BR112015010134A2 - Processo para consumo de acetato durante a fermentação etanólica de matérias-primas celulósicas - Google Patents
Processo para consumo de acetato durante a fermentação etanólica de matérias-primas celulósicasInfo
- Publication number
- BR112015010134A2 BR112015010134A2 BR112015010134A BR112015010134A BR112015010134A2 BR 112015010134 A2 BR112015010134 A2 BR 112015010134A2 BR 112015010134 A BR112015010134 A BR 112015010134A BR 112015010134 A BR112015010134 A BR 112015010134A BR 112015010134 A2 BR112015010134 A2 BR 112015010134A2
- Authority
- BR
- Brazil
- Prior art keywords
- acetate
- conversion
- ethanol
- biomass
- isopropanol
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
- C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
- C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
- C12Y101/01175—D-Xylose 1-dehydrogenase (1.1.1.175)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
- C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
- C12Y101/01307—D-Xylose reductase (1.1.1.307)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
- C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
- C12Y102/0101—Acetaldehyde dehydrogenase (acetylating) (1.2.1.10)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Mycology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
MICRO-ORGANISMOS RECOMBINANTES, VIA METABÓLICA ENGENHEIRADA E PROCESSOS PARA AUMENTAR A CAPTAÇÃO DE ACETATO PARTINDO DE UMA BIOMASSA E PARA CONVERSÃO DE BIOMASSA EM ETANOL, ACETONA OU ISOPROPANOL. A presente invenção refere-se a novas vias metabólicas para desintoxicar acetato derivado da biomassa através da conversão metabólica em etanol, acetona ou isopropanol. Mais especificamente, a invenção refere-se a um micro-organismo recombinante que compreende uma ou mais enzimas nativas e/ou heterólogas que funcionam em uma ou mais primeiras vias metabólicas engenheiradas para atingir: (1) conversão de acetato em etanol; (2) conversão de acetato em acetona; ou (3) conversão de acetato em isopropanol; e uma ou mais enzimas nativas e/ou heterólogas que funcionam em uma ou mais segundas vias metabólicas engenheiradas para produzir um doador de elétron utilizado na conversão de acetato em compostos menos inibidores; em que a uma ou mais enzimas nativas e/ou heterólogas são ativadas, reguladas para mais ou reguladas para menos.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201261724831P | 2012-11-09 | 2012-11-09 | |
US201361793716P | 2013-03-15 | 2013-03-15 | |
PCT/US2013/069266 WO2014074895A2 (en) | 2012-11-09 | 2013-11-08 | Method for acetate consumption during ethanolic fermentation of cellulosic feedstocks |
Publications (1)
Publication Number | Publication Date |
---|---|
BR112015010134A2 true BR112015010134A2 (pt) | 2017-08-22 |
Family
ID=49880925
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112015010134A BR112015010134A2 (pt) | 2012-11-09 | 2013-11-08 | Processo para consumo de acetato durante a fermentação etanólica de matérias-primas celulósicas |
Country Status (6)
Country | Link |
---|---|
US (4) | US20140256011A1 (pt) |
EP (2) | EP2917343A2 (pt) |
CN (1) | CN104903441A (pt) |
BR (1) | BR112015010134A2 (pt) |
CA (1) | CA2889890C (pt) |
WO (1) | WO2014074895A2 (pt) |
Families Citing this family (31)
Publication number | Priority date | Publication date | Assignee | Title |
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EP2292741A1 (en) * | 2009-08-26 | 2011-03-09 | OrganoBalance GmbH | Genetically modified organisms for the production of lipids |
CA2798452C (en) * | 2010-05-05 | 2019-09-03 | Mascoma Corporation | Detoxification of biomass derived acetate via metabolic conversion to ethanol, acetone, isopropanol, or ethyl acetate |
CN104024419A (zh) | 2011-10-24 | 2014-09-03 | 丰田自动车株式会社 | 使用了重组酵母的乙醇的制造方法 |
US8707454B1 (en) | 2012-07-16 | 2014-04-22 | Wickr Inc. | Multi party messaging |
