AU760434B2 - Amide compounds - Google Patents

Description

WO 00/42011 PCT/JP00/00017

DESCRIPTION

AMIDE COMPOUNDS TECHNICAL FIELD This invention relates to new amide compounds and pharmaceutically acceptable salts thereof which are useful as a medicament.

BACKGROUND ART Some aminopiperazine derivatives have been known as useful anti-amnesia or anti-dementia agents, for example, in PCT International Publication Nos. WO 91/01979 and WO 98/35951.

DISCLOSURE OF INVENTION This invention relates to new amide compounds and pharmaceutically acceptable salts thereof.

More particularly, it relates to new amide compounds and pharmaceutically acceptable salts thereof which have the potentiation of the cholinergic activity, to processes for the preparation thereof, to a pharmaceutical composition comprising the same, and to a method for the treatment and/or prevention of disorders in the central nervous system for mammals, and more particularly to method for the treatment and/or prevention of amnesia, dementia senile dementia, Alzheimer's dementia, dementia associated with various diseases such as cerebral vascular dementia, cerebral posttraumatic dementia, dementia due to brain tumor, dementia due to chronic subdural hematoma, dementia due to normal pressure hydrocephalus, post-meningitis dementia, Parkinson's disease type dementia, etc.), and the like. Additionally, the object compound is expected to be useful as therapeutical and/or preventive agents for schizophrenia, depression, stroke, head injury, nicotine withdrawal, spinal cord injury, anxiety, 2 pollakiuria, incontinence of urine, myotonic dystrophy, attention deficit hyperactivity disorder, excessive daytime sleepiness (narcolepsy), Parkinson's disease or autism.

One object of this invention is to provide new and useful amide compounds and pharmaceutically acceptable salts thereof which possess the potentiation of the cholinergic activity.

Another object of this invention is to provide processes for preparation of said amide compounds and salts thereof.

A further object of this invention is to provide a pharmaceutical composition comprising, as an active ingredient, said amide compounds and pharmaceutically acceptable salt thereof.

Still further object of this invention is to provide a therapeutic method for the treatment and/or prevention of aforesaid diseases in mammals, using said amide compounds and pharmaceutically acceptable salts thereof.

In general the amide compounds can be represented by the following general formula

E

R1-A-N EX-Y-Q-R 2 1 3 i4 oo* wherein R 1 is acyl,

R

2 is lower alkyl, lower alkoxy, lower alkylamino, lower alkenyl, lower alkenyloxy, lower alkenylamino, lower alkynyl, lower alkynyloxy, lower alkynylamino, cyclo(lower)alkyl, cyclo(lower)alkyloxy, cyclo(lower)alkylamino, aryl, aryloxy, arylamino, a heterocyclic group or amino substituted with a heterocyclic group, each of which may be substituted with suitable substituent(s); or acyl; WO 00/42011 jjirfk 3 rv I/JrU/UUUU7 0 A is a single bond, or -SO 2 E is lower alkylene optionally substituted with suitable substituent(s), X is CH or N,

R

Y is a single bond, lower alkylene or (wherein R 5 is hydrogen, lower alkyl, substituted-lower alkyl, an N-protective group, aryl, acyl or a heterocyclic group), 0 Q is -CH 2

-SO

2 or and

R

3 and R 4 are each hydrogen or lower alkyl, or are taken together to form lower alkylene optionally condensed with a cyclic hydrocarbon or a heterocyclic ring, provided that when X is N, then 1) Y is a single bond, and 0 Q is -CH 2 or -SO 2 or 2) Y is lower alkylene, and pharmaceutically acceptable salts thereof.

The object compound or its salt can be prepared by processes as illustrated in the following reaction schemes.

Process 1 R1-A-NE NH HO-Qa-R 2 k3 k4

[II]

[II]

or its reactive derivative or its salt at the carboxy or sulfo group, or a salt thereof WO 00/42011 WO 0042011PCT/JPOO/0001 7 4

E

R1-A-N~ ~NQa-R 2 1 k3 1 k4 [Ial or its salt R6-NCO [Ijv] R1-A-N Il EX NH k3 1

U

(III

or its salt Ri-A-N CH-NH2 i!3 4

[VI

or its salt 0

R

1 E N-6~H-R6 1 k3 1 k4 [Ib] or its salt HO-Qa-R 2

[III]

or its reactive derivative at the carboxy or sulfa group, or a salt thereof Rl-A-N,- E CH-NHQa-R 2 1 k3 !4 (Ic] or its salt WO 00/42011 PCT/JPOO/0001 7 E

R

6 -NCO [IV]E0 R1-A-N1- 1-CH-NH2 Rl-A-N' 'H-NHJNH-R6 IV]i or its salt [Id] or its salt Prcs HN EI1X-Y-Q-R 2

[VI]

or its salt

R

1

-A-OH

[VII]

or its reactive derivative at the carboxy or sulfa group, or a salt thereof Rl-A-N' X-Y-Q-R 2

[I]

or its salt Rl 1 A-NI XQa-OH 1 k3 1 k4 H2N-R 7

[VIII]

or its reactive derivative at the carboxy or sulfa group, or a salt thereof

[IX]

or its salt WO 00/42011 WO 0042011PCT/JPO/OO0i 7 6

R

1 A-N- ElX QaNH-R 7 or its salt Pocs,1 R-Qb-Za

R

E XI] E Rl-A-NII I CH-N-R5 10 A N ICN, Ib-R2 a RC b a [XJ [If] or its salt or its salt Process R 1 -A-NIIE'CHEbR b~ 1 k a elimination of the N-protective group E H Rl- i- H- -Q (if]I or its salt 1Ig] or its salt b-Zb -CH-N-Qc-R2 N H Q R 3 I4 b3 4a [Ihi or its salt [Ii] or its salt WO 00/42011 PCT/.Pnn/nnm 7 7 Process R1-A-NE NH Zc-Ya-Qa-R 2 R3 k4 [II]

[XIII]

or its salt

E

R

1 EN-Ya-Qa-R 2 3 4 [Ij] or its salt wherein R 1

R

2

R

3

R

4 A, E, Q, X and Y are each as defined above,

O

Qa is or -SO 2

R

6 is aryl which may be substituted with suitable substituent(s), or pyridyl,

R

7 is lower alkyl, lower alkenyl, lower alkynyl, cyclo(lower)alkyl, aryl or a heterocyclic group, each of which may be substituted with suitable substituent(s),

R

5 is an N-protective group, R is lower alkyl, lower alkenyl, lower alkynyl, cyclo(lower)alkyl, aryl or a heterocyclic group, each of which may be substituted with suitable substituent(s), 0 Qb is -CH 2 or -SO 2 Za is an acid residue, 0

O

Qc is l P -OPERVJgc\lnKM)4-i claims doc-.llI)3/i -8- Rb is lower alkyl, Zb is an acid residue, Zc is an acid residue, and Ya is lower alkylene.

More particularly, in a first aspect of the invention there is provided a compound of the general formula

E

R'-A-N

X-Y-Q-R

2 R R(I) wherein

R

1 is C1-C6 alkanoyl, Cl-C6 alkoxycarbonyl, benzoyl, benzoyl substituted with halo C1-C6 alkoxy, C 1

-C

6 alkylsulfonyl, phenylsulfonyl, phenylsulfonyl substituted with halogen, or cyclo C3-C6 alkylcarbonyl,

R

2 -is phenyl, phenyloxy or phenylamino, each phenyl of which may be substituted with halogen; pyridyl; or pyridylamino, 20 A is a single bond, E is ethylene, X is CH,

R

Y is -N-(wherein R 5 is hydrogen),

O

II

Q is -C-or -SO 2 and

.SR

3 and R 4 are taken together to form ethylene, ,and pharmaceutically acceptable salt thereof.

P:\OPERUgc\I 89O44M caims.doc4)3)03/03 -8A- In a second aspect of the invention there is provided a process for preparing a compound of the general formula

E

R1 A-N EX-Y-Q-R 2 3 R4

(I)

wherein R 1

R

2

R

3

R

4 A, E, Q, X and Y are each as described immediately above, which comprises: 1) reacting a compound of the formula:

E

R'-A-N CH-NH 2 R3 R4

(V)

or its salt with a compound of the formula: HO-Qa-R 2

(III)

or its reactive derivative at the carboxy or sulfo group, or 15 a salt' thereof to provide a compound of the formula: R'-A-N CH-NH-Q-R2 R 3 R 4(Ic)

O

II

or its salt, in the above formulas, Qa is or -SO 2 and R R 2

R

3

R

4 A and E are each as described above or 20 2) reacting a compound of the formula: 3

VE

I R1-A-N CH-NH 2 A R R

(V)

P:\OPERUgc\18')44Xclachimns.doc4)3/)3/)3 -8Bor its salt with a compound of the formula: R6-NCO (IV) to provide a compound of the formula:

O

E6 R'-A-N CH-NHCNH-R6 R3 4 (Id) or its salt, in the above formulas, R 6 is phenyl which may be substituted with halogen; or pyridyl, and R 1

R

3

R

4 A and E are each as described above, or 3) reacting a compound of the formula:

E

HN

X-Y-Q-R

2 13 1K4 R R

(VI)

or its salt with a compound of the formula:

R

1 -A-OH (VII) or its reactive derivative at the carboxy or sulfo group, or a salt thereof to provide a compound of the formula:

E

RI-A-N

X-Y-Q-R

2 R3 R 4 R R (I) 9* or its salt, in the above formulas, 1 R 2

R

3 A, E, X, Y and Q are each as described above. R R R A, E, X, Y and Q are each as described above.

In a further aspect of the invention there is provided P:\OPERUgc\ I89)4-4) clains.doc-)3nO)3)3 -8Ca method for therapeutic treatment and/or prevention of amnesia, dementia or schizophrenia which comprises administering an effective amount of a compound as described above in the first aspect of the invention to mammals.

In a further aspect of the invention there is provided a use of a compound of the first aspect of the invention as described above for the manufacture of a medicament for treating and/or preventing amnesia, dementia or schizophrenia in mammals.

C

C

C

C C P 'OPER1gc\19014- cdims dloc-)3/l03 -8D- In the above and subsequent description of the present specification, suitable examples of the various definitions to be included within the scope of the invention are explained in detail in the following.

The term "lower" is intended to mean a group having 1 to 6 carbon atom(s), unless otherwise provided.

The lower moiety in the term "lower alkenyl", "lower alkenyloxy", "lower alkenylamino", "lower alkynyl", "lower alkynyloxy" and "lower alkynylamino" is intended to mean a group having 2 to 6 carbon atoms.

The lower moiety in the terms "cyclo(lower)alkyl", "cyclo(lower)alkyloxy" and "cyclo(lower)alkylamino" is intended to mean a group having 3 to 6 carbon atoms.

