BR112018002525A2 - detecção de antígenos usando marcadores fotocliváveis - Google Patents
detecção de antígenos usando marcadores fotocliváveisInfo
- Publication number
- BR112018002525A2 BR112018002525A2 BR112018002525A BR112018002525A BR112018002525A2 BR 112018002525 A2 BR112018002525 A2 BR 112018002525A2 BR 112018002525 A BR112018002525 A BR 112018002525A BR 112018002525 A BR112018002525 A BR 112018002525A BR 112018002525 A2 BR112018002525 A2 BR 112018002525A2
- Authority
- BR
- Brazil
- Prior art keywords
- photocleavable
- antigen
- antigen detection
- markers
- multiplex
- Prior art date
Links
- 239000000427 antigen Substances 0.000 title abstract 7
- 102000036639 antigens Human genes 0.000 title abstract 7
- 108091007433 antigens Proteins 0.000 title abstract 7
- 238000001514 detection method Methods 0.000 title abstract 3
- 238000000034 method Methods 0.000 abstract 2
- 239000003446 ligand Substances 0.000 abstract 1
- 239000003550 marker Substances 0.000 abstract 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/533—Production of labelled immunochemicals with fluorescent label
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54306—Solid-phase reaction mechanisms
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
- G01N1/312—Apparatus therefor for samples mounted on planar substrates
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/02—Adapting objects or devices to another
- B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0627—Sensor or part of a sensor is integrated
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/08—Regulating or influencing the flow resistance
- B01L2400/082—Active control of flow resistance, e.g. flow controllers
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N2001/302—Stain compositions
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
- G01N2035/00099—Characterised by type of test elements
- G01N2035/00138—Slides
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2458/00—Labels used in chemical analysis of biological material
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2458/00—Labels used in chemical analysis of biological material
- G01N2458/10—Oligonucleotides as tagging agents for labelling antibodies
Abstract
são aqui fornecidos métodos para o uso de marcadores fotocliváveis para a detecção multiplex e serial de antígenos. os métodos compreendem a detecção da presença de marcadores fotocliváveis, que são conjugados através de ligantes fun-cionais a complexos de ligação a antígeno, que, por sua vez, ligam-se não covalen-temente aos antígenos. a presença de um marcador fotoclivável é indicativa da pre-sença de um antígeno ligado especificamente ou seletivamente a um complexo de ligação ao antígeno. são aqui fornecidos, também, aparelhos para o uso de marca-ções fotocliváveis para a detecção multiplex e serial de antígenos.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201562201978P | 2015-08-06 | 2015-08-06 | |
PCT/US2016/045981 WO2017024298A1 (en) | 2015-08-06 | 2016-08-08 | Antigen detection using photocleavable labels |
Publications (1)
Publication Number | Publication Date |
---|---|
BR112018002525A2 true BR112018002525A2 (pt) | 2018-09-25 |
Family
ID=56686978
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112018002525A BR112018002525A2 (pt) | 2015-08-06 | 2016-08-08 | detecção de antígenos usando marcadores fotocliváveis |
Country Status (9)
Country | Link |
---|---|
US (2) | US10942176B2 (pt) |
EP (1) | EP3332253A1 (pt) |
JP (1) | JP6893914B2 (pt) |
CN (1) | CN107949790A (pt) |
AU (2) | AU2016304599A1 (pt) |
BR (1) | BR112018002525A2 (pt) |
CA (2) | CA3138794C (pt) |
MX (1) | MX2018001519A (pt) |
WO (1) | WO2017024298A1 (pt) |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20200306376A1 (en) * | 2017-04-25 | 2020-10-01 | Dana-Farber Cancer Institute, Inc. | Compositions and methods for targeted tumor immunotherapy |
EP3431173A1 (en) * | 2017-07-19 | 2019-01-23 | Bayer Pharma Aktiengesellschaft | Continuous manufacture of guidance molecule drug conjugates |
CN111801350A (zh) * | 2018-01-30 | 2020-10-20 | 拉什大学医学中心 | 用于组织和细胞的多重分析的顺序染色 |
US20210228699A1 (en) * | 2018-06-04 | 2021-07-29 | Dana-Farber Cancer Institute, Inc. | Compositions and methods for targeted immunotherapy |
US11739321B2 (en) | 2019-01-07 | 2023-08-29 | Agilent Technologies, Inc. | Compositions and methods for genomic DNA and gene expression analysis in single cells |
JP2020143015A (ja) * | 2019-03-06 | 2020-09-10 | ソニー株式会社 | 粒子標識用試薬、粒子染色方法、及び顕微鏡システム |
