AU9725898A - Drug targeting system, method for preparing same and its use - Google Patents

Description

1

AUSTRALIA

Patents Act 1990 COMPLETE

SPECIFICATION

FOR A STANDARD

PATENT

I

S* Name of Applicant: Actual Inve ntor: Address for Service: Inventio Title: MEDINOVA MEDICAL

GMBH

CONSULTING

Jorg Kreuter Dixnitri A. Karkevich Bernhard Sabel Renad N. Alyautdin CULLEN CO., Patent-& Trade Mark Attorneys, 240 Queen Street, Brisbane, Qid. 4000, Aus tralia.

DRUG TARGETING SYSTEM, METHOD FOR PREPARI NG SAME AND ITS USE The following'statement is-a filldescription of-this invention, including the best method of performtn it knowl~n to u 'Cn us ~p.

i-L- Field of the Invention This invention relates to both a novel and useful method of targeting and delivering drugs and diagnostics to the brain and a drug targeting system itself. More Sparticularly, the invention pertains to a nanosphere drug ta:geting system which allows any drug ("drug as used herein includes any substance given for therapeutic and/or diagnostic purposes) to cross the blood-brain barrier (bbb) to achieve one or more of the following benefits: reducing the dose of a drug or diagnostic given peripherally, allowing drugs that .1 normally do not cross the bbb to penetrate into the brain, and reducing the peripheral side S effects by improving the relative amount of the drug reaching the brain.

Background of the Invention General Pharmacology Principles of BBB The treatment of nervous system disorders can be achieved by giving drugs which affect nervous system function or dysfunction in animals or patients. Typically, such S drugs are given by peripheral application, either via the oral or the systemic route. While many drugs are able to cross the bbb, others do not pass the bbb efficiently or not at all and are only effective when given directly into the brain. The term "blood-brain barrier" or "bbb", as used herein, refers to the bbb proper as well as to the blood-spinal barrier. The blood-brain barrier, which consists of the endothelium of the brain vessels, the basal O membrane and neuroglial cells, acts to limit penetration of substances into the brain.

Sometimes the structure of the bbb is subdivided into two components: the endothelial or Scapillary barrier and the ependymal barrier Banks, Ktin, Barrera, "Delivering peptides'to the central nervous system: Dilemmas and strategies," Pharm. Res. :1345- 1350(1991). The nature of the substance penetration through the bbb has not yet been determined but it is known that many of the reglators of brain function such as cytokines, S transferrin, encephalins and endorphines can pass through the blood vessels into Sthe brainRaeissi, Audus, "In vitro characterization of blood-brain barrier permeability to delta sleepinducing pep-tide:J. Pharnm..Phy 1:848-852(1989); Zlokovich, Susie SV.T., Davson, H. Begley,DJ., Jankov, R.M.,Mitrivic, Lipovac, "Saturable I- mechanism for delta sleep-inducing peptide (DSIP) at the blood-brain barrier of the vasculary refused guinea pig brain." n gid.10:249-254(1989);.and Zlokovich, B. "In vivo S- -_pproaches for studying peptide interactioni at the blood-brainbarrier." Conto Rel -2- 11:185-201(1990). However, many substances which can affect the Central Nervous System (or CNS) such as adenosine,p-endarphin, syithetic analogs of endogenous peptides Houghten, R.A. Swann, R. Li, "P-Endorphin: Stability, clearance behaviour and entry into the central nervous system after intravenous injection of the tritiatd peptide in rats i and rabbits." Proc. Nal. Acad. Sci. USA 77:4588-4591(1980); Levin, Frank, H.J.K., i Weber, Ismail. Mills "Studies on penetration of the blood-brain barrier by atrial natriuretic factor." Bioch n. Biophvs. Res. Commun. 14:1226-1231(1987) Sakane, ST., Tanaka, Yamamoto, Hashida, Sesaki, Ueda, Takagi, "The effect of polysorbate 80 onbrain uptake and analgetic effect of D-kyoto." Int. Pharm. :77- 83(1989), as well as some excitatory and inhibitor amino acids and trophic factors, penetrate Spoorly or not at all through the bbb. At present, drugs with no bbb penetration or poor bbb penetration can only be given by direct CNS infusion or by implantation of controlled-release S| polymers. (See, U.S. Patent No. 4,883,666, Sabel et al.) Thus, many potentially potent drugs are not useful clinically due to their inability topass the bbb.

i In addition, many drugs exist today which affect the brain in a desirable I manner but cannot be used because they have severe side effects because they affect peripheral organs of the body and/or the peripheral nervous system. Because ofthis there is a long-felt need to reduce the side effects of drugs directed to the CNS while reducing the drugs' activity in peripheral organs and increasing the action in the nervous system.

Overcoming the BBB by Difference Approaches S One way to overcome these limitations of traditional drug therapy is to S increase the relative amount of drug which passes the bbb. The reasoning is that if one can Sincrease the amount of the drug crossing the bbb while reducing the peripheral dose of a givendrug or diagnostic substance, the peripheral side effects ofthe drug are also less severe, while at the same time maintning th desired effect in the brain.

SA number of approaches have been described in the prior art to increase drug penetration through the bbb.

One approach has been to alter the function of the bbb itself. For instance, osmotic agents, when given peripherally (such as by intravenous injection), result in the opening of the bbb. Further, some drugs acting on the CNS can change the permeability of I the bbb for other substances; cholinommimetic aecolines, for instance, have been reported Sto induce changes of drug penetration through the bbb Saija, Princi, De Pasquale, R., Costa, G"Arecoline but not haloperidol produces changesin the permeability ofthe bloodb it barrier in therat-" J rm Pha. 42 135-138(1990) Other drugs which can be given to alter the permeability of the bbb are disclosed in U.S. Patent Nos. 5,059,415 and 5,124,146, both issued to E.A. Neuwelt Bradykinin is one specific drug with such effects. Patent No. 5,112,596, issued to Malfroy-Camine). Another method comprises giving permeabilizer peptides such as A-7 or conformational analogs thereof. (WO 92/18529, an application of J.W. Kozarich etal.). A relatively invasive method has been proposed by A. Tomasz and E. Tuomanen (WO 91/16064) who give parenteral injections of purified cell wall or cell wall fragments of eubacteria such as Streptococcuspneumoniae to open the bbb.

U.S. Patent No. 5,260,210 issued to L.L. Rubin et al., discloses a method whereby the permeability of the blood-brain barrier is increased by giving an agent which reduces or interferes with cyclic AMP concentrations or which increases cyclic GMP concentrations.

Any method of changing the permeability of the bbb itself, however, is t s compromised by the fact that unwanted molecules which the brain is normally protected from by the bbb can pass the bbb as well and exert undesirable side effect. Further, such an effect is non-specific so these methods are impractical due to unpredictable and uncontrollable .consequences to the nervous tissue.

Another approach is the modification of the drug molecules themselves.

