BR0013896A - Nucleotide sequence, vector, transformed filamentous fungi, method of producing a protein of interest, protein, and, expression system to produce a protein of interest - Google Patents
Nucleotide sequence, vector, transformed filamentous fungi, method of producing a protein of interest, protein, and, expression system to produce a protein of interestInfo
- Publication number
- BR0013896A BR0013896A BR0013896-7A BR0013896A BR0013896A BR 0013896 A BR0013896 A BR 0013896A BR 0013896 A BR0013896 A BR 0013896A BR 0013896 A BR0013896 A BR 0013896A
- Authority
- BR
- Brazil
- Prior art keywords
- protein
- interest
- producing
- vector
- produce
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0055—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10)
- C12N9/0057—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10) with oxygen as acceptor (1.10.3)
- C12N9/0061—Laccase (1.10.3.2)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2445—Beta-glucosidase (3.2.1.21)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2477—Hemicellulases not provided in a preceding group
- C12N9/248—Xylanases
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01021—Beta-glucosidase (3.2.1.21)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
"SEQuêNCIA DE NUCLEOTìDEO, VETOR, FUNGOS FILAMENTOSOS TRANSFORMADOS, MéTODO DE PRODUZIR UMA PROTEìNA DE INTERESSE, PROTEìNA, E, SISTEMA DE EXPRESSãO PARA PRODUZIR UMA PROTEìNA DE INTERESSE". A presente invenção refere-se ao aumento da produção de uma proteina de interesse de um hospedeiro fúngico. A invenção descreve seq³ências de nucleotídeo compreendendo uma região reguladora em associação operativa com uma seq³ência de secreção de xilanase e um gene de interesse. O gene de interesse codifica uma proteina selecionada de farmacêutico, nutracêutico, industrial, alimentação animal, aditivo alimenticio ou uma enzima. Preferivelmente, o gene de interesse codifica uma celulase, hemicelulase, uma enzima de degradação da lignina, pectinase, protease ou peroxidase. A presente invenção também refere-se a vetores e hospedeiros compreendendo estas seq³ências de ácido nucleico e a métodos para a produção de uma proteina de interesse."NUCLEOTIDE SEQUENCE, VECTOR, TRANSFORMED FILMARY FUNGI, METHOD OF PRODUCING A PROTEIN OF INTEREST, PROTEIN, AND, EXPRESSION SYSTEM TO PRODUCE A PROTEIN OF INTEREST". The present invention relates to increasing the production of a protein of interest to a fungal host. The invention describes nucleotide sequences comprising a regulatory region in operative association with a xylanase secretion sequence and a gene of interest. The gene of interest encodes a protein selected from pharmaceutical, nutraceutical, industrial, animal feed, food additive or an enzyme. Preferably, the gene of interest encodes a cellulase, hemicellulase, a lignin-degrading enzyme, pectinase, protease or peroxidase. The present invention also relates to vectors and hosts comprising these nucleic acid sequences and methods for producing a protein of interest.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US09/392,476 US6939704B1 (en) | 1998-03-10 | 1999-09-09 | Enhanced expression of proteins in genetically modified fungi |
PCT/CA2000/000997 WO2001018218A1 (en) | 1999-09-09 | 2000-09-01 | Expression of proteins in genetically modified fungi |
Publications (1)
Publication Number | Publication Date |
---|---|
BR0013896A true BR0013896A (en) | 2002-05-14 |
Family
ID=23550748
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR0013896-7A BR0013896A (en) | 1999-09-09 | 2000-09-01 | Nucleotide sequence, vector, transformed filamentous fungi, method of producing a protein of interest, protein, and, expression system to produce a protein of interest |
Country Status (7)
Country | Link |
---|---|
EP (1) | EP1214431A1 (en) |
CN (1) | CN1390260A (en) |
AU (1) | AU6974400A (en) |
BR (1) | BR0013896A (en) |
CA (1) | CA2382609C (en) |
MX (1) | MX250271B (en) |
WO (1) | WO2001018218A1 (en) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101967490B (en) | 2002-06-14 | 2014-07-16 | 先正达公司 | Xylanases, nucleic adics encoding them and methods for making and using them |
DK1989302T3 (en) | 2006-02-14 | 2018-07-23 | Bp Corp North America Inc | XYLANASES, NUCLEIC ACID CODING THEM AND PROCEDURES FOR THEIR PREPARATION AND USE |
US8198046B2 (en) | 2006-07-11 | 2012-06-12 | Danisco Us Inc. | KEX2 cleavage regions of recombinant fusion proteins |
US20080026376A1 (en) * | 2006-07-11 | 2008-01-31 | Huaming Wang | KEX2 cleavage regions of recombinant fusion proteins |
US8518684B2 (en) | 2008-11-18 | 2013-08-27 | Novozymes, Inc. | Methods and compositions for degrading cellulosic material |
CN111690629B (en) * | 2020-05-29 | 2022-04-19 | 浙江工业大学 | Endoglucanase mutant, gene, engineering bacterium and application thereof |
CN114426987B (en) * | 2022-01-14 | 2024-05-24 | 湖南省蔬菜研究所 | Genetic transformation method for capsicum southern blight germ |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0599859A1 (en) * | 1991-06-25 | 1994-06-08 | Novo Nordisk A/S | Mammalian pancreatic lipase and variant thereof |
US5591619A (en) * | 1994-09-30 | 1997-01-07 | University Of Georgia Research Foundation, Inc. | Aureobasidium pullulans xylanase, gene and signal sequence |
US6015703A (en) * | 1998-03-10 | 2000-01-18 | Iogen Corporation | Genetic constructs and genetically modified microbes for enhanced production of beta-glucosidase |
-
2000
- 2000-09-01 BR BR0013896-7A patent/BR0013896A/en not_active Application Discontinuation
- 2000-09-01 CN CN 00815509 patent/CN1390260A/en active Pending
- 2000-09-01 CA CA2382609A patent/CA2382609C/en not_active Expired - Fee Related
- 2000-09-01 MX MXPA02002516 patent/MX250271B/en active IP Right Grant
- 2000-09-01 EP EP00958054A patent/EP1214431A1/en not_active Withdrawn
- 2000-09-01 WO PCT/CA2000/000997 patent/WO2001018218A1/en not_active Application Discontinuation
- 2000-09-01 AU AU69744/00A patent/AU6974400A/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
CA2382609A1 (en) | 2001-03-15 |
MXPA02002516A (en) | 2005-11-17 |
MX250271B (en) | 2007-10-12 |
CA2382609C (en) | 2012-08-07 |
WO2001018218B1 (en) | 2001-04-19 |
AU6974400A (en) | 2001-04-10 |
WO2001018218A9 (en) | 2002-11-07 |
EP1214431A1 (en) | 2002-06-19 |
CN1390260A (en) | 2003-01-08 |
WO2001018218A1 (en) | 2001-03-15 |
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Legal Events
Date | Code | Title | Description |
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B11A | Dismissal acc. art.33 of ipl - examination not requested within 36 months of filing | ||
B11Y | Definitive dismissal - extension of time limit for request of examination expired [chapter 11.1.1 patent gazette] |