BR112016018774A2 - microrganismos recombinantes para a melhoria da produção de mevalonato, isopreno, percursores de isoprenoide, isoprenoides e produtos derivados de acetil-coa - Google Patents

microrganismos recombinantes para a melhoria da produção de mevalonato, isopreno, percursores de isoprenoide, isoprenoides e produtos derivados de acetil-coa

Info

Publication number
BR112016018774A2
BR112016018774A2 BR112016018774A BR112016018774A BR112016018774A2 BR 112016018774 A2 BR112016018774 A2 BR 112016018774A2 BR 112016018774 A BR112016018774 A BR 112016018774A BR 112016018774 A BR112016018774 A BR 112016018774A BR 112016018774 A2 BR112016018774 A2 BR 112016018774A2
Authority
BR
Brazil
Prior art keywords
isoprene
isoprenes
mevalonate
acetyl
production
Prior art date
Application number
BR112016018774A
Other languages
English (en)
Inventor
J Wolfe Alan
H Wells Derek
S Dasika Madhukar
A Cervin Marguerite
C Miller Michael
Original Assignee
Danisco Us Inc
Goodyear Tire & Rubber
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco Us Inc, Goodyear Tire & Rubber filed Critical Danisco Us Inc
Publication of BR112016018774A2 publication Critical patent/BR112016018774A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/007—Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42—Hydroxy-carboxylic acids

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

a invenção apresenta composições e métodos para o aumento da produção de mevalonato, isopreno, moléculas precursoras de isoprenoides, isoprenoides, e/ou produtos derivados de acetil-coa em microrganismos recombinantes por manipulação dos microrganismos para compreender uma ou mais proteínas de acetilação de modo a que a expressão e/ou atividade de uma ou mais proteínas de acetilação é modulada.
BR112016018774A 2014-02-20 2015-02-20 microrganismos recombinantes para a melhoria da produção de mevalonato, isopreno, percursores de isoprenoide, isoprenoides e produtos derivados de acetil-coa BR112016018774A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201461942546P 2014-02-20 2014-02-20
PCT/US2015/016954 WO2015127305A2 (en) 2014-02-20 2015-02-20 Recombinant microorganisms for the enhanced production of mevalonate, isoprene, isoprenoid precursors, isoprenoids, and acetyl-coa-derived products

Publications (1)

Publication Number Publication Date
BR112016018774A2 true BR112016018774A2 (pt) 2017-10-10

Family

ID=52633654

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112016018774A BR112016018774A2 (pt) 2014-02-20 2015-02-20 microrganismos recombinantes para a melhoria da produção de mevalonato, isopreno, percursores de isoprenoide, isoprenoides e produtos derivados de acetil-coa

Country Status (6)

Country Link
US (2) US10648004B2 (pt)
EP (1) EP3107993B1 (pt)
JP (1) JP6744819B2 (pt)
BR (1) BR112016018774A2 (pt)
DK (1) DK3107993T3 (pt)
WO (1) WO2015127305A2 (pt)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112016018774A2 (pt) 2014-02-20 2017-10-10 Danisco Us Inc microrganismos recombinantes para a melhoria da produção de mevalonato, isopreno, percursores de isoprenoide, isoprenoides e produtos derivados de acetil-coa
CA3047268A1 (en) * 2016-12-16 2018-06-21 Danisco Us Inc. Bifunctional phosphoketolase-phosphotransacetylase fusion polypeptides
EP3570682A4 (en) * 2017-01-17 2021-03-03 White Dog Labs, Inc. PROTEINIC BIOMASS PREPARATION WITH A NON-NATIVE ORGANISM OF THE CLOSTRIDIA CLASS
WO2019113387A1 (en) 2017-12-07 2019-06-13 Zymergen Inc. Engineered biosynthetic pathways for production of (6e)-8-hydroxygeraniol by fermentation
EP3728212A1 (en) 2017-12-21 2020-10-28 Zymergen Inc. Nepetalactol oxidoreductases, nepetalactol synthases, and microbes capable of producing nepetalactone
WO2019165418A1 (en) * 2018-02-26 2019-08-29 William Marsh Rice University Improved acetyl-coa-derived biosynthesis
CN109486720B (zh) * 2018-12-18 2020-08-04 江南大学 一种产l-木酮糖的枯草芽孢杆菌及其应用
JP6917434B2 (ja) * 2019-10-25 2021-08-11 長瀬産業株式会社 好気性菌の代謝酵素破壊株およびその培養法
CN111607546B (zh) * 2020-04-30 2022-08-09 中国科学院青岛生物能源与过程研究所 一种高产法尼烯的基因工程菌及其构建方法与应用
EP4370690A1 (en) * 2021-07-12 2024-05-22 Danisco Us Inc Recombinant yeast cell
KR102572850B1 (ko) * 2022-07-11 2023-08-31 대상 주식회사 L-글루탐산을 생산하는 코리네박테리움 속 변이 미생물 및 이를 이용한 l-글루탐산의 생산 방법

Family Cites Families (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19629568C1 (de) 1996-07-15 1998-01-08 Fraunhofer Ges Forschung Verfahren zur Herstellung von Isopren
US7172886B2 (en) 2001-12-06 2007-02-06 The Regents Of The University Of California Biosynthesis of isopentenyl pyrophosphate
WO2003095651A1 (fr) 2002-05-10 2003-11-20 Kyowa Hakko Kogyo Co., Ltd. Procede de production d'acide mevalonique
US7531337B2 (en) * 2002-06-07 2009-05-12 Wisconsin Alumni Research Foundation Method of controlling acetylation of metabolic enzymes
AU2003287028B2 (en) 2002-10-04 2008-09-04 E.I. Du Pont De Nemours And Company Process for the biological production of 1,3-propanediol with high yield
RU2004124226A (ru) 2004-08-10 2006-01-27 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) (RU) Использование фосфокетолазы для продукции полезных метаболитов
WO2007024718A2 (en) * 2005-08-19 2007-03-01 The Regents Of The University Of California Genetically modified host cells and use of same for producing isoprenoid compounds
MX284139B (es) 2006-05-26 2011-02-18 Amyris Biotechnologies Inc Produccion de isoprenoides.
JP4986547B2 (ja) 2006-09-04 2012-07-25 株式会社Ａｄｅｋａ 新規なアセトアセチルＣｏＡ合成酵素、それをコードするＤＮＡ配列、当該酵素の製造方法および当該酵素を利用したメバロン酸の製造方法
BRPI0823506A8 (pt) 2007-12-13 2018-05-15 Danisco Us Inc Genencor Div composições e métodos para a produção de isoprene
WO2009100231A2 (en) 2008-02-06 2009-08-13 The Regents Of The University Of California Short chain volatile isoprene hydrocarbon production using the mevalonic acid pathway in genetically engineered yeast and fungi
MX2010011068A (es) * 2008-04-08 2010-11-04 Amyris Biotechnologies Inc Expresion de secuencias heterologas.
ZA201007564B (en) 2008-04-23 2012-01-25 Goodyear Tire & Rubber Isoprene synthase variants for improved microbial production of isoprene
US7632995B2 (en) 2008-05-09 2009-12-15 Monsanto Technology Llc Plants and seeds of hybrid corn variety CH852179
JP2011526790A (ja) 2008-07-02 2011-10-20 ダニスコ・ユーエス・インク 分離された条件下および／または安全操業条件下における、ｃ５炭化水素を含まないイソプレン組成物及びその製造方法
HU3516U (en) 2008-07-31 2008-11-28 Zoltan Kuthi Anti-theft protection of draining for fuel tanks
CA2737195A1 (en) 2008-09-15 2010-03-18 The Goodyear Tire & Rubber Company Conversion of prenyl derivatives to isoprene
WO2010031062A1 (en) 2008-09-15 2010-03-18 Danisco Us Inc. Increased isoprene production using the archaeal lower mevalonate pathway
US8470581B2 (en) 2008-09-15 2013-06-25 Danisco Us Inc. Reduction of carbon dioxide emission during isoprene production by fermentation
EP2344629B1 (en) 2008-09-15 2018-01-24 Danisco US Inc. Systems using cell culture for production of isoprene
SG169640A1 (en) 2008-09-15 2011-04-29 Danisco Us Inc Increased isoprene production using mevalonate kinase and isoprene synthase
WO2010078457A2 (en) 2008-12-30 2010-07-08 Danisco Us Inc. Methods of producing isoprene and a co-product
US20100297749A1 (en) 2009-04-21 2010-11-25 Sapphire Energy, Inc. Methods and systems for biofuel production
US8518686B2 (en) 2009-04-23 2013-08-27 Danisco Us Inc. Three-dimensional structure of isoprene synthase and its use thereof for generating variants
JP4760951B2 (ja) 2009-05-08 2011-08-31 トヨタ自動車株式会社 ブタノール生産能を有する組換え微生物及びブタノールの製造方法
TW201412988A (zh) 2009-06-17 2014-04-01 Danisco Us Inc 使用ｄｘｐ及ｍｖａ途徑之改良之異戊二烯製造
TWI434921B (zh) 2009-06-17 2014-04-21 Danisco Us Inc 從生物異戊二烯組合物製造燃料成分之方法及系統
IN2012DN01694A (pt) 2009-09-15 2015-06-05 Sapphire Energy Inc
JP5643838B2 (ja) 2009-12-18 2014-12-17 ダニスコ・ユーエス・インク 再生可能資源からのイソプレンの精製
EP2857518A3 (en) 2009-12-23 2015-04-22 Danisco US Inc. Compositions and methods of PGL for the increased production of isoprene
CA2844064A1 (en) 2011-08-04 2013-02-07 Danisco Us Inc. Production of isoprene, isoprenoid precursors, and isoprenoids using acetoacetyl-coa synthase
DK3339426T3 (da) * 2011-10-07 2022-09-12 Danisco Us Inc Anvendelse af phosphoketolase i fremstillingen af mevalonat, isoprenoid-udgangsstoffer og isopren
US8895277B2 (en) * 2012-05-02 2014-11-25 Danisco Us Inc. Legume isoprene synthase for production of isoprene
WO2014015210A2 (en) 2012-07-20 2014-01-23 Glycos Biotechnologies, Inc. Microorganisms and processes for the conversion of glycerol to isoprene
BR112016018774A2 (pt) 2014-02-20 2017-10-10 Danisco Us Inc microrganismos recombinantes para a melhoria da produção de mevalonato, isopreno, percursores de isoprenoide, isoprenoides e produtos derivados de acetil-coa

Also Published As

Publication number Publication date
US11685937B2 (en) 2023-06-27
US20170159074A1 (en) 2017-06-08
EP3107993A2 (en) 2016-12-28
JP6744819B2 (ja) 2020-08-19
US20200340017A1 (en) 2020-10-29
DK3107993T3 (da) 2020-11-23
WO2015127305A2 (en) 2015-08-27
JP2017506078A (ja) 2017-03-02
EP3107993B1 (en) 2020-08-26
US10648004B2 (en) 2020-05-12
WO2015127305A3 (en) 2015-12-03

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B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]
B12B Appeal against refusal [chapter 12.2 patent gazette]