BR112021018124A2 - Cell line comprising selection marker and uses thereof for protein production - Google Patents

Cell line comprising selection marker and uses thereof for protein production

Info

Publication number
BR112021018124A2
BR112021018124A2 BR112021018124A BR112021018124A BR112021018124A2 BR 112021018124 A2 BR112021018124 A2 BR 112021018124A2 BR 112021018124 A BR112021018124 A BR 112021018124A BR 112021018124 A BR112021018124 A BR 112021018124A BR 112021018124 A2 BR112021018124 A2 BR 112021018124A2
Authority
BR
Brazil
Prior art keywords
cell line
selection marker
protein production
production
dhodh
Prior art date
Application number
BR112021018124A
Other languages
Portuguese (pt)
Inventor
Louis DUMAS Bruno
Nabil LOUNIS Mohammed
Original Assignee
Sanofi Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sanofi Sa filed Critical Sanofi Sa
Publication of BR112021018124A2 publication Critical patent/BR112021018124A2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
    • C12Y103/05—Oxidoreductases acting on the CH-CH group of donors (1.3) with a quinone or related compound as acceptor (1.3.5)
    • C12Y103/05002—Dihydroorotate dehydrogenase (1.3.5.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

linhagem celular compreendendo marcador de seleção e usos do mesmo para produção de proteína. a presente invenção refere-se a uma linhagem celular que compreende um gene de desidro-orotato desidrogenase endógena (dhodh) que está parcial ou totalmente inativado e seu uso para produção de proteínas recombinantes.cell line comprising selection marker and uses thereof for protein production. The present invention relates to a cell line comprising an endogenous dehydro-orotate dehydrogenase (dhodh) gene that is partially or totally inactivated and its use for the production of recombinant proteins.

BR112021018124A 2019-03-19 2020-03-19 Cell line comprising selection marker and uses thereof for protein production BR112021018124A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP19305331 2019-03-19
PCT/EP2020/057583 WO2020188021A1 (en) 2019-03-19 2020-03-19 Novel selection marker-comprising cell line and uses thereof for protein production

Publications (1)

Publication Number Publication Date
BR112021018124A2 true BR112021018124A2 (en) 2021-11-16

Family

ID=66001151

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112021018124A BR112021018124A2 (en) 2019-03-19 2020-03-19 Cell line comprising selection marker and uses thereof for protein production

Country Status (14)

Country Link
US (1) US20220177909A1 (en)
EP (1) EP3942054A1 (en)
JP (1) JP2022526497A (en)
KR (1) KR20210141511A (en)
CN (1) CN113811615A (en)
AU (1) AU2020242966A1 (en)
BR (1) BR112021018124A2 (en)
CA (1) CA3134116A1 (en)
IL (1) IL286419A (en)
MX (1) MX2021011303A (en)
SG (1) SG11202110105UA (en)
TW (1) TW202102669A (en)
WO (1) WO2020188021A1 (en)
ZA (1) ZA202106718B (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TW202227622A (en) * 2020-09-21 2022-07-16 法商賽諾菲公司 Generation of a high producing recombinant chinese hamster ovary cell line for therapeutic protein production

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8717430D0 (en) 1987-07-23 1987-08-26 Celltech Ltd Recombinant dna product
KR100267720B1 (en) 1997-12-29 2001-03-02 박호군 An expression vector containing human glutamine synthase gene and a producing method of erythropoietin from animal cell using the same
SG11201702516UA (en) 2014-10-23 2017-04-27 Sanofi Sa Novel selection marker for cell transfection and protein production

Also Published As

Publication number Publication date
MX2021011303A (en) 2021-10-22
KR20210141511A (en) 2021-11-23
US20220177909A1 (en) 2022-06-09
CA3134116A1 (en) 2020-09-24
ZA202106718B (en) 2023-04-26
EP3942054A1 (en) 2022-01-26
IL286419A (en) 2021-10-31
TW202102669A (en) 2021-01-16
WO2020188021A1 (en) 2020-09-24
JP2022526497A (en) 2022-05-25
CN113811615A (en) 2021-12-17
SG11202110105UA (en) 2021-10-28
AU2020242966A1 (en) 2021-10-21

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