AU723180B2 - Novel compound with platelet aggregation inhibitor activity - Google Patents

Novel compound with platelet aggregation inhibitor activity Download PDF

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AU723180B2
AU723180B2 AU52084/98A AU5208498A AU723180B2 AU 723180 B2 AU723180 B2 AU 723180B2 AU 52084/98 A AU52084/98 A AU 52084/98A AU 5208498 A AU5208498 A AU 5208498A AU 723180 B2 AU723180 B2 AU 723180B2
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AU5208498A (en
Inventor
Kenji Asai
Emiko Hatsushiba
Hiroyuki Iida
Midori Ishikawa
Yasuko Isomura
Kiyoaki Katano
Mami Kawaguchi
Tomoaki Miura
Shokichi Ohuchi
Masaro Shimizu
Eiki Shitara
Takashi Tsuruoka
Kazue Yaegashi
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Meiji Seika Kaisha Ltd
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Meiji Seika Kaisha Ltd
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D413/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms
    • C07D413/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms containing two hetero rings
    • C07D413/06—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms containing two hetero rings linked by a carbon chain containing only aliphatic carbon atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C237/00—Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by amino groups
    • C07C237/24—Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by amino groups having the carbon atom of at least one of the carboxamide groups bound to a carbon atom of a ring other than a six-membered aromatic ring of the carbon skeleton
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C257/00—Compounds containing carboxyl groups, the doubly-bound oxygen atom of a carboxyl group being replaced by a doubly-bound nitrogen atom, this nitrogen atom not being further bound to an oxygen atom, e.g. imino-ethers, amidines
    • C07C257/10—Compounds containing carboxyl groups, the doubly-bound oxygen atom of a carboxyl group being replaced by a doubly-bound nitrogen atom, this nitrogen atom not being further bound to an oxygen atom, e.g. imino-ethers, amidines with replacement of the other oxygen atom of the carboxyl group by nitrogen atoms, e.g. amidines
    • C07C257/16—Compounds containing carboxyl groups, the doubly-bound oxygen atom of a carboxyl group being replaced by a doubly-bound nitrogen atom, this nitrogen atom not being further bound to an oxygen atom, e.g. imino-ethers, amidines with replacement of the other oxygen atom of the carboxyl group by nitrogen atoms, e.g. amidines having carbon atoms of amidino groups bound to carbon atoms of rings other than six-membered aromatic rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C257/00—Compounds containing carboxyl groups, the doubly-bound oxygen atom of a carboxyl group being replaced by a doubly-bound nitrogen atom, this nitrogen atom not being further bound to an oxygen atom, e.g. imino-ethers, amidines
    • C07C257/10—Compounds containing carboxyl groups, the doubly-bound oxygen atom of a carboxyl group being replaced by a doubly-bound nitrogen atom, this nitrogen atom not being further bound to an oxygen atom, e.g. imino-ethers, amidines with replacement of the other oxygen atom of the carboxyl group by nitrogen atoms, e.g. amidines
    • C07C257/18—Compounds containing carboxyl groups, the doubly-bound oxygen atom of a carboxyl group being replaced by a doubly-bound nitrogen atom, this nitrogen atom not being further bound to an oxygen atom, e.g. imino-ethers, amidines with replacement of the other oxygen atom of the carboxyl group by nitrogen atoms, e.g. amidines having carbon atoms of amidino groups bound to carbon atoms of six-membered aromatic rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C279/00—Derivatives of guanidine, i.e. compounds containing the group, the singly-bound nitrogen atoms not being part of nitro or nitroso groups
    • C07C279/04—Derivatives of guanidine, i.e. compounds containing the group, the singly-bound nitrogen atoms not being part of nitro or nitroso groups having nitrogen atoms of guanidine groups bound to acyclic carbon atoms of a carbon skeleton
    • C07C279/14—Derivatives of guanidine, i.e. compounds containing the group, the singly-bound nitrogen atoms not being part of nitro or nitroso groups having nitrogen atoms of guanidine groups bound to acyclic carbon atoms of a carbon skeleton being further substituted by carboxyl groups
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C279/00—Derivatives of guanidine, i.e. compounds containing the group, the singly-bound nitrogen atoms not being part of nitro or nitroso groups
    • C07C279/16—Derivatives of guanidine, i.e. compounds containing the group, the singly-bound nitrogen atoms not being part of nitro or nitroso groups having nitrogen atoms of guanidine groups bound to carbon atoms of rings other than six-membered aromatic rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D211/00—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings
    • C07D211/04—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom
    • C07D211/06—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members
