BR0313045A - Processo para melhorar o rendimento da produção de proteìna recombinante de plantas - Google Patents
Processo para melhorar o rendimento da produção de proteìna recombinante de plantasInfo
- Publication number
- BR0313045A BR0313045A BR0313045-2A BR0313045A BR0313045A BR 0313045 A BR0313045 A BR 0313045A BR 0313045 A BR0313045 A BR 0313045A BR 0313045 A BR0313045 A BR 0313045A
- Authority
- BR
- Brazil
- Prior art keywords
- recombinant
- plant protein
- protein production
- recombinant plant
- plant
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/63—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from plants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Pharmacology & Pharmacy (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Botany (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
"PROCESSO PARA MELHORAR O RENDIMENTO DA PRODUçãO DE PROTEìNA RECOMBINANTE DE PLANTAS". A presente invenção refere-se a um processo para melhorar o rendimento de proteína recombinante produzida em plantas transformadas geneticamente. A invenção refere-se, mais especificamente, a um processo para prevenção de proteólise indesejável de proteínas recombinantes, após colheita da planta, durante processamento dos produtos das plantas. Especificamente, essa invenção focaliza a introdução dos inibidores de protease nas plantas, para prevenir proteólise indesejada de proteínas recombinantes quando do rompimento da célula durante o processo de extração.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US39877902P | 2002-07-29 | 2002-07-29 | |
PCT/CA2003/001141 WO2004011656A1 (en) | 2002-07-29 | 2003-07-28 | Method for enhancing yield of recombinant protein production from plants |
Publications (1)
Publication Number | Publication Date |
---|---|
BR0313045A true BR0313045A (pt) | 2005-07-05 |
Family
ID=31188478
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR0313045-2A BR0313045A (pt) | 2002-07-29 | 2003-07-28 | Processo para melhorar o rendimento da produção de proteìna recombinante de plantas |
Country Status (10)
Country | Link |
---|---|
US (1) | US20050251885A1 (pt) |
EP (1) | EP1525318A1 (pt) |
JP (1) | JP2005534300A (pt) |
CN (1) | CN1671848A (pt) |
AU (1) | AU2003257290A1 (pt) |
BR (1) | BR0313045A (pt) |
CA (1) | CA2492500A1 (pt) |
IL (1) | IL166387A0 (pt) |
MX (1) | MXPA05001034A (pt) |
WO (1) | WO2004011656A1 (pt) |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1525319A1 (en) * | 2002-07-29 | 2005-04-27 | Universit Laval | Method for enhancing the nutritive value of plant extract |
CA2453723A1 (en) * | 2002-12-20 | 2004-06-20 | Universite Laval | Method for increasing protein content in plant cells |
ATE486133T1 (de) * | 2005-07-01 | 2010-11-15 | Univ Kentucky Res Found | Transformierte pflanzen mit anreicherung von mono-und/oder sesquiterpenen |
US9597379B1 (en) | 2010-02-09 | 2017-03-21 | David Gordon Bermudes | Protease inhibitor combination with therapeutic proteins including antibodies |
US8524220B1 (en) | 2010-02-09 | 2013-09-03 | David Gordon Bermudes | Protease inhibitor: protease sensitivity expression system composition and methods improving the therapeutic activity and specificity of proteins delivered by bacteria |
US8771669B1 (en) | 2010-02-09 | 2014-07-08 | David Gordon Bermudes | Immunization and/or treatment of parasites and infectious agents by live bacteria |
US9737592B1 (en) | 2014-02-14 | 2017-08-22 | David Gordon Bermudes | Topical and orally administered protease inhibitors and bacterial vectors for the treatment of disorders and methods of treatment |
US11180535B1 (en) | 2016-12-07 | 2021-11-23 | David Gordon Bermudes | Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria |
US11129906B1 (en) | 2016-12-07 | 2021-09-28 | David Gordon Bermudes | Chimeric protein toxins for expression by therapeutic bacteria |
CN111285932B (zh) * | 2018-12-10 | 2023-07-11 | 武汉禾元生物科技股份有限公司 | 一种从基因工程水稻种子中分离纯化重组人纤维连接蛋白的方法 |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5658772A (en) * | 1989-12-22 | 1997-08-19 | E. I. Du Pont De Nemours And Company | Site-specific recombination of DNA in plant cells |
US5641876A (en) * | 1990-01-05 | 1997-06-24 | Cornell Research Foundation, Inc. | Rice actin gene and promoter |
US6127144A (en) * | 1993-12-23 | 2000-10-03 | Cangene Corporation | Method for expression of proteins in bacterial host cells |
WO1998039461A1 (en) * | 1997-03-20 | 1998-09-11 | Prodigene Inc. | Methods of commercial production and extraction of protein from seed |
US5990385A (en) * | 1997-11-10 | 1999-11-23 | Her Majesty The Queen In Right Of Canada, As Represented By The Minister Of Agriculture And Agri-Food | Protein production in transgenic alfalfa plants |
KR20010040337A (ko) * | 1998-01-16 | 2001-05-15 | 맥기 데이비드 알 | 누클레오티드 서열을 숙주로 형질감염시켜 이누클레오티드 서열의 기능 및 이 누클레오티드 서열이코딩하는 단백질을 결정하는 방법 |
FR2774379B1 (fr) * | 1998-01-30 | 2002-03-29 | Groupe Limagrain Holding | Procede de production, par des cellules vegetales, d'alpha 1-antitrypsine et de ses variantes, et produits contenant l'alpha-antitrypsine ainsi obtenue |
US6303941B1 (en) * | 1999-10-25 | 2001-10-16 | Hrl Laboratories | Integrated asymmetric resonant tunneling diode pair circuit |
US6855871B2 (en) * | 2000-08-21 | 2005-02-15 | Pioneer Hi-Bred International, Inc. | Methods of increasing polypeptide accumulation in plants |
CA2453723A1 (en) * | 2002-12-20 | 2004-06-20 | Universite Laval | Method for increasing protein content in plant cells |
-
2003
- 2003-07-28 EP EP03771029A patent/EP1525318A1/en not_active Withdrawn
- 2003-07-28 CN CNA038181460A patent/CN1671848A/zh active Pending
- 2003-07-28 JP JP2004523699A patent/JP2005534300A/ja active Pending
- 2003-07-28 WO PCT/CA2003/001141 patent/WO2004011656A1/en not_active Application Discontinuation
- 2003-07-28 MX MXPA05001034A patent/MXPA05001034A/es unknown
- 2003-07-28 BR BR0313045-2A patent/BR0313045A/pt not_active IP Right Cessation
- 2003-07-28 US US10/519,843 patent/US20050251885A1/en not_active Abandoned
- 2003-07-28 CA CA002492500A patent/CA2492500A1/en not_active Abandoned
- 2003-07-28 AU AU2003257290A patent/AU2003257290A1/en not_active Abandoned
-
2005
- 2005-01-19 IL IL16638705A patent/IL166387A0/xx unknown
Also Published As
Publication number | Publication date |
---|---|
AU2003257290A1 (en) | 2004-02-16 |
CA2492500A1 (en) | 2004-02-05 |
JP2005534300A (ja) | 2005-11-17 |
EP1525318A1 (en) | 2005-04-27 |
WO2004011656A1 (en) | 2004-02-05 |
CN1671848A (zh) | 2005-09-21 |
US20050251885A1 (en) | 2005-11-10 |
IL166387A0 (en) | 2006-01-16 |
MXPA05001034A (es) | 2005-06-08 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
B08F | Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette] |
Free format text: REFERENTE A 5A, 6A E 7A ANUIDADE(S). |
|
B08K | Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette] |
Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 2064 DE 27/07/2010. |