Suitable "lower alkyl" and lower alkyl moiety in the terms "substituted-lower alkyl", "ar(lower)alkyl", "halo(lower)alkyl", "lower alkylamino", "lower alkylsilyl", "lower alkylthio" and "lower alkylsulfonyl" may be a straight or branched C 1

-C

6 alkyl such as methyl, ethyl, propyl, isopropyl, butyl, isobutyl, tert-butyl, pentyl, ethylpropyl, hexyl br the like, in which preferable one is methyl.

Suitable "lower alkenyl" and lower alkenyl moiety in the terms "lower alkenyloxy" and "lower alkenylamino" may be a straight or branched C 2

-C

6 alkenyl such as ethenyl, propenyl, butenyl, pentenyl, hexenyl, isopropenyl, butadienyl, pentadienyl, hexadienyl or the like, in which preferable one is ethenyl, propentyl or butadienyl.

Suitable "lower alkynyl" and lower alkynyl moiety in the terms "lower alkynyloxy" and "lower alkynylamino" may be a straight or branched C 2

-C

6 alkynyl such as ethynyl, propargyl, WO 00/42011 PCT/JPO/00017 9 butynyl or the like, in which preferable one is ethynyl.

Suitable "cyclo(lower)alkyl" and cyclo(lower)alkyl moiety in the terms "cyclo(lower)alkyloxy" and "cyclo(lower)alkylamino" may be cyclo(C 3

-C

6 )alkyl such as cyclopropyl, cyclobutyl, cyclopentyl or cyclohexyl, in which preferable one is cyclopropyl.

Suitable "aryl" and aryl or ar moiety in the terms "ar(lower)alkoxy", "aryloxy", "arylamino", "arylsulfonyl", "aroyl" and "ar(lower)alkyl" may be phenyl, naphthyl, phenyl substituted with lower alkyl tolyl, xylyl, mesityl, cumenyl, di(tert-butyl)phenyl, etc.] and the like, in which preferable one is phenyl or tolyl.

Suitable "ar(lower)alkyl" may be benzyl, phenethyl, phenylpropyl, benzhydryl, trityl and the like, in which preferable one is benzyl.

Suitable "lower alkylene" and lower alkylene moiety in the term "lower alkylenedioxy" may be a straight or branched

C

1

-C

6 alkylene such as methylene, ethylene, trimethylene, propylene, tetramethylene, pentamethylene, hexamethylene, ethylethylene or the like, in which preferable one is methylene, ethylene or trimethylene.

Suitable "lower alkoxy" and lower alkoxy moiety in the terms "ar(lower)alkoxy" and "halo(lower)alkoxy" may be a straight or branched C 1

-C

6 alkoxy such as methoxy, ethoxy, propoxy, isopropoxy, methylpropoxy, butoxy, isobutoxy, tertbutoxy, pentyloxy, hexyloxy or the like, in which preferable one is methoxy or tert-butoxy.

Suitable "ar(lower)alkoxy" may be benzyloxy, phenethyloxy, phenylpropoxy, benzhydryloxy, trityloxy and the like.

Suitable "halogen" and halo moiety in the term "halo(lower)alkyl" may be fluorine, chlorine, bromine and iodine, in which preferable one is fluorine, chlorine or iodine.

Suitable "halo(lower)alkyl" may be lower alkyl WO 00/42011 PCT/JPO0/00017 substituted with one or more halogens such as chloromethyl, dichloromethyl, fluoromethyl, difluoromethyl, trifluoromethyl, pentachloroethyl or the like, in which preferable one is trifluoromethyl.

Suitable "halo(lower)alkoxy" may be lower alkoxy substituted with one or more halogens such as chloromethoxy, dichloromethoxy, fluoromethoxy, difluoromethoxy, trifluoromethoxy, pentachloromethoxy or the like, in which preferable one is trifluoromethoxy.

Suitable "lower alkylamino" may be mono or di(lower alkylamino) such as methylamino, ethylamino, porpylamino, isopropylamino, butylamino, tert-butylamino, isobutylamino, pentylamino, hexylamino, dimethylamino, diethylamino, dipropylamino, dibutylamino, diisopropylamino, dipentylamino, dihexylamino, N-methylethylamino or the like, in which preferable one is dimethylamino.

Suitable "lower alkylsilyl" may be mono, di, or tri(lower)alkylsilyl such as trimethylsilyl, dimethylsilyl, triethylsilyl or the like, in which preferable one is trimethylsilyl.

Suitable "lower alkylenedioxy" may be methylenedioxy, ethylenedioxy and the like, in which preferable one is methylenedioxy.

Suitable "heterocyclic group" may be one containing at least one hetero atom selected from nitrogen, sulfur and oxygen atom, and may include saturated or unsaturated, monocyclic or polycyclic heterocyclic group, and preferable heterocyclic group may be N-containing heterocyclic group such as unsaturated 3 to 6-membered heteromonocyclic group containing 1 to 4 nitrogen atoms, for example, pyrrolyl, pyrrolinyl, imidazolyl, pyrazolyl, pyridyl, pyrimidinyl, pyrazinyl, pyridazinyl, triazolyl 4H-1,2,4-triazolyl, 1H-l,2,3-triazolyl, 2H-1,2,3-triazolyl, etc.], tetrazolyl 1H-tetrazolyl, 2H-tetrazolyl, etc.], etc.; saturated 3 to 7-membered heteromonocyclic group containing 1 WO 00/42011 PCT/JP00/00017 11 to 4 nitrogen atoms pyrrolidinyl, imidazolidinyl, piperidyl, piperazinyl, homopiperazinyl, etc.]; unsaturated condensed heterocyclic group containing 1 to nitrogen atoms, for example, indolyl, isoindolyl, indolizinyl, benzimidazolyl, quinolyl, isoquinolyl, imidazopyridyl, indazolyl, benzotriazolyl, tetrazolopyridazinyl tetrazolo[l,5-b]pyridazinyl, etc.], quioxalinyl, etc.; unsaturated 3 to 6-membered heteromonocyclic group containing an oxygen atom, for example, pyranyl, furyl, etc.; saturated 3 to 6-membered heteromonocyclic group containing an oxygen atom, for example, 1H-tetrahydropyranyl, tetrahydrofuranyl, etc.; unsaturated 3 to 6-membered heteromonocyclic group containing 1 to 2 sulfur atoms, for example, thienyl, etc.; unsaturated 3 to 6-membered heteromonocyclic group containing 1 to 2 oxygen atoms and 1 to 3 nitrogen atoms, for example, oxazolyl, isoxazolyl, oxadiazolyl 1,2,4-oxadiazolyl, 1,3,4-oxadiazolyl, 1,2,5-oxadiazolyl, etc.], oxazolinyl [e.g.

2-oxazolinyl, etc.], etc.; saturated 3 to 6-membered heteromonocyclic group containing 1 to 2 oxygen atoms and 1 to 3 nitrogen atoms morpholinyl, etc.]; unsaturated condensed heterocyclic group containing 1 to 2 oxygen atoms and 1 to 3 nitrogen atoms benzofurazanyl, benzoxazolyl, benzoxadiazolyl, etc.]; unsaturated 3 to 6-membered heteromonocyclic group containing 1 to 2 sulfur atoms and 1 to 3 nitrogen atoms, for example, thiazolyl, thiadiazolyl 1,2,4-thiadiazolyl, 1,3,4thiadiazolyl, 1,2,5-thiadiazolyl, etc.], etc.; saturated 3 to 6-membered heteromonocyclic group containing 1 to 2 sulfur atoms and 1 to 3 nitrogen atoms [e.g.

thiazolidinyl, etc.]; unsaturated condensed heterocyclic group containing 1 to 2 sulfur atoms and 1 to 3 nitrogen atoms benzothiazolyl, WO 00/42011 of, m~ mn 12 r wj/IUUII benzothiadiazolyl, etc.]; unsaturated condensed heterocyclic group containing 1 to 2 oxygen atoms benzofuranyl, benzodioxolyl, chromanyl, etc.] and the like.

Said "heterocyclic group" may be substituted with lower alkyl as exemplified above, in which preferable one is thienyl, pyridyl, methylpyridyl, quinolyl, indolyl, quinoxalinyl, benzofuranyl or tetramethylchromanyl, and more preferable one is pyridyl.

Suitable "acyl" may be carboxy; esterified carboxy; carbamoyl substituted with lower alkyl, aryl, ar(lower)alkyl, arylsulfonyl, lower alkylsulfonyl or a heterocyclic group; substituted or unsubstituted arylsulfonyl; lower alkylsulfonyl; cyclo(lower)alkylcarbonyl; lower alkanoyl; substituted or unsubstituted aroyl; a heterocycliccarbonyl and the like.

The esterified carboxy may be substituted or unsubstituted lower alkoxycarbonyl methoxycarbonyl, ethoxycarbonyl, propoxycarbonyl, butoxycarbonyl, tert-butoxycarbonyl, hexyloxycarbonyl, 2-iodoethoxycarbonyl, 2,2,2-trichloroethoxycarbonyl, etc.], substituted or unsubstituted aryloxycarbonyl phenoxycarbonyl, 4-nitrophenoxycarbonyl, 2-naphthyloxycarbonyl, 'etc.], substituted or unsubstituted ar(lower)alkoxycarbonyl [e.g.

benzyloxycarbonyl, phenethyloxycarbonyl, benzhydryloxycarbonyl, 4-nitrobenzyloxycarbonyl, etc.] and the like, in which preferable one is unsubstituted lower alkoxycarbonyl and more preferable one is methoxycarbonyl or tert-butoxycarbonyl.

The carbamoyl substituted with lower alkyl may be methylcarbamoyl, ethylcarbamoyl, propylcarbamoyl, dimethylcarbamoyl, diethylcarbamoyl, N-methyl-Nethylcarbamoyl and the like.

The carbamoyl substituted with aryl may be phenylcarbamoyl, naphthylcarbamoyl, lower alkyl-substituted WO00/42011 PCT/JP00/00017 13 phenylcarbamoyl tolylcarbamoyl, xylylcarbamoyl, etc.] and the like.

The carbamoyl substituted with ar(lower)alkyl may be benzylcarbamoyl, phenethylcarbamoyl, phenylpropylcarbamoyl and the like, in which preferable one is benzylcarbamoyl.

The carbamoyl substituted with arylsulfonyl may be phenylsulfonylcarbamoyl, tolylsulfonylcarbamoyl and the like.

The carbamoyl substituted with lower alkylsulfonyl may be methylsulfonylcarbamoyl, ethylsulfonylcarbamoyl and the like.

The carbamoyl substituted with a heterocyclic group may be one substituted with a heterocyclic group as mentioned above.

The lower alkanoyl may be formyl, acetyl, propionyl, butyryl, isobutyryl, valeryl, isovaleryl, pivaloyl, hexanoyl and the.like, in which preferable one is acetyl or pivaloyl.

The substituted or unsubstituted aroyl may be benzoyl, naphthoyl, toluoyl, di(tert-butyl)benzoyl, halo(lower)alkoxybenzoyl trifluoromethoxybenzoyl, etc.] and the like, in which preferable one is benzoyl or trifluoromethoxybenzoyl.

