BR112015015107A2 - método para remover contaminantes bacterianos de solução sacarificada e sistema de fermentação - Google Patents

método para remover contaminantes bacterianos de solução sacarificada e sistema de fermentação

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Publication number
BR112015015107A2
BR112015015107A2 BR112015015107A BR112015015107A BR112015015107A2 BR 112015015107 A2 BR112015015107 A2 BR 112015015107A2 BR 112015015107 A BR112015015107 A BR 112015015107A BR 112015015107 A BR112015015107 A BR 112015015107A BR 112015015107 A2 BR112015015107 A2 BR 112015015107A2
Authority
BR
Brazil
Prior art keywords
saccharified solution
bacterial contaminants
fermentation
fermentation system
yeast
Prior art date
Application number
BR112015015107A
Other languages
English (en)
Other versions
BR112015015107B1 (pt
Inventor
Masamoto Manabu
Izumi Noriaki
Konishi Satoshi
Original Assignee
Kawasaki Heavy Ind Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kawasaki Heavy Ind Ltd filed Critical Kawasaki Heavy Ind Ltd
Publication of BR112015015107A2 publication Critical patent/BR112015015107A2/pt
Publication of BR112015015107B1 publication Critical patent/BR112015015107B1/pt

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/14Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/12Bioreactors or fermenters specially adapted for specific uses for producing fuels or solvents
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/18External loop; Means for reintroduction of fermented biomass or liquid percolate
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/02Separating microorganisms from the culture medium; Concentration of biomass
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/12Purification
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D21/00Separation of suspended solid particles from liquids by sedimentation
    • B01D21/26Separation of sediment aided by centrifugal force or centripetal force
    • B01D21/262Separation of sediment aided by centrifugal force or centripetal force by using a centrifuge
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D2311/00Details relating to membrane separation process operations and control
    • B01D2311/26Further operations combined with membrane separation processes
    • B01D2311/2688Biological processes
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D61/00Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/14Ultrafiltration; Microfiltration
    • B01D61/147Microfiltration
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02Separating microorganisms from their culture media
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • C12N1/16Yeasts; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06Ethanol, i.e. non-beverage
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels, e.g. bio-diesel

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Water Supply & Treatment (AREA)

Abstract

resumo patente de invenção: "método para remover contaminantes bacterianos de solução sacarificada e sistema de fermentação". a presente invenção refere-se a um método para remover contaminantes bacterianos de uma solução sacarificada, por meio do qual se torna possível evitar a proliferação de bactérias em um vaso de fermentação sem exigir o uso de um antibiótico na fermentação alcoólica de uma solução sacarificada de biomassa com o uso de levedura convencional; e um sistema de fermentação que é adequado para a implantação do método para remover contaminantes bacterianos de uma solução sacarificada. no método de remoção de contaminantes bacterianos e no sistema de fermentação de acordo a presente invenção, a separação grosseira, que é um procedimento para extrair uma solução sacarificada (uma solução de fermentação) de um tanque de fermentação e, então, coletar a levedura da solução sacarificada, é executada. posteriormente, a separação fina, que é um procedimento para remover os contaminantes bacterianos da solução sacarificada da qual a levedura foi separada, é executada. dessa maneira, somente os contaminantes bacterianos podem ser removidos da solução sacarificada enquanto a levedura é coletada.
BR112015015107-8A 2012-12-25 2013-12-03 método para produzir etanol a partir de uma solução sacarificada BR112015015107B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2012280900A JP6097553B2 (ja) 2012-12-25 2012-12-25 糖化液の雑菌除去方法及び発酵システム
JP2012-280900 2012-12-25
PCT/JP2013/007085 WO2014103183A1 (ja) 2012-12-25 2013-12-03 糖化液の雑菌除去方法及び発酵システム

Publications (2)

Publication Number Publication Date
BR112015015107A2 true BR112015015107A2 (pt) 2017-07-11
BR112015015107B1 BR112015015107B1 (pt) 2020-12-08

Family

ID=50985826

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112015015107-8A BR112015015107B1 (pt) 2012-12-25 2013-12-03 método para produzir etanol a partir de uma solução sacarificada

Country Status (5)

Country Link
US (1) US9481860B2 (pt)
JP (1) JP6097553B2 (pt)
CN (2) CN103894065B (pt)
BR (1) BR112015015107B1 (pt)
WO (1) WO2014103183A1 (pt)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6097553B2 (ja) * 2012-12-25 2017-03-15 川崎重工業株式会社 糖化液の雑菌除去方法及び発酵システム
AP2016009637A0 (en) * 2014-07-21 2016-12-31 Xyleco Inc Processing biomass
JP2018164864A (ja) * 2017-03-28 2018-10-25 家田貿易株式会社 懸濁液の濃縮システム
JP2019106954A (ja) * 2017-12-19 2019-07-04 川崎重工業株式会社 セルロース系バイオマスを原料とする酵素法によるバイオエタノール製造方法
JP7308067B2 (ja) * 2019-04-17 2023-07-13 株式会社日立製作所 細胞培養精製装置及び細胞培養精製方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4460687A (en) * 1981-03-23 1984-07-17 Alfa Laval Ab Fermentation method
SE449876B (sv) * 1984-12-07 1987-05-25 Nobel Chematur Ab Forfarande for framstellning av etanol med ett ytterligare centrifugalsepareringssteg, placerat antingen fore eller efter det primera destillationssteget
CN1687439A (zh) * 2005-04-07 2005-10-26 江苏诚意药业有限公司 微生物发酵法生产腺苷的生产工艺
CN100396765C (zh) * 2005-04-25 2008-06-25 陈长寿 一种以柚为原料的果酒生产工艺
JP4854568B2 (ja) * 2007-03-30 2012-01-18 三井造船株式会社 アルコール生産方法
AU2008291588A1 (en) * 2007-08-27 2009-03-05 Iogen Energy Corporation Method for the production of a fermentation product from a pretreated lignocellulosic feedstock
JP5187823B2 (ja) 2007-12-14 2013-04-24 独立行政法人農業・食品産業技術総合研究機構 アルコール発酵に適した新規酵母及びそれを用いたアルコール製造方法
CN101392273B (zh) 2008-11-10 2013-02-06 南京工业大学 一种乳酸的清洁生产工艺
WO2010110448A1 (ja) * 2009-03-27 2010-09-30 三井造船株式会社 糖原料からのアルコール連続発酵方法及び装置
JP2011092041A (ja) 2009-10-28 2011-05-12 Kansai Chemical Engineering Co Ltd エタノール生産微生物の連続培養発酵装置
JP6097553B2 (ja) 2012-12-25 2017-03-15 川崎重工業株式会社 糖化液の雑菌除去方法及び発酵システム

Also Published As

Publication number Publication date
US9481860B2 (en) 2016-11-01
BR112015015107B1 (pt) 2020-12-08
JP6097553B2 (ja) 2017-03-15
WO2014103183A1 (ja) 2014-07-03
JP2014124101A (ja) 2014-07-07
US20150337253A1 (en) 2015-11-26
CN103894065A (zh) 2014-07-02
CN203741334U (zh) 2014-07-30
CN103894065B (zh) 2017-01-11

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B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
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B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
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