    • C07D211/08—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members with hydrocarbon or substituted hydrocarbon radicals directly attached to ring carbon atoms
    • C07D211/18—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members with hydrocarbon or substituted hydrocarbon radicals directly attached to ring carbon atoms with substituted hydrocarbon radicals attached to ring carbon atoms
    • C07D211/34—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members with hydrocarbon or substituted hydrocarbon radicals directly attached to ring carbon atoms with substituted hydrocarbon radicals attached to ring carbon atoms with hydrocarbon radicals, substituted by carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D211/00—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings
    • C07D211/04—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom
    • C07D211/06—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members
    • C07D211/36—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C07D211/60—Carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals
    • C07D211/62—Carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals attached in position 4
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D263/00—Heterocyclic compounds containing 1,3-oxazole or hydrogenated 1,3-oxazole rings
    • C07D263/02—Heterocyclic compounds containing 1,3-oxazole or hydrogenated 1,3-oxazole rings not condensed with other rings
    • C07D263/08—Heterocyclic compounds containing 1,3-oxazole or hydrogenated 1,3-oxazole rings not condensed with other rings having one double bond between ring members or between a ring member and a non-ring member
    • C07D263/10—Heterocyclic compounds containing 1,3-oxazole or hydrogenated 1,3-oxazole rings not condensed with other rings having one double bond between ring members or between a ring member and a non-ring member with only hydrogen atoms, hydrocarbon or substituted hydrocarbon radicals, directly attached to ring carbon atoms
    • C07D263/14—Heterocyclic compounds containing 1,3-oxazole or hydrogenated 1,3-oxazole rings not condensed with other rings having one double bond between ring members or between a ring member and a non-ring member with only hydrogen atoms, hydrocarbon or substituted hydrocarbon radicals, directly attached to ring carbon atoms with radicals substituted by oxygen atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D495/00—Heterocyclic compounds containing in the condensed system at least one hetero ring having sulfur atoms as the only ring hetero atoms
    • C07D495/02—Heterocyclic compounds containing in the condensed system at least one hetero ring having sulfur atoms as the only ring hetero atoms in which the condensed system contains two hetero rings
    • C07D495/04—Ortho-condensed systems
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D513/00—Heterocyclic compounds containing in the condensed system at least one hetero ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for in groups C07D463/00, C07D477/00 or C07D499/00 - C07D507/00
    • C07D513/02—Heterocyclic compounds containing in the condensed system at least one hetero ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for in groups C07D463/00, C07D477/00 or C07D499/00 - C07D507/00 in which the condensed system contains two hetero rings
    • C07D513/04—Ortho-condensed systems
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C2601/00—Systems containing only non-condensed rings
    • C07C2601/12—Systems containing only non-condensed rings with a six-membered ring
    • C07C2601/14—The ring being saturated

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  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Description

AUSTRALIA
PATENTS ACT 1990 DIVISIONAL APPLICATION NAME OF APPLICANT(S): 0*
S
S
S.
S*
S S S S
S
S
*SSS
S
S Meiji Seika Kabushiki Kaisha ADDRESS FOR SERVICE: DAVIES COLLISON CAVE Patent Attorneys 1 Little Collins Street Melbourne, 3000.
INVENTION TITLE: Novel compound with platelet aggregation inhibitor activity The following statement is a full description of this invention, including the best method of performing it known to us: Q:AOPERRMHTB26381-94.DIV 1411198 FIELD OF THE INVENTION The present invention relates to a cyclohexene and a nitrogen-containing heterocyclic derivative for inhibiting the aggregation of platelets, and a pharmaceutical composition for the treatment and prophylaxis of thrombotic diseases comprising as effective ingredient at least one of these derivatives.
BACKGROUND TECHNOLOGIES Caldiovascular diseases are- increased along with the change of dietary habits and the increase of advanced ages.
Almost fifty percent of these diseases may be caused by thrombus.
Platelets in plasma are mainly associated with the Sformation of thrombus in organisms. For the purpose of the treatment and prophylaxis of thrombotic diseases in clinical practice, there have been used a medicine which suppresses the functions of platelet or inhibits the aggregation of platelets, for example, aspirin which inhibits cyclooxygenase and ticlopidine which activates adenylcyclase.
