AU8147498A - Alpha 1a adrenergic receptor antagonists - Google Patents

Description

WO 98/57632 PCT/US98/12573 TITLE OF THE INVENTION ALPHA la ADRENERGIC RECEPTOR ANTAGONISTS This application claims the benefit of U.S. Provisional 5 Application No. 60/050,136, filed June 18, 1997. FIELD OF THE INVENTION This invention relates to certain novel compounds and derivatives thereof, their synthesis, and their use as alpha la 10 adrenoceptor antagonists. More particularly, the compounds of the present invention are useful for treating benign prostatic hyperplasia (BPH). BACKGROUND OF THE INVENTION 15 Human adrenergic receptors are integral membrane proteins which have been classified into two broad classes, the alpha and the beta adrenergic receptors. Both types mediate the action of the peripheral sympathetic nervous system upon binding of catecholamines, norepinephrine and epinephrine. 20 Norepinephrine is produced by adrenergic nerve endings, while epinephrine is produced by the adrenal medulla. The binding affinity of adrenergic receptors for these compounds forms one basis of the classification: alpha receptors bind norepinephrine more strongly than epinephrine and much more strongly than the synthetic compound 25 isoproterenol. The binding affinity of these hormones is reversed for the beta receptors. In many tissues, the functional responses, such as smooth muscle contraction, induced by alpha receptor activation are opposed to responses induced by beta receptor binding. Subsequently, the functional distinction between alpha and 30 beta receptors was further highlighted and refined by the pharmacological characterization of these receptors from various animal and tissue sources. As a result, alpha and beta adrenergic receptors were further subdivided into alpha 1, alpha 2, B1, and 12 subtypes. Functional differences between alpha 1 and alpha 2 receptors WO 98/57632 PCT/US98/12573 have been recognized, and compounds which exhibit selective binding between these two subtypes have been developed. For a general background on the alpha adrenergic receptors, the reader's attention is directed to Robert R. Ruffolo, Jr., a 5 Adrenoreceptors: Molecular BioloLv. Biochemistry and Pharmacology, (Progress in Basic and Clinical Pharmacolovgy series, Karger, 1991), wherein the basis of alpha 1/alpha 2 subclassification, the molecular biology, signal transduction (G-protein interaction and location of the significant site for this and ligand binding activity away from the 3' 10 terminus of alpha adrenergic receptors), agonist structure-activity relationships, receptor functions, and therapeutic applications for compounds exhibiting alpha-adrenergic receptor affinity was explored. The cloning, sequencing and expression of alpha receptor subtypes from animal tissues has led to the subclassification of the 15 alpha 1 receptors into alpha ld (formerly known as alpha la or la/ld), alpha lb and alpha la (formerly known as alpha 1c) subtypes. Each alpha 1 receptor subtype exhibits its own pharmacologic and tissue specificities. The designation "alpha la" is the appellation recently approved by the IUPHAR Nomenclature Committee for the previously 20 designated "alpha 1c" cloned subtype as outlined in the 1995 Receptor and Ion Channel Nomenclature Supplement (Watson and Girdlestone, 1995). The designation alpha la is used throughout this application to refer to this subtype. At the same time, the receptor formerly designated alpha la was renamed alpha ld. The new nomenclature is used 25 throughout this application. Stable cell lines expressing these alpha 1 receptor subtypes are referred to herein; however, these cell lines were deposited with the American Type Culture Collection (ATCC) under the old nomenclature. For a review of the classification of alpha 1 adrenoceptor subtypes, see, Martin C. Michel, et al., Naunyn 30 Schmiedeberg's Arch. Pharmacol. (1995) 352:1-10. The differences in the alpha adrenergic receptor subtypes have relevance in pathophysiologic conditions. Benign prostatic hyperplasia, also known as benign prostatic hypertrophy or BPH, is an illness typically affecting men over fifty years of age, increasing in 35 severity with increasing age. The symptoms of the condition include, -2- WO 98/57632 PCT/US98/12573 but are not limited to, increased difficulty in urination and sexual dysfunction. These symptoms are induced by enlargement, or hyperplasia, of the prostate gland. As the prostate increases in size, it impinges on free-flow of fluids through the male urethra. 5 Concommitantly, the increased noradrenergic innervation of the enlarged prostate leads to an increased adrenergic tone of the bladder neck and urethra, further restricting the flow of urine through the urethra. In benign prostatic hyperplasia, the male hormone 5alpha 10 dihydrotestosterone has been identified as the principal culprit. The continual production of 5(-dihydrotestosterone by the male testes induces incremental growth of the prostate gland throughout the life of the male. Beyond the age of about fifty years, in many men, this enlarged gland begins to obstruct the urethra with the pathologic 15 symptoms noted above. The elucidation of the mechanism summarized above has resulted in the recent development of effective agents to control, and in many cases reverse, the pernicious advance of BPH. In the forefront of these agents is Merck & Co., Inc.s' product PROSCAR® (finasteride). 20 The effect of this compound is to inhibit the enzyme testosterone 5a reductase, which converts testosterone into 5a-dihydrotesterone, resulting in a reduced rate of prostatic enlargement, and often reduction in prostatic mass. The development of such agents as PROSCAR® bodes well 25 for the long-term control of BPH. However, as may be appreciated from the lengthy development of the syndrome, its reversal also is not immediate. In the interim, those males suffering with BPH continue to suffer, and may in fact lose hope that the agents are working sufficiently rapidly. 30 In response to this problem, one solution is to identify pharmaceutically active compounds which complement slower-acting therapeutics by providing acute relief. Agents which induce relaxation of the lower urinary tract tissue, by binding to alpha 1 adrenergic receptors, thus reducing the increased adrenergic tone due to the 35 disease, would be good candidates for this activity. Thus, one such agent -3- WO 98/57632 PCT/US98/12573 is alfuzosin, which is reported in EP 0 204597 to induce urination in cases of prostatic hyperplasia. Likewise, in WO 92/0073, the selective ability of the R(+) enantiomer of terazosin to bind to adrenergic receptors of the alphal subtype was reported. In addition, in WO 92/161213, 5 combinations of 5a-reductase inhibitory compounds and alphal adrenergic receptor blockers (terazosin, doxazosin, prazosin, bunazosin, indoramin, alfuzosin) were disclosed. However, no information as to the alpha ld, alpha lb, or alpha la subtype specificity of these compounds was provided as this data and its relevancy to the treatment of BPH was 10 not known. Current therapy for BPH uses existing non-selective alpha 1 antagonists such as prazosin (Minipress, Pfizer), Terazosin (Hytrin, Abbott) or doxazosin mesylate (Cardura, Pfizer). These non-selective antagonists suffer from side effects related to antagonism of the alpha ld and alpha lb receptors in the peripheral vasculature, e.g., hypotension 15 and syncope. The recent cloning of the human alpha la adrenergic receptor (ATCC CRL 11140) and the use of a screening assay utilizing the cloned human alpha la receptor enables identification of compounds which specifically interact with the human alpha la adrenergic 20 receptor. [PCT International Application Publication Nos. W094/08040, published 14 April 1994 and WO94/10989, published 26 May 1994] As disclosed in the instant patent disclosure, a cloned human alpha la adrenergic receptor and a method for identifying compounds which bind the human alpha la receptor has now made possible the identification of 25 selective human alpha la adrenergic receptor antagonists useful for treating BPH. The instant patent disclosure discloses novel compounds which selectively bind to the human alpha la receptor. These compounds are further tested for binding to other human alpha 1 receptor subtypes, as well as counterscreened against other types of 30 receptors (e.g., alpha 2), thus defining the specificity of the compounds of the present invention for the human alpha la adrenergic receptor. It is an object of the present invention to identify compounds which bind to the alpha la adrenergic receptor. It is a further object of the invention to identify compounds which act as antagonists of the 35 alpha la adrenergic receptor. It is another object of the invention to -4- WO 98/57632 PCT/US98/12573 identify alpha la adrenergic receptor antagonist compounds which are useful agents for treating BPH in animals, preferably mammals, especially humans. Still another object of the invention is to identify alpha la adrenergic receptor antagonists which are useful for relaxing 5 lower urinary tract tissue in animals, preferably mammals, especially humans. It has now been found that the compounds of the present invention are alpha la adrenergic receptor antagonists. Thus, the compounds of the present invention are useful for treating BPH in 10 mammals. Additionally, it has been found that the alpha la adrenergic receptor antagonists of the present invention are also useful for relaxing lower urinary tract tissue in mammals. SUMMARY OF THE INVENTION 15 The present invention provides compounds for the treatment of urinary obstruction caused by benign prostatic hyperplasia (BPH). The compounds antagonize the human alpha la adrenergic receptor at nanomolar and subnanomolar concentrations while exhibiting at least ten fold lower affinity for the alpha ld and alpha lb 20 human adrenergic receptors and many other G-protein coupled receptors. This invention has the advantage over non-selective alpha 1 adrenoceptor antagonists of reduced side effects related to peripheral adrenergic blockade. Such side effects include hypotension, syncope, lethargy, etc. The compounds of the present invention have the 25 structure: n R__ N Q R ;J

R

3

R

6 G L wherein Q is selected from -5- WO 98/57632 PCT/US98/12573 (X) q (X)q K- K , R o , (X)q R1 1 R 1 0 0 7 R9 R 13,a R7 O R" 12 ,,N ON NV (X),q R j W 0 0 N w H , O or -(X) C1-8 alkyl, C3-8 cycloalkyl, (CH2)0-40R 15, (CH2)0-4N(R16)2, (CH2)0-4CN, (CH2)0-4CF3 , (CH2)0-4CO2R 16, (CH2)0-4CON(R16)2, 1 0 Is (CH2)04SO2R 15 or (CH2)0-4SO2N(R16)2; or E, G, L and M are each independently selected from hydrogen, C1-8 alkyl, C3-8 cycloalkyl, (CH1-8 alkyR 1 5 , C3-8 cycloalkyl-4N(R16)2, (CH2)-4CN, (CH2)0-4N(R 6 )2CF3, 5 (CH2)O-4CN, (CH2)O-4CF3, (CH2)0-4C02Rl 6 , (CH2)O-4CON(Rl 6 )2, (CH2)O-4S02Rl 5 or (CH2)QA4SO2N(Rl 6 )2; J is selected from hydrogen, C1-8 alkyl, C3-8 cycloalkyl, (CH2)1-40R 1 5 , (CH2)l-4N(Rl 6 )2, (CH2)1-4CN, (CH2)0-4CF3, 10 (CH2)0-4CO2R 1 6 , (CH2)0-4CON(R 1 6 )2, (CH2)0-4SO2R 1 5 or (CH2)0-4SO2N(R 1 6 )2;

R

1 is selected from unsubstituted, mono- or poly-substituted phenyl wherein the substitutents on the phenyl are independently selected from -6- WO 98/57632 PCT/US98/12573 halogen, CF3, cyano, nitro, N(R 1 6 )2, NR 1 6COR 1 8 , NR 1 6

CON(R

1 8 )2,

NR

1 6 SO2R 1 8 , NR 16 SO2N(R 1 8 )2, OR 1 5 , (CH2)0-4CO2R 16 , (CH2)0-4CON(R 1 6 )2, (CH2)0-4SO2N(R 1 6 )2, (CH2)0-4SO2R 1 5 or C1-4 alkyl; or unsubstituted, mono- or poly-substituted pyridyl, 5 pyrazinyl, thienyl, thiazolyl, furanyl, quinazolinyl or naphthyl wherein the substituents on the pyridyl, pyrazinyl, thienyl, thiazolyl, furanyl, quinazolinyl or naphthyl are independently selected from CF3, cyano, nitro, N(R 1 6 )2, (CH2)0-4CO2R 1 6 , (CH2)0-4CON(R 1 6 )2, (CH2)0-4SO2N(R 1 6 )2, (CH2)0-4SO2R 1 5 , phenyl, OR 1 5 , halogen, 10 C1-4 alkyl or 3-8 cycloalkyl; R is selected from hydrogen, cyano, OR 15 , CO2R 1 5 , CON(R 1 6 )2, SO2R 1 5 , SO2N(R 1 6 )2, tetrazole, isooxadiazole, unsubstituted, mono- or poly substituted phenyl wherein the substitutents on the phenyl are 15 independently selected from halogen, cyano, OR 1 5 , (CH2)0-4CO2R 1 6 , (CH2)0-4CON(R1 6 )2, N(R 1 6 )2, NR 1 6

COR

1 5 , NR 1 6

CON(R

1 8 )2,

NR

1 6 SO2R 1 5 , NR 1 6 SO2N(R 1 8 )2, (CH2)0-4SO2N(R 1 6 )2, (CH2)0-4SO2R 15 or C1-4 alkyl; or unsubstituted, mono- or poly substituted pyridyl, thienyl, furanyl or naphthyl wherein the 20 substituents on the pyridyl, thienyl, furanyl or naphthyl are independently selected from CF3, (CH2)0-4CO2R 1 6 , (CH2)0-4CON(R 1 6 )2, (CH2)0-4SO2N(R 1 6 )2, (CH2)0-4SO2R 1 5 , phenyl, OR 1 5 , halogen, C1-4 alkyl or C3-8 cycloalkyl; 25 R 2 and R 7 are each independently selected from hydrogen, C1-8 alkyl, C4-8 cycloalkyl, (CH2)0-4CO2R 1 6 , (CH2)0-4CON(R 1 6 )2, (CH2)0-4COR 15 , (CH2)2-4OR 1 5 , (CH2)1-4CF3, (CH2)0-4SO2R 15 , (CH2)0-4SO2N(R 16 )2 or (CH2)1-4CN; 30 R 3 , R 6 , R 8 , R 9 and R 1 0 are each independently selected from hydrogen, C1-8 alkyl, C3-8 cycloalkyl, (CH2)1-4OR 1 5 or (CH2)0-4CF3;

R

1 1 and R 1 2 are each independently selected from hydrogen, -7- WO 98/57632 PCT/US98/12573 C1-8 alkyl or C3-8 cycloalkyl;

R

1 3 and R 1 4 are each independently selected from hydrogen, 01-8 alkyl, C3-8 cycloalkyl, (CH2)0-4OR 1 5 , (CH2)0-4CF3, unsubstituted, mono- or 5 poly-substituted phenyl wherein the substitutents on the phenyl are independently selected from halogen, CF3, cyano, nitro, CO2R 1 6 , OR 1 5 , (CH2)0-4CON(R1 6 )2, (CH2)0-4CO2R 1 6 or C1-4 alkyl; or unsubstituted, mono- or poly-substituted: pyridyl, thienyl, furanyl or naphthyl wherein the substituents on the pyridyl, thienyl, 10 furanyl or naphthyl are independently selected from CF3, phenyl, OR 1 5 , halogen, C1-4 alkyl or C3-8 cycloalkyl;

R

1 5 is selected from hydrogen, C1-8 alkyl, C3-8 cycloalkyl or (CH2)0-4CF3; 15

R

1 6 and R 1 8 are each independently selected from hydrogen, C1-8 alkyl, C3-8 cycloalkyl or (CH2)1-4CF3;

R

1 9 is selected from hydrogen, C1-8 alkyl, C3-8 cycloalkyl, 20 (CH2)0-4OR 15 or (CH2)0-4CF3; W is O or NR 1 1 ; each X is independently selected from halogen, cyano, nitro, C1-8 alkyl, 25 C3-8 cycloalkyl, (CH2)0-.40R 2 4 or (CH2)0-4CF3;

R

24 is selected from hydrogen, C1.8 alkyl, C3-8 cycloalkyl or (CH 2 )04CF 3 ; Y is C-R 1 5 or N; 30 Z is hydrogen, oxygen or sulphur; m, n, p and q are each independently an integer from zero to four; o is an integer from one to four; -8- WO 98/57632 PCT/US98/12573 r is zero or one; and the pharmaceutically acceptable salts thereof. In one embodiment of the invention is the compound of the 5 formula M n E R2 R 3 Q R3 G L wherein E, G, L, M and J are each independently selected from hydrogen, C1-8 alkyl, C3-8 cycloalkyl, (CH2)0-4CO2R 1 6 or (CH2)0-4CF3; 10

R

1 is selected from unsubstituted, mono-, di- or tri-substituted phenyl wherein the substitutents on the phenyl are independently selected from halogen, CF3, cyano, nitro, N(R 1 6 )2, NR 1 6

COR

1 8 , NR 1 6

CON(R

1 8 )2,

NR

16 SO2R 1 8 , NR 1 6 SO2N(R 1 8 )2, OR 1 5 , (CH2)0-4CO2R 1 6 , 15 (CH2)0-4CON(R 1 6 )2, (CH2)0-4SO2N(R 1 6 )2, (CH2)0-4SO2(R 1 5 )2 or C1-4 alkyl; or unsubstituted, mono-, di- or tri-substituted pyridyl, pyrazinyl, thienyl, thiazolyl, furanyl, quinazolinyl or naphthyl wherein the substituents on the pyridyl, pyrazinyl, thienyl, thiazolyl, furanyl, quinazolinyl or naphthyl are independently selected from CF3, cyano, 20 nitro, N(R 1 6 )2, (CH2)0-4CO2R 1 6 , (CH2)0-4CON(R 16 )2, (CH2)0-4SO2N(R 1 6 )2, (CH2)0-4SO2(R 1 5 )2, phenyl, OR 1 5 , halogen, C1-4 alkyl or C3-8 cycloalkyl; R is selected from hydrogen, cyano, OR 1 5 , C02R 1 5 , CON(R 1 6 )2, S02R 1 5 , 25 SO2N(R 16 )2 or unsubstituted, mono- or di-substituted phenyl wherein the substitutents on the phenyl are independently selected from halogen, cyano, OR 1 5 , (CH2)0-4CO2R 1 6 , (CH2)0-4CON(R 1 6 )2, N(R 1 6 )2,

NR

1 6

COR

1 5 , NR 1 6

CON(R

1 8 )2, NR 1 6 SO2R 1 5 , NR 1 6 SO2N(R 18 )2, (CH2)0 4SO2N(R 1 6 )2, (CH2)0-4SO2R 1 5 or - 9- WO 98/57632 PCT/US98/12573 C1-4 alkyl; or unsubstituted, mono- or di-substituted pyridyl, thienyl, furanyl or naphthyl wherein the substituents on the pyridyl, thienyl, furanyl or naphthyl are independently selected from CF3, (CH2)0-4CO2R 1 6 , (CH2)0-4CON(R 1 6 )2, (CH2)0-4SO2N(R 1 6 )2, (CH2)0 5 4SO2R 1 5 , phenyl, OR 1 5 , halogen, C1-4 alkyl or C3-8 cycloalkyl;

R

2 and R 7 are each independently selected from hydrogen, C1-8 alkyl, C4-8 cycloalkyl or (CH2)1-4CF3; 10

R

1 3 and R 1 4 are each independently selected from hydrogen, C1-8 alkyl, C3-8 cycloalkyl, (CH2)1-40R 1 5 , (CH2)0-4CF3, unsubstituted, mono-, di- or tri-substituted phenyl wherein the substitutents on the phenyl are independently selected from halogen, CF3, cyano, nitro, 15 CO2R 16 , OR 1 5 , (CH2)0-4CON(R 1 6 )2, (CH2)0-4CO2R 1 6 or C1-4 alkyl; or unsubstituted, mono-, di- or tri substituted: pyridyl, thienyl, furanyl or naphthyl wherein the substituents on the pyridyl, thienyl, furanyl or naphthyl are independently selected from CF3, phenyl, OR 1 5 , halogen, C1-4 alkyl or 20 C3-8 cycloalkyl; n is an integer from zero to two; o is an integer from one to four; and all other variables are as defined previously; 25 and the pharmaceutically acceptable salts thereof. In a class of the invention is the compound of the formula S E

R

2 R N-(CH 2 )6-Q wherein Q is selected from - 10- WO 98/57632 PCT/US98/12573 (X)q 7 R19 R13 R~~ ~~~ o.7./- ~ x N N R R9 0 (X (X 0 or 0 0 E and J are each independently selected from hydrogen or CO2-C1-6 alkyl; 5 R 1 is selected from unsubstituted, mono-, di- or tri-substituted phenyl wherein the substitutents on the phenyl are independently selected from halogen, CF3, cyano, nitro, N(R 1 6 )2, OR 1 5 , (CH2)0-2CO2R 1 6 , (CH2)0-2CON(R1 6 )2 or C1-4 alkyl; or unsubstituted, mono- or di substituted pyridyl wherein the substitutents on the pyridyl are 10 independently selected from halogen, CF3, cyano, nitro, N(R 1 6 )2, OR 1 5 , (CH2)0-2CO2R 1 6 , (CH2)0-2CON(R 1 6 )2 or C1-4 alkyl; R is selected from hydrogen, cyano, OR 1 5 , CO2R 1 5 , CON(R 1 6 )2, SO2R 1 5 or SO2N(R 1 6 )2; 15

R

2 and R 7 are each independently selected from hydrogen, C1-6 alkyl, C4-6 cycloalkyl or (CH2)1-4CF3;

R

8 , R 9 and R 1 0 are each independently selected from hydrogen, 20 01-6 alkyl, C3-6 cycloalkyl, (CH2)2-4OR 1 5 or (CH2)0-2CF3;

R

1 3 is selected from hydrogen, C1-6 alkyl, C3-6 cycloalkyl, - 11- WO 98/57632 PCT/US98/12573 (CH2)2-40R 1 5 , (CH2)0-2CF3, or unsubstituted, mono-, or di- substituted phenyl wherein the substitutents on the phenyl are independently selected from halogen, CF3, cyano, CO2R 1 6 , OR 1 5 or C1-4 alkyl; 5 R 1 5 is selected from hydrogen, C1-6 alkyl, C3-6 cycloalkyl or (CH2)0-2CF3;

R

1 6 is selected from hydrogen, C1-6 alkyl, C3-6 cycloalkyl or (CH2)1-2CF3; 10

R

1 9 is selected from hydrogen, C1-6 alkyl, C3-6 cycloalkyl, (CH2)0-40R 1 5 or (CH2)0-2CF3; each X is independently selected from halogen or C1-4 alkyl, 15 p is an integer from zero to two; q is an integer from zero to three; and all other variables are as defined above; 20 and the pharmaceutically acceptable salts thereof. In a subclass of the inention is the compound of the formula R H

(R

17 )s R wherein Q is N N HO 0 -0 25 A is C-R 1 7 or N; - 12- WO 98/57632 PCT/US98/12573 R is selected from hydrogen, cyano, hydroxy, CO2R 1 5 , CON(R 1 6 )2, S02R 1 5 or SO2N(R 1 6 )2; 5 each R 1 7 is independently selected from hydrogen, halogen, C02R 1 6 , cyano, nitro, CON(R 1 6 )2, S02R 1 5 , SO2N(R 1 6 )2 or OR 1 5 ; each X is independently selected from flourine or methyl; 10 s is an integer from zero to two; and all other variables are as defined above; and the pharmaceutically acceptable salts thereof. Illustrative of the invention is the compound selected from 15 (4-cyano-4-phenyl-cyclohexyl)-[3-(2,2-di-p-tolyl-acetylamino)-propyl] methyl-ammonium chloride; (4-cyano-4-phenyl-cyclohexyl)-methyl-[4-(1,1,3-trioxo-1,3-dihydro-116 20 benzo[d]isothiazol-2-yl)-butyl]-ammonium chloride; (4-cyano-4-phenyl-cyclohexyl)-methyl-[3-(1,1,3-trioxo-1,3-dihydro-116 benzo[d]isothiazol-2-yl)-propyl]-ammonium chloride; 25 (+)-2-Oxo-4-(3,4,5-trifluorophenyl)-oxazolidine-3-carboxylic acid [2-(4-cyano-4-phenyl-cyclohexylamino)-ethyl] amide; (+)-2-Oxo-4-(3,4-difluorophenyl)-oxazolidine-3-carboxylic acid [2-(4-phenyl-4-methoxycarbonyl-cyclohexylamino)-ethyl] amide; 30 (+)-cis-2-Oxo-4-(3,4-difluorophenyl)-oxazolidine-3-carboxylic acid [2-(4-Cyano-4-(2-ethoxyphenyl)-cyclohexylamino)-ethyl] amide; [4-cyano-4-(2-methoxy-phenyl)-cyclohexyl]-(2-{[4-(3,4-difluoro-phenyl) -2 35 oxo-oxazolidine-3-carbonyl]-amino}-ethyl)-ammonium chloride; - 13- WO 98/57632 PCT/US98/12573 [4-cyano-4-(2-fluoro-phenyl)-cyclohexyl]-(2-{[4-(3,4-difluoro-phenyl)-2-oxo oxazolidine-3-carbonyl]-amino}-ethyl)-ammonium chloride; 5 4-(3,4-difluoro-phenyl)-2-oxo-oxazolidine-3-carboxylic acid {2-[4-cyano-4 (2-trifluoromethoxy-phenyl)-cyclohexylamino]-ethyl}-amide; 4-(3,4-difluoro-phenyl)-2-oxo-oxazolidine-3-carboxylic acid {2-[4-cyano-4 (2-trifluoromethoxy-phenyl)-cyclohexylamino]-ethyl}-amide; 10 4-(3,4-difluoro-phenyl)-2-oxo-oxazolidine-3-carboxylic acid {2-[4-cyano-4 (2-trifluoromethyl-phenyl)-cyclohexylamino]-ethyl}-amide; 4-(3,4-difluoro-phenyl)-2-oxo-oxazolidine-3-carboxylic acid {2-[4-cyano-4 15 (2-trifluoromethyl-phenyl)-cyclohexylamino]-ethyl}-amide; (2- {[4-(3,4-difluoro-phenyl)-2-oxo-oxazolidine-3-carbonyl]-amino}-ethyl) (4-methanesulfonyl-4-phenyl-cyclohexyl)-ammonium chloride; or 20 (2- {[4-(3,4-difluoro-phenyl)-2-oxo-oxazolidine-3-carbonyl]-amino}-ethyl) (4-methanesulfonyl-4-phenyl-cyclohexyl)-ammonium chloride and the pharmaceutically acceptable salts thereof. An illustration of the invention is a pharmaceutical 25 composition comprising a therapeutically effective amount of any of the compounds described above and a pharmaceutically acceptable carrier. An example of the invention is a pharmaceutical composition made by combining any of the compounds described above and a pharmaceutically acceptable carrier. Another illustration of the 30 invention is a process for making a pharmaceutical composition comprising combining any of the compounds described above and a pharmaceutically acceptable carrier. Exemplifying the invention is the composition further comprising a therapeutically effective amount of a testosterone 5-alpha 35 reductase inhibitor. Preferably, the testosterone 5-alpha reductase - 14- WO 98/57632 PCT/US98/12573 inhibitor is a type 1, a type 2, both a type 1 and a type 2 (i.e., a three component combination comprising any of the compounds described above combined with both a type 1 testosterone 5-alpha reductase inhibitor and a type 2 testosterone 5-alpha reductase inhibitor) or a dual 5 type 1 and type 2 testosterone 5-alpha reductase inhibitor. More preferably, the testosterone 5-alpha reductase inhibitor is a type 2 testosterone 5-alpha reductase inhibitor. Most preferably, the testosterone 5-alpha reductase inhibitor is finasteride. More specifically illustrating the invention is a method of 10 treating benign prostatic hyperplasia in a subject in need thereof which comprises administering to the subject a therapeutically effective amount of any of the compounds (or any of the compositions) described above. Further exemplifying the invention is the method of 15 treating BPH wherein the compound (or composition) additionally does not cause a fall in blood pressure at dosages effective to alleviate BPH. Another example of the invention is the method of treating benign prostatic hyperplasia wherein the compound is administered in combination with a testosterone 5-alpha reductase inhibitor. Preferably, 20 the testosterone 5-alpha reductase inhibitor is finasteride. Further illustrating the invention is a method of inhibiting contraction of prostate tissue or relaxing lower urinary tract tissue in a subject in need thereof which comprises administering to the subject a therapeutically effective amount of any of the compounds (or any of the 25 compositions) described above. More specifically exemplifying the invention is the method of inhibiting contraction of prostate tissue or relaxing lower urinary tract tissue wherein the compound (or composition) additionally does not cause a fall in blood pressures at dosages effective to inhibit contraction 30 of prostate tissue. More particularly illustrating the invention is the method of inhibiting contraction of prostate tissue or relaxing lower urinary tract tissue wherein the compound (or composition) is administered in combination with a testosterone 5-alpha reductase inhibitor; preferably, 35 the testosterone 5-alpha reductase inhibitor is finasteride. - 15- WO 98/57632 PCT/US98/12573 More particularly exemplifying the invention is a method of treating a disease which is susceptible to treatment by antagonism of the alpha la receptor which comprises administering to a subject in need thereof an amount of any of the compounds described above effective to 5 treat the disease. Diseases which are susceptible to treatment by antagonism of the alpha la receptor include, but are not limited to, BPH, high intraocular pressure, high cholesterol, impotency, sympathetically mediated pain, migraine (see, K.A. Vatz, Headache 1997:37: 107-108) and cardiac arrhythmia. 10 An additional illustration of the invention is the use of any of the compounds described above in the preparation of a medicament for: a) the treatment of benign prostatic hyperplasia; b) relaxing lower urinary tract tissue; or c) inhibiting contraction of prostate tissue; in a subject in need thereof. 15 An additional example of the invention is the use of any of the alpha la antagonist compounds described above and a 5-alpha reductase inhibitor for the manufacture of a medicament for: a) treating benign prostatic hyperplasia; b) relaxing lower urinary tract tissue; or c) inhibiting contraction of prostate tissue which comprises an effective 20 amount of the alpha la antagonist compound and an effective amount of 5-alpha reductase inhibitor, together or separately. DETAILED DESCRIPTION OF THE INVENTION Representative compounds of the present invention exhibit 25 high selectivity for the human alpha la adrenergic receptor. One implication of this selectivity is that these compounds display selectivity for lowering intraurethral pressure without substantially affecting diastolic blood pressure. Representative compounds of this invention display 30 submicromolar affinity for the human alpha la adrenergic receptor subtype while displaying at least ten-fold lower affinity for the human alpha ld and alpha lb adrenergic receptor subtypes, and many other G protein coupled human receptors. Particular representative compounds of this invention exhibit nanomolar and subnanomolar affinity for the 35 human alpha la adrenergic receptor subtype while displaying at least 30 - 16- WO 98/57632 PCT/US98/12573 fold lower affinity for the human alpha ld and alpha lb adrenergic receptor subtypes, and many other G-protein coupled human receptors (e.g., serotonin, dopamine, alpha 2 adrenergic, beta adrenergic or muscarinic receptors). 5 These compounds are administered in dosages effective to antagonize the alpha la receptor where such treatment is needed, as in BPH. For use in medicine, the salts of the compounds of this invention refer to non-toxic "pharmaceutically acceptable salts." Other salts may, however, be useful in the preparation of the compounds according to the 10 invention or of their pharmaceutically acceptable salts. Suitable pharmaceutically acceptable salts of the compounds of this invention include acid addition salts which may, for example, be formed by mixing a solution of the compound according to the invention with a solution of a pharmaceutically acceptable acid such as hydrochloric acid, sulphuric 15 acid, fumaric acid, maleic acid, succinic acid, acetic acid, benzoic acid, citric acid, tartaric acid, carbonic acid or phosphoric acid. Furthermore, where the compounds of the invention carry an acidic moiety, suitable pharmaceutically acceptable salts thereof may include alkali metal salts, e.g. sodium or potassium salts; alkaline earth metal 20 salts, e.g. calcium or magnesium salts; and salts formed with suitable organic ligands, e.g. quaternary ammonium salts. Thus, representative pharmaceutically acceptable salts include the following: Acetate, Benzenesulfonate, Benzoate, Bicarbonate, Bisulfate, Bitartrate, Borate, Bromide, Calcium, Camsylate, Carbonate, 25 Chloride, Clavulanate, Citrate, Dihydrochloride, Edetate, Edisylate, Estolate, Esylate, Fumarate, Gluceptate, Gluconate, Glutamate, Glycollylarsanilate, Hexylresorcinate, Hydrabamine, Hydrobromide, Hydrochloride, Hydroxynaphthoate, Iodide, Isothionate, Lactate, Lactobionate, Laurate, Malate, Maleate, Mandelate, Mesylate, 30 Methylbromide, Methylnitrate, Methylsulfate, Mucate, Napsylate, Nitrate, N-methylglucamine ammonium salt, Oleate, Pamoate (Embonate), Palmitate, Pantothenate, Phosphate/diphosphate, Polygalacturonate, Salicylate, Stearate, Sulfate, Subacetate, Succinate, Tannate, Tartrate, Teoclate, Tosylate, Triethiodide and Valerate. - 17- WO 98/57632 PCT/US98/12573 Compounds of this invention are used to reduce the acute symptoms of BPH. Thus, compounds of this invention may be used alone or in conjunction with a more long-term anti-BPH therapeutics, such as testosterone 5a-reductase inhibitors, including PROSCAR® 5 (finasteride). Aside from their utility as anti-BPH agents, these compounds may be used to induce highly tissue-specific, localized alpha la adrenergic receptor blockade whenever this is desired. Effects of this blockade include reduction of intra-ocular pressure, control of cardiac arrhythmias, and possibly a host of alpha la receptor mediated central 10 nervous system events. The present invention includes within its scope prodrugs of the compounds of this invention. In general, such prodrugs will be functional derivatives of the compounds of this invention which are readily convertible in vivo into the required compound. Thus, in the 15 methods of treatment of the present invention, the term "administering" shall encompass the treatment of the various conditions described with the compound specifically disclosed or with a compound which may not be specifically disclosed, but which converts to the specified compound in vivo after administration to the patient Conventional procedures for the 20 selection and preparation of suitable prodrug derivatives are described, for example, in "Design of Prodrugs," ed. H. Bundgaard, Elsevier, 1985. Metabolites of these compounds include active species produced upon introduction of compounds of this invention into the biological milieu. Where the compounds according to the invention have at 25 least one chiral center, they may accordingly exist as enantiomers. Where the compounds according to the invention possess two or more chiral centers, they may additionally exist as diastereoisomers. It is to be understood that all such isomers and mixtures thereof are encompassed within the scope of the present invention. Furthermore, 30 some of the crystalline forms for compounds of the present invention may exist as polymorphs and as such are intended to be included in the present invention. In addition, some of the compounds of the present invention may form solvates with water (i.e., hydrates) or common organic solvents. Such solvates are also encompassed within the scope 35 of this invention. - 18- WO 98/57632 PCT/US98/12573 The term "alkyl" shall mean straight or branched chain alkanes of one to ten total carbon atoms, or any number within this range (i.e., methyl, ethyl, 1-propyl, 2-propyl, n-butyl, s-butyl, t-butyl, etc.). 5 The term "alkenyl" shall mean straight or branched chain alkenes of two to ten total carbon atoms, or any number within this range. The term "aryl" as used herein, except where otherwise specifically defined, refers to unsubstituted, mono- or poly-substituted 10 aromatic groups such as phenyl or naphthyl. The term "cycloalkyl" shall mean cyclic rings of alkanes of three to eight total carbon atoms (i.e., cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl or cyclooctyl). Whenever the term "alkyl" or "aryl" or either of their prefix 15 roots appear in a name of a substituent (e.g., aralkoxyaryloxy) it shall be interpreted as including those limitations given above for "alkyl" and "aryl." Designated numbers of carbon atoms (e.g., C1-10) shall refer independently to the number of carbon atoms in an alkyl or cyclic alkyl moiety or to the alkyl portion of a larger substituent in which alkyl 20 appears as its prefix root. The term "halogen" shall include iodine, bromine, chlorine and fluorine. The term "substituted" shall be deemed to include multiple degrees of substitution by a named substitutent. The term "poly 25 substituted" as used herein shall include di-, tri-, tetra- and penta substitution by a named substituent. It is intended that the definition of any substituent or variable (e.g., X, R 1 6 , R 1 8 ) at a particular location in a molecule be independent of its definitions elsewhere in that molecule. Thus, 30 N(R 1 6 )2 represents -NH2, -NHCH3, -NHC2H5, -N(CH3)C2H5, etc. It is understood that substituents and substitution patterns on the compounds of the instant invention can be selected by one of ordinary skill in the art to provide compounds that are chemically stable and that can be readily synthesized by techniques known in the art as well as 35 those methods set forth below. - 19- WO 98/57632 PCT/US98/12573 Where multiple substituent moieties are disclosed or claimed, the substituted compound can be independently substituted by one or more of the disclosed or claimed substituent moieties, singly or plurally. 5 The term "Z is hydrogen," when refering to the "Q" group R13 Z NO R ' refers to the moiety

R

13 N

R

8 The term heterocycle or heterocyclic ring, as used herein, 10 represents an unsubstituted or substituted stable 5- to 7-membered monocyclic ring system which may be saturated or unsaturated, and which consists of carbon atoms and from one to three heteroatoms selected from N, O or S, and wherein the nitrogen and sulfur heteroatoms may optionally be oxidized, and the nitrogen heteroatom 15 may optionally be quaternized. The heterocyclic ring may be attached at any heteroatom or carbon atom which results in the creation of a stable structure. Examples of such heterocyclic groups include, but is not limited to, piperidinyl, piperazinyl, oxopiperazinyl, oxopiperidinyl, oxopyrrolidinyl, oxoazepinyl, azepinyl, pyrrolyl, pyrrolidinyl, furanyl, 20 thienyl, pyrazolyl, pyrazolidinyl, imidazolyl, imidazolinyl, imidazolidinyl, pyridyl, pyrazinyl, pyrimidinyl, pyridazinyl, oxazolyl, oxazolidinyl, isooxazolyl, isoxazolidinyl, morpholinyl, thiazolyl, thiazolidinyl, isothiazolyl, thiadiazolyl, tetrahydropyranyl, thiamorpholinyl, thiamorpholinyl sulfoxide, thiamorpholinyl sulfone, 25 and oxadiazolyl. Morpholino is the same as morpholinyl. - 20- WO 98/57632 PCT/US98/12573 The term "(S)-oxa" as used herein, refers to an oxazolidinone group of the formula (X)q 08 R9 N 0 R1io 0 O for example, F F 0 A N 5 O The term "activated (S)-oxa" as used herein, refers to an N-(activated)carbamate of the desired oxazolidinone where the activating group is, for example, a p-nitrophenyloxy group. A specific example of an activated (S)-oxa group is 4-(3,4-difluorophenyl)-2-oxo-oxazolidine-3 10 carboxylic acid 4-nitrophenyl ester (i.e., compound 2). The term "selective alpha la adrenergic receptor antagonist," as used herein, refers to an alpha la antagonist compound which is at least ten fold selective for the human alpha la adrenergic receptor as compared to the human alpha lb, alpha ld, alpha 2a, alpha 15 2b and alpha 2c adrenergic receptors. The term "lower urinary tract tissue," as used herein, refers to and includes, but is not limited to, prostatic smooth muscle, the prostatic capsule, the urethra and the bladder neck. - 21- WO 98/57632 PCT/US98/12573 The term "subject," as used herein refers to an animal, preferably a mammal, most preferably a human, who has been the object of treatment, observation or experiment. The term "therapeutically effective amount" as used 5 herein means that amount of active compound or pharmaceutical agent that elicits the biological or medicinal response in a tissue, system, animal or human that is being sought by a researcher, veterinarian, medical doctor or other clinician, which includes alleviation of the symptoms of the disease being treated. 10 The present invention also provides pharmaceutical compositions comprising one or more compounds of this invention in association with a pharmaceutically acceptable carrier. Preferably these compositions are in unit dosage forms such as tablets, pills, capsules, powders, granules, sterile parenteral solutions or 15 suspensions, metered aerosol or liquid sprays, drops, ampoules, auto injector devices or suppositories; for oral, parenteral, intranasal, sublingual or rectal administration, or for administration by inhalation or insufflation. Alternatively, the compositions may be presented in a form suitable for once-weekly or once-monthly administration; for 20 example, an insoluble salt of the active compound, such as the decanoate salt, may be adapted to provide a depot preparation for intramuscular injection. For preparing solid compositions such as tablets, the principal active ingredient is mixed with a pharmaceutical carrier, e.g. conventional tableting ingredients such as corn starch, lactose, sucrose, 25 sorbitol, talc, stearic acid, magnesium stearate, dicalcium phosphate or gums, and other pharmaceutical diluents, e.g. water, to form a solid preformulation composition containing a homogeneous mixture of a compound of the present invention, or a pharmaceutically acceptable salt thereof. When referring to these preformulation compositions as 30 homogeneous, it is meant that the active ingredient is dispersed evenly throughout the composition so that the composition may be readily subdivided into equally effective unit dosage forms such as tablets, pills and capsules. This solid preformulation composition is then subdivided into unit dosage forms of the type described above containing from 0.1 to 35 about 500 mg of the active ingredient of the present invention. The - 22- WO 98/57632 PCT/US98/12573 tablets or pills of the novel composition can be coated or otherwise compounded to provide a dosage form affording the advantage of prolonged action. For example, the tablet or pill can comprise an inner dosage and an outer dosage component, the latter being in the form of an 5 envelope over the former. The two components can be separated by an enteric layer which serves to resist disintegration in the stomach and permits the inner component to pass intact into the duodenum or to be delayed in release. A variety of materials can be used for such enteric layers or coatings, such materials including a number of polymeric 10 acids and mixtures of polymeric acids with such materials as shellac, cetyl alcohol and cellulose acetate. As used herein, the term "composition" is intended to encompass a product comprising the specified ingredients in the specified amounts, as well as any product which results, directly or 15 indirectly, from combination of the specified ingredients in the specified amounts. The liquid forms in which the novel compositions of the present invention may be incorporated for administration orally or by injection include aqueous solutions, suitably flavoured syrups, aqueous 20 or oil suspensions, and flavoured emulsions with edible oils such as cottonseed oil, sesame oil, coconut oil or peanut oil, as well as elixirs and similar pharmaceutical vehicles. Suitable dispersing or suspending agents for aqueous suspensions include synthetic and natural gums such as tragacanth, acacia, alginate, dextran, sodium 25 carboxymethylcellulose, methylcellulose, polyvinyl-pyrrolidone or gelatin. Where the processes for the preparation of the compounds according to the invention give rise to mixtures of stereoisomers, these isomers may be separated by conventional techniques such as 30 preparative chromatography. The compounds may be prepared in racemic form, or individual enantiomers may be prepared either by enantiospecific synthesis or by resolution. The compounds may, for example, be resolved into their component enantiomers by standard techniques, such as the formation of diastereomeric pairs by salt 35 formation with an optically active acid, such as (-)-di-p-toluoyl-d-tartaric - 23- WO 98/57632 PCT/US98/12573 acid and/or (+)-di-p-toluoyl-l-tartaric acid followed by fractional crystallization and regeneration of the free base. The compounds may also be resolved by formation of diastereomeric esters or amides, followed by chromatographic separation and removal of the chiral 5 auxiliary. Alternatively, the compounds may be resolved using a chiral HPLC column. During any of the processes for preparation of the compounds of the present invention, it may be necessary and/or desirable to protect sensitive or reactive groups on any of the molecules 10 concerned. This may be achieved by means of conventional protecting groups, such as those described in Protective Groups in Organic Chemistry, ed. J.F.W. McOmie, Plenum Press, 1973; and T.W. Greene & P.G.M. Wuts, Protective Groups in Organic Synthesis, John Wiley & Sons, 1991. The protecting groups may be removed at a convenient 15 subsequent stage using methods known from the art. The specificity of binding of compounds showing affinity for the alpha la receptor is shown by comparing affinity to membranes obtained from tranfected cell lines that express the alpha la receptor and membranes from cell lines or tissues known to express other types of 20 alpha (e.g., alpha ld, alpha lb) or beta adrenergic receptors. Expression of the cloned human alpha ld, alpha lb, and alpha la receptors and comparison of their binding properties with known selective antagonists provides a rational way for selection of compounds and discovery of new compounds with predictable pharmacological activities. Antagonism by 25 these compounds of the human alpha la adrenergic receptor subtype may be functionally demonstrated in anesthetized animals. These compounds may be used to increase urine flow without exhibiting hypotensive effects. The ability of compounds of the present invention to 30 specifically bind to the alpha la receptor makes them useful for the treatment of BPH. The specificity of binding of compounds showing affinity for the alpha la receptor is compared against the binding affinities to other types of alpha or beta adrenergic receptors. The human alpha adrenergic receptor of the la subtype was recently 35 identified, cloned and expressed as described in PCT International -24- WO 98/57632 PCT/US98/12573 Application Publication Nos. WO94/08040, published 14 April 1994 and WO 94/21660, published 29 September 1994. The cloned human alpha la receptor, when expressed in mammalian cell lines, is used to discover ligands that bind to the receptor and alter its function. Expression of the 5 cloned human alpha ld, alpha lb, and alpha la receptors and comparison of their binding properties with known selective antagonists provides a rational way for selection of compounds and discovery of new compounds with predictable pharmacological activities. Compounds of this invention exhibiting human alpha la 10 adrenergic receptor antagonism may further be defined by counterscreening. This is accomplished according to methods known in the art using other receptors responsible for mediating diverse biological functions. [See e.., PCT International Application Publication No. WO94/10989, published 26 May 1994; U.S. Patent No. 5,403,847, issued 15 April 4, 1995]. Compounds which are both selective amongst the various human alphal adrenergic receptor subtypes and which have low affinity for other receptors, such as the alpha2 adrenergic receptors, the 3 adrenergic receptors, the muscarinic receptors, the serotonin receptors, and others are particularly preferred. The absence of these non-specific 20 activities may be confirmed by using cloned and expressed receptors in an analogous fashion to the method disclosed herein for identifying compounds which have high affinity for the various human alphal adrenergic receptors. Furthermore, functional biological tests are used to confirm the effects of identified compounds as alpha la adrenergic 25 receptor antagonists. The present invention also has the objective of providing suitable topical, oral, systemic and parenteral pharmaceutical formulations for use in the novel methods of treatment of the present invention. The compositions containing compounds of this invention 30 as the active ingredient for use in the specific antagonism of human alpha la adrenergic receptors can be administered in a wide variety of therapeutic dosage forms in conventional vehicles for systemic administration. For example, the compounds can be administered in such oral dosage forms as tablets, capsules (each including timed 35 release and sustained release formulations), pills, powders, - 25- WO 98/57632 PCT/US98/12573 granules, elixirs, tinctures, solutions, suspensions, syrups and emulsions, or by injection. Likewise, they may also be administered in intravenous (both bolus and infusion), intraperitoneal, subcutaneous, topical with or without occlusion, or intramuscular 5 form, all using forms well known to those of ordinary skill in the pharmaceutical arts. An effective but non-toxic amount of the compound desired can be employed as an alpha la antagonistic agent. Advantageously, compounds of the present invention 10 may be administered in a single daily dose, or the total daily dosage may be administered in divided doses of two, three or four times daily. Furthermore, compounds for the present invention can be administered in intranasal form via topical use of suitable intranasal vehicles, or via transdermal routes, using those forms of 15 transdermal skin patches well known to those of ordinary skill in that art. To be administered in the form of a transdermal delivery system, the dosage administration will, of course, be continuous rather than intermittent throughout the dosage regimen. The dosage regimen utilizing the compounds of the 20 present invention is selected in accordance with a variety of factors including type, species, age, weight, sex and medical condition of the patient; the severity of the condition to be treated; the route of administration; the renal and hepatic function of the patient; and the particular compound thereof employed. A physician or veterinarian 25 of ordinary skill can readily determine and prescribe the effective amount of the drug required to prevent, counter or arrest the progress of the condition. Optimal precision in achieving concentration of drug within the range that yields efficacy without toxicity requires a regimen based on the kinetics of the drug's 30 availability to target sites. This involves a consideration of the distribution, equilibrium, and elimination of a drug. In the methods of the present invention, the compounds herein described in detail can form the active ingredient, and are typically administered in admixture with suitable pharmaceutical 35 diluents, excipients or carriers (collectively referred to herein as - 26- WO 98/57632 PCT/US98/12573 "carrier" materials) suitably selected with respect to the intended form of administration, that is, oral tablets, capsules, elixirs, syrups and the like, and consistent with conventional pharmaceutical practices. 5 For instance, for oral administration in the form of a tablet or capsule, the active drug component can be combined with an oral, non-toxic pharmaceutically acceptable inert carrier such as ethanol, glycerol, water and the like. Moreover, when desired or necessary, suitable binders, lubricants, disintegrating agents and 10 coloring agents can also be incorporated into the mixture. Suitable binders include, without limitation, starch, gelatin, natural sugars such as glucose or beta-lactose, corn sweeteners, natural and synthetic gums such as acacia, tragacanth or sodium alginate, carboxymethylcellulose, polyethylene glycol, waxes and the like. 15 Lubricants used in these dosage forms include, without limitation, sodium oleate, sodium stearate, magnesium stearate, sodium benzoate, sodium acetate, sodium chloride and the like. Disintegrators include, without limitation, starch, methyl cellulose, agar, bentonite, xanthan gum and the like. 20 The liquid forms in suitably flavored suspending or dispersing agents such as the synthetic and natural gums, for example, tragacanth, acacia, methyl-cellulose and the like. Other dispersing agents which may be employed include glycerin and the like. For parenteral administration, sterile suspensions and solutions are 25 desired. Isotonic preparations which generally contain suitable preservatives are employed when intravenous administration is desired. The compounds of the present invention can also be administered in the form of liposome delivery systems, such as small unilamellar vesicles, large unilamellar vesicles and multilamellar 30 vesicles. Liposomes can be formed from a variety of phospholipids, such as cholesterol, stearylamine or phosphatidylcholines. Compounds of the present invention may also be delivered by the use of monoclonal antibodies as individual carriers to which the compound molecules are coupled. The compounds of the 35 present invention may also be coupled with soluble polymers as - 27- WO 98/57632 PCT/US98/12573 targetable drug carriers. Such polymers can include polyvinyl pyrrolidone, pyran copolymer, polyhydroxypropylmethacryl amidephenol, polyhydroxy-ethylaspartamidephenol, or polyethyl eneoxidepolylysine substituted with palmitoyl residues. 5 Furthermore, the compounds of the present invention may be coupled to a class of biodegradable polymers useful in achieving controlled release of a drug, for example, polylactic acid, polyepsilon caprolactone, polyhydroxy butyric acid, polyorthoesters, polyacetals, polydihydro-pyrans, polycyanoacrylates and cross-linked or 10 amphipathic block copolymers of hydrogels. Compounds of this invention may be administered in any of the foregoing compositions and according to dosage regimens established in the art whenever specific blockade of the human alpha la adrenergic receptor is required. 15 The daily dosage of the products may be varied over a wide range from 0.01 to 1,000 mg per adult human per day. For oral administration, the compositions are preferably provided in the form of tablets containing 0.01, 0.05, 0.1, 0.5, 1.0, 2.5, 5.0, 10.0, 15.0, 25.0, 50.0 and 100 milligrams of the active ingredient for the symptomatic adjustment 20 of the dosage to the patient to be treated. A medicament typically contains from about 0.01 mg to about 500 mg of the active ingredient, preferably, from about 1 mg to about 100 mg of active ingredient. An effective amount of the drug is ordinarily supplied at a dosage level of from about 0.0002 mg/kg to about 20 mg/kg of body weight per day. 25 Preferably, the range is from about 0.001 to 10 mg/kg of body weight per day, and especially from about 0.001 mg/kg to 7 mg/kg of body weight per day. The compounds may be administered on a regimen of 1 to 4 times per day. Compounds of this patent disclosure may be used alone at 30 appropriate dosages defined by routine testing in order to obtain optimal antagonism of the human alpha la adrenergic receptor while minimizing any potential toxicity. In addition, co-administration or sequential administration of other agents which alleviate the effects of BPH is desirable. Thus, in one embodiment, this includes 35 administration of compounds of this invention and a human - 28- WO 98/57632 PCT/US98/12573 testosterone 5c-reductase inhibitor. Included with this embodiment are inhibitors of 5-alpha reductase isoenzyme 2. Many such compounds are now well known in the art and include such compounds as PROSCAR®, (also known as finasteride, a 4-Aza-steroid; see US Patents 4,377,584 and 5 4,760,071, for example). In addition to PROSCAR®, which is principally active in prostatic tissue due to its selectivity for human 5a-reductase isozyme 2, combinations of compounds which are specifically active in inhibiting testosterone 5-alpha reductase isozyme 1 and compounds which act as dual inhibitors of both isozymes 1 and 2, are useful in 10 combination with compounds of this invention. Compounds that are active as 5ac-reductase inhibitors have been described in WO93/23420, EP 0572166; WO 93/23050; WO93/23038,; WO93/23048; WO93/23041; WO93/23040; WO93/23039; W093/23376; W093/23419, EP 0572165; W093/23051. 15 The dosages of the alpha la adrenergic receptor and testosterone 5-alpha reductase inhibitors are adjusted when combined to achieve desired effects. As those skilled in the art will appreciate, dosages of the 5-alpha reductase inhibitor and the alpha la adrenergic receptor antagonist may be independently optimized 20 and combined to achieve a synergistic result wherein the pathology is reduced more than it would be if either agent were used alone. In accordance with the method of the present invention, the individual components of the combination can be administered separately at different times during the course of therapy or concurrently in 25 divided or single combination forms. The instant invention is therefore to be understood as embracing all such regimes of simultaneous or alternating treatment and the term "administering" is to be interpreted accordingly. Thus, in one preferred embodiment of the present 30 invention, a method of treating BPH is provided which comprises administering to a subject in need of treatment any of the compounds of the present invention in combination with finasteride effective to treat BPH. The dosage of finasteride administered to the subject is about 0.01 mg per subject per day to about 50 mg per subject per day in - 29- WO 98/57632 PCT/US98/12573 combination with an alpha la antagonist. Preferably, the dosage of finasteride in the combination is about 0.2 mg per subject per day to about 10 mg per subject per day, more preferably, about 1 to about 7 mg per subject to day, most preferably, about 5 mg per subject per 5 day. For the treatment of benign prostatic hyperplasia, compounds of this invention exhibiting alpha la adrenergic receptor blockade can be combined with a therapeutically effective amount of a 5a-reductase 2 inhibitor, such as finasteride, in addition to a 5X 10 reductase 1 inhibitor, such as 4,73-dimethyl-4-aza-5a-cholestan-3 one, in a single oral, systemic, or parenteral pharmaceutical dosage formulation. Alternatively, a combined therapy can be employed wherein the alpha la adrenergic receptor antagonist and the 5c reductase 1 or 2 inhibitor are administered in separate oral, 15 systemic, or parenteral dosage formulations. See, e.g., U.S. Patent No.'s 4,377,584 and 4,760,071 which describe dosages and formulations for 5a-reductase inhibitors. Abbreviations used in the instant specification, particularly the Schemes and Examples, are as follows: 20 AcOH or HOAc = acetic acid BCE = bromochloroethane Boc or BOC = t-butyloxycarbonyl Boc20 = di-tert-butyl dicarbonate 25 BOPC1 = bis(2-oxo-3-oxazolidinyl)phosphinic chloride Cbz-C1 = benzyloxycarbonyl chloride DEAD = diethylazodicarboxylate DMF = N,N-dimethylformamide DMSO = dimethylsulfoxide 30 D-S = Dean Stark EDCI = 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride Et = ethyl Et3N = triethylamine 35 EtOAc = ethyl acetate - 30- WO 98/57632 PCT/US98/12573 EtOH = ethanol FABLRMS = fast atom bombardment low resolution mass spectroscopy HPLC = high performance liquid chromatography 5 HOBt = 1-hydroxy benzotriazole hydrate i-PrOH = 2-propanol i-Pr2NEt = diisopropylethylamine LAH = lithium aluminum hydride mCPBA = meta-chloroperbenzoic acid 10 Me = methyl MeOH = methanol NMR = nuclear magnetic resonance PCTLC = preparative centrifugal thin layer chromatography 15 PEI = polyethylenimine Ph = phenyl RT = retention time tBuOH = tert-butanol TEBAC = benzyltriethylammonium chloride 20 TFA = trifluoroacetic acid THF = tetrahydrofuran TLC = thin layer chromatography TMS = trimethylsilyl Tos20 = p-toluenesulfonicanhydride 25 Triton B = N-benzyltrimethylammonium hydroxide The compounds of the present invention can be prepared readily according to the following reaction schemes and examples, or modifications thereof, using readily available starting materials, 30 reagents and conventional synthesis procedures. In these reactions, it is also possible to make use of variants which are themselves known to those of ordinary skill in this art, but are not mentioned in greater detail. Unless otherwise indicated, all variables are as defined above. The general synthetic approach to analogs claimed within 35 this application is outlined in Scheme 1. Starting from ketone A, -31- WO 98/57632 PCT/US98/12573 reductive amination with a mono blocked diamino species provides an intermediate which can be alkylated on the newly generated secondary amine. Deprotection of the terminal blocked amine and acylation, alkylation, etc. provides the desired analogs. 5 For example, a substituted benzyl nitrile, sulphone, etc. can be added to methyl acrylate, submitted to Dieckman cyclization, hydrolyzed and decarboxylated providing appropriately substituted ketones A. Scheme 2 depicts a more specific series of compounds derived from Michael addition of substituted benzyl nitriles to methyl 10 acrylate, Dieckman cyclization, providing the P-keto ester which can be either: (a) submitted to a reductive amination and carried on to final products, (b) enolized and alkylated then reductively aminated, deprotected and further manipulated providing further substituted analogs; or (c) hydrolyzed and decarboxylated and run through the 15 above described conditions producing the desired antagonists. Another strategy for the synthesis of some geminally disubstituted cyclic ketones, in particular, 4,4-disubstituted cyclohexanones was accomplished as outlined in Scheme 3 starting from benzophenone derivatives and substituted methyl vinyl ketones 20 which under basic conditions lead to the 4,4-diaryl cyclohex-2-en-l-ones in good yield. Subsequent hydrogenation, reductive amination and deprotection provided the appropriate acylation/alkylation precursors. Some examples were prepared by first assembling the appropriately substituted amino bearing cycloalkyl, then allylating the 25 amino moiety. This approach is described in Scheme 4. Starting with a cycloalkanone, for instance, 4-cyano 4-phenyl piperidone, reductive amination with ammonium acetate and sodium cyanoborohydride provides both the cis and trans 1-amino cyclohexanes. The ratio of these isomers is modulated by the choice of an appropriate reducing reagent, 30 The incipient amino group could be protected, alkylated, deprotected and alkylated again providing more funtionalized analogs. Antagonists with alkyl (straight or branched chain) can be assembled by reductive amination of the prerequisite aminoalcohol and a cycloketone, for example, 4-cyano 4-phenylcyclohexanone, Scheme 5. 35 Boc protection of the amine, followed by tosylation of the hydroxy and - 32- WO 98/57632 PCT/US98/12573 displacement by the lithium or sodium salt of the desired Q group completes the synthesis of the targeted antagonists. The selective acylation of the primary amines was accomplished by treatment with nearly equimolar quantities of the 5 activated termini species (i.e., the "Q" groups). The activated termini species comprising the "Q" groups are readily prepared by one of ordinary skill in the art. For example, oxazolidinones are prepared and activated in general by published and well developed chemistry, in particular, of Evans. [Evans, D.A.; Nelson, J.V.; Taber, T.R. Top. 10 Stereochem. 13, 1 (1982)] The starting materials, in general, are natural and unnatural amino acids. For instance, some of the preferred compounds are prepared from substituted phenyl glycine derivatives, which after reduction of the carboxylate and a phosgene equivalent mediated cyclization provides the substituted oxazolidinone ring system. 15 Deprotonation with n-butyl lithium and addition to a THF solution of p nitrophenylchloroformate produces the stable, isolable "activated"oxazolidinone (oxa). Hydantoins and cycloimide were prepared in two chemical steps from ketones as outlined in the literature. More specifically, 20 hydantoins were prepared according to known methodology, e.g., J.J. Edmunds et al., J. Med. Chem. 1995, 38, pp. 3759-3771; J.H. Poupart et al., J. Chem. Res. 1979, pp. 174-175. Saccharins were prepared according to known methods, e.g., page 40 and Examples 21 and 22 of PCT International Application Publication No. WO96/25934, published 25 August 29, 1996. The oxazolidinones were synthesized independently in racemic form, and then separated utilizing preparative chiral HPLC. Their optical rotations were recorded. Then they were activated and reacted with prerequisite amines. From the receptor binding studies, a 30 preferred isomer was identified, the (+) rotational isomer. The absolute configurations were determined to be (S) for the oxazolidinones by correlating their optical rotations with x-ray crystal structures obtained of fragments involved in the production of the antagonists. - 33- WO 98/57632 PCT/US98/12573 SCHEME 1: Synthesis of Cycloalkvlaminoethylamines In general:

R

3

R

6 0- 1 NHBOC R n H2 6 1R n R 2 X R1

R

3 R R 3 6 -1 NaCNBH 3 NHBOC O MeOH \" N m m H R 3

R

6 A R n R! o 0-1 R. HCI R 3

R

6 R3 R ' NH NHBOC EtOAc N NH 2 HRm R 2

R

3

R

6 m R 2 R3 R 6 Acids, activated carbamates etc 1R, , (R:.R 1 i. -1 Re o - 1 Acids, R! R3 R6 -1 R R activated

NH

2 N Q carbamrnates, m H R 3

R

6 m R 2 R3 R 6 etc

R

2 =H HI EtOAc R R n

R

3

R

6 mN NHBOC m H R 3

R

6 - 34- WO 98/57632 PCT/US98/12573 SCHEME 1 (Cont'd) For example,

'CO

2 Me R KOtBu 17 R -- 1/

CO

2 Me (R )s Triton-B (R17)s THF tBuOH

CO

2 Me R

C(

R (R AcOH / _

SCO

2 Me (R17)s< (R17)s Aq HCI 0O A - 35- WO 98/57632 PCT/US98/12573 SCHEME 2 CN CO 2 Me ON C O 2 M KOtBu CN (R'7)s- OM (R )ts Triton-BT tBuOH

CO

2 Me C N 1C7O 2 Me AcOH CN (R 17)s / 0 Aq HCI (R)s O A B I CN CO2Me H2N NH2 (R17)s l A ' NNH2 D-S trap, H reflux C 6

H

6 then conc'd in CN O 2 M e ,CO2Me vacuo; MeOH(R17 NaCNBH 3 s N H2 'N NH H - 36- WO 98/57632 PCT/US98/12573 SCHEME 2 (Cont'd) F CN 0 1C2Me CN F N R7 "')N < s N pNH2 O H 0~N

N

2 H O 0 F 3 H2 NH2 17 F D-S trap, (R17) s " reflux C 6

H

6 then conc'd in N N H 2 vacuo; MeOH, CN H NaCNBH 3 (R17)s 'H N p N2H 2 H (Ri7s 17

O

H NN F - 37-F - 37- WO 98/57632 PCT/US98/12573 SCHEME 3 II /Sq 0 0 0 H 2 /Pd-C Base H

SH

2 N0 NHBOC N NHP AcOH/MeOH SNaCNBH 3 P = BOC HCI P = H*HCI EtOAc H o F EDCI N N HO F HOBt 5 -38- WO 98/57632 PCT/US98/12573 SCHEME 3 (Cont'd) F H 0 2 H 0 0 2 N- 39 0 - 39- WO 98/57632 PCT/US98/12573 SCHEME 4 CN NH 4 OAc CN 1) BOC20/THF NaCNBH 3 2) NaH/R 2 X o MeH

NH

2 3) HCI/EtOAc

JNH

2 NB r(CH 2

)

2 -nsaccharin 2 iPr 2 NEt/DMF NCN !O~O N N% 0 0 - 40- WO 98/57632 PCT/US98/12573 SCHEME 5 AcOH or CN Ti(OiPr) 4 NaCNBH 3 MeOH O H2N, (CH 2

)

0 -20H CN N 1- (CH2)0-20H 1) Boc20 H 2) Tos20 CN C1-2 F N ( 2)0-2OTos F Boc 1) nBuLi, HN O 0 2) HCI-EtOAc F | CN N NO I ON 0 -41- WO 98/57632 PCT/US98/12573 SCHEME 5 (Cont'd) H-. y 1 1 N1 R14 0

R

13 1 H O N. R' R H Z

R

13 C R14 CN

R

8 .N." 1NaH ON 1 R 13 2) HCI-EtOAc NH RRI4 H H 0 S(X)q cN N0 1- 122 HN NiI H 0-2 N o7o - 42- WO 98/57632 PCT/US98/12573 The following examples are provided to further define the invention without, however, limiting the invention to the particulars of these examples. 5 EXAMPLE 1 CN Oi CN t h NH 2 II NH2 cis 1-amino 4-cyano 4-phenylcyclohexane and trans 1-amino 4-cyano 4 phenvylcvclohexane A solution of 4-cyano 4-phenylcyclohexanone (6.0 g, 30 10 mmol), 8.0 g 4A molecular sieves and ammonium acetate (23.2 g, 300 mmol) in methanol (200 mL) (1.5 h premixed) was treated with sodium cyanoborohydride (1.9 g, 30 mmol) at room temperature. The resulting mixture was stirred at room temperature (60 min), then concentrated in vacuo and the residue dissolved in EtOAc and sodium bicarbonate 15 solution. The aqueous layer was extracted with one additional portion of EtOAc, the combined organic extracts were washed with brine, dried over Na2SO4, and concentrated under reduced pressure. PCTLC (SiO 2 , 6 mm, 0 - 10% MeOH - CHC1 3 ) provided the two title compounds. The cis (more polar isomer) and trans isomer (less polar isomer). 20 cis isomer: 1 H NMR (CDC1 3 , 300 MHz) 7.49 (br d, 2 H, ArH), 7.39 (br t, 2 H, ArH), 7.33 (br m, 1 H, ArH), 2.76 (br m, 1 H, CHNH2), 2.21 (br d, 2 H), 2.03 (br dd, 2 H), 1.88 (br ddd, 2 H), 1.73 (br ddd, 2 H) Anal. Calcd for C 1 3

H

1 6

N

2 : C = 77.96, H = 8.05, N = 13.99. Found: C = 78.00, H = 7.94, N = 13.82. 25 HPLC (Vydac; C18; diameter = 4.6 mm; length = 150 mm; gradient = H 2 0 [0.1% H 3

PO

4 ] - CH 3 CN, 95% - 5%, 5% - 95%, over 16 minutes, 2 ml/min flow rate) RT = 5.87 min; focus = 215 nm; 97.4% pure. trans isomer: 1 H NMR (CDC1 3 , 300 MHz) 7.53 (br m, 2 H, ArH), 7.38 (br m, 2 H, ArH), 7.34 (br m, 1 H, ArH), 3.37 (dd, 1 H, CHNH2), 2.36 (ddd, 30 2 H), 2.08 (br ddd, 2 H), 1.91 (br dd, 2 H), 1.67 (br dd, 2 H) -43- WO 98/57632 PCT/US98/12573 Anal. Caled for C 1 3

H

1 6

N

2 * 0.15 H20 C = 77.96, H = 8.05, N = 13.99. Found: C = 76.85, H = 7.87, N = 13.82. HPLC (Vydac; C18; diameter = 4.6 mm; length = 150 mm; gradient = H 2 0 [0.1% H 3

PO

4 ] - CH 3 CN, 95% - 5%, 5% - 95%, over 16 5 minutes, 2 ml/min flow rate) RT = 5.68 min; focus = 215 nm; 98.4% pure. EXAMPLE 2 CN 'cII NHBOC cis 1-N-(1.,1-dimethylethoxycarbonyl)amino-4-cvano 4-phenvylcyclohexane 10 A solution of the amine (3.0 g, 15 mmol) and BOC20 (3.3 g, 15 mmol) in THF (40 mL) was mixed at room temperature for lh. The resulting mixture was concentrated in vacuo to afford the title compound. cis isomer: 1 H NMR (CDC1 3 , 300 MHz) 7.47 (br m, 2 H, ArH), 7.40 (br m, 15 2 H, ArH), 7.35 (br m, 1 H, ArH), 4.52 (br d, 1 H, NHC=O), 3.52 (br s, 1 H, CHNH), 2.40 (br m, 4 H), 1.92 (br ddd, 2 H), 1.71 (br ddd, 2 H), 1.46 (br s, 9 H) EXAMPLE 3 CN "'NBOC 20 CH, cis 1-N-[(1,1-dimethylethoxycarbonyl)methyl]amino-4-cyano 4 pheny1cvclohexane A solution of the BOC carbamate (2.0 g, 15 mmol) in DMF (10 mL) was treated with NaH (290 mg, 7.33 mmol) and iodomethane at -44- WO 98/57632 PCT/US98/12573 0OC for 4h. The resulting mixture was diluted with water (50 mL), extracted with EtOAc (3 x 50 mL), washed with brine (1 x 75 mL), dried (Na2SO4), filtered and concentrated in vacuo. PCTLC 0 - 50 % EtOAc/hexane afforded the title compound. 5 cis isomer: 1H NMR (CDC1 3 , 300 MHz) 7.48 (br m, 2 H, ArH), 7.43 (br m, 2 H, ArH), 7.34 (br m, 1 H, ArH), 4.16 (br d, 1 H, CHNH), 2.83 (br s, 3 H, NCH3), 2.25 (br d, 2 H), 1.80 - 2.10 (br m, 6 H), 1.49 (br s, 9 H) EXAMPLE 4 01CN 10

NHCH

3 cis 1-N-methylamino-4-cyano 4-phenvycyclohexane A solution of the BOC carbamate (1.5 g, 4.8 mmol) was treated saturated HC1-EtOAc at 0oC. The resulting mixture was allowed to warm to room temperature (lh) then concentrated in vacuo affording 15 the title compound. Anal. Calcd for C 1 4

H

1 8

N

2 * HCI: C = 67.05, H = 7.64, N = 11.17. Found: C = 66.92, H = 7.51, N = 11.20. HPLC (Vydac; C18; diameter = 4.6 mm; length = 150 mm; gradient = H 2 0 [0.1% H 3

PO

4 ] - CH 3 CN, 95% - 5%, 5% - 95%, over 16 20 minutes, 2 ml/min flow rate) RT = 5.91 min; focus = 215 nm; 100% pure. - 45- WO 98/57632 PCT/US98/12573 EXAMPLE 5 G CN I H

CH

3 (4-cyano-4-phenyl-cyclohexyl)-[3-(2,2-di-p-tolyl-acetylamino) propyl]-methyl-ammonium chloride 5 A solution of the amine (75.0 mg, 0.35 mmol), Br(CH2)3NHCOCH(p-tolyl)2 (140 mg, 0.385 mmol) and iPr2NEt (54.3 mg, 0.42 mmol) was stirred in DMF (1 mL) at room temperature (12h). The solvent was removed in vacuo and submitted to PCTLC (SiO 2 , 2mm, CHC1 3 - 90:10:1 CHC1 3

:CH

3 OH:NH4OH providing the title compound 10 which was converted to the hydrochloride salt by treatment with HC1 EtOAc. cis isomer: 1 H NMR (DMSO-d6, 400 MHz) 8.44 (br t, 1 H, NH), 7.53 (br d, 2 H, ArH), 7.47 (br t, 2 H, ArH), 7.39 (br m, 1 H, ArH), 7.18 (br dd, 4 H, ArH), 7.1 (br d, 4 H, ArH), 4.83 (br s, 1 H, CHC=O), 3.19 (br m, 2 H), 3.04 15 (br m, 2 H), 2.50 (br s, 7 H, CHNCH3 and ArCH3), 2.24 (br s, 3 H, NCH3), 2.21 (br m, 2 H), 2.10 (br d, 2 H), 1.95 (br m, 2 H), 1.82 (br m, 2 H), 1.72 (br m, 2H) Anal. Calcd for C 3 3

H

3 9

N

3 0 * 2H20: C = 70.00, H = 7.83, N = 7.42. Found: C = 69.92, H = 7.51, N = 7.08. 20 HPLC (Vydac; C18; diameter = 4.6 mm; length = 150 mm; gradient = H 2 0 [0.1% H 3

PO

4 ] - CH 3 CN, 95% - 5%, 5% - 95%, over 16 minutes, 2 ml/min flow rate) RT = 10.66 min; focus = 215 nm; 96.7% pure. - 46- WO 98/57632 PCT/US98/12573 EXAMPLE 6 0c CN H,, 0 (4-cyano-4-phenyl-cyclohexyl)-methyl-[4-(1,1,3-trioxo-1,3-dihydro 116-benzo[dlisothiazol-2-v1l)-butvyl]-ammonium chloride 5 A solution of the amine (99.6 mg, 0.465 mmol), Br(CH2)4 N(saccharin) (178 mg, 0.558 mmol) and iPr2NEt (72 mg, 0.558 mmol) was stirred in DMF (1 mL) at room temperature (12h). The solvent was removed in vacuo and submitted to PCTLC (SiO 2 , 2mm, CHC1 3 - 95:5 CHC1 3

:CH

3 OH providing the title compound (193.2 mg, 210 mg 10 theoretical, 92%) which was converted to the hydrochloride salt by treatment with HC1-EtOAc. cis isomer: 1 H NMR (CDC13, 300 MHz) 8.07 (dd, 1 H, ArH), 7.90 (br dd, 1 H, ArH), 7.87 (br m, 2 H, ArH), 7.48 (br d, 2 H, ArH), 7.28 - 7.42 (br m, 3 H, ArH), 3.83 (t, 2 H, J = 7.5 Hz), 2.56 (br m, 3 H), 2.32 (br 2, 3 H, NCH3), 15 2.24 (br d, 2 H), 1.80 - 2.00 (br m, 8 H), 1.60 (br m, 2 H). Anal. Calcd for C 2 5

H

2 9

N

3 0 3 S * 1 HC1 & 0.25 H20: C = 60.96, H = 6.24, N = 8.53. Found: C = 60.97, H = 6.08, N = 8.57. HPLC (Vydac; C18; diameter = 4.6 mm; length = 150 mm; gradient = H 2 0 [0.1% H 3

PO

4 ] - CH 3 CN, 95% - 5%, 5% - 95%, over 16 20 minutes, 2 ml/min flow rate) RT = 8.53 min; focus = 215 nm; 100% pure. - 47- WO 98/57632 PCT/US98/12573 EXAMPLE 7 01CN O

CH

3 00 (4-cyano-4-phenyl-cyclohexyl)-methyl-[3-(1,1,3-trioxo-1,3-dihydro 116-benzo[disothiazol-2-vl)-propyl] -ammonium chloride 5 A solution of the amine (80.0 mg, 0.3733 mmol), Br(CH2)3 N(saccharin) (119.2 mg, 0.392 mmol) and iPr2NEt (53 mg, 0.411 mmol) was stirred in DMF (1 mL) at room temperature (12h). The solvent was removed in vacuo and submitted to PCTLC (SiO 2 , 2mm, CHC1 3 - 95:5 CHC1 3

:CH

3 OH providing the title compound. 10 cis isomer: 1 H NMR (CDC13, 300 MHz) 8.04 (dd, 1 H, ArH), 7.90 (br dd, 1 H, ArH), 7.87 (br m, 2 H, ArH), 7.49 (br d, 2 H, ArH), 7.28 - 7.42 (br m, 3 H, ArH), 3.83 (t, 2 H, J = 7.5 Hz), 2.64 (br t, 2 H), 2.56 (br m, 1 H), 2.32 (br 2, 3 H, NCH3), 2.24 (br d, 2 H), 1.80 - 2.00 (br m, 8 H). Anal. Calcd for C 2 4

H

2 7

N

3 0 3 S * 0.25 CHC13: C = 65.12, H = 15 6.15, N = 9.47. Found: C = 65.19, H = 5.76, N = 9.34. EXAMPLE 8 CN CN CO 2 Me

CO

2 Me

CO

2 Me A B 20 A: 5-nitrilo-4-o-tolyl-pentanoic acid methyl ester B: 4-cyano-4-o-tolyl-heptanedioic acid dimethyl ester A solution of 2-methylbenzyl nitrile (25.0 g), methyl acrylate (75 mL) and Triton-B (40 mL) in t-butanol (90 mL) was refluxed (12 h). The solvent was removed in vacuo and submitted to SGC (SiO 2 , 10 cm x - 48- WO 98/57632 PCT/US98/12573 30 cm, 0 - 15% EtOAc - hexane) affording the mono addition product and the desired bis addition compound (5). A: 1 H NMR (CDC1 3 , 300 MHz) 7.42 (m, 1 H, ArH), 7.20 (m, 3 H, ArH), 4.34 (dd, 1 H, CHCN), 3.69 (s, 3 H, OMe), 2.57 (m, 2 H), 2.37 (s, 3 H, 5 Me), 2.16 (m, 2 H). B: 1 H NMR (CDC1 3 , 300 MHz) 7.42 (m, 1 H, ArH), 7.20 (m, 3 H, ArH), 3.62 (s, 6 H, OMe), 2.57 (m, 4 H), 2.54 (s, 3 H, Me), 2.31 (m, 2 H). EXAMPLE 9 I CN \ N CO 2 Me 10 0 5-cvano-2-oxo-5-o-tolyl-cyclohexanecarboxylic acid methyl ester A solution of the diester (9.38 g, 29.4 mmol) in THF (200 mL) was treated with KOt-Bu (6.6 g, 58.74 mmol) at 0oC then heated to reflux (20 min). The solvent was removed in vacuo and submitted to SGC (SiO 2 , 15 6 cm x 20 cm, 15% EtOAc - hexane) affording desired product and some decarboxylated material. 1 H NMR (CDC1 3 , 300 MHz) consistent with assigned structure. FABLRMS m/e 272.22 g/mole (M++H, C 1 6

H

1 7 N0 3 = 272 20 g/mole.) EXAMPLE 10 CN 4-cyano(2-methylphenv1)-cyclohexan- 1-one - 49- WO 98/57632 PCT/US98/12573 A solution of the ketoester (5.0 g, 18.4 mmol) in AcOH (100 mL) was treated with 10% aqueous H2SO4 (10 mL) at 0 0 C then heated to reflux (24 h). The solvent was removed in vacuo, diluted with EtOAc (100 mL) and water (100 mL), partitioned, washed with brine (75 mL), dried 5 (Na2SO4), filtered and concentrated in vacuo and submitted to SGC (SiO 2 , 5 cm x 20 cm, 0 - 15% EtOAc - hexane) affording the ketone. 1 H NMR (CDC1 3 , 300 MHz) 7.24 (m, 4H, ArH), 2.95 (ddd, 1 H, CHCN), 2.70 (s, 3 H, Me), 2.60 (m, 4 H), 2.20 (ddd, 2 H). 10 EXAMPLE 11 ICN ICN O CO 2 Me

CO

2 Me N NHBOC N-sp NHBOC H H 2-(2-tert-butoxycarbonylamino-ethylamino)-5-cyano-5-o-tolyl cyclohexanecarboxylic acid methyl ester A solution of the ketoester (0.8 g, 3.75 mmol), amine (0.601 g, 15 3.75) and acetic acid (0.236 g, 18.75 mmol) in MeOH (10 mL) was treated with NaBH3CN (0.236 g, 3.75 mmol) at room temperature (12 h). The solvent was removed in vacuo , diluted with DCM (25 mL) and saturated aqueous sodium bicarbonate (25 mL), partitioned, extracted with DCM (2 x 25 mL), washed with saturated aqueous sodium bicarbonate (2 x 25 20 mL) and brine (50 mL), dried (Na2SO4), filtered and concentrated in vacuo and submitted to PCTLC (SiO 2 , 4 mm, 90/10/1 CHC13-MeOH NH4OH) the titled trans (A) and cis (B) amines. B 1H NMR (CDC1 3 , 400 MHz) 7.24 (m, 4 H, ArH), 4.98 (br s, 1 H, NHBOC), 3.75 (s, 3 H, OMe), 3.18 (br d, 2 H, 2.89 (br ddd, 2 H), 2.80 25 (ddd,1 H), 2.68 (m, 1 H), 2.64 (s, 3 H, Me), 2.54 (ddd, 1 H), 2.45 (ddd, 1 H), 2.29 (ddd, 1 H), 2.01 (dd, 1 H), 1.84 (ddd, 1 H), 1.75 (ddd, 1 H), 1.45 (s, 9 H, C(Me)3) - 50- WO 98/57632 PCT/US98/12573 EXAMPLE 12 ICN ICN 'N- NH2 N -p NH2 H H A B 5 [2-(4-cyano-4-o-tolyl-cyclohexylamino)-ethyl]-carbamic acid tert-butyl ester A solution of the ketone (0.6 g, 2.813 mmol), ethylene diamine (0.845 g, 14.1 mmol) and p-toluene sulphonic acid (0.026 g, 0.141 mmol) in benzene (10 mL) was refluxed under a Dean-Stark trap until 10 cessation of water azeotrope. The solvent was removed in vacuo , diluted with MeOH (25 mL) and treated with NaBH3CN (0.159 g, 2.55 mmol) at room temperature (1 h). The solvent was removed in vacuo , diluted with DCM 25 mL) and saturated aqueous sodium bicarbonate (25 mL), partitioned, extracted with DCM (2 x 25 mL), washed with saturated 15 aqueous sodium bicarbonate (2 x 25 mL) and brine (50 mL), dried (Na2SO4), filtered and concentrated in vacuo and submitted to PCTLC (SiO 2 , 2 cm, 80/20/2 CHC13-MeOH-NH4OH) the titled trans (minor) (A) and cis (major) (B) amines. A trans: 1 H NMR (CDC1 3 , 300 MHz) 7.38 (m, 1 H), 7.20 (m, 3 H, ArH), 20 2.98 (br m, 1 H, CHNH), 2.80 (br t, 2 H), 2.65 (s, 3 H, Me), 2.64 (br m, 2 H), 2.38 (br dd, 2 H) 2.09 (br m, 4 H), 1.83 (br d, 2 H). B cis: 1 H NMR (CDC1 3 , 300 MHz) 7.24 (m, 4 H, ArH), 2.83 (br dd, 2 H), 2.75 (br dd, 2 H), 2.65 (s, 3 H, Me), 2.55 (br m, 1 H, CHNH), 2.43 (br m, 2 H), 2.16 (br d, 2 H), 1.67 (br d, 4 H). 25 -51- WO 98/57632 PCT/US98/12573 EXAMPLE 13 F F F NCN H 0 0 (+)-2-Oxo-4-(3,4,5-trifluorophenyl)-oxazolidine-3-carboxylic acid [2-(4-cyano-4-phenvyl-cclohexylamino)-ethyl1] amide 5 To a solution of 1-[(2-amino-ethyl)-amino]-4-cyano-4-phenyl hexane (25 mg, 0.103 mmol) in 10 mL of THF, 4-(3,4,5-trifluorophenyl)-2 oxo-oxazolidine-3-carboxylic acid-4-nitro-phenyl ester (30 mg, 0.079 mmol) was added and the resulting yellow solution was stirred under argon atmosphere for 2 h at room temperature. The solvent was 10 removed in vacuo and the residue was purified by column chromatography over silica gel with 1:1 hexane/EtOAc followed by MeOH:EtOAc=1:9 (Rf= 0.60, MeOH:EtOAc=1:3 ) to obtain the title compound. The compound was dissolved in CH 2 C1 2 (3 mL) and was treated with 1N HC1 in ether (1 mL). The solvent was removed in vacuo 15 to give the corresponding hydrochloride salt as a pale yellow solid. M. P. 130-134 0 C; [o]D = +43.5, (c = 0.25, MeOH); Anal. Calcd. For

C

2 5

H

2 6

N

4 0 3

F

3 C1 1.10 C 3

H

6 0: C, 57.92; H, 5.60; N, 8.55. Found: C, 58.33; H, 5.90; N, 8.52. - 52- WO 98/57632 PCT/US98/12573 EXAMPLES 14 AND 15 F F

