BR112014025437A2 - aumento de estabilidade de proteína em plantas transgênicas - Google Patents

aumento de estabilidade de proteína em plantas transgênicas

Info

Publication number
BR112014025437A2
BR112014025437A2 BR112014025437A BR112014025437A BR112014025437A2 BR 112014025437 A2 BR112014025437 A2 BR 112014025437A2 BR 112014025437 A BR112014025437 A BR 112014025437A BR 112014025437 A BR112014025437 A BR 112014025437A BR 112014025437 A2 BR112014025437 A2 BR 112014025437A2
Authority
BR
Brazil
Prior art keywords
transgenic plants
protein
fusion proteins
interest
protein stability
Prior art date
Application number
BR112014025437A
Other languages
English (en)
Inventor
Cheol Jang In
Hai Chua Nam
Niu Qiwen
Deng Shulin
Original Assignee
Univ Rockefeller
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Rockefeller filed Critical Univ Rockefeller
Publication of BR112014025437A2 publication Critical patent/BR112014025437A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Abstract

resumo patente de invenção: "aumento de estabilidade de proteína em plantas transgênicas". a presente invenção refere-se a composições e métodos para aumentar a estabilidade de proteínas em plantas transgênicas. as composições são construtos de ácido nucleico que codificam proteínas de fusão, proteínas de fusão, células de plantas transgênicas e plantas transgênicas. uma proteína de fusão de acordo com a presente invenção compreende uma proteína de interesse e um domínio uba1 ou uba2 de uma proteína rad23 de arabidopsis. os métodos usam os construtos de ácido nucleico para produzir proteínas de fusão em células de plantas transgênicas ou plantas transgênicas. as proteínas de fusão têm uma estabilidade maior do que a proteína de interesse e têm a mesma função que as proteínas de interesse.
BR112014025437A 2012-04-13 2013-03-13 aumento de estabilidade de proteína em plantas transgênicas BR112014025437A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261623794P 2012-04-13 2012-04-13
PCT/US2013/030953 WO2013154730A1 (en) 2012-04-13 2013-03-13 Enhancing protein stability in transgenic plants

Publications (1)

Publication Number Publication Date
BR112014025437A2 true BR112014025437A2 (pt) 2017-08-08

Family

ID=49328019

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112014025437A BR112014025437A2 (pt) 2012-04-13 2013-03-13 aumento de estabilidade de proteína em plantas transgênicas

Country Status (7)

Country Link
US (1) US20150074847A1 (pt)
CN (1) CN104487578A (pt)
AU (1) AU2013246414A1 (pt)
BR (1) BR112014025437A2 (pt)
CA (1) CA2870251A1 (pt)
RU (1) RU2014145425A (pt)
WO (1) WO2013154730A1 (pt)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109666693B (zh) * 2018-12-29 2022-08-16 北京市农林科学院 Mg132在碱基编辑***编辑受体基因组中的应用
CN110606894B (zh) * 2019-07-31 2022-12-09 电子科技大学 提高基因编辑效率的Cas9融合蛋白、编码基因及其应用
EP4365294A1 (en) * 2021-06-16 2024-05-08 Anyang Institution Of Sino-Cotton Industry Innovation Application of plant jaz proteins and derived polypeptides in prevention and treatment of human and animal tumors

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002061100A1 (en) * 2001-01-30 2002-08-08 Wisconsin Alumni Research Foundation Expression of multiple proteins in transgenic plants
US20110250613A1 (en) * 2009-06-15 2011-10-13 The Johns Hopkins University Arrayed Multiple Ubiquitin Binding Domains as Linkage-specific Polyubiquitin Affinity Reagents

Also Published As

Publication number Publication date
RU2014145425A (ru) 2016-06-10
CN104487578A (zh) 2015-04-01
AU2013246414A1 (en) 2014-11-27
WO2013154730A1 (en) 2013-10-17
CA2870251A1 (en) 2013-10-17
US20150074847A1 (en) 2015-03-12

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Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B11B Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements