BR112021020144A2 - Transposição de construções de ácidos nucleicos em genomas eucarióticos com uma transposase de amyelois - Google Patents

Transposição de construções de ácidos nucleicos em genomas eucarióticos com uma transposase de amyelois

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Publication number
BR112021020144A2
BR112021020144A2 BR112021020144A BR112021020144A BR112021020144A2 BR 112021020144 A2 BR112021020144 A2 BR 112021020144A2 BR 112021020144 A BR112021020144 A BR 112021020144A BR 112021020144 A BR112021020144 A BR 112021020144A BR 112021020144 A2 BR112021020144 A2 BR 112021020144A2
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BR
Brazil
Prior art keywords
amyelois
transposase
transposition
nucleic acid
gene
Prior art date
Application number
BR112021020144A
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English (en)
Inventor
Jeremy Minshull
Maggie Lee
Sridhar Govindarajan
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Dna Twopointo Inc
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Publication date
Application filed by Dna Twopointo Inc filed Critical Dna Twopointo Inc
Publication of BR112021020144A2 publication Critical patent/BR112021020144A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/90—Vectors containing a transposable element
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/007—Vector systems having a special element relevant for transcription cell cycle specific enhancer/promoter combination

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Epidemiology (AREA)
  • Immunology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Ticket-Dispensing Machines (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

transposição de construções de ácidos nucleicos em genomas eucarióticos com uma transposase de amyelois. a presente invenção fornece vetores polinucleotídicos para alta expressão de genes heterólogos. alguns vetores compreendem adicionalmente novos transposons e transposases que melhoram ainda mais a expressão. além disso, são divulgados vetores que podem ser usados em um sistema de transferência de genes para a introdução estável de ácidos nucleicos no dna de uma célula. os sistemas de transferência de genes podem ser usados em métodos, por exemplo, expressão gênica, bioprocessamento, terapia gênica, mutagênese por inserção ou descoberta de genes.
BR112021020144A 2019-04-08 2020-04-07 Transposição de construções de ácidos nucleicos em genomas eucarióticos com uma transposase de amyelois BR112021020144A2 (pt)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201962831097P 2019-04-08 2019-04-08
US201962873342P 2019-07-12 2019-07-12
US202062990568P 2020-03-17 2020-03-17
PCT/US2020/027078 WO2020210237A1 (en) 2019-04-08 2020-04-07 Transposition of nucleic acid constructs into eukaryotic genomes with a transposase from amyelois

Publications (1)

Publication Number Publication Date
BR112021020144A2 true BR112021020144A2 (pt) 2021-12-14

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
BR112021020144A BR112021020144A2 (pt) 2019-04-08 2020-04-07 Transposição de construções de ácidos nucleicos em genomas eucarióticos com uma transposase de amyelois

Country Status (11)

Country Link
US (3) US11060109B2 (pt)
EP (1) EP3953479A4 (pt)
JP (1) JP7418469B2 (pt)
KR (1) KR20210144861A (pt)
CN (1) CN112513277B (pt)
AU (1) AU2020272667B2 (pt)
BR (1) BR112021020144A2 (pt)
CA (1) CA3125043A1 (pt)
IL (2) IL287088B2 (pt)
SG (1) SG11202107383RA (pt)
WO (1) WO2020210237A1 (pt)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11060086B2 (en) 2019-04-08 2021-07-13 Dna Twopointo Inc. Transposition of nucleic acids into eukaryotic genomes with a transposase from heliothis
CA3125047A1 (en) * 2019-04-08 2020-10-15 Dna Twopointo Inc. Integration of nucleic acid constructs into eukaryotic cells with a transposase from oryzias
CN112513277B (zh) 2019-04-08 2024-07-23 Dna2.0股份有限公司 使用来自螟蛾属的转座酶将核酸构建体转座入真核基因组
WO2021226141A1 (en) 2020-05-04 2021-11-11 Saliogen Therapeutics, Inc. Transposition-based therapies
WO2022204067A1 (en) * 2021-03-24 2022-09-29 Dna Twopointo Inc. Tetracycline-inducible expression systems
CN116445542A (zh) * 2022-01-10 2023-07-18 佛山汉腾生物科技有限公司 表达载体及其应用
WO2024068995A1 (en) 2022-09-30 2024-04-04 Boehringer Ingelheim International Gmbh Novel transposase system
CN116590341A (zh) * 2023-07-13 2023-08-15 山东维真生物科技有限公司 一种腺病毒-PiggyBac***及其制备方法和应用

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US7560542B2 (en) 1999-05-07 2009-07-14 Monsanto Technology Llc Nucleic acid molecule SEQ ID NO. 68811 and other molecules associated with plants
US6518064B1 (en) * 2000-03-29 2003-02-11 The Regents Of The University Of California Pink bollworm expression system for commercially valuable protein production
US7932088B1 (en) 2005-04-25 2011-04-26 University Of Notre Dame Du Lac High efficiency transformation of Plasmodium falciparum by the lepidopteran transposon, piggyBac
CN101297031B (zh) * 2005-05-14 2013-06-05 复旦大学 在脊椎动物中作为遗传操作和分析工具的piggyBac
US8741340B2 (en) 2009-01-23 2014-06-03 Nsgene A/S Cell lines and their use in encapsulated cell biodelivery
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ES2679995T3 (es) * 2011-03-11 2018-09-03 Vib Vzw Moléculas y métodos para la inhibición y detección de proteínas
WO2014191146A1 (en) * 2013-04-29 2014-12-04 Agrosavfe N.V. Agrochemical compositions comprising antibodies binding to sphingolipids
US10233454B2 (en) * 2014-04-09 2019-03-19 Dna2.0, Inc. DNA vectors, transposons and transposases for eukaryotic genome modification
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ES2891087T3 (es) * 2015-10-08 2022-01-26 Dna Twopointo Inc Vectores de ADN, trasposones y transposasas para la modificación del genoma eucariota
CA3125047A1 (en) * 2019-04-08 2020-10-15 Dna Twopointo Inc. Integration of nucleic acid constructs into eukaryotic cells with a transposase from oryzias
CN112513277B (zh) * 2019-04-08 2024-07-23 Dna2.0股份有限公司 使用来自螟蛾属的转座酶将核酸构建体转座入真核基因组
US11060086B2 (en) 2019-04-08 2021-07-13 Dna Twopointo Inc. Transposition of nucleic acids into eukaryotic genomes with a transposase from heliothis
US20230313187A1 (en) * 2020-09-04 2023-10-05 Dna Twopointo Inc. Modifications of mammalian cells using artificial micro-rna to alter their properties and the compositions of their products

Also Published As

Publication number Publication date
AU2020272667A1 (en) 2021-07-15
CN112513277A (zh) 2021-03-16
JP2022527016A (ja) 2022-05-27
US20210324408A1 (en) 2021-10-21
US11396662B2 (en) 2022-07-26
IL287088B2 (en) 2023-09-01
IL287088B1 (en) 2023-05-01
US11060109B2 (en) 2021-07-13
IL301621A (en) 2023-05-01
JP7418469B2 (ja) 2024-01-19
US20200318135A1 (en) 2020-10-08
AU2020272667B2 (en) 2023-07-06
EP3953479A4 (en) 2023-01-11
KR20210144861A (ko) 2021-11-30
EP3953479A1 (en) 2022-02-16
SG11202107383RA (en) 2021-08-30
WO2020210237A1 (en) 2020-10-15
CA3125043A1 (en) 2020-10-15
CN112513277B (zh) 2024-07-23
IL287088A (en) 2021-12-01
US20220411816A1 (en) 2022-12-29

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