloalkyl~ ether of fluoren-g-one (Vll), found described in U.S. Patent ~5g2g819~ with a peracid or hydrogen peroxide in the presence of a strong acid~ such as sulfuric acid, results in the formation of a ~,8-bis (w-haloalkyl) ether of 6H-dibenzo[b,d]pyran-6-one (Vlll).
The 6H-dibenzo[bld]pyran (w-haloalkyl) ethe~s so pre-pared can then be condensed with an amine (IX) to form the desired 3,8-bis-basic ethers of 6H-dibenzo[bJdJpyran-6-one (X), the compounds of the present inventionO The -oxidation reaction is exothermic in nature and the temp-erature of the reaction mixture may be conveniently controlled by the rate of additi~n of the oxidizing agent.
The reaction temperatures ~ary from about -20 to 40C., with a temperature of about 25~C. being preferred~ ~ -Reaction times range anywhere from about ~0 minutes to about one week at the lower temperatures. Large excesses of peroxide are to be avoided inasmuch as there is always the hazard o-F peroxide oxidations occurring with explosive violenc;e. IF significant amounts of peroxide remain upon completion of the reaction, they can be decomposed using reducing agents such as sodium bisulfite or ferroùs ..
.
M-67rl~
1~4Z~39 sulfate. Generally, a two or three fold excess of hydrogen peroxide is satisfactory in carrying out the ring expansion.
The reaction is favored by polar solvents and proceeds in a variety of solvents such as sulfuric acid, acetic acid or acetic anhydride. The 3,8-bis(w-haloalkyl) ethers of 6H-dibenzo[b,d]pyran-6-one (Vlll) so prepared are sub-sequently condensed for the second step in the preparation of the 3,8-bis basic ethers of 6H-dibenzo[b,d]pyran-6-one ( X ) ~
The condensation reaction of the bis(~-haloalkyl) ethers (Vlli) with an amine.(IX) can be carrTed out using a variety of conditions. For example, the ethers can be heated together with a large excess of amine, the excess amine serving both as the reaction medium and the hydro-halide acceptorO This method is particularly suitàble for those amines which are readily available9 inasmuch.
as any excess amine can be readily removed from the ;~
reaction mixture via distillation at a reduced pressure or by steam distillation. Alternatively,.the w-haloalkyl ethers (Vlll) can be heated with an excess of~the amine in a suitable organic solvent. Suitable organic sol-vents include benzene, toluene, xylene and chlorobenzene, lower molecular weight alcohols such as methanolg ethanol -and isopropyl alcoholg or they may include .lower molec-ular weight ketones such as acetone and methyl ethyl ketone. The reaction of these ethers with an amine is generally promoted by the addition of either~ sodium or potassium iodide, the iodide being used in either cata-lytic or stoichiometric amounts. In some casesJ as when the amine is expensive or in short 5Upp 1 y, it may be .
:
~042~L39 advantageous to use only two equivalents of the amine for each equivalent of the ~-haloalkyl ether employed in the presence of an excess of e i ther powdered sodium or potassium carbonate as the acceptor for the hydrohalidç
that is ~enerated. In the case of volatile amines the condensation reaction can best be conducted under pressure in a suitable bomb or autoclave.
The compounds of the present invention are antiviral agents. Preferably they are administered to an animal host to prevent or inhibit viral infections. The term host refers to any viable biological material or intact animal including humans which is capable of inducing the formation of interferon and which serves as a support means for virus replicationO The host can be of animal ~ i or mammalian origin. Illustratively such hosts include birds, mice, rats, guinea pigs, gerb;ls, ferrets, dogs, cats, cows, horses and humans. Other viable biological mater;al such as used in the production of vaccines may also act as a host. Thus, tissue cultures prepared from organ tissues, such as mammalian kidney or lung tTssue9 as well as tissue cultures prepared from embryo tissue, such as obtained from amniotic cells or chick allantoic fluid, have been found to be useful hosts~
The treatment of virus infections for purposes of the present invention encompasses both the prevention and the inhibition of characteristic disease symptoms in a mammalian host susceptible to invasion by a pathogenic virus. Illustrative of mammalian virus infections which can be prevented or inhibited by the administration of ~0 the compounds of the present invention are infections -~3-- . .:: :. . . ~ .
M- ~77 ~ 0~43~
caused by picornaviruses, such as encephalomyocarditis virus; myxoviruses, such as influenza A2 (Jap/305) virus~
arboviruses; such as Semliki forest virus; the herpes group of viruses, including herpes simplex; and the po~-viruses, as for example vaccir~ia IHDo Thus9 for example~
the compounds of the present invent~on when administered orally or subcutaneously to mice in varying doses either shortly prior or subsequent to a fatal inoculation of a neurotropic virus such as encephalomyocarditis virus, having a LD50 anywhere from 5 to 50J delay or prevent completely the onset of death. Salts of these compounds are generally administered in compositions containing a 0.15% aqueous hydroxyethylcellulose vehicle, whereas the free base compounds are generally administered in compositions containing a 10~ aqueous surfactant vehicle in order to help solubilize the compound. In general~
ten mice are used for each treated group with an additional 20 mice serving as a control group. At the time of administration the test virus is titrated in order to determine the potency or LD50 for the particular virus pool used as a challenge. The control anima`ls are given a placebo containing the ident;cal volume of vehicle without, of course, the active ingredient. Because of the lethal ~ature of the test system employed, the anti-viral nature of the test compound is dramatically illustrated by a side by side comparison of the survival time of treated animals with the untreated control group of animalsO
Respiratory viruses, such as influenza ~2 ( Jap/305) ~0 virusJ which are also lethal to the test animals employed, ~ 4 Z ~ 3~
are administered via intranasal instillation. Animals infected in this manner have the active ingredients administered in the same manner as the test virus, and again a side by side comparison is made of the survivors of the animals ~reated with the untreated control animals.
Inexplicably, a mouse fcltally infected with encephalo-myocarditis or influenza virus occasionally survives with-out further treatment. This may be the result of a prior, interferon induced infection in the mouse9 or perhaps ~
due to some genetic factor or other natural defense mech- ' anism not presently understood. For this reason the control group selected is of sufficient size as to sta-tistically reduce to a negligible amount the influence of such a chancé survivor upon the test results.
The vaccinia test virus is typical of the dermato- ' ' trophic type viruses which respond to treatment with -compositions containing the compounds of the'instan~ `
Invention. The vaccinia virus generally produces a non-fatal infection in mice, producing characteristic tail lesions when the virus is subcutaneously'admi'nistered to the tail of the mouse. The instant compounds are adrninistered either orally or'subcutaneous-ly either prior '' ~;~''`
to or subsequent to the vaccinia infectionO Tail lesions are subjectively scored on the eighth day following infection against untreated animals which serve as a control group, The compounds of the present invention have been found to be effective in varying degrees against one or all of these test virus systems. ~ -The mode of activity of the active ingredients of the present invention is not rigorously defined. Inter .- . . . . .,~. . - ; .
; ~ ~ - . . , ~ .. , alia, the compounds ~ ~he present invention may induce the formation of interferon in a viable host. Interferon is a biological substance of unknown chemical structure, presumably proteinaceous in nature, which is produced by host cells in response to a viral infection. The inter feron so produced acts to induce a virus inhibiting su~
stance, which inhibits in some yet unknown manner the intracellular replication of the virus without appear-ing to hav`e any inactivation effect per se upon the virus itself. A few of the viruses susceptible to interferon replication inhibition are described in Horsfall and Tamm, "Viral and Rickettsial Infections of Man'~ 4th Edition (1965), J.B. Lippincott Company, pp. 328-9.
As previously indicated~ the compounds of the present invention may be prophylactically administered in order to prevent the spread of contagious viral diseases or they may be therapeutically administered to a host already infected intended for their curative effect. When admin-istered prophylactically3 it is pre~erred that the admin-istration be made within 0 to 96 hours prior to theinfection oF the host animal with a pathogenic virusi When the compounds of the present invention are admin-istered for their curative effect, it is preferred that they are admin'istered within about 1 or 2 days following infection of the host in order to obtain the maximum therapeutic effect.
The dosage to be administered will be dependent upon such parameters as the particular virus for which either treatment or prophyiaxis is desired, the species of animai ~0 involved, its age, health, weight, the extent of infection, ;. ' '' ' ~'~
Canada 1~?4i~439 concurrent treatmentg if ~ny~ frequency of treatment and the nature of the effect desired. A daily dose of the active ingredients will generally range from about 0.1 mg to about 500 mg per kg of body weight.. Illustratively dosage levels of the administered active ingredients for intravenous treatment range from about 0.1 mg to about 10 mg per kg of body weight; for intraperitoneal admin-istration range from about 0.1 mg to about 50 mg per kg of body weight; for subcutaneous administration range from about 0.1 my to about 250 mg per kg of body weight; for vral administration may be from about 0.1 mg to about 500 ~:
mg per kg of body weight; for intranasal instillation range .
from about 0.1 mg to about 10 mg per kg of body weight; an~
for aerosol inhalatian therapy, the range is generally from about 0.1 mg to about 10 mg per kg of body weight.
The novel compounds described herein can also be administered in various differPnt dosage unit forms, e.g., oral compositions such as tablets, capsules, dragees, :
lozenges, elixirs, emulsions, clear liquid solutions and suspensions; parenteral compositions such as intra-muscutar3 intravenous or intradermal preparations; and topical compositions, such as lotions, creams or ointments.
The amount of active ingredient contained in each dosage unit form will, o~ course, vary widely according to the ~.
particular dosage unit employed, the animal host being treated, and the nature of the treatment, i.e., whether prophylac~ic or therapeutic in nature. Thus, a particular dosage unlt may contain ~rom about 2.0 mg to over 3.0 9 of active ingredient in addition to the pharmaceutical excipients contained therein.
.. . .. . . . . . . .
.
. . .
~0~2439 The novel compounds described herein can be employed in conjunction or admixture with additional organic or inorganic pharmaceutical excipients. Suita'ble solid ex~ipients include gelatin, lactose, starches, magnesiu~
stearate and petrolatum. Suitable liquid excipients ~
include wa~er and alcohols such as ethanol, benzyl alcohol and the polyethylene alcohols either with or without the addition of a surfactantO In general~ the preferred liquid excipients particularly for injectable prep-arations, include water, saline solution, dextrose andglycol solutions such as an aqueous propylene glycol or an aqueous solution of polyethylene glycolO Liquid prep~. ' -arations to be used as sterile injectable'solutions will ordinarily contain from about 0.5% to about 25~
by weight, and preferably from about 1% t.o about 10~ .
by weight, of the active ingredient in solutionO In certain topical and parenteral preparationsl various ' oils are u~ilized as carriers or excipients.. Illustrative of such oils are mineral oils~ glyceride i!s. such as 20 ' lard oil, cod liver oil, peanut oil, sesame oil, corn oil and soybean oil. . . '.
-A suitable method of administrat'ion for the compounds of the present invention is orally either in:a soiid dose form such as a tablet or capsule, or in a liquid dose form such as an elixir, suspension, emulsion or.syrup. Ordi-narily the active ingredient comprises from about 0.5%
to about 10~ by weight of an oral liquid compositionO
In such compositions, the pharmaceutical carrier is generally aqueous in natureJ as for example,:aromatic ':
water, a sugar-based syrup or a pharmaceutical'mucilage.
,.. ,:~: - :
~ 342439 for insoluble compounds suspending agents may be added as well as agents to control viscosity, as for example, magnesium aluminum silicate or carboxymethylcellulose.
Buffers, preservatives9 emulsifying agents and other excipients can also be added.