CO

2

CH

3 H N N H YlIi 0 O0 (+)-2-Oxo-4-(3,4-difluorophenyl)-oxazolidine-3-carboxylic acid [2-(4-phenyl-4-methoxycarbonyl-cyclohexylamino)-ethyl] amide 5 (cis isomer) and (+)-2-Oxo-4-(3,4-difluorophenyl)-oxazolidine-3-carboxylic acid [2-(4-phenyl-4-methoxycarbonyl-cyclohexylamino)-ethyl] amide (trans isomer) a. 2-[4-Cyano-4-phenylcyclohex-1-yl] aminoethylamine. 10 A mixture of 4-cyano-4-phenylcyclohexanone (48.7 mmol) and ethylenediamine (8.78 g, 146 mmol) and p-toluenesulfonic acid (92 mg) in benzene (200 mL) was refluxed for 4 h in Dean-Stark trap to remove the water that formed. Solvent was evaporated and the residue was redissolved in methanol (60 mL) and cooled to 0 C. To this, sodium 15 borohydride (6.4 5 g) was added in portions and the mixture was stirred at room temperature for 3 h. Solvent was evaporated, the residue was dissolved in dichloromethane (300 mL), washed with brine (3 X 500 mL), dried (potassium carbonate), and the solvent evaporated to leave the product as a pale yellow viscous oil (90-95%). The 1 H-NMR showed the 20 product to be pure and found to contain the cis/trans isomers in the ratio of about 9:1. A careful chromatography of this mixture with chloroform/methanol/2M ammonia in methanol (100/10/5 to 100/20/10) gave some earlier fractions enriched in the trans isomer relative to the amino and cyano groups. The fractions eluted at the end were almost 25 pure cis isomer relative to the amino and cyano groups. b. 2-[4-Methoxycarbonyl-4-phenylcyclohex-1-yl]aminoethylamine. - 53- WO 98/57632 PCT/US98/12573 A mixture of 2-[4-cyano-4-phenylcyclohex-1 yl]aminoethylamine (2.34 g, 10 mmol) and concentrated sulfuric acid (20 mL) was heated at 80-85' C for 10 h. It was cooled to room temperature, mixed with anhydrous methanol (200 mL), and refluxed for 20 h. 5 Solvent was evaporated and the residue was poured onto ice (200 g) and basified to pH 11 by addition 6N NaOH. It was extracted with dichloromethane (4 X 125 mL), dried (potassium carbonate) and solvent evaporated to leave the product as an oil (2.1 g, 76%). 1 H-NMR showed this product to be pure and a mixture of cis and trans isomers. It was 10 used in the next step without any further purification. c. (+)-2-Oxo-4-(3,4-difluorophenyl)-oxazolidine-3-carboxylic acid [2 (4-phenyl-4-methoxycarbonyl-cyclohexylamino)-ethyl] amide (cis isomer) and 15 (+)-2-Oxo-4-(3,4-difluorophenyl)-oxazolidine-3-carboxylic acid [2 (4-phenyl-4-methoxycarbonyl-cyclohexylamino)-ethyl] amide (trans isomer). A mixture of 4-(3,4-difluorophenyl)-3-(4-nitrophenyloxy carbonyl)-2-oxo-oxazolidine (50 mg, 0.123 mmol) and 2-[4-methoxy 20 carbonyl-4-phenylcyclohex-1-yl]aminoethylamine (75 mg) in dichloromethane (6 mL) was stirred at room temperature and the product formed was purified by preparative TLC on silica gel using ethyl acetate as the eluent. There were two bands for the two isomers, the higher band was the minor product ( 1 H-NMR confirmed it to be the cis 25 isomer with respect to methoxycarbonyl and amine groups) and the lower band was the major product ( 1 H-NMR confirmed it to be the trans isomer with respect to methoxycarbonyl and amine groups). The HC1 salt was made by treatment with 1N HC1 in ether. 30 (+)-2-Oxo-4-(3,4-difluorophenyl)-oxazolidine-3-carboxylic acid [2 (4-phenyl-4-methoxycarbonyl-cyclohexylamino)-ethyl] amide (cis isomer). [a]D = 65.8 (c = 0.50 g, methanol); m.p. 146-148 C; Anal. Calcd. For: C 2 6

H

2 9

F

2

N

3 0 5 .HCl: C, 58.05; H, 5.62; N, 7.81. Found: C, 58.45; H, 5.51; N, 7.89. - 54- WO 98/57632 PCT/US98/12573 (+)-2-Oxo-4-(3,4-difluorophenyl)-oxazolidine-3-carboxylic acid [2 (4-phenyl-4-methoxycarbonyl-cyclohexylamino)-ethyl] amide (trans isomer). [ct]D = +66 (c = 0.48 g, methanol); m.p. 140-142 C; Anal. Calcd. For: C 2 6

H

2 9

F

2

N

3 0 5 .HC1.0.4H 2 0: 5 C, 57.16; H, 5.39; N, 7.61. Found: C, 57.28; H, 5.69; N, 7.79. EXAMPLE 16 F F

OCH

2

CH

3 CN H NN H Yii O 0 (+)-cis-2-Oxo-4-(3,4-difluorophenyl)-oxazolidine-3-carboxylic acid 10 [2-(4-evano-4-(2-ethoxyphenvl)-cyclohexylamino)-ethyl] amide a. 4-Cyano-4-(2-ethoxy)phenyl-2-methoxycarbonylcyclohexanone: A solution of 40% methanolic triton-B (1.8 mL) in t-butyl alcohol (3.7 mL) was slowly added to a solution of 2-ethoxybenzyl cyanide (3.0 g, 18.6 mmol) and methyl acrylate (5.4 mL, 60.0 mmol) in refluxing 15 t-butyl alcohol (5.4 mL). The mixture, after having been heated at reflux overnight, was concentrated. The residue was dissolved in chloroform (50 mL) and washed with 2N HC1 (40 mL) and water (40 mL), dried (Na 2

SO

4 ), filtered and concentrated to give a colorless oil (5.98 g, 96%). This oil (5.98 g, 17.9 mmol) was dissolved in dry toluene (50 mL), cooled 20 by an ice water bath and treated with NaH (60% oil dispersion, 804 mg, 20.1 mmol). The mixture was heated at reflux for 4 h. A solution of 2N acetic acid (21 mL) was added. The organic layer was separated, washed with NaHCO 3 solution, dried (Na 2

SO

4 ), filtered and concentrated to give a light brown liquid (4.76 g). It was dissolved in 25 CH 2 C1 2 and flash chromatographed over silica gel (320 g) eluting with EtOAc/hexane (1:10) to afford a white solid (2.09 g, 39%): mp 82-88 C; ESMS m/e = 302 (MH+). - 55- WO 98/57632 PCT/US98/12573 b. 4-Cyano-4-(2-ethoxy)phenylcyclohexanone: 4-Cyano-4-(2-ethoxy)phenyl-2-methoxycarbonyl cyclohexanone (1.06 g, 3.5 mmol) was heated at reflux in acetic acid (24 5 mL) and 10% H 2

SO

4 (13 mL) for 6 h. Extraction with benzene (3 x 10 mL), which was washed with K 2

CO

3 solution, dried (Na 2

SO

4 ), filtered and concentrated, gave a white solid (0.755g, 88%): mp 116-121 C. c. cis-4-Cyano-4-(2-ethoxy)phenylcyclohexyl-aminoethylamine: 10 4-Cyano-4-(2-ethoxy)phenylcyclohexanone (300 mg, 1.23 mmol) was mixed with ethylenediamine (420 mL, 6.28 mmol) and a catalytic amount of tosic acid monohydrate in benzene (10 mL) and heated at reflux for 6 h. The solvent was evaporated off and the residue dissolved in dry EtOH (10 mL). After treatment with NaBH 4 (47 mg, 1.24 15 mmol), the mixture was stirred at room temperature for 3 h. The solvent was evaporated off and the residue triturated with CH 2 C12, treated with anhydrous Na 2

SO

4 and filtered to afford a pale yellow oil (320 mg, 90%). 20 d. (+)-cis-2-Oxo-4-(3,4-difluorophenyl)-oxazolidine-3-carboxylic acid [2-(4-cyano-4-(2-ethoxyphenyl)-cyclohexylamino)-ethyl] amide: To cis-4-cyano-4-(2-ethoxy)phenylcyclohexylamino ethylamine (43 mg, 0.15 mmol) in dry THF (5 mL) was added (+)-4-(3,4 difluoro)phenyl-3-(4-nitro)phenoxycarbonyl-2-oxazolidone (50 mg, 0.14 25 mmol). The yellow solution was stirred at room temperature for 5 h before it was concentrated. The residue was dissolved in CHC1 3 and flash chromatographed over silica gel (18 g) eluting with EtOAc/hexane (1:1) and then EtOAc/2M NH 3 in MeOH (20:1) to give a colorless oil (41 mg, 58%). It was dissolved in CHC1 3

/CH

2 C1 2 and treated with 1M HC1 in 30 ether (120 ptL) to afford a white solid: mp 125 C (dec.); [c]D = 71.4 (2.1 mg/mL MeOH); ESMS m/e = 513 (MH+). Anal. Calcd. for

C

2 7

H

3 0

F

2

N

4 04"HCl0.5CHC1 3 : C, 54.26; H, 5.22; N, 9.20. Found: C, 53.98; H, 5.04; N, 8.89. - 56- WO 98/57632 PCT/US98/12573 EXAMPLE 17 CN O 0 F F [4-cyano-4-(2-methoxy-phenyl)-cyclohexyl]-(2-{[4-(3,4-difluoro-phenyl) -2 oxo-oxazolidine-3-carbonyll-amino}-ethyl)-ammonium chloride 5 To a solution of dry tetrahydrofuran (2 mL) containing 120 mg (0.33 mmole) of (+)-4-(3,4-difluorophenyl)-2-oxo-oxazolidine-3 carboxylic acid 4-nitrophenyl ester was added 87 mg (0.32 mmole) of 2 {[4-cyano-4-(2-methoxy)phenyl]cyclohexylamino}ethyl amine at ambient temperature under argon. The reaction mixture was stirred for 15 10 minutes and then was treated with 5 mL of 10% potassium carbonate solution. The reaction mixture was extracted with ethyl acetate (2X). The combined organic extracts were washed with brine, dried (sodium sulfate) and concentrated to give the crude product as an oil. Column chromatography of the reaction product on silica gel (methanol/ 15 methylene chloride gradient elution (1 to 4%)) afforded the title compound which was converted to its salt form with HCI in dioxane: m.p. 1400 C (d); HPLC = >99% pure at 215 nm NMR(CDC13, 400 MHz): Consistent with structure 20 assignment and confirms presence of solvent. FAB MS: 499 (M + + 1). Analysis for C26H28F2N404*HC1*0.3H20: Calculated: C, 57.79; H, 5.52; N, 10.37. Found: C, 57.76; H, 5.80; N, 10.47. 25 - 57- WO 98/57632 PCT/US98/12573 EXAMPLE 18 NCN 0 0 0 H N F N N 0 F F [4-cyano-4-(2-fluoro-phenyl)-cyclohexyl]-(2-{[4-(3,4-difluoro-phenyl) 2-oxo-oxazolidine-3-carbonyll-amino}-ethyl)-ammonium chloride 5 Using reaction conditions identical to those described in Example 19,139 mg (0.38 mmole) of (+)-4-(3,4-difluorophenyl)-2-oxo oxazolidine-3-carboxylic acid 4-nitrophenyl ester in 2 mL of tetrahydrofuran was converted to the title compound by reacting it with 100 mg (0.38 mmole) of 2-{[4-cyano-4-(2-fluoro)phenyl] 10 cyclohexylamino}ethyl. The chromatographed product was converted to the HC1 salt and lyophilized: HPLC = >99% pure at 215 nm NMR(CDC13, 400 MHz): Consistent with structure assignment and confirms presence of solvent. 15 FAB MS: 487 (M + + 1). Analysis for C25H25F3N402*HC1 *0.6H20: Calculated: C, 56.25; H, 5.14; N, 10.50. Found: C, 56.21; H, 4.74; N, 10.27. - 58- WO 98/57632 PCT/US98/12573 EXAMPLE 19

OCF

3 H O0 HH N N N ON H H II CN O F F 4-(3,4-difluoro-phenyl)-2-oxo-oxazolidine-3-carboxylic acid {2-[4 cvano-4-(2-trifluoromethoxv-phenv1)-cvclohexylamino]l-ethyll-amide 5 To a solution of dry N,N-dimethylformamide (5 mL) containing 137 mg (0.34 mmole) of 2-{[4-cyano-4-(2 trifluoromethoxy)phenyl] cyclohexylamino}ethyl amine hydrochloride was added 124 mg (0.34 mmole) of (+)-4-(3,4-difluorophenyl)-2-oxo oxazolidine-3-carboxylic acid 4-nitrophenyl ester and 124 gtL (1.2 mmole) 10 of diisopropylethylamine at ambient temperature. The homogeneous reaction mixture was stirred for 30 minutes, concentrated in vacuo and and residue was dissolved in ethyl acetate. The ethyl acetate solution was washed with 10% potassium carbonate solution (6X), dried (magnesium sulfate) and concentrated to give the crude product as an 15 oil. Flash column chromatography of the reaction product on silica gel (methanol/methylene chloride/ammonium hydroxide, gradient elution (0.5 to 2%)) afforded the title compound in analytically pure form: HPLC = 96% pure at 215 nm NMR(CDC13, 400 MHz): Consistent with structure 20 assignment and confirms presence of solvent. FAB MS: 553 (M + + 1). Analysis for C26H25F5N404*0.55H20: Calculated: C, 55.52; H, 4.68; N, 9.96. Found: C, 55.54; H, 4.64; N, 10.01. 25 - 59- WO 98/57632 PCT/US98/12573 EXAMPLE 20 0 H OO

OCF

3 H N H 0 ON F F F 4-(3,4-difluoro-phenyl)-2-oxo-oxazolidine-3-carboxylic acid {2-[4 cvano-4-(2-trifluoromethoxy-phenvl)-cyclohexvlaminol-ethyll-amide 5 Using reaction conditions identical to those described in Example 21, 126 mg (0.35 mmole) of (+)-4-(3,4-difluorophenyl)-2-oxo oxazolidine-3-carboxylic acid 4-nitrophenyl ester in 5 mL of N,N dimethylformamide was converted to the title compound with 139 mg (0.35 mmole) of 2- {[4-cyano-4-(2-trifluoromethoxy)phenyl] 10 cyclohexylamino}ethyl amine hydrochloride and 211 gL (1.2 mmole) of diisopropylethylamine. Extractive workup, followed by flash chromatography of the crude reaction product, and lyophilization gave a white solid: HPLC = >99% pure at 215 nm 15 NMR(CDC13, 400 MHz): Consistent with structure assignment. FAB MS: 553 (M + + 1). Analysis for C26H25F5N404: Calculated: C, 56.52; H, 4.56; N, 10.14. 20 Found: C, 56.76; H, 4.72; N, 10.18. - 60- WO 98/57632 PCT/US98/12573 EXAMPLE 21

CF

3 H +0 H H -O N N CN H0 F F 4-(3,4-difluoro-phenyl)-2-oxo-oxazolidine-3-carboxylic acid {2-[4 cyano-4-(2-trifluoromethyl-phenyl)-cyclohexylamino]-ethyll-amide 5 Using reaction conditions identical to those described in Example 21, 122 mg (0.34 mmole) of (+)-4-(3,4-difluorophenyl)-2-oxo oxazolidine-3-carboxylic acid 4-nitrophenyl ester in 5 mL of N,N dimethylformamide was converted to the title compound by reacting it with 129 mg (0.34 mmole) of 2-{[4-cyano-4-(2-trifluoromethyl)phenyl] 10 cyclohexylamino}ethyl amine hydrochloride and 204 gL (1.2 mmole) of diisopropylethylamine. Extractive workup, followed by flash chromatography of the crude reaction product, and lyophilization gave the title compound as a white powder: HPLC = 99% pure at 215 nm 15 NMR(CDC13, 400 MHz): Consistent with structure assignment. FAB MS: 537 (M + + 1). Analysis for C26H25F5N403: Calculated: C, 58.21; H, 4.70; N, 10.44. 20 Found: C, 57.70; H, 4.55; N, 10.32. - 61- WO 98/57632 PCT/US98/12573 EXAMPLE 22 O0

CF

3 HN' N N H CN F F 4-(3,4-difluoro-phenyl)-2-oxo-oxazolidine-3-carboxylic acid {2-[4 cyano-4-(2-trifluoromethyl-phenv1)-cyclohexylaminol-ethyll-amide 5 Using reaction conditions identical to those described in Example 21, 130 mg (0.36 mmole) of (+)-4-(3,4-difluorophenyl)-2-oxo oxazolidine-3-carboxylic acid 4-nitrophenyl ester in 5 mL of N,N dimethylformamide was converted to the title compound by reacting it with 138 mg (0.36 mmole) of 2-{[4-cyano-4-(2-trifluoromethyl)phenyl] 10 cyclohexylamino}ethyl amine hydrochloride and 219 pLL (1.26 mmole) of diisopropylethylamine. Extractive workup, followed by flash chromatography of the crude reaction product, and lyophilization gave the title compound as a white powder: HPLC = 97% pure at 215 nm 15 NMR(CDC13, 400 MHz): Consistent with structure assignment. FAB MS: 537 (M + + 1). Analysis for C26H25F5N403: Calculated: C, 58.21; H, 4.70; N, 10.44. 20 Found: C, 57.80; H, 4.61; N, 10.42. - 62- WO 98/57632 PCT/US98/12573 EXAMPLE 23 °0 H OO N N N

SO

2

CH

3 H0 F F (2- {[4-(3,4-difluoro-phenyl)-2-oxo-oxazolidine-3-carbonyl]-amino} ethyl)-(4-methanesulfonyl-4-phenyl-cyclohexyl)-ammonium chloride 5 Using reaction conditions identical to those described in Example 21, 172 mg (0.47 mmole) of (+)-4-(3,4-difluorophenyl)-2-oxo oxazolidine-3-carboxylic acid 4-nitrophenyl ester in a solvent mix of 8 mL of N,N-dimethylformamide and 2 mL of tetrahydrofuran was converted to the title compound by reacting it with 175 mg (0.47 mmole) of 2-[(4 10 methansulfonyl-4-phenyl) cyclohexylamino]ethyl amine hydrochloride and 288 pL (1.66 mmole) of diisopropylethylamine. Extractive workup, followed by flash chromatography of the crude reaction product (silica gel; methanol/methylene chloride elution, 1:1), and lyophilization gave the title compound which was converted to its HC1 salt with HC1 in 15 dioxane: HPLC = >93% pure at 215 nm NMR(CDC13, 400 MHz): Consistent with structure assignment and confirms presence of solvent. FAB MS: 522 (M + + 1). 20 Analysis for C25H29F2N305S*0.95 H20: Calculated: C, 52.20; H, 5.59; N, 7.31. Found: C, 52.19; H, 5.28; N, 7.22. - 63- WO 98/57632 PCT/US98/12573 EXAMPLE 24 0 | HN N N H

SO

2

CH

3 F F (2- {1[4-(3,4-difluoro-phenyl)-2-oxo-oxazolidine-3-carbonyl]-amino} ethyl)-(4-methanesulfonvl-4-phenvl-cyclohexvl)-ammonium chloride 5 Using reaction conditions identical to those described in Example 21, 153 mg (0.42 mmole) of (+)-4-(3,4-difluorophenyl)-2-oxo oxazolidine-3-carboxylic acid 4-nitrophenyl ester in 2 mL of N,N dimethylformamide was converted to the title compound by reacting it with 155 mg (0.42 mmole) of 2-[(4-methansulfonyl-4-phenyl) 10 cyclohexylamino]ethyl amine hydrochloride and 256 gtL (1.47 mmole) of diisopropylethylamine. Extractive workup, followed by flash chromatography of the crude reaction product (silica gel; methanol/methylene chloride/ammonium hydroxide gradient elution), and lyophilization gave the title compound which was converted to its 15 HC1 salt with HC1 in dioxane: HPLC = >93% pure at 215 nm NMR(CDC13, 400 MHz): Consistent with structure assignment and confirms presence of solvent. FAB MS: 522 (M + + 1). 20 Analysis for C25H29F2N305S 0. 5 H20: Calculated: C, 52.95; H, 5.51; N, 7.41. Found: C, 53.14; H, 5.80; N, 7.02. Utilizing the methodology described in detail in the 25 Examples and schemes above, the following compounds shown in Table 1 were made. -64- WO 98/57632 PCTIUS98/12573 N41 C)) -n -I >) 0 Aww o CIC c - W w 0 >

S-

WO 98/57632 PCT/US98/12573 \/ \/ 00 rA on -n CA 30 > t~tv o n

-

a .5 ?0 z 2 z -66- WO 98/57632 PCT/US98/12573 0 0/

-

a Co zz / O zz - n - n o - 2. 0a a PPP W 00 2 o 2 Tm o 0o 0s ^g2 zz-- Czs5 our ou -67 ~In t,.) OOQ% Q% -67 WO 98/57632 PCT/US98/12573 00 S I -I -n CAA C,. .

00 > o 0o cn

P

-68- WO 98/57632 PCT/US98/12573 zz zz *n oo > 0 00 00 cun oCm > .4i n1@ 4 seem as liii ~'lip zz 0 o ft a LALA II B o- II II .5 0% * 9@ LA LA 'L 000% C1a -69- WO 98/57632 PCT/US98/12573 0 0 LA >A LAA CCD Loa LA CA nz nC LA 0 *LhJ LA IIAi ~LA 9 ±11 -70- WO 98/57632 PCTIUS98/12573 0 I bo 7.0 A CA - 0 LA r a00 -1 0 no ??' .Pa* - Lo -71- WO 98/57632 PCT/US98/12573 0 = m zzx O O • *. ." + 0 0 00 '0 0 C) n )m -72

C.

1 A LitJ z z}= 00 It jt -72 0%4: -72- WO 98/57632 PCT/US98/12573 EXAMPLE 25 As a specific embodiment of an oral composition, 100 mg of the compound of Example 5 is formulated with sufficient finely divided 5 lactose to provide a total amount of 580 to 590 mg to fill a size 0 hard gel capsule. EXAMPLE 26 10 Screening assay: Alpha la Adrenergic Receptor Binding Membranes prepared from the stably transfected human alpha la cell line (ATCC CRL 11140) were used to identify compounds that bind to the human alpha la adrenergic receptor. These competition binding reactions (total volume = 200 pl) contained 50 mM Tris-HC1 pH. 15 7.4, 5 mM EDTA, 150 mM NaC1, 100 pM [125 I]-HEAT, membranes prepared from the alpha la cell line and increasing amounts of unlabeled ligand. Reactions were incubated at room temperature for one hour with shaking. Reactions were filtered onto Whatman GF/C glass fiber filters with a Inotec 96 well cell harvester. Filters were 20 washed three times with ice cold buffer and bound radioactivity was determined (Ki). Representative compounds of the present invention were found to have Ki values < 50 nM. EXAMPLE 27 25 Selective Binding assays Membranes prepared from stably transfected human alpha ld and alpha lb cell lines (ATCC CRL 11138 and CRL 11139, respectively) were used to identify compounds that selectively bind to the human 30 alpha la adrenergic receptor. These competition binding reactions (total volume = 200 Wl) contained 50 mM Tris-HC1 pH. 7.4, 5 mM EDTA, 150 mM NaC1, 100 pM [125 I]-HEAT, membranes prepared from cell lines transfected with the respective alpha 1 subtype expression plasmid and increasing amounts of unlabeled ligand. Reactions were incubated at 35 room temperature for one hour with shaking. Reactions were filtered - 73- WO 98/57632 PCT/US98/12573 onto Whatman GF/C glass fiber filters with a Inotec 96 well cell harvester. Filters were washed three times with ice cold buffer and bound radioactivity was determined (Ki). 5 EXAMPLE 28 Exemplary Counterscreens 1. Assay Title: Dopamine D2, D3, D4 in vitro screen 10 Objective of the Assay: The objective of this assay is to eliminate agents which specifically affect binding of [3H] spiperone to cells expressing human dopamine receptors D2, D3 or D4. 15 Method: Modified from VanTol et al (1991); Nature (Vol 350) Pg 610 613. Frozen pellets containing specific dopamine receptor 20 subtypes stably expressed in clonal cell lines are lysed in 2 ml lysing buffer (10mM Tris-HC1/5mM Mg, pH 7.4). Pellets obtained after centrifuging these membranes (15' at 24,450 rpm) are resuspended in 50mM Tris-HC1 pH 7.4 containing EDTA, MgCl[2], KC1, NaC1, CaCl[2] and ascorbate to give a 1 Mg/mL suspension. The assay is initiated by 25 adding 50-75 pg membranes in a total volume of 500 pl containing 0.2 nM [3H]-spiperone. Non-specific binding is defined using 10 RfM apomorphine. The assay is terminated after a 2 hour incubation at room temperature by rapid filtration over GF/B filters presoaked in 0.3% PEI, using 50mM Tris-HC1 pH 7.4. 30 2. Assay Title: Serotonin 5HT1a Objective of the Assay The objective of this assay is to eliminate agents which 35 specifically affect binding to cloned human 5HT1a receptor - 74- WO 98/57632 PCT/US98/12573 Method: Modified from Schelegel and Peroutka Biochemical Pharmacology 35: 1943-1949 (1986). 5 Mammalian cells expressing cloned human 5HT1a receptors are lysed in ice-cold 5 mM Tris-HC1 , 2 mM EDTA (pH 7.4) and homogenized with a polytron homogenizer. The homogenate is centrifuged at 1000Xg for 30', and then the supernatant is centrifuged again at 38,000Xg for 30'. The binding assay contains 0.25 nM [3H]8-OH 10 DPAT (8-hydroxy-2-dipropylamino-1,2,3,4-tetrahydronaphthalene) in 50 mM Tris-HC1, 4 mM CaC12 and lmg/ml ascorbate. Non-specific binding is defined using 10 RM propranolol. The assay is terminated after a 1 hour incubation at room temperature by rapid filtration over GF/Cfilters 15 EXAMPLE 29 Exemplary Functional Assays In order to confirm the specificity of compounds for the 20 human alpha la adrenergic receptor and to define the biological activity of the compounds, the following functional tests may be performed: 1. In vitro Rat, Dog and Human Prostate and Dog Urethra Taconic Farms Sprague-Dawley male rats, weighing 250 25 400 grams are sacrificed by cervical dislocation under anesthesia (methohexital; 50 mg/kg, i.p.). An incision is made into the lower abdomen to remove the ventral lobes of the prostate. Each prostate removed from a mongrel dog is cut into 6-8 pieces longitudinally along the urethra opening and stored in ice-cold oxygenated Krebs solution 30 overnight before use if necessary. Dog urethra proximal to prostate is cut into approximately 5 mm rings, the rings are then cut open for contractile measurement of circular muscles. Human prostate chips from transurethral surgery of benign prostate hyperplasia are also stored overnight in ice-cold Krebs solution if needed. - 75- WO 98/57632 PCT/US98/12573 The tissue is placed in a Petri dish containing oxygenated Krebs solution [NaC1, 118 mM; KC1, 4.7 mM; CaC12, 2.5 mM; KH2PO4, 1.2 mM; MgSO4, 1.2 mM; NaHCO3, 2.0 mM; dextrose, 11 mM] warmed to 37 0 C. Excess lipid material and connective tissue are carefully 5 removed. Tissue segments are attached to glass tissue holders with 4-0 surgical silk and placed in a 5 ml jacketed tissue bath containing Krebs buffer at 37 0 C, bubbled with 5% C02/95% 02. The tissues are connected to a Statham-Gould force transducer; 1 gram (rat, human) or 1.5 gram (dog) of tension is applied and the tissues are allowed to equilibrate for 10 one hour. Contractions are recorded on a Hewlett-Packard 7700 series strip chart recorder. After a single priming dose of 3 gM (for rat), 10 RM (for dog) and 20 pM (for human) of phenylephrine, a cumulative concentration response curve to an agonist is generated; the tissues are washed every 15 10 minutes for one hour. Vehicle or antagonist is added to the bath and allowed to incubate for one hour, then another cumulative concentration response curve to the agonist is generated. EC50 values are calculated for each group using GraphPad Inplot software. pA2 (-log Kb) values were obtained from Schild plot 20 when three or more concentrations were tested. When less than three concentrations of antagonist are tested, Kb values are calculated according to the following formula Kb = IB, x-1 where x is the ratio of EC50 of agonist in the presence and absence of 25 antagonist and [B] is the antagonist concentration. 2. Measurement of Intra-Urethral Pressure in Anesthetized Does Purpose: Benign prostatic hyperplasia causes a decreased urine flow 30 rate that may be produced by both passive physical obstruction of the prostatic urethra from increased prostate mass as well as active obstruction due to prostatic contraction. Alpha adrenergic receptor antagonists such as prazosin and terazosin prevent active prostatic contraction, thus improve urine flow rate and provide symptomatic 35 relief in man. However, these are non-selective alpha 1 receptor - 76- WO 98/57632 PCT/US98/12573 antagonists which also have pronounced vascular effects. Because we have identified the alpha la receptor subtype as the predominent subtype in the human prostate, it is now possible to specifically target this receptor to inhibit prostatic contraction without concomitant changes in 5 the vasculature. The following model is used to measure adrenergically mediated changes in intra-urethral pressure and arterial pressure in anesthetized dogs in order to evaluate the efficacy and potency of selective alpha adrenergic receptor antagonists. The goals are to: 1) identify the alpha 1 receptor subtypes responsible for prostatic/urethral 10 contraction and vascular responses, and 2) use this model to evaluate novel selective alpha adrenergic antagonists. Novel and standard alpha adrenergic antagonists may be evaluated in this manner. Methods: Male mongrel dogs (7-12 kg) are used in this study. The 15 dogs are anesthetized with pentobarbital sodium (35 mg/kg, i.v. plus 4 mg/kg/hr iv infusion). An endotracheal tube is inserted and the animal ventilated with room air using a Harvard instruments positive displacement large animal ventilator. Catheters (PE 240 or 260) are placed in the aorta via the femoral artery and vena cava via the femoral 20 veins (2 catheters, one in each vein) for the measurement of arterial pressure and the administration of drugs, respectively. A supra-pubic incision -1/2 inch lateral to the penis is made to expose the urethers, bladder and urethra. The urethers are ligated and cannulated so that urine flows freely into beakers. The dome of the bladder is retracted to 25 facilitate dissection of the proximal and distal urethra. Umbilical tape is passed beneath the urethra at the bladder neck and another piece of umbilical tape is placed under the distal urethra approximately 1-2 cm distal to the prostate. The bladder is incised and a Millar micro-tip pressure transducer is advanced into the urethra. The bladder incision 30 is sutured with 2-0 or 3-0 silk (purse-string suture) to hold the transducer. The tip of the transducer is placed in the prostatic urethra and the position of the Millar catheter is verified by gently squeezing the prostate and noting the large change in urethral pressure. Phenylephrine, an alpha 1 adrenergic agonist, is 35 administered (0.1-100 ug/kg, iv; 0.05 ml/kg volume) in order to construct - 77- WO 98/57632 PCT/US98/12573 dose response curves for changes in intra-urethral and arterial pressure. Following administration of increasing doses of an alpha adrenergic antagonist (or vehicle), the effects of phenylephrine on arterial pressure and intra-urethral pressure are re-evaluated. Four or 5 five phenylephrine dose-response curves are generated in each animal (one control, three or four doses of antagonist or vehicle). The relative antagonist potency on phenylephrine induced changes in arterial and intra-urethral pressure are determined by Schild analysis. The family of averaged curves are fit simultaneously (using ALLFIT software 10 package) with a four paramenter logistic equation constraining the slope, minimum response, and maximum response to be constant among curves. The dose ratios for the antagonist doses (rightward shift in the dose-response curves from control) are calculated as the ratio of the ED50's for the respective curves. These dose-ratios are then used to 15 construct a Schild plot and the Kb (expressed as ug/kg, iv) determined. The Kb (dose of antagonist causing a 2-fold rightward shift of the phenylephrine dose-response curve) is used to compare the relative potency of the antagonists on inhibiting phenylephrine responses for intra-urethral and arterial pressure. The relative selectivity is 20 calculated as the ratio of arterial pressure and intra-urethral pressure Kb's. Effects of the alpha 1 antagonists on baseline arterial pressure are also monitored. Comparison of the relative antagonist potency on changes in arterial pressure and intra-urethral pressure provide insight as to whether the alpha receptor subtype responsible for 25 increasing intra-urethral pressure is also present in the systemic vasculature. According to this method, one is able to confirm the selectivity of alpha la adrenergic receptor antagonists that prevent the increase in intra-urethral pressure to phenylephrine without any activity at the vasculature. 30 While the foregoing specification teaches the principles of the present invention, with examples provided for the purpose of illustration, it will be understood that the practice of the invention encompasses all of the usual variations, adaptations and/or - 78- WO 98/57632 PCT/US98/12573 modifications as come within the scope of the following claims and their equivalents. - 79-

Claims (24)

1. A compound of the formula: M2 n E RC N2< Q R R G L 5 wherein Q is selected from O \jR 8 R 8 O O R 90 O R9 , Rio , (X)q R19R3 R R 7 O 1 0 R 1 1 12 ,N,xN N (X)7 W 00 Nq 0 .- ,0 - ' N \\ H 0 R 13 14 R 1 3 0 0 N 4 - 80- WO 98/57632 PCT/US98/12573 C1-8 alkyl, C3-8 cycloalkyl, (CH2)0-40R 1 5 , (CH2)0-4N(R 1 6 )2, (CH2)0-4CN, (CH2)0-4CF3, (CH2)0-4CO2R 16 , (CH2)0-4CON(R 1 6 )2, (CH2)0-4SO2R 1 5 or (CH2)0-4SO2N(R 1 6 )2; 5 J is selected from hydrogen, C1-8 alkyl, C3-8 cycloalkyl, (CH2)1-40R 1 5 , (CH2)1-4N(R 1 6 )2, (CH2)1-4CN, (CH2)0-4CF3, (CH2)0-4CO2R 1 6 , (CH2)0-4CON(R 1 6 )2, (CH2)0-4SO2R 1 5 or (CH2)0-4SO2N(R 1 6 )2; 10 R 1 is selected from unsubstituted, mono- or poly-substituted phenyl wherein the substitutents on the phenyl are independently selected from halogen, CF3, cyano, nitro, N(R 1 6 )2, NR 1 6 COR 1 8 , NR 1 6 CON(R 1 8 )2, NR 16 SO2R 1 8 , NR 1 6 SO2N(R 1 8 )2, OR 1 5 , (CH2)0-4CO2R 1 6 , (CH2)0-4CON(R 1 6 )2, (CH2)0-4SO2N(R 1 6 )2, (CH2)0-4SO2R 1 5 or 15 C1-4 alkyl; or unsubstituted, mono- or poly-substituted pyridyl, pyrazinyl, thienyl, thiazolyl, furanyl, quinazolinyl or naphthyl wherein the substituents on the pyridyl, pyrazinyl, thienyl, thiazolyl, furanyl, quinazolinyl or naphthyl are independently selected from CF3, cyano, nitro, N(R 1 6 )2, (CH2)0-4CO2R 1 6 , (CH2)0-4CON(R1 6 )2, 20 (CH2)0-4SO2N(R 1 6 )2, (CH2)0-4SO2R 15 , phenyl, OR 1 5 , halogen, C1-4 alkyl or 3-8 cycloalkyl; R is selected from hydrogen, cyano, OR 15 , CO2R 1 5 , CON(R 1 6 )2, SO2R 1 5 , SO2N(R1 6 )2, tetrazole, isooxadiazole, unsubstituted, mono- or poly 25 substituted phenyl wherein the substitutents on the phenyl are independently selected from halogen, cyano, OR 1 5 , (CH2)0-4CO2R 1 6 , (CH2)0-4CON(R 1 6 )2, N(R 1 6 )2, NR 1 6 COR 1 5 , NR 1 6 CON(R 1 8 )2, NR 1 6 SO2R 1 5 , NR 1 6 SO2N(R 1 8 )2, (CH2)0-4SO2N(R 1 6 )2, (CH2)0-4SO2R 1 5 or C1-4 alkyl; or unsubstituted, mono- or poly 30 substituted pyridyl, thienyl, furanyl or naphthyl wherein the substituents on the pyridyl, thienyl, furanyl or naphthyl are independently selected from CF3, (CH2)0-4CO2R 1 6 , (CH2)0-4CON(R 1 6 )2, (CH2)0-4SO2N(R 1 6 )2, (CH2)0-4SO2R 1 5 , phenyl, OR 1 5 , halogen, C1-4 alkyl or C3-8 cycloalkyl; -81- WO 98/57632 PCT/US98/12573 R 2 and R 7 are each independently selected from hydrogen, C1-8 alkyl, C4-8 cycloalkyl, (CH2)0-4CO2R 1 6 , (CH2)0-4CON(R 1 6 )2, (CH2)0-4COR 1 5 , (CH2)2-40R 1 5 , (CH2)1-4CF3, (CH2)0-4SO2R 1 5 , 5 (CH2)0-4SO2N(R 1 6)2 or (CH2)1-4CN; R 3 , R 6 , R 8 , R 9 and R 1 0 are each independently selected from hydrogen, C1-8 alkyl, C3-8 cycloalkyl, (CH2)1-40R 1 5 or (CH2)0-4CF3; 10 R 1 1 and R 1 2 are each independently selected from hydrogen, C1-8 alkyl or C3-8 cycloalkyl; R 1 3 and R 1 4 are each independently selected from hydrogen, C1-8 alkyl, 15 C3-8 cycloalkyl, (CH2)0-40R 1 5 , (CH2)0-4CF3, unsubstituted, mono- or poly-substituted phenyl wherein the substitutents on the phenyl are independently selected from halogen, CF3, cyano, nitro, CO2R 1 6 , OR 1 5 , (CH2)0-4CON(R 1 6 )2, (CH2)0-4CO2R 1 6 or C1-4 alkyl; or unsubstituted, mono- or poly-substituted: pyridyl, thienyl, 20 furanyl or naphthyl wherein the substituents on the pyridyl, thienyl, furanyl or naphthyl are independently selected from CF3, phenyl, OR 15 , halogen, C1-4 alkyl or C3-8 cycloalkyl; R 1 5 is selected from hydrogen, C1-8 alkyl, C3-8 cycloalkyl or 25 (CH2)0-4CF3; R 1 6 and R 1 8 are each independently selected from hydrogen, C1-8 alkyl, C3-8 cycloalkyl or (CH2)1-4CF3; 30 R 1 9 is selected from hydrogen, 01-8 alkyl, C3-8 cycloalkyl, (CH2)0-40R 1 5 or (CH2)0-4CF3; W is O or NR 1 1 ; - 82- WO 98/57632 PCT/US98/12573 each X is independently selected from halogen, cyano, nitro, C1-8 alkyl, C3-8 cycloalkyl, (CH2)0-40R 2 4 or (CH2)0-4CF3; R 2 4 is selected from hydrogen, C 1 _ 8 alkyl, C 3 - 8 cycloalkyl or (CH 2 )04CF 3 ; 5 Y is C-R 1 5 or N; Z is hydrogen, oxygen or sulphur; 10 m, n, p and q are each independently an integer from zero to four; o is an integer from one to four; r is zero or one; and the pharmaceutically acceptable salts thereof. 15

2. The compound of Claim 1, of the formula M n E R2 R N Q I- G L G wherein 20 E, G, L, M and J are each independently selected from hydrogen, C1-8 alkyl, C3-8 cycloalkyl, (CH2)0-4CO2R 1 6 or (CH2)0-4CF3; R 1 is selected from unsubstituted, mono-, di- or tri-substituted phenyl wherein the substitutents on the phenyl are independently selected from 25 halogen, CF3, cyano, nitro, N(R 1 6 )2, NR 1 6 COR 1 8 , NR 1 6 CON(R 1 8 )2, NR 16 SO2R 1 8 , NR 1 6 SO2N(R 1 8 )2, OR 1 5 , (CH2)0-4CO2R 1 6 , (CH2)0-4CON(R 1 6 )2, (CH2)0-4SO2N(R 1 6 )2, (CH2)0-4SO2(R 1 5 )2 or C1-4 alkyl; or unsubstituted, mono-, di- or tri-substituted pyridyl, pyrazinyl, thienyl, thiazolyl, furanyl, quinazolinyl or naphthyl wherein - 83- WO 98/57632 PCT/US98/12573 the substituents on the pyridyl, pyrazinyl, thienyl, thiazolyl, furanyl, quinazolinyl or naphthyl are independently selected from CF3, cyano, nitro, N(R 1 6 )2, (CH2)0-4CO2R 1 6 , (CH2)0-4CON(R 1 6 )2, (CH2)0-4SO2N(R1 6 )2, (CH2)0-4SO2(R 1 5 )2, phenyl, OR 1 5 , halogen, C1-4 5 alkyl or C3-8 cycloalkyl; R is selected from hydrogen, cyano, OR 1 5 , CO2R 1 5 , CON(R 1 6 )2, SO2R 1 5 , SO2N(R 1 6 )2 or unsubstituted, mono- or di-substituted phenyl wherein the substitutents on the phenyl are independently selected from 10 halogen, cyano, OR 1 5 , (CH2)0-4CO2R 1 6 , (CH2)0-4CON(R 1 6 )2, N(R 1 6 )2, NR 16 COR 1 5 , NR 1 6 CON(R 18 )2, NR 1 6 SO2R 1 5 , NR 1 6 SO2N(R 1 8 )2, (CH2)0 4SO2N(R 1 6)2, (CH2)0-4SO2Rl 5 or C1-4 alkyl; or unsubstituted, mono- or di-substituted pyridyl, thienyl, furanyl or naphthyl wherein the substituents on the pyridyl, thienyl, 15 furanyl or naphthyl are independently selected from CF3, (CH2)0-4CO2R 1 6 , (CH2)0-4CON(R1 6 )2, (CH2)0-4SO2N(R 1 6 )2, (CH2)0 4SO2R 1 5 , phenyl, OR 15 , halogen, C1-4 alkyl or C3-8 cycloalkyl; 20 R 2 and R 7 are each independently selected from hydrogen, C1-8 alkyl, C4-8 cycloalkyl or (CH2)1-4CF3; R 1 3 and R 1 4 are each independently selected from hydrogen, C1-8 alkyl, C3-8 cycloalkyl, (CH2)1-40R 1 5 , (CH2)0-4CF3, unsubstituted, 25 mono-, di- or tri-substituted phenyl wherein the substitutents on the phenyl are independently selected from halogen, CF3, cyano, nitro, CO2R 16 , OR 1 5 , (CH2)0-4CON(R 1 6 )2, (CH2)0-4CO2R 1 6 or C1-4 alkyl; or unsubstituted, mono-, di- or tri substituted: pyridyl, thienyl, furanyl or naphthyl wherein the 30 substituents on the pyridyl, thienyl, furanyl or naphthyl are independently selected from CF3, phenyl, OR 1 5 , halogen, C1-4 alkyl or C3-8 cycloalkyl; n is an integer from zero to two; and 35 o is an integer from one to four; -84- WO 98/57632 PCT/US98/12573 and the pharmaceutically acceptable salts thereof.

3. The compound of Claim 2, of the formula S E R 2 R N-(CH 2 ) 5 -Q 5Rb wherein Q is selected from R8 N N R9 R70. O tl RO O Ri R R 19 R13 0 (X)q 0 / or 0 0 E and J are each independently selected from hydrogen or C02-C1-6 alkyl; 10 R 1 is selected from unsubstituted, mono-, di- or tri-substituted phenyl wherein the substitutents on the phenyl are independently selected from halogen, CF3, cyano, nitro, N(R 1 6 )2, OR 1 5 , (CH2)0-2CO2R 1 6 , (CH2)0-2CON(R 1 6 )2 or C1-4 alkyl; or unsubstituted, mono- or di 15 substituted pyridyl wherein the substitutents on the pyridyl are independently selected from halogen, CF3, cyano, nitro, N(R 1 6 )2, OR 1 5 , (CH2)0-2CO2R 16 , (CH2)0-2CON(R 1 6)2 or C1-4 alkyl; - 85- WO 98/57632 PCT/US98/12573 R is selected from hydrogen, cyano, OR 1 5 , CO2R 1 5 , CON(R 1 6 )2, SO2R 15 or SO2N(R 1 6 )2; R 2 and R 7 are each independently selected from hydrogen, 01-6 alkyl, 5 C4-6 cycloalkyl or (CH2)1-4CF3; R 8 , R 9 and R 1 0 are each independently selected from hydrogen, C1-6 alkyl, C3-6 cycloalkyl, (CH2)2-40R 1 5 or (CH2)0-2CF3; 10 R 1 3 is selected from hydrogen, C1-6 alkyl, C3-6 cycloalkyl, (CH2)2-40R 1 5 , (CH2)0-2CF3, or unsubstituted, mono-, or di- substituted phenyl wherein the substitutents on the phenyl are independently selected from halogen, CF3, cyano, CO2R 1 6 , OR 1 5 or C1-4 alkyl; 15 R 1 5 is selected from hydrogen, C1-6 alkyl, C3-6 cycloalkyl or (CH2)0-2CF3; R 1 6 is selected from hydrogen, C1-6 alkyl, C3-6 cycloalkyl or (CH2)1-2CF3; 20 R 1 9 is selected from hydrogen, C1-6 alkyl, C3-6 cycloalkyl, (CH2)0-40R 1 5 or (CH2)0-2CF3; each X is independently selected from halogen or 01-4 alkyl, 25 p is an integer from zero to two; q is an integer from zero to three; and the pharmaceutically acceptable salts thereof. 30

4. The compound of Claim 3, of the formula H (R 17 )s R - 86- WO 98/57632 PCT/US98/12573 wherein Q is o(X)q NNq 0 HO A is C-R 1 7 or N; 5 R is selected from hydrogen, cyano, hydroxy, C02R 1 5 , CON(R 1 6 )2, SO2R 1 5 or SO2N(R 16 )2; each R 1 7 is independently selected from hydrogen, halogen, C02R 1 6 , cyano, nitro, CON(R 1 6 )2, SO2R 15 , SO2N(R 1 6 )2 or OR 1 5 ; 10 each X is independently selected from flourine or methyl; and s is an integer from zero to two; 15 and the pharmaceutically acceptable salts thereof.

5. The compound of Claim 3, selected from (4-cyano-4-phenyl-cyclohexyl)-[3-(2,2-di-p-tolyl-acetylamino)-propyl] 20 methyl-ammonium chloride; (4-cyano-4-phenyl-cyclohexyl)-methyl-[4-(1,1,3-trioxo-1,3-dihydro-116 benzo[d]isothiazol-2-yl)-butyl]-ammonium chloride; 25 (4-cyano-4-phenyl-cyclohexyl)-methyl-[3-(1,1,3-trioxo-1,3-dihydro-116 benzo[d]isothiazol-2-yl)-propyl]-ammonium chloride; - 87- WO 98/57632 PCT/US98/12573 (+)-2-Oxo-4-(3,4,5-trifluorophenyl)-oxazolidine-3-carboxylic acid [2-(4 cyano-4-phenyl-cyclohexylamino)-ethyl] amide; (+)-2-Oxo-4-(3,4-difluorophenyl)-oxazolidine-3-carboxylic acid 5 [2-(4-phenyl-4-methoxycarbonyl-cyclohexylamino)-ethyl] amide; (+)-cis-2-Oxo-4-(3,4-difluorophenyl)-oxazolidine-3-carboxylic acid [2-(4-Cyano-4-(2-ethoxyphenyl)-cyclohexylamino)-ethyl] amide; 10 [4-cyano-4-(2-methoxy-phenyl)-cyclohexyl]-(2- {[4-(3,4-difluoro-phenyl) -2 oxo-oxazolidine-3-carbonyl]-amino}-ethyl)-ammonium chloride; [4-cyano-4-(2-fluoro-phenyl)-cyclohexyl]-(2- {[4-(3,4-difluoro-phenyl)-2-oxo oxazolidine-3-carbonyl]-amino}-ethyl)- ammonium chloride; 15 4-(3,4-difluoro-phenyl)-2-oxo-oxazolidine-3-carboxylic acid {2-[4-cyano-4 (2-trifluoromethoxy-phenyl)-cyclohexylamino]-ethyl}-amide; 4-(3,4-difluoro-phenyl)-2-oxo-oxazolidine-3-carboxylic acid {2-[4-cyano-4 20 (2-trifluoromethoxy-phenyl)-cyclohexylamino]-ethyl}-amide; 4-(3,4-difluoro-phenyl)-2-oxo-oxazolidine-3-carboxylic acid {2-[4-cyano-4 (2-trifluoromethyl-phenyl)-cyclohexylamino]-ethyl}-amide; 25 4-(3,4-difluoro-phenyl)-2-oxo-oxazolidine-3-carboxylic acid {2-[4-cyano-4 (2-trifluoromethyl-phenyl)-cyclohexylamino]-ethyl}-amide; (2- {[4-(3,4-difluoro-phenyl)-2-oxo-oxazolidine-3-carbonyl]-amino}-ethyl) (4-methanesulfonyl-4-phenyl-cyclohexyl)-ammonium chloride; or 30 (2-{[4-(3,4-difluoro-phenyl)-2-oxo-oxazolidine-3-carbonyl]-amino}-ethyl) (4-methanesulfonyl-4-phenyl-cyclohexyl)-ammonium chloride and the pharmaceutically acceptable salts thereof. 35 - 88- WO 98/57632 PCT/US98/12573

6. A pharmaceutical composition comprising the compound of Claim 1 and a pharmaceutically acceptable carrier.

7. A pharmaceutical composition made by combining a 5 compound of Claim 1 and a pharmaceutically acceptable carrier.

8. A process for making a pharmaceutical composition comprising combining a compound of Claim 1 and a pharmaceutically acceptable carrier. 10

9. The composition of Claim 6 further comprising a testosterone 5-alpha reductase inhibitor.

10. The composition of Claim 9, wherein the testosterone 15 5-alpha reductase inhibitor is a type 1, a type 2, both a type 1 and a type 2 or a dual type 1 and type 2 testosterone 5-alpha reductase inhibitor.

11. The composition of Claim 10, wherein the testosterone 5-alpha reductase inhibitor is a type 2 testosterone 5-alpha 20 reductase inhibitor.

12. The composition of Claim 11, wherein the testosterone 5-alpha reductase inhibitor is finasteride. 25

13. A method of treating benign prostatic hyperplasia in a subject in need thereof which comprises administering to the subject a therapeutically effective amount of the compound of Claim 1.

14. The method of Claim 13, wherein the compound 30 additionally does not cause a fall in blood pressure at dosages effective to alleviate benign prostatic hyperlasia.

15. The method of Claim 14, wherein the compound is administered in combination with a testosterone 5-alpha reductase 35 inhibitor. - 89- WO 98/57632 PCT/US98/12573

16. The method of Claim 15, wherein the testosterone 5 alpha reductase inhibitor is finasteride. 5

17. A method of treating benign prostatic hyperplasia in a subject in need thereof which comprises administering a therapeutically effective amount of the composition of Claim 6.

18. The method of Claim 17, wherein the composition 10 further comprises a therapeutically effective amount of a testosterone 5 alpha reductase inhibitor.

19. A method of relaxing lower urinary tract tissue in a subject in need thereof which comprises administering to the subject a 15 therapeutically effective amount of the compound of Claim 1.

20. The method of Claim 19, wherein the compound additionally does not cause a fall in blood pressure at dosages effective to relax lower urinary tract tissue. 20

21. The method of Claim 19, wherein the compound is administered in combination with a testosterone 5-alpha reductase inhibitor. 25

22. The method of Claim 21, wherein the testosterone 5 alpha reductase inhibitor is finasteride.

23. A method of treating a condition which is susceptible to treatment by antagonism of the alpha la receptor which comprises 30 administering to a subject in need thereof an amount of the compound of Claim 1 effective to treat the condition.

24. A method of eliciting an alpha la antagonizing effect in a mammal in need thereof, comprising administering to the 35 mammal a therapeutically effective amount of the compound of Claim 1. - 90-