BR112022012105A2 - Variantes de xilanase e polinucleotídeos codificando as mesmas - Google Patents

Variantes de xilanase e polinucleotídeos codificando as mesmas

Info

Publication number
BR112022012105A2
BR112022012105A2 BR112022012105A BR112022012105A BR112022012105A2 BR 112022012105 A2 BR112022012105 A2 BR 112022012105A2 BR 112022012105 A BR112022012105 A BR 112022012105A BR 112022012105 A BR112022012105 A BR 112022012105A BR 112022012105 A2 BR112022012105 A2 BR 112022012105A2
Authority
BR
Brazil
Prior art keywords
variants
polynucleotides encoding
xylanase
present
xylanase variants
Prior art date
Application number
BR112022012105A
Other languages
English (en)
Inventor
Saikia Rakhi
Vaijanathappa Shivange Amol
DALAL Sohel
Jensen Kenneth
Olsen Lars
Bertel Rømer M Krogh Kristian
Mallison Shoup Madelyn
Vidal Bernardo Jr
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Publication of BR112022012105A2 publication Critical patent/BR112022012105A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C12N9/2482—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
    • D—TEXTILES; PAPER
    • D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
    • D21C—PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR
    • D21C5/00—Other processes for obtaining cellulose, e.g. cooking cotton linters ; Processes characterised by the choice of cellulose-containing starting materials
    • D21C5/005—Treatment of cellulose-containing material with microorganisms or enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

VARIANTES DE XILANASE E POLINUCLEOTÍDEOS CODIFICANDO AS MESMAS. A presente invenção se relaciona com variantes de xilanase de uma xilanase genitora tendo termoestabilidade aumentada em comparação com a xilanase genitora. A presente invenção se relaciona também com polinucleotídeos codificando as variantes, construtos de ácido nucleico, vetores e células hospedeiras compreendendo os polinucleotídeos, e método de produção das variantes da presente invenção.
BR112022012105A 2019-12-19 2020-12-17 Variantes de xilanase e polinucleotídeos codificando as mesmas BR112022012105A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN201941052984 2019-12-19
PCT/EP2020/086601 WO2021122867A2 (en) 2019-12-19 2020-12-17 Xylanase variants and polynucleotides encoding same

Publications (1)

Publication Number Publication Date
BR112022012105A2 true BR112022012105A2 (pt) 2022-12-13

Family

ID=74181105

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112022012105A BR112022012105A2 (pt) 2019-12-19 2020-12-17 Variantes de xilanase e polinucleotídeos codificando as mesmas

Country Status (9)

Country Link
US (1) US20230017381A1 (pt)
EP (1) EP4077654A2 (pt)
JP (1) JP2023506305A (pt)
KR (1) KR20220118469A (pt)
CN (1) CN114761551A (pt)
AR (1) AR120801A1 (pt)
BR (1) BR112022012105A2 (pt)
MX (1) MX2022007450A (pt)
WO (1) WO2021122867A2 (pt)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113652409B (zh) * 2021-08-11 2024-05-07 河北维达康生物科技有限公司 一种新的甘草次酸葡糖醛酸基转移酶突变体及其应用
CN113583996B (zh) * 2021-09-29 2022-01-14 翌圣生物科技(上海)股份有限公司 Bst DNA聚合酶重组突变体、其编码DNA及超快磁珠LAMP检测方法
WO2024011085A1 (en) 2022-07-05 2024-01-11 Novozymes A/S Method for wet-milling and related control system

