BR0313045A - Processo para melhorar o rendimento da produção de proteìna recombinante de plantas - Google Patents

Processo para melhorar o rendimento da produção de proteìna recombinante de plantas

Info

Publication number
BR0313045A
BR0313045A BR0313045-2A BR0313045A BR0313045A BR 0313045 A BR0313045 A BR 0313045A BR 0313045 A BR0313045 A BR 0313045A BR 0313045 A BR0313045 A BR 0313045A
Authority
BR
Brazil
Prior art keywords
recombinant
plant protein
protein production
recombinant plant
plant
Prior art date
Application number
BR0313045-2A
Other languages
English (en)
Inventor
Dominique Michaud
Daniel Rivard
Raphael Anguenot
Sonia Trepanier
Louis-Philippe Vezina
France Brunelle
Original Assignee
Univ Laval
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Laval filed Critical Univ Laval
Publication of BR0313045A publication Critical patent/BR0313045A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/63—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Botany (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

"PROCESSO PARA MELHORAR O RENDIMENTO DA PRODUçãO DE PROTEìNA RECOMBINANTE DE PLANTAS". A presente invenção refere-se a um processo para melhorar o rendimento de proteína recombinante produzida em plantas transformadas geneticamente. A invenção refere-se, mais especificamente, a um processo para prevenção de proteólise indesejável de proteínas recombinantes, após colheita da planta, durante processamento dos produtos das plantas. Especificamente, essa invenção focaliza a introdução dos inibidores de protease nas plantas, para prevenir proteólise indesejada de proteínas recombinantes quando do rompimento da célula durante o processo de extração.
BR0313045-2A 2002-07-29 2003-07-28 Processo para melhorar o rendimento da produção de proteìna recombinante de plantas BR0313045A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US39877902P 2002-07-29 2002-07-29
PCT/CA2003/001141 WO2004011656A1 (en) 2002-07-29 2003-07-28 Method for enhancing yield of recombinant protein production from plants

Publications (1)

Publication Number Publication Date
BR0313045A true BR0313045A (pt) 2005-07-05

Family

ID=31188478

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0313045-2A BR0313045A (pt) 2002-07-29 2003-07-28 Processo para melhorar o rendimento da produção de proteìna recombinante de plantas

Country Status (10)

Country Link
US (1) US20050251885A1 (pt)
EP (1) EP1525318A1 (pt)
JP (1) JP2005534300A (pt)
CN (1) CN1671848A (pt)
AU (1) AU2003257290A1 (pt)
BR (1) BR0313045A (pt)
CA (1) CA2492500A1 (pt)
IL (1) IL166387A0 (pt)
MX (1) MXPA05001034A (pt)
WO (1) WO2004011656A1 (pt)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1525319A1 (en) * 2002-07-29 2005-04-27 Universit Laval Method for enhancing the nutritive value of plant extract
CA2453723A1 (en) * 2002-12-20 2004-06-20 Universite Laval Method for increasing protein content in plant cells
ATE486133T1 (de) * 2005-07-01 2010-11-15 Univ Kentucky Res Found Transformierte pflanzen mit anreicherung von mono-und/oder sesquiterpenen
US9597379B1 (en) 2010-02-09 2017-03-21 David Gordon Bermudes Protease inhibitor combination with therapeutic proteins including antibodies
US8524220B1 (en) 2010-02-09 2013-09-03 David Gordon Bermudes Protease inhibitor: protease sensitivity expression system composition and methods improving the therapeutic activity and specificity of proteins delivered by bacteria
US8771669B1 (en) 2010-02-09 2014-07-08 David Gordon Bermudes Immunization and/or treatment of parasites and infectious agents by live bacteria
US9737592B1 (en) 2014-02-14 2017-08-22 David Gordon Bermudes Topical and orally administered protease inhibitors and bacterial vectors for the treatment of disorders and methods of treatment
US11180535B1 (en) 2016-12-07 2021-11-23 David Gordon Bermudes Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
CN111285932B (zh) * 2018-12-10 2023-07-11 武汉禾元生物科技股份有限公司 一种从基因工程水稻种子中分离纯化重组人纤维连接蛋白的方法

