BR112018003576A2 - aumentando teor de óleo de planta através de alteração de um regulador negativo de acetil coa carboxilase - Google Patents
aumentando teor de óleo de planta através de alteração de um regulador negativo de acetil coa carboxilaseInfo
- Publication number
- BR112018003576A2 BR112018003576A2 BR112018003576A BR112018003576A BR112018003576A2 BR 112018003576 A2 BR112018003576 A2 BR 112018003576A2 BR 112018003576 A BR112018003576 A BR 112018003576A BR 112018003576 A BR112018003576 A BR 112018003576A BR 112018003576 A2 BR112018003576 A2 BR 112018003576A2
- Authority
- BR
- Brazil
- Prior art keywords
- regulator
- negative
- changing
- oil content
- coa carboxylase
- Prior art date
Links
- 102000000452 Acetyl-CoA carboxylase Human genes 0.000 title 1
- 108010016219 Acetyl-CoA carboxylase Proteins 0.000 title 1
- 108010018763 Biotin carboxylase Proteins 0.000 title 1
- 239000010773 plant oil Substances 0.000 title 1
- 238000000034 method Methods 0.000 abstract 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 abstract 2
- 235000014113 dietary fatty acids Nutrition 0.000 abstract 2
- 229930195729 fatty acid Natural products 0.000 abstract 2
- 239000000194 fatty acid Substances 0.000 abstract 2
- 150000004665 fatty acids Chemical class 0.000 abstract 2
- 241000195493 Cryptophyta Species 0.000 abstract 1
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 229960002685 biotin Drugs 0.000 abstract 1
- 235000020958 biotin Nutrition 0.000 abstract 1
- 239000011616 biotin Substances 0.000 abstract 1
- 230000003828 downregulation Effects 0.000 abstract 1
- 230000004576 lipid-binding Effects 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- DCXXMTOCNZCJGO-UHFFFAOYSA-N tristearoylglycerol Chemical compound CCCCCCCCCCCCCCCCCC(=O)OCC(OC(=O)CCCCCCCCCCCCCCCCC)COC(=O)CCCCCCCCCCCCCCCCC DCXXMTOCNZCJGO-UHFFFAOYSA-N 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
- C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/93—Ligases (6)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y604/00—Ligases forming carbon-carbon bonds (6.4)
- C12Y604/01—Ligases forming carbon-carbon bonds (6.4.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y604/00—Ligases forming carbon-carbon bonds (6.4)
- C12Y604/01—Ligases forming carbon-carbon bonds (6.4.1)
- C12Y604/01002—Acetyl-CoA carboxylase (6.4.1.2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/14—Type of nucleic acid interfering N.A.
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Nutrition Science (AREA)
- Botany (AREA)
- Virology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
a presente invenção refere-se ao provimento de um método e meios para alterar produção de ácido graxo e por último triacil glicerol emplantas e algas. métodos da invenção compreendem a etapa de alteração de níveis de atividade da etapa realizada para biossíntese de ácido graxo de novo. acetil-coa carboxilases (accase). mais especificamente, métodos da invenção aperfeiçoam diretamente a atividade de accase através de regulaçãodescendente de genes contendo domínio de ligação de biotina / lipoíla (badc) através de abrodagens de biotecnologia ou cultivo seletivo.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201562211371P | 2015-08-28 | 2015-08-28 | |
PCT/US2016/041386 WO2017039834A1 (en) | 2015-08-28 | 2016-07-07 | Increasing plant oil content by altering a negative regulator of acetyl-coa carboxylase |
Publications (1)
Publication Number | Publication Date |
---|---|
BR112018003576A2 true BR112018003576A2 (pt) | 2018-09-25 |
Family
ID=58188729
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112018003576A BR112018003576A2 (pt) | 2015-08-28 | 2016-07-07 | aumentando teor de óleo de planta através de alteração de um regulador negativo de acetil coa carboxilase |
Country Status (7)
Country | Link |
---|---|
US (2) | US10883113B2 (pt) |
EP (1) | EP3341486A4 (pt) |
CN (2) | CN108699568A (pt) |
AU (1) | AU2016317094B2 (pt) |
BR (1) | BR112018003576A2 (pt) |
CA (1) | CA2995573A1 (pt) |
WO (1) | WO2017039834A1 (pt) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2995573A1 (en) | 2015-08-28 | 2017-03-09 | The Curators Of The University Of Missouri | Increasing plant oil content by altering a negative regulator of acetyl-coa carboxylase |
CA3030170A1 (en) * | 2016-07-07 | 2018-01-11 | The Curators Of The University Of Missouri | Increasing plant oil content by improving activity of acetyl-coa carboxylase |
US20210230623A1 (en) * | 2018-05-30 | 2021-07-29 | The Curators Of The University Of Missouri | Methods of altering seed weight and seed oil content by manipulating alpha-carboxyl-transferase (a-ct) activity via carboxyl-transferase interaction (cti) protein expression |
CN112813092B (zh) * | 2020-08-06 | 2023-03-21 | 中国农业科学院棉花研究所 | GbBCCP5蛋白质及其编码基因在调控生物油脂含量中的应用 |
Family Cites Families (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB9306490D0 (en) | 1993-03-29 | 1993-05-19 | Zeneca Ltd | Plant gene specifying acetyl,coenzyme a carboxylase and transformed plants containing same |
AU4146297A (en) | 1996-08-02 | 1998-02-25 | Board Of Trustees Of Michigan State University | Structure and expression of the alpha-carboxyltransferase subunit of heteromeric-acetyl-coa carboxylase |
US20110281765A1 (en) * | 2000-03-29 | 2011-11-17 | Bush David F | Plant polymorphic markers and uses thereof |
JP3656104B2 (ja) | 2001-03-13 | 2005-06-08 | 国立大学法人 奈良先端科学技術大学院大学 | 植物において脂肪酸合成を促進させる方法 |
US20030110533A1 (en) * | 2001-07-06 | 2003-06-12 | Cahoon Rebecca E. | Acetyl-CoA carboxylase subunits |
AU2001289843A1 (en) * | 2001-08-28 | 2002-02-13 | Bayer Cropscience Ag | Polypeptides for identifying herbicidally active compounds |
DE102005013779A1 (de) * | 2005-03-22 | 2006-09-28 | Basf Plant Science Gmbh | Verfahren zur Herstellung von mehrfach ungesättigten C20- und C22-Fettsäuren mit mindestens vier Doppelbindungen in transgenen Pflanzen |
BR122020016899B1 (pt) * | 2007-04-09 | 2021-06-22 | Evogene Ltd | Método para aumentar o teor de óleo, taxa de crescimento, biomassa, vigor e/ou rendimento de uma planta, e, construção de ácido nucleico isolado |
PL222498B1 (pl) * | 2009-05-27 | 2016-08-31 | Inst Hodowli I Aklimatyzacji Roślin | Kaseta DNA, wektor binarny, szczep A. tumefaciens oraz sposób otrzymywania rośliny zbożowej o zwiększonej produktywności |
EP2725895A1 (en) | 2011-06-28 | 2014-05-07 | Brookhaven Science Associates LLC | Modified plants with increased oil content |
CA2995573A1 (en) | 2015-08-28 | 2017-03-09 | The Curators Of The University Of Missouri | Increasing plant oil content by altering a negative regulator of acetyl-coa carboxylase |
-
2016
- 2016-07-07 CA CA2995573A patent/CA2995573A1/en active Pending
- 2016-07-07 EP EP16842484.4A patent/EP3341486A4/en not_active Withdrawn
- 2016-07-07 CN CN201680057571.6A patent/CN108699568A/zh active Pending
- 2016-07-07 BR BR112018003576A patent/BR112018003576A2/pt not_active Application Discontinuation
- 2016-07-07 CN CN202310229890.2A patent/CN116121294A/zh active Pending
- 2016-07-07 AU AU2016317094A patent/AU2016317094B2/en active Active
- 2016-07-07 WO PCT/US2016/041386 patent/WO2017039834A1/en active Application Filing
- 2016-07-07 US US15/756,468 patent/US10883113B2/en active Active
-
2020
- 2020-11-04 US US17/089,241 patent/US11959087B2/en active Active
Also Published As
Publication number | Publication date |
---|---|
CN116121294A (zh) | 2023-05-16 |
US20180251775A1 (en) | 2018-09-06 |
AU2016317094B2 (en) | 2021-12-16 |
EP3341486A4 (en) | 2019-05-15 |
US11959087B2 (en) | 2024-04-16 |
AU2016317094A1 (en) | 2018-03-08 |
US20210171970A1 (en) | 2021-06-10 |
WO2017039834A1 (en) | 2017-03-09 |
EP3341486A1 (en) | 2018-07-04 |
CA2995573A1 (en) | 2017-03-09 |
CN108699568A (zh) | 2018-10-23 |
US10883113B2 (en) | 2021-01-05 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
BR112018003576A2 (pt) | aumentando teor de óleo de planta através de alteração de um regulador negativo de acetil coa carboxilase | |
MX2018004264A (es) | Metodo para producir plantas completas a partir de protoplastos. | |
EA202092987A2 (ru) | Липид, содержащий докозапентаеновую кислоту | |
AR106860A1 (es) | Proceso para incrementar la producción de biomasa y esporas de bacterias promotoras del crecimiento vegetal del género bacillus | |
BR112015024674A2 (pt) | método para aumentar a capacidade de sobrevivência de um ou mais microrganismos benéficos e para revestir uma semente, e, composição | |
UA111990C2 (uk) | Спосіб регуляції росту рослин | |
BR112013016837A2 (pt) | método para melhorar a qualidade de uma planta | |
MX2018014764A (es) | Metodo para producir acido 3-hidroxiadipico. | |
BR112016016539A2 (pt) | micro-organismos recombinantes enriquecidos com carboidratos | |
BR112018000794A2 (pt) | sistema de múltiplas espigas para aprimorar rendimento de planta monocotiledônea | |
BR112017012541A2 (pt) | supressão de rnai parental de gene hunchback para controlar pragas hemípteras | |
BR102014009838B8 (pt) | Método para produzir um l-aminoácido | |
MX2018014769A (es) | Metodo para producir acido a-hidromuconico. | |
UA117935C2 (uk) | Спосіб одержання l-амінокислот | |
EA201691383A1 (ru) | Рекомбинантные микроорганизмы и способы их применения | |
EA201490275A1 (ru) | Композиции и способы получения изопрена | |
BR112015001616A2 (pt) | intraesterificação 1,3-específica | |
BR112017011246A2 (pt) | composições e métodos para introdução de micronutriente | |
BR112016016397A2 (pt) | método para preparar 1-alquil-3-difluorometil-5-fluoro-1h-pirazol-4-carbaldeídos e 1-alquil-3-difluorometil-5-fluoro-1h-pirazol-4-carboxilatos. | |
BR112016018195A2 (pt) | micro-organismo modificado, método de produção de um composto orgânico e uso de um micro-organismo modificado | |
CO2020002617A2 (es) | Método para la producción de plántulas de caña de azúcar | |
WO2018009626A3 (en) | Increasing plant oil content by improving activity of acetyl-coa carboxylase | |
BR112014018009A2 (pt) | sequência de ácido nucleico isolada, construção recombinante, célula vegetal, método para reduzir a expressão de pelo menos um gene biossintético de ácido graxo vegetal, método para reduzir a expressão de dois ou mais genes biossintéticos de ácido graxo vegetal e planta ou semente transgênica | |
BR112016018096A2 (pt) | Método para produzir biocombustíveis renováveis leves | |
MX2018014918A (es) | Esteres de tetrahidropiranilalquilo inferior y su produccion usando un compuesto de ceteno. |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
B06U | Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette] | ||
B07A | Application suspended after technical examination (opinion) [chapter 7.1 patent gazette] | ||
B06A | Patent application procedure suspended [chapter 6.1 patent gazette] | ||
B11B | Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements |