BR112017026719A8 - Nucleases cas9 termostáveis - Google Patents
Nucleases cas9 termostáveisInfo
- Publication number
- BR112017026719A8 BR112017026719A8 BR112017026719A BR112017026719A BR112017026719A8 BR 112017026719 A8 BR112017026719 A8 BR 112017026719A8 BR 112017026719 A BR112017026719 A BR 112017026719A BR 112017026719 A BR112017026719 A BR 112017026719A BR 112017026719 A8 BR112017026719 A8 BR 112017026719A8
- Authority
- BR
- Brazil
- Prior art keywords
- cas9 nucleases
- nucleic acid
- degrees celsius
- cas protein
- genetic engineering
- Prior art date
Links
- 108091033409 CRISPR Proteins 0.000 title abstract 4
- 238000010353 genetic engineering Methods 0.000 abstract 3
- 101710163270 Nuclease Proteins 0.000 abstract 2
- 108020004707 nucleic acids Proteins 0.000 abstract 2
- 102000039446 nucleic acids Human genes 0.000 abstract 2
- 150000007523 nucleic acids Chemical class 0.000 abstract 2
- 229920001184 polypeptide Polymers 0.000 abstract 2
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 2
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 102000004169 proteins and genes Human genes 0.000 abstract 2
- 230000007018 DNA scission Effects 0.000 abstract 1
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 1
- 239000013604 expression vector Substances 0.000 abstract 1
- 239000012634 fragment Substances 0.000 abstract 1
- 244000005700 microbiome Species 0.000 abstract 1
- 230000004048 modification Effects 0.000 abstract 1
- 238000012986 modification Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/21—Endodeoxyribonucleases producing 5'-phosphomonoesters (3.1.21)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
- Cell Biology (AREA)
- Mycology (AREA)
- Crystallography & Structural Chemistry (AREA)
Abstract
NUCLEASES CAS9 TERMOSTÁVEIS. A presente invenção refere-se ao campo da engenharia genética e, mais particularmente, à edição de ácido nucleico e modificação do genoma. A presente invenção proporciona uma proteína Cas isolada ou fragmento de polipeptídeo da mesma tendo uma sequência de aminoácidos de SEQ ID NO: 1 ou uma sequência de pelo menos 77% de identidade com a mesma, em que a proteína Cas ou o polipeptídeo é capaz de clivagem de DNA a uma temperatura na variam 50(graus celsius) e 100(graus celsius) inclusive. A invenção proporciona ainda moléculas de ácido nucleico isoladas que codificam as referidas nucleases Cas9, vetores de expressão e células hospedeiras. As nucleases Cas9 aqui descritas fornecem novas ferramentas para engenharia genética a temperaturas elevadas e têm um valor particular na manipulação genética de organismos termofílicos; particularmente microrganismos.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB1510296.5 | 2015-06-12 | ||
| GBGB1510296.5A GB201510296D0 (en) | 2015-06-12 | 2015-06-12 | Thermostable CAS9 nucleases |
| PCT/EP2016/062817 WO2016198361A1 (en) | 2015-06-12 | 2016-06-06 | Thermostable cas9 nucleases |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| BR112017026719A2 BR112017026719A2 (pt) | 2018-08-28 |
| BR112017026719A8 true BR112017026719A8 (pt) | 2022-07-05 |
Family
ID=53784593
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112017026719A BR112017026719A8 (pt) | 2015-06-12 | 2016-06-06 | Nucleases cas9 termostáveis |
Country Status (12)
| Country | Link |
|---|---|
| US (2) | US10731142B2 (pt) |
| EP (1) | EP3307883A1 (pt) |
| JP (1) | JP7088528B2 (pt) |
| KR (1) | KR20180019655A (pt) |
| CN (1) | CN107922931B (pt) |
| AU (1) | AU2016274452C1 (pt) |
| BR (1) | BR112017026719A8 (pt) |
| CA (1) | CA2989084A1 (pt) |
| EA (1) | EA038500B1 (pt) |
| GB (1) | GB201510296D0 (pt) |
| PH (1) | PH12017502281A1 (pt) |
| WO (1) | WO2016198361A1 (pt) |
Families Citing this family (36)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3613852A3 (en) | 2011-07-22 | 2020-04-22 | President and Fellows of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
