BRPI0416807A - sistemas de expressão aperfeiçoados com secreção de sistema sec - Google Patents
sistemas de expressão aperfeiçoados com secreção de sistema secInfo
- Publication number
- BRPI0416807A BRPI0416807A BRPI0416807-0A BRPI0416807A BRPI0416807A BR PI0416807 A BRPI0416807 A BR PI0416807A BR PI0416807 A BRPI0416807 A BR PI0416807A BR PI0416807 A BRPI0416807 A BR PI0416807A
- Authority
- BR
- Brazil
- Prior art keywords
- sec
- secretion
- expression systems
- systems
- sec system
- Prior art date
Links
- 230000028327 secretion Effects 0.000 title abstract 5
- 230000014509 gene expression Effects 0.000 title abstract 2
- 108010076504 Protein Sorting Signals Proteins 0.000 abstract 2
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 abstract 2
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 abstract 2
- 241000589540 Pseudomonas fluorescens Species 0.000 abstract 1
- 102000037865 fusion proteins Human genes 0.000 abstract 1
- 108020001507 fusion proteins Proteins 0.000 abstract 1
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 1
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 1
- 230000009465 prokaryotic expression Effects 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/21—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Pseudomonadaceae (F)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormone [GH], i.e. somatotropin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/16—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from plants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/78—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Pseudomonas
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
- C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
- C07K2317/622—Single chain antibody (scFv)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Gastroenterology & Hepatology (AREA)
- Immunology (AREA)
- Endocrinology (AREA)
- Botany (AREA)
- Toxicology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Abstract
"SISTEMAS DE EXPRESSãO APERFEIçOADOS COM SECREçãO DE SISTEMA SEC". Métodos e sistemas de expressão procarióticos aperfeiçoados para produção de proteínas recombinantes usando peptídeos de sinal de secreção de sistema Sec são descritos usando sistemas de secreção de Sec de Pseudomonas fluorescens. Peptídeos de sinal de secreção de sistema Sec novos específicos são descritos, como o são proteínas de fusão e seqüências de codificação para secreção aperfeiçoada de proteínas e peptídeos recombinantes.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US52412403P | 2003-11-21 | 2003-11-21 | |
PCT/US2004/039316 WO2005089093A2 (en) | 2003-11-21 | 2004-11-22 | Improved expression systems with sec-system secretion |
Publications (1)
Publication Number | Publication Date |
---|---|
BRPI0416807A true BRPI0416807A (pt) | 2007-01-09 |
Family
ID=34994140
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BRPI0416807-0A BRPI0416807A (pt) | 2003-11-21 | 2004-11-22 | sistemas de expressão aperfeiçoados com secreção de sistema sec |
Country Status (11)
Country | Link |
---|---|
US (1) | US7985564B2 (pt) |
EP (4) | EP2336153B1 (pt) |
JP (1) | JP5028551B2 (pt) |
KR (1) | KR101265343B1 (pt) |
CN (1) | CN1882605B (pt) |