In recent years, glycoproteins on platelet membrane have been progressively analyzed. As the results, it has been elucidated that the glycoprotein called GPIIb/IIIa is a receptor of fibrinogen. This has therefore led to the expectation that a GPIIb/IIIa antagonists would become an inhibitor of platelet aggregation having a novel action mechanism effectively used for the treatment and prophylaxis of the thrombotic diseases (Trends in Pharmacological Science, 13, 413, 1992). The compounds as the GPIIb/IIIa antagonist include a monoclonal antibody (Ann. New York Acad. Sci., 614, 193, 1991), a tripeptide derivative comprising arginine-glycine-aspartic acid Med. Chem., 2040, 1992), amidinophenyl derivative Med. Chem., 35, 4393, 1992; Japanese Patent Laid-Open Publication Nos. 264068/1992 and 334351/1992), and a tyrosine derivative Med. Chem., 35, 4640, 1992).
P:\OPER\RMH\62638-94.SPE 14/1/98 -2- It is also desired to be developed a medicine having no side effects such as hemorrhage and a highly selective function as a therapeutic or prophylactic agent of thrombotic diseases.
SUMMARY OF THE INVENTION The present inventors have now found that a certain kind of compound acts as a GPIIb/IIIa antagonist.
Thus, a preferred object of the present invention is to provide a novel compound which inhibits the aggregation of platelets.
Another preferred object of the present invention is to provide a pharmaceutical composition comprising a novel compound having the aforementioned effect.
A further preferred object of the present invention is to provide a method for the treatment or prophylaxis of thrombotic 15 diseases which comprises administering a novel compound having the above activity.
Yet'another preferred object of the present invention is to provide the use of the novel compound having the above activity for preparing a pharmaceutical composition used for the therapy or prophylaxis of thrombotic disorders.
The compound according to the present invention is represented by the formula A-B- R or a pharmaceutically acceptable salt and solvate thereof, wherein
R
1 represents a group -W-(CH 2 )i-COOR 3 where W represents -0or a bond, R 3 represents hydrogen, lower alkyl, C 5 7 cycloalkyl or an ester moiety which may be removed under a physiological condition, and i is an integer from 1 to 4.
R
2 represents hydrogen or a group -W-(CH 2 )i-COOR 3 where W,
R
3 and i have the same meanings as defined above, or a group -OR 4 where R 4 represents hydrogen, lower alkyl, mono-lower alkylaminocarbonyl or phenyl-lower alkyl, X represents CH or N, Y represents a group -(CO)k-N(R 5 where k is 0 or i,
R
5 represents hydrogen; lower alkyl in which one or more hydrogen atoms may be substituted by hydroxyl, halogen, amino, carboxyl, lower alkoxy, lower alkylamino, or lower alkoxycarbonyl; phenyl-lower alkyl in which one or more hydrogen atoms in the phenyl moiety may be substituted by hydroxyl, halogen, amino, carboxyl, lower alkoxy, lower alkylamino, lower alkoxycarbonyl or halo-lower alkyl; or acyl, Z represents a bond, or a group -(CH2)m-CO- or a group
-(CH
2 )m-CHR 6 where m is an integer from 1 to 3 and R represents hydrogen or hydroxyl, (ii) a group -CO-(CH2)m-N(RS)-(CO)k-, where k, m and R 5 have the same meanings as defined above, or (iii) a group -(CO)k-Het, where represents a five- or sixmembered heterocyclic moiety which contains 1 to 4 nitrogen atoms, which may also optionally contain an oxygen atom or a sulfur atom when the heterocyclic moiety contains 1 or 2 nitrogen atoms, and k is 0 or 1, A represents the following group (II):
R
8 7 wherein D represents a group where s is an integer from 1 to 4, or a group and
R
7 and RS represent independently hydrogen, lower alkyl, acyl, aromatic acyl which may be substituted, or amidino, P:\OPER\PDB\52084-98.spec.doc19/06/00 -4- B represents a bond, C 1 -6 alkylene or C2-6 alkenylene.
DETAILED DESCRIPTION OF THE INVENTION Compound of the Formula (I) The term "lower alkyl" as a group or a portion of a group herein means a straight or branched alkyl chain having 1 to 6, preferably 1 to 4 carbon atoms. The terms "alkylene" and "alkenylene", respectively, mean a bivalent group derived by removing hydrogen atoms from both terminals of a straight or branched alkane or alkene chain. The term halogen atom means fluorine, chlorine, bromine or iodine. Furthermore, the term "haloalkyl" means an alkyl group in which one or more hydrogen atoms are substituted by halogen atoms.
In the group -W-(CH 2 )i-COOR 3 as R 1 i represents preferably an integer of from 1 or 2. R 3 represents preferably a hydrogen atom or lower alkyl which is preferably C1_ 6 alkyl, more preferably C 1 4 alkyl, and specifically includes methyl, ethyl, n-propyl, iso-propyl, or iso-, sec- or t-butyl.
