BRPI0515418A - método para a produção de dna de plasmìdio super-enrolado fechado covalentemente e composição de matéria - Google Patents

método para a produção de dna de plasmìdio super-enrolado fechado covalentemente e composição de matéria

Info

Publication number
BRPI0515418A
BRPI0515418A BRPI0515418-9A BRPI0515418A BRPI0515418A BR PI0515418 A BRPI0515418 A BR PI0515418A BR PI0515418 A BRPI0515418 A BR PI0515418A BR PI0515418 A BRPI0515418 A BR PI0515418A
Authority
BR
Brazil
Prior art keywords
matter
composition
production
plasmid dna
covalently closed
Prior art date
Application number
BRPI0515418-9A
Other languages
English (en)
Inventor
Clague P Hodgson
James A Williams
Original Assignee
Nature Technology Corp
Clague P Hodgson
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nature Technology Corp, Clague P Hodgson filed Critical Nature Technology Corp
Publication of BRPI0515418A publication Critical patent/BRPI0515418A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

MéTODO PARA A PRODUçãO DE DNA DE PLASMìDIO SUPER-ENROLADO FECHADO COVALENTEMENTE E COMPOSIçãO DE MATéRIA. Um método geral e cepas de bactérias são descritos, por meio do qual é possível purificar drasticamente o DNA de plasmídio com respeito ao DNA genómico, e o RNA. Em uma realização preterida, a lise e a remoçãoda nuclease de ácidos nucléicos hospedeiros é um componente integral do processo de fermentação/colheita, e dessa maneira é obtido um rendimento maior e uma pureza com a purificação a jusante simplificada e correntes residuais reduzidas, reduzindo desse modo os custos de produção.
BRPI0515418-9A 2004-08-16 2005-08-15 método para a produção de dna de plasmìdio super-enrolado fechado covalentemente e composição de matéria BRPI0515418A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US60207404P 2004-08-16 2004-08-16
PCT/US2005/028870 WO2006026125A2 (en) 2004-08-16 2005-08-15 Improved strains of e. coli for plasmid dna production

Publications (1)

Publication Number Publication Date
BRPI0515418A true BRPI0515418A (pt) 2008-07-22

Family

ID=36000523

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0515418-9A BRPI0515418A (pt) 2004-08-16 2005-08-15 método para a produção de dna de plasmìdio super-enrolado fechado covalentemente e composição de matéria

Country Status (14)

Country Link
US (1) US8748168B2 (pt)
EP (1) EP1781801A4 (pt)
JP (1) JP2008509704A (pt)
KR (1) KR20070053268A (pt)
CN (1) CN101035905A (pt)
AU (1) AU2005280356B2 (pt)
BR (1) BRPI0515418A (pt)
CA (1) CA2577419A1 (pt)
EA (1) EA010864B1 (pt)
IL (1) IL181319A0 (pt)
MX (1) MX2007001847A (pt)
NZ (1) NZ553658A (pt)
WO (1) WO2006026125A2 (pt)
ZA (1) ZA200701265B (pt)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8227227B2 (en) * 2006-11-30 2012-07-24 Novozymes A/S DNase expression in recombinant host cells
EP2160459A2 (en) 2007-05-23 2010-03-10 Nature Technology Corp. Improved e. coli plasmid dna production
CA2721153C (en) * 2008-04-10 2018-10-02 Abraxis Bioscience, Llc Compositions of hydrophobic taxane derivatives and uses thereof
BR112019002679A2 (pt) * 2016-08-08 2019-05-14 Karius, Inc. redução de sinal de ácidos nucleicos contaminantes
CN108220317B (zh) * 2017-12-11 2021-04-16 北京大学 一种重组表达质粒及其制备方法、用途
GB201814590D0 (en) * 2018-09-07 2018-10-24 Oxford Biomedica Ltd Viral vector production system