| US20150044192A1 (en) | 2013-08-09 | 2015-02-12 | President And Fellows Of Harvard College | Methods for identifying a target site of a cas9 nuclease |
| US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
| US9322037B2 (en) | 2013-09-06 | 2016-04-26 | President And Fellows Of Harvard College | Cas9-FokI fusion proteins and uses thereof |
| US9737604B2 (en) | 2013-09-06 | 2017-08-22 | President And Fellows Of Harvard College | Use of cationic lipids to deliver CAS9 |
| US9228207B2 (en) | 2013-09-06 | 2016-01-05 | President And Fellows Of Harvard College | Switchable gRNAs comprising aptamers |
| US9068179B1 (en) | 2013-12-12 | 2015-06-30 | President And Fellows Of Harvard College | Methods for correcting presenilin point mutations |
| AU2015298571B2 (en) | 2014-07-30 | 2020-09-03 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
| GB201510296D0 (en) * | 2015-06-12 | 2015-07-29 | Univ Wageningen | Thermostable CAS9 nucleases |
| CN108513575A (zh) | 2015-10-23 | 2018-09-07 | 哈佛大学的校长及成员们 | 核碱基编辑器及其用途 |
| WO2018027078A1 (en) | 2016-08-03 | 2018-02-08 | President And Fellows Of Harard College | Adenosine nucleobase editors and uses thereof |
| CA3033327A1 (en) | 2016-08-09 | 2018-02-15 | President And Fellows Of Harvard College | Programmable cas9-recombinase fusion proteins and uses thereof |
| WO2018039438A1 (en) | 2016-08-24 | 2018-03-01 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| KR20240007715A (ko) | 2016-10-14 | 2024-01-16 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 핵염기 에디터의 aav 전달 |
| EP3555275A1 (en) * | 2016-12-14 | 2019-10-23 | Wageningen Universiteit | Thermostable cas9 nucleases |
| WO2018109101A1 (en) * | 2016-12-14 | 2018-06-21 | Wageningen Universiteit | Thermostable cas9 nucleases |
| US10745677B2 (en) | 2016-12-23 | 2020-08-18 | President And Fellows Of Harvard College | Editing of CCR5 receptor gene to protect against HIV infection |
| EP3592853A1 (en) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Suppression of pain by gene editing |
| JP2020510439A (ja) | 2017-03-10 | 2020-04-09 | プレジデント アンド フェローズ オブ ハーバード カレッジ | シトシンからグアニンへの塩基編集因子 |
| SG11201908658TA (en) | 2017-03-23 | 2019-10-30 | Harvard College | Nucleobase editors comprising nucleic acid programmable dna binding proteins |
| US11560566B2 (en) | 2017-05-12 | 2023-01-24 | President And Fellows Of Harvard College | Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation |
| US11692184B2 (en) | 2017-05-16 | 2023-07-04 | The Regents Of The University Of California | Thermostable RNA-guided endonucleases and methods of use thereof |
| US11732274B2 (en) | 2017-07-28 | 2023-08-22 | President And Fellows Of Harvard College | Methods and compositions for evolving base editors using phage-assisted continuous evolution (PACE) |
| US11319532B2 (en) | 2017-08-30 | 2022-05-03 | President And Fellows Of Harvard College | High efficiency base editors comprising Gam |
| GB201716590D0 (en) * | 2017-10-10 | 2017-11-22 | Univ Wageningen | Thermostable cas9 nucleases with reduced off-target activity |
| CN111757937A (zh) | 2017-10-16 | 2020-10-09 | 布罗德研究所股份有限公司 | 腺苷碱基编辑器的用途 |
| US20210395709A1 (en) * | 2018-10-18 | 2021-12-23 | Chan Zuckerberg Biohub, Inc. | Methods and compositions involving thermostable cas9 protein variants |
| WO2020089448A1 (en) | 2018-11-01 | 2020-05-07 | Keygene N.V. | Dual guide rna for crispr/cas genome editing in plants cells |
| RU2712497C1 (ru) * | 2018-11-26 | 2020-01-29 | Автономная некоммерческая образовательная организация высшего образования Сколковский институт науки и технологий | Средство разрезания ДНК на основе Cas9 белка из биотехнологически значимой бактерии Clostridium cellulolyticum |