For parenteral administration such as intramuscular intravenous or subcutaneous administration, the proportion of active ingredient ranges from about 0.05% to about 20%
by weight, and preferably from about 0.1~ to about 10%
by weight of the liquid COmpOSitiQn. In order to minimize or eliminate irritation at the site of injection, such compositions may contain a non-lonic surfactant having a hydrophile-lipophile balance (HLB) of from about 12 to about 17. The quantity of sùrfactant in such formu~
lations ranges from about 5% to about 15~ by weight. The -surfactant can be a single component having the above idèntified HLB, or a mixture of two or more components having the desired HLB. Illustrative of surfactants useful in parenteral formulations are the class of polyoxyethylene sorbitan fatty acid ethers as, for example, sorbitan monooleate and the high molecular weight adducts of ethylene oxide with a hydrophobic base, formed by the condensation of propylene oxide with propylene glycol.
The concentration of active ingredient contained in these various parenteral dosage unit forms varies over a broad range and comprises anywhere from about 0.05% to about 20% by weight of the total formulation, the remaining component or components comprising liquid pharmaceutical excipients previously mentioned.
The active ingredients of the present invéntion can , :-.,- . .
1t~4~39 also be admixed directly with animal feeds or incorpo-rated into the drinking water of animals. For most purposes, an amount of active ingredient is used which provides from about 0.0001~ to about 0.1% and preferably from abou-t 0.001% to about 0.02~ by weight of the active ingredient based upon the total weight o~ feed intake~
The active ingredients can be admixed in anima1 feed concentrates, suitable for use by farmers or livestock growers for incorporation in appropriate amounts with the final animal feeds~ These concentrates ordinarily .
comprise ~rom about 0.5% to about 95% by weight of the active ingredient compounded with a finely divided solid carrier or flour, such as wheat~ corn9 soybean or cottonseed flour. Depending upon the particular animal to be fed~ nutrients and fillers may also be added such as ground cereal, charcoal~ fuller's earth, oyster shells and finely divided attapulgite or bentoniteO ..
The active ingredients of the present invention can be packaged in a suitable pressurized container together ~ .
with an aqueous or volatile propellant for use as an aerosol. A suitable discharge valve is fitted to an. -opening in the container from which the active ingredi-ents may be conveniently dispensed in the form of a spray, liquid, ointment or foamO Additional adjuvants such as co-solvents, wetting agents and bactericides may be employed as necessary~ Normally, the propellant used is a liquified gaseous compound, preferably a mixture oF low molecular weight fluorinated hydrocarbons. These halo-alkanes are preferred bacause of their compatibility with the active ingredients of the present invention, and be~
~ V4Z~39 cause they are non-irritating when applied to skin surfac~s.
Other useful propellants include ethylene oxide, carbon dioxide, propane and nitrogen gas.
The invention described herein is more particularly illustrated by means of the following specific examples:
EXAMPLE I
.
2,7-Bis[2 (diethylamino)ethoxy]fluoren-9-one dihydrochloride A solution of [2-(diethylamino)ethyl]chloride obtained from 1505 9 (0.09 mole) of ~2-(diethylamino)ethyl]chloride hydrochloride in 100 ml of toluene (dried over molecular sieves) is added to a mixture of 6.4 g (0.03 mole) of 2J7-dihydroxy-fluoren-9-one and 3.3 g (o.o6 mole) of sodium methoxide in 200 ml of toluene (dried over molecular sieves). The resulting mixture is heated to its reflux temperature with stirrTng for a period of 3 hours. Upon cooling, the mixture is filtered to remove the precipi tated sodium chloride~ The toluene solution is washed 3 times with water, once with saturated sodium chlorid~
solution and dried over anhydrous magnesium sulfate~
This mixture is fiitered and the filtrate acidified to Congo Red with ethereal hydrogen chloride. The solid which precipitates is filtered, recrystallized from butanone with sufficient methanol being added to effect solution~ and the product dried at 100C. for 2~ hours under vacuum: m.p. 235-7C., ~ma 269~ and E 1~m 16000 EXAMPLE 1l ?,7-Bis~2-(diethylaminolethoxyl-9-methoxyphenanthrene A solution of 11.7 9 (0.028 mole) of 2,7-bis[2-(diethylamino)ethoxy]fluoren-g-one in 20 ml of ether and 50 ml of methanol is stirred and treated with approxi-' mately o.o8s mole of diazomethane which is co-distilled into the reaction mixture at 10-28C. with diethyl ether. The reaction mixture is stirred at room tempera-ture for 24 hours and most of the volatile materials are removed in vacuo. The residue is treated with a dilute solution of sodium hydroxide and extracted'several times with ether. The combined ether extracts are washed with water, dried over anhydroùs sodium~-sulfate, treate'd ' with charcoal, filtered and the product converted to -its hydrochloride salt by treatment with ethereal HCl.
This salt is then dissolved in 200 ml of ethanol and treated with 3.8 9 of G;rards Reagent T to allow for the subsequent removal of any unreacted starting material.
The resulting solution is refluxed for 3 hours, treated with 203 ml of a 5% sodium hydroxide solution and extracted with etherO The combined ether extracts are washed with water, dried and most of the volatile material removed. The resulting residue is~dissolved -~
in 40-60C~ petroleum ether, filtered, placed on an alumina'chromatographic column and eluted with 40-60Co petroleum ether. The initial eluate is collected, the volatiles removed and the 2,7-bis[2-(diethylamino)ethoxy]-9-methoxyphenanthrene so obtained is recrystallized from 40-60C~ petroleum ether and again from pentane solution, - -32- ' .
~042~39 ~
m.p. 63-5C., ~mae~H 259, and E 1~ 1590. -Following essentially the same procedure, but sub-stituting for the 2,7-bis[2-(diethylamino)ethoxy~
fluoren-g-one above) the appropriate molar equivalent quantities of 2J7-bis[2-(dibutylamino)ethoxy]fluoren-9~
one, 2~7-bis[2-(diisopropylamino)ethoxy]fluoren-9-one~ -2,7-bis[3-(dimethylamino)propoxy]fluoren-9-one and 2,7-bis[3-(dibutylamino)propoxy]fluoren-9-one, the following compounds are obtained: 2,7-bis[2-(dibutyl-amino)ethoxy]-g-methoxyphenanthrene, 2~7-bisC2-(diisopropyl-amino)ethoxy]-9-methoxyphenanthrene, 2,7-bisC~-(dimethyl-amino)propoxy]-g-methoxyphenanthrene and 2,7-bis[3-(dibutylamino)propoxy]-g-methoxyphenanthrene3 respectlvely.
' :
EXAMPLE lll ?,7-Bis(~piperidinopropox~) fluoren-9-one dihYdrochlorlde A mixture of 63.6 9 (0.30 mole) of 2J7-dihydroxy-~luoren-9-one, 188 9 (0.95 mole) of 1-(3-chloropropyl) piperidine hydrochloride, 132 9 (2.0 mole) of 85%
potassium hydroxide in 900 ml of toluene and 300 ml of water is refluxed with vigorous stirring for 20 hoursO
The layers are separated upon cooling and the organic layer is washed 3 times with water, once with a saturated solution of sodium chloride and dried over anhydrous magnesium sulfate. The mixture is filtered and the solvent removed tn vacuo. The residue is taken up in isopropyl alcohol and acidified to Congo Red with ethereal hydrogen chloride. The solid which precip;tates ~3-~ - - - , . i . .. . .. . . .. . .
Canada
O
~al-(CH2)n~ ~r -( CH2) n-Ha 1 + H22 ~---(Vll) Strong Acid ~ O- ( CH2 ) n-Ha 1 -\ ~N-H + Hal-(~H2) -O ~ ~~~~~ ;
(IX) (Vlll) R
L ~o-(cH8) ~ ~
(X, ~..... ~
To achieve an antiviral effect the compounds of this invention are administered to a suitable host using a variety of compositions. Such compos~tions-may be admin~
istered either prior to inf~ctiong as~a prophylactic use or treatment, or they may be administered subsequent to infection of the host as a curative use or tr~eatment.
A wide variety of compositions are also included with-in the scope of the present invention~ Thus, the instant compounds may be applied externally or topically directiy - at the situs of infection, or they may be administered internally or systemically, irrespective of whether the treatment is prophylactic or curative in na~ure. In either _g_ .. . . .................... . . .... :
.. ... ... ..
M~677 ~ ~ Z ~ 39 event, replication of the infectious virus is inhibited or preyented with the concomitant result that the variou~
disease symptoms characteristic of the pathogenic viral infection are either diminishecl or no longer present~
DETAILED DESCRIPTION OF THE INVENTION
As can be seen from general formulas (I) and (Il) above, the compounds of the present invention encompass bis-basic ethers in which each side chain is linked to a ~ ;
benzenoid portion of the tricyclic nucleus. More spe-cificallyg these side chains are linked at the 2 and 7_ positions in the phenanthrene series and at the corre-sponding ~ and 8-positions in the phenanthridine and 6H-dibenzo[b,d]pyran seriesO It can be further seen that the side chains consist essentially of a basic amino function at the terminal end of the chain, an ether bridging group at the proximal end of the chain with the basic amino function separated from the ether group by an alkylene chain of prescribed length. `~
The basic amino function represented by the symbol -N ~R `~ can be a primary, secondary or a tertiary amino group. Preferablyy each amino group ;s a tertiary amine The symbols R and R1 represent either hydrogen or a lower alkyl group. The term lower alkyl as used herein with regard to the basic amino function relates to groups having from 1 to 6 carbon atoms. Illustrative of such groups can be mentioned both straight or branched chain alkyl radicals such as: methyl~ ethyl, n-propyl, iso propyly n~butyl, sec-butyl, isoamyl, n-pentyl and ., ~ ~ -..
~ 24~39 n~hexyl. When R and R~ each represent lower alkyl, a preferred subgenus ;s formed.
Each R and Rl can also represent a cycloalkyl group having from 3 to 6 carbon atoms. Illustrative of such groups are the cyclopropyl~ cyclobutyl, cyclopentyl and cyclohexyl radicals.
The symbols R and R1 also represent an alkenyl group having from 3 to 6 carbon atomsO In addition to the un-saturation which must be present, this unsaturation must be in a position other than the 1-position of the alkenyl group in order to prevent hydrolysis from occurringO
lllustrative of such groups are the allyl~ 3-butenyl and '~
the 4-hexenyl radicals.
R and R~ may also be joined with the nitrogen atom to which they are attached to represent various saturated ~, monocyclic, heterocyclic radicals. Typical of such hetero~
cyclic groups are the 1-pyrrolidinyl9 piper;dino or '~
morpholino radicals~ Compounds containing these groups are,readily prepared and typify saturated monocycl iC9 heterocyclic radicals which are generally useful in lieu of the dilower alkylamino groups present in the compounds of this InventionO
The alkylene chain separating the basic amino function from the tricyclic ring consists of from 2 to 6 carbon atoms and can be either a straight or branched alkylene chain. the alkylene chain must separate the adjacent oxygen atom from the terminal amino nitrogen by at least 2 carbon ato~s9 i.e.9 the ether oxygen and amino nitrogen cannot share the same carbon atom of the alkylene group.
Each of the alkylene groups can be the same or dtfferent~
~ 4Z4~
preferably, however, both alkylene groups are the same.
Illustrative of such groups are ethylene, propylene, 1,3-propylene, butylene, 1,4-butylene, 2-methyl-1,4-but~
ene, pentamethylene, 3-methyl-1,5-pentylene`and hexameth~
ene.
Illustrative of the base compounds of the present invention represented by generic formula (I) can be men- `~
tioned: 398-bts(4-piperidinobutoxy)-6(5H)-phenanthridinone, 2,7-bis[2-(diethylamino)ethoxy]-9-phenanthrol, 3,8-bis~3~
(N-cyclohexyl-N-methylamino)propoxy]-6H-dibenzo[b,d~pyran-6-one, ~,8-bis[2-(diisopropylamino)ethoxy]-6(5H)-phenan-thridinone, 398-bis[2-(diisopentylamino)ethoxy]-6H-dibenzo [b,d]pyran-6-one and 2,7-bls[2-(diallylam;no)ethoxyJ-g-phenanthrolO '~
It must also be recognized that compounds of formula ~ ~
(i) in which a hydrogen atom Is avallable adjacent to the ~ ~ -keto function are capable of forming the corresponding enol tautomers. Thus, in both the phenanthrene and the ~
6H-phenanthridone seriesg the compounds of the present ;~-invention may also be represented by the general formula (IA) which is tautomeric with the corresponding keto Form as illustrated below.
.
, ,~
lL~4'~39 R l`, ~Rl ~J ~(CH2)n~N\ R ' , N- ( C 112 ) " - H
( I) O- ( C H2 ) n - N
R ,N-(CH2)n-O
OH
(IA) In the above representation, it is understood that the symbol Y is limited to nitrogen and the.CH. group only . Where the oxygen atom is present,as in the-6H-dibenzo[b,d]pyran series of compoundsg there is, of course, no~ enoi.îzable hydrogen available and the compounds exist only in their keto form. It is further understood that the~compounds in the phenanthrene and phenanthridine series are li.kely to be mixtures of tautomeric forms, the compositions of which are dependent upon such factors as the nature of the tri-cyclic nucleus, the various side cha;ns present and the environment surrounding the molecule as a whole. In the case of the phenanthrene series, the enol or phenolic form predominatesO
The enol form can be stabil;zed by a replacement of the enol hydrogen in this position with a lower alkyl group as represented by the symbol R2. Thus~ the 6 and/or 9-lower alkyl ethers exist only in compounds of the phenanthrene and phenanthridine series respectively, wherë-as the 6H-dibenzo[b,d]pyran series of compounds exist ~ ~ Z ~3~
only as 6-keto compounds and do not form the corresponding 6-lower alkyl ethers. Illustrative of the base compounds of the present invention represented by general formula (li) there can be mentioned: 2,7-bis[2-(diethylamino) ethoxy]-9-methoxyphenanthrene, 2,7-bis[3~ pyrrolidinyl) propoxy~-9-propoxyphenanthrene, 2,7-bis[2-(cyclohexylamino) ethoxy]-9-methoxyphenanthrene, 2,7-bis[4-(diallylamino) butoxy]-9-methoxyphenanthrene~ 3,8-bis[2-(diethylamino) ethoxy]-6-methoxyphenanthridine and 398-bis[4-(dimethyl-amino)butoxy]-6-ethoxyphenanthridine.
The expression "pharmaceutically acceptable acld addition salts" is Intended to apply ~o any non-toxic organic or inorganic acid addition salts of the base com-pounds represented by formulas (I) and (Il). Illustrative inorganic acids which form suitable salts include hydro-chloric, hydrobromic, sulfuric and phosphoric a~ids as ;
well as acid metal salts such as sodium monohydrogen orthophosphate and potassium hydrogen sulfate. Illustra-tive organic acids which form suitable salts include the mono, di and tricarboxylic acids. Illustrative of such acids are,ifor example, acetic, propionic, glycolic, lactic, pyruvic9 malonic, succinic, glutaricg fumaricg malicg tartaric, citric~ ascorbicg maleic, hydroxymaleic benzoic, p-hydroxybenzoicg phenylacetic, cinnamic, salicylic, 2-phenoxybenzoic and sulfonic acids such as methanesulfonic acid and 2-hydroxyethanesulfonic acid~
Either the mono or the di-acid salts can be formed, and such salts can exist in either a hydrated or a sub stantially anhydrous form.
In general the compounds of the present Tnvention , . , - . . ,, . :
.. . - . .. , , ... . . . . . ~,.
, : . . . .
.. : . . . :...... ~ , .. .
3LV4~39 are prepared From various 2,7-disubstituted-9-fluorenone precursors which are more fully described in U.S. Paten~
3,592,~19. The preparation of the instant compounds involv~s various ring expansions of the g-fluorenone nucleus to form the corresponding g-substituted phenan-threne, 6(5H) phenanthridlnone and 6H-dibenzo[b,d]pyran-6-one rings. More particularly, the reaction with diazo-methane results in the formation of the 2,7-bis bas;c ethers of 9-methoxyphenanthrene; the reaction with hydrazoic acid results in the formation of 3,8-bis basic ethers of 6(5H)-phenanthridinone; whereas the reaction with peracids or hydrogen peroxide results in the formation of the 3,8-bis basic ethers of 6H-dibenzo[bgd]pyran 6-one.
Cyclic aliphatic ketones are known to ring expand with diazomethane-to form larger cyclic aliphatic ketonesO
This reaction may also be conducted in some instances with aromatic ketones, producing a variety of homologous ring-enlarged ketones in addi~ion to the formation of enols, enol ethers and ethylene oxidesO Thusg for example, Schultz et alg J. Am. Chem SocO 62g 2902-4 (1940) reported the reaction of diazomethane with fluorenone to yield 5% of 9-phenanthrol, 30% of 9-methoxyphenanthreneg 1.5~ of di-9-phenanthryl etherg an unknown substance and 30~ of unchanged fluorenoneO In additiong substituents on the aromatic ring are known to change the ratio of the various products obtained as well as to give rise to additional isomericJ homologous ketones and side productsO ;
Thus, the application of the diazomethane reaction to the 2,7-bis-basic ethers of 9-fluorenone could not have been predicted and is not without difficultyO
Canada ~ ~f~ 39 The reaction is most frequently conducted by treat-ment of a methanolic solution of the carbonyl containing compound with an ethereal solution of diazomethane, either in the presence or absence of a catalyst. Alternatively, a solution of the carbonyl compound in methanol is treated with nitrosomethylurethane in the presence of a base. The diazomethane can be prepared either ex situ or in situ. Generally, applicants prefer to generate the diazomethane ex situ and to co-distill t-he diazomethane so produced with ether into a methanolic solution of the carbonyl containing compound. Suitable inert solvents which may also be utilized include such solvents as dioxane, benzene, toluene, chloroform and methylene chloride with ether-methanol being the solvent combination of choice. Additionally, methanol has been shown to have a high catalytic activity for dia~omethane ring expansion reactions. Catalysts which may be usefully employed in this reaction include trace amounts of metal salts such ;~
as zinc chloride or lithium chloride. A minimum of 2 equivalents of diazomethane are generally employed, one equivalent providing for the ring expansion, whereas the other equivalent competes with the starting material to form the corresponding 9-methoxyphenanthrene ethers (V).
In addition to a variety of side products which are formed, some of the intermediate 2,7-bis basic ethers of 9-phenanthrol (IV) also remain. The intermediates are readily separated from the reaction mixture in the form of their sodium salts. The 9-lower alkoxyphenanthrene derivatives (II), other than the 9-methoxyphenanthrene ~o derivatives, are prepared, in turn, by the reaction of the B
~ 4 ~ ~ 3~
9-phenanthrols (IV) with other lower diazoalkanes.
Due to the complex and wide variety of side reactions possible, no more than 8 equivalents of diazomethane are' useful in the preparation of the 2,7-bis-basic ethers g-methoxyphenanthreneO The reaction proceeds exothermall~
and is conducted at room temperature or below. The re-action takes place at a temperature range of from about -50C. to ambient temperatur-es with a temperature of 0C.
conveniently preferred. The reaction can be conducted for a period ranging anywhere from about,one hour to about seven days.
- The 9-phenanthrol derivatives~ which are also formed in the diazomethane ring expansion reaction,are readily freed of their nonacidic materials by extraction with chloroform from a strongly alkaline reactlon mixture.
The 9-phenanthrol derivatives which remain in the aqueous medium may then be removed by neutralization of the aqueous medium to a pH of about 9-10, and subsequently extracting the neutralized rnedium with chloroformO
The 9-phenanthrols so obtained can then be etherified by reacting with a lower diazoalkane to form the corresponding 9-lower alkoxy phenanthrene ethers~
The preparation of the 398-bis-baslc ethers of 6(5H) phenanthridinone (VI) is achieved via a modification of the so-called Schmidt reaction. Essentially, as applied here, this method inserts a hetero nitrogen atom via a ring expansion into the five membered fluoren-9-one ring using hydrazoic acid in the presence of a strong mineral acid such as sulfuric acid to form the six-membered ~0 heterocyclic ring of 6~5H)-phenanthridone. Hydra20ic ' ' . ' ' ! . ' ~ ' ' ' . ' ' ' . ' : ' acid is ~nown to react with simple cyclic ketones to form ring enlarged cyclic amides or lactams. Thus, for example, cyclohexanone reacts with hydrazoic acid yielding caprolactam. However, to applicants' knowledge, the use of hydrazoic acid on a 9-fluorenone nucleus containing a bis-basic ether side chain has not previously been reported. Approximately equimolecular quantities of hydrazoic acid are used inasmuch as an excess of hydrazoic acid encourages tetrazole formation. The reaction can be conducted by the addition of a solution of hydrazoic acid in an appropriate organic solvent to a solution of the fluoren-9-one. Alternatively, hydrazoic acid can be generated ln situ by the addition of sodium azide to the reaction mixture. Due to the extremely hazardous nature of hydrazoic acid, the use of sodium azide is preferred, thereby eliminating the necessity for the isolation of the toxic hydrazoic acid. This reaction is exothermic in nature, and consequently the reaction is best conducted by stirring with a suitable means for cooling. The reaction can be conducted at temperature ranges of from about -20C. to about 50C., and for periods of from about 30 minutes to about one week. Generally, a temperature of 0C. with a reaction period of about 1 hour is preferred as a matter of convenience. If the reaction appears to proceed slug-gishly, higher temperatures can be advantageously employed. Due to the rapidity of the reaction with hydrazoic acid, the reaction is conveniently controlled by the rate of addition of the hydrazoic acid or sodium azide to a stirred solution of the 9-fluorenone.
~' ' .
~ -, ~ ,. .' ' ' . . - , . . .
derivative dissolved or suspended in a suitable solvent.
Suitable solvents include sulfuric acid, chloroform, benzen~ dioxane and diethyl ether with trifluoroacetic acid h~ving been found to be particularly useful.
Generally, sodium azide is added in small increments to a trifluoroace~ic acid solution of the g-fluorenone ether derivat;ve, until no further evolution of nitrogen gas is observed. At this point the reaction is con-sidered to be complete for all practical purposes. An acid catalyst is also employed. Any strong acid may be used with concentrated sulfuric acid being the catalyst of choice. Isolation of the 3,8-bis basic ethers of 6(5H)-phenanthridinone so prepared is achieved using standard procedures apparent to those skilled in the art.
An alternative route to the preparation of the 6(5H)-phenanthridinone ethers is via a Beckmann rearrangement of the corresponding fluorenone oximes. Moore and Huntress, J. Am. Chem. Sc. 49, 2618 (1927), have demon~
strated the preparation of 7-nitro-6(5H)-phenanthridinone by the treatment of 2-n;trofluoren-9-one oxime with phosphorous pentachloride dissolved in phosphorous oxy- -chloride. In similar fashion the 3,8-bis basic ethers of 6(5H)-phenanthridinone can be prepared from the 2,7-bis-basic ethers of fluoren-9-one oxime as illustrated in the following reaction scheme:
- 19- ~
Z~39 N - ~ CHz ~ n - ~ O - ( C H2 ) - N /
POCl3 PCls NC1 Rl``
~N-(CH2)n-O~JO-(cH2)n-N~ ~ Ç .
Heat ~ . /R
- L ~R~ (CH2) -(CH2)n-O ~Cl Rl~
,~R 1 o~ 0~( CH2) n~N \ R ~ ¦
\N-(CH2)n-~R ~ OH
1~ ..
, R ~ ~ O- ( C H8 ~ ~ - N
,N_~HZ)n-o H
R O
M-~77 ~)4Z439 The first step in the preparation of the 3,8-bis-basic ethers o~ 6H-dibenzo[b,d~pyran-6-one involves the peracid or peroxide oxidation of a 2~7-bis(w~haloalkyl) ether of fluoren-9-one (Vll) under Baeyer-Villiger conditions. Essential 1YJ this reaction results ;n a ring expansion of the five-membered fluoren-9-one ring with the introduct10n of an oxygen atom to form a six-membered ring expanded lactone or 6H-dibenzo[b,d]
pyran-6-one nucleus. Thus, the oxidation of a 2,7-bis (w-haloalkyl~ ether of fluoren-g-one (Vll), found described in U.S. Patent ~5g2g819~ with a peracid or hydrogen peroxide in the presence of a strong acid~ such as sulfuric acid, results in the formation of a ~,8-bis (w-haloalkyl) ether of 6H-dibenzo[b,d]pyran-6-one (Vlll).
The 6H-dibenzo[bld]pyran (w-haloalkyl) ethe~s so pre-pared can then be condensed with an amine (IX) to form the desired 3,8-bis-basic ethers of 6H-dibenzo[bJdJpyran-6-one (X), the compounds of the present inventionO The -oxidation reaction is exothermic in nature and the temp-erature of the reaction mixture may be conveniently controlled by the rate of additi~n of the oxidizing agent.
The reaction temperatures ~ary from about -20 to 40C., with a temperature of about 25~C. being preferred~ ~ -Reaction times range anywhere from about ~0 minutes to about one week at the lower temperatures. Large excesses of peroxide are to be avoided inasmuch as there is always the hazard o-F peroxide oxidations occurring with explosive violenc;e. IF significant amounts of peroxide remain upon completion of the reaction, they can be decomposed using reducing agents such as sodium bisulfite or ferroùs ..
.
M-67rl~
1~4Z~39 sulfate. Generally, a two or three fold excess of hydrogen peroxide is satisfactory in carrying out the ring expansion.
The reaction is favored by polar solvents and proceeds in a variety of solvents such as sulfuric acid, acetic acid or acetic anhydride. The 3,8-bis(w-haloalkyl) ethers of 6H-dibenzo[b,d]pyran-6-one (Vlll) so prepared are sub-sequently condensed for the second step in the preparation of the 3,8-bis basic ethers of 6H-dibenzo[b,d]pyran-6-one ( X ) ~
The condensation reaction of the bis(~-haloalkyl) ethers (Vlli) with an amine.(IX) can be carrTed out using a variety of conditions. For example, the ethers can be heated together with a large excess of amine, the excess amine serving both as the reaction medium and the hydro-halide acceptorO This method is particularly suitàble for those amines which are readily available9 inasmuch.
as any excess amine can be readily removed from the ;~
reaction mixture via distillation at a reduced pressure or by steam distillation. Alternatively,.the w-haloalkyl ethers (Vlll) can be heated with an excess of~the amine in a suitable organic solvent. Suitable organic sol-vents include benzene, toluene, xylene and chlorobenzene, lower molecular weight alcohols such as methanolg ethanol -and isopropyl alcoholg or they may include .lower molec-ular weight ketones such as acetone and methyl ethyl ketone. The reaction of these ethers with an amine is generally promoted by the addition of either~ sodium or potassium iodide, the iodide being used in either cata-lytic or stoichiometric amounts. In some casesJ as when the amine is expensive or in short 5Upp 1 y, it may be .
:
~042~L39 advantageous to use only two equivalents of the amine for each equivalent of the ~-haloalkyl ether employed in the presence of an excess of e i ther powdered sodium or potassium carbonate as the acceptor for the hydrohalidç
that is ~enerated. In the case of volatile amines the condensation reaction can best be conducted under pressure in a suitable bomb or autoclave.
The compounds of the present invention are antiviral agents. Preferably they are administered to an animal host to prevent or inhibit viral infections. The term host refers to any viable biological material or intact animal including humans which is capable of inducing the formation of interferon and which serves as a support means for virus replicationO The host can be of animal ~ i or mammalian origin. Illustratively such hosts include birds, mice, rats, guinea pigs, gerb;ls, ferrets, dogs, cats, cows, horses and humans. Other viable biological mater;al such as used in the production of vaccines may also act as a host. Thus, tissue cultures prepared from organ tissues, such as mammalian kidney or lung tTssue9 as well as tissue cultures prepared from embryo tissue, such as obtained from amniotic cells or chick allantoic fluid, have been found to be useful hosts~
The treatment of virus infections for purposes of the present invention encompasses both the prevention and the inhibition of characteristic disease symptoms in a mammalian host susceptible to invasion by a pathogenic virus. Illustrative of mammalian virus infections which can be prevented or inhibited by the administration of ~0 the compounds of the present invention are infections -~3-- . .:: :. . . ~ .
M- ~77 ~ 0~43~
caused by picornaviruses, such as encephalomyocarditis virus; myxoviruses, such as influenza A2 (Jap/305) virus~
arboviruses; such as Semliki forest virus; the herpes group of viruses, including herpes simplex; and the po~-viruses, as for example vaccir~ia IHDo Thus9 for example~
the compounds of the present invent~on when administered orally or subcutaneously to mice in varying doses either shortly prior or subsequent to a fatal inoculation of a neurotropic virus such as encephalomyocarditis virus, having a LD50 anywhere from 5 to 50J delay or prevent completely the onset of death. Salts of these compounds are generally administered in compositions containing a 0.15% aqueous hydroxyethylcellulose vehicle, whereas the free base compounds are generally administered in compositions containing a 10~ aqueous surfactant vehicle in order to help solubilize the compound. In general~
ten mice are used for each treated group with an additional 20 mice serving as a control group. At the time of administration the test virus is titrated in order to determine the potency or LD50 for the particular virus pool used as a challenge. The control anima`ls are given a placebo containing the ident;cal volume of vehicle without, of course, the active ingredient. Because of the lethal ~ature of the test system employed, the anti-viral nature of the test compound is dramatically illustrated by a side by side comparison of the survival time of treated animals with the untreated control group of animalsO
Respiratory viruses, such as influenza ~2 ( Jap/305) ~0 virusJ which are also lethal to the test animals employed, ~ 4 Z ~ 3~
are administered via intranasal instillation. Animals infected in this manner have the active ingredients administered in the same manner as the test virus, and again a side by side comparison is made of the survivors of the animals ~reated with the untreated control animals.
Inexplicably, a mouse fcltally infected with encephalo-myocarditis or influenza virus occasionally survives with-out further treatment. This may be the result of a prior, interferon induced infection in the mouse9 or perhaps ~
due to some genetic factor or other natural defense mech- ' anism not presently understood. For this reason the control group selected is of sufficient size as to sta-tistically reduce to a negligible amount the influence of such a chancé survivor upon the test results.
The vaccinia test virus is typical of the dermato- ' ' trophic type viruses which respond to treatment with -compositions containing the compounds of the'instan~ `
Invention. The vaccinia virus generally produces a non-fatal infection in mice, producing characteristic tail lesions when the virus is subcutaneously'admi'nistered to the tail of the mouse. The instant compounds are adrninistered either orally or'subcutaneous-ly either prior '' ~;~''`
to or subsequent to the vaccinia infectionO Tail lesions are subjectively scored on the eighth day following infection against untreated animals which serve as a control group, The compounds of the present invention have been found to be effective in varying degrees against one or all of these test virus systems. ~ -The mode of activity of the active ingredients of the present invention is not rigorously defined. Inter .- . . . . .,~. . - ; .
; ~ ~ - . . , ~ .. , alia, the compounds ~ ~he present invention may induce the formation of interferon in a viable host. Interferon is a biological substance of unknown chemical structure, presumably proteinaceous in nature, which is produced by host cells in response to a viral infection. The inter feron so produced acts to induce a virus inhibiting su~
stance, which inhibits in some yet unknown manner the intracellular replication of the virus without appear-ing to hav`e any inactivation effect per se upon the virus itself. A few of the viruses susceptible to interferon replication inhibition are described in Horsfall and Tamm, "Viral and Rickettsial Infections of Man'~ 4th Edition (1965), J.B. Lippincott Company, pp. 328-9.
As previously indicated~ the compounds of the present invention may be prophylactically administered in order to prevent the spread of contagious viral diseases or they may be therapeutically administered to a host already infected intended for their curative effect. When admin-istered prophylactically3 it is pre~erred that the admin-istration be made within 0 to 96 hours prior to theinfection oF the host animal with a pathogenic virusi When the compounds of the present invention are admin-istered for their curative effect, it is preferred that they are admin'istered within about 1 or 2 days following infection of the host in order to obtain the maximum therapeutic effect.
The dosage to be administered will be dependent upon such parameters as the particular virus for which either treatment or prophyiaxis is desired, the species of animai ~0 involved, its age, health, weight, the extent of infection, ;. ' '' ' ~'~
Canada 1~?4i~439 concurrent treatmentg if ~ny~ frequency of treatment and the nature of the effect desired. A daily dose of the active ingredients will generally range from about 0.1 mg to about 500 mg per kg of body weight.. Illustratively dosage levels of the administered active ingredients for intravenous treatment range from about 0.1 mg to about 10 mg per kg of body weight; for intraperitoneal admin-istration range from about 0.1 mg to about 50 mg per kg of body weight; for subcutaneous administration range from about 0.1 my to about 250 mg per kg of body weight; for vral administration may be from about 0.1 mg to about 500 ~:
mg per kg of body weight; for intranasal instillation range .
from about 0.1 mg to about 10 mg per kg of body weight; an~
for aerosol inhalatian therapy, the range is generally from about 0.1 mg to about 10 mg per kg of body weight.
The novel compounds described herein can also be administered in various differPnt dosage unit forms, e.g., oral compositions such as tablets, capsules, dragees, :
lozenges, elixirs, emulsions, clear liquid solutions and suspensions; parenteral compositions such as intra-muscutar3 intravenous or intradermal preparations; and topical compositions, such as lotions, creams or ointments.
The amount of active ingredient contained in each dosage unit form will, o~ course, vary widely according to the ~.
particular dosage unit employed, the animal host being treated, and the nature of the treatment, i.e., whether prophylac~ic or therapeutic in nature. Thus, a particular dosage unlt may contain ~rom about 2.0 mg to over 3.0 9 of active ingredient in addition to the pharmaceutical excipients contained therein.
.. . .. . . . . . . .
.
. . .
~0~2439 The novel compounds described herein can be employed in conjunction or admixture with additional organic or inorganic pharmaceutical excipients. Suita'ble solid ex~ipients include gelatin, lactose, starches, magnesiu~
stearate and petrolatum. Suitable liquid excipients ~
include wa~er and alcohols such as ethanol, benzyl alcohol and the polyethylene alcohols either with or without the addition of a surfactantO In general~ the preferred liquid excipients particularly for injectable prep-arations, include water, saline solution, dextrose andglycol solutions such as an aqueous propylene glycol or an aqueous solution of polyethylene glycolO Liquid prep~. ' -arations to be used as sterile injectable'solutions will ordinarily contain from about 0.5% to about 25~
by weight, and preferably from about 1% t.o about 10~ .
by weight, of the active ingredient in solutionO In certain topical and parenteral preparationsl various ' oils are u~ilized as carriers or excipients.. Illustrative of such oils are mineral oils~ glyceride i!s. such as 20 ' lard oil, cod liver oil, peanut oil, sesame oil, corn oil and soybean oil. . . '.
-A suitable method of administrat'ion for the compounds of the present invention is orally either in:a soiid dose form such as a tablet or capsule, or in a liquid dose form such as an elixir, suspension, emulsion or.syrup. Ordi-narily the active ingredient comprises from about 0.5%
to about 10~ by weight of an oral liquid compositionO
In such compositions, the pharmaceutical carrier is generally aqueous in natureJ as for example,:aromatic ':
water, a sugar-based syrup or a pharmaceutical'mucilage.
,.. ,:~: - :
~ 342439 for insoluble compounds suspending agents may be added as well as agents to control viscosity, as for example, magnesium aluminum silicate or carboxymethylcellulose.
Buffers, preservatives9 emulsifying agents and other excipients can also be added.
For parenteral administration such as intramuscular intravenous or subcutaneous administration, the proportion of active ingredient ranges from about 0.05% to about 20%
by weight, and preferably from about 0.1~ to about 10%
by weight of the liquid COmpOSitiQn. In order to minimize or eliminate irritation at the site of injection, such compositions may contain a non-lonic surfactant having a hydrophile-lipophile balance (HLB) of from about 12 to about 17. The quantity of sùrfactant in such formu~
lations ranges from about 5% to about 15~ by weight. The -surfactant can be a single component having the above idèntified HLB, or a mixture of two or more components having the desired HLB. Illustrative of surfactants useful in parenteral formulations are the class of polyoxyethylene sorbitan fatty acid ethers as, for example, sorbitan monooleate and the high molecular weight adducts of ethylene oxide with a hydrophobic base, formed by the condensation of propylene oxide with propylene glycol.
The concentration of active ingredient contained in these various parenteral dosage unit forms varies over a broad range and comprises anywhere from about 0.05% to about 20% by weight of the total formulation, the remaining component or components comprising liquid pharmaceutical excipients previously mentioned.
The active ingredients of the present invéntion can , :-.,- . .
1t~4~39 also be admixed directly with animal feeds or incorpo-rated into the drinking water of animals. For most purposes, an amount of active ingredient is used which provides from about 0.0001~ to about 0.1% and preferably from abou-t 0.001% to about 0.02~ by weight of the active ingredient based upon the total weight o~ feed intake~
The active ingredients can be admixed in anima1 feed concentrates, suitable for use by farmers or livestock growers for incorporation in appropriate amounts with the final animal feeds~ These concentrates ordinarily .
comprise ~rom about 0.5% to about 95% by weight of the active ingredient compounded with a finely divided solid carrier or flour, such as wheat~ corn9 soybean or cottonseed flour. Depending upon the particular animal to be fed~ nutrients and fillers may also be added such as ground cereal, charcoal~ fuller's earth, oyster shells and finely divided attapulgite or bentoniteO ..
The active ingredients of the present invention can be packaged in a suitable pressurized container together ~ .
with an aqueous or volatile propellant for use as an aerosol. A suitable discharge valve is fitted to an. -opening in the container from which the active ingredi-ents may be conveniently dispensed in the form of a spray, liquid, ointment or foamO Additional adjuvants such as co-solvents, wetting agents and bactericides may be employed as necessary~ Normally, the propellant used is a liquified gaseous compound, preferably a mixture oF low molecular weight fluorinated hydrocarbons. These halo-alkanes are preferred bacause of their compatibility with the active ingredients of the present invention, and be~
~ V4Z~39 cause they are non-irritating when applied to skin surfac~s.
Other useful propellants include ethylene oxide, carbon dioxide, propane and nitrogen gas.
The invention described herein is more particularly illustrated by means of the following specific examples:
EXAMPLE I
.
2,7-Bis[2 (diethylamino)ethoxy]fluoren-9-one dihydrochloride A solution of [2-(diethylamino)ethyl]chloride obtained from 1505 9 (0.09 mole) of ~2-(diethylamino)ethyl]chloride hydrochloride in 100 ml of toluene (dried over molecular sieves) is added to a mixture of 6.4 g (0.03 mole) of 2J7-dihydroxy-fluoren-9-one and 3.3 g (o.o6 mole) of sodium methoxide in 200 ml of toluene (dried over molecular sieves). The resulting mixture is heated to its reflux temperature with stirrTng for a period of 3 hours. Upon cooling, the mixture is filtered to remove the precipi tated sodium chloride~ The toluene solution is washed 3 times with water, once with saturated sodium chlorid~
solution and dried over anhydrous magnesium sulfate~
This mixture is fiitered and the filtrate acidified to Congo Red with ethereal hydrogen chloride. The solid which precipitates is filtered, recrystallized from butanone with sufficient methanol being added to effect solution~ and the product dried at 100C. for 2~ hours under vacuum: m.p. 235-7C., ~ma 269~ and E 1~m 16000 EXAMPLE 1l ?,7-Bis~2-(diethylaminolethoxyl-9-methoxyphenanthrene A solution of 11.7 9 (0.028 mole) of 2,7-bis[2-(diethylamino)ethoxy]fluoren-g-one in 20 ml of ether and 50 ml of methanol is stirred and treated with approxi-' mately o.o8s mole of diazomethane which is co-distilled into the reaction mixture at 10-28C. with diethyl ether. The reaction mixture is stirred at room tempera-ture for 24 hours and most of the volatile materials are removed in vacuo. The residue is treated with a dilute solution of sodium hydroxide and extracted'several times with ether. The combined ether extracts are washed with water, dried over anhydroùs sodium~-sulfate, treate'd ' with charcoal, filtered and the product converted to -its hydrochloride salt by treatment with ethereal HCl.
This salt is then dissolved in 200 ml of ethanol and treated with 3.8 9 of G;rards Reagent T to allow for the subsequent removal of any unreacted starting material.
The resulting solution is refluxed for 3 hours, treated with 203 ml of a 5% sodium hydroxide solution and extracted with etherO The combined ether extracts are washed with water, dried and most of the volatile material removed. The resulting residue is~dissolved -~
in 40-60C~ petroleum ether, filtered, placed on an alumina'chromatographic column and eluted with 40-60Co petroleum ether. The initial eluate is collected, the volatiles removed and the 2,7-bis[2-(diethylamino)ethoxy]-9-methoxyphenanthrene so obtained is recrystallized from 40-60C~ petroleum ether and again from pentane solution, - -32- ' .
~042~39 ~
m.p. 63-5C., ~mae~H 259, and E 1~ 1590. -Following essentially the same procedure, but sub-stituting for the 2,7-bis[2-(diethylamino)ethoxy~
fluoren-g-one above) the appropriate molar equivalent quantities of 2J7-bis[2-(dibutylamino)ethoxy]fluoren-9~
one, 2~7-bis[2-(diisopropylamino)ethoxy]fluoren-9-one~ -2,7-bis[3-(dimethylamino)propoxy]fluoren-9-one and 2,7-bis[3-(dibutylamino)propoxy]fluoren-9-one, the following compounds are obtained: 2,7-bis[2-(dibutyl-amino)ethoxy]-g-methoxyphenanthrene, 2~7-bisC2-(diisopropyl-amino)ethoxy]-9-methoxyphenanthrene, 2,7-bisC~-(dimethyl-amino)propoxy]-g-methoxyphenanthrene and 2,7-bis[3-(dibutylamino)propoxy]-g-methoxyphenanthrene3 respectlvely.
' :
EXAMPLE lll ?,7-Bis(~piperidinopropox~) fluoren-9-one dihYdrochlorlde A mixture of 63.6 9 (0.30 mole) of 2J7-dihydroxy-~luoren-9-one, 188 9 (0.95 mole) of 1-(3-chloropropyl) piperidine hydrochloride, 132 9 (2.0 mole) of 85%
potassium hydroxide in 900 ml of toluene and 300 ml of water is refluxed with vigorous stirring for 20 hoursO
The layers are separated upon cooling and the organic layer is washed 3 times with water, once with a saturated solution of sodium chloride and dried over anhydrous magnesium sulfate. The mixture is filtered and the solvent removed tn vacuo. The residue is taken up in isopropyl alcohol and acidified to Congo Red with ethereal hydrogen chloride. The solid which precip;tates ~3-~ - - - , . i . .. . .. . . .. . .
Canada
4'~439 ts filtered, recrystallized from a mixture of 3 parts isopropyl alcohol to 1 part methanol, and the 2,7-bis (3-piperidinopropoxy)fluoren-9~one dihydrochloride so prepared ts dried at 100C. for 24 hours under vacuum, m.p. 279.5-80.5C., ~Hax 270, and E 1~m 1370.
EXAMPLE IV
9-Methoxy-2,7-bis(3-piperidinopropox~) phenanthrene A solution of 4.7 g (0.01 mole) of 2,7-bls(~-piper-idinopropoxy)fluoren-9-one in methanol is stirred and treated with approximately o.o8s mole of diazomethane by co-distillation with ether into the reaction mixture at a temperature of -15~ to -31C. The reaction mixture is allowed to warm slowly to room temperature overnight.
The volatile materials are removed on the s~eam bath in vacuo and the residue tritura~ed with pentane to induce crystallization. The resulting solid is dissolved in a mixture of pentane and ether, washed several times with a dilute solution of sodium hydroxide, washed with water, dried over anhydrous sodium sulfate, treated with charcoal, filtered and most of the volatile solvents removed from the filtrate. The residue is recrystallized from pentane and again from a methanol-water mixture to yield the desired 9-methoxy-2,7-bis(3-piperidinopropoxy) phenanthrene: m.p. 118.5-9.5C., ~mMaeH 259, and E 1%m 14~0.
Following essen~ially the same procedure, but sub-stituting for the 2,7-bis(~-piperidinopropoxy)fluoren-9-"~
~,~ j .. . - ~ .
Canada ~ 39 one above, the appropriate molar equivalent quantities of 2,7-bis[2~ pyrrolidinyl)ethoxy]fluoren-9-one or 2,7-bis(4-morpholinobutoxy)~luoren-9-one results in the formation of 9-methoxy-2,7-bis~2-(1-pyrrolidinyl)ethoxy]
phenanthrene and 9-methoxy-2,7-bis(4-morpholinobutoxy) phenanthrene, respectively.
EXAMPLE V
~`.
2,,7-Bis~2-(dimethylamino)ethoxyl - ' -9-phenanthrol A solution of 8.1 9 (0.023 mole) of 2,7-bis[2-(dimethylamino)ethoxy]fluoren-9-one in 50 ml of methanol and 25 ml of ether is stirred and treated with 0.5 gram of finely powdered sodium carbonate. To this mixture is added 75 g (0.06 mole) of ethyl N-methyl-N-nitrosocarbamate in 20 ml of methanol over a period of 2 hours. The temp-erature of this reaction mixture is kept below ~0C. for a period of 24 hours. The react10n mixture is then freed of most of the volatile materials In vacuo, dissolved in an aqueous solution of hydrochloric acid, and extracted several times with diethyl ether. The resulting aqueous solution is made strongly alkaline with an aqueous sodium hydroxide solution and extracted with ether. The remaining aqueous solution is neutralized to an approximate pH of 10 using a 10~ hydrochloric acid solution and extracted with ether. Evaporation o~ the combined ether extracts ~n vacuo and recrystallization from a methanol-water mixture yields the desired 2,7-bis[2-(d1methylamino) ethoxy]-9-phenanthrol.
"' ' Canada ~ Z 4 ~9 Preparation o~ the 9~1Oweralkoxy ethers is accomplished by further reacting a methanolic solution of 2,7-bis[2-(dimethylamino)ethoxy]-9-phenanthrol with a diazoloweralkane, such as diazoe~hane and diazGpro-pane. The resulting 2,7-bis~2-(dimethylamino)ethoxy]-9-ethoxyphenanthrene and 2~7-bis[2-(dimethylamino)ethoxy]-9-propoxyphenanthrene so prepared can be isolated by removal of ~he volatile materials In vacuo and recrys~al-izing the residue from aqueous methanol.
EXAMPLE_VI
3,8-Bis~?-(diethylamino)ethoxYl -6(5H)-phenanthridinone dihydrochloride Using the product prepared in accordance with Example 1, 10 9 (0.027 mole) of 2,7-bis[2-(diethylamino)ethoxy~
fluoren-9-one dihydrochloride is dissolved in 75 ml of trifluoroacetic acid and chilled to a temperature of from 0-5~C. Sodium azide, ~.5 9, is added in increments with stirring followed by 10 ml of sulfuric acid which is added in a dropwise fashion. Stirring and cooling is continued for an additional hour and the solution made alkaline with an excess of a 20% potassium hydroxide solution. The basic reaction mixture is extracted several times with methylene chloride, the extracts are combined, washed with water, dried over anhydrous sodium sulfate, filtered and the filtrate evaporated in vacuo.
The residue is dissolved in ether and acidified with -ethereal hydrogen chloride. RecrystallTzation of the residue from a mixture of methanol-butanone yielded .
. . ., .~ .
.. . ..
, . `
~ 4'~43~
3,8-bis[2-(diethyiamino)ethoxy]-6(5H)-phenanthridinone as the dihydrochloride salt having a m.p. of 250-2C., ~EtOH 230, and E 1c~m 885.
Following essentially -~he same procedure but sub-stituting the appropriate molar equivalent amounts of 2,7-bis(2-piperidinoethoxy)fluoren-9-one dihydrochlorideJ
2,7-bis[3-(dimethylamino)propoxy]fluoren-9-one dihydro-chloride and 2,7-bis[2-(diallylamino)ethoxy]fluoren-9-one dihydrochloride for the 2,7-bis[2-(diethylamino)ethoxy]
fluoren-9-one dihydrochloride results in the formation of the following compounds, respectively: 3,8-bis(2-piper-idinoethoxy)-6(5H)-phenanthridinone dihydrochloride, 3,8-bis[3-(dimethylamino)propoxy]-6(5H)-phenanthridinone dihydrochloride and 3~8-bis[2-(dlallylamino)ethoxy]-6(5H)-phenanthridinone dihydrochloride.
EXAMPLE Vl~
3,8-Bis~?~ dimethylamino)ethoxyL
-6H-dibenzo~b,dlpyran-6-one dihydrochloride A solution of 13.5 9 (0.04 mole) of 2,7-bis(2-chloro-ethoxy)fluoren-9-one in 100 ml of concentrated sulfuric acid is slowly reacted with 12 ml of a 30% hydrogen per-oxide solution at room temperatureO The reaction which is strongly exothermic is controlled by cooling the stirred reaction mixture in an ice-water bath~ The cooled reaction mixture is poured onto 500 ml of an ice-water mixture and the resulting 3,8-bis(2-chloroethoxy)-6H-dibe~zo [b,d~pyran-6-one which separates is isolated.
A mixture of 4.6 9 (0.013 mole) o~ 3,8-bis(2-chloro-~ M-677 Z~39 ethoxy)-6H-dibenzo[b,d]pyran-6-one so obtained~ 2 g of potassium iodide and 100 ml of a l~o~ aqueous dimethyl-amine solution contained in 50 ml of tetrahydrofuran is heated in a Parr pressure reactor with stirring at 120C.
for sixteen hours. The reaction vessel is cooled and most of the volatile materials are removed in vacuo.
The residue is treated with an aqueous sodium hydroxide solution and extracted with ether. The ether solution is washed twice with water, once with a saturated solution of sodium chloride, dried over anhydrous mag-nesium sulfate and treated with ethereal hydrogen chloride. The product which separates is then recrys-talized from a methanol-anhydrous ether mixture to yield the desired 3,8-bis[2-(dimethylamino)ethoxy]-6H-dibenzo [b,d]pyran-~-one dihydrochloride.
EXAMPLE Vlll .
The following Example is illustrative of the anti-viral activity for the compounds of the present invention.
Thirty mice each weighing approximately 12 to 15 gms are divided into two groups, a control group containing 20 animals and a test group o~ 10 animals. All of the animals are challenged with a fatal dose (28LD50) of encephalomyocarditis virus. The test group of an7mals are treated both prophylactically and therapeutically using a parenteral composition containing 3,8-bis[2-diethylamino)ethoxy]-6(5H)-phenanthridinone dihydro-chloride as the active ingredient dissolved in an aqueous solution of 0.15% hydroxyethylcellulose. The compo--3~-.. ..
;.. .
sition contains the active ingredient in an amount such that each dosage contains 0.25 ml which is equiva1ent to a dose level of 50 mg per kg. The control group receives a subcutaneous placebo containing the same vol4me of vehicle without, of course, the active ingredient.
Observations over a ten day period show a termination of all the control animals within a period of from ~ to 5 days, with the treated group of animals surviving for a - s~atistically longer period of time.
EXAMPLE IX
Preparation of a tablet formulation An illustrative preparatlon of 10gOOO tabletsg each containing 100 mg of 3~8-bis[2-(diethylamino)etho~y]-6(5H)-phenanthridinone is prepared as follows:
Gm.
(a) 3/8-bis[2-(diethylamino~etho~y]
-6(5H)-phenanthridinone ......... 0..... O. 1000 (b) Lactose ....... ;.....,........................... 1000 (c) Starch paste (10% w/v starch in water) .......................... 100 (d) Starch .................................... 32.5 (e) Calcium stearate ......................... 0 6.5 The active ingredient is uniformly mixed with the lactose and granulated by the addition of the starch paste.
The granules which form are dried at 120F. for 20 hours and forced through a No. 16 screen. The granules are lubricated by the addition of the starch and calcium stearate and compressed into tablets.-Each tablet so prepared contains 100 mg of the active ingre~iènt.
,"~
-39~
.
EXAMPLE X
Preparation of_a ca~ le formulation An illustrative composition for the preparation of 1000 two-piece hard gelatin capsules, each capsule con-taining 100 mg of 2,7-bis[2-(diethylamino)ethoxy]-9-methoxyphenanthrene is prepared as follows:
Gm.
(a) 2,7-bis[2-(diethylamino)ethoxy]
-9-methoxyphenanthrene .................. ~ 100 (b) Corn starch O~ D 150 (c) Magnesium stearate ....... ~............... ...25 (d) 1000 Hard gelatin capsules The finely powdered ingredients are mixed until uniformly dispersed and then filled into hard shelled gelatin capsules of the appropriate size.
In a similar fashion, soft gelatin capsules may be prepared in which the above composition can be granu-lated, slugged or directly compressed in a rotary die or ~;
plat~ mold in which the soft gelatin capsule is formed. -~
Alternatively, the above excipients may be omitted and the active ingredient dispensed as a powder directly into the soft gelatin capsule.
EXAMPLE Xl , . _ .
- Preparation of an oral s~up formulation A 2~ weight per volume syrup of 3,8-bis(4-piperidino-butoxy)-6(5H)-phenanthridinone dihydrochloride is pre-- pared in accordance with the usual pharmaceutical , , , . .. - - . . .
.. , , .. .:
.. , . ~
~0~2439 techniques which has the following formula:
Gm.
(a) Finely divided 3,8-bis(4-piper-idinobutoxy)-6(5H)-phenanthridinone dihydrochloride ....................... ~ 2.0 (b) Sucrose ................................. 33.3 (c) Chloroform .............................. 0.25 (d) Sodium benzoate .......................... 0.4 (e) Methyl p-hydroxybenzoate ............. ,. 0.02 (f) Vanillin ................................ 0.04 (g) Glycerol ............................... 0 1o5 (h) Purified water to 100..0 ml EXAMPLE Xll Preparation of ointment formulation One thousand grams of an ointment for topical appli-cation containing 1.0% of 3,8-bis[2-(diethylamino)ethoxy]
-6(5H)-phenanthridinone dihydrochloride is prepared from the following ingredients:
Gm.
-(a) 3,8-bis[2-(diethylamino)ethoxy]
-6(5H)-phenanthridinone dihydro-chloride ................................. 10 (b) Li~qht liquid pe$rolatum ~................ 250 (c) Wool fat .......;.............................. 200 (d) White petrolatum q.s. ad 1000 The wool fat, white petrolatum and 200 gms of the - light liquid petrolatum are liquified and held at 110DF.
The active ingredient is mixed with the remaining liquid petrolatum and passed through a colloid mill. After : : passing through the mill, the mixture is stirred into the . . - .. . .. .
, M-677 1~34243g melt, and the melt is permitted to cool with continued stirring until congealed.
EXAMPLE Xlll Preparation of a parenteral emulsion formulatlo,n An illustrative composition for an emulsion which is parenterally injectable is as ~ollows:
Each ml Contains Ingredients Amount 50 mg ~,8-bis[2-(diethylamino)ethoxy]
-6(5H)-phenanthridinone 1.000 9 100 mg Polyoxyethylene sorbi$an monooleate 2.000 9 o. oo64 Sodium chloride 0.128 9 ,~
Water for injection q.sO 20.000 ml ,' The parenteral composition is prepared by dissolving o.64 9 of sodium chloride in 100 ml of water suitable for injectionO The polyoxyethylene sorbitan monooleate is mixed with the active ingredient, and an amount of the previously prepared aqueous sodium chloride solution added which is sufficient to bring the total volume to ~' 20 ml. The resulting solution is shaken and autoclaved for 20 minutes at 110C. at 15 p.s.i.g. steam pressure.
The composition can be dispensed in a single ampule for use in multiple dosages or it can be dispensed in 10 or 20 individual ampules for use as a single dosage unit~
: .
,
EXAMPLE IV
9-Methoxy-2,7-bis(3-piperidinopropox~) phenanthrene A solution of 4.7 g (0.01 mole) of 2,7-bls(~-piper-idinopropoxy)fluoren-9-one in methanol is stirred and treated with approximately o.o8s mole of diazomethane by co-distillation with ether into the reaction mixture at a temperature of -15~ to -31C. The reaction mixture is allowed to warm slowly to room temperature overnight.
The volatile materials are removed on the s~eam bath in vacuo and the residue tritura~ed with pentane to induce crystallization. The resulting solid is dissolved in a mixture of pentane and ether, washed several times with a dilute solution of sodium hydroxide, washed with water, dried over anhydrous sodium sulfate, treated with charcoal, filtered and most of the volatile solvents removed from the filtrate. The residue is recrystallized from pentane and again from a methanol-water mixture to yield the desired 9-methoxy-2,7-bis(3-piperidinopropoxy) phenanthrene: m.p. 118.5-9.5C., ~mMaeH 259, and E 1%m 14~0.
Following essen~ially the same procedure, but sub-stituting for the 2,7-bis(~-piperidinopropoxy)fluoren-9-"~
~,~ j .. . - ~ .
Canada ~ 39 one above, the appropriate molar equivalent quantities of 2,7-bis[2~ pyrrolidinyl)ethoxy]fluoren-9-one or 2,7-bis(4-morpholinobutoxy)~luoren-9-one results in the formation of 9-methoxy-2,7-bis~2-(1-pyrrolidinyl)ethoxy]
phenanthrene and 9-methoxy-2,7-bis(4-morpholinobutoxy) phenanthrene, respectively.
EXAMPLE V
~`.
2,,7-Bis~2-(dimethylamino)ethoxyl - ' -9-phenanthrol A solution of 8.1 9 (0.023 mole) of 2,7-bis[2-(dimethylamino)ethoxy]fluoren-9-one in 50 ml of methanol and 25 ml of ether is stirred and treated with 0.5 gram of finely powdered sodium carbonate. To this mixture is added 75 g (0.06 mole) of ethyl N-methyl-N-nitrosocarbamate in 20 ml of methanol over a period of 2 hours. The temp-erature of this reaction mixture is kept below ~0C. for a period of 24 hours. The react10n mixture is then freed of most of the volatile materials In vacuo, dissolved in an aqueous solution of hydrochloric acid, and extracted several times with diethyl ether. The resulting aqueous solution is made strongly alkaline with an aqueous sodium hydroxide solution and extracted with ether. The remaining aqueous solution is neutralized to an approximate pH of 10 using a 10~ hydrochloric acid solution and extracted with ether. Evaporation o~ the combined ether extracts ~n vacuo and recrystallization from a methanol-water mixture yields the desired 2,7-bis[2-(d1methylamino) ethoxy]-9-phenanthrol.
"' ' Canada ~ Z 4 ~9 Preparation o~ the 9~1Oweralkoxy ethers is accomplished by further reacting a methanolic solution of 2,7-bis[2-(dimethylamino)ethoxy]-9-phenanthrol with a diazoloweralkane, such as diazoe~hane and diazGpro-pane. The resulting 2,7-bis~2-(dimethylamino)ethoxy]-9-ethoxyphenanthrene and 2~7-bis[2-(dimethylamino)ethoxy]-9-propoxyphenanthrene so prepared can be isolated by removal of ~he volatile materials In vacuo and recrys~al-izing the residue from aqueous methanol.
EXAMPLE_VI
3,8-Bis~?-(diethylamino)ethoxYl -6(5H)-phenanthridinone dihydrochloride Using the product prepared in accordance with Example 1, 10 9 (0.027 mole) of 2,7-bis[2-(diethylamino)ethoxy~
fluoren-9-one dihydrochloride is dissolved in 75 ml of trifluoroacetic acid and chilled to a temperature of from 0-5~C. Sodium azide, ~.5 9, is added in increments with stirring followed by 10 ml of sulfuric acid which is added in a dropwise fashion. Stirring and cooling is continued for an additional hour and the solution made alkaline with an excess of a 20% potassium hydroxide solution. The basic reaction mixture is extracted several times with methylene chloride, the extracts are combined, washed with water, dried over anhydrous sodium sulfate, filtered and the filtrate evaporated in vacuo.
The residue is dissolved in ether and acidified with -ethereal hydrogen chloride. RecrystallTzation of the residue from a mixture of methanol-butanone yielded .
. . ., .~ .
.. . ..
, . `
~ 4'~43~
3,8-bis[2-(diethyiamino)ethoxy]-6(5H)-phenanthridinone as the dihydrochloride salt having a m.p. of 250-2C., ~EtOH 230, and E 1c~m 885.
Following essentially -~he same procedure but sub-stituting the appropriate molar equivalent amounts of 2,7-bis(2-piperidinoethoxy)fluoren-9-one dihydrochlorideJ
2,7-bis[3-(dimethylamino)propoxy]fluoren-9-one dihydro-chloride and 2,7-bis[2-(diallylamino)ethoxy]fluoren-9-one dihydrochloride for the 2,7-bis[2-(diethylamino)ethoxy]
fluoren-9-one dihydrochloride results in the formation of the following compounds, respectively: 3,8-bis(2-piper-idinoethoxy)-6(5H)-phenanthridinone dihydrochloride, 3,8-bis[3-(dimethylamino)propoxy]-6(5H)-phenanthridinone dihydrochloride and 3~8-bis[2-(dlallylamino)ethoxy]-6(5H)-phenanthridinone dihydrochloride.
EXAMPLE Vl~
3,8-Bis~?~ dimethylamino)ethoxyL
-6H-dibenzo~b,dlpyran-6-one dihydrochloride A solution of 13.5 9 (0.04 mole) of 2,7-bis(2-chloro-ethoxy)fluoren-9-one in 100 ml of concentrated sulfuric acid is slowly reacted with 12 ml of a 30% hydrogen per-oxide solution at room temperatureO The reaction which is strongly exothermic is controlled by cooling the stirred reaction mixture in an ice-water bath~ The cooled reaction mixture is poured onto 500 ml of an ice-water mixture and the resulting 3,8-bis(2-chloroethoxy)-6H-dibe~zo [b,d~pyran-6-one which separates is isolated.
A mixture of 4.6 9 (0.013 mole) o~ 3,8-bis(2-chloro-~ M-677 Z~39 ethoxy)-6H-dibenzo[b,d]pyran-6-one so obtained~ 2 g of potassium iodide and 100 ml of a l~o~ aqueous dimethyl-amine solution contained in 50 ml of tetrahydrofuran is heated in a Parr pressure reactor with stirring at 120C.
for sixteen hours. The reaction vessel is cooled and most of the volatile materials are removed in vacuo.
The residue is treated with an aqueous sodium hydroxide solution and extracted with ether. The ether solution is washed twice with water, once with a saturated solution of sodium chloride, dried over anhydrous mag-nesium sulfate and treated with ethereal hydrogen chloride. The product which separates is then recrys-talized from a methanol-anhydrous ether mixture to yield the desired 3,8-bis[2-(dimethylamino)ethoxy]-6H-dibenzo [b,d]pyran-~-one dihydrochloride.
EXAMPLE Vlll .
The following Example is illustrative of the anti-viral activity for the compounds of the present invention.
Thirty mice each weighing approximately 12 to 15 gms are divided into two groups, a control group containing 20 animals and a test group o~ 10 animals. All of the animals are challenged with a fatal dose (28LD50) of encephalomyocarditis virus. The test group of an7mals are treated both prophylactically and therapeutically using a parenteral composition containing 3,8-bis[2-diethylamino)ethoxy]-6(5H)-phenanthridinone dihydro-chloride as the active ingredient dissolved in an aqueous solution of 0.15% hydroxyethylcellulose. The compo--3~-.. ..
;.. .
sition contains the active ingredient in an amount such that each dosage contains 0.25 ml which is equiva1ent to a dose level of 50 mg per kg. The control group receives a subcutaneous placebo containing the same vol4me of vehicle without, of course, the active ingredient.
Observations over a ten day period show a termination of all the control animals within a period of from ~ to 5 days, with the treated group of animals surviving for a - s~atistically longer period of time.
EXAMPLE IX
Preparation of a tablet formulation An illustrative preparatlon of 10gOOO tabletsg each containing 100 mg of 3~8-bis[2-(diethylamino)etho~y]-6(5H)-phenanthridinone is prepared as follows:
Gm.
(a) 3/8-bis[2-(diethylamino~etho~y]
-6(5H)-phenanthridinone ......... 0..... O. 1000 (b) Lactose ....... ;.....,........................... 1000 (c) Starch paste (10% w/v starch in water) .......................... 100 (d) Starch .................................... 32.5 (e) Calcium stearate ......................... 0 6.5 The active ingredient is uniformly mixed with the lactose and granulated by the addition of the starch paste.
The granules which form are dried at 120F. for 20 hours and forced through a No. 16 screen. The granules are lubricated by the addition of the starch and calcium stearate and compressed into tablets.-Each tablet so prepared contains 100 mg of the active ingre~iènt.
,"~
-39~
.
EXAMPLE X
Preparation of_a ca~ le formulation An illustrative composition for the preparation of 1000 two-piece hard gelatin capsules, each capsule con-taining 100 mg of 2,7-bis[2-(diethylamino)ethoxy]-9-methoxyphenanthrene is prepared as follows:
Gm.
(a) 2,7-bis[2-(diethylamino)ethoxy]
-9-methoxyphenanthrene .................. ~ 100 (b) Corn starch O~ D 150 (c) Magnesium stearate ....... ~............... ...25 (d) 1000 Hard gelatin capsules The finely powdered ingredients are mixed until uniformly dispersed and then filled into hard shelled gelatin capsules of the appropriate size.
In a similar fashion, soft gelatin capsules may be prepared in which the above composition can be granu-lated, slugged or directly compressed in a rotary die or ~;
plat~ mold in which the soft gelatin capsule is formed. -~
Alternatively, the above excipients may be omitted and the active ingredient dispensed as a powder directly into the soft gelatin capsule.
EXAMPLE Xl , . _ .
- Preparation of an oral s~up formulation A 2~ weight per volume syrup of 3,8-bis(4-piperidino-butoxy)-6(5H)-phenanthridinone dihydrochloride is pre-- pared in accordance with the usual pharmaceutical , , , . .. - - . . .
.. , , .. .:
.. , . ~
~0~2439 techniques which has the following formula:
Gm.
(a) Finely divided 3,8-bis(4-piper-idinobutoxy)-6(5H)-phenanthridinone dihydrochloride ....................... ~ 2.0 (b) Sucrose ................................. 33.3 (c) Chloroform .............................. 0.25 (d) Sodium benzoate .......................... 0.4 (e) Methyl p-hydroxybenzoate ............. ,. 0.02 (f) Vanillin ................................ 0.04 (g) Glycerol ............................... 0 1o5 (h) Purified water to 100..0 ml EXAMPLE Xll Preparation of ointment formulation One thousand grams of an ointment for topical appli-cation containing 1.0% of 3,8-bis[2-(diethylamino)ethoxy]
-6(5H)-phenanthridinone dihydrochloride is prepared from the following ingredients:
Gm.
-(a) 3,8-bis[2-(diethylamino)ethoxy]
-6(5H)-phenanthridinone dihydro-chloride ................................. 10 (b) Li~qht liquid pe$rolatum ~................ 250 (c) Wool fat .......;.............................. 200 (d) White petrolatum q.s. ad 1000 The wool fat, white petrolatum and 200 gms of the - light liquid petrolatum are liquified and held at 110DF.
The active ingredient is mixed with the remaining liquid petrolatum and passed through a colloid mill. After : : passing through the mill, the mixture is stirred into the . . - .. . .. .
, M-677 1~34243g melt, and the melt is permitted to cool with continued stirring until congealed.
EXAMPLE Xlll Preparation of a parenteral emulsion formulatlo,n An illustrative composition for an emulsion which is parenterally injectable is as ~ollows:
Each ml Contains Ingredients Amount 50 mg ~,8-bis[2-(diethylamino)ethoxy]
-6(5H)-phenanthridinone 1.000 9 100 mg Polyoxyethylene sorbi$an monooleate 2.000 9 o. oo64 Sodium chloride 0.128 9 ,~
Water for injection q.sO 20.000 ml ,' The parenteral composition is prepared by dissolving o.64 9 of sodium chloride in 100 ml of water suitable for injectionO The polyoxyethylene sorbitan monooleate is mixed with the active ingredient, and an amount of the previously prepared aqueous sodium chloride solution added which is sufficient to bring the total volume to ~' 20 ml. The resulting solution is shaken and autoclaved for 20 minutes at 110C. at 15 p.s.i.g. steam pressure.
The composition can be dispensed in a single ampule for use in multiple dosages or it can be dispensed in 10 or 20 individual ampules for use as a single dosage unit~
: .
,
Claims (12)
1. A process for preparing a 2,7-bis-basic ether of 9-phenanthrol having the formula wherein n is an integer of from 2 to 6, R and R1 are each selected from the group consisting of lower alkyl having from 1 to 6 carbon atoms and R and R1 when taken together with the nitrogen atom to which they are attached represent the piperidino radical, and R3 is hydrogen or methyl, or a pharmaceutically acceptable acid addition salt thereof;
which comprises reacting a 2,7-bis-basic ether of fluoren-9-one having the formula wherein the symbols n, R and R1 are as defined above;
reacting said fluoren-9-one with diazomethane; and isolating the resulting product therefrom.
which comprises reacting a 2,7-bis-basic ether of fluoren-9-one having the formula wherein the symbols n, R and R1 are as defined above;
reacting said fluoren-9-one with diazomethane; and isolating the resulting product therefrom.
2. A 2,7-bis-basic ether of 9-phenanthrol having the formula wherein n is an integer of from 2 to 6, R and R1 are each selected from the group consisting of lower alkyl having from 1 to 6 carbon atoms and R and R1 when taken together with the nitrogen atom to which they are attached repre-sent the piperidino radical, and R3 is hydrogen or methyl, or a pharmaceutically acceptable acid addition salt thereof, when prepared by the process of claim 1.
3. A process for the preparation of 2,7-bis[2-(diethylamino)ethoxy]-9-methoxyphenanthrene which comprises reacting 2,7-bis[2-(diethylamino)ethoxy]fluoren-9-one with diazomethane.
4. 2,7-Bis[2-(diethylamino)ethoxy]-9-methoxyphenan-threne or a pharmaceutically acceptable acid addition salt thereof when prepared by the process of claim 3.
5. A process for preparing a 3,8-bis-basic ether of 6(5H)-phenanthridinone having the formula wherein n is an integer of from 2 to 6, R and R1 are each selected from the group consisting of lower alkyl having from 1 to 6 carbon atoms and R and R1 when taken together with the nitrogen atom to which they are attached repre-sent the piperidino radical, or a pharmaceutically acceptable acid addition salt thereof, which comprises reacting a 2,7-bis-basic ether of fluoren-9-one having the formula wherein the symbols n, R and R1 are used as defined above;
reacting said fluoren-9-one with hydrazoic acid in the presence of a strong mineral acid; and isolating the resulting product therefrom.
reacting said fluoren-9-one with hydrazoic acid in the presence of a strong mineral acid; and isolating the resulting product therefrom.
6. A 3,8-bis-basic ether of 6(5H)-phenanthridinone having the formula wherein n is an integer of from 2 to 6, R and R1 are each selected from the group consisting of lower alkyl having from 1 to 6 carbon atoms and R and R1 when taken together with the nitrogen atom to which they are attached represent the piperidino radical, or a pharmaceutically acdeptable acid addition salt thereof, when prepared by the process of claim 5.
7. A process for the preparation of 3,8-bis 2-(diethylamino)ethoxy -6(5H)-phenanthridinone which comprises reacting 2,7-bis 2-(diethylamino) ethoxy fluoroen-9-one with hydrazoic acid in the presence of a strong mineral acid.
8. 3,8-Bis 2-(diethylamino)ethoxy -6(5H)-phenanthri-dinone or a pharmaceutically acceptable acid addition salt thereof when prepared by the process of claim 7.
9. A process for preparing a 3,8-bis-basic ether of 6H-dibenzo b,d pyran-6-one having the formula wherein n is an integer of from 2 to 6, R and R1 are each selected from the group consisting of lower alkyl having from 1 to 6 carbon atoms and R and R1 when taken together with the nitrogen atom to which they are attached represent the piperidino radical, or a pharmaceutically acceptable acid addition salt thereof, which comprises reacting a 2,7-bis-( -haloalkoxy)fluoren-9-one having the formula wherein the value above and Hal is chlorine, bromine, or iodine, reacting said 2,7-bis( -haloalkoxy)fluoren-9-one with a peracid or with hydrogen peroxide in the pre-sence of a strong acid catalyst to form a 3,8-bis( -haloalkoxy)6H-dibenzo b,d pyran-6-one; condensing said dibenzo b,d pyran-6-one wich an amine having the formula wherein R and R1 have the values previously described; and isolating the resulting product therefrom.
10. A 3,8-bis-basic ether of 6H-dibenzo[b,d]pyran-6-one having the formula wherein n is an integer of from 2 to 6, R and R1 are each selected from the group consisting of lower alkyl having from 1 to 6 carbon atoms and R and R1 when taken together with the nitrogen atom to which they are attached represent the piperidino radical, or a pharmaceutically acceptable acid addition salt thereof, when prepared by the process of claim 9.
11. A process for preparing a bis-basic ether of 9-substituted phenanthrene or 10-oxa and 10-aza deriva-tives thereof having the formulae:
or wherein n is an integer of from 2 to 6, R and R1 are each selected from the group consisting of lower alkyl having from 1 to 6 carbon atoms and R and R1 when taken together with the nitrogen atom to which they are attached represent the piperidino radical, R2 is hydrogen or methyl, Z is selected from the group consisting of oxygen, imino and methylene, Y is nitrogen or methylidene, or a pharma-ceutically acceptable acid addition salt thereof, which comprises a process selected from:
(a) whenever Z is methylene, reacting a 2,7-bis-basic ether of fluoren-9-one having the formula with diazomethane, and isolating the resulting product therefrom;
(b) whenever Z is imino and R2 is hydrogen, reacting a 2,7-bis-basic ether of fluoren-9-one having the formula with hydrazoic acid in the presence of a strong mineral acid, and isolating the resulting product therefrom; or (c) whenever Z is oxygen and R2 is hydrogen, reacting a 2,7-bis(.omega.-haloalkoxy)fluoren-9-one having tile formula with a peracid or with hydrogen peroxide in the presence of a strong acid catalyst to form a 3,8 bis(.omega.-haloalkoxy)6H-dibenzo[b,d]pyran-6-one, condensing said dibenzo[b,d]pyran-6-one with an amine having the formula and isolating the resulting product therefrom.
or wherein n is an integer of from 2 to 6, R and R1 are each selected from the group consisting of lower alkyl having from 1 to 6 carbon atoms and R and R1 when taken together with the nitrogen atom to which they are attached represent the piperidino radical, R2 is hydrogen or methyl, Z is selected from the group consisting of oxygen, imino and methylene, Y is nitrogen or methylidene, or a pharma-ceutically acceptable acid addition salt thereof, which comprises a process selected from:
(a) whenever Z is methylene, reacting a 2,7-bis-basic ether of fluoren-9-one having the formula with diazomethane, and isolating the resulting product therefrom;
(b) whenever Z is imino and R2 is hydrogen, reacting a 2,7-bis-basic ether of fluoren-9-one having the formula with hydrazoic acid in the presence of a strong mineral acid, and isolating the resulting product therefrom; or (c) whenever Z is oxygen and R2 is hydrogen, reacting a 2,7-bis(.omega.-haloalkoxy)fluoren-9-one having tile formula with a peracid or with hydrogen peroxide in the presence of a strong acid catalyst to form a 3,8 bis(.omega.-haloalkoxy)6H-dibenzo[b,d]pyran-6-one, condensing said dibenzo[b,d]pyran-6-one with an amine having the formula and isolating the resulting product therefrom.
12. A bis-basic ether of 9-substituted phenanthrene or 10-oxa and 10-aza derivatives thereof having the formulae:
or wherein n is an integer of from 2 to 6, R and R1 are each selected from the group consisting of lower alkyl having from 1 to 6 carbon atoms and R and R1 when taken together with the nitrogen atom to which they are attached represent the piperidino radical, R2 is hydrogen or methyl, Z is selected from the group consisting of oxygen, imino and methylene, Y is nitrogen or methylidene, or a pharmaceuti-cally acceptable acid addition salt thereof, when prepared by the process of claim 11.
or wherein n is an integer of from 2 to 6, R and R1 are each selected from the group consisting of lower alkyl having from 1 to 6 carbon atoms and R and R1 when taken together with the nitrogen atom to which they are attached represent the piperidino radical, R2 is hydrogen or methyl, Z is selected from the group consisting of oxygen, imino and methylene, Y is nitrogen or methylidene, or a pharmaceuti-cally acceptable acid addition salt thereof, when prepared by the process of claim 11.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US31723772A | 1972-12-21 | 1972-12-21 |
Publications (1)
Publication Number | Publication Date |
---|---|
CA1042439A true CA1042439A (en) | 1978-11-14 |
Family
ID=23232741
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA184,541A Expired CA1042439A (en) | 1972-12-21 | 1973-10-29 | Bis-basic ethers of 9-substituted phenanthrene and 10-oxa and 10-aza derivatives thereof |
Country Status (8)
Country | Link |
---|---|
JP (1) | JPS4988852A (en) |
AU (1) | AU473526B2 (en) |
CA (1) | CA1042439A (en) |
DE (1) | DE2362577A1 (en) |
FR (1) | FR2211242B1 (en) |
GB (1) | GB1420377A (en) |
IL (1) | IL43674A (en) |
ZA (1) | ZA738207B (en) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20050171079A1 (en) * | 2004-02-04 | 2005-08-04 | Schrimpf Michael R. | Amino-substituted tricyclic derivatives and methods of use |
JP5745209B2 (en) * | 2005-12-22 | 2015-07-08 | メルク パテント ゲゼルシャフト ミット ベシュレンクテル ハフツングMerck Patent Gesellschaft mit beschraenkter Haftung | Benzochromene derivatives for use in liquid crystal media and as therapeutically active substances |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
IL33573A (en) * | 1968-12-30 | 1973-10-25 | Richardson Merrell Inc | Bis-basic ethers and thioethers of fluorenone,fluorenol and fluorene |
CA993795A (en) * | 1970-12-10 | 1976-07-27 | Richardson-Merrell (Canada) Ltd. | Antiviral compositions containing bis-basic ethers and thioethers of xanthene and xanthen-9-ones and methods of treating viruses therewith |
-
1973
- 1973-10-23 ZA ZA00738207A patent/ZA738207B/en unknown
- 1973-10-26 AU AU61883/73A patent/AU473526B2/en not_active Expired
- 1973-10-29 CA CA184,541A patent/CA1042439A/en not_active Expired
- 1973-11-21 IL IL43674A patent/IL43674A/en unknown
- 1973-12-17 GB GB5826673A patent/GB1420377A/en not_active Expired
- 1973-12-17 DE DE2362577A patent/DE2362577A1/en not_active Withdrawn
- 1973-12-19 FR FR7345518A patent/FR2211242B1/fr not_active Expired
- 1973-12-20 JP JP48141923A patent/JPS4988852A/ja active Pending
Also Published As
Publication number | Publication date |
---|---|
FR2211242B1 (en) | 1976-12-31 |
GB1420377A (en) | 1976-01-07 |
JPS4988852A (en) | 1974-08-24 |
AU473526B2 (en) | 1976-06-24 |
FR2211242A1 (en) | 1974-07-19 |
ZA738207B (en) | 1975-02-26 |
IL43674A (en) | 1977-02-28 |
IL43674A0 (en) | 1974-06-30 |
AU6188373A (en) | 1975-05-01 |
DE2362577A1 (en) | 1974-06-27 |
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