EP3983803A2 (en) * | 2019-06-14 | 2022-04-20 | Akoya Biosciences, Inc. | Multiplexed tissue imaging |
CN111381034A (zh) * | 2020-03-03 | 2020-07-07 | 武汉生之源生物科技股份有限公司 | 一种gpc-3抗体包被磁珠及其制备方法、试剂盒 |
WO2022076413A1 (en) * | 2020-10-05 | 2022-04-14 | Clara Biotech, Inc. | Apparatus, system, and method for analyte release |
CN112595845B (zh) * | 2020-12-09 | 2022-10-21 | 深圳普门科技股份有限公司 | 透明质酸检测试剂盒及检测*** |
Family Cites Families (18)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US41115A (en) * | 1864-01-05 | Edward b | ||
US6358689B1 (en) | 1994-05-11 | 2002-03-19 | Boston University | Detection of markers in nascent proteins |
US6589736B1 (en) | 1994-11-22 | 2003-07-08 | The Trustees Of Boston University | Photocleavable agents and conjugates for the detection and isolation of biomolecules |
CN2444230Y (zh) | 2000-05-16 | 2001-08-22 | 陈智 | 免疫组织化学仪 |
US7154598B2 (en) | 2002-07-12 | 2006-12-26 | Decision Biomarkers, Inc. | Excitation and imaging of fluorescent arrays |
EP1880223A2 (en) | 2005-04-21 | 2008-01-23 | Celerus Diagnostics, Inc. | Method and apparatus for automated rapid immunohistochemistry |
EP1968641B1 (en) | 2005-12-12 | 2014-06-11 | Innova Biosciences Limited | Production of conjugates |
RS54163B1 (en) | 2006-05-30 | 2015-12-31 | Genentech Inc. | ANTI-CD22 ANTIBODIES, THEIR IMMUNCONJUGATES AND THEIR USE |
US20090203023A1 (en) | 2008-02-11 | 2009-08-13 | University Of Wyoming | Concentrating Microorganisms in Aqueous Solution Prior to Selective Staining and Detection |
US20090253163A1 (en) | 2008-04-02 | 2009-10-08 | General Electric Company | Iterative staining of biological samples |
EP2307565B1 (en) | 2008-06-11 | 2017-11-29 | Lasergen, Inc. | Reversible nucleosides and nucleotides terminators and their use in dna sequencing |
DE102008062372B3 (de) * | 2008-12-17 | 2010-06-17 | Medizinische Hochschule Hannover | Nachweiskonjugat und Verfahren zur Analyse |
KR101066247B1 (ko) * | 2009-08-04 | 2011-09-20 | 서울대학교산학협력단 | 멀티 루프 코일을 구비하는 판재용 비접촉식 트랜스듀서 |
CN102230895B (zh) | 2011-03-31 | 2014-10-29 | 中国科学院合肥物质科学研究院 | 一种利用荧光蛋白作为荧光探针测量电离辐射下细胞氧化胁迫损伤的方法 |
EP3670523B1 (en) * | 2011-09-13 | 2021-08-11 | Agilent Technologies, Inc. | 3'-oh unblocked, fast photocleavable terminating nucleotides and their use in methods for nucleic acid sequencing |
KR101969847B1 (ko) * | 2012-10-30 | 2019-04-17 | 삼성전자주식회사 | 동일 검체에서 여러 항원을 검출하기 위한 순차적 다중 면역염색법 |
WO2015128272A2 (en) * | 2014-02-26 | 2015-09-03 | Ventana Medical Systems, Inc. | Photo-selective method for biological sample analysis field |
WO2017015099A1 (en) | 2015-07-17 | 2017-01-26 | Nanostring Technologies, Inc. | Simultaneous quantification of gene expression in a user-defined region of a cross-sectioned tissue |
-
2016
- 2016-08-08 CN CN201680051653.XA patent/CN107949790A/zh active Pending
- 2016-08-08 JP JP2018506118A patent/JP6893914B2/ja active Active
- 2016-08-08 CA CA3138794A patent/CA3138794C/en active Active
- 2016-08-08 US US15/230,876 patent/US10942176B2/en active Active
- 2016-08-08 CA CA2993993A patent/CA2993993C/en active Active
- 2016-08-08 MX MX2018001519A patent/MX2018001519A/es unknown
- 2016-08-08 BR BR112018002525A patent/BR112018002525A2/pt unknown
- 2016-08-08 EP EP16751795.2A patent/EP3332253A1/en active Pending
- 2016-08-08 WO PCT/US2016/045981 patent/WO2017024298A1/en active Application Filing
- 2016-08-08 AU AU2016304599A patent/AU2016304599A1/en not_active Abandoned
-
2021
- 2021-02-12 US US17/174,860 patent/US20210181191A1/en not_active Abandoned
-
2022
- 2022-08-01 AU AU2022210222A patent/AU2022210222A1/en active Pending
Also Published As
Publication number | Publication date |
---|---|
US20170052175A1 (en) | 2017-02-23 |
US10942176B2 (en) | 2021-03-09 |
MX2018001519A (es) | 2018-08-01 |
CN107949790A (zh) | 2018-04-20 |
CA3138794A1 (en) | 2017-02-09 |
US20210181191A1 (en) | 2021-06-17 |
AU2022210222A1 (en) | 2022-08-25 |
WO2017024298A1 (en) | 2017-02-09 |
CA3138794C (en) | 2024-01-23 |
CA2993993A1 (en) | 2017-02-09 |
JP2018523826A (ja) | 2018-08-23 |
JP6893914B2 (ja) | 2021-06-23 |
AU2016304599A1 (en) | 2018-02-15 |
CA2993993C (en) | 2022-01-04 |
EP3332253A1 (en) | 2018-06-13 |
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Legal Events
Date | Code | Title | Description |
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B06U | Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette] | ||
B25A | Requested transfer of rights approved |
Owner name: AGILENT TECHNOLOGIES INC. (US) |