Specific examples of an ester moiety as R 3 which may be removed under physiological conditions include pivaloyloxymethyl, 1- (cyclohexyloxy-carbonyloxy)ethyl or (5-methyl-2-oxo-1,3-dioxol-4yl)methyl.
Preferred examples of R include hydrogen and the group 3 W-(CH2)i-COOR 3 Preferred examples of the group -W-(CH2)i-COOR as.
icu 1 1 2
R
2 include those which are the same as in R When R and R .represent the group -W-(CH 2 )i-COOR3, they may be the same or different.
In the group -OR 4 as R 2 lower alkyl as R 4 represents preferably C1-6 alkyl, more preferably C1-4 alkyl, and specifically include methyl, ethyl, n-propyl, iso-propyl, or n-, iso-, sec- or t-butyl. The mono-lower alkylaminocarbonyl group as R 4 represents preferably mono-C 1 6 alkylaminocarbonyl, more preferably mono-C 1 4 alkylaminocarbonyl. Preferred examples of Sphenyl-lower alkyl as R 4 include phenyl C 1 4 alkyl such as benzyl.
The substituted positions of R 1 and R" is not limited in particular, but it is preferably in the meta- and/or parapositions to the position that the group Y is attached to.
In the group -(CO)k-N(R 5 as Y, R 5 represents hydrogen, lower alkyl, phenyl-lower alkyl or acyl, preferably hydrogen, C 1 6 alkyl (more preferably C1-4 alkyl, more specifically methyl, ethyl, n-propyl, iso-propyl, or iso, sec- or t-butyl), benzyl, 2-phenylethyl, 3-phenylpropyl or lower alkylcarbonyl (preferably C1-6 alkylcarbonyl, more preferably C1-4 alkylcarbonyl) group, and aromatic acyl such as benzoyl, anaphthoyl and p-naphthoyl.
One or more hydrogen atoms in the lower alkyl may be substituted, and specific examples of the substituents include hydroxyl; halogen such as preferably chlorine, bromine and fluorine; amino; carboxyl; lower alkoxy such as preferably methoxy, ethoxy, n-propoxy and iso-propoxy; lower alkylamino such as preferably methylamino, ethylamino, propylamino, dimethylamino and diethylamino; and lower alkoxycarbonyl such as preferably methoxycarbonyl, ethoxycarbonyl, n-propoxycarbonyl and iso-propoxycarbonyl.
Furthermore, one or more hydrogen atoms of the phenyl moiety in the phenyl-lower alkyl group may be substituted.
Specific examples of the substituent include hydroxyl; halogen; amino; carboxyl; lower alkoxy such as preferably methoxy, ethoxy and propoxy; lower-alkylamino such as preferably methylamino, 15 ethylamino, propylamino, dimethylamino and diethylamino; lower alkoxycarbonyl such as preferably methoxycarbonyl, ethoxycarbonyl S"and propoxycarbonyl; and halo-lower alkyl such as preferably trifluoromethyl and trifluoroethyl.
In the group -(CO)k-N(R 5 k is 0 or 1; means 20 a linkage when k is 0. In the group -(CH2)m-CO- or -(CH2) -CHR as Z, m preferably is 1 or 2. When k is 0, Z preferably represents the group -(CH )m-CHR6, wherein R 6 represents hydrogen.
In the group -CO-(CH )-N(R5 as Y, preferred examples of R 5 include those described above.
In the group -(CO)k-Het- as Y, Het represents a five- or six-membered heterocyclic moiety containing 1 to 4, preferably 1 or 2 nitrogen atoms. The heterocyclic ring may optionally contain further one oxygen or sulfur atom when the ring contains 1 or 2 nitrogen atoms. Preferred examples of the heterocyclic moiety include heterocyclic moieties formed by leaving two hydrogen atoms from pyrrole, imidazole, pyrazole, 1,2,3-triazole, 1,2,4-triazole, tetrazole, oxazole, isoxazole, thiazole, isothiazole, 1,2,4-oxadiazole, 1,3,4-oxadiazole, 1,2,3thiadiazole, 1,3,4-thiadiazole, 1,2,4-thiadiazole, pyridine, pyridazine, pyrazine, oxazine or thiazine. According to the preferred embodiment of the present invention, preferred heterocyclic moieties include oxazole, isoxazole, thiazole and isothiazole moieties.
The position of the group Y to a benzene ring or a pyridine ring and to the group B is not limited particularly.
Moreover, the bonding may be either a carbon-carbon bond or a carbon-nitrogen bond. According to the preferred embodiment of the present invention, the group Y is introduced into an ortho position to X.
Moreover, in the group (II) as A, preferred examples of the lower alkyl as R and R include methyl, ethyl, propyl, isopropyl, and sec- and t-butyl. Preferred examples of the lower acyl as R 7 and R 8 include formyl and lower alkylcarbonyl such as acetyl, propionyl and butyryl. Preferred examples of the .7 8 aromatic acyl as R and R include benzoyl, a-naphthoyl and 3naphthoyl. One or more hydrogen atoms in the aromatic acyl group may be substituted, and preferred examples of the substituent include amidino, amino, chlorine, fluorine or hydroxyl, particularly benzoyl group substituted by one of the above 20 substituents at either one of the m- or p-positions.
In -(CH 2 as D in the group s preferably represents an integer of 1 to 3, more preferably 1 or 2.
,In the group the groups D and B may be bonded to the cyclohexane ring in either configurations of cis- or trans-types 25 depending on the formations of the bonds. Both of the isomers are included in the present invention.. The trans-isomer is particularly preferred.
In the groups (III) and (IV) as A, R 9 represents hydrogen, lower alkyl or amidino group. One or more hydrogen atoms in the lower alkyl group may be substituted, and specific examples of the substituent include hydroxyl; halogen such as preferably chlorine, bromine or fluorine; amino; and lower alkylamino such as preferably methylamino, ethylamino, dimethylamino and diethylamino.
The bonding position of the group (III) and the group B is not limited particularly, but it is preferably at the 4position to R 9
N.
In the group p and q independently represent an integer of 1 to 3, and p q is in the range of 3 to preferably 3 or 4.
In the preferred example of the group p is 2, q is 2 or 3, either E or H represents -NR 10 or and the other represents a bond, and both of F and G represent -CR 0 or one of F or G represents -CR 10 and the other represents or Specific examples preferably include 4,5,6,7tetrahydrothieno[3,2-c]pyridin- 2 -yl or -3-yl, 4,5,6,7tetrahydrothieno[2,3-c]pyridin- 2 -yl or -3-yl, l-methyl-4,5,6,7tetrahydro-pyrrolo[3,2-c]pyridin-2-yl.or -3-yl, l-methyl-4,5,6,7tetrahydropyrrolo[2,3-c]pyridin-2-yl or -3-yl, 4,5,6,7tetrahydrofuro[3,2-c]pyridin- 2 -yl or -3-yl, 4,5,6,7- 15 tetrahydrofuro[2,3-c]pyridin- 2 -yl or -3-yl, 5,6,7,8-tetrahydro- 4H-thieno[3,2-c]azepin-2-yl or -3-yl, or 5,6,7,8-tetrahydro-4Hthieno[2,3-d]azepin-2-yl or -3-yl.
In the group R 10 and R 1 1 preferably represent hydrogen, C1-4 alkyl or phenyl-C_ 4 alkyl such as benzyl.
20 The C 1 6 alkylene as B is preferably C 1 4 alkylene, more preferably C 1 3 alkylene. The C2- 6 alkylene as B is more •preferably C2-4 alkylene.
The preferred group of the compounds of the present invention includes a group wherein Y represents a group -(CO)k-
N(R
5 where k is 1, or a group -(CO)k-Het-, and A represents the group According to the more preferred embodiment, in the group, B represents a bond and X represents CH. According to the further preferred embodiment, in the group, Z represents a group m-CO- or a group -(CHI)m-CHR 6 where R6 represents hydrogen.
According to another preferred embodiment of the present invention, there provides a group in which Y represents a group -(CO)k-N(R 5 where k is 1, or a group -(CO)k-Het-,
A
represents the group (III), and B represents a bond or C1_ 6 alkylene. In this group, a compound wherein Z represents a group -CO- or a group m-CHR 6 where R 6 represents hydrogen and B represents a bond or C1-6 alkylene is more preferred.
According to further preferred embodiment of the present invention, there provides a group wherein Y represents a group -(CO)k-N(R 5 where k is 1, or a group -NHCO- or a group (CO)k-Het-, A represents the group and B represents a bond or C1-6 alkylene. In this group, the compound in which A represents the group (IV) where either E or H represents -NR 1 or the other represents a bond, and F or G represents CR0=, is preferred. Furthermore, in the group, the compound in which either p or q is 1 and the other is 2 or both of p and q is 2, is preferred. Moreover, in the group, the compound in which .either F or G represents -CR 10 where R 1 0 represents hydrogen, the other represents -CR where Ri is not hydrogen, is more preferred. In the group, the compound in which A represents the group where either E or H represents -NR or the other represents a bond, and either F or G represents -CR and the other represents -NR 10 or is another preferred one. Moreover, the compound in which Y represents the group -(CO)k-Het-, is another preferred one.
The compound according to the present invention can be in the form of a salt. Such a salt includes a pharmacologically acceptable non-toxic salt. Preferred examples of the salt include inorganic salts such as a sodium salt, a potassium salt, a magnesium salt and a calcium salt, acid addition salts such as a trifluoroacetate salt, a hydrochloride salt, an oxalate salt and a methanesulfonate salt a citrate salt, and amino acid salts such as a glutamate salt and an aspartate salt.
The compound according to the present invention can be in the form of a solvate. The solvate preferably includes a hydrate and an ethanolate.
Preparation of the Compound represented by the Formula (I) The compound according to the present invention can be prepared by the following process: In the case of the compound in which Y represents the group -(CO)k-N(R 3 where Z represents a bond, a group(CH) m-CO- or a group )m-CHR 6 where R represents hydrogen, but R 5 does not represent lower acyl: This compound can be prepared by reacting the compound represented by the formula A-B-(CO)k-L
(V)
wherein A, B and k have the same meanings as defined in the formula and L represents halogen, alkylsulfonyloxy or arylsulfonyloxy, with the compound represented by the formula
(VI):
H-N-Z
R
I (VI) wherein R1 R 2
R
5 and X have the same meanings as defined in the 1 2 formula provided that when R 1 and R 2 contain a carboxyl group or a hydroxyl group, the carboxyl group and the hydroxyl group may be protected, Z represents a bond, a group(CH2)M-COor a group -(CH-)m-CHR 6 where R 6 represents a hydrogen atom, in the presence or absence of a base in an inert solvent at a temperature of -30 to 100:C, preferably -20 to 80 C for 30 minutes to 48 hours, preferably 1 to 10 hours, and removing the protective groups, if necessary.
The compound represented by the formula can be prepared according to the known method, for example, described in Chem. Pharm. Bull., 34 3747 (1986), Ark. Kemi., 32, 217 (1970), and Japanese Patent Laid-Open Publication Nos.
150687/1982 and 2992/1988.
The compound represented by the formula (VI) wherein X represents -CH= can be also prepared according to the method described in Japanese Patent Laid-Open Publication Nos.
65240/1977 and 158922/1986.
Moreover, the compound represented by the formula (VI) wherein X represents N and represents a group -(CHI)m-CO- can be prepared by the process according to the following scheme:
RI
HOH 2 C R2
N
Oxidation
OHR
0 H
RN
C H 3
H
2 M-FM gB r
HC
or CH 3
-(CFH{
2 F L i -C H 3
H
2 Oxidation
R
CHj-(C H ~COR 3 2 -N R t
N
15 S. S *9 *9 9 9
S.
20 Halogenation H a I- (CH) -CO0 a. 1)IN aN 3 2) Reduction or b. NH 2
RD
*9 '4 5.
5* 5 9 4S*90* 25H-N- (OH2) 01-C 0-R~ 1 225 wherein R R and n have the same rneanings as def ined in the formula provided that when Riand R2 contain a carboxyl group and a hydroxyl group, the carboxyl group and the hydroxyl group may be protected, and Hal represents a halogen atom.
The compound represented by the formula (VI) wherein X represents N and Z represents a group (CH-,))MCHR where R 6 represents hydrogen, can be prepared by halogenating followed by reducing the hydroxyl group of the compound represented by the formula wherein Z in Y represents -(CHj)M- CHOH-. The r i, halogenation can be carried out by using a reagent such as thionyl chloride or phosphorus pentachloride in an inert solvent such as chloroform or dichloromethane at a temperature of to 100'C, preferably -10'C to 30'C. Reduction is carried out with a trialkyltin hydride such as tributyltin hydride or by catalytic reduction with use of a catalyst such as palladium on carbon or platinum oxide in an inert solvent as benzene, toluene or methanol at a temperature of O'C to 100:C, preferably 10:C to In the case of the compound in which Y represents the group wherein Z represents a group -(CH) m
CHR
6 where R represents OH, but R 5 does not represent lower acyl: This compound can be prepared by reducing the ketone group of the compound represented 15 by the formula wherein Y represents a group R)- 10 1 2 S: (CH2) wherein amino in R 0 and, when R and R 2 contain a carboxyl group and a hydroxyl group, the carboxyl and hydroxyl group may be protected, with an appropriate reducing agent such as sodium borohydride, and removing the protective groups, if 20 necessary, or reacting the compound represented by the formula (V) with the compound represented by the following formula (VII): .t.
25 H-N- (CHZ -CH R a R
R
wherein R R 5 X and n have the same meanings as defined in the formula and R6 represents hydroxyl, under substantially the same conditions as in the above process The compound of the formula (VII) can be prepared, for example, by reducing a compound represented by the formula:
R
H-N- (CH -CO R2 Rs N with a suitable reducing agent catalytic reduction).
In the case of the compound wherein Y represents the group wherein R 5 represents lower acyl: This compound can be prepared by reacting a compound represented by the formula A-B-CHO with the compound represented by the above formula (VI) wherein R 5 represents hydrogen or the compound represented by the formula (VII) wherein R 5 represents hydrogen in the presence of a reducing agent such as NaCNBH 3 or H2/Pd-C to give the compound represented by the formula (I) wherein R 5 represents hydrogen, which is then reacted with an appropriate acylating agent such as acetic anhydride, acetyl chloride or benzoyl chloride.
In the case of the compound wherein Y represents the group -CO-(CH) ,-N(R 15 Th_ compound can be prepared by reacting the compound 9. represented by the formula: "4 A-B-CO-(CH) -N(R)H (VIII) wherein R5 and m have the same meanings as defined in the formula with the compound represented by the formula (IX):
RI
L- (CO) -R 2
X
wherein R, R 2 X and k have the same meanings as defined in the formula and L represents halogen, alkyl or allylsulfonyloxy, in the presence or absence of a base such as triethylamine, Nmethylmorpholine, pyridine, N,N-dimethyl-aminopyridine in an inert solvent at -30'C to 100:C, preferably -10'C to 50:C for minutes to 48 hours, preferably 1 to 24 hours, and removing the protective group, if necessary.
The compound represented by the formula (VIII) can be prepared according to the following scheme: CH 3 -(CH 2 m- 1 -MB r or CH 3 (CH 9 )nFL
A-B-CHO
A-B-CHOH- (CH _-CH 2 t A-B-CO- (CH2) -CH 3 A-B-CO- (CH2)m-Ha i Oxidation 3 t) F 3 CSO 2 S i (CH 3 3 2) NBS or NCS a. i)NaN 3 2) Reduction b. H NR 1
S
4 a.
3 .A-B-CO- (CH2) -N (R 0.
In the case of the compound wherein Y represents the group -(CO)k-Het-, wherein k denotes 0: This compound can be prepared by intramolecular condensation of the Y in the compound represented by the general formula wherein Y represents the group -(CO)k-N(R 5 )m- CO- to form a cyclic compound. Specifically, it can be prepared by reacting the corresponding compound represented by the formula in the presence of an acid at a temperature of 0 to 100:C for 1 minute to 6 hours. The suitable acid includes inorganic acids such as sulfuric acid, hydrochloric acid and hydrobromic acid, particularly sulfuric acid.
In the case of the compound wherein Y represents (CO)k-Het-, wherein k 1: This compound can be prepared according to the following scheme: Ri A-B-CHO Het -R
IX
(XI)
RI
A-B-CH-Het
R
2 I
X
OH
R
A-B-CO-Het R2 wherein R 1 R2 and Het have the same meanings as defined in the general formula The reaction can be conducted by performing the reaction 20 in the presence or absence of a base such as n-butyl lithium, lithium or diisopropylamide in a solvent which is not involved in a solvent at a temperature of -100 to 50'C, preferably -80 to O:C for 10 minutes to 12 hours, preferably 30 minutes to 6 hours, and removing the protective groups, if necessary.
25 The above compound of the formula (XI) can be prepared according to a well-known method, for example, described in Comprehensive Heterocyclic Chemistry, Vol. 6, 293.
7 8 9 In the case of the compound wherein R R or R represent an amidino group: This compound can be prepared by reacting the compound represented by the formula wherein R 7 R and R represent hydrogen, with the compound represented by the formula (XII):
CH
3 N 2
(XII)
in the presence or absence of a base in an inert solvent at a temperature of -30 to 100'C, preferably -20 to 80:C for 30 minutes to 48 hours, preferably 1 to 10 hours, and removing the protective groups, if necessary.
In the case of the compound wherein Y represents the group where Z represents the group -(CH2)m-CO-: This compound can be prepared by oxidizing the hydroxyl group of the corresponding compound wherein Y represents the group where Z represents the group -(CH,)m-CHR where R 6 represents hydroxyl, with an appropriate oxidizing agent such as manganese dioxide, pyridinium chlorochromate or pyridinium dichromate.
In this context, it will be appreciated by one skilled in the art that in the above processes, the sequences of synthesis are determined so that no side reaction will occur in functional groups which are not involved in the reaction and that the functional groups may be protected with protective groups to avoid any,undesirable reactions.
20 Use of the compound/pharmaceutical composition The compound according to the present invention inhibits the aggregation of platelets by inhibiting the binding of platelet membrane protein GPIIb/IIIa and fibrinogen. Thus, the compound according to the present invention and a 25 pharmacologically acceptable salt thereof are effective in the treatment and prophylaxis of thrombotic.disorders caused by the aggregation of platelets, particularly cerebral infarction, myocardial infarction, angina pectoris or peripheral arterioocclusion.
A pharmaceutical composition comprising the compound according to the present invention or a pharmacologically acceptable salt thereof as effective ingredients can be administered human and non-human animal subjects through any one of routes such as oral or parenteral routes such as intravenous injection, intramuscular injection, subcutaneous administration, rectal administration or percutaneous administration.
Therefore, the pharmaceutical composition comprising the compound according to the present invention is processed into suitable dosage forms depending on dosage routes, and can be specifically formed into preparations mainly including injections such as intravenous injection or intramuscular injection, oral preparations such as capsule, tablet, granule, powder, pill, grains or troche, rectal preparations, oily suppositories or aqueous suppositories.
These preparations can be prepared in the usual manners with conventional additives such as an excipient, a filler, a binder, a humidifier, a disintegrating agent, a surface active agent, a lubricant, a dispersant, a buffer, a preservative, a dissolution aid, an antiseptic agent, a flavoring agent, an analgesic agent or a stabilizer. The aforementioned acceptable and non-toxic additives include, for example, lactose, fructose, glucose, starch, gelatin, magnesium carbonate, synthetic magnesium silicate, talc, magnesium stearate, methyl cellulose or a salt thereof, gum arabic, polyethylene glycol, syrup, vaseline, ethanol, propylene glycol, citric acid, sodium chloride, sodium sulfite and sodium phosphate.
The compound according to the present invention in a S pharmaceutical composition is contained in amounts depending on its dosage forms, which generally range from about 1 to 70% by weight, preferably from about 5 to 50% by weight of the total composition.
The dose is appropriately determined in consideration of the use, and the age, sex and severity of a patient. The dose is generally in the range from about 0.1 to 1,000 mg, preferably from 1 to 200 mg per day to an adult patient for the purpose of the treatment of thrombotic disorders. The dose may be administered in one or more portions per day.
Examples The present invention is now described in detail with reference to the following examples, but it should not be construed that the invention be limited thereto.
Example 1 [[4-[[[trans-4-(aminomethyl)cyclohexyl]carbonylamino]acetyl]-ophenylene]dioxy]diacetic acid trifluoroacetate Trans-4-(t-butyloxycarbonylaminomethyl)cyclohexanecarboxylic acid To the solution of trans-4-(aminomethyl) cyclohexanecarboxylic acid (10 g, 63.6 mmole) in the mixture of 100 ml of water and 20 ml of dimethylformamide were added 9 ml of triethylamine and 22.6 ml of di-t-butyl dicarbonate, and the mixture was stirred at room temperature for 4 hours. To the reaction mixture was added 300 ml of ethyl acetate, and the resulting mixture was acidified with IN hydrochloric acid. The organic layer was dried over anhydrous magnesium sulfate. The inorganic salt was removed by filtration, and the filtrate was 15 concentrated under reduced pressure. Solids thus obtained were washed with n-hexane to give the title compound as colorless crystals (yield, 1 H-NMR (CDC13) 6: 0.95 1.02 2H), 1.37 1.44 12H), 1.82 2H), 2.04 2H), 2.22 2.30 1H), 2.98 2H).
20 Dimethyl [[4-(aminoacetyl)-o-phenylene]dioxy]diacetate trifluoroacetate b-l) To the solution of a-amino-3,4-dihydroxyacetophenone S. g, 18 mmole) in 30 ml of dimethylformamide were added 4.3 ml of di-t-butyl dicarbonate and a catalytic amount of 4- 25 dimethylaminopyridine, and the mixture was stirred at room temperature for 3 hours. After the reaction mixture was concentrated under reduced pressure, the rusulting oily product was dissolved in ethyl acetate, washed with an aqueous sodium hydrogen carbonate solution and then with water, and dried over anhydrous magnesium sulfate. The inorganic salt was removed by filtration. The filtrate was concentrated under reduced pressure.
The solid product thus obtained was purified by column chromatography on silica gel to give 1.3 g of a-(tbutyloxycarbonyl)amino-3, 4-dihydroxyacetophenone as an orange solid (yield, 28%) 1H-NMR (CDC1 3 6: 1.47 9H), 4.54 2H), 6.90 1H), 7.41 1H), 7.45 