BR112021020144A2 - Transposição de construções de ácidos nucleicos em genomas eucarióticos com uma transposase de amyelois - Google Patents
Transposição de construções de ácidos nucleicos em genomas eucarióticos com uma transposase de amyeloisInfo
- Publication number
- BR112021020144A2 BR112021020144A2 BR112021020144A BR112021020144A BR112021020144A2 BR 112021020144 A2 BR112021020144 A2 BR 112021020144A2 BR 112021020144 A BR112021020144 A BR 112021020144A BR 112021020144 A BR112021020144 A BR 112021020144A BR 112021020144 A2 BR112021020144 A2 BR 112021020144A2
- Authority
- BR
- Brazil
- Prior art keywords
- amyelois
- transposase
- transposition
- nucleic acid
- gene
- Prior art date
Links
- 102000008579 Transposases Human genes 0.000 title abstract 3
- 108010020764 Transposases Proteins 0.000 title abstract 3
- 108020004707 nucleic acids Proteins 0.000 title abstract 3
- 102000039446 nucleic acids Human genes 0.000 title abstract 3
- 150000007523 nucleic acids Chemical group 0.000 title abstract 3
- 241001259788 Amyelois Species 0.000 title abstract 2
- 230000017105 transposition Effects 0.000 title abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 4
- 230000014509 gene expression Effects 0.000 abstract 3
- 239000013598 vector Substances 0.000 abstract 3
- 238000010364 biochemical engineering Methods 0.000 abstract 1
- 238000001415 gene therapy Methods 0.000 abstract 1
- 238000002743 insertional mutagenesis Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 102000040430 polynucleotide Human genes 0.000 abstract 1
- 108091033319 polynucleotide Proteins 0.000 abstract 1
- 239000002157 polynucleotide Substances 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/07—Nucleotidyltransferases (2.7.7)
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/90—Vectors containing a transposable element
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/007—Vector systems having a special element relevant for transcription cell cycle specific enhancer/promoter combination
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Epidemiology (AREA)
- Immunology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Ticket-Dispensing Machines (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
transposição de construções de ácidos nucleicos em genomas eucarióticos com uma transposase de amyelois. a presente invenção fornece vetores polinucleotídicos para alta expressão de genes heterólogos. alguns vetores compreendem adicionalmente novos transposons e transposases que melhoram ainda mais a expressão. além disso, são divulgados vetores que podem ser usados em um sistema de transferência de genes para a introdução estável de ácidos nucleicos no dna de uma célula. os sistemas de transferência de genes podem ser usados em métodos, por exemplo, expressão gênica, bioprocessamento, terapia gênica, mutagênese por inserção ou descoberta de genes.
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201962831097P | 2019-04-08 | 2019-04-08 | |
US201962873342P | 2019-07-12 | 2019-07-12 | |
US202062990568P | 2020-03-17 | 2020-03-17 | |
PCT/US2020/027078 WO2020210237A1 (en) | 2019-04-08 | 2020-04-07 | Transposition of nucleic acid constructs into eukaryotic genomes with a transposase from amyelois |
Publications (1)
Publication Number | Publication Date |
---|---|
BR112021020144A2 true BR112021020144A2 (pt) | 2021-12-14 |
Family
ID=72663758
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112021020144A BR112021020144A2 (pt) | 2019-04-08 | 2020-04-07 | Transposição de construções de ácidos nucleicos em genomas eucarióticos com uma transposase de amyelois |
Country Status (11)
Country | Link |
---|---|
US (3) | US11060109B2 (pt) |
EP (1) | EP3953479A4 (pt) |
JP (1) | JP7418469B2 (pt) |
KR (1) | KR20210144861A (pt) |
CN (1) | CN112513277B (pt) |
AU (1) | AU2020272667B2 (pt) |
BR (1) | BR112021020144A2 (pt) |
CA (1) | CA3125043A1 (pt) |
IL (2) | IL287088B2 (pt) |
SG (1) | SG11202107383RA (pt) |
WO (1) | WO2020210237A1 (pt) |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US11060086B2 (en) | 2019-04-08 | 2021-07-13 | Dna Twopointo Inc. | Transposition of nucleic acids into eukaryotic genomes with a transposase from heliothis |
CA3125047A1 (en) * | 2019-04-08 | 2020-10-15 | Dna Twopointo Inc. | Integration of nucleic acid constructs into eukaryotic cells with a transposase from oryzias |
CN112513277B (zh) | 2019-04-08 | 2024-07-23 | Dna2.0股份有限公司 | 使用来自螟蛾属的转座酶将核酸构建体转座入真核基因组 |
WO2021226141A1 (en) | 2020-05-04 | 2021-11-11 | Saliogen Therapeutics, Inc. | Transposition-based therapies |
