BR112014011937A2 - quantitative highly multiplexed nucleic acid detection - Google Patents

quantitative highly multiplexed nucleic acid detection

Info

Publication number
BR112014011937A2
BR112014011937A2 BR112014011937A BR112014011937A BR112014011937A2 BR 112014011937 A2 BR112014011937 A2 BR 112014011937A2 BR 112014011937 A BR112014011937 A BR 112014011937A BR 112014011937 A BR112014011937 A BR 112014011937A BR 112014011937 A2 BR112014011937 A2 BR 112014011937A2
Authority
BR
Brazil
Prior art keywords
nucleic acid
highly multiplexed
acid detection
methods
multiplexed nucleic
Prior art date
Application number
BR112014011937A
Other languages
Portuguese (pt)
Inventor
Taft Brad
Scaboo Kris
Martin Patrick
Original Assignee
Nvs Tech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/US2012/025699 external-priority patent/WO2012112925A2/en
Application filed by Nvs Tech Inc filed Critical Nvs Tech Inc
Publication of BR112014011937A2 publication Critical patent/BR112014011937A2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

resumo “detecção altamente multiplexada quantitativa de ácidos nucléicos” esta invenção proporciona métodos para detectar e quantificar ácidos nucléicos alvo em amostras em reações de câmara única multiplexada. os consumíveis incorporando as câmaras otimizados para reduzir o sinal anterior proximal para conjuntos eficazes são proporcionados, assim como os métodos de uso. os dispositivos e os sistemas configurados para usar os consumíveis para praticar os métodos são uma característica da invenção.abstract highly multiplexed quantitative detection of nucleic acids this invention provides methods to detect and quantify target nucleic acids in samples in multiplexed single chamber reactions. Consumables incorporating chambers optimized to reduce proximal anterior signal for effective assemblies are provided, as are methods of use. devices and systems configured to use the consumables to practice the methods are a feature of the invention.

BR112014011937A 2011-11-17 2012-08-16 quantitative highly multiplexed nucleic acid detection BR112014011937A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201161561198P 2011-11-17 2011-11-17
PCT/US2012/025699 WO2012112925A2 (en) 2011-02-18 2012-02-17 Quantitative, highly multiplexed detection of nucleic acids
US13/399,872 US20120214686A1 (en) 2011-02-18 2012-02-17 Quantitative, Highly Multiplexed Detection of Nucleic Acids
PCT/US2012/051236 WO2013074163A1 (en) 2011-11-17 2012-08-16 Quantitative, highly multiplexed detection of nucleic acids

Publications (1)

Publication Number Publication Date
BR112014011937A2 true BR112014011937A2 (en) 2017-05-30

Family

ID=48430026

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112014011937A BR112014011937A2 (en) 2011-11-17 2012-08-16 quantitative highly multiplexed nucleic acid detection

Country Status (12)

Country Link
EP (1) EP2780479A4 (en)
JP (1) JP2014533502A (en)
KR (1) KR20140094007A (en)
CN (1) CN104093857A (en)
AU (1) AU2012337389A1 (en)
BR (1) BR112014011937A2 (en)
CA (1) CA2855953A1 (en)
HK (1) HK1199746A1 (en)
IL (1) IL232586A0 (en)
MX (1) MX338076B (en)
SG (1) SG11201402341QA (en)
WO (1) WO2013074163A1 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20160244816A1 (en) * 2013-07-15 2016-08-25 Seegene, Inc. Detection of target nucleic acid sequence by pto cleavage and extension-dependent immobilized oligonucleotide hybridization
WO2016168640A1 (en) * 2015-04-16 2016-10-20 William Marsh Rice University Stoichiometric tuning of nucleic acid hybridization probes by auxiliary oligonucleotide species
CN110016500A (en) * 2018-01-10 2019-07-16 深圳闪量科技有限公司 Surface-probe quantifying PCR method
EP4062373A1 (en) * 2019-11-21 2022-09-28 10X Genomics, Inc. Spatial analysis of analytes
CN111808989A (en) * 2020-06-18 2020-10-23 重庆浦洛通基因医学研究院有限公司 Coronavirus/influenza virus/rhinovirus nucleic acid combined detection kit and use method thereof
KR20230085149A (en) 2020-10-15 2023-06-13 제넨테크, 인크. Multiplexed target-binding candidate screening analysis

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5422253A (en) * 1992-12-07 1995-06-06 Wisconsin Alumni Research Foundation Method of site specific nucleic acid cleavage
US6350580B1 (en) * 2000-10-11 2002-02-26 Stratagene Methods for detection of a target nucleic acid using a probe comprising secondary structure
WO2003042353A2 (en) * 2001-07-17 2003-05-22 Stratagene Methods for detection of a target nucleic acid by capture using multi-subunit probes
WO2005010199A2 (en) * 2003-07-16 2005-02-03 Geneohm Sciences, Inc. Invasive cleavage reaction with electrochemical readout
WO2005068660A1 (en) * 2003-12-19 2005-07-28 Beckman Coulter, Inc. Solid-phase multiplexed invader assay
AU2007293187A1 (en) * 2006-06-30 2008-03-13 Rosetta Genomics Ltd A method for detecting nucleic acids
WO2008083261A1 (en) * 2006-12-29 2008-07-10 Applera Corporation Systems and methods for detecting nucleic acids
EP2077336A1 (en) * 2007-12-19 2009-07-08 Koninklijke Philips Electronics N.V. Device and method for parallel quantitative analysis of multiple nucleic acids
JP2012509078A (en) * 2008-11-21 2012-04-19 コーニンクレッカ フィリップス エレクトロニクス エヌ ヴィ Real-time multiplex PCR detection on solid surface using double-stranded nucleic acid specific dye

Also Published As

Publication number Publication date
WO2013074163A1 (en) 2013-05-23
CA2855953A1 (en) 2013-05-23
SG11201402341QA (en) 2014-06-27
AU2012337389A1 (en) 2014-06-19
EP2780479A4 (en) 2015-04-08
MX2014005912A (en) 2014-09-04
MX338076B (en) 2016-04-01
JP2014533502A (en) 2014-12-15
HK1199746A1 (en) 2015-07-17
KR20140094007A (en) 2014-07-29
CN104093857A (en) 2014-10-08
IL232586A0 (en) 2014-06-30
EP2780479A1 (en) 2014-09-24

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Legal Events

Date Code Title Description
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]
B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]