For instance, macromolecules, such as proteins, do not pass the bbb at all. For example, one S" can first isolate the macromolecule active site, the portion of the molecule which triggers the biologically desirable event, and then use only this active site. Since size is one of the factors in allowing permeability of the bbb, the reduced size is used in the hope that the S smaller molecule can now pass the bbb. Other modifications to macromolecules to attempt passage :of the bbb include glycating the proteins, thereby enhanicing their permeability of the l bbb, or forming a prodrug. U.S. Patent No. 5,260,308, issued to J:F. Podusio and G.L.

Curran, discusses glycating proteins, while U.S. Patent No. 4,933,324 and WO 89/07938, Sboth on applications of V.E. Shashoua, disclose formation of a prodrug. These prodrugs are formed from a fatty acid carrier and a neuroactive drug which is unable to pass across the bbb Son its own. A similar system is disclosed in WO 89/07938.

Still another approach is the implantation of controlled release polymers Swhich release the active ingredient from a matrix-system directly into the nervous tissue.

.However, this approach is invasive and requires surgical intervention if implanted directly into the brain or spinal cord (see Sabel et al. US. PatentNo. 4,883,666; and Sabel et a. U.S.

SPatent Application Serial No. 07/407,930.) i-.

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-4- To overcome these limitations, another approach has been tried in which drug carrier systems are used such as liposomes, erythrocyte ghosts, antibody-conjugates-and- r- monoclonal antibody conjugates. One of the major problems in targeted drug delivery is the H rapid opsonization and uptake of injected carriers by the reticuloendothelial system (RES), especially by the macrophages in the liver and spleen. This obstacle may be partially overcome in the case of liposomes by incorporation of so-called "stealth" lipids, such as phosphatidylinosital, monosialoganglioside, or sulfogalactosylceramide. However, all of these systems lack the versatility to permit a wide-range application in medicine. These systems are all rather specific for particular purposes or particular drugs or diagnostic agents as the discussion of the prior art disclosures now documents: U.S. Patent Nos. 5,182,107 and 5,154,924, both issued to P.M. Friden, teach a method conjugating a drug with an antibody wherein said antibody isreactive with a transferrin receptor. Transferrin receptors are located on brain capillary endothelial cells, which thus transport a drug, such as nerve growth factor, across the bbb. U.S. Patent No.

5,004,697 (issued to Pardridge) improves such an antibody-conjugate method by providing Scationized antibodies with a specific isoelectric point (see also WO 89/01343 by Pardridge).

Another approach is to create chimeric peptides to which the active agents are S conjugated Patent No. 4,801,575, also issued to Pardridge). Such a system is further S discussed also in U.S. Patent No. 4,902,505, issued to Pardridge and Schimmel, in which the chimeric peptide, such as histone, is capable of crossing the bbb by transcytosis.

U.S. Patent Nos. 5,187,158 and 5,017,566, both issued to N.S. Bodor, disclose a brain-specific drug delivery method wherein a centrally acting drug is given with the ieduced, biooxidizable lipoidal form of a dihydropyridine recreaction-pyridine salt redox t" carrier such as dopamine. (See also U.S. Patent No. 4,880,816, also issued to Bodor).

A rather invasive approach is taken to deliver genetic material to the brain.

SThis is done by chemically disrupting the bbb and then using viruses to deliver genes across the bbb. (Se. U.S. Patent No. 4,866,042, issued to E.A. Neuwelt). Here, a corrective genetic material is incorporated into a virus and the virus is then injected into the bloodstream.

Finally, yet another carrier system to deliver drugs across the bbb is the use of liposomes, as disclosed by F.D. Collins and R.C. Thompson(WO 91/04014). Here, liposomes are targeted to specific endogenous brain transport systems which transport specific igands across the bbb. However, this system does not allow "non-penetrating" drugs to pass the bbb at all and is therefore very different from the present inventicon.

In summary, while only the carrier system described above leaves the molecule of the bbb themselves intact, the prior art approaches arerather limited in that they Sapply only to specific drugs in specific circumstances. With regard to the liposomes, which is probably the least invasive method to date to carry drugs across the bbb, there are a number of problems associated with them which have not been overcome by the prior art. Many of these prior art approaches display an unacceptable instability. For example, liposomes often exhibit severe stability problems and are therefore only of limited clinical use.

Thus, only liposomes so far are able to achieve improved bbb penetration of drugs. However, because of the well known disadvantages of instability and their S 'incompatibility with many drugs such as amphiphilic drugs and other agents, including many Sproteins and glycoproteins, their clinical use is severely compromised.

Rationale for this Patent C. Based on these considerations, a critical and long-felt need is apparent from the foregoing presentation for a method that allows drugs which do not pass the bbb S(hereafter referred to as "non-penetrating drugs") to become penetrable with features which overcome the disadvantages of the prior art devices, particularly the liposomes. In a similar scope, it is also desirable to improve the rate of penetration of drugs that normally do pass the bbb (hereafter referred to as "penetrating drugs") in order to reduce the peripheral side effects, while at the same time maintaining the desired effect(s) in the nervous system.

The subject of the present invention is a method, composition and drug S targeting system using surfactant coated nanoparticles as a drug carrier (or targeting S1- molecule) for a wide range of drugs in order to enhance the penetration of drugs ordiagnostic agents across the bbb.

Accordingly, it is an object of this invention to provide a method and composition for the administration of drugs affecting the nervous system to produce a Sphysiologic or pharmacologic effect, or to apply substances with diagnostic value, which overcomes the aforesaid disadvantages associated with the prior art methods and devices.

SStill another object of the present invention is to provide a method and omposition for allowing non-penetrating and penetrating drugs to pass the bbb more easily. -6- Yet another object of the invention is to provide for a reliable and easily used method and composition for treating disorders of the nervous system by systemic injection or oral application of drug-absorbed-nanoparticles.

A further object of the invention is to provide a method to increase drug transport when injected directly into the nervous system.

Finally, another object of the present invention is to provide a process for preparing the nanoparticles of the present invention.

These and other objects and features of the invention will be apparent from the S.detailed description and the drawings.

Summarv of the Invention The present invention features a method of delivering pharmacologically active substances across the blood-brain barrier and a drug targeting system useful for delivering drugs across the bbb. This invention is based on the surprising finding that i treatment of nanoparticles having a drug absorbed,adsorbed, or incorporated therein with a sufficient coating of an appropriate surfactant allows the adsorbed drug to traverse the bbb.

SWhile it is theorized that the nanoparticles cross the bbb and that the drug desorbs after transit of the nanoparticles, this step is not a necessary part of the invention so long as the drug traverses the bbb to yield its pharmacological action.. The term "pharmacologically active," S* as used herein, means and includes not just drug pharmaceutical activity but also diagnostic activity.

The basic drug targeting system is made by the following process. This Sprocess comprises: a. Formation of a suspension of nanoparticles by polymerization or dispersion b. Sorption of an active ingredient to the nanoparticle, and c. coating such nanoparticles with one or more layers of an appropriate surfactant.

