BR112016023304A2 - célula hospedeira recombinante engendrada para superexpressar proteínas auxiliares - Google Patents

célula hospedeira recombinante engendrada para superexpressar proteínas auxiliares

Info

Publication number
BR112016023304A2
BR112016023304A2 BR112016023304A BR112016023304A BR112016023304A2 BR 112016023304 A2 BR112016023304 A2 BR 112016023304A2 BR 112016023304 A BR112016023304 A BR 112016023304A BR 112016023304 A BR112016023304 A BR 112016023304A BR 112016023304 A2 BR112016023304 A2 BR 112016023304A2
Authority
BR
Brazil
Prior art keywords
host cell
recombinant host
cell engineered
helper proteins
protein
Prior art date
Application number
BR112016023304A
Other languages
English (en)
Inventor
Gasser Brigitte
Mattanovich Diethard
Buchetics Markus
Original Assignee
Boehringer Ingelheim Rcv Gmbh
Lonza Ag
Sandoz Ag
Vtu Tech Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Ingelheim Rcv Gmbh, Lonza Ag, Sandoz Ag, Vtu Tech Gmbh filed Critical Boehringer Ingelheim Rcv Gmbh
Publication of BR112016023304A2 publication Critical patent/BR112016023304A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

[001] a presente invenção está no campo de biotecnologia recombinante, em particular no campo de expressão de proteína. a invenção geralmente refere-se a um método de expressar uma proteína de interesse (poi) a partir de uma célula hospedeira. a invenção refere-se particularmente a melhorar a capacidade de uma célula hospedeira para expressar e/ou secretar uma proteína de interesse e ao uso da célula hospedeira para expressão de proteína. a invenção também refere-se à tecnologia de cultura celular, e mais especificamente a células de cultura para produzir moléculas desejadas para propósitos médicos ou produtos alimentícios.
BR112016023304A 2014-04-17 2015-04-16 célula hospedeira recombinante engendrada para superexpressar proteínas auxiliares BR112016023304A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP14165186 2014-04-17
PCT/EP2015/058242 WO2015158808A2 (en) 2014-04-17 2015-04-16 Recombinant host cell engineered to overexpress helper proteins

Publications (1)

Publication Number Publication Date
BR112016023304A2 true BR112016023304A2 (pt) 2017-10-17

Family

ID=50513080

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112016023304A BR112016023304A2 (pt) 2014-04-17 2015-04-16 célula hospedeira recombinante engendrada para superexpressar proteínas auxiliares

Country Status (11)

Country Link
US (1) US10865416B2 (pt)
EP (2) EP4311857A2 (pt)
JP (2) JP2017511148A (pt)
KR (1) KR102373954B1 (pt)
CN (1) CN106661541B (pt)
AU (1) AU2015248815B2 (pt)
BR (1) BR112016023304A2 (pt)
CA (1) CA2948369C (pt)
IL (1) IL248376B (pt)
SG (2) SG10201809146QA (pt)
WO (1) WO2015158808A2 (pt)

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WO2018178126A1 (en) * 2017-03-29 2018-10-04 Boehringer Ingelheim Rcv Gmbh & Co Kg Recombinant host cell with altered membrane lipid composition
CN109722445A (zh) * 2017-10-27 2019-05-07 成都必高生物科技有限公司 提升溶菌酶产量及活性的方法
AU2018368610A1 (en) * 2017-11-20 2020-05-28 Axxence Aromatic Gmbh Production of a flavour compound in a host cell
CN112166181B (zh) 2018-05-17 2024-03-08 保尔特纺织品公司 用于改善重组蛋白分泌的sec经修饰菌株
KR20210032972A (ko) * 2018-06-27 2021-03-25 베링거 인겔하임 에르체파우 게엠베하 운트 코 카게 전사인자를 사용하여 단백질 발현을 증가시키는 수단 및 방법
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CN109439639B (zh) * 2018-10-09 2021-07-20 天津科技大学 伏马毒素降解酶FumDPS及其基因和应用
CN109439640B (zh) * 2018-10-09 2021-07-20 天津科技大学 伏马毒素降解酶FumDSB及其基因和应用
CN111378585B (zh) 2018-12-28 2023-06-16 丰益(上海)生物技术研发中心有限公司 用于表达外源基因的毕赤酵母突变株
CN116904331A (zh) 2019-01-11 2023-10-20 龙沙有限公司 重组宿主细胞中碳源调节的蛋白的产生
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CN111349177B (zh) * 2020-03-13 2022-04-22 西北农林科技大学 一种融合抗菌肽cat的制备方法和应用
CN111607575B (zh) * 2020-04-07 2022-10-04 天津科技大学 一种转氨酶phta、制备方法和应用
CN111549007B (zh) * 2020-04-07 2022-10-04 天津科技大学 一种转氨酶tsta、制备方法和应用
CN113088533B (zh) * 2021-04-15 2023-03-24 华中科技大学 一种高效表达藤壶粘胶蛋白的酵母工程菌及其制备方法
CN113801801B (zh) * 2021-10-08 2023-03-07 枣庄市杰诺生物酶有限公司 一株高效生产碱性果胶酶的重组菌及其应用

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Also Published As

Publication number Publication date
JP2017511148A (ja) 2017-04-20
AU2015248815A1 (en) 2016-11-03
CN106661541A (zh) 2017-05-10
SG11201608084TA (en) 2016-10-28
CA2948369A1 (en) 2015-10-22
KR20170002453A (ko) 2017-01-06
EP4311857A2 (en) 2024-01-31
IL248376B (en) 2021-04-29
IL248376A0 (en) 2016-11-30
US10865416B2 (en) 2020-12-15
EP3132028B1 (en) 2023-10-25
JP7062633B2 (ja) 2022-05-06
WO2015158808A3 (en) 2016-01-28
CN106661541B (zh) 2021-09-03
US20170029827A1 (en) 2017-02-02
JP2020072696A (ja) 2020-05-14
CA2948369C (en) 2023-09-26
SG10201809146QA (en) 2018-11-29
EP3132028A2 (en) 2017-02-22
WO2015158808A2 (en) 2015-10-22
KR102373954B1 (ko) 2022-03-14
AU2015248815B2 (en) 2021-07-08

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