BR0306956A - variantes de protease multiplamente substituìdas - Google Patents

variantes de protease multiplamente substituìdas

Info

Publication number
BR0306956A
BR0306956A BRPI0306956-7A BR0306956A BR0306956A BR 0306956 A BR0306956 A BR 0306956A BR 0306956 A BR0306956 A BR 0306956A BR 0306956 A BR0306956 A BR 0306956A
Authority
BR
Brazil
Prior art keywords
protease
protease variants
amino acid
precursor
variants
Prior art date
Application number
BRPI0306956-7A
Other languages
English (en)
Inventor
Ayrookaran J Poulose
Original Assignee
Genencor Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor Int filed Critical Genencor Int
Publication of BR0306956A publication Critical patent/BR0306956A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21062—Subtilisin (3.4.21.62)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

"VARIANTES DE PROTEASE MULTIPLAMENTE SUBSTITUìDAS". A presente invenção refere-se a novas variantes de protease derivadas das seqüências de DNA de proteases não-humanas que ocorrem naturalmente ou recombinantes. As variantes de proteases, em geral, são obtidas por modificação in vitro de uma seqüência precursora de DNA que codifica a protease que ocorre naturalmente ou recombinante para gerar a substituição de um grande número de resíduos de aminoácido na seqüência de aminoácido de uma protease precursora. Tais variantes de protease têm propriedades que são diferentes daquelas da protease precursora, tal como desempenho de lavagem alterado. O resíduo de aminoácido substituído equivalente às posições 7, 23, 26, 28, 29, 30, 31, 47, 66, 69, 73, 82, 85, 88, 90, 92, 93, 105, 113, 139, 148, 149, 150, 151, 178, 200, 201, 231, 233, 267 e/ou 273 da subtilisina do Bacillus amyloquefaciens.
BRPI0306956-7A 2002-01-16 2003-01-16 variantes de protease multiplamente substituìdas BR0306956A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US35022102P 2002-01-16 2002-01-16
PCT/US2003/001447 WO2003062380A2 (en) 2002-01-16 2003-01-16 Multiply-substituted protease variants

Publications (1)

Publication Number Publication Date
BR0306956A true BR0306956A (pt) 2006-04-11

Family

ID=27613371

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0306956-7A BR0306956A (pt) 2002-01-16 2003-01-16 variantes de protease multiplamente substituìdas

Country Status (8)

Country Link
US (5) US20050170488A1 (pt)
EP (3) EP2287321B1 (pt)
JP (4) JP5328002B2 (pt)
AU (1) AU2003210551A1 (pt)
BR (1) BR0306956A (pt)
CA (1) CA2472723C (pt)
MX (1) MXPA04006811A (pt)
WO (1) WO2003062380A2 (pt)

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US7985569B2 (en) 2003-11-19 2011-07-26 Danisco Us Inc. Cellulomonas 69B4 serine protease variants
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CA2680563A1 (en) * 2007-03-12 2008-09-18 Danisco Us Inc. Modified proteases
CA2690055A1 (en) 2007-06-06 2008-12-18 Danisco Us Inc. Methods for improving protein performance
EP3399031B1 (en) * 2014-12-15 2019-10-30 Henkel AG & Co. KGaA Detergent composition comprising subtilase variants
CN108291179B (zh) 2015-11-25 2020-07-07 荷兰联合利华有限公司 液体洗涤剂组合物
FI3419991T3 (fi) 2016-03-04 2023-01-31 Modifioidut ribosomaaliset promoottorit proteiinien tuottamiseksi mikro-organismeissa
DE102016204814A1 (de) 2016-03-23 2017-09-28 Henkel Ag & Co. Kgaa Verbesserte Reinigungsleistung an Protein sensitiven Anschmutzungen
WO2019089898A1 (en) 2017-11-02 2019-05-09 Danisco Us Inc Freezing point depressed solid matrix compositions for melt granulation of enzymes

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Also Published As

Publication number Publication date
JP2015198660A (ja) 2015-11-12
WO2003062380A2 (en) 2003-07-31
JP5328002B2 (ja) 2013-10-30
JP2005529584A (ja) 2005-10-06
EP2287321A1 (en) 2011-02-23
JP6792524B2 (ja) 2020-11-25
JP2017225449A (ja) 2017-12-28
US20150232829A1 (en) 2015-08-20
US20130157341A1 (en) 2013-06-20
US20050170488A1 (en) 2005-08-04
US20180155702A1 (en) 2018-06-07
EP1530631B1 (en) 2013-08-07
JP5863679B2 (ja) 2016-02-17
CA2472723A1 (en) 2003-07-31
CA2472723C (en) 2013-12-17
AU2003210551A1 (en) 2003-09-02
WO2003062380A3 (en) 2005-03-24
MXPA04006811A (es) 2004-10-11
JP2013116110A (ja) 2013-06-13
EP2287321B1 (en) 2014-08-13
EP1530631A2 (en) 2005-05-18
EP1530631A4 (en) 2006-03-08
US20120052553A1 (en) 2012-03-01
EP2287320A1 (en) 2011-02-23
EP2287320B1 (en) 2014-10-01

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Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07A Technical examination (opinion): publication of technical examination (opinion) [chapter 7.1 patent gazette]
B25D Requested change of name of applicant approved

Owner name: DANISCO US INC. (US)

B09B Patent application refused [chapter 9.2 patent gazette]

Free format text: INDEFIRO O PEDIDO DE ACORDO COM O(S) ARTIGO(S) 22, 8O, 11, 13, 25 DA LPI

B12B Appeal: appeal against refusal