More particularly, the method of the invention has the steps of loading a pharmacologically active substance such as a drug onto a nanoparticle, coating the loaded nanoparticle with a surfactant capable of directing the drug across the bbb, administering the j^ t 1 S: -7coated nanoparticles to a mammal in a.manner which allows the drug to reachand cross the bbb, and allowing the drug to be released from the nanoparticle to achieve the desired pharmacological effect. It is not clear if the nanoparticle itself crosses the bbb or whether only the drug crosses by being released from the nanoparticle.

However, the exact method is unimportant so long as the pharmacological effect is achieved.

The nanoparticle, which is a synthetic polymeric particle from about 1-1000 nm in diameter, is loaded with the drug by any known loading means. Commonly, solid nanoparticles are used and are loaded by sorption of the drug onto the surface of the nanoparticle, by soaking the preformed nanoparticle in a solution of the drug.

However, in some circumstances, the drug is added to the polymerization solution and the drug is incorporated into the nanoparticle as the nanoparticle is made. The critical, innovative step is that after drug absorption or incorporation, the nanoparticles are coated with surfactants by incubating them in a surfactant-solution under appropriate conditions. The surfactant allows penetration of the bbb by the drug without physical modification of the nanoparticle or the drug itself. A preferred surfactant is Polysorbate SThe critical and novel step of the process and composition of this invention is to monitor the time that is allowed for the surfactant to associate with a surface of the nanoparticles. Simply mixing is not sufficient to enable passage of the bbb by the drug. A major advantage of the system and method is that it can be used to transport .drugs which could not otherwise cross the bbb into the central nervous system or S could otherwise pass across the bbb into the central nervous system only in an amount being not or not sufficiently pharmacologically effictive.

The drug targeting system of the invention provides the means of carrying out the m 'ethod. This drug targeting system includes the drug-loaded nanoparticles which are coated with the appropriate surfactant, possibly carried in a suitable buffer or other physiologically acceptable carrier solution. The type of carrier, and its properties, t depend on how the nanoparticles are to be administered, orally, intravenously, intramuscularly or in so other manner. A very broad range of drugs can be delivered I in this system, and determining the optimum mode of targeting depends on the system selected.

-I Other objects, features and advantages of the invention will be apparent to those versed in the art from the detailed description of the specification which will now follow, taken in conjunction with the tables, drawings, and theaccompanying claims.

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The drawingsame not drawn to scale. They are set forth to illustrate various embodiments of the inventions and the results achieved, the drawings, to which reference will be made, =reas follows: FIGURE Ilis a schematic drawing of the nanoParticle, indicating its molecular structure. FIGURE 1 A displays a monolithic nanoparticle with drug dispersed or dissolved in matrixr and coated with a surfactant Figure 1B displays a capsule-type nanoparticle with drug entrapped in the interior with a surfactant coating. Figure lC displays a naparticle with surfa.ce-absorbed or -adsorbed drug v~rith an additional surfactant coating.

These three embodiments are not limiting because combinations thereof arm possible.

44Furthernmore. vafous numbers of coatings can be employed.

ect MPE)FIGURE 2 illustrates the analgesic effect in percent of maximally possible effec(MPE)in tail flick test after intravenous injection of dalargin (10,mg/kg). Dalargin was given either in solution (filled circles), in a simple mixture of drug, nanoparticles, and surfactant (open circle), or after sorptive binding to nanoparticles and coating with polysorbate 80 (at a dose of 7.5 mg dalargin/kg). The data, shown also in Table 1, wvere collected at different time points following injection.

4 FIGURE 3 is an electronmicrograph of nanopartictes in brain tissue. The *4 S graph clearly displays the nanoparticles in the capillary lumen., D~etaile DecitoLf h neto N~t should be understood that the detailed description and specific examples, while indicating preferred embodiments of the invention, are given by way of illustration only sice arius hange and modifications within the spirit and scope of the invention will become apparent to those skilled in the art from this description and the accompanying claims.

-9- What are iznopartides? The term "nanoparticie" as used herein denotes a carrier structure which is biocompatible with and sufficiently resistant to chemical and/or physical destruction by the environment of use such that a sufficient amount of the nanoparticles; remain substantially intact after injection into the blood stream, or given intraperitoneally or orally, so as to be able to reach the brain at the bbb. If the drug can cross the bbb in the form whereby it is Adsorbed to the nanoparticles, they must also remain sufficiently intact to cross the bbb.

Usually, nanoparticles are solid colloidal particles ranging in size from I to 1000 am. Drugs or other relevant materials these used for diagnostic purposes in nuclear medicine or in radiation therapy) can be dissolved within. the nanoparticles, entrapped, encapsulated and/or adsobed or attached.

General AattersRelating to the Fabrication efNanoparicfes- Nanoparticles Can be made from a broad number of materials includingacrylates, roethacrylates, methylmethacrylate-s, cyanoacrylates, acrylamides, polyacetates, polyglycolates, polyanhydrades, polyorthoesters, gelatin, polysaccharides, albumin, polystyrenes, pot)yvinyls. polyacroleines, polyglutataldehydes, and derivatives, copolymers, and derivatives thereof. Monomer materials particularly suitable to fabricate biodegradable nanoparticles by emulsion polymerization in a continuous aqueous phase include methylrnethacrylates, polyalkcyanoacrylates, nydroxyethiylmethcrlates, methacrylate acid, ethylene glycol dimethacrylate, acrylamide, N'bsehlnaxlmde ad2 dimethylaminoethyl methacrylate. Other nanoparticles are made by different techniques from N-L-lysinediylterephithalate, alkyr-yanoacrylate, polylactic acidplaci acidpolyglycolic acid-copolymer, polyanhydrates, polyorthoesters, gelatin, albumin, afid desolvated macromolecules or carbohydrates. Further, non-biodegradable materials can be used such apoyyeiie. poly (vinylpyridine),: polyacroleine and, polyglutaraldehyde. A summary of materials and fabrication methods for making nanoparticles has previously been pbished.SeKetr .(91 "Nanoparticles-prteparation and applications." In: M.

Donbrow 'Microcapsules and nanoparticles in medicine and pharmacy.' CRC press, Boca Ranton, Florida, pp.L 12-5-148 General Process of Fabrication Nanoparticles can be produced by convent-iona methods, including emulso polyerization in a continuous aqueous phase, emulsion polymerization in continuous organic phase. interffcial polymerization. solvent-deposition, solvent evaporation, t- ii r;l:i~iLi-: i. dissolvation of an organic polymer solution, cross-linking of water-soluble polymers in emulsion, dissolvation of macromolecules, and carbohydrate cross-linking. These fabrication methods can be performed with a wide range of polymer materials mentioned above.

The present invention teaches a process for preparation of coated nanoparticles which comprises: a. Formation of a suspension of nanoparticles, by polymerization or dispersion b. Sorption or incorporation of an active ingredient to the nanoparticle, and j c. coating such nanoparticles with one or more layers of an appropriate C C rsurfactant.

The drug or a diagnostic agent can either be adsorbed (or absorbed) to a premade nanoparticle or it can be incorporated into the nanoparticle during the manufacturing process. Methods of absorption, adsorption, and incorporation are common knowledge to ig ,those sliled in the art.

