BR9811542A - Identificação de linhas de células humanas para a produção de proteìnas humanas mediante ativação de genes endógena - Google Patents

Identificação de linhas de células humanas para a produção de proteìnas humanas mediante ativação de genes endógena

Info

Publication number
BR9811542A
BR9811542A BR9811542-1A BR9811542A BR9811542A BR 9811542 A BR9811542 A BR 9811542A BR 9811542 A BR9811542 A BR 9811542A BR 9811542 A BR9811542 A BR 9811542A
Authority
BR
Brazil
Prior art keywords
human
identification
production
human proteins
cell lines
Prior art date
Application number
BR9811542-1A
Other languages
English (en)
Inventor
Michael Brandt
Reinhard Franze
Ulrich Pessara
Original Assignee
Roche Diagnostics Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27238256&utm_source=***_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=BR9811542(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from US09/113,692 external-priority patent/US6548296B1/en
Application filed by Roche Diagnostics Gmbh filed Critical Roche Diagnostics Gmbh
Publication of BR9811542A publication Critical patent/BR9811542A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/505—Erythropoietin [EPO]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

Patente de Invenção:<B>"IDENTIFICAçãO DE LINHAS DE CéLULAS HUMANAS PARA A PRODUçãO DE PROTEìNAS HUMANAS MEDIANTE ATIVAçãO DE GENES ENDóGENA"<D>. A invenção refere-se a processos para a escolha de células humanas para a preparação de proteínas humanas mediante ativação de gene endógena, para produzir proteínas humanas em rendimentos económicos e em uma forma, que é adequada para a preparação de uma preparado farmacêutico. Além disso, a invenção refere-se a um processo para a preparação de proteínas humanas em uma linha de células identificadas desta maneira.
BR9811542-1A 1997-07-23 1998-07-22 Identificação de linhas de células humanas para a produção de proteìnas humanas mediante ativação de genes endógena BR9811542A (pt)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
EP97112640 1997-07-23
EP97121073 1997-12-01
US09/113,692 US6548296B1 (en) 1997-07-23 1998-07-10 Methods for identifying human cell lines useful for endogenous gene activation, isolated human lines identified thereby, and uses thereof
PCT/EP1998/004584 WO1999005267A1 (de) 1997-07-23 1998-07-22 Identifizierung humaner zellinien zur produktion humaner proteine durch endogene genaktivierung

Publications (1)

Publication Number Publication Date
BR9811542A true BR9811542A (pt) 2000-08-22

Family

ID=27238256

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9811542-1A BR9811542A (pt) 1997-07-23 1998-07-22 Identificação de linhas de células humanas para a produção de proteìnas humanas mediante ativação de genes endógena

Country Status (14)

Country Link
US (1) US6395484B1 (pt)
EP (1) EP1000154B1 (pt)
JP (1) JP2001511342A (pt)
CN (1) CN1150319C (pt)
AT (1) ATE354654T1 (pt)
AU (1) AU737605B2 (pt)
BR (1) BR9811542A (pt)
CA (1) CA2298412C (pt)
DE (1) DE59813913D1 (pt)
DK (1) DK1000154T3 (pt)
ES (1) ES2281133T3 (pt)
TR (1) TR200000140T2 (pt)
TW (1) TWI224140B (pt)
WO (1) WO1999005267A1 (pt)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT1000154E (pt) * 1997-07-23 2007-03-30 Roche Diagnostics Gmbh Identificação de linhas celulares humanas para a produção de proteínas humanas por activação endógena de genes
WO2003054172A2 (en) * 2002-01-17 2003-07-03 Lonza Biologics Plc. Glutamine-auxothrophic human cells capable of producing proteins and capable of growing in a glutamine-free medium
GB0200977D0 (en) * 2002-01-17 2002-03-06 Lonza Biologics Plc Glutamine-auxotrophic human cells capable of producing proteins and capable of growing in a glutamine-free medium
GB0208041D0 (en) * 2002-04-08 2002-05-22 Lonza Biologics Plc Method of culturing animal cells
US11311574B2 (en) 2003-08-08 2022-04-26 Sangamo Therapeutics, Inc. Methods and compositions for targeted cleavage and recombination
US7888121B2 (en) 2003-08-08 2011-02-15 Sangamo Biosciences, Inc. Methods and compositions for targeted cleavage and recombination
US8409861B2 (en) 2003-08-08 2013-04-02 Sangamo Biosciences, Inc. Targeted deletion of cellular DNA sequences
JP4849408B2 (ja) 2004-03-01 2012-01-11 財団法人北九州産業学術推進機構 タンパク質産生新規ヒト細胞株、新規ヒト細胞株の選択方法、新規ヒト細胞株の使用、それらからのタンパク質産生方法及び精製方法、及び新規ヒト細胞株を利用した薬学組成物
DE102012009849A1 (de) 2012-05-21 2013-11-21 Hartwig Schwieger Schwingungsstabilisierte Windenergieanlage-Zug-Druck-Knoten
JP6652334B2 (ja) 2014-05-31 2020-02-19 Ｊｃｒファーマ株式会社 ウリジンとｎ−アセチル−ｄ−マンノサミンとを含有する培地
DE112019003498T5 (de) * 2018-07-09 2021-04-08 NanoCav, LLC Mikrodurchflusselektroporationsgeräte und verfahren zur zelltransfektion

