BR112022014376A2 - NEW MAIN FACILITATORS (MFS) SUPERFAMILY PROTEIN (FRED) IN HMO PRODUCTION - Google Patents

NEW MAIN FACILITATORS (MFS) SUPERFAMILY PROTEIN (FRED) IN HMO PRODUCTION

Info

Publication number
BR112022014376A2
BR112022014376A2 BR112022014376A BR112022014376A BR112022014376A2 BR 112022014376 A2 BR112022014376 A2 BR 112022014376A2 BR 112022014376 A BR112022014376 A BR 112022014376A BR 112022014376 A BR112022014376 A BR 112022014376A BR 112022014376 A2 BR112022014376 A2 BR 112022014376A2
Authority
BR
Brazil
Prior art keywords
mfs
fred
facilitators
new main
hmo
Prior art date
Application number
BR112022014376A
Other languages
Portuguese (pt)
Inventor
Pedersen Margit
D'arrigo Isotta
Bych Kampmann Katrine
Papadakis Manos
Original Assignee
Glycom As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Glycom As filed Critical Glycom As
Priority claimed from PCT/EP2021/051479 external-priority patent/WO2021148620A1/en
Publication of BR112022014376A2 publication Critical patent/BR112022014376A2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H3/00—Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
    • C07H3/06—Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

NOVA PROTEÍNA DA SUPERFAMÍLIA DE FACILITADORES PRINCIPAIS (MFS) (FRED) NA PRODUÇÃO DE HMO. A presente invenção se refere ao campo da produção recombinante de moléculas biológicas em células geneticamente modificadas. Mais particularmente, refere-se a um método para a produção recombinante de oligossacarídeos do leite humano (HMO) utilizando uma célula geneticamente modificada que expressa uma proteína da superfamília de facilitadores principais (MFS), a proteína expressa sendo Fred.NEW MAIN FACILITATORS (MFS) SUPERFAMILY PROTEIN (FRED) IN HMO PRODUCTION. The present invention relates to the field of recombinant production of biological molecules in genetically modified cells. More particularly, it relates to a method for the recombinant production of human milk oligosaccharides (HMO) using a genetically modified cell that expresses a major enhancer superfamily (MFS) protein, the expressed protein being Fred.

BR112022014376A 2020-01-23 2021-01-22 NEW MAIN FACILITATORS (MFS) SUPERFAMILY PROTEIN (FRED) IN HMO PRODUCTION BR112022014376A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA202000087 2020-01-23
PCT/EP2021/051479 WO2021148620A1 (en) 2020-01-23 2021-01-22 New major facilitator superfamily (mfs) protein (fred) in hmo production

Publications (1)

Publication Number Publication Date
BR112022014376A2 true BR112022014376A2 (en) 2022-10-04

Family

ID=83023792

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112022014376A BR112022014376A2 (en) 2020-01-23 2021-01-22 NEW MAIN FACILITATORS (MFS) SUPERFAMILY PROTEIN (FRED) IN HMO PRODUCTION

Country Status (5)

Country Link
US (1) US20230109937A1 (en)
EP (1) EP4093876A1 (en)
JP (1) JP2023511525A (en)
CN (1) CN115003813A (en)
BR (1) BR112022014376A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN118056902A (en) * 2022-11-21 2024-05-21 山东恒鲁生物科技有限公司 Mature polypeptide sequence for synthesizing HMOs and application thereof

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2715010T3 (en) * 2014-03-31 2019-05-31 Jennewein Biotechnologie Gmbh Total oligosaccharide fermentation
CN107108677B (en) * 2014-10-24 2020-08-07 格礼卡姆股份公司 Mixtures of HMOs
EP3141610A1 (en) * 2015-09-12 2017-03-15 Jennewein Biotechnologie GmbH Production of human milk oligosaccharides in microbial hosts with engineered import / export
CN111727253A (en) * 2017-12-21 2020-09-29 格礼卡姆股份公司 Nucleic acid constructs for in vitro and in vivo gene expression
CN109609422A (en) * 2018-11-20 2019-04-12 安徽天凯生物科技有限公司 One kind can produce the construction method of 2 '-rock algae base lactose bacterial strains

Also Published As

Publication number Publication date
US20230109937A1 (en) 2023-04-13
JP2023511525A (en) 2023-03-20
CN115003813A (en) 2022-09-02
EP4093876A1 (en) 2022-11-30

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Legal Events

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B11A Dismissal acc. art.33 of ipl - examination not requested within 36 months of filing
B11Y Definitive dismissal - extension of time limit for request of examination expired [chapter 11.1.1 patent gazette]