AU7494900A - Signal counting for in situ hybridization - Google Patents

Signal counting for in situ hybridization

Info

Publication number
AU7494900A
AU7494900A AU74949/00A AU7494900A AU7494900A AU 7494900 A AU7494900 A AU 7494900A AU 74949/00 A AU74949/00 A AU 74949/00A AU 7494900 A AU7494900 A AU 7494900A AU 7494900 A AU7494900 A AU 7494900A
Authority
AU
Australia
Prior art keywords
situ hybridization
signal counting
counting
signal
hybridization
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU74949/00A
Inventor
Lukas Budendorf
Edward R. Dougherty
Artyom M. Grigoryan
Olli P. Kallioniemi
Juha Kononen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
National Institutes of Health NIH
Original Assignee
US Department of Health and Human Services
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by US Department of Health and Human Services filed Critical US Department of Health and Human Services
Publication of AU7494900A publication Critical patent/AU7494900A/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429—Signal processing
    • G01N15/1433—Signal processing using image recognition
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00—Scenes; Scene-specific elements
    • G06V20/60—Type of objects
    • G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
    • G06V20/695—Preprocessing, e.g. image segmentation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Physics & Mathematics (AREA)
  • Signal Processing (AREA)
  • Multimedia (AREA)
  • Theoretical Computer Science (AREA)
  • Dispersion Chemistry (AREA)
  • Pathology (AREA)
  • Biomedical Technology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Image Processing (AREA)
AU74949/00A 1999-09-17 2000-09-15 Signal counting for in situ hybridization Abandoned AU7494900A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US15460199P 1999-09-17 1999-09-17
US60/154601 1999-09-17
PCT/US2000/025465 WO2001020044A2 (en) 1999-09-17 2000-09-15 Signal counting for in situ hybridization

Publications (1)

Publication Number Publication Date
AU7494900A true AU7494900A (en) 2001-04-17

Family

ID=22551987

Family Applications (1)

Application Number Title Priority Date Filing Date
AU74949/00A Abandoned AU7494900A (en) 1999-09-17 2000-09-15 Signal counting for in situ hybridization

Country Status (2)

Country Link
AU (1) AU7494900A (en)
WO (1) WO2001020044A2 (en)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7219016B2 (en) 2001-04-20 2007-05-15 Yale University Systems and methods for automated analysis of cells and tissues
FR2826977B1 (en) * 2001-07-03 2004-07-16 Imstar S A METHOD AND SYSTEM FOR DETECTING INTER-CHROMOSOMAL IMBALANCE BY IN SITU HYBRIDIZATION OF FLUORESCENT PROBES (FISH) ON INTERPHASE CELL CORES
US7408176B2 (en) 2003-06-19 2008-08-05 Applied Precision, Llc System and method employing photokinetic techniques in cell biology imaging applications
US8068988B2 (en) 2003-09-08 2011-11-29 Ventana Medical Systems, Inc. Method for automated processing of digital images of tissue micro-arrays (TMA)
WO2005027015A2 (en) 2003-09-10 2005-03-24 Bioimagene, Inc. Method and system for quantitatively analyzing biological samples
WO2005076216A2 (en) * 2004-02-03 2005-08-18 Bioimagene, Inc. Method and system for automaticed digital image based flourescent in situ hybridization (fish) analysis
AU2007248424B2 (en) 2006-05-05 2013-04-18 Historx, Inc. Immunohistochemical methods for determining signal transduction activity in tumors
EP2047262A4 (en) 2006-07-13 2011-03-16 Univ Yale Methods for making cancer prognoses based on the subcellular localization of biomarkers
GB0807466D0 (en) * 2008-04-24 2008-05-28 Univ Nottingham Trent Improvements relating to assays including a labelled reagent
JP5916991B2 (en) * 2010-11-09 2016-05-11 オリンパス株式会社 Methods for analyzing genetically abnormal cells
US8755580B2 (en) 2012-03-17 2014-06-17 Sony Corporation Flourescent dot counting in digital pathology images
US9378407B2 (en) 2012-09-11 2016-06-28 Neogenomics Laboratories, Inc. Automated fish reader using learning machines
US9042631B2 (en) 2013-01-24 2015-05-26 General Electric Company Method and systems for cell-level fish dot counting
US9488639B2 (en) * 2013-02-25 2016-11-08 Flagship Biosciences, Inc. Cell-based tissue analysis
CN112424825A (en) * 2018-05-15 2021-02-26 文塔纳医疗***公司 Quantification of signal in stained aggregates
CN111175267A (en) * 2020-01-18 2020-05-19 珠海圣美生物诊断技术有限公司 Cell interpretation method and system based on FISH technology
CN114463609B (en) * 2022-04-13 2022-07-05 珠海横琴圣澳云智科技有限公司 Method and device for determining fluorescent mixed points in cell microscopic image
CN114739970B (en) * 2022-06-09 2022-09-16 珠海横琴圣澳云智科技有限公司 Method and device for judging fluorescent signal point fracture
CN116144739A (en) * 2023-03-06 2023-05-23 中国医学科学院北京协和医院 Gene rearrangement signal recording method based on fluorescence in situ hybridization of separation probe

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5817462A (en) * 1995-02-21 1998-10-06 Applied Spectral Imaging Method for simultaneous detection of multiple fluorophores for in situ hybridization and multicolor chromosome painting and banding
PT1068528E (en) * 1998-02-25 2006-12-29 Us Gov Health & Human Serv Method and apparatus for making an array for rapid molecular profiling

Also Published As

Publication number Publication date
WO2001020044A2 (en) 2001-03-22
WO2001020044A3 (en) 2002-05-10

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Legal Events

Date Code Title Description
MK6 Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase