BR0207239A - Polynucleotides encoding chromosome gene of the bacterium chromobacterium violaceum, expression and activity of these polynucleotides and their applications - Google Patents
Polynucleotides encoding chromosome gene of the bacterium chromobacterium violaceum, expression and activity of these polynucleotides and their applicationsInfo
- Publication number
- BR0207239A BR0207239A BR0207239A BR0207239A BR0207239A BR 0207239 A BR0207239 A BR 0207239A BR 0207239 A BR0207239 A BR 0207239A BR 0207239 A BR0207239 A BR 0207239A BR 0207239 A BR0207239 A BR 0207239A
- Authority
- BR
- Brazil
- Prior art keywords
- sym
- genes
- chromobacterium violaceum
- polynucleotides
- bacteria
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/22—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Neisseriaceae (F)
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Abstract
"POLINUCLEOTìDEOS CODIFICADORES DE GENES DO CROMOSSOMO DA BACTéRIA Chromobacterium violaceum, EXPRESSãO E ATIVIDADE DESSES POLINUCLEOTìDEOS E SUAS APLICAçõES". Chromobacterium violaceum desenvolveu a capacidade de adaptação a alterações ambientais bruscas, incluindo a disponibilidade de nutrientes, mudanças na temperatura, presença de elementos tóxicos, entre outras. Diversos genes desse organismos foram identificados e todos os mecanismos de transcrição, como as polimerases do RNA e os fatores sigma foram anotados, tais como <sym>^ 70^ (rpoD), <sym>^ 54^ (rpoN), <sym>^ 32^ (rpoH), <sym>^ 38^ (rpoS), <sym>^ 28^ (fliA), <sym>^ 24^ (rpoE), anti-<sym>^ 28^ fator (flgM), relacionado a <sym>^ 54^ (yhbH) e Ecf-<sym>^ 70^. Além disso, diversos ativadores transcricionais e repressores que interagem com os fatores sigma e envolvidos na resposta das bactérias a estresses ambientais também estão presentes, com diversos genes relacionados às famílias LysR, AraC, TetR, GntR, Mar, Mer, AsnC, DeoR, AsrR, Lacl, Crp, entre outros. Ainda mais expressiva foi a detecção de um número elevado de proteínas regulatórias associadas à adaptação a diversos ambientes, representando quase 9% dos genes de Chromobacterium violaceum. A versatilidade de Chromobacterium violaceum também fica evidenciada nos mais de 300 genes identificados e relacionados ao metabolismo energético, o que permite a obtenção de energia em condições aeróbicas, microaeróbidas e anaeróbicas. Foram detectados, também, genes relacionados à absorção de determinados nutrientes em mecanismos conhecidos por conferir, às bactérias, vantagens na competição contra outros microrganimos e, ainda, diversos genes capazes de promover a sobrevivência em condições de limitação de nutrientes, como genes envolvidos na utilização de peptídeos sob estresse de carbono, as proteínas SspA e SspB, induzidas por deficiência de glicose, nitrogênio, fosfato e aminoácidos , as proteínas de estresse "universal stress proteins" (Usp), que normalmente agem sob estresses de temperaturas elevadas, salinidade e exposição aos raios ultra-violeta, entre outras. Determinadas funcionalidades genómicas foram estabelecidas."BACTERIA CHROMOSOME GENE ENCODER POLYNUCLEOTIDES Chromobacterium violaceum, EXPRESSION AND ACTIVITY OF THESE POLINUCLEOTIDES AND THEIR APPLICATIONS". Chromobacterium violaceum has developed the ability to adapt to sudden environmental changes, including nutrient availability, temperature changes, presence of toxic elements, among others. Several genes of these organisms were identified and all transcriptional mechanisms such as RNA polymerases and sigma factors were noted, such as <sym> ^ 70 ^ (rpoD), <sym> ^ 54 ^ (rpoN), <sym> ^ 32 ^ (rpoH), <sym> ^ 38 ^ (rpoS), <sym> ^ 28 ^ (fliA), <sym> ^ 24 ^ (rpoE), anti-<sym> ^ 28 ^ factor (flgM), related to <sym> ^ 54 ^ (yhbH) and Ecf- <sym> ^ 70 ^. In addition, several transcriptional activators and repressors that interact with sigma factors involved in the response of bacteria to environmental stresses are also present, with several genes related to the LysR, AraC, TetR, GntR, Mar, Mer, AsnC, DeoR, AsrR families. , Lacl, Crp, among others. Even more significant was the detection of a large number of regulatory proteins associated with adaptation to various environments, representing almost 9% of the Chromobacterium violaceum genes. The versatility of Chromobacterium violaceum is also evident in the more than 300 genes identified and related to