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ZA842997B (en) 1983-04-21 1984-11-28 Australia Gentech Ltd Preparation of plasmid dna and products thereof
WO1992013963A1 (en) 1991-01-30 1992-08-20 Hyman Edward D Method for preparation of closed circular dna
FR2728264B1 (fr) 1994-12-16 1997-01-31 Rhone Poulenc Rorer Sa Purification d'adn par formation de triple helice avec un oligonucleotide immobilise
US6455287B1 (en) 1995-02-23 2002-09-24 Wyeth Mechanical disruption of bacterial cells for plasmid recovery
US5591064A (en) 1995-06-30 1997-01-07 Church & Dwight Co., Inc. Blasting apparatus and method for blast cleaning a solid surface
FR2746412B1 (fr) 1996-03-21 1998-06-12 Rhone Poulenc Rorer Sa Purification d'adn plasmidique de qualite pharmaceutique
US7026468B2 (en) 1996-07-19 2006-04-11 Valentis, Inc. Process and equipment for plasmid purification
US6011148A (en) 1996-08-01 2000-01-04 Megabios Corporation Methods for purifying nucleic acids
US6214586B1 (en) 1997-12-08 2001-04-10 Genzyme Corporation Method for purifying plasmid DNA and plasmid DNA substantially free of genomic DNA
JP4298165B2 (ja) 1998-03-30 2009-07-15 メタボリックス，インコーポレイテッド 生体適合材料の製造のための微生物株およびプロセス
EP0992583B1 (en) * 1998-09-14 2003-03-26 QIAGEN GmbH Novel method for purifying covalently closed circular DNA
EP0987327A1 (en) * 1998-09-14 2000-03-22 QIAGEN GmbH Novel method for purifying covalently closed circular DNA
DK1323820T3 (da) 1998-10-28 2009-03-16 Genentech Inc Fremgangsmåde til faciliteret isolering af heterologe proteiner fra bakterieceller
WO2000028048A1 (en) 1998-11-09 2000-05-18 Genecare Development Aps Novel plasmids for use in medicine and method of producing same
GB9924973D0 (en) 1999-10-21 1999-12-22 Cobra Therapeutics Ltd Preparation of cellular components that are substantially free of chromosomal DNA
US20020012990A1 (en) 1999-12-22 2002-01-31 Lander Russel Jackson Process for the scaleable purification of plasmid DNA
CA2414775A1 (en) 2000-05-03 2001-11-08 Expressive Constructs, Inc. A method and device for improving protein stability and solubility
US20020197637A1 (en) 2001-06-02 2002-12-26 Willson Richard C. Process and compositions for protection of nucleic acids
US20030092885A1 (en) 2001-07-31 2003-05-15 President And Fellows Of Harvard College Solubility and stability enhancement tag for structural and ligand binding studies of proteins
GB0127809D0 (en) 2001-11-20 2002-01-09 Dna Res Innovations Ltd Purification of nucleic acid
WO2003044051A1 (en) 2001-11-20 2003-05-30 Atgen Co., Ltd. Novel peptides conferring environmental stress resistance and fusion proteins including said peptides

Also Published As

Publication number Publication date
MX2007001847A (es) 2007-04-13
EA200700415A1 (ru) 2007-10-26
ZA200701265B (en) 2008-04-30
WO2006026125A2 (en) 2006-03-09
JP2008509704A (ja) 2008-04-03
US8748168B2 (en) 2014-06-10
AU2005280356A1 (en) 2006-03-09
WO2006026125A3 (en) 2006-07-06
AU2005280356B2 (en) 2008-09-25
US20070249042A1 (en) 2007-10-25
NZ553658A (en) 2009-11-27
CN101035905A (zh) 2007-09-12
CA2577419A1 (en) 2006-03-09
EA010864B1 (ru) 2008-12-30
IL181319A0 (en) 2007-07-04
EP1781801A4 (en) 2008-11-12
EP1781801A2 (en) 2007-05-09
KR20070053268A (ko) 2007-05-23

Similar Documents

Publication Publication Date Title
Zhang et al. Multiplex gene regulation by CRISPR-ddCpf1
KR102623312B1 (ko) Ruvc 도메인이 존재하는 효소
JP7050866B2 (ja) オリゴヌクレオチドの産生のための新規プロセス
BRPI0515418A (pt) método para a produção de dna de plasmìdio super-enrolado fechado covalentemente e composição de matéria
BRPI0814781A8 (pt) ''proteína, sequência de ácido nucleico, método para a produção de ácido itacônico, vetor, célula hospedeira, e, uso de um ácido cis-aconítico descarboxilase''
BRPI0507077A (pt) processo para a preparação de l-aminoácidos usando cepas da famìlia enterobacteriaceae
EP2239327A3 (en) Method for in vitro recombination
WO2007148091B1 (en) Dna molecules and methods
WO2008020333A3 (en) Nucleic acid encoding n-methylputrescine oxidase and uses thereof
MX2011007693A (es) Metodos para amplificar acidos nucleicos del virus de hepatitis c.
TW200708734A (en) Microchip electrophoresis method and device
Pereira et al. Advances in time course extracellular production of human pre-miR-29b from Rhodovulum sulfidophilum
WO2023132885A1 (en) Methods of purifying dna for gene synthesis
CN113943764B (zh) 一种制备双链rna的方法
EP4127156A1 (en) Class ii, type ii crispr systems
EP4028515A1 (en) Novel class 2 type ii and type v crispr-cas rna-guided endonucleases
KR20240055073A (ko) 클래스 ii, v형 crispr 시스템
PL1608752T3 (pl) Sposób izolacji kwasów nukleinowych
AU2022396533A1 (en) Endonuclease systems
Katayama et al. Editing of DNA methylation using CRISPR/Cas9 and a ssDNA template in human cells
EP4240853A2 (en) Novel class 2 crispr-cas rna-guided endonucleases
Liu et al. Allele-specific genome editing of imprinting genes by preferentially targeting non-methylated loci using Staphylococcus aureus Cas9 (SaCas9)
EP4127155A1 (en) Class ii, type ii crispr systems
US9695417B2 (en) Method for large-scale synthesis of long-chain nucleic acid molecule
CN113728097A (zh) 具有ruvc结构域的酶

Legal Events

Date Code Title Description
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE A 6A ANUIDADE.

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 2160 DE 29/05/2012.