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL77081A (en) 1984-12-04 1999-10-28 Genetics Inst AND sequence encoding human erythropoietin, a process for its preparation and a pharmacological preparation of human erythropoietin
JPS62171696A (ja) 1986-01-23 1987-07-28 Sumitomo Chem Co Ltd ヒトエリスロポエチンの製造方法
US4954437A (en) 1986-09-15 1990-09-04 Integrated Genetics, Inc. Cell encoding recombinant human erythropoietin
JP2879906B2 (ja) * 1989-09-14 1999-04-05 鐘紡株式会社 プロコラゲナーゼの製造方法
SG88714A1 (en) * 1989-11-06 2002-05-21 Cell Genesys Inc Production of proteins using homologous recombination
US5328844A (en) * 1990-05-09 1994-07-12 University Of Colorado Foundation, Inc. Culture media for mammalian cells
US5641670A (en) * 1991-11-05 1997-06-24 Transkaryotic Therapies, Inc. Protein production and protein delivery
US6270989B1 (en) * 1991-11-05 2001-08-07 Transkaryotic Therapies, Inc. Protein production and delivery
PT101031B (pt) * 1991-11-05 2002-07-31 Transkaryotic Therapies Inc Processo para o fornecimento de proteinas por terapia genetica
TW402639B (en) * 1992-12-03 2000-08-21 Transkaryotic Therapies Inc Protein production and protein delivery
US5449015A (en) * 1994-05-12 1995-09-12 Petkovic; Tony Shock-activated shut-off valve

Also Published As

Publication number Publication date
US6395484B1 (en) 2002-05-28
AU737605B2 (en) 2001-08-23
ATE354654T1 (de) 2007-03-15
AU9067498A (en) 1999-02-16
ES2281133T3 (es) 2007-09-16
TWI224140B (en) 2004-11-21
CA2298412A1 (en) 1999-02-04
CN1265144A (zh) 2000-08-30
EP1000154A1 (de) 2000-05-17
EP1000154B1 (de) 2007-02-21
CA2298412C (en) 2010-10-19
WO1999005267A1 (de) 1999-02-04
DK1000154T3 (da) 2007-06-18
DE59813913D1 (de) 2007-04-05
TR200000140T2 (tr) 2000-05-22
CN1150319C (zh) 2004-05-19
JP2001511342A (ja) 2001-08-14

Similar Documents

Publication Publication Date Title
ATE219380T1 (de) Vehikel zum einbringen von nukleinsäuren in zellen
BR9809006A (pt) Composição, processo para produzir a mesma, processos de liberação de uma proteìna a uma célula de um indivìduo, de liberação de uma proteìna, polipeptìdeo, peptìdeo e/ou medicamentos não proteináceos/composto terapêutico a uma célula de um indivìduo, para induzir uma resposta imune em um indivìduo, para liberar uma molécula de ácido nucleico a uma célula de um indivìduo,e, composição farmacêutica
NO981944L (no) Lipopolyaminer som transfeksjonsmidler samt farmas÷ytisk anvendelse derav
BR9811542A (pt) Identificação de linhas de células humanas para a produção de proteìnas humanas mediante ativação de genes endógena
BR9803807A (pt) Processo para produção de protéina.
BR0007663A (pt) Derivados de acila para uso no tratamento de doenças relacionadas com o vla-4
BR0108545A (pt) Estimulação e concentração simultâneas das células
BR0207325A (pt) Métodos terapêuticos para doenças associadas com expressão diminuìda de gene de aop-1 ou aop-1
BR0011500A (pt) Hialuronidase de hirudinaria manillensis, isolamento, purificação e método recombinante de produção
DE60140218D1 (de) Expressionsvektor für tierische zellen
MX9803004A (es) Nuevos derivados de aminoacidos, procedimiento para su preparacion y composiciones farmaceuticas que contienen estos compuestos.
ATE347911T1 (de) Zusammensetzungen für die zufuhr von genen an antigen-präsentierende zellen der haut
DK0910647T3 (da) Human DNase i hyperaktive varianter
CY1105416T1 (el) Μεθοδος παρασκευης πολυαλκυλενιμινων που φερουν λειτουργικη ομαδα, συνθεσεις που τις πepιεχουν και οι χρησεις τους
BR9910130A (pt) Derivados de cubana como antagonistas de receptor de glutamato metabotrópico e processo para a sua preparação
AU2001213184A1 (en) A process of producing polyphenolic adhesive proteins and proteins produced in accordance with the process
WO2001012819A3 (en) Protein phosphatases and diagnosis and treatment of phosphatase-related disorders
BR0007638A (pt) Processo para determinação pós-genÈmica de estruturas atÈmicas macromoleculares
BR9809637A (pt) Glicoconjugados de 20(s)-camptotecina
BR9816100A (pt) Processo para produção de fosfatìdios desoleados
ATE405656T1 (de) Herstellungsverfahren für rekombinante proteine, plasmide und modifizierte zellen
HUP0004577A1 (hu) Kationos nukleinsav transzfekciós ágensek új csoportja
FI964186A0 (fi) Ei-liittävät gp350/220-variantit
HUP0100802A2 (hu) (1S,4R)-cisz-4-[2-amino-6-(ciklopropil-amino)-9H-purin-9-il]-2-ciklopentén-1-metanolt tartalmazó új gyógyszerkészítmények
BR0113623A (pt) Proteìnas sintéticas de estìmulo da eritropoiese

Legal Events

Date Code Title Description
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 10 (DEZ) ANOS CONTADOS A PARTIR DE 04/11/2008, OBSERVADAS AS CONDICOES LEGAIS.

B21A Patent or certificate of addition expired [chapter 21.1 patent gazette]

Free format text: PATENTE EXTINTA EM 04.11.2018