energy metabolism, allowing energy to be obtained under aerobic, microaerobic and anaerobic conditions. Genes related to the absorption of certain nutrients were also detected in mechanisms known to give bacteria advantages in competition against other microorganisms, as well as several genes capable of promoting survival in conditions of nutrient limitation, such as genes involved in the utilization of certain nutrients. of carbon-stressed peptides, SspA and SspB proteins, induced by glucose, nitrogen, phosphate and amino acid deficiency, the universal stress proteins (Usp), which normally act under high temperature, salinity and exposure stresses. ultraviolet rays, among others. Certain genomic functionalities have been established.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| BR0207239A BR0207239A (en) | 2002-12-19 | 2002-12-19 | Polynucleotides encoding chromosome gene of the bacterium chromobacterium violaceum, expression and activity of these polynucleotides and their applications |
| PCT/BR2003/000207 WO2004056960A2 (en) | 2002-12-19 | 2003-12-16 | Gene-coding polynucleotides of the chromosome of the bacterium chromobacterium violaceum, expression and activity of these polynucleotides and their applications |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| BR0207239A BR0207239A (en) | 2002-12-19 | 2002-12-19 | Polynucleotides encoding chromosome gene of the bacterium chromobacterium violaceum, expression and activity of these polynucleotides and their applications |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR0207239A true BR0207239A (en) | 2004-08-10 |
Family
ID=32660710
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR0207239A Pending BR0207239A (en) | 2002-12-19 | 2002-12-19 | Polynucleotides encoding chromosome gene of the bacterium chromobacterium violaceum, expression and activity of these polynucleotides and their applications |
Country Status (2)
| Country | Link |
|---|---|
| BR (1) | BR0207239A (en) |
| WO (1) | WO2004056960A2 (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8951585B2 (en) | 2010-02-25 | 2015-02-10 | Marrone Bio Innovations, Inc. | Compositions and methods for modulating plant parasitic nematodes |
| WO2011110932A1 (en) | 2010-03-12 | 2011-09-15 | Council Of Scientific & Industrial Research | Process for the production of violacein and its derivative deoxyviolacein containing bioactive pigment from chromobacterium sp. (mtcc 5522) |
| US9392790B2 (en) | 2011-05-06 | 2016-07-19 | The Research Foundation For The State University Of New York | Molecular roadblocks for RpoN binding sites |
| US8808719B1 (en) | 2013-03-15 | 2014-08-19 | Marrone Bio Innovations, Inc. | Use of Chromobacterium substugae formulations, compostions and compounds to modulate cornworm rootworm larvae infestation |
| US10160976B2 (en) | 2014-09-11 | 2018-12-25 | Marrone Bio Innovations, Inc. | Chromobacterium subtsugae genome |
| CN116694590A (en) * | 2022-04-06 | 2023-09-05 | 深圳蓝晶生物科技有限公司 | Engineered microorganisms expressing acetoacetyl-CoA reductase variants and methods for increasing the proportion of 3-hydroxycaproic acid in PHA |
-
2002
- 2002-12-19 BR BR0207239A patent/BR0207239A/en active Pending
-
2003
- 2003-12-16 WO PCT/BR2003/000207 patent/WO2004056960A2/en not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| WO2004056960A3 (en) | 2005-02-10 |
| WO2004056960A2 (en) | 2004-07-08 |
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Legal Events
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| B06F | Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette] | ||
| B08E | Application fees: payment of additional fee required [chapter 8.5 patent gazette] |
Free format text: REFERENTE A 7A ANUIDADE, GUIA 220901496771 DE 23/03/2009. |
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| B15G | Petition not considered as such [chapter 15.7 patent gazette] |
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| B08F | Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette] |
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| B08G | Application fees: restoration [chapter 8.7 patent gazette] | ||
| B07A | Application suspended after technical examination (opinion) [chapter 7.1 patent gazette] | ||
| B09B | Patent application refused [chapter 9.2 patent gazette] |
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