AU (1) | AU2004317306B2 (pt) |
BR (1) | BRPI0416807A (pt) |
CA (1) | CA2546157C (pt) |
MX (1) | MXPA06005705A (pt) |
PL (2) | PL2336153T3 (pt) |
WO (1) | WO2005089093A2 (pt) |
Families Citing this family (33)
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US9453251B2 (en) | 2002-10-08 | 2016-09-27 | Pfenex Inc. | Expression of mammalian proteins in Pseudomonas fluorescens |
JP5028551B2 (ja) | 2003-11-21 | 2012-09-19 | フェネックス インコーポレイテッド | Sec系分泌によって改良された発現系 |
WO2006014899A2 (en) | 2004-07-26 | 2006-02-09 | Dow Global Technologies Inc. | Process for improved protein expression by strain engineering |
JP5130662B2 (ja) * | 2006-06-07 | 2013-01-30 | Jnc株式会社 | タンパク質の可溶性タンパク質としての製造方法 |
AU2008210538B2 (en) * | 2007-01-31 | 2013-06-06 | Pelican Technology Holdings, Inc. | Bacterial leader sequences for increased expression |
US9394571B2 (en) | 2007-04-27 | 2016-07-19 | Pfenex Inc. | Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins |
US9580719B2 (en) | 2007-04-27 | 2017-02-28 | Pfenex, Inc. | Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins |
WO2009076196A1 (en) | 2007-12-07 | 2009-06-18 | Dow Global Technologies Inc. | High copy number self-replicating plasmids in pseudomonas |
WO2010008764A1 (en) * | 2008-06-23 | 2010-01-21 | Dow Global Technologies Inc. | Pseudomonas fluorescens strains for production of extracellular recombinant protein |
UA106885C2 (uk) | 2009-04-17 | 2014-10-27 | Дау Агросайансіз Елелсі | Dig-3 інсектицидні cry-токсини |
JP2013514769A (ja) | 2009-12-16 | 2013-05-02 | ダウ アグロサイエンシィズ エルエルシー | 害虫抵抗性管理のためのCRY1CaおよびCRY1Faタンパク質の併用 |
ES2639398T3 (es) | 2010-03-04 | 2017-10-26 | Pfenex Inc. | Método para producir proteína de interferón recombinante soluble sin desnaturalización |
EP2553102B1 (en) | 2010-03-30 | 2015-12-09 | Pfenex Inc. | High level expression of recombinant toxin proteins |
CA2794740C (en) | 2010-04-01 | 2019-12-31 | Pfenex Inc. | Methods for g-csf production in a pseudomonas host cell |
RU2582249C2 (ru) | 2010-04-23 | 2016-04-20 | ДАУ АГРОСАЙЕНСИЗ ЭлЭлСи | КОМБИНАЦИИ, ВКЛЮЧАЮЩИЕ БЕЛКИ Cry34Ab/35Ab И Cry3Aa, ДЛЯ ПРЕДОТВРАЩЕНИЯ РАЗВИТИЯ УСТОЙЧИВОСТИ У КУКУРУЗНЫХ КОРНЕВЫХ ЖУКОВ (Diabrotica spp.) |
US9234208B1 (en) | 2010-05-10 | 2016-01-12 | Dow Agrosciences Llc | DIG-13 insecticidal cry toxins |
AR084293A1 (es) | 2010-12-16 | 2013-05-08 | Dow Agrosciences Llc | Cry1FA RADIOMARCADA, BIOLOGICAMENTE ACTIVA Y METODOS DE ENSAYOS DE UNION AL RECEPTOR |
JP6362543B2 (ja) | 2012-02-23 | 2018-07-25 | ジュノ セラピューティクス ゲーエムベーハー | 細胞および他の複雑な生物学的材料のクロマトグラフィーによる単離 |
EP2841590A4 (en) * | 2012-04-27 | 2016-03-23 | Pronutria Inc | NUCLEIC ACIDS, CELLS AND METHODS FOR PRODUCING SECRETED PROTEINS |
US9169304B2 (en) | 2012-05-01 | 2015-10-27 | Pfenex Inc. | Process for purifying recombinant Plasmodium falciparum circumsporozoite protein |
CN103254277B (zh) * | 2013-05-03 | 2017-02-08 | 南京工业大学 | 强分泌性信号肽增强小肽模序及其应用 |
AR099800A1 (es) | 2014-03-21 | 2016-08-17 | Agrigenetics Inc | Cry1d para controlar el gusano de la mazorca del maíz |
NZ726117A (en) | 2014-06-20 | 2017-10-27 | Dow Agrosciences Llc | Vegetative insecticidal proteins useful for control of insect pests |
EP3227455B1 (en) | 2014-12-01 | 2023-07-12 | Pfenex Inc. | Fusion partners for peptide production |
MX2017008413A (es) | 2014-12-30 | 2017-10-19 | Dow Agrosciences Llc | Toxinas cry1ca modificadas utiles para el control de plagas de insectos. |
MA45489A (fr) | 2015-10-22 | 2018-08-29 | Juno Therapeutics Gmbh | Procédés de culture de cellules, kits et appareil associés |
CA3038972A1 (en) | 2016-09-30 | 2018-04-05 | Dow Agrosciences Llc | Binary insecticidal cry toxins |
AR111625A1 (es) | 2017-04-27 | 2019-07-31 | Juno Therapeutics Gmbh | Reactivos de partículas oligoméricas y métodos de uso de los mismos |
CA3077393A1 (en) * | 2017-10-27 | 2019-05-02 | Pfenex Inc. | Bacterial leader sequences for periplasmic protein expression |
CN111278852B (zh) | 2017-10-27 | 2024-07-16 | 菲尼克斯公司 | 重组欧氏杆菌天冬酰胺酶的生产方法 |
CN110819647A (zh) * | 2018-08-07 | 2020-02-21 | 康码(上海)生物科技有限公司 | 信号肽相关序列及其在蛋白质合成中的应用 |
CN112812176B (zh) * | 2021-01-13 | 2022-10-11 | 江南大学 | 一种通过低盐絮凝法从螺旋藻中提取藻蓝蛋白的方法 |
WO2022211829A1 (en) | 2021-03-30 | 2022-10-06 | Jazz Pharmaceuticals Ireland Ltd. | Dosing of recombinant l-asparaginase |
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-
2004
- 2004-11-22 JP JP2006541651A patent/JP5028551B2/ja active Active
- 2004-11-22 WO PCT/US2004/039316 patent/WO2005089093A2/en not_active Application Discontinuation
- 2004-11-22 MX MXPA06005705A patent/MXPA06005705A/es active IP Right Grant
- 2004-11-22 EP EP10179651.4A patent/EP2336153B1/en active Active
- 2004-11-22 US US10/996,007 patent/US7985564B2/en active Active
- 2004-11-22 PL PL10179651T patent/PL2336153T3/pl unknown
- 2004-11-22 AU AU2004317306A patent/AU2004317306B2/en active Active
- 2004-11-22 CN CN200480034455XA patent/CN1882605B/zh not_active Expired - Fee Related
- 2004-11-22 BR BRPI0416807-0A patent/BRPI0416807A/pt not_active IP Right Cessation
- 2004-11-22 EP EP10179657A patent/EP2314602A1/en not_active Withdrawn
- 2004-11-22 PL PL10179615.9T patent/PL2327718T3/pl unknown
- 2004-11-22 CA CA2546157A patent/CA2546157C/en active Active
- 2004-11-22 EP EP10179615.9A patent/EP2327718B1/en active Active
- 2004-11-22 EP EP04817876A patent/EP1687324B1/en active Active
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2006
- 2006-05-19 KR KR1020067009779A patent/KR101265343B1/ko active IP Right Grant
Also Published As
Publication number | Publication date |
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WO2005089093A3 (en) | 2006-03-02 |
MXPA06005705A (es) | 2006-08-23 |
PL2327718T3 (pl) | 2016-10-31 |
EP1687324B1 (en) | 2012-08-22 |
PL2336153T3 (pl) | 2016-08-31 |
JP5028551B2 (ja) | 2012-09-19 |
KR101265343B1 (ko) | 2013-05-20 |
CA2546157A1 (en) | 2005-09-29 |
EP2314602A1 (en) | 2011-04-27 |
AU2004317306B2 (en) | 2010-09-02 |
EP2327718B1 (en) | 2016-03-23 |
AU2004317306A1 (en) | 2005-09-29 |
JP2007512028A (ja) | 2007-05-17 |
EP1687324A2 (en) | 2006-08-09 |
EP2336153B1 (en) | 2016-03-30 |
EP2336153A1 (en) | 2011-06-22 |
US7985564B2 (en) | 2011-07-26 |
KR20060123173A (ko) | 2006-12-01 |
EP2327718A1 (en) | 2011-06-01 |
CA2546157C (en) | 2014-07-22 |
CN1882605A (zh) | 2006-12-20 |
EP1687324A4 (en) | 2007-08-22 |
WO2005089093A2 (en) | 2005-09-29 |
US20060008877A1 (en) | 2006-01-12 |
CN1882605B (zh) | 2012-07-11 |
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