Family Cites Families (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
US5989870A (en) 1986-04-30 1999-11-23 Rohm Enzyme Finland Oy Method for cloning active promoters
US5066218A (en) 1987-05-13 1991-11-19 Genencor International, Inc. Composition of a steeped starched-containing grain and a cellulase enzyme
US5223409A (en) 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
EP0477280B1 (en) 1989-06-13 1996-09-11 Genencor International, Inc. A method for killing cells without cell lysis
IL99552A0 (en) 1990-09-28 1992-08-18 Ixsys Inc Compositions containing procaryotic cells,a kit for the preparation of vectors useful for the coexpression of two or more dna sequences and methods for the use thereof
FR2704860B1 (fr) 1993-05-05 1995-07-13 Pasteur Institut Sequences de nucleotides du locus cryiiia pour le controle de l'expression de sequences d'adn dans un hote cellulaire.
DE4343591A1 (de) 1993-12-21 1995-06-22 Evotec Biosystems Gmbh Verfahren zum evolutiven Design und Synthese funktionaler Polymere auf der Basis von Formenelementen und Formencodes
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
MX9606013A (es) 1994-06-03 1997-12-31 Novo Nordisk Biotech Inc Lacasas purificadas de myceliophthora y acidos nucleicos que codifican para las mismas.
EP0777737B1 (en) 1994-06-30 2005-05-04 Novozymes Biotech, Inc. Non-toxic, non-toxigenic, non-pathogenic fusarium expression system and promoters and terminators for use therein
US5955310A (en) 1998-02-26 1999-09-21 Novo Nordisk Biotech, Inc. Methods for producing a polypeptide in a bacillus cell
ATE332968T1 (de) 1998-10-26 2006-08-15 Novozymes As Erstellung und durchmusterung von interessierenden dna-banken in zellen von filamentösen pilzen
EP2278016B1 (en) 1999-03-22 2012-09-26 Novozymes Inc. Promoter sequences derived from Fusarium Venenatum and uses thereof
US6566125B2 (en) 2000-06-02 2003-05-20 The United States Of America As Represented By The Secretary Of Agriculture Use of enzymes to reduce steep time and SO2 requirements in a maize wet-milling process
WO2002000731A1 (en) 2000-06-28 2002-01-03 Novozymes A/S An improved process for providing a starch product, treating milled or grinded crop kernels with an aqueous solution with an acidiic protease activity
WO2002000911A1 (en) 2000-06-29 2002-01-03 Novozymes A/S Starch gluten separation process
AU2001265827A1 (en) 2000-06-30 2002-01-14 Novozymes A/S A process for washing a starch slurry, using an aqueous solution with an acidic protease activity
AU2002319696B2 (en) 2001-07-27 2007-11-01 The Government Of The United States Of America As Represented By The Secretary Of Health And Human Services Systems for in vivo site-directed mutagenesis using oligonucleotides
WO2010039889A2 (en) 2008-09-30 2010-04-08 Novozymes, Inc. Methods for using positively and negatively selectable genes in a filamentous fungal cell
DK2398889T3 (en) 2009-02-20 2018-07-30 Danisco Us Inc FERMENTATION BOUILLON FORMULATIONS
CN102775504B (zh) * 2011-05-11 2014-07-02 白银赛诺生物科技有限公司 一种酶法生产玉米淀粉的方法
US20160002690A1 (en) 2012-11-27 2016-01-07 Novozymes A/S Milling Process
MX2015006569A (es) 2012-11-27 2015-08-05 Novozymes As Proceso de molienda.
ES2757053T3 (es) * 2014-07-10 2020-04-28 Novozymes As Polipéptidos con actividad de xilanasa y polinucleótidos que codifican los mismos
ES2971299T3 (es) * 2017-07-24 2024-06-04 Novozymes As GH5 y GH30 en la molienda húmeda
BR112022002203A2 (pt) * 2019-08-05 2022-09-06 Novozymes As Misturas de enzimas e processos para produção de um ingrediente alimentar com alto teor de proteína a partir de um subproduto de vinhaça completa

Also Published As

Publication number Publication date
CN114761551A (zh) 2022-07-15
EP4077654A2 (en) 2022-10-26
AR120801A1 (es) 2022-03-16
KR20220118469A (ko) 2022-08-25
WO2021122867A3 (en) 2021-09-23
JP2023506305A (ja) 2023-02-15
MX2022007450A (es) 2022-06-27
US20230017381A1 (en) 2023-01-19
WO2021122867A2 (en) 2021-06-24

Similar Documents

Publication Publication Date Title
BR112022012105A2 (pt) Variantes de xilanase e polinucleotídeos codificando as mesmas
BR112017023975A2 (pt) variantes de alfa-amilase e polinucleotídeos codificando as mesmas
BR112022011955A2 (pt) Variantes de alfa-amilase
WO2018141707A3 (en) ALPHA-AMYLASE VARIANTS
MX2018013139A (es) Composiciones detergentes y sus usos.
MX2020004145A (es) Variantes de desoxirribonucleasa (dnasa).
EP3950941A3 (en) Dnase polypeptide variants
AU2017380488A8 (en) Polypeptides having lysozyme activity, polynucleotides encoding same and uses and compositions thereof
BR112019006388A2 (pt) enzimas modificadoras de ácido nucleico guiadas por rna e métodos de uso das mesmas
EP4219702A3 (en) Enzyme variants and polynucleotides encoding the same
MX2019008780A (es) Composiciones detergentes.
BR112012016320B8 (pt) Método para tratar material celulósico, polipeptídeo, molécula de ácido nucleico isolada, vetor de expressão recombinante, célula hospedeira, processos para produzir um polipeptídeo, e para obter uma preparação de enzima, preparação de enzima, e, uso do polipeptídeo ou da preparação de enzima
WO2012101206A3 (en) Novel glycoside hydrolases from thermophilic fungi
EP3091073A3 (en) Polypeptides having xylanase activity and polynucleotides encoding same
EP3805348A3 (en) Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
MX2019014889A (es) Polipeptidos que tienen actividad trehalasa y polinucleotidos que los codifican.
WO2015110473A3 (en) Pullulanase variants and polynucleotides encoding same
EA201990034A1 (ru) Ферментативное гликозилирование стевиолгликозидов и других соединений с глюкозо-1-фосфатом
AU2018264463A1 (en) Polypeptides having lysozyme activity, polynucleotides encoding same and uses and compositions thereof
WO2017103159A9 (en) Polypeptides having xylanase activity and polynucleotides encoding same
BR112017004233A2 (pt) polipeptídeos tendo atividade de xilanase com uma alta taxa de conversão de polissacarídeos contendo xilose
MX2015013447A (es) Polipeptidos que tienen actividad de la fosfolipasa a y polinucleotidos que los codifican.
BR112022014946A2 (pt) Variantes de mananase e polinucleotídeos codificando as mesmas
BR112017004495A2 (pt) variantes de celobiohidrolase e polinucleotídeos codificando as mesmas
EP4067485A3 (en) Lipase variants and polynucleotides encoding same