BR112018008546A2 - métodos para detecção de tamanho de ácido nucleico de sequências de repetição - Google Patents

métodos para detecção de tamanho de ácido nucleico de sequências de repetição

Info

Publication number
BR112018008546A2
BR112018008546A2 BR112018008546A BR112018008546A BR112018008546A2 BR 112018008546 A2 BR112018008546 A2 BR 112018008546A2 BR 112018008546 A BR112018008546 A BR 112018008546A BR 112018008546 A BR112018008546 A BR 112018008546A BR 112018008546 A2 BR112018008546 A2 BR 112018008546A2
Authority
BR
Brazil
Prior art keywords
nucleic acid
methods
repeat sequences
detecting nucleic
acid size
Prior art date
Application number
BR112018008546A
Other languages
English (en)
Inventor
Hadd Andrew
Printy Blake
Bram Eran
Shroff Raghav
Original Assignee
Asuragen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Asuragen Inc filed Critical Asuragen Inc
Publication of BR112018008546A2 publication Critical patent/BR112018008546A2/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/151—Modifications characterised by repeat or repeated sequences, e.g. VNTR, microsatellite, concatemer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2545/00—Reactions characterised by their quantitative nature
    • C12Q2545/10—Reactions characterised by their quantitative nature the purpose being quantitative analysis
    • C12Q2545/101—Reactions characterised by their quantitative nature the purpose being quantitative analysis with an internal standard/control
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2545/00—Reactions characterised by their quantitative nature
    • C12Q2545/10—Reactions characterised by their quantitative nature the purpose being quantitative analysis
    • C12Q2545/113—Reactions characterised by their quantitative nature the purpose being quantitative analysis with an external standard/control, i.e. control reaction is separated from the test/target reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/10—Detection mode being characterised by the assay principle
    • C12Q2565/125—Electrophoretic separation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

são aqui descritos sistemas e dispositivos de anotação de cálculo e processamento de dados, e métodos correspondentes, para análise de ácido nucleico. em particular, são aqui descritos métodos para dimensionamento de uma região de repetição de uma amostra de ácido nucleico. por exemplo, os métodos descritos neste documento utilizam uma escala de produtos de amplificação para determinar o tamanho do ácido nucleico.
BR112018008546A 2015-11-03 2016-11-03 métodos para detecção de tamanho de ácido nucleico de sequências de repetição BR112018008546A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562250476P 2015-11-03 2015-11-03
PCT/US2016/060389 WO2017079478A1 (en) 2015-11-03 2016-11-03 Methods for nucleic acid size detection of repeat sequences

Publications (1)

Publication Number Publication Date
BR112018008546A2 true BR112018008546A2 (pt) 2018-10-30

Family

ID=58635460

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112018008546A BR112018008546A2 (pt) 2015-11-03 2016-11-03 métodos para detecção de tamanho de ácido nucleico de sequências de repetição

Country Status (10)

Country Link
US (2) US20170121763A1 (pt)
EP (1) EP3371330A4 (pt)
JP (1) JP7003035B2 (pt)
KR (1) KR20180064544A (pt)
CN (1) CN108431236B (pt)
AU (1) AU2016349391B2 (pt)
BR (1) BR112018008546A2 (pt)
CA (1) CA3002088A1 (pt)
IL (1) IL258727B2 (pt)
WO (1) WO2017079478A1 (pt)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9355167B2 (en) * 2012-05-18 2016-05-31 California Institute Of Technology Systems and methods for the distributed categorization of source data
US11449788B2 (en) 2017-03-17 2022-09-20 California Institute Of Technology Systems and methods for online annotation of source data using skill estimation
WO2021155114A1 (en) * 2020-01-30 2021-08-05 Laboratory Corporation Of America Holdings Fragile x syndrome agg interruption genotyping
CA3191872A1 (en) * 2020-08-15 2022-02-24 Life Technologies Corporation Dna analyzer with synthetic allelic ladder library
WO2024010875A1 (en) * 2022-07-06 2024-01-11 The Regents Of The University Of California Repeat-aware profiling of cell-free rna

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5853995A (en) * 1997-01-07 1998-12-29 Research Development Foundation Large scale genotyping of diseases and a diagnostic test for spinocerebellar ataxia type 6
US7593819B2 (en) 2001-07-11 2009-09-22 Applied Biosystems, Llc Internal calibration standards for electrophoretic analyses
US8409805B2 (en) 2009-02-13 2013-04-02 Asuragen, Inc. Method of amplification of GC-rich DNA templates
EP2411532B1 (en) 2009-03-24 2017-04-05 Asuragen, Inc. Pcr methods for characterizing the 5' untranslated region of the fmr1 and fmr2 genes
WO2012058633A1 (en) 2010-10-29 2012-05-03 Latham Gary J mPCR METHODS FOR ANALYZING REPEAT SEQUENCES
EP2888688B1 (en) 2012-07-20 2019-09-04 Asuragen, INC. Comprehensive fmr1 genotyping
US10497463B2 (en) 2013-11-13 2019-12-03 Myriad Women's Health, Inc. Automated nucleic acid repeat count calling methods

Also Published As

Publication number Publication date
CA3002088A1 (en) 2017-05-11
IL258727B2 (en) 2024-01-01
WO2017079478A1 (en) 2017-05-11
AU2016349391B2 (en) 2022-12-08
AU2016349391A1 (en) 2018-05-10
KR20180064544A (ko) 2018-06-14
IL258727B1 (en) 2023-09-01
US20200048696A1 (en) 2020-02-13
EP3371330A1 (en) 2018-09-12
CN108431236A (zh) 2018-08-21
US20170121763A1 (en) 2017-05-04
IL258727A (en) 2018-06-28
CN108431236B (zh) 2022-11-15
JP2019502367A (ja) 2019-01-31
JP7003035B2 (ja) 2022-02-10
US11913068B2 (en) 2024-02-27
EP3371330A4 (en) 2019-06-26

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