| JP2022511633A (ja) | 2018-11-28 | 2022-02-01 | キージーン ナムローゼ フェンノートシャップ | エンドヌクレアーゼ保護による標的化濃縮 |
| DE112020001342T5 (de) | 2019-03-19 | 2022-01-13 | President and Fellows of Harvard College | Verfahren und Zusammensetzungen zum Editing von Nukleotidsequenzen |
| CN111979257B (zh) * | 2019-05-22 | 2023-10-13 | 上海凯赛生物技术股份有限公司 | 一种重组dna及其应用 |
| EP4069837A4 (en) * | 2019-12-10 | 2024-03-13 | Inscripta, Inc. | NEW MAD NUCLEASES |
| BR112022014777A2 (pt) * | 2020-01-27 | 2022-09-20 | Sherlock Biosciences Inc | Ensaios de detecção aprimorados |
| EP4146804A1 (en) | 2020-05-08 | 2023-03-15 | The Broad Institute Inc. | Methods and compositions for simultaneous editing of both strands of a target double-stranded nucleotide sequence |
| EP4251761A1 (en) | 2020-11-24 | 2023-10-04 | Keygene N.V. | Targeted enrichment using nanopore selective sequencing |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010054108A2 (en) * | 2008-11-06 | 2010-05-14 | University Of Georgia Research Foundation, Inc. | Cas6 polypeptides and methods of use |
| DK3401400T3 (da) * | 2012-05-25 | 2019-06-03 | Univ California | Fremgangsmåder og sammensætninger til rna-styret mål-dna-modifikation og til rna-styret transskriptionsmodulering |
| WO2016106239A1 (en) * | 2014-12-23 | 2016-06-30 | The Regents Of The University Of California | Methods and compositions for nucleic acid integration |
| GB201510296D0 (en) * | 2015-06-12 | 2015-07-29 | Univ Wageningen | Thermostable CAS9 nucleases |
| EP3555275A1 (en) * | 2016-12-14 | 2019-10-23 | Wageningen Universiteit | Thermostable cas9 nucleases |
-
2015
- 2015-06-12 GB GBGB1510296.5A patent/GB201510296D0/en not_active Ceased
-
2016
- 2016-06-06 BR BR112017026719A patent/BR112017026719A8/pt active Search and Examination
- 2016-06-06 JP JP2017564480A patent/JP7088528B2/ja active Active
- 2016-06-06 CA CA2989084A patent/CA2989084A1/en active Pending
- 2016-06-06 EP EP16727686.4A patent/EP3307883A1/en active Pending
- 2016-06-06 WO PCT/EP2016/062817 patent/WO2016198361A1/en active Application Filing
- 2016-06-06 CN CN201680046318.0A patent/CN107922931B/zh active Active
- 2016-06-06 EA EA201890032A patent/EA038500B1/ru unknown
- 2016-06-06 KR KR1020187001102A patent/KR20180019655A/ko not_active Application Discontinuation
- 2016-06-06 US US15/735,359 patent/US10731142B2/en active Active
- 2016-06-06 AU AU2016274452A patent/AU2016274452C1/en active Active
-
2017
- 2017-12-12 PH PH12017502281A patent/PH12017502281A1/en unknown
-
2020
- 2020-06-19 US US16/906,741 patent/US11802277B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CA2989084A1 (en) | 2016-12-15 |
| US10731142B2 (en) | 2020-08-04 |
| AU2016274452A1 (en) | 2018-01-25 |
| WO2016198361A1 (en) | 2016-12-15 |
| JP7088528B2 (ja) | 2022-06-21 |
| US11802277B2 (en) | 2023-10-31 |
| KR20180019655A (ko) | 2018-02-26 |
| AU2016274452C1 (en) | 2022-03-17 |
| AU2016274452B2 (en) | 2021-12-02 |
| BR112017026719A2 (pt) | 2018-08-28 |
| PH12017502281A1 (en) | 2018-06-11 |
| CN107922931A (zh) | 2018-04-17 |
| GB201510296D0 (en) | 2015-07-29 |
| US20180171314A1 (en) | 2018-06-21 |
| CN107922931B (zh) | 2022-07-26 |
| JP2018522544A (ja) | 2018-08-16 |
| EP3307883A1 (en) | 2018-04-18 |
| EA038500B1 (ru) | 2021-09-07 |
| EA201890032A1 (ru) | 2018-07-31 |
| US20200385697A1 (en) | 2020-12-10 |
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Free format text: DE ACORDO COM COMUNICADO PUBLICADO NA RPI 2526 DE 04/06/2019 FICAM DEVOLVIDOS DE 11/06/2019 ATE 14/06/2019 OS PRAZOS VENCIDOS NAS DATAS DIVULGADAS DENTRO DO COMUNICADO. |
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| B06U | Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette] | ||
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Owner name: WAGENINGEN UNIVERSITEIT (NL) |
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