The substituted or unsubstituted arylsulfonyl may be phenylsulfonyl, tolylsulfonyl, halophenylsulfonyl [e.g.

fluorophenylsulfonyl, etc.] and the like, in which preferable one is fluorophenylsulfonyl.

The lower alkylsulfonyl may be methylsulfonyl, ethylsulfonyl and the like, in which preferable one is methylsulfonyl.

The cyclo(lower)alkylcarbonyl may be cyclo.(C 3

-C

6 alkylcarbonyl such as cyclopropylcarbonyl, cyclobutylcarbonyl, cyclopentylcarbonyl or cyclohexylcarbonyl, in which preferable one is cyclopropylcarbonyl.

The heterocyclic moiety in the term "a heterocycliccarbonyl" may be one mentioned above as a WO00/42011 PCT/JPOO/00017 14 heterocyclic group.

Suitable "acid residue" may be halogen fluoro, chloro, bromo, iodo], arenesulfonyloxy [e.g.

benzenesulfonyloxy, tosyloxy, etc.], alkanesulfonyloxy [e.g.

mesyloxy, ethanesulfonyloxy, etc.], and the like, in which preferable one is halogen.

Suitable "N-protective group" may be common N-protective group such as substituted or unsubstituted lower alkanoyl formyl, acetyl, propionyl, trifluoroacetyl, etc.], lower alkoxycarbonyl tert-butoxycarbonyl, tert-amyloxycarbonyl, etc.], substituted or unsubstituted aralkyloxycarbonyl benzyloxycarbonyl, p-nitrobenzyloxycarbonyl, etc.], 9-fluorenylmethoxycarbonyl, substituted or unsubstituted arenesulfonyl [e.g.

benzenesulfonyl, tosyl, etc.], nitrophenylsulfenyl, aralkyl trityl, benzyl, etc.] or the like, in which preferable one is lower alkoxycarbonyl and more preferable one is tertbutoxycarbonyl.

Suitable "cyclic hydrocarbon" may be a saturated or unsaturated cyclic hydrocarbon such as cyclopentane, cyclohexane, benzene, naphthalene, indan, indene or the like.

Suitable "substituted-lower alkyl" may be lower alkyl substituted with halogen, aryl, acyl, lower alkoxy, aryloxy and the like, in which preferable one is benzyl.

Suitable "heterocyclic ring" may be one which is a heterocyclic group, as mentioned above, added by hydrogen.

Preferred "acyl" for R 1 may be lower alkanoyl; lower alkoxycarbonyl; aroyl optionally substituted with halo(lower)alkoxy; arylsulfonyl optionally substituted with halogen; lower alkylsulfonyl; or cyclo(lower)alkylcarbonyl, in which more preferable one is acetyl, pivaloyl, methoxycarbonyl, tert-butoxycarbonyl, benzoyl, trifluoromethoxybenzoyl, fluorophenylsulfonyl, methylsulfonyl WO 00/42011 PCT/JP00/00017 or cyclopropylcarbonyl.

Preferred "suitable substituent" as the substituent of lower alkyl, lower alkoxy, lower alkylamino, lower alkenyl, lower alkenyloxy, lower alkenylamino, lower alkynyl, lower alkynyloxy, lower alkynylamino, cyclo(lower)alkyl, cyclo(lower)alkyloxy, cyclo(lower)alkylamine, aryl, aryloxy, arylamino, a heterocyclic group or amino substituted a heterocyclic group for R 2 may be halo(lower)alkyl, halo(lower)alkoxy, lower alkenyl, lower alkynyl, lower alkylamino, acylamino, acyl, lower alkylsilyl, lower alkoxy, aryl, lower alkylenedioxy, acyloxy, hydroxy, nitro, amino, cyano, halogen, aryloxy, lower alkylthio and the like.

Preferred "aryl which may be substituted with suitable substituent(s)" for R 2 may be aryl optionally substituted with halogen, in which more preferable one is fluorophenyl.

Preferred "arylamino which may be substituted with suitable substituent(s)" for R 2 may be arylamino optionally substituted with halogen, in which preferable one is phenylamino or fluorophenylamino.

Preferred "aryloxy which may be substituted with suitable substituent(s)" for R 2 may be aryloxy optionally substituted with halogen, in which preferable one is fluorophenoxy.

Preferred "lower alkylene" for Y may be methylene.

Preferred "lower alkyl" for R 5 in Y may be methyl.

Preferred "N-protective group" for R 5 in Y may be tertbutoxycarbonyl.

Preferred "suitable substituent" as the substituent of lower alkylene for E may be oxo, lower alkyl, hydroxy(lower)alkyl or acyl, in which more preferable one is oxo, dioxo, methyl, dimethyl, hydroxymethyl, or benzylcarbamoyl.

Preferred "lower alkylne" for E may be methylene, ethylene or trimethylene, and more preferable one is ethylene.

Preferred "lower alkyl" for R 3 and R 4 may be methyl.

WO00/42011 PCT/JPO/00017 16 Preferred "lower alkylene which R 3 and R 4 are taken together to form" may be ethylene or trimethylene.

Preferred "a cyclic hydrocarbon with which lower alkylene is condensed" may be benzene.

Preferred compound is one having lower alkanoyl, lower alkoxycarbonyl, aroyl, aroyl substituted with halo(lower)alkoxy, lower alkylsulfonyl, arylsulfonyl, arylsulfonyl substituted with halogen or cyclo(lower)alkylcarbonyl for R 1 aryl, aryloxy or arylamino, each aryl of which may be substituted with halogen; pyridyl; or pyridylamino for R 2 a single bond for H O A, ethylene for E, CH for X, for Y, for Q, and ethylene for R 3 and R 4 to be taken together to form, or lower alkanoyl, lower alkoxycarbonyl, aroyl, aroyl substituted with halo(lower)alkoxy, lower alkylsulfonyl, arylsulfonyl, arylsulfonyl substituted with halogen or cyclo(lower)alkylcarbonyl for R 1 aryl, aryloxy or arylamino, each aryl of which may be substituted with halogen; pyridyl; or pyridylamino for R 2 a single bond for

O

A, ethylene for E, N for X, a single bond for Y, for Q, and ethylene for R 3 and R 4 to be taken together to form.

Suitable pharmaceutically acceptable salts of the object compound are conventional non-toxic salts and include acid addition salt such as an inorganic acid addition salt hydrochloride, hydrobromide, sulfate, phosphate, etc.], an organic acid addition salt formate, acetate, trifluoroacetate, maleate, tartrate, methanesulfonate, benzenesulfonate, toluenesulfonate, etc.], a salt with an amino acid aspartic acid salt, glutamic acid salt, etc.], a metal salt such as an alkali metal salt sodium salt, potassium salt, etc.] and alkaline earth metal salt calcium salt, magnesium salt, etc.] and the like.

WO 00/42011 PCT/JPn00/0nnl0 7 17 The processes for preparing the object compound are explained in detail in the following.

Process 1 The compound [Ia] or its salt can be prepared by reacting a compound [II] or its salt with a compound [III] or its reactive derivative at the carboxy or sulfo group, or a salt thereof.

Suitable salts of the compounds [Ia] and [II] may be the same as those exemplified for the compound Suitable salts of the compound [III] and its reactive derivative at the carboxy or sulfo group may be metal salt or alkaline earth metal salt as exemplified for the compound Suitable reactive derivative at the carboxy or sulfo group or the compound [III] may include an ester, an acid halide, an acid anhydride and the like. The suitable examples of the reactive derivatives may be an acid halide acid chloride, acid bromide, etc.]; a symmetrical acid anhydride; a mixed acid anhydride with an acid such as aliphatic carboxylic acid acetic acid, pivalic acid, etc.], substituted phosphoric acid [e.g.

dialkylphosphoric acid, diphenylphosphoric acid, etc.]; an ester such as substituted or unsubstituted lower alkyl ester methyl ester, ethyl ester, propyl ester, hexyl ester, trichloromethyl ester, etc.], substituted or unsubstituted ar(lower)alkyl ester benzyl ester, benzhydryl ester, p-chlorobenzyl ester, etc.], substituted or unsubstituted aryl ester phenyl ester, tolyl ester, 4-nitrophenyl ester, 2,4-dinitrophenyl ester, pentachlorophenyl ester, naphthyl ester, etc.], or an ester with N,N-dimethylhydroxylamine, N-hydroxysuccinimide, N-hydroxyphthalimide or 1-hydroxybenzotriazole, l-hydroxy-6chloro-1H-benzotriazole, or the like. These reactive derivatives can be optionally selected according to the kind of the compound [III] to be used.

WO 00/42011 PC'TI ID/nf/nn i1 18 -aI UUIUUUI V The reaction is usually carried out in a conventional solvent such as water, acetone, dioxane, chloroform, methylene chloride, ethylene dichloride, tetrahydrofuran, acetonitrile, ethyl acetate, N,N-dimethylformamide, pyridine or any other organic solvent which does not adversely influence the reaction. Among these solvents, hydrophilic solvent may be used in a mixture with water.

The reaction is also preferably carried out in the presence of a conventional base such as triethylamine, diisopropylethylamine, pyridine, N,N-dimethylaminopyridine, etc., or a mixture thereof.

When the compound [III] is used in a free acid form or its salt form in the reaction, the reaction is preferably carried out in the presence of a conventional condensing agent such as N,N'-dicyclohexylcarbodiimide, N-cyclohexyl-N'-morpholinoethylcarbodiimide, N-ethyl-N'-(3-dimethylaminopropyl)carbodiimide, thionyl chloride, oxalyl chloride, lower alkoxycarbonyl halide [e.g.

ethyl chloroformate, isobutyl chloroformate, etc.], 1-(p-chlorobenzenesulfonyloxy)-6-chloro-lH-benzotriazole, or the like.

The reaction temperature is not critical, and the reaction can be carried out under cooling to heating.

Process 2 The compound [Ib] or its salt can be prepared by reacting a compound [III or its salt with a compound [IV].

Suitable salts of the compounds [Ib] and [II] may be the same as those exemplified for the compound This reaction is usually carried out in a solvent such as dioxane, tetrahydrofuran, benzene, toluene, chloroform, methylene chloride or any other organic solvent which does not adversely influence the reaction.

The reaction temperature is not critical, and the reaction is usually carried out under cooling to warming.

WO 00/42011 PCT/JP00/00017 19 Process 3 The compound [Ic] or its salt can be prepared by reacting a compound or its salt with a compound [III] or its reactive derivative at the carboxy or sulfo group, or a salt thereof.

Suitable salts of the compounds [Ic] and may be the same as those exemplified for the compound Suitable-salts of the compound [III] and its reactive derivative at the carboxy or sulfo group may be metal salt or alkaline earth metal salt as exemplified for the compound This reaction can be carried out in substantially the same manner as Process 1, and therefore the reaction mode and reaction condition solvent, reaction temperature, etc.] of this reaction are to be referred to those as explained in Process 1.

Process 4 The compound [Id] or its salt can be prepared by reacting a compound or its salt with a compound [IV].

Suitable salts of the compounds [Id] and may be the same as those exemplified for the compound This reaction can be carried out in substantially the same manner as Process 2, and therefore the reaction mode and reaction condition solvent, reaction temperature, etc.] of this reaction are to be referred to those explained in Process 2.

Process The compound or its salt can be prepared by reacting a compound [VI] or its salt with a compound [VII] or its reactive derivative at the carboxy or sulfo group, or a salt thereof.

Suitable salt of the compound [VI] may be acid addition salt as exemplified for the compound WO 00/42011 PCT/JPOO/00017 Suitable salts of the compound [VII] and its reactive derivative at the carboxy or sulfo group may be metal salt or alkaline earth metal salt as exemplified for the compound This reaction can be carried out in substantially the same manner as Process 1, and therefore the reaction mode and reaction condition solvent, reaction temperature, etc.] of this reaction are to be referred to those as explained in Process 1.

Process 6 The compound [Ie] or its salt can be prepared by reacting a compound [VIII] or its reactive derivative at the carboxy group or sulfo group, or a salt thereof with a compound [IX] or its salt.

Suitable salts of the compounds [VIII] and its reactive derivative at the carboxy or sulfo group may be the same as those exemplified for the compound Suitable salt of the compound [IX] may be acid addition salt as exemplified for the compound This reaction can be carried out in substantially the same manner as Process 1, and therefore the reaction mode and reaction condition solvent, reaction temperature, etc.] of this reaction are to be referred to those as explained in Process 1.

Process 7 The compound [If] can be prepared by reacting a compound or its salt with a compound [XI].

Suitable salts of the compounds [If] and may be the same as those exemplified for the compound The present reaction is preferably carried out in the presence of base such as an alkali metal lithium, sodium, potassium, etc.], alkaline earth metal calcium, etc.], alkali metal hydride sodium hydride, etc.], alkaline earth metal hydride calcium hydride, etc.], WO 00/42011 PCT/JP00/00017 21 the hydroxide or carbonate or bicarbonate of an alkali metal or an alkaline earth metal potassium bicarbonate, etc.] and the like.

This reaction is usually carried out in a solvent such as N,N-dimethylformamide, diethyl ether, tetrahydrofuran, dioxane, benzene, toluene, acetonitrile or any other solvent which does not adversely influence the reaction.

The reaction temperature is not critical, and the reaction is usually carried out under cooling to heating.

Process 8 The object compound [Ig] of its salt can be prepared by subjecting a compound [If] or its salt to elimination reaction of the N-protective group.

Suitable salts of the compounds [If] and [Ig] may be acid addition salts as exemplified for the compound This reaction is carried out in accordance with a conventional method such as hydrolysis, reduction or the like.

The hydrolysis is preferably carried out in the presence of a base or an acid including Lewis acid.

Suitable base may include an inorganic base and an organic base such as an alkali metal sodium, potassium, etc.], an alkaline earth metal magnesium, calcium, etc.], the hydroxide or carbonate or bicarbonate thereof, hydrazine, alkylamine methylamine, trimethylamine, triethylamine, etc.], picoline, 1,5-diazabicyclo[4.3.0]non- 1,4-diazabicyclol2.2.2]octane, 1,8-diazabicyclo- [5.4.0]undec-7-ene, or the like.

Suitable acid may include an organic acid formic acid, acetic acid, propionic acid, trichloroacetic acid, trifluoroacetic acid, etc.], an inorganic acid [e.g.

hydrochloric acid, hydrobromic acid, sulfuric acid, hydrogen chloride, hydrogen bromide, hydrogen fluoride, etc.] and an acid addition salt compound pyridine hydrochloride, etc.].

WO 00/42011 PCT/IT/Dn n InnlI 22 ~fU J I The elimination using trihaloacetic acid [e.g.

trichloroacetic acid, trifluoroacetic acid, etc.] or the like is preferably carried out in the presence of cation trapping agents anisole, phenol, etc.].

The reaction is usually carried out in a solvent such as water, an alcohol methanol, ethanol, etc.], methylene chloride, chloroform, tetrachloromethane, dioxane, tetrahydrofuran, a mixture thereof or any other solvent which does not adversely influence the reaction. A liquid base or acid can be also used as the solvent. The reaction temperature is not critical and the reaction is usually carried out under cooling to heating.

The reduction method applicable for the elimination reaction may include chemical reduction and catalytic reduction.

Suitable reducing agents to be used in chemical reduction are a combination of metal tin, zinc, iron, etc.] or metallic compound chromium chloride, chromium acetate, etc.] and an organic or inorganic acid formic acid, acetic acid, propionic acid, trifluoroacetic acid, p-toluenesulfonic acid, hydrochloric acid, hydrobromic acid, etc.].

Suitable catalysts to be used in catalytic reduction are conventional ones such as platinum catalysts platinum plate, spongy platinum, platinum black, colloidal platinum, platinum oxide, platinum wire, etc.], palladium catalysts spongy palladium, palladium black, palladium oxide, palladium on carbon, colloidal palladium, palladium on barium sulfate, palladium on barium carbonate, etc.], nickel catalysts reduced nickel, nickel oxide, Raney nickel, etc.], cobalt catalysts reduced cobalt, Raney cobalt, etc.], iron catalysts reduced iron, Raney iron, etc.], copper catalysts reduced copper, Raney copper, Ullman copper, etc.] and the like.

In case that the N-protective group is benzyl, the WO 00/42011 PCT/JPOO/00017 23 reduction is preferably carried out in the presence of a combination of palladium catalysts palladium black, palladium on carbon, etc.] and formic acid or its salt [e.g.

ammonium formate, etc.].

The reduction is usually carried out in a conventional solvent which does not adversely influence the reaction such as water, methanol, ethanol, propanol, N,N-dimethylformamide, or a mixture thereof. Additionally, in case that the abovementioned acids to be used in chemical reduction are in liquid, they can also be used as a solvent. Further, a suitable solvent to be used in catalytic reduction may be the above-mentioned solvent, and other conventional solvent such as diethyl ether, dioxane, tetrahydrofuran, etc. or a mixture thereof.

The reaction temperature of this reduction is not critical and the reaction is usually carried out under cooling to heating.

Process 9 The compound [Ii] or its salt can be prepared by reacting a compound [Ih] or its salt with a compound [XII].

Suitable salts of the compounds [Ih] and [Ii] may be the same as those exemplified for the compound This reaction can be carried out in substantially the same manner as Process 7, and therefore the reaction mode and reaction condition solvent, reaction temperature, etc.] of this reaction are to be referred to those explained in Process 7.

Process The compound [Ij] or its salt can be prepared by reacting a compound [II] or its salt with a compound [XIII].

Suitable salts of the compounds [Ij] and [II] may be the same as those exemplified for the compound This reaction can be carried out in substantially the WO 00/42011 PCTIJPO0/O0017 24 same manner as Process 7, and therefore the reaction mode and reaction condition solvent, reaction temperature, etc.] of this reaction are to be referred to those explained in Rrocess 7.

The compounds obtained by the above processes can be isolated and purified by a conventional method such as pulverization, recrystallization, column chromatography, reprecipitation, or the like.

It is to be noted that the compound and the other compounds may include one or more stereoisomer(s) such as optical isomer(s) or geometrical isomer(s) due to asymmetric carbon atom(s) and double bond(s), and all of such isomers and mixture thereof are included within the scope of this invention.

Additionally, it is to be noted that any solvate [e.g.

enclosure compound hydrate, etc.)] of the compound [I] or a pharmaceutically acceptable salt thereof is also included within the scope of this invention.

The object compound and pharmaceutically acceptable salts thereof possess strong potentiation of the cholinergic activity, and are useful for the treatment and/or prevention of disorders in the central nervous system for mammals, and more particularly of amnesia, dementia senile dementia, Alzheimer's dementia, dementia associated with various diseases such as cerebral vascular dementia, cerebral posttraumatic dementia, dementia due to brain tumor, dementia due to chronic subdural hematoma, dementia due to normal pressure hydrocephalus, post-meningitis dementia, Parkinson's disease type dementia, etc.) and the like. Additionally, the object compound is expected to be useful as therapeutical and/or preventive agents for schizophrenia, depression, stroke, head injury, nicotine withdrawal, spinal cord injury, anxiety, pollakiuria, incontinence of urine, myotonic dystrophy, attention deficit hyperactivity disorder, excessive daytime sleepiness (narcolepsy), Parkinson's disease or autism.

WO 00/42011 PCT/JP00/00017 In order to illustrate the usefulness of the object compound the pharmacological data of the compound is shown in the following.

Test Penile erection in rat (This test was carried out according to a similar manner to that described in Jpn. J. Pharmacol., Vol. 64, 147-153 (1994)) Method Male Fischer 344 rats at the age of 8 weeks were used. All rats were handled 3 minutes a day for three successive days before the tests. The rats were tested in groups of seven and various doses of the test compound were given in semi-randomized order. The test compounds were suspended in 0.5% methyl-cellulose immediately before use, and given intraperitoneally in a volume of 1 ml/kg just before the start of test. Immediately after injection, each rat was placed in a perspex box (25x25x35 cm) and its behavior was observed for 60 minutes, during which time the number of penile erections was counted. A mirror was situated behind each box to facilate of the rat. Data was expressed as a mean number.

(ii) Test Result Test Compound Dose Penile Erection (Example No.) (mg/kg) (number/hr) 2 1 1.14 19 0.32 0.75 It is clear that the compound having the above-mentioned WO 00/42011 PCT/JPOO00017 26 activity ameliorates the memory deficits amnesia, dementia, etc.) from the description in the Journal of Pharmacology and Experimental Therapeutics, Vo. 279, No. 3, 1157-1173 (1996). Further, it is expected that the compound having the above-mentioned activity is useful as therapeutical and/or preventive agent for aforesaid diseases from some patent applications PCT International Publication No. WO 98/27930, etc.).

For therapeutic purpose, the compound and a pharmaceutically acceptable salt thereof of the present invention can be used in a form of pharmaceutical preparation containing one of said compounds, as an active ingredient, in admixture with a pharmaceutically acceptable carrier such as an organic or inorganic solid, semi-solid or liquid excipient suitable for oral or parenteral administration. The pharmaceutical preparations may be capsules, tablets, dragees, granules, suppositories, solution, suspension, emulsion, or the like. If desired, there may be included in these preparations, auxiliary substances, stabilizing agents, wetting or emulsifying agents, buffers and other commonly used additives.

While the dosage of the compound will vary depending upon the age and condition of the patient, an average single dose of about 0.1 mg, 1 mg, 10 mg, 50 mg, 100 mg, 250 mg, 500 mg and 1000 mg of the compound may be effective for treating the above-mentioned diseases. In general, amounts between 0.1 mg/body and about 1,000 mg/body may be administered per day.

The following Preparations and Examples are given for the purpose of illustrating this invention.

Preparation 1 To a solution of l-benzyl-4-aminopiperidine (50 g) in water (360 ml) was added a solution of di-tert-butyl WO 00/42011 PCT/JP00/00017 27 dicarbonate (61 g) in acetone (360 ml) dropwise under cooling on an ice-water bath. After stirring for 2.5 hours, a precipitate was collected on a filter, washed with water, and dried. The crude product was poured into a mixture of diisopropyl ether (200 ml) and n-hexane (200 ml) and the mixture was stirred. After filtration, O-tert-butyl N-(1benzylpiperidin-4-yl)carbamate (66.9 g) was obtained.

NMR (DMSO-d 6 1.2-1.5 (2H, 1.37 (9H, 1.66 (2H, br d, J=9.9Hz), 1.91 (2H, br t, J=10.7Hz), 2.73 (2H, distorted d, J=11.8Hz), 3.2 (1H, 3.41 (2H, 6.75 (1H, d, J=7.8Hz), 7.1-7.4 (SH, m) MASS (APCI)(m/z): 291 Preparation 2 To a mixture of 0-tert-butyl N-(l-benzylpiperidin-4yl)carbamate. (45 g) and 10% palladium on carbon (50% wet, 9 g) in methanol (1 1) was bubbled hydrogen gas under stirring at ambient temperature. The catalyst was removed by glass filter and the solvent was removed under reduced pressure.

After rinse with diisopropyl ether, O-tert-butyl N-(piperidin-4-yl)carbamate (28.35 g) was obtained. The washed solvent was removed under reduced pressure, and the residue was rinsed with diisopropyl ether. The second fraction of O-tert-butyl N-(piperidin-4-yl)carbamate (344 mg) was obtained.

NMR (DMSO-d 6 1.18 (2H, ddd, J=3.8, 11.8, 11.8Hz), 1.37 (9H, 1.62 (2H, distorted d, J=10.8Hz), 1.85 (1H, 2.38 (2H, dt, J=2.2, 12.0Hz), 2.86 (2H, distorted d, J=12.3Hz), 3.2 (1H, 6.72 (1H, br d) MASS (APCI)(m/z): 201 Preparation 3 To a suspension of O-tert-butyl N-(piperidin-4yl)carbamate (4.0 g) in dichloromethane (40 ml) were added WO 00/42011 PCT/JP00/00017 28 pyridine (1.94 ml), dichloromethane (40 ml), acetic anhydride (20.8 ml) and then N,N-dimethylaminopyridine (0.1 g) at ambient temperature. After stirring for 3 hours, the mixture was washed with 0.1N hydrochloric acid, water, and brine.

After drying with magnesium sulfate, the solvents were removed under reduced pressure. After rinse with diisopropyl ether, O-tert-butyl N-(l-acetylpiperidin-4-yl)carbamate (4.01 g) was obtained.

NMR (DMSO-d 6 1.23 (2H, 1.38 (9H, 1.70 (2H, distorted t, J=11.4Hz), 1.97 (3H, 2.64 (1H, br t, J=11.lHz), 3.04 (1H, dt, J=2.8, 11.5Hz), 3.42 (1H, 3.72 (1H, br d, J=15.0Hz), 4.19 (1H, br d, J=13.1Hz), 6.86 (1H, d, MASS (APCI) 243 Preparation 4 To a solution of O-tert-butyl N-(l-acetylpiperidin-4yl)carbamate (2.42 g) in dichloromethane (24 ml) was added 4N hydrogen chloride in dioxane (24 ml). The solvents were removed under reduced pressure. After rinse with diisopropyl ether, l-acetyl-4-aminopiperidine hydrochloride (2.02 g) was obtained.

NMR (DMSO-d 6 1.41 (2H, 1.93 (2H, distorted 2.00 (3H, 2.60 (1H, br t, J=10.4Hz), 3.06 (1H, br t, J=11.3Hz), 3.12 (1H, 3.84 (1H, br d, J=14.0Hz), 4.34 (1H, br d, J=13.0Hz), 8.32 (3H, br s) MASS (APCI) 143 Preparation To a solution of phenyl chloroformate, (5.64 g) in dichloromethane (70 ml) was added a solution of 4-aminopyridine (2.84 g) and triethylamine (5.02 ml) in dichloromethane (100 ml) dropwise under cooling on an icewater bath. After stirring for 1 hour, the solvents were WO 00/42011 PCT/JPO/00017 29 removed under reduced pressure. A residue was diluted with dichloromethane (200 ml) and water (200 ml). An organic phase was separated and washed with water and brine. After drying with magnesium sulfate, the solvents were removed under reduced pressure. The reaction mixture was diluted with diisopropyl ether and the precipitates were filtered.

After rinse with diethyl ether, O-phenyl N-(4pyridyl)carbamate (5.07 g) was obtained.

NMR (CDC13, 7.17 (2H, 7.27 (1H, 7.3-7.5 (4H, 8.50 (2H, dd, J=1.4, 5.0Hz), 8.06 (1H, s) MASS (APCI)(m/z): 215 Preparation 6 A solution of sulfuryl chloride (3.55 ml) in chloroform (45 ml) was added a solution of 1-acetylpiperazine (5.66 mg) and triethylamine (6.16 ml) in chloroform (15 ml) dropwise under cooling on an ice-water bath. After stirring for 6 hours, a precipitate was collected by filtration. After drying over sodium hydroxide, l-acetylpiperazine-4-sulfonyl chloride (2.43 g) was obtained.

NMR (CDC1 3 2.15 (3H, 3.35 (4H, 3.69 (2H, t, J=5.1Hz), 3.83 (2H, br s) MASS (APCI) 227 Preparation 7 To a solution of l-benzyl-4-aminopiperidine (1.13 g) in dichloromethane (10 ml) were added a solution of 4fluorobenzoyl chloride (0.99 g) in dichloromethane (1 ml) and diisopropylethylamine (1.09 ml) under cooling on an ice-water bath. The mixture was warmed to ambient temperature slowly under stirring. The mixture was diluted with dichloromethane and washed with water, saturated aqueous sodium hydrogen carbonate, water, and brine. After drying with magnesium sulfate, the solvents were removed under reduced pressure.

A residue was purified by column chromatography (silica gel WO 00/42011 PCT/JPOO/0001 7 100 ml, dichloromethane:rnethanol 15:1) After rinse with diisopropyl ether n-hexane N-(1-benzylpiperidin-4yl)-4-fluorobenzamide (1.31 g) was obtained.

NMR (DMSO-d 6 8) 1.4-1.7 (2H, in), 1. 7-1. 9 (2H, in), 2. 01 (2H, br t, J=10. 7Hz) 2. 81 (2H, br d, J=1l.6Hz), 3.46 (2H, 3.73 (1H, in), 7.2-7.4 (7H, mn), 7. 90 dd, J=5. 6, 8. 9Hz) 8.26 (1H, br d, J=7.7Hz) MASS (APCI) (mhz) 313 Preparation 8 The following compound was obtained by using 4-amino-ibenzylpiperidine as a starting compound according to a similar manner to that of Example 2.

N- (I1-Benzylpiperidin- 4-yl) (4 fluorophenyl) urea 2.0-2.2 (2H, in), 2.65-2.8 (2H, in), 3.4-3.6 (3H, mn), 6.07 (1H, d, J=7.6Hz), 7.05 (2H, t, J=9Hz), 7.2- 7.45 in), 8.35 (1H, s) MASS (APCI)(in/z) 328 Preparation 9 To a solution of N-(1-benzylpiperidin-4-yl)-N'-(4fluorophenyl)urea (3.0 g) in a mixture of methanol *(15 ml) and tetrahydrofuran (15 ml) was added palladium on carbon w/w, 50% wet, 0.6 and the mixture was hydrogenated under atmospheric pressure of hydrogen for 8 hours. The catalyst was filtered off, and the solvents were evaporated under reduced pressure to give a residue, which was triturated with diisopropyl ether to give 1N-(piperidin-4-yl)- N'-(4-fluorophenyl)urea (1.97 g).

NMR (DMSO-d 6 1.1-1.4 (2H, in), 1.65-1.85 (2H, in), 2.3-2.65 (2H, in), 2.8-3.0 (2H, in), 3.3-3.7 (1H, in), 6.08 (1H, d, J=8Rz), 7.04 (2H, t, J=9Hz), 7.25-7.5 WO 00/42011 PCT/JPOO/00017 31 NMR (DMSO-d 6 1.1-1.4 (2H, 1.65-1.85 (2H, m), 2.3-2.65 (2H, 2.8-3.0 (2H, 3.3-3.7 (1H, m), 6.08 (1H, d, J=8Hz), 7.04 (2H, t, J=9Hz), 7.25-7.5 (2H, 8.33 (1H, s) MASS (APCI)(m/z): 238 Preparation A mixture of N-(l-benzylpiperidin-4-yl)-4fluorobenzamide (937 mg) and 10% palladium on carbon wet, 0.2 g) in methanol (20 ml) was stirred under hydrogen atmosphere for 7.5 hours at ambient temperature. The catalyst was removed by glass filter and the solvent was removed under reduced pressure. After rinse with diisopropyl ether, N-(piperidin-4-yl)-4-fluorobenzamide (653 mg) was obtained.

NMR (DMSO-d 6 1.40 (2H, ddd, J=4.0, 11.9, 23.8Hz), 1.72 (2H, br d, J=9.5Hz), 2.3-2.7 (2H, 2.8-3.2 (2H, 3.80 (1H, 7.27 (2H, t, J=8.9Hz), 7.92 (2H, dd, J=5.6, 8.9Hz), 8.26 (1H, d, J=7.7Hz) MASS (APCI)(m/z): 223 Example 1 To a solution of O-phenyl N-(4-pyridyl)carbamate (446 mg) in 1,2-dichloroethane (5 ml) was added a suspension of 1-acetylpiperazine (1.12 g) in 1,2-dichloroethane (20 ml) at ambient temperature. The mixture was heated at 60°C with stirring for 9 hours. The mixture was cooled to ambient temperature, and diluted with dichloromethane and water.

The aqueous phase was separated and adjusted to pH 11.5 with sodium hydroxide solution. Excess sodium chloride was added to the aqueous solution. The mixture was extracted with a mixture of dichloromethane and methanol (about 10:1) and the organic phase was washed with brine. After drying with magnesium sulfate, the solvents were removed under reduced pressure. A residue was purified by column chromatography WO 00/42011 PCT/JPOO/0001 7 32 (silica gel 100 ml, dichloromethane: methanol: aqueous ammonia 10:1:0.1). After rinse with diisopropyl ether, 1-acetyl- 4-(4-pyridylaminocarbonyl)piperazine (398 mg) was obtained.

NMR (DMSO-1 6 2.03 (3H, 3.3-3.6 in), 7.47 (2H, dd, J=1.5, 4.8Hz), 8.31. (2H, dd, 4.8Hz), 9.01 (1H, s) MASS (APCI) :271 Example 2 To a stirred solution of 1-acetylpiperazine (0.648 g) in tetrahydrofuran (10 ml) was added 4-f luorophenyl isocyanate (0.574 g) at ambient temperature. After stirring at ambient temperature for 1 hour, the solvent was removed by evaporation under reduced pressure, and the residue was tritu *rated with diisopropyl ether to give l-acetyl-4-(4fluorophenylcarbamoyl)piperazine (1.25 g).

NMR (DMSO-d 6 2.03 O3H, 3.3-3.6 (8H, in), 7.07 (2H, t, J=9Hz), 7.46 (2H, dd, J=5, 9Hz), 8.61 (1H, s) MASS (APCI) (mlz) 266 Exampe 3 The following compound was obtained by using I-tertbutoxycarbonylpiperazine as a starting compound according to a similar manner to that of Example 2.

1-tert-Butoxycarbonyl-4- (4-f luorophenylcarbamoyl) piperazine NMR (DMS0-l 6 1.42 (9H, 3.25-3. 5 (8H, in), 7.07 (2H, t, J=9Hz) 7. 45 (2H, dcl, J=5, 9Hz) 8. 60 (1H, s) MASS (LD) 346.2 Exmple 4 To a solution of pyridine-4-carboxylic acid (1.0 g) and WO 00/42011 PCT/JPOO/00017 33 triethylamine (1.2 ml) in toluene (20 ml) was added diphenylphosphoryl azide (1.75 ml) at ambient temperature.

The resulting mixture was heated to reflux for 30 minutes and cooled to 0°C. To the mixture was added 1-tertbutoxycarbonylpiperazine (1.51 and the mixture was allowed to heat to 90 0 C for 1 hour. After cooling to ambient temperature, the reaction mixture was taken up into ethyl acetate, washed in turn with water and brine, dried over magnesium sulfate, and evaporated under reduced pressure.

The residue was chromatographed on silica gel (150 ml) eluting with 0-7% methanol in dichloromethane. Trituration with a mixture of diisopropyl ether and ethanol gave 1-tertbutoxycarbonyl-4-(pyridin-4-ylcarbamoyl)piperazine (0.66 g) NMR (DMSO-d 6 1.42 (9H, 3.25-3.5 (8H, 7.46 (2H, d, J=1.5, 5Hz), 8.30 (2H, d, J=1.5, 9.00 (1H, s) MASS (LD) 307.2 Example To a suspension of l-acetyl-4-aminopiperidine hydrochloride (0.4 g) in dichloromethane (5 ml) were added in turn pyridine (0.54 ml) and 4-fluorophenyl chloroformate (0.29 ml) at The mixture was allowed to warm to ambient temperature and stirred for 1 hour, which was taken up into a mixture of water and ethyl acetate. The separated organic layer was washed in turn with hydrochloric acid (lN), aqueous sodium hydrogen carbonate, and brine, and dried over magnesium sulfate. Evaporation under reduced pressure gave a residue, which was triturated with diisopropyl ether to give l-acetyl-4- (4-fluorophenoxycarbonylamino) piperidine (347 mg).

NMR (DMSO-d 6 1.15-1.55 (2H, 1.7-1.95 (2H, m), 2.00 (3H, 2.65-2.85 (1H, 3.0-3.25 (1H, m), 3.5-3.7 (1H, 3.7-3.9 (1H, 4.15-4.3 (1H, m), 7.05-7.3 (4H, 7.86 (1H, d, J=8Hz) WO00/42011 PCT/JP0/00017 34 MASS (APCI) 281 Example 6 To a suspension of l-acetyl-4-aminopiperidine hydrochloride (715 mg) in dichloromethane (7 ml) were added diisopropylethylamine (1.83 ml) and a solution of 4-fluorobenzoyl chloride (0.83 mg) in dichloromethane (2 ml) at ambient temperature. After stirring for 6.5 hours, the reaction mixture was diluted with dichloromethane and washed with water, saturated aqueous sodium hydrogen carbonate, and brine. After drying with magnesium sulfate, the solvents were removed under reduced pressure. A residue was purified by column chromatography (silica gel 50 ml, dichloromethane:methanol 50:1 to 10:1). After rinse with diisopropyl ether, N-(l-acetylpiperidin-4-yl)-4fluorobenzamide (738 mg) was obtained.

NMR (DMSO-d 6 1.40 (2H, 1.81 (2H, distorted t, J=12.4Hz), 2.01 (3H, 2.68 (1H, br t, J=11.4Hz), 3.13 (1H, br t, J=11.6Hz), 3.83 (1H, br t, J=13.9Hz), 4.01 (1H, 4.33 (1H, br d, J=13.7Hz), 7.29 (2H, t, J=8.9Hz), 7.92 (2H, dd, J=5.5, 8.8Hz), 8.31 (1H, d, J=7.7Hz) MASS (APCI)(m/z): 265 Example 7 To a suspension of l-acetyl-4-aminopiperidine hydrochloride (536 mg) in dichloromethane (5 ml) were added isonicotinoyl chloride hydrochloride (534 mg) and diisopropylethylamine (1.05 ml) at ambient temperature.

After stirring for 8 hours, the reaction mixture was poured into water and diluted with dichloromethane. The mixture was adjusted to pH 8.5 with 1N sodium hydroxide solution.

Sodium chloride was added to the mixture and an organic phase was separated. The aqueous phase was extracted with dichloromethane and a combined organic phase was dried over WO 00/42011 PCTIJPOw/rjfj 7 magnesium sulfate. The solvents were removed under reduced pressure. A residue was purified by column chromatography (silica gel 50 ml, dichloromethane:methanol =10:1) After crystallization from diisopropyl ether:n-hexane, N-(l-acetylpiperidin-4--yl)-N-isonicotinamide (477 mg) was obtained.

NMR (DMSO-d 6 1.4 (2H, in), 1.83 (2H, distorted t, J=llHz), 2.01 (3H, 2.69 (1H, br t, J=llHz), 3.14 (1H, br t, J=l2Hz), 3.83 (1H, br d, J=14.lHz), 4.03 (1H, in), 4.33 (1H, br d, J=13.lHz), 7.75 (2H, dd, J=1.7, 4.4Hz), 8.62 (1H, d, J=7.5Hz), 8.72 (2H, dd, J=1.6, 4.4Hz) MASS (APCI) 248 Examnple 8 To a suspension of 1-acetyl-4-aminopiperidine hydrochloride (715 mg) in dichioromethane (7 ml) were added diisopropylethylamine (1.83 ml) and a solution of 4-fluorobenzenesulfonyl chloride (0.83 mg) in dichloromethane (2 ml) at ambient temperature. After stirring for 6.5 hours, the reaction mixture was diluted with dichloromethane and washed with water, saturated aqueous sodium hydrogen carbonate, and brine. After drying with magnesium sulfate, the solvents were removed under reduced pressure. A residue was purified by column chromatography (silica gel 50 ml, dichloromethane:methanol= 50:1 to 20:1). After rinse with diisopropyl ether, N-(lacetylpiperidin-4-yl)-4-fluorobenzenesulfonamide (859 mng) was obtained.

NMR (DMSO-d 6 1.21 (2H, in), 1.54 (2H, mn), 1.94 (3H, 2.66 (1H, br t, J=10.8H7-), 3.02 (1H, dt, J=2.9, 12.0Hz), 3.22 (1H, in), 3.64 (1H, br d, J=14.OHz), 4.05 (1Hi, br di, J=13.2Hz), 7.44 (2H, t, J=8.9Hz), 7.8-8.0 (3H, in) MASS (APCI) 301 WO 00/42011 PCT/JP00/00017 36 Example 9 To a solution of O-phenyl N-(4-pyridyl)carbamate (0.81 g) in chloroform (10 ml) were added l-acetyl-4aminopiperidine hydrochloride (0.68 g) and triethylamine (1.06 ml) at ambient temperature. After stirring for 1 day, the mixture changed to a solution. The solvents were removed under reduced pressure. A residue was purified by column chromatography (silica gel 100 ml, dichloromethane:methanol 10:1 to 5:1, and silica gel 50 ml, dichloromethane:methanol:aqueous ammonia 10:1:0.1). The solvents of desired fractions were removed under reduced pressure. A residue was dissolved with methanol (5 ml) and dichloromethane (5 ml), and 4N hydrogen chloride in dioxane ml) was added to the solution. The solvents were removed under reduced pressure, and the residue was evaporated azeotropically with methanol. After crystallization from diisopropyl ether and n-hexane, N-(l-acetylpiperidin-4-yl)-N'-(4-pyridyl)urea (343 mg) was obtained.

NMR (DMSO-d 6 1.1-1.6 (2H, 1.77 (2H, 2.01 (3H, 2.94 (1H, br t, J=10.4Hz), 3.22 (1H, br t, J=10.1Hz), 3.76 (2H, 4.05 (1H, d, J=13.6Hz), 7.60 (1H, d, J=7.8Hz), 7.83 (2H, d, J=6.8Hz), 8.52 (2H, d, J=7.1Hz), 11.21 (1H, 14.66 (1H, br s) MASS (APCI)(m/z): 263 Example To a suspension of l-acetyl-4-aminopiperidine hydrochloride (536 mg) in dichloromethane (5 ml) were added 4-fluorophenyl isocyanate (375 pl) and diisopropylethylamine (575 pl) at ambient temperature. After stirring for 3 hours, the reaction mixture was diluted with dichloromethane. An organic phase was separated and an aqueous phase was extracted with dichloromethane. A combined organic phase was dried over magnesium sulfate and the solvents were WO 00/42011 PCT/JP00/00017 37 removed under reduced pressure. After crystallization from diisopropyl ether and n-hexane, N-(l-acetylpiperidin-4-yl)- N'-(4-fluorophenyl)urea (448 mg) was obtained.

NMR (DMSO-d 6 1.1-1.5 (2H, 1.80 (2H, distorted t, J=10Hz), 2.00 (3H, 2.77 (1H, br d, J=10.8Hz), 3.14 (1H, br d, J=11.lHz), 3.5-3.9 (2H, 4.16 (1H, br d, J=13.2Hz), 6.15 (1H, d, J=7.6Hz), 7.05 (2H, t, J=8.9Hz), 7.40 (2H, dd, 9.2Hz), 8.37 (1H, s) MASS (APCI)(m/z): 280 Example 11 To a solution of 4-(4-fluorobenzoylamino)piperidine (0.25 g) in dichloromethane (5 ml) were added in turn pyridine (0.14 ml) and methyl chloroformate (87 pl) at 0°C.

The mixture was allowed to warm to ambient temperature and stirred for 1 hour. To the mixture was added N,Ndimethylaminopyridine (0.13 g) and allowed to stir for another 1 hour. The reaction mixture was taken up into a mixture of water and ethyl acetate. The separated organic layer was washed in turn with hydrochloric acid (IN), aqueous sodium hydrogen carbonate, and brine, and dried over magnesium sulfate. Evaporation under reduced pressure gave a residue, which was triturated with diisopropyl ether to give 4-(4-fluorobenzoylamino)-1-methoxycarbonylpiperidine (0.265 g).

NMR (DMSO-d 6 1.3-1.6 (2H, 1.75-1.9 (2H, m), 2.8-3.05 (2H, 3.60 (3H, 3.85-4.1 (2H, m), 7.29 (2H, t, J=9Hz), 7.90 (2H, dd, J=6, 9Hz), 8.30 (1H, d, J=8Hz) MASS (APCI)(m/z): 281 Example 12 To a solution of 4-(4-fluorobenzoylamino)piperidine (0.25 g) in pyridine (5 ml) were added in turn WO 00/42011 PCT/JPOO/00017 38 4-trifluorobenzenesulfonyl chloride (0.219 g) and catalytic amount of N,N-dimethylaminopyridine at OC.

The mixture was allowed to warm to ambient temperature and stirred for 1 hour, which was taken up into a mixture of water and dichloromethane. The separated organic layer was washed in turn with hydrochloric acid aqueous sodium hydrogen carbonate, and brine, and dried over magnesium sulfate. Evaporation under reduced pressure gave a residue, which was triturated with diisopropyl ether to give 4-(4-fluorobenzoylamino) -1-(4-rifluorophenylsulfonyl)piperidine (0.38 g).

NMR (DMSO-d 6 1.45-1.7 (2H, 1.8-1.95 (2H, m), 2.35-2.55 (2H, 3.5-3.85 (3H, 7.28 (2H, t, J=9Hz), 7.50 (2H, t, J=9Hz), 7.75-7.95 (4H, m), 8.31 (1H, d, J=8Hz) MASS (APCI)(m/z): 381 Example 13 To a solution of 4-(4-fluorobenzoylamino)piperidine (0.15 g) in dichloromethane (5 ml) were added in turn pyridine (82 p.1) and 4-trifluoromethoxybenzoyl chloride (106 1p) at 0°C. The mixture was allowed to warm to ambient temperature and stirred for 4 hours, which was taken up into a mixture of water and dichloromethane. The separated organic layer was washed in turn with hydrochloric acid (IN), aqueous sodium hydrogen carbonate, and brine, and dried over magnesium sulfate. Evaporation of the solvent under reduced pressure gave 4-(4-fluorobenzoylamino)-1-(4trifluoromethoxybenzoyl)piperidine (205 mg).

NMR (DMSO-d 6 1.3-1.7 (2H, 1.7-2.0 (2H, m), 2.7-3.4 (2H, 3.4-3.8 (1H, 3.9-4.2 (1H, m), 4.2-4.6 (1H, 7.30 (2H, t, J=9Hz), 7.35-7.6 (4H, 7.91 (2H, dd, J=6, 9Hz), 8.35 (1H, d, J=8Hz) MASS 433.2 WO 00/42011 PCT/JP00/00017 39 Example 14 To a solution of 4-(4-fluorobenzoylamino)piperidine (0.15 g) in dichloromethane (5 ml) were added in turn pyridine (0.14 ml) and methanesulfonyl chloride (96 at 0 C. The mixture was allowed to warm to ambient temperature and stirred for 1 hour. To the mixture was added N,Ndimethylaminopyridine (0.13 g) and allowed to stir for another 1 hour. The reaction mixture was taken up into a mixture of water and dichloromethane. The separated organic layer was washed in turn with hydrochloric acid (IN), aqueous sodium hydrogen carbonate, and brine, and dried over magnesium sulfate. Evaporation under reduced pressure gave a residue, which was triturated with diisopropyl ether to give 4-(4-fluorobenzoylamino)-1-methylsulfonylpiperidine (0.30 g).

NMR (DMSO-d 6 1.45-1.7 (2H, 1.8-2.05 (2H, m), 2.7-2.95 (2H, 2.88 (3H, 3.5-3.65 (2H, m), 3.8-4.05 (1H, 7.30 (2H, t, J=9Hz), 7.91 (2H, dd, J=6, 9Hz), 8.36 (1H, d, J=8Hz) MASS (APCI)(m/z): 301 Example To a solution of N-(piperidin-4-yl)-N'-(4fluorophenyl)urea (0.3 g) in tetrahydrofuran (4 ml) were added in turn pyridine (0.28 ml), methyl chloroformate (98 p1) and catalytic amount of N,N-dimethylaminopyridine at 0°C. The mixture was allowed to warm to ambient temperature and stirred for 2 hours. The reaction mixture was taken up into a mixture of water and ethyl acetate. The separated organic layer was washed in turn with hydrochloric acid (IN), aqueous sodium hydrogen carbonate, and brine, and dried over magnesium sulfate. Evaporation under reduced pressure gave a residue, which was triturated with diisopropyl ether to give N-(l-methoxycarbonylpiperidin-4-yl)-N'-(4fluorophenyl)urea (0.312 g).

WO 00/42011 PCT/JPO0/00017 NMR. (DMSO-d 6 1.1-1.4 (2H, 1.7-1.9 (2H, m), 2.8-3.1 (2H, 3.5-3.75 (1H, 3.59 (3H, s), 3.75-3.95 (2H, 6.15 (1H, d, J=7.6Hz), 7.05 (2H, t, J=9Hz), 7.37 (2H, dd, J=5, 9Hz), 8.37 (1H, s) MASS (APCI)(m/z): 296 Example 16 To a solution of N-(piperidin-4-yl)-N'-(4fluorophenyl)urea (0.3 g) in tetrahydrofuran (4 ml) were added in turn N,N-dimethylaminopyridine (0.23 g) and 4-fluorobenzenesulfonyl chloride (0.25 g) at 0 0 C. The mixture was allowed to warm to ambient temperature and stirred for 1 hour. The reaction mixture was taken up into a mixture of water and dichloromethane. The separated organic layer was washed in turn with hydrochloric acid (IN), aqueous sodium hydrogen carbonate, and brine, and dried over magnesium sulfate. Evaporation under reduced pressure gave a residue, which was triturated with diisopropyl ether to give N-(1-(4-fluorophenylsulfonyl)piperidin-4-yl)-N'-(4-fluorophenyl)urea (0.468 g).

NMR (DMSO-d 6 1.3-1.6 (2H, 1.75-1.95 (2H, m), 2.45-2.7 (2H, 3.35-3.6 (3H, 6.14 (1H, d, 7.03 (2H, t, J=9Hz), 7.34 (2H, dd, 9Hz), 7.50 (2H, t, J=9Hz), 7.75-7.95 (2H, 8.31 (1H, s) MASS (APCI)(m/z): 396 Example 17 To a suspension of N-(piperidin-4-yl)-4-fluorobenzamide (0.5 g) in dichloromethane (5 ml) were added pyridine (218 il), dichloromethane (5 ml) and benzoyl chloride (290 p1) at ambient temperature. After stirring for 3.5 hours, water ml) was poured into the mixture. An organic layer was separated, and washed with water and brine. After drying with magnesium sulfate, the solvents were removed under WO 00/42011 PCT/JPOO/0001 7 41 reduced pressure. A residue was purified by column chromatography (silica gel, toluene:ethyl acetate 1:1 to ethyl acetate) After rinse with diisopropyl ether, N-(1ben zoylpiperidin- 4-yl) -4 fluoroben zamide (515 rmg) was obtained.

NMR (DMSO-d 6 ):1.50 (2H, br s) 1.85 (2H, br s) 2.8-3.3 (28, in), 3.61 (18, in), 4.1 mn), 4.35 (1H, in), 7.29 (2H, t, J=8.9Hz), 7.3-7.5 (5H, in), 7.92 (2H, dci, 8.9Hz), 8.34 (1H, d, J=7.9Hz) MASS (APCI) 327 Exaple18 To a suspension of N-(piperidin--4-yl)-4-fluorobenzamide (556 mug) in dichloromethane (5 ml) were added pivaloyl chloride (0.37 ml), pyridine (0.24 ml) and N, N7dime thyl aminopyr idine (25 mug) at ambient temperature.

After stirring for 1 day, the mixture was diluted with dichloromethane, and washed with water and brine. After drying with magnesium sulfate, the solvents were removed under reduced pressure. After trituration with diisopropyl ether, N- (l-pivaloylpip eridin-4-yl) -4-fluorobenzamide (305 rug) was obtained.

NNR (DMSO-d 6 1.20 (9H, 1.41 (2H, in), 1.7-1.9 (2H, in), 2.91 (2H, br t, J=l1.9Hz), 4.07 (1H, in), 4.27 (2H, br d, J=13.3Hz), 7.29 (2H, t, J=8.9Hz), 7.92 (2H, dci, J=5.5, 8.9Hz), 8.30 (1H, d, J=7.8Hz) MASS (APCI) 329 To a suspension of N-(piperidin-4-yl)-4-fluorobenzamide (556 mg) in dichioromethane (6 ml) were added cyclopropanecarboxylic acid (0.20 ml), 1hydroxybenzotriazole (338 rug) and l-ethyl-3-(3dimethylaminopropyl)carboduimide hydrochloride '(480 rug) at ambient temperature. After stirring for 21 hours, the WO 00/42011 PCT/JP00O/00017 42 mixture was diluted with dichloromethane, and washed with water, saturated aqueous sodium hydrogen carbonate, and brine. After drying with magnesium sulfate, the solvents were removed under reduced pressure. After crystallization from diisopropyl ether, N-(l-cyclopropylcarbonylpiperidin-4yl)-4-fluorobenzamide (627 mg) was obtained.

NMR (DMSO-d 6 0.6-0.8 (4H, 1.2-1.6 (2H, m), 1.7-2.0 (2H, 1.85 (1H, 2.72 (1H, 3.21 (1H, 4.04 (1H, 4.30 (2H, 7.29 (2H, t, J=8.9Hz), 7.92 (2H, dd, J=5.6, 8.9Hz), 8.31 (1H, d, J=7.7Hz) MASS (APCI)(m/z): 313 Example l-tert-Butoxycarbonyl-4-(4-fluorophenylcarbamoyl)piperazine (0.30 g) was dissolved in a solution of hydrogen chloride in ethyl acetate (4N, 2 ml), and the solution was stirred at ambient temperature for 1 hour. The solvent was removed by evaporation under reduced pressure to give 1-(4-fluorophenylcarbamoyl)piperazine as a white powder, which was taken up into dichloromethane (3 ml), and to the mixture were added in turn pyridine (0.25 ml), 4-trifluoromethoxybenzoyl chloride (0.146 ml), and catalytic amount of N,N-dimethylaminopyridine. After stirring at ambient temperature for 12 hours, the mixture was washed in turn with hydrochloric acid aqueous sodium hydrogen carbonate, and brine, dried over magnesium sulfate, and evaporated under reduced pressure. The residue was chromatographed on silica gel (50 ml) eluting with 0%-3% methanol in dichloromethane to give 1-(4fluorophenylcarbamoyl)-4-(4-trifluoromethoxybenzoyl)piperazine (0.19 g).