S Typical materials suitable for coating of the nanoparticles are surfactants S selected from a group comprising fatty acids, fatty acid esters of glycerols, sorbitol and S other multifunctional alcohols, as, for instance, glycerol monostearate, sorbitan monolaurate, or.

T Isorbitan monoleate; polysorbates, as, for instance, polysorbate 80 and polysorbate poloxamers, as, for instance, poloxamer 188, 338, or407; poloxamines, such as poloxamine 904 or 1508; polyoxyethylene ethers and polyoxyethylene esters; ethoxylated triglycerides; ethoxylated phenols and ethoxylated diphenols; surfactants of the Genapol TM and Bauki series; metal salts of fatty acids, metal salts of fatty alcohol sulfates, sodium lauryl sulfate; and metal salts of sulfosuccinates. Other surfactants are known which may be useful as coating materials for nanospheres have been described by H. Sucker et aL (Pharmazeutische STechrologie, George Thieme Verlag, 1978).

The choice of the monomer and/or polymer, the solvent, the emulsifier and the 1 i surfactant and other auxiliary substances will be dictated by the particular nanoparicle being .fabricated and can be chosen, without limitation and difficulty, by those skilled in the art.

S The limiting rquirement is that the combination allowspassage of the drug across the bbb.

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B.

-1l- The ratio of the drug to polymer can vary within a wide range. Also, the removal of the solvent or emulsifier can be achieved in a number of different ways.

Nanoparicles as Drug Carriers The biologically active ingredient (such as a drug) that can be suitably employed in accordance with the invention with warm blooded animals, particularly mammals including human, veterinarian animals, and farm animals, all are those affecting, acting on, or being visualized within the nervous system, including tumor tissue located therein. Also, the use of diagnostic agents is possible. There is essentially no limitation on the type of drug or other ingredient which may be used.

The present invention may be applied to deliver any agent for the treatment of disorders affecting the nervous system and it may also be applied for diagnostic purposes.

Preferred classes of agents for treatment of CNS disorders include: Drugs acting at synaptic and neuroeffector junctional sites; general and local analgesics and anesthetics such as opioid analgesics and antagonists; hypnotics and sedatives; drugs for the treatment of psychiatric disorders such as depression, schizophrenia; antiepileptics and anticonvulsants; Huntington's disease, aging and Alzheimer's disease; neuroprotective agents (such as excitatory amino acid antagonists and neurotropic factors) and neuroregenerative agents; trophic factors such as brain derived neurotrophic factor, ciliary neurotrophic factor, or nerve growth factor drugs aimed at the treatment of CNS trauma or stroke; and drugs for the treatment of addiction and drug abuse; autacoids and antiinflammatory drugs; chemotherapeutic agents for parasitic infections and microbial diseases; immuiosuppressive agents and anti-cancer drugs; hormones and hormone antagonists; heavy ietals and heavy.metal antagonists; antagonists for non-metallic toxic agents; cytostatic agents for the treatment of cancer; diagnostic substances for use in nuclear medicine, and radiation therapy immunoactive and immunoreactive agents; and a number of other agents such as transmitters and their respective receptor-agonists and -antagonists, their respective precursors or metabolites; antibitiics, aitispasmodics, antihistamines; antinauseants, relaxants, stimulants, "sense" and "anti-sense" oligonucleotides, cerebral delators, in o tottop strictors, ypertensives, mgrane psychotropics, nii-manics, vascular delators and constrictors, anti-hypertensives, mgram .treatments, hypnotics hyper- orhypglycemic agnts, mineral or nutritional agents, antiobeity drugs, anabolies ard anti-asthmatics.

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Typical active ingredients drugs) can be any substance affecting the nervous system or used for diagnostic tests of the nervous system. These are described by Gilman eta!f. (1990), "Goodman and Gilman's The Pharmacological Basis of Therapeutics", Perganon Press, New York, and include the following agents: acetylcholine and synthetic choline esters, naturally occurring cholinomimetic alkaloids and their synthetic congeners, antichoiniesterase agents, ganglioni .c stimulants, atropine, scopolamine and related antimuscarinic drugs, catecholarnines and sympat homimetiC drugs, such as epinephrine, norepinephrine and dopamine, adrenergic agonists, adrenergic receptor antagonists, transmitters such as GABA, glycine, glutamate, acetylcholine, dopamine, 5-hydroxytryptaiie, and histamine, neuroactive peptides; analgesics and anesthetics such as opioid analgesics and antagonists; preanesthetic and anesthetic medications such as benzodiazepines, barbiturates, antihistaminies, phenothiazines and butylphenones; opioids; antiemetics; anticholinergic drugs such as atropine, scopolamine or glycopyrrolate, ***e; chloral derivatives; ethchlorvynol; gluteihiniide; rnethyprylon; meprobamate; paraldehyde; disulfiram; morphine, featanyl and naloxone; centrally active antitussive agents; psychiatric drugs such as phenothiazines, thioxanthenes and other heterocyclic compounds halperiodol); tricyclic antidepressants such as desimipramine and imiprarnine; atypical antidepressants fluoxetine and trazodone), monoamine oxidase inhibitors such. as isocarboxazid; lithium salts; anxiolytics such as chlordiazepoxyd and diazepam; anti-epileptics including hydantoins, anticonvulsant barbiturates, iminostilbiries (such as carbarnazepine), succinimides, valproic acid, oxazolidinediones and benzodiazepines.

anti-Parkinson drugs such as L.DOPAICARBIDOPA, apomorphine, amatadine, ergolines, selegeline, rop .inorole, bromocriptine niesylate and anticholinergic agents, antispasticity agents such as baclofen, diazepamnand dantrolene; neuroprotedtiVe agents,'such as'excitatory amino acid antagonists, neu rotrophic factors -and brain derived neurotrophic factor. ciliary neurotrophic factor, or C I 3 -13nerve growth factor; neurotrophine(NT) 3 (NT3); NT4 and NTS; gangliosides; neuroregenerative agents; drugs for the treatment of addiction and drug abuse include opioid antagonists and anti-depressants; autocoids and anti-inflammatory drugs such as histamine, bradykinin, kallidin and their respective agonists and antagonists; chemotherapeutic agents for p a tinfections and microbial diseases; anti-cancer drugs including alkylating agents nitrosoureas) and antimetabolites; nitrogen mustards, ethylenamines and methylmelamines; alkylsulfonates; folic acid analogs; pyrimidine analogs, purine analogs, vinca alkaloids; and antibiotics.

The present invention is also useful for the delivery of anti-nauseants, relaxants, stimulants, "sense" and "anti-sense" oligonucleotides, cerebral delators, psychotropics, vascular delators and constrictors, anti-hypertensives, migraine treatments, hyper- or hypo-glycemic agents, mineral or nutritional agents, anti-obesity drugs, anabolics and anti-asthmatics, anti-inflammatory drugs such as phenylbutazone, indomethacin, naproxen, ibuprofen, flurbiprofen, diclofenac, dexamethasone, prednisone and prednisolone; cerebral vasodilators such as soloctidilum, vincamine, naftidrofuryl oxalate, co-dergocrine mesylate, cyclandelate, papaverine, nicotinic acid, anti-infective agents such as erythromycin Astearate, and cephalexin.

