BR112016022447A2 - ressolubilização de cristais de proteína a ph baixo - Google Patents

ressolubilização de cristais de proteína a ph baixo

Info

Publication number
BR112016022447A2
BR112016022447A2 BR112016022447A BR112016022447A BR112016022447A2 BR 112016022447 A2 BR112016022447 A2 BR 112016022447A2 BR 112016022447 A BR112016022447 A BR 112016022447A BR 112016022447 A BR112016022447 A BR 112016022447A BR 112016022447 A2 BR112016022447 A2 BR 112016022447A2
Authority
BR
Brazil
Prior art keywords
resolubilization
crystals
protein
low protein
interest
Prior art date
Application number
BR112016022447A
Other languages
English (en)
Inventor
Peter Friis Esben
Martin Persson Jon
Erik Pedersen Poul
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Publication of BR112016022447A2 publication Critical patent/BR112016022447A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2462—Lysozyme (3.2.1.17)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01017—Lysozyme (3.2.1.17)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21062—Subtilisin (3.4.21.62)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag

Abstract

é revelado um método de purificação de um produto proteico preparado em um processo de fermentação onde a proteína de interesse está presente na forma sólida, cristalina ou amorfa no recipiente de fermentação, em que o ph do caldo de fermentação é ajustado a um ph baixo no qual a proteína de interesse se dissolve e pode ser eficientemente separada do insolúvel.
BR112016022447A 2014-03-28 2015-03-30 ressolubilização de cristais de proteína a ph baixo BR112016022447A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP14162436 2014-03-28
PCT/EP2015/056921 WO2015144936A1 (en) 2014-03-28 2015-03-30 Resolubilization of protein crystals at low ph

Publications (1)

Publication Number Publication Date
BR112016022447A2 true BR112016022447A2 (pt) 2017-10-10

Family

ID=50396954

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112016022447A BR112016022447A2 (pt) 2014-03-28 2015-03-30 ressolubilização de cristais de proteína a ph baixo

Country Status (7)

Country Link
US (1) US20170114091A1 (pt)
EP (1) EP3122760A1 (pt)
JP (2) JP6591998B2 (pt)
CN (1) CN106132995A (pt)
BR (1) BR112016022447A2 (pt)
MX (1) MX2016012260A (pt)
WO (1) WO2015144936A1 (pt)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017211803A1 (en) 2016-06-07 2017-12-14 Novozymes A/S Co-expression of heterologous polypeptides to increase yield
DK3606936T3 (da) * 2017-04-03 2022-01-10 Novozymes As Fremgangsmåde til indvinding
MX2021003035A (es) * 2018-09-17 2021-08-11 Dsm Ip Assets Bv Composiciones de alimento animal y usos de las mismas.
CA3121271A1 (en) 2018-11-28 2020-06-04 Novozymes A/S Modified filamentous fungal host cells
WO2021064068A1 (en) 2019-10-03 2021-04-08 Novozymes A/S Polypeptides comprising at least two carbohydrate binding domains
WO2022090555A1 (en) 2020-11-02 2022-05-05 Novozymes A/S Leader peptides and polynucleotides encoding the same
CN113388532B (zh) * 2021-06-16 2023-04-07 华东理工大学 一种用于生产天冬酰胺酶的重组里氏木霉及其构建方法和应用
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK6488D0 (da) * 1988-01-07 1988-01-07 Novo Industri As Enzymer
AU2742499A (en) * 1998-03-26 1999-10-18 Procter & Gamble Company, The Serine protease variants having amino acid substitutions
US20020192792A1 (en) * 2000-04-28 2002-12-19 Palle Schneider Laccase mutants
CA2419860A1 (en) * 2000-08-29 2002-03-07 Novozymes A/S Method for screening highly active proteases and inhibitors
NZ525735A (en) * 2000-11-22 2005-07-29 Univ Maryland Use of CLyA hemolysin for excretion of proteins
CN1473932A (zh) * 2003-06-30 2004-02-11 中山大学 一种重组石斑鱼腺苷酸环化酶激活多肽基因及其表达***、表达产物、生产方法和应用
CN1303207C (zh) * 2004-01-08 2007-03-07 中国药科大学 组织型纤溶酶原激活剂突变体的复性分离纯化方法
ES2368740T3 (es) * 2004-02-04 2011-11-21 Novo Nordisk A/S Métodos de replegamiento de glicosiltransferasas de mamífero.
WO2006002021A2 (en) * 2004-06-15 2006-01-05 Biotechnology Research And Development Corporation A highly active xylose reductase from neurospora crassa
DK2125865T3 (da) * 2007-03-15 2014-10-06 Novozymes As Opløsning af proteasekrystaller i fermenteringssuppe
US8568714B2 (en) * 2008-05-23 2013-10-29 The United States Of America, As Represented By The Secretary Of Agriculture Lys K endolysin is synergistic with lysostaphin against MRSA
AU2010328121A1 (en) * 2009-12-09 2012-06-07 Danisco Us Inc. Compositions and methods comprising protease variants
CN102234640B (zh) * 2010-04-22 2012-10-03 哈尔滨博翱生物医药技术开发有限公司 重组小分子泛素样修饰物蛋白酶及其制备方法和应用
CN102134575A (zh) * 2010-05-14 2011-07-27 昆明理工大学 大肠杆菌s-甲酰谷胱甘肽水解酶的原核表达载体及其构建方法和应用
ES2624531T3 (es) * 2011-11-25 2017-07-14 Novozymes A/S Polipéptidos que tienen actividad de lisozima y polinucleótidos que codifican los mismos
MX350713B (es) * 2012-02-17 2017-09-14 Novozymes As Variantes de subtilisina y polinucleotidos que las codifican.
CA2871042A1 (en) * 2012-04-20 2013-10-24 The Sun Products Corporation Liquid detergent compositions
WO2014055778A2 (en) * 2012-10-03 2014-04-10 Agrivida, Inc. Multiprotein expression cassettes

Also Published As

Publication number Publication date
JP2017508472A (ja) 2017-03-30
MX2016012260A (es) 2017-01-06
JP2019170384A (ja) 2019-10-10
EP3122760A1 (en) 2017-02-01
US20170114091A1 (en) 2017-04-27
JP6591998B2 (ja) 2019-10-16
WO2015144936A1 (en) 2015-10-01
CN106132995A (zh) 2016-11-16

Similar Documents

Publication Publication Date Title
BR112016022447A2 (pt) ressolubilização de cristais de proteína a ph baixo
PH12016502153A1 (en) Method for improving the production of cultured aquatic animals in combined rice-aquaculture systems
BR112017013748A2 (pt) método de produção & processamento de diaminas
BR112015032515A8 (pt) método para a produção de um açúcar líquido, método para a produção de um açúcar sólido, açúcar líquido, açúcar sólido e métodos para a produção de uma substância química
IT201700067289A1 (it) Processo e impianto combinato per la produzione di biogas da reflui organici, il recupero dei nutrienti e la pulizia del biogas prodotto
FR3025699B1 (fr) Extrait d'algue concentre, procede de preparation et ses utilisations en agriculture
BR112017000135A2 (pt) processo para a preparação de ácidos 4-alcóxi-3-hidroxipicolínico
BR112017004231A2 (pt) processo para produzir um precipitado estável enriquecido em ficobiliproteínas
MX2015006932A (es) Purificacion de mezclas acuosas derivadas de procesos de produccion de hidrocarburos.
PH12017502056A1 (en) A biostimulant formulation for improving plant growth and uses thereof
BR112013024257A2 (pt) método para reduzir ou prevenir a incrustação em um processo de produção de ácido fosfórico em processo a úmido
BR112018006390A2 (pt) método para fermentar um micro-organismo que produz um produto de proteína
BR112016025049A2 (pt) processo de coextração de líquido para produção de sacarose, xilo-oligossacarídeos e xilose a partir de matéria-prima
FR3028866B1 (fr) Procede, systeme et produit-programme d'ordinateur pour determiner la croissance de micro-organismes
TW201611886A (en) Kit for removing bacterial endotoxin in biological product, method thereof, and preparation method of biological product
PL409077A1 (pl) Sposób otrzymywania 4'-metoksy-α, β-dihydrochalkonu
WO2014031308A3 (en) Warewash machine with descaling/deliming system and method
BR112017016194A2 (pt) método
PH12020500175A1 (en) Method of recovering phosphoric acid from fermentation broth or fermentation waste liquid and reusing the same
BR112017010932A2 (pt) método para recuperar cristais de ácido succínico com o uso de tensoativos durante a cristalização e cristais resultantes
GB2545620A (en) System, method, and composition for enhancing solutions from bioreactors for processes including liquid fertilizer preparation and nutrient extraction
PL3227256T3 (pl) Sposób wytwarzania kwasu bursztynowego z bulionu fermentacyjnego z zastosowaniem nanoflltracji do oczyszczania zawracanego do obiegu ługu macierzystego
PH12018502693A1 (en) Crystal of amino acid salt of 3-hydroxyisovaleric acid and production method thereof
BR112017010403A2 (pt) método para a produção de praziquantel
MY194572A (en) Self-sufficient process for the production of biomass hydrolysate with reduced salt content

Legal Events

Date Code Title Description
B07D Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette]

Free format text: DE ACORDO COM O ARTIGO 229-C DA LEI NAO 10196/2001, QUE MODIFICOU A LEI NAO 9279/96, A CONCESSAO DA PATENTE ESTA CONDICIONADA A ANUAANCIA PRA VIA DA ANVISA. CONSIDERANDO A APROVAA AO DOS TERMOS DO PARECER NAO 337/PGF/EA/2010, BEM COMO A PORTARIA INTERMINISTERIAL NAO 1065 DE 24/05/2012, ENCAMINHA-SE O PRESENTE PEDIDO PARA AS PROVIDAANCIAS CABA-VEIS.

B07G Grant request does not fulfill article 229-c lpi (prior consent of anvisa) [chapter 7.7 patent gazette]
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE A 7A ANUIDADE.

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: EM VIRTUDE DO ARQUIVAMENTO PUBLICADO NA RPI 2664 DE 25-01-2022 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDO O ARQUIVAMENTO DO PEDIDO DE PATENTE, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.