BR112017015368A2 - método para a modificação precisa da planta através da expressão transiente do gene. - Google Patents

método para a modificação precisa da planta através da expressão transiente do gene.

Info

Publication number
BR112017015368A2
BR112017015368A2 BR112017015368A BR112017015368A BR112017015368A2 BR 112017015368 A2 BR112017015368 A2 BR 112017015368A2 BR 112017015368 A BR112017015368 A BR 112017015368A BR 112017015368 A BR112017015368 A BR 112017015368A BR 112017015368 A2 BR112017015368 A2 BR 112017015368A2
Authority
BR
Brazil
Prior art keywords
plant
gene
transient expression
specific
site
Prior art date
Application number
BR112017015368A
Other languages
English (en)
Inventor
Gao Caixia
Liu Jinxing
Zhang Kang
Wang Yanpeng
Zhang Yi
Original Assignee
Inst Genetics & Developmental Biology Cas
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Inst Genetics & Developmental Biology Cas filed Critical Inst Genetics & Developmental Biology Cas
Publication of BR112017015368A2 publication Critical patent/BR112017015368A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/008—Methods for regeneration to complete plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/04—Plant cells or tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/14—Plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Botany (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

a presente invenção refere-se a um método para a realização da modificações sítio-específica em uma planta, compreendendo as seguintes etapa: expressando de forma transiente uma nuclease específica de sequência para o fragmento alvo na célula ou no tecido da planta de interesse, onde a nuclease específica de sequência é específica para o sítio alvo e o sítio alvo é clivado pela nuclease, assim a modificação sítio-específica do sítio alvo é obtida através da reparação do dna da planta
BR112017015368A 2015-01-19 2016-01-19 método para a modificação precisa da planta através da expressão transiente do gene. BR112017015368A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201510025857 2015-01-19
PCT/CN2016/071352 WO2016116032A1 (en) 2015-01-19 2016-01-19 A method for precise modification of plant via transient gene expression

Publications (1)

Publication Number Publication Date
BR112017015368A2 true BR112017015368A2 (pt) 2018-01-16

Family

ID=56416438

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112017015368A BR112017015368A2 (pt) 2015-01-19 2016-01-19 método para a modificação precisa da planta através da expressão transiente do gene.

Country Status (11)

Country Link
US (1) US20180073035A1 (pt)
EP (1) EP3253879A4 (pt)
JP (2) JP7239266B2 (pt)
KR (1) KR102085189B1 (pt)
CN (1) CN105802991B (pt)
AR (1) AR103446A1 (pt)
AU (1) AU2016208913B2 (pt)
BR (1) BR112017015368A2 (pt)
CA (1) CA2973750A1 (pt)
EA (1) EA201791633A1 (pt)
WO (1) WO2016116032A1 (pt)

Families Citing this family (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2853829C (en) 2011-07-22 2023-09-26 President And Fellows Of Harvard College Evaluation and improvement of nuclease cleavage specificity
US20150044192A1 (en) 2013-08-09 2015-02-12 President And Fellows Of Harvard College Methods for identifying a target site of a cas9 nuclease
US9359599B2 (en) 2013-08-22 2016-06-07 President And Fellows Of Harvard College Engineered transcription activator-like effector (TALE) domains and uses thereof
US9526784B2 (en) 2013-09-06 2016-12-27 President And Fellows Of Harvard College Delivery system for functional nucleases
US9340800B2 (en) 2013-09-06 2016-05-17 President And Fellows Of Harvard College Extended DNA-sensing GRNAS
US9388430B2 (en) 2013-09-06 2016-07-12 President And Fellows Of Harvard College Cas9-recombinase fusion proteins and uses thereof
US20150166982A1 (en) 2013-12-12 2015-06-18 President And Fellows Of Harvard College Methods for correcting pi3k point mutations
US10077453B2 (en) 2014-07-30 2018-09-18 President And Fellows Of Harvard College CAS9 proteins including ligand-dependent inteins
EP4269577A3 (en) 2015-10-23 2024-01-17 President and Fellows of Harvard College Nucleobase editors and uses thereof
SG11201900907YA (en) 2016-08-03 2019-02-27 Harvard College Adenosine nucleobase editors and uses thereof
WO2018031683A1 (en) 2016-08-09 2018-02-15 President And Fellows Of Harvard College Programmable cas9-recombinase fusion proteins and uses thereof
CN106167787B (zh) * 2016-08-23 2020-01-14 浙江农林大学 一种光皮桦木质部原生质体制备及瞬时转化的方法
US11542509B2 (en) 2016-08-24 2023-01-03 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
US20190225974A1 (en) 2016-09-23 2019-07-25 BASF Agricultural Solutions Seed US LLC Targeted genome optimization in plants
WO2018067977A1 (en) 2016-10-07 2018-04-12 Kansas State University Research Foundation Genetic system for promoting recombination and gene transfer in wheat
CN110214180A (zh) 2016-10-14 2019-09-06 哈佛大学的校长及成员们 核碱基编辑器的aav递送
CN106755067A (zh) * 2016-12-05 2017-05-31 天津吉诺沃生物科技有限公司 通过瞬时表达对植物基因进行定点***的方法及获得的瞬时表达细胞、组织和突变植株
US10745677B2 (en) 2016-12-23 2020-08-18 President And Fellows Of Harvard College Editing of CCR5 receptor gene to protect against HIV infection
EP3592853A1 (en) 2017-03-09 2020-01-15 President and Fellows of Harvard College Suppression of pain by gene editing
EP3592777A1 (en) 2017-03-10 2020-01-15 President and Fellows of Harvard College Cytosine to guanine base editor
CN110914426A (zh) 2017-03-23 2020-03-24 哈佛大学的校长及成员们 包含核酸可编程dna结合蛋白的核碱基编辑器
EP3392339A1 (en) * 2017-04-18 2018-10-24 Rheinisch-Westfälische Technische Hochschule (RWTH) Aachen Improved genome editing in plant cells
WO2018209320A1 (en) 2017-05-12 2018-11-15 President And Fellows Of Harvard College Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation
GB201708662D0 (en) * 2017-05-31 2017-07-12 Tropic Biosciences Uk Ltd Compositions and methods for increasing shelf-life of banana
WO2019023680A1 (en) 2017-07-28 2019-01-31 President And Fellows Of Harvard College METHODS AND COMPOSITIONS FOR EVOLUTION OF BASIC EDITORS USING PHAGE-ASSISTED CONTINUOUS EVOLUTION (PACE)
US11319532B2 (en) 2017-08-30 2022-05-03 President And Fellows Of Harvard College High efficiency base editors comprising Gam
JP2021500036A (ja) 2017-10-16 2021-01-07 ザ ブロード インスティテュート, インコーポレーテッドThe Broad Institute, Inc. アデノシン塩基編集因子の使用
WO2019076355A1 (en) 2017-10-19 2019-04-25 Institute Of Genetics And Developmental Biology, Chinese Academy Of Sciences METHOD OF CONTROLLING GENE EXPRESSION
WO2019103034A1 (ja) * 2017-11-27 2019-05-31 国立研究開発法人理化学研究所 ゲノム編集植物の生産方法
US11739322B2 (en) 2018-02-01 2023-08-29 Institute Of Genetics And Developmental Biology, Chinese Academy Of Sciences Method for genome editing using a self-inactivating CRISPR nuclease
CN110396523B (zh) * 2018-04-23 2023-06-09 中国科学院分子植物科学卓越创新中心 一种重复片段介导的植物定点重组方法
WO2019227023A1 (en) 2018-05-24 2019-11-28 Monsanto Technology Llc Genome editing in plants
EP3800994A4 (en) * 2018-05-24 2022-03-09 Monsanto Technology LLC GENOME EDITING IN PLANTS
CN108753814B (zh) * 2018-06-11 2022-10-18 北京师范大学 一种加速物种突变的新育种方法
CN108823241A (zh) * 2018-07-17 2018-11-16 武汉伯远生物科技有限公司 一种自动分离基因编辑中t-dna标签的转基因方法
CN109402167A (zh) * 2018-12-07 2019-03-01 北京林业大学 一种在油松下胚轴中进行基因瞬时表达的方法
BR112021018607A2 (pt) 2019-03-19 2021-11-23 Massachusetts Inst Technology Métodos e composições para editar sequências de nucleotídeos
CN111850029B (zh) * 2019-04-08 2022-04-26 天津吉诺沃生物科技有限公司 一种获得非转基因多年生黑麦草突变体的方法
CN109971785A (zh) * 2019-04-18 2019-07-05 济宁学院 一种智能全基因组范围的植物基因功能鉴定***及方法
CN112779266A (zh) * 2019-11-06 2021-05-11 青岛清原化合物有限公司 在生物体内创制新基因的方法及应用
CN111110865A (zh) * 2019-11-27 2020-05-08 哈尔滨医科大学 一种腺相关病毒双重载体基因治疗***及其在治疗黏多糖贮积症ⅱ型中的应用
CN110714030A (zh) * 2019-12-03 2020-01-21 中国农业大学 一种长喙壳菌外源基因转化的方法
CN111575311A (zh) * 2020-04-15 2020-08-25 南京农业大学 一种基于基因枪介导的棉花基因编辑方法及应用
CN116096873A (zh) 2020-05-08 2023-05-09 布罗德研究所股份有限公司 同时编辑靶标双链核苷酸序列的两条链的方法和组合物

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102558309B (zh) * 2012-02-10 2014-09-17 浙江大学 一对转录激活子样效应因子核酸酶及其编码基因与应用
AU2013256240B2 (en) * 2012-05-02 2018-09-20 Corteva Agriscience Llc Targeted modification of malate dehydrogenase
US8697359B1 (en) * 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products
CN103382468B (zh) * 2013-07-04 2015-04-29 中国科学院遗传与发育生物学研究所 一种水稻基因组定点改造方法
CN103343120B (zh) * 2013-07-04 2015-03-04 中国科学院遗传与发育生物学研究所 一种小麦基因组定点改造方法
CN103667338B (zh) * 2013-11-28 2016-01-27 中国科学院遗传与发育生物学研究所 一种玉米基因组定点改造方法

Also Published As

Publication number Publication date
AU2016208913A1 (en) 2017-07-06
KR102085189B1 (ko) 2020-04-28
EP3253879A1 (en) 2017-12-13
EP3253879A4 (en) 2018-06-20
WO2016116032A1 (en) 2016-07-28
JP2021061868A (ja) 2021-04-22
KR20170098952A (ko) 2017-08-30
JP2018502590A (ja) 2018-02-01
US20180073035A1 (en) 2018-03-15
JP7239266B2 (ja) 2023-03-14
AU2016208913B2 (en) 2022-02-24
CA2973750A1 (en) 2016-07-28
CN105802991B (zh) 2021-06-29
CN105802991A (zh) 2016-07-27
EA201791633A1 (ru) 2018-03-30
AR103446A1 (es) 2017-05-10

Similar Documents

Publication Publication Date Title
BR112017015368A2 (pt) método para a modificação precisa da planta através da expressão transiente do gene.
BR112017015988A2 (pt) método para a modificação dirigida a sítio de planta inteira através da expressão transiente de gene
BR112013024337A2 (pt) locus de traço transgênico complexo em uma planta, planta ou semente, método para produzir em uma planta um locus de traço transgênico complexo e construto de expressão
BR112017007083A2 (pt) moléculas de ácido nucleico subunidade copi coatomer delta que confere resistência a pragas de coleóptero e hemípteros
MX2018004809A (es) Metodos y composiciones para la modificacion genomica exenta de marcadores.
BR112017016431A2 (pt) método para realização de modificação direcionada de sítio para genomas de plantas através de uso de materiais não herdados
BR112017018327A2 (pt) método para a expressão regulada de um complexo, planta ou célula vegetal, semente, método para modificação de uma sequência, método para alteração da expressão de pelo menos um polinucleotídeo
BR112017024754A2 (pt) método e construtos para a edição de ácido nucléico direcionada em plantas
BR112018009986A2 (pt) composições de indução haploide e métodos para sua utilização
BR112016010175A2 (pt) Repressor genético, seus usos, e composição farmacêutica
BR112016012870A8 (pt) composições e métodos para melhorar a produção de alface
UY33852A (es) ?moléculas de ácido nucleico que se dirigen a la proteína de unión a gtp pequeña de rho1 y que confieren resistencia a las plagas de coleópteros?.
BR112016026136A2 (pt) composições e métodos para aumentar o desenvolvimento e o rendimento vegetal
BR112015021210B8 (pt) Método para reduzir a expressão de um ácido nucleico alvo em uma planta e ácido nucleico recombinante
PH12016501191A1 (en) Ras opposite (rop) and related nucleic acid molecules that confer resistance to coleopteran and/or hemipteran pests
BR112017012407A2 (pt) supressão de rnai parental de genes de remodelagem de cromatina para controlar pragas coleópteras
NZ725789A (en) Dre4 nucleic acid molecules that confer resistance to coleopteran pests
CL2017000312A1 (es) Elección de microarn como diana para trastornos metabólicos
PH12016501170A1 (en) Rnapii-140 nucleic acid molecules that confer resistance to coleopteran pests
FI20145293A (fi) Leukamurskain, murskauslaitos ja menetelmä leukamurskaimen käyttämiseksi
CO2017009163A2 (es) Moléculas de ácido nucleico de arn polimerasa ii33 para el control de plagas de insectos
BR112017010955A2 (pt) composição para melhoramento da taxa na qual o fósforo de solo orgânico é convertido em ortofosfato, método para melhoramento da taxa na qual o fósforo de solo orgânico é convertido em ortofosfato, e método de aumento da taxa de crescimento de uma planta
BR112018007592A2 (pt) construto de polinucleotídeo, célula de planta de maís, planta de maís, método para produzir uma planta de maís
BR112017028448A2 (pt) análogos de cisteamina resistentes a ado e usos dos mesmos
BR112016030728A2 (pt) método para tratar ou prevenir uma inflamação induzida por bactérias em um indivíduo, composição imunogênica, composição farmacêutica, uso da composição imunogênica, método para produzir uma composição imunogênica, e, método para tratar ou prevenir uma doença

Legal Events

Date Code Title Description
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]