WO2022204067A1 (en) * | 2021-03-24 | 2022-09-29 | Dna Twopointo Inc. | Tetracycline-inducible expression systems |
CN116445542A (zh) * | 2022-01-10 | 2023-07-18 | 佛山汉腾生物科技有限公司 | 表达载体及其应用 |
WO2024068995A1 (en) | 2022-09-30 | 2024-04-04 | Boehringer Ingelheim International Gmbh | Novel transposase system |
CN116590341A (zh) * | 2023-07-13 | 2023-08-15 | 山东维真生物科技有限公司 | 一种腺病毒-PiggyBac***及其制备方法和应用 |
Family Cites Families (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7560542B2 (en) | 1999-05-07 | 2009-07-14 | Monsanto Technology Llc | Nucleic acid molecule SEQ ID NO. 68811 and other molecules associated with plants |
US6518064B1 (en) * | 2000-03-29 | 2003-02-11 | The Regents Of The University Of California | Pink bollworm expression system for commercially valuable protein production |
US7932088B1 (en) | 2005-04-25 | 2011-04-26 | University Of Notre Dame Du Lac | High efficiency transformation of Plasmodium falciparum by the lepidopteran transposon, piggyBac |
CN101297031B (zh) * | 2005-05-14 | 2013-06-05 | 复旦大学 | 在脊椎动物中作为遗传操作和分析工具的piggyBac |
US8741340B2 (en) | 2009-01-23 | 2014-06-03 | Nsgene A/S | Cell lines and their use in encapsulated cell biodelivery |
US20110311506A1 (en) | 2009-02-25 | 2011-12-22 | The Johns Hopkins University | Piggybac transposon variants and methods of use |
CN102803484A (zh) * | 2009-06-11 | 2012-11-28 | 大学共同利用机关法人情报·***研究机构 | 生产蛋白质的方法 |
EP2678434B1 (en) * | 2011-02-25 | 2018-11-07 | Recombinetics, Inc. | Genetically modified animals and methods for making the same |
ES2679995T3 (es) * | 2011-03-11 | 2018-09-03 | Vib Vzw | Moléculas y métodos para la inhibición y detección de proteínas |
WO2014191146A1 (en) * | 2013-04-29 | 2014-12-04 | Agrosavfe N.V. | Agrochemical compositions comprising antibodies binding to sphingolipids |
US10233454B2 (en) * | 2014-04-09 | 2019-03-19 | Dna2.0, Inc. | DNA vectors, transposons and transposases for eukaryotic genome modification |
US20150291975A1 (en) * | 2014-04-09 | 2015-10-15 | Dna2.0, Inc. | Enhanced nucleic acid constructs for eukaryotic gene expression |
ES2891087T3 (es) * | 2015-10-08 | 2022-01-26 | Dna Twopointo Inc | Vectores de ADN, trasposones y transposasas para la modificación del genoma eucariota |
CA3125047A1 (en) * | 2019-04-08 | 2020-10-15 | Dna Twopointo Inc. | Integration of nucleic acid constructs into eukaryotic cells with a transposase from oryzias |
CN112513277B (zh) * | 2019-04-08 | 2024-07-23 | Dna2.0股份有限公司 | 使用来自螟蛾属的转座酶将核酸构建体转座入真核基因组 |
US11060086B2 (en) | 2019-04-08 | 2021-07-13 | Dna Twopointo Inc. | Transposition of nucleic acids into eukaryotic genomes with a transposase from heliothis |
US20230313187A1 (en) * | 2020-09-04 | 2023-10-05 | Dna Twopointo Inc. | Modifications of mammalian cells using artificial micro-rna to alter their properties and the compositions of their products |
-
2020
- 2020-04-07 CN CN202080003853.4A patent/CN112513277B/zh active Active
- 2020-04-07 US US16/842,709 patent/US11060109B2/en active Active
- 2020-04-07 BR BR112021020144A patent/BR112021020144A2/pt unknown
- 2020-04-07 SG SG11202107383RA patent/SG11202107383RA/en unknown
- 2020-04-07 IL IL287088A patent/IL287088B2/en unknown
- 2020-04-07 CA CA3125043A patent/CA3125043A1/en active Pending
- 2020-04-07 EP EP20787187.2A patent/EP3953479A4/en active Pending
- 2020-04-07 KR KR1020217035246A patent/KR20210144861A/ko not_active Application Discontinuation
- 2020-04-07 AU AU2020272667A patent/AU2020272667B2/en active Active
- 2020-04-07 WO PCT/US2020/027078 patent/WO2020210237A1/en unknown
- 2020-04-07 IL IL301621A patent/IL301621A/en unknown
- 2020-04-07 JP JP2021560152A patent/JP7418469B2/ja active Active
-
2021
- 2021-06-04 US US17/339,597 patent/US11396662B2/en active Active
-
2022
- 2022-06-22 US US17/847,021 patent/US20220411816A1/en active Pending
Also Published As
Publication number | Publication date |
---|---|
AU2020272667A1 (en) | 2021-07-15 |
CN112513277A (zh) | 2021-03-16 |
JP2022527016A (ja) | 2022-05-27 |
US20210324408A1 (en) | 2021-10-21 |
US11396662B2 (en) | 2022-07-26 |
IL287088B2 (en) | 2023-09-01 |
IL287088B1 (en) | 2023-05-01 |
US11060109B2 (en) | 2021-07-13 |
IL301621A (en) | 2023-05-01 |
JP7418469B2 (ja) | 2024-01-19 |
US20200318135A1 (en) | 2020-10-08 |
AU2020272667B2 (en) | 2023-07-06 |
EP3953479A4 (en) | 2023-01-11 |
KR20210144861A (ko) | 2021-11-30 |
EP3953479A1 (en) | 2022-02-16 |
SG11202107383RA (en) | 2021-08-30 |
WO2020210237A1 (en) | 2020-10-15 |
CA3125043A1 (en) | 2020-10-15 |
CN112513277B (zh) | 2024-07-23 |
IL287088A (en) | 2021-12-01 |
US20220411816A1 (en) | 2022-12-29 |
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