BR112015019777B1 (pt) * | 2013-02-27 | 2022-12-06 | Toyota Jidosha Kabushiki Kaisha | Método para produzir etanol com o uso de saccharomyces cerevisiae recombinante |
WO2014160402A1 (en) * | 2013-03-14 | 2014-10-02 | Mascoma Corporation | Co-conversion of carbohydrates to fermentation products in a single fermentation step |
US10129260B1 (en) | 2013-06-25 | 2018-11-13 | Wickr Inc. | Mutual privacy management |
US10567349B2 (en) | 2013-06-25 | 2020-02-18 | Wickr Inc. | Secure time-to-live |
WO2015023989A1 (en) * | 2013-08-15 | 2015-02-19 | Lallemand Hungary Liquidity Management Llc | Methods for the improvement of product yield and production in a microorganism through glycerol recycling |
AR097480A1 (es) * | 2013-08-29 | 2016-03-16 | Dsm Ip Assets Bv | Células de levadura convertidoras de glicerol y ácido acético con una conversión de ácido acético mejorada |
WO2015108777A1 (en) * | 2014-01-16 | 2015-07-23 | Papoutsakis Eleftherios T | Synthetic methylotrophy to liquid fuels and chemicals |
US9698976B1 (en) | 2014-02-24 | 2017-07-04 | Wickr Inc. | Key management and dynamic perfect forward secrecy |
US20170130210A1 (en) * | 2014-06-09 | 2017-05-11 | Massachusetts Institute Of Technology | Dynamic knockdown of central metabolism for redirecting glucose-6-phosphate fluxes |
EP3169768B1 (de) * | 2014-07-14 | 2018-09-26 | Jacobs University Bremen GmbH | Gentechnisch veränderte hefe mit verbessertem glycerol-katabolismus |
CN104357344A (zh) * | 2014-11-19 | 2015-02-18 | 江南大学 | 一种强化解脂亚洛酵母合成α-酮戊二酸的方法 |
WO2016162712A1 (en) * | 2015-04-07 | 2016-10-13 | Metabolic Explorer | Modified microorganism for the optimized production of 2,4-dihydroxyburyrate |
US10291607B1 (en) | 2016-02-02 | 2019-05-14 | Wickr Inc. | Providing real-time events to applications |
US9591479B1 (en) | 2016-04-14 | 2017-03-07 | Wickr Inc. | Secure telecommunications |
JP6447583B2 (ja) * | 2016-06-24 | 2019-01-09 | トヨタ自動車株式会社 | 組換え酵母、及びそれを用いたエタノールの製造方法 |
JP6879111B2 (ja) * | 2017-08-02 | 2021-06-02 | トヨタ自動車株式会社 | 組換え酵母及びこれを用いたエタノールの製造方法 |
CA3091962A1 (en) | 2017-09-19 | 2019-03-28 | Lallemand Hungary Liquidity Management Llc | Acetate toxicity tolerance in recombinant microbial host cells |
US11036283B2 (en) * | 2018-01-18 | 2021-06-15 | Richard J. Tett | Navigation controller |
US20210087571A1 (en) * | 2018-02-26 | 2021-03-25 | William Marsh Rice University | Acetyl-coa-derived biosynthesis |
CN108588166B (zh) * | 2018-06-13 | 2021-11-16 | 杭州富阳佳畅机械有限公司 | 利用玉米秸秆为主要原料发酵生产生物丁醇和生物乙醇的方法 |
JP7078900B2 (ja) | 2018-10-05 | 2022-06-01 | トヨタ自動車株式会社 | 形質転換酵母及びこれを用いたエタノールの製造方法 |
BR112021009163A2 (pt) * | 2018-11-13 | 2021-08-17 | Lallemand Hungary Liquidity Management Llc. | combinações sinergísticas de bactéria e levedura |
US20200208160A1 (en) | 2018-12-28 | 2020-07-02 | Braskem S.A. | Modulation of carbon flux through the meg and c3 pathways for the improved production of monoethylene glycol and c3 compounds |
CN109666683B (zh) * | 2019-02-27 | 2021-10-29 | 昆明理工大学 | 乙酰辅酶A乙酰转移酶基因RKAcaT2及其应用 |
CN115975832B (zh) * | 2020-03-11 | 2024-03-22 | 大连理工大学 | 甲酸脱氢酶在提高微生物发酵菌株对纤维素水解液中甲酸和乙酸抗性中的应用 |
EP4146814A1 (en) * | 2020-05-05 | 2023-03-15 | Stellenbosch University | Method of modifying a yeast strain, modified yeast strains obtained thereby and uses thereof |
WO2022245340A1 (en) * | 2021-05-18 | 2022-11-24 | Zymergen Inc. | Engineered biosynthetic pathways for production of deoxyhydrochorismic acid by fermentation |
Family Cites Families (14)
Publication number | Priority date | Publication date | Assignee | Title |
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WO2004085627A1 (en) * | 2003-03-26 | 2004-10-07 | Forskarpatent I Syd Ab | New saccharomyces cerevisiae strains utilizing xylose |
WO2006009434A1 (en) | 2004-07-16 | 2006-01-26 | Technische Universiteit Delft | Metabolic engineering of xylose fermenting eukaryotic cells |