Mechanism ofBBB Transportfor Nanoparficles In accordance with the present invention, nanoparticles are able to carry (or deliver) drugs or diagnostics across the bbb. While not being bound by any particular theory, what comprises the mechanism of transport across the bbb and why it is noteworthy and unexpected is that it can not presently be explained by traditional concepts. At the present time, it is not possible to'showthe concrete mechanism of this peptide penetration across the bbb, although speculations can be made.

Banks eral. (1991) suggested some mechanisms of this peptide transport to the brain which may also apply to nanoparticles or materials carried by nanoparticles.

Transport can be achieved by nonsaturable and saturablemeans, as intact molecules or their metabolites. The degree of bbb passage depends primarily on lipid solubility of the molecule Banks, Kastin, "Peptides and blood-brain barrier- Lipophilicity as a predictor of C7 4CCJ -14permeability." Brain Res.Bull., 15:287-292(1985). Other factors that may influence brain entry are molecular weight, charge, degree of protein binding in the serum, although these seem to play a lesser role than lipophilicity (Banks et al., 1991). The transport mechanism suggested by Banks seems to be restricted to transporting a limited number of structurally related peptides such as met-encephalin and a few other closely related peptides. They do not apply, for instance, to p-endorphins and cyotorphines. Saturable transport rates are modulated by various factors, including some substances, like leucine and aluminum Banks, Kastin, "Editorial review: Peptide transport system for opiates across the bloodbrain barrier." Am. J. Physiol., 25:E1-E10(1990). Whether transport mechanisms of nanoparticles are similar to transport of peptides is not known currently. As the present invention is the first to demonstrate nanoparticle transport to the CNS ofa biologically active 'drug, no further information is available at present.

Specific Material and Process for Fabrication as Example Example 1 In the presently preferred embodiment, the nanoparticles are made of polyacyl cyanoacrylates (hereafter also referred to as "poly butylcyano acrylate") of the general formula: *0; 0:0 +0 00 0 *0 0 @00.

C0_CH 5 1, 4: SH 4"~ ii< 1 i1s:: B-4; :i In the preferred embodiment of the present invention, the nanoparticles were prepared using an acidic polymerization medium containing dextran 70000 as stabilizer (dextran 70000 1% in 0.1 N HCl). In the in vitrostudy, we used butyl cyanoacrylate which was added to obtain a 1% nanoparticle suspension. The mixture was agitated by stirring with amagnetic stirrer at 500 rpm for 4 h to allow nanoparticle formation. The resulting suspension was neutralized with 0.1 N sodium hydroxide solution, filtered through a sintered glass filter (pore size 10 pm), and 1%of anhydrous glucose was added to improve.

redispersability of thenanoparticles after lypholization. Particle size determination was done by means of photon correlation spectroscopy with a BO 20 Gniometer (Brookhaven Instr Corporation, Holtsville, York). An average diameter of 230 nm served. The nanoparticle suspension was then lyophiized using aLyovac GT 2 freeze dryer (Leybold AG K61n, Germany).

I

41 .I P L .i i }j 15 -15 Example2 S. S S S 5555 An alternative example of a method for nanoparticle fabrication with drug incorporation is the following: In this example, the nanoparticles are prepared using an acidic polnerization medium containing dextran 70000 as stabilizer (dextran 70000 1% in 0.2 N HC1) and 5 mg dalargin. In this in vitro study, we used butyl cyanoacrylate which was added to obtain a 1% nanoparticle suspension. The mixture was agitated by stirring with a magnetic stirrer at 500 rpm for 4 h to allow nanoparticle formation. The resulting suspension was neutralized with 0.1 N sodium hydroxide solution, filtered through a sintered glass filter (pore size 10 pm), and 1% of anhydrous glucose was added to improve redispersability of the nanoparticles after lypholization.

This example is yet another method for nanoparticle fabrication with drug sorption. Polylactic polyglycolic acid (PLGA) is dissolved in acetone (10 ml, 20.0 mg/ml) and a mixture of deionized water and ethanol is added dropwise (25G syringe needle) into the copolymer solution stirred by magnetic stirrer (Ika-Labortechnik, Germany), until turbidity indicative of copolymer precipitation is visually observed. The suspension of these preformed nanospheres is then added to an aqueous surfactant solution (15 ml, 1% w/v) placed in a glass beaker (50 ml) and agitated by a magnetic stirrer at ambient temperature until complete evaporation of the organic solvent has taken place.

Example 4 This example shows a method for albumin nanoparticle fabrication with drug sorption. Nanoparticles are prepared using a water in oil emulsification process as described in Widder, Flouret, and Senyei, "Magnetic microspheres: Synthesis ofa novel parenteral diug carrier." J. Pharm. Sci 7 9 -8 2 (19 7 9 One half ml of a 25% aqueous bovine serum albumin solution is mixed well with 30 ml ice-cooled cottonseed oil using a magnetic stirrer. The above emulsion is further subjected to ultrasonication (125 W, lh, Bransonic 220, Branson, Geneva, CH) while the system is kept ice-cooled. One hundred ml of cottonseed oil is then heated to 145"C I 0C (heating mantle 200 W/220 V, Heraeus- WittmannHeidelberg, GER) in a 500 ml three-necked round bottom flask (Schott, Mainz, Germany)while stirring is maintained at 1500 rpm (stirring motor type IKA, RW 18, Staufen i. Br. Gerany;stirring head MRKI NS 29/32 Buddeberg, Mannheim, Germany).

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1- 1 c~ Li~ *:i -s *i a^a 1-I tw -16- The preformed aqueous albumin in oil emulsion is added dropwise (100±10 drops/min.) into the preheated, rapidly stirred cottonseed oil through a needle tip (24G x 3/4 Terumo, Frankfurt, Germany) connected to a syringe (20 ml Luer, Braun Melsungen, Germany). Then the preformed albumin spheres are cross-linked by the maintenance of the heat. After ten minutes, the system is allowed to cool to room temperature, while stirring is maintained. The cooled mixture (25"C) is.then diluted with 100 nl of diethylether and -centrifuged at 2500 rpm (table centrifug- model GPR, Beckman, Minchen, Germany) for min. The supernatant is discarded and the washing procedure is repeated three times. After evaporation of the solvent, a free flowing powder is obtained which is stored at 4*C until use.

Another example of a method for albumin nanoparticle fabrication with drug sorption comprises the following steps: Albumin nanoparticles are produced by desolvation process according to a slightly modified method suggested in Marty, J.J. and Oppenheim, R.C. "Colloidal systems for drug delivery," Ausralian J. Pharm. Sc6:65-76(1977). Five hundred mg of albumin (BSA) is dissolved in 40 ml of purified water. About 60 ml of absolute ethanol is added, until the onset of protein desolvation can be visually observed by the rise in turbidity. The system is then cross-linked by addition of 0.1 ml glutaraldehyde and agitated for 1 hr. on a magnetic stirrer (KA, Heidelberg, Germany).

Unreacted glutaraldehyde is destroyed by carefully adding 0.5 ml of an aqueous 12% sodium metabisulfate solution. After a reaction time of another 3-4 hrs., excess ethanol is evaporated under vacuum. The obtained preparation is then further purified by column gel filtratiofi (Sephacryl G 1000, Pharmacia, Sweden). After the addition of 100 mg glucose, the resulting S particle suspension is lyophilized for about 16 hours (Lyovac, Heraeus, Hanau, Germany) in order to increase the redispersibilty of the product.

SThis example describes a series of experiments to show in vivo activity of the method of the invention. In the presently preferred embodiment f the invention for the in fvo experiment, thedrug dalargin was used to determine the usefulness of the current Sinvention an nanoparticles were prepared as described in example 1. The hexapeptide daargin is a leu-encephalinanalog which contains D-Ala insecond position in order to S: prevent enzymatic destruction (Tyr

D

Ala-Gly-PheLeiArg).

Generally, dalargin is used as a therapy for peripheral ulcers and from this S application t is kown that dalargin is stable in the blood stream. The injection of any of the ac: o i is kontown that dalc I m a 17-

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exhibits potent analgesic activity following intraventricular injection in the brain. However, it does not produce analgesia when given peripherally (Kalenikova et al., Farmakokinetika dalargina, Vopr. Med. Khim 34:75-8 3 (1 9 8 From this it can be concluded that dalargin, when administered into the blood stream, does not penetrate thcugh the bbb at all or in insufficient amounts to cause CNS action. As the present invention discloses, the appearance of analgesia after the peripheral injection of dalargin-adsorbed nanoparticles shows that nanoparticles are able to carry non-penetrating drugs across the bbb and thus serve as a novel drug transport method to the brain.

The following method is used to achieve "drug loading" of the nanoparticles.

The same procedure has also been found to work with the nanoparticles made using the procedures described in examples 3 through 5: One hundred mg of the lyophilized nanoparticles were resuspended in 5 ml phosphate buffered saline (PBS), bisodium phosphate/monobasic potassium phosphatelsodium chloride (7.6/1.45/4.8 w/w/w) containing 0.09% of dalargin. The peptide was allowed to absorb to the nanoparticle surface for three hours. Total amount ofthe peptide absorbed was calculated by filtering the suspension through a membrane filter of 10 nm pore size (Minisart; Sartorius AG G6ttingen, Germany) and measuring the amount of free peptide in the filtrate by means of UV spectrophotometry at 220 nm wavelength: It was shown that 30% of the peptide (1.35 mg) was absorbed to the nanoparticles. The suspension was diluted in PBS to obtain a peptide concentration between 0.25 and 0.75 mg/ml and sonicated for five minutes. After that, the nanoparticles were coated with an appropriate surfactant.

While many coating materials can be used to achieve the desired effect, in the presently preferred embodiment.the following coating materials were used: poloxamers 184, 188, 338,407 (POE-POP-blockcopolymers obtained from C.H. Erbsloeh, Disseldorf, Germany), poloaxamine 908 (ethylenediamine POE-POP-blockcopolymer, C.H. Erbesloeh), polysorbates 20 and 80 (Atlas Chemie, Essen, Germany), and Brij 35 (polyethylene 23 lauryl ether, Fluka, Buchs,

CH).

To achieve the coating of the drug-absorbed nanoparticles, 1% of surfactant was added to the nanoparticle suspension, incubated for 30 min. and immediately thereafter injected into mice. It is possible to vary the suspension time and the concentration of the surfactant in suspension. All of the surfactants coated the nanoparticles appropriately.

Proof of Concept To evaluate the biological activity of the drug after absorbing it to naoparicles and cating them with surfacant, an in viv assay was used.

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1; 9* In order to test the pharmacological usefulness of this approach,,we absorbed the nanoparticles with a drug which does not pass the bbb when given systemically, namely, the len-encephalin analog dalargin. Dalargin is ahighly potent analgesic when injected directly into the brain, but it is without any effect when given peripherally. Dalargin-was absorbed to poly (butyl cyanoacrylate) nanoparticles and incubated in an aqueous Polysorbate 81 solution for 30 min. After this time, this preparation was injected intravenously into mice at dalargin dosages of 2.5, 5.0, and 7.5 mg/kg. Various preparations include pure dalargin solution, uncoated dalargin nanoparticles, a freshly prepared mixture of nanoparticles, drug, and surfactant without allowing drug or surfactant sorption times as well as pure surfactant or nanoparticle solutions served as controls. Activity threshold was measured with the tail flick test Dalargin, when dissolved in PBS up to a dose of 10 mg/kg, did not exhibit any analgesic effect after i.v. injection (Fig. In fact, only dalargin absorbed to nanoparticles and coated with Polysorbate 80 had an analgesic activity which became statistically significant at a dose of 5 mg/kg dalargin as indicated by the tail flick test. All other preparations including those containing dalargin up to a dose of 10 mg/kg had no analgesic effect at all. To conduct the proper control experiments, we included the following groups in our studies: Group 1: suspension of empty nanoparticles (200mg/kg).

Group Polysorbate 80 solution in PBS.

Group 3: dalargin solution in PBS.

S Group 4: mixture ofdalarginsolution and Polysorbate 5 Group5: mixture of dalarginsolution and empty nanoparticles.

S Group 6: mixture of dalargin, empty particles and Polysorbate 80 after mixing of the drug and surfactant with the particles without any equilibration time.

Group 7: dalargin loaded by incubating for 3 hrs. onto empty nanoparticles and injected without Polysorbate Groups 8-10 dalgrin loaded nanoparticles (2.5,5.0 and 7.5 mg/kg, Srespectively) with the Polysorbate 80 coating.

Group 11 daligrin loaded nanoparticles (7.5 mg/kg) with Polysorbate c oating.

Group 12 daligrin loaded nanoparticles (75 mg/kg) with Poloxamine 908 coating.

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thm.Ofpatcuarinees wic ls ~tosowanlgsc ff9tI The results are displayed in Table I and in Figure 2. None of the control groups (Groups 1-7) exhibited any analgesic effects in tece which wer ijctdwh a a *6.

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Group 1: suspensioni ofen-pt nopries (200 mg/kg-) Gop2:. Polysorbate 80 solution in PBS Group 3: alrgin solution in PBS GroupA4:. mixture of dalargin solution andPojyso rbate Grup: mxueof dalargin'solution and empty nnoparticles Group 6, mixture of dalargin, empty particles and'Polysorbate .80 aflerMixinig of the-drug and sur Ifactant with the particles withouit any equilibration time Group 7: da Ilargin loaded by incubating or 3 hi-S. onito empty nanoparticles and injeted, without Polysorbate 80 coating Group S: polysorbate 80-coated and dalargini-loaded nanoparticles (2.5 mg/kg) Group 9: Polysorbate 80-coated and dalargin-loaded nanoparticles (5.0 mg/kg) Group 10: Polysorbat'e 80-coated and dalargin-loadd nanoparticles (7.5 mg/kg) 20 Group I1 Polysorbate 20-coated and dalargin-loaded nanoparticles (7.5 mg/kg) Group 12 Polyoxamine 908-coated and dalargin-loaded nanoparticles (7.5 mg/kg) The specificity of the analgesic effect in the brain was documented by application of an opioid antagonist. Nalaxone (0.1 mg/kg) diminished the effectiveness of dalargin bound to Polysorbate 80-coated nanoparticles To determine the fateof the nanoparticles, histological investigations were Sconducted with fluorescein-loaded Polysorbate 80-coated nanoparticles. These studies indicate that the coated nanoparticles were taken up by the endothelial cells lining the brain S blood capillaries and seem to be released later into the interior brain compartment (See Fig. 3 and Fig. 4).

Taken together, these results indicate that a drug, when bound to the appropriately coated nanoparticle, shows a biological effect in the brain (in this specific case Sleading to analgesia). This is due to a previously impossible passage of the drug through the bbb which could be achieved by one or more of the following mechanisms: enhancement of S" the transport of the drug through the bbb by diffusion or by an activation of endocytotic uptake by endothelial cells of the brain blood vessels.

Theoretically, there are some possibilities to influence the penetration of drugs 1 through the bbb either by the use of active transport or by passive ways.

4 Polysorbate 80 is a very interesting substance in this respect for brain targeting -and enhancement of the uptake of some substances. Troster, Muller, Kreuter, J., "Modification of the body distribution of poly (methyl methacrylate) nanoparticles in rats by coating with surfactants. Int. J. Pharm. 1:85-100(1990), demonstrated an increaed Saccumulation ofnanoparticle radioactivity in the brain area after injection of polysorbate 14C-poly(methyl methacrylate) nanoparticles. However, the same paper also showed similar uptake with other surfactantsin the bran. Since these polymers are only very slowlybiodagradable, this accumulation within the time frame of the mentioned study has to be due to intact particles.

SHowever, as mentioned before andas shown in Figure 2 and Table 1, the simple mixture of nanparticles with surfactants as used in the Trister study did not lead to :any rpt of the drug across the bbb. In an earlier study by Kreuter, Hartmann,

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"C omparative study on the cytosttic effects and te tisue distribution of5-fluorouracil in a free form and bound to polybutyanoarylate nanopaticlesin sarcoma 180-bearing ce.

Oncoloy :363-366(1 an enhanced 5-fluorouracil accumulation into the brain was

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4 -21observed in comparison to a free solution of the drug after using nanoparticles prepared in a Polysorbate 20-containing medium. At that time, this result did not attract any attention, since the binding to nanoparticles induced an increased 5-fluorouracil concentration in all organs investigated. In addition, the same situation as in the Tr6ster study likely occurred in that the particles accumulated in the blood stream of the brain without crossing the bbb. As mentioned above, the induction of dalargin activity in the present invention was possible only after binding to nanoparticles and only after attainment of an equilibrium binding of the drug.

Mixing of this drug, Polysorbate 80 and the nanoparticles and the i.v. injection immediately after mixing exhibited no drug action at all. This clearly demonstrates that the activity was only due to drug bound to intact particles.

The mechanism of the transport induction could be due to a number of mechanisms. First, nanoparticles may be bound to the inner endothelial lining of the brain capillaries. Subsequently, the nanoparticles would just deliver the drug more efficiently to the brain cells by providing a large concentration gradient and simple diffusion. The second possibility is brain endothelial uptake by phagocytes. As we have shown in the in vitro study above, Polysorbate 80 induces an increased tissue uptake of nanoparticles in brain blood vessel endothelium. Again, the drug could then be delivered by diffusion out of the endothelial cells to the brain cells. Alternatively, but probably less likely, the nanoparticles with the drugs could be exocytosed into the surrounding brain tissue.

The possibility exists that Polysorbate 80; moreover, seems to have bbbopening properties. Sakaneer al. (1989) showed that a 6% solution ofPolysorbate provided an enhanced passage of insulin and the dipeptide b-kyotorphin through the bbb in the brain. However, with the in vivo experiment above, we have clearly shown that this can be ruled out as a possible mechanism. Because group 4 (mixture of dalargin solution and Polysorbate 80) did not show analgesia on the tail flick test, the Polysorbate alone does not result in dalargin passage. Thus, our method provides a specific bbb passage method which clearly displays an unexpected improvement over the prior art. The mechanism is not one of nondiscriminant opening of the bbb itself toa CNS-active drug.

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Claims (5)

1. 22. The claims defining the invention are as follows: I C II It S. a 5,. 4R 4 1- Drug targeting system for administration to a innmal comprising nanoparticles made of a polymeric material, said nanoparticles compri sing a drug to be delivered to said mammal and a surfactant, co. ating deposited thereon; and a physiologically acceptable carrier andfor diluent. allowing the transport of sad nanoparticles to the target within said mamnmal after a dministratio n 2. Drug targetinig system according to claim 1, wherein, said nanoparticles cmrs synthetic polymeric particles having a diameter in the range of from I to 1.000 nm. 3. Drug targeting system according to claim I or claim 2, wherein said synthetic polymeric nanoparticles are formed of a .polymer selected from the group consistn of .acrylates. meihacrylates, cyanoacrylates, acrylamidesi polylactates.Polyglycolates, polyanhydratcs, pol .yorthoesters, gelatin, albumin, polystYrees, polYvinyls, polyacrolein, polyglutaraldehyd and dervvs Copolymlersanmitrsteof 4-Du ageigsse according to any of the claims I to 3, wherein said hnaoparticles comprise the drug to be delivered in a fr bobd dobdado in~corporated thereto. I 23 Drug targeting system according to any of the claims 1 to 4, wherein said surfactant is selected from the group consisting of surfactants which allow passage of said nanoparticles through the blood-brain barrier in said mammal and those which allow release of said drug from said nanoparticles and transit of the blood- brain barrier by said drug separate from said nanoparticles. 6. Drug targeting system according to claim 5, wherein said surfactant is one which allows transit of the blood-brain barrier by said drug without chemical modification of said drug. 1 7. Drug targeting system according to any of the claims 1 to 6 wherein said surfactant comprises polysorbate S8. Drug targeting system according to any of the claims 1 to 7, wherein said drug comprises a substance which has central nervous system activity but cannot cross the blood-brain carrier without modification or without a carrier. 9. Drug targeting system according to any of the claims 1 to 8, wherein said drug is selected from the group consisting of drugs acting at synaptic and neuroeffector S junctional sites; general and local analgesics and anethetics; hypnotics and sedatives; Sdrugs forthe treatmentofpsychiatric disorders such as depression and schizophrenia; anti-epileptics and anticovulsants; drugs for treating Huningon's disease, ing and Alzheimer's disease; excitatory ai acidantagonists and neurotropic factors and Sneuroregenerativ agents; trophic factors; drugs aimed at the treatment of CNS Strauma or stroke drugs for te treatment of addictionand drug abuse; autacoids and anti-inflammatory drugs; chemotherapetic .agents for parasitic infections and Smicrobial diseases; immunosuppressve agents and anti-cancer drugs; hormones and hormoneantagonists; heavy metals and heavy metal antagonists; antagonists for non- metallic toxic agents; cytostatic agents for the treatmentof cancer diagnosti subsances for use in nuclear medicine, immuoactive and immunoreactive agents; ansmiers and their respective eceptor-aonissand -antagonists their espective AL 24 precursors or metabolites; antibiotics, antispasmodics, antihistarnmins, antinauseants, relaxants, stimulants, "sense" and "anti-sense" oligonucleotides, cerebral delators, psychotropics, anti-manics, vascular delators and constrictors, anti-hypeitensives, migraine treatments, hypnotics, hyper- or hypo-glycemic agents, mineral or nutritional agents, anti-obesity drugs, anabolics and anti-asthmatics, and mixtures thereof. Drug targeting system according to any of the claims 1 to 8, wherein said drug comprises a diagnostic Rgent. 11. Drug targeting system according to any of the claims l to 10, wherein said carrier and/or dilucnt is selected from the group consisting of water, physiologically acceptable aqueous solutions containing salts and/or buffers or any other solution acceptable for mammalian treatment. 12 A method for peparing a drug targeting system for administration of one or -more pharmacologically active substance(s) to a mammal, said method comprising the steps of preparing nanoparticles by suitably polymerizing a polymer-generating monomeric or oligomeric material; oading said pharmacologically active substaice(s) onto or into said nano- particles; coating said loaded nanoparticles with a surfactant; and S optionally providing said loaded and coated nanoparticles in a medium allowing the transport of said nanoparticles t he target within said mammal after administration. 13. The method accordijg to claim 12, wherein said polymerization step is carried out by a -method selected from the group consisting of emulsion polymerization, interfacial polymerization, solvent deposition, solvent evaporation, and cross-linking of olers in solution t I i 4:. i.-r:1: 14. The method according to claim 12 or claim 13, wherein the polymer formed by said polymerization step and forming said nanoparticles is selected from the group consisting of acrylates, methacrylates, cyanoacrylates, acrylamides, polylactates, polyglycolates, polyanhydrates, polyorthoesters, gelatin, albumin, polystyrenes, polyvinyls, polyacrolein, polyglutaraldehyde and derivatives, copolymers and mixtures thereof. The method according to any of the claims 12 to 14, wherein said loading step comprises incorporation of said pharmacologically active substance into said I c I c nanoparticles by manufacturing said nanoparticles in the presence of said pharmaco- I o t -logically active substance. 16. The method according to any of the claims 12 to 14, wherein said loading step comprises mixing said pharmacologically active substance and said nanoparticles in solution and allowing sufficient time for an effective amount of said pharmacologi- cally active substance to be adsorbed and/or absorbed by said nanoparticles. 1 7. The method according to any of the claims 12 to 16, wherein said coating step S comprises mixing said loaded nanpaticles with a solution of said surfactant and allowing sufficient time for said surfactat to coat said nanoparticles 18. Theiethodaccording to claim 17, wherein said srfactant is selected from the group onsisting of surfactants which allow pssage ofsaid nanparticle through the blood-brain barrier in said mammal and those wich allowrelease of said drug from said nanoparticles and transit of the blood-brain barrier by said drug separate from said nanoparticle:s. A: i Th:: acrdig tocaim 18, ,wh:eiad: sufactn riso~ o which allows. ta-sit- o thiet bloo-min^ br~r iryid- dr-- o l ec lod- cto of drug.~ 26 The method according to any of the claims 12 to 19, wherein said surfactant comprises polysorbate 21. The method according to any of the claims 12 to 20, wherein said pharmacologi- cally active substance comprises a drug, preferably a drug selected from the group consisting of drugs acting at synaptic and neuroeffector junctional sites; general and local analgesics and anesthetics; hypnotics and sedatives; drugs for the treatment of psychiatric disorders such as depression and schizophrenia; anti-epileptics and anticonvulsants; drugs for treating Huntington's disease, aging and Alzheimer's .1 disease; excitatory amino acid antagonists and neurotropic factors and neuroregenera- tive agents; trophic factors; drugs aimed at the treatment of CNS trauma or stroke; drugs for the treatment of addiction and drug abuse; autacoids and anti-inflammatory drugs; chemotherapeutic agents for parasitic infections and microbial diseases; immunosuppressive agents and anti-cancer drugs; hormones and hormone ant- S agonists; heavy metals and heavy metal antagonists; antagonists for non-metallic toxic agents; cytostatic agents for the treatment of cancer; diagnostic substances for Suse in nuclear medicine, immunoactive and immunoreactive agents; transmitters and their respective receptor-agonists and -antagonists, their respective precursors or metabolites; antibiotics, antispasmodics, antihistamines, antinauseants, relaxants, -stimulants, "sense" and "anti-sense" oligonucleotides, erebral delators, psychotro- S pics, anti-manics, vascular delators and constrctors, anti-hypertensives, migraine treatments, hypnotics, hyper- or h ypo mc agents, mineral or nutritional agents; anti-obesity drugi, anabolics and anti-asthmatics, and mixtures thereof. anti-o'berty A -asth *'thereof. 22. The method according to any ofthe claims 12 to20, wherein said pharmacologi- cally active substance comrises a diagnostic agentpreferably one which is useful in the diagnosis for nuclear medicine and radiation therapy.
2. 23. The method according to any of the claims 12 to 22, wherein said medium allowing the transport of said naparticles to the target within said mammal afte j^ 27 S administration is selected from the group consisting of water, physiologically acceptable aqueous solutions containing salts and/or buffers, or any other solution acceptable for mammalian treatment.
3. 24. The drug targeting system according to any of the claims 1 to 11 for medical use. S 25. The drug targeting system according to any of the claims 1 to 11 for use for delivering one or more pharmacologically active substance(s) across the blood-brain barrier of a mammal, preferably of a human.
4. 26. Use of the drug targeting system according to any of the claims 1 to 11 for manufacturing a medicament for achieving a pharmacological effect in the central nervous system of a mammal. S 27. Use according to claim 26 for manufacturing a medicament for achieving a pharmacological effect in the central nervous system of a mammal by the action of Spha acolgicall active substance otherwise not passing across theblood-brain t c barrier..
5. 28. Use according to claim 26 for manufacturing a medicament for achieving a 1 28. Use 9ar^ to^ pharmacological effect in the central nervous system of a mammal bythe action of a pharmacologically active substance otherwise passing across the blood-brain barrier only in an amount being not or not sufficiently pharmacologically effective. 1 29 Use according to any of the claims 26 to 28 for manufacturing a medicament for an oral or intravenous administration. Use according to any of the claims 26 to 29,- wherein said mammal is a human. I i: i:i i 5 i.. Dated this 21 "L day of December 1998 MEDINOVA MEDICAL CONSULTING GmbH By their Patent Attorneys CULLEN CO. C (C cC c CL C *1 K It j~ej .1 t I J 7