BR9914773A - Set of generic elements, method for the production of single-stranded DNA, mrna transcription, nucleic acid construction, ssdna transcription, vector, vector system, host cell, set for the production of a single-stranded nucleic acid sequence, method for in vivo or in vitro production of a single-stranded nucleic acid sequence, transcription of single-stranded cdna, inhibitory nucleic acid, heteroduplex molecule, and pharmaceutical composition - Google Patents

Set of generic elements, method for the production of single-stranded DNA, mrna transcription, nucleic acid construction, ssdna transcription, vector, vector system, host cell, set for the production of a single-stranded nucleic acid sequence, method for in vivo or in vitro production of a single-stranded nucleic acid sequence, transcription of single-stranded cdna, inhibitory nucleic acid, heteroduplex molecule, and pharmaceutical composition

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Publication number
BR9914773A
BR9914773A BR9914773-4A BR9914773A BR9914773A BR 9914773 A BR9914773 A BR 9914773A BR 9914773 A BR9914773 A BR 9914773A BR 9914773 A BR9914773 A BR 9914773A
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BR
Brazil
Prior art keywords
nucleic acid
stranded
cdna
transcription
production
Prior art date
Application number
BR9914773-4A
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Portuguese (pt)
Inventor
Charles A Conrad
Original Assignee
Ingene Inc
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Publication date
Application filed by Ingene Inc filed Critical Ingene Inc
Publication of BR9914773A publication Critical patent/BR9914773A/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C12N2310/111—Antisense spanning the whole gene, or a large part of it
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