NMR (DMSO-d 6 3.2-3.8 (8H, 7.08 (2H, t, J=9Hz), 7.35-7.5 (4H, 7.5-7.65 (2H, m) MASS (LD) 434.1 WO 00/42011 PCT/JP00/00017 43 Example 21 The following compound was obtained by using methyl chloroformate as a reactive derivative at the carboxy group according to a similar manner to that of Example l-Methoxycarbonyl-4-( 4 -fluorophenylcarbamoyl)piperazine NMR (DMSO-d 6 3.3-3.5 (8H, 3.62 (3H, 7.07 (2H, t, J=9Hz), 7.44 (2H, dd, J=5, 9Hz), 8.62 (1H, s) MASS (APCI)(m/z): 282 Example 22 A mixture of N-acetylpiperidine-4-carboxylic acid (514 mg), 1-hydroxybenzotriazole (405 mg), 1-ethyl-3-(3dimethylaminopropyl)carbodiimide hydrochloride (575 mg) and 4-fluoroaniline (284.2 ml) in dichloromethane (5 ml) was stirred for 18 hours at ambient temperature. The mixture was diluted with dichloromethane and washed with water, saturated aqueous sodium hydrogen carbonate, water, and brine. After drying with magnesium sulfate, the solvents were removed under reduced pressure. A residue was purified by column chromatography (silica gel 40 ml, dichloromethane:methanol 15:1). After trituration with diisopropyl ether, l-acetyl-4-(4-fluorophenyl)carbamoylpiperidine (532 mg) was obtained.

NMR (DMSO-d 6 1.3-1.7 (2H, 1.8 (2H, 2.01 (3H, 2.5 (2H, 3.05 (1H, br t, J=10.6Hz), 3.87 (1H, br d, J=14.1Hz), 4.40 (1H, br d, J=13.1Hz), 7.12 (2H, t, J=8.9Hz), 7.61 (2H, dd, J=5.1, 9.1Hz), 9.96 (1H, s) MASS (APCI)(m/z): 265 Example 23 A solution of l-acetylpiperazine-4-sulfonyl chloride (0.91 g) in chloroform (10 ml) were added 4-fluoroaniline WO 00/42011 PCT/JP00/00017 44 (0.38 ml) and triethylamine (0.56 ml) at ambient temperature.

After stirring for 6 days, the solvents were removed under reduced pressure. A residue was purified by column chromatography (silica gel 100 ml, dichloromethane:methanol 19:1). After rinse with diisopropyl ether, 1-acetyl-4-(4fluorophenyl)sulfamoylpiperazine (716 mg) was obtained.

NMR (CDC1 3 1.97 (3H, 3.09 (4H, 3.37 (4H, 7.20 (4H, 10.00 (1H, s) MASS (APCI)(m/z): 302 Example 24 To a solution of O-tert-butyl (l-acetylpiperidin-4yl)carbamate (0.97 g) in N,N-dimethylformamide (10 ml) was added 60% sodium hydride (0.18 g) at ambient temperature.

After stirring for 40 minutes, 4-fluorobenzyl bromide (0.6 ml) was added to the reaction mixture. After additional stirring for 4 hours, the reaction mixture was poured into a mixture of ethyl acetate (50 ml) and water (10 ml). An organic phase was separated and washed with water and brine.

After drying with magnesium sulfate, the solvents were removed under reduced pressure. A residue was purified by column chromatography (silica gel 100 ml, toluene:ethyl acetate 1:1 to After crystallization from diisopropyl ether and n-hexane, O-tert-butyl N-(4fluorobenzyl)-N-(l-acetylpiperidin-4-yl)carbamate (922 mg) was obtained.

NMR (DMSO-d 6 1.35 (9H, br 1.3-1.8 (4H, m), 1.95 (3H, 2.3-2.6 (1H, 2.97 (1H, 3.80 (1H, br d, J=15.2Hz), 4.0 (1H, 4.32 (2H, s), 4.2-4.6 (1H, 7.0-7.4 (4H, m), MASS (APCI) 295 Example To a solution of O-tert-butyl N-(4-fluorobenzyl)-N-(1- WO 00/42011 PCT/JP00/00017 acetylpiperidin-4-yl)carbamate (0.5 g) in dichloromethane ml) was added 4N hydrogen chloride in dioxane (5 ml). The reaction mixture was diluted with diisopropyl ether and the precipitates were collected by filtration. After drying under reduced pressure, l-acetyl-4-(4-fluorobenzyl)aminopiperidine hydrochloride (409 mg) was obtained.

NMR (DMSO-d 6

+D

2 0, 1.54 (2H, 2.02 (3H, s), 2.0-2.3 (2H, 2.4-2.7 (1H, 3.04 (1H, br t, J=12.1Hz), 3.29 (1H, 3.9 (1H, 4.17 (2H, s), 4.44 (1H, br d, J=13.6Hz), 7.27 (2H, t, J=8.9Hz), 7.66 (2H, br t, J=6.8Hz) MASS (APCI) 251 Example 26 To a solution of N-(l-acetylpiperidin-4-yl)-4fluorobenzamide (529 mg) in N,N-dimethylformamide (5 ml) was added sodium hydride (0.1 After stirring for 45 minutes, methyl iodide (623 ml) was added to the solution. After stirring for 45 minutes, the mixture was diluted with ethyl acetate (100 ml) and water (50 ml). An organic phase was separated, and washed with water and brine. After drying with magnesium sulfate, the solvents were removed under reduced pressure. After trituration with diisopropyl ether, N-(l-acetylpiperidin-4-yl)-N-methyl-4-fluorobenzamide (248 mg) was obtained.

NMR (DMSO-d 6 1.65 (4H, 2.00 (3H, 2.78 (3H, 3.8 (1H, 4.4 (1H, 2.0-4.6 (3H, br 7.26 (2H, t, J=8.9Hz), 7.46 (2H, dd, J=5.6, 8.7Hz) MASS (APCI)(m/z): 301 Example 27 A suspension of 1-acetylpiperazine (0.627 2-chloro- 4'-fluoroacetophenone (0.844 and potassium hydrogen carbonate (0.735 g) in acetonitrile (12 ml) was stirred at P:'OPERUgc8\14'-KI clmtns doc-(l3/l)11/ -46ambient temperature for 3 days. After removal of the solid by filtration, the filtrate was evaporated under reduced pressure to give a residue, which was chromatographed on silica gel (100 ml) eluting with methanol in dichloromethane. The objective compound of the free form was taken up into ethyl acetate (2 ml) and to the solution was added a solution of hydrogen chloride in ethyl acetate (4N, 2 ml). The resulting precipitate was collected by filtration, washed with diisopropyl ether, and dried in vacuo to give l-acetyl-4-(4-fluorophenylcarbonylmethyl)piperazine hydrochloride (1.47 g).

NMR (DMSO-d 6 2.06 (3H, 2.95-3.8 (6H, 3.9- 4.15 (1H, 4.2-4.45 (1H, 5.13 (2H, 7.48 (2H, t, J=9Hz), 8.09 (2H, dd, J=5, 9Hz) MASS (APCI) 265 Throughout this specification and the claims which follow, unless the context requires otherwise, the word "comprise", and variations such as "comprises" and "comprising", will be understood to imply the inclusion of a stated integer or step or group of integers or steps but not the exclusion of any other integer or step or group of integers or steps.

The reference to any prior art in this specification is not, and should not be taken as, an acknowledgment or any form or suggestion that that prior art forms part of the common general knowledge in Australia.

*^^STR s

Claims (9)

1. A compound of the general formula E R'-A-N X-Y-Q-R 2 3 R4 (I) wherein R 1 is Ci-C 6 alkanoyl, Cl-C6 alkoxycarbonyl, benzoyl, benzoyl substituted with halo C1-C6 alkoxy, C 1 -C 6 alkylsulfonyl, phenylsulfonyl, phenylsulfonyl substituted with halogen, or cyclo C 3 -C 6 alkylcarbonyl, R 2 is phenyl, phenyloxy or phenylamino, each phenyl of which may be substituted with halogen; pyridyl; or pyridylamino, A is a single bond, E is ethylene, X is CH, *oo R Y is -N-(wherein R 5 is hydrogen), 0 II Q is -C-or -SO 2 and R 3 and R 4 are taken together to form ethylene, :and pharmaceutically acceptable salt thereof. O A compound according to claim wherein Q is -C-

2. A compound according to claim 1, wherein Q is P:\OPER\Jgc\8))44M) ciimns.doc4)3AI13/)3 -48-

3. A compound according to claim 2, which is N-(l- acetylpiperidin-4-yl)-4-fluorobenzamide.

4. A process for preparing a compound of the general formula E R1-A-N X-Y-Q-R 2 R3 R4 (I) wherein R 1 R 2 R 3 R 4 A, E, Q, X and Y are each as defined in claim 1, which comprises: 1) reacting a compound of the formula: E R'-A-N CH-NH 2 13 1 4 R 3 R 4 (V) or its salt with a compound of the formula: HO-Qa-R 2 (III) or its reactive derivative at the carboxy or sulfo group, or 15 a salt thereof to provide a compound of the formula: E R -A-N CH-NH-Qa-R2 R 3 R 4 (Ic) 0 or its salt, in the above formulas, Qa is or -SO 2 and R 1 R 2 R 3 R 4 A and E are each as defined in claim 1; or 2) reacting a compound of the formula: S T P:\OPER\gc\l89)4-M4X clains.doc-3A13)3 -49- E R'-A-N CH-NH 2 R R 4 (V) or its salt with a compound of the formula: R 6 -NCO (IV) to provide a compound of the formula: 0 E 11 R'-A-N CH-NHCNH-R 6 R3 R 4 (Id) or its salt, in the above formulas, R 6 is phenyl which may be substituted with halogen; or pyridyl, and R 1 R 3 R 4 A and E are each as defined in claim 1; or 3) reacting a compound of the formula: E |t2 HN X-Y-Q-R R 3 R 4 (VI) or its salt with a compound of the formula: R1-A-OH (VII) or its reactive derivative at the carboxy or sulfo group, or a salt thereof to provide a compound of the formula: E R'-A-N X-Y-Q-R 2 R R (I) or its salt, in the above formulas, SSTqa R 1 R 2 R 3 R 4 A, E, X, Y and Q are each as defined in T claim 1. P:\OPERUgc\l 9')04-I4 cllims.doc-043/0l3/ A pharmaceutical composition comprising a compound of claim 1, as an active ingredient, in association with a pharmaceutically acceptable, substantially non-toxic carrier or excipient.

6. A compound of claim 1 for use as a medicament.

7. A method for therapeutic treatment and/or prevention of amnesia, dementia or schizophrenia which comprises administering an effective amount of a compound of claim 1 to mammals.

8. Use of a compound of claim 1 for manufacture of a medicament for treating and/or preventing amnesia, dementia or schizophrenia in mammals.

9. A compound of claim 1 substantially as hereinbefore described with reference to the Examples.

10. A process according to claim 4 substantially as hereinbefore described with reference to the Examples. DATED this 3 rd day of March, 2003 Fujisawa Pharmaceutical Co., Ltd. by DAVIES COLLISON CAVE Patent Attorneys for the Applicant *o ro o