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5658772A (en) * 1989-12-22 1997-08-19 E. I. Du Pont De Nemours And Company Site-specific recombination of DNA in plant cells
US5641876A (en) * 1990-01-05 1997-06-24 Cornell Research Foundation, Inc. Rice actin gene and promoter
US6127144A (en) * 1993-12-23 2000-10-03 Cangene Corporation Method for expression of proteins in bacterial host cells
WO1998039461A1 (en) * 1997-03-20 1998-09-11 Prodigene Inc. Methods of commercial production and extraction of protein from seed
US5990385A (en) * 1997-11-10 1999-11-23 Her Majesty The Queen In Right Of Canada, As Represented By The Minister Of Agriculture And Agri-Food Protein production in transgenic alfalfa plants
KR20010040337A (ko) * 1998-01-16 2001-05-15 맥기 데이비드 알 누클레오티드 서열을 숙주로 형질감염시켜 이누클레오티드 서열의 기능 및 이 누클레오티드 서열이코딩하는 단백질을 결정하는 방법
FR2774379B1 (fr) * 1998-01-30 2002-03-29 Groupe Limagrain Holding Procede de production, par des cellules vegetales, d'alpha 1-antitrypsine et de ses variantes, et produits contenant l'alpha-antitrypsine ainsi obtenue
US6303941B1 (en) * 1999-10-25 2001-10-16 Hrl Laboratories Integrated asymmetric resonant tunneling diode pair circuit
US6855871B2 (en) * 2000-08-21 2005-02-15 Pioneer Hi-Bred International, Inc. Methods of increasing polypeptide accumulation in plants
CA2453723A1 (en) * 2002-12-20 2004-06-20 Universite Laval Method for increasing protein content in plant cells

Also Published As

Publication number Publication date
AU2003257290A1 (en) 2004-02-16
CA2492500A1 (en) 2004-02-05
JP2005534300A (ja) 2005-11-17
EP1525318A1 (en) 2005-04-27
WO2004011656A1 (en) 2004-02-05
CN1671848A (zh) 2005-09-21
US20050251885A1 (en) 2005-11-10
IL166387A0 (en) 2006-01-16
MXPA05001034A (es) 2005-06-08

Similar Documents

Publication Publication Date Title
Arbona et al. Common and divergent physiological, hormonal and metabolic responses of Arabidopsis thaliana and Thellungiella halophila to water and salt stress
BR0313045A (pt) Processo para melhorar o rendimento da produção de proteìna recombinante de plantas
Mohamad Ansor et al. Anti-angiotensin converting enzyme (ACE) proteins from mycelia of Ganoderma lucidum (Curtis) P. Karst
CN105392795B (zh) 生产肉毒杆菌毒素的方法
DK1539966T3 (da) Fremgangsmåde til fremstilling af proteiner
TR199800268T1 (xx) Domateslerin s�nai �apta proseslenmesi.
Unajak et al. Characterisation of thermostable trypsin and determination of trypsin isozymes from intestine of Nile tilapia (Oreochromis niloticus L.)
Gao et al. Desiccation enhances phosphorylation of PSII and affects the distribution of protein complexes in the thylakoid membrane
DE60115031D1 (de) Spermatophyte pflanzenextrakte mit antitumoraler wirkung
Park et al. Proteomic analysis of host cell protein dynamics in the supernatant of Fc‐fusion protein‐producing CHO DG44 and DUKX‐B11 cell lines in batch and fed‐batch cultures
NZ511076A (en) Method to mitigate plant stress by treatment with a composition containing gamma aminobutyric acid
EA200500993A1 (ru) Извлечение ингредиентов из биологического материала
Antoine et al. Short‐and long‐term effects of carbohydrate limitation on sugar and organic acid accumulation during mandarin fruit growth
DE60309893D1 (de) Verfahren zum trennen von rosettenpflanzen
DK1268399T3 (da) Fremgangsmåde til separering af en basisk aminosyre fra fermenteringsvæske
Sun et al. Activity‐based mass spectrometric characterization of proteases and inhibitors in human saliva
NO20053677L (no) Fremgangsmate for fremstilling av albumin
WO2005021762A3 (en) A process for proteolytic cleavage and purification of recombinant proteins produced in plants
BR0314915A (pt) Planta leguminosa, vetor de transformação e método para a obtenção de plantas leguminosas
CN110129394B (zh) 一种具抗疲劳功效的牡丹肽及其制备方法
BR0313015A (pt) Processo para melhorar o valor nutritivo de um extrato vegetal
Badgujar et al. Peptide Mass Fingerprinting and N‐Terminal Amino Acid Sequencing of Glycosylated Cysteine Protease of Euphorbia nivulia Buch.‐Ham.
CN106879884B (zh) 一种提高蜂王浆性能的方法
Kondratiuk et al. Optimization of protein extraction for lichen thalli
CN102018958B (zh) 去除疫苗制品中杂蛋白及宿主dna的方法

Legal Events

Date Code Title Description
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE A 5A, 6A E 7A ANUIDADE(S).

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 2064 DE 27/07/2010.