"CONJUNTO DE ELEMENTOS GENéTICOS, MéTODO PARA A PRODUçãO DE DNA DE CORDãO úNICO, TRANSCRIçãO DE mRNA, CONSTRUçãO DE áCIDO NUCLéICO, TRANSCRIçãO DE ssDNA, VETOR, SISTEMA VETOR, CéLULA HOSPEDEIRA, CONJUNTO PARA A PRODUçãO DE UMA SEQuêNCIA DE áCIDO NUCLéICO DE CORDãO úNICO, MéTODO PARA A PRODUçãO IN VIVO OU IN VITRO DE UMA SEQuêNCIA DE áCIDO NUCLéICO DE CORDãO úNICO, TRANSCRIçãO DE CDNA DE CORDãO úNICO, áCIDO NUCLéICO INIBIDOR, MOLéCULA HETERODUPLEX E COMPOSIçãO FARMACêUTICA". Métodos e composições para a produção de cDNA de um único filamento (ss-cDNA) em células eucarióticas, especificamente um cassete de DNA que produz s-cDNA in vivo O cassete contém o gene de codificação de transcriptase/Rnase H reversa do vírus de leucemia de murinos de Moloney, um gene de endonuclease de restrição de bactérias, e uma seq³ência de interesse que produz um modelo de RNA a partir do qual a transcriptase reversa sintetiza o ss-cDNA de uma seq³ência específica. O ss-cDNA é então modificado para remover todas as seq³ências de vetores de flanqueamento ao tirar vantagem da estrutura de "haste-laço" do ss-cDNA, que se forma como resultado da inclusão de uma repetição em tandem invertida que permite que o ss-cDNA de dobre de volta sobre si mesmo, formando uma haste de DNA de filamento duplo, na seq³ência de interesse. A haste de filamento duplo contém um ou mais elementos genéticos funcionais, tais como um sítio de reconhecimento de endonuclease de restrição e o laço, que continua como ss-cDNA, compreende qualquer seq³ência de nucleotídeos desejada. Esse desenho permite que a haste de filamento duplo do intermediário de haste-laço a ser clivada pela(s) endonuclease(s) de restrição correspondente(s) desejada(s) específica(s) para o sítio na haste e na porção do laço, ou seq³ência de interesse, seja então liberada como um pedaço de DNA de um único filamento linearizado. Esse pedaço de ss-DNA liberado (ou clivado) contém informações mínimas, caso haja alguma, sobre a seq³ência tanto a montante da posição 51 quanto a jusante da posição 3' em relação à porção da haste de filamento duplo anterior que contém o sítio de corte de endonuclease de restrição. As transfecções in vivo utilizando o sistema de vetores de DNA aqui descrito demonstram o uso desse sistema para produzir ss-DNA em células hospedeiras."SET OF GENETIC ELEMENTS, METHOD FOR THE PRODUCTION OF SINGLE CORD DNA, MRNA TRANSCRIPTION, NUCLEIC ACID CONSTRUCTION, SDSNA TRANSCRIPTION, VECTOR, VECTOR SYSTEM, HOSPITAL CELL, NUCLEUM CUTTING UNIT , METHOD FOR THE IN VIVO OR IN VITRO PRODUCTION OF A SINGLE CORDANIC NUCLEIC ACID, TRANSCRIPTION OF SINGLE CORDANIC CDNA, INHIBITOR NUCLEIC ACID, HETERODUPLEX MOLECULE AND PHARMACEUTICAL COMPOSITION ". Methods and compositions for the production of single-strand cDNA (ss-cDNA) in eukaryotic cells, specifically a DNA cassette that produces s-cDNA in vivo The cassette contains the leukemia virus reverse transcriptase / R Hase encoding gene of Moloney murines, a bacterial restriction endonuclease gene, and a sequence of interest that produces an RNA model from which reverse transcriptase synthesizes the ss-cDNA of a specific sequence. The ss-cDNA is then modified to remove all flanking vector sequences by taking advantage of the ss-cDNA "rod-loop" structure, which is formed as a result of including an inverted tandem repeat that allows the ss -cDNA fold back on itself, forming a double-stranded DNA strand, in the sequence of interest. The double stranded rod contains one or more functional genetic elements, such as a restriction endonuclease recognition site and the loop, which continues as ss-cDNA, comprises any desired nucleotide sequence. This design allows the double-stranded rod of the rod-loop intermediate to be cleaved by the desired corresponding restriction endonuclease (s) specific to the site on the rod and portion of the loop , or sequence of interest, is then released as a piece of DNA from a single linearized strand. This piece of ss-DNA released (or cleaved) contains minimal information, if any, about the sequence both upstream of position 51 and downstream of position 3 'in relation to the portion of the anterior double-stranded rod containing the site of restriction endonuclease cut. In vivo transfections using the DNA vector system described here demonstrate the use of this system to produce ss-DNA in host cells.

BR9914773-4A 1998-10-09 1999-10-12 Set of generic elements, method for the production of single-stranded DNA, mrna transcription, nucleic acid construction, ssdna transcription, vector, vector system, host cell, set for the production of a single-stranded nucleic acid sequence, method for in vivo or in vitro production of a single-stranded nucleic acid sequence, transcription of single-stranded cdna, inhibitory nucleic acid, heteroduplex molecule, and pharmaceutical composition BR9914773A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US16979398A 1998-10-09 1998-10-09
US39778299A 1999-09-16 1999-09-16
PCT/US1999/023936 WO2000022114A1 (en) 1998-10-09 1999-10-12 PRODUCTION OF ssDNA $i(IN VIVO)

Publications (1)

Publication Number Publication Date
BR9914773A true BR9914773A (en) 2002-02-05

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BR9914773-4A BR9914773A (en) 1998-10-09 1999-10-12 Set of generic elements, method for the production of single-stranded DNA, mrna transcription, nucleic acid construction, ssdna transcription, vector, vector system, host cell, set for the production of a single-stranded nucleic acid sequence, method for in vivo or in vitro production of a single-stranded nucleic acid sequence, transcription of single-stranded cdna, inhibitory nucleic acid, heteroduplex molecule, and pharmaceutical composition

Country Status (5)

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AU (1) AU6298899A (en)
BR (1) BR9914773A (en)
CA (1) CA2345936A1 (en)
MX (1) MXPA01003643A (en)
WO (1) WO2000022114A1 (en)

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