KR20100087695A (ko) * | 2007-10-12 | 2010-08-05 | 더 리전트 오브 더 유니버시티 오브 캘리포니아 | 이소프로판올을 생산하도록 조작된 미생물 |
WO2010062707A1 (en) * | 2008-10-30 | 2010-06-03 | Joule Unlimited, Inc. | Methods and compositions for producing carbon-based products of interest in micro-organisms |
WO2010056805A2 (en) * | 2008-11-12 | 2010-05-20 | Mascoma Corporation | Gene knockout mesophilic and thermophilic organisms, and methods of use thereof |
DK2361311T3 (en) | 2008-11-21 | 2017-04-24 | Lallemand Hungary Liquidity Man Llc | MAKE EXPRESSIVE CELLULASES FOR SIMULTANEOUS INSURANCE AND ACTION BY CELLULOSE |
EP2277989A1 (en) | 2009-07-24 | 2011-01-26 | Technische Universiteit Delft | Fermentative glycerol-free ethanol production |
CA2798452C (en) * | 2010-05-05 | 2019-09-03 | Mascoma Corporation | Detoxification of biomass derived acetate via metabolic conversion to ethanol, acetone, isopropanol, or ethyl acetate |
WO2011149353A1 (en) | 2010-05-27 | 2011-12-01 | C5 Yeast Company B.V. | Yeast strains engineered to produce ethanol from acetic acid and glycerol |
DK2663645T3 (da) * | 2010-11-18 | 2015-03-23 | Dsm Ip Assets Bv | Gærstammer, der er modificeret til produktion af ethanol fra glycerol |
BR122019017739B1 (pt) | 2011-04-05 | 2021-06-22 | Lallemand Hungary Liquidity Management Llc | Micro-organismo recombinante compreendendo uma deleção de enzimas nativas que atuam para produzir glicerol e/ou regular a síntese de glicerol e vias metabólicas sintéticas para converter uma fonte de carboidrato a etanol |
EP2785827A4 (en) * | 2011-11-29 | 2015-09-23 | Codexis Inc | OVEREXPRESSION OF GENES ENHANCING YEAST FERMENTATION USING CELLULOSIC SUBSTRATES |
KR20140099251A (ko) * | 2011-11-30 | 2014-08-11 | 디에스엠 아이피 어셋츠 비.브이. | 아세트산 및 글리세롤로부터 에탄올을 생성하도록 합성된 이스트 스트레인 |
US11698108B2 (en) | 2020-08-13 | 2023-07-11 | Steering Solutions Ip Holding Corporation | Tripot joint with assembly and disassembly ring |
-
2013
- 2013-11-08 US US14/075,846 patent/US20140256011A1/en not_active Abandoned
- 2013-11-08 EP EP13812217.1A patent/EP2917343A2/en not_active Withdrawn
- 2013-11-08 CN CN201380069842.6A patent/CN104903441A/zh active Pending
- 2013-11-08 EP EP18171345.4A patent/EP3498829A1/en active Pending
- 2013-11-08 CA CA2889890A patent/CA2889890C/en active Active
- 2013-11-08 BR BR112015010134A patent/BR112015010134A2/pt not_active Application Discontinuation
- 2013-11-08 WO PCT/US2013/069266 patent/WO2014074895A2/en active Application Filing
-
2016
- 2016-05-10 US US15/150,534 patent/US20160265005A1/en not_active Abandoned
-
2019
- 2019-12-04 US US16/703,399 patent/US20200095613A1/en not_active Abandoned
-
2021
- 2021-05-13 US US17/319,223 patent/US20210277427A1/en active Pending
Also Published As
Publication number | Publication date |
---|---|
CA2889890C (en) | 2023-03-28 |
CA2889890A1 (en) | 2014-05-15 |
CN104903441A (zh) | 2015-09-09 |
EP3498829A1 (en) | 2019-06-19 |
WO2014074895A2 (en) | 2014-05-15 |
US20160265005A1 (en) | 2016-09-15 |
EP2917343A2 (en) | 2015-09-16 |
US20210277427A1 (en) | 2021-09-09 |
US20140256011A1 (en) | 2014-09-11 |
US20200095613A1 (en) | 2020-03-26 |
WO2014074895A3 (en) | 2014-07-03 |
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Legal Events
Date | Code | Title | Description |
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B06F | Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette] | ||
B06F | Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette] | ||
B06I | Publication of requirement cancelled [chapter 6.9 patent gazette] |
Free format text: ANULADA A PUBLICACAO CODIGO 6.6.1 NA RPI NO 2462 DE 13/03/2018 POR TER SIDO INDEVIDA. |
|
B25G | Requested change of headquarter approved |
Owner name: LALLEMAND HUNGARY LIQUIDITY MANAGEMENT LLC (HU) |
|
B06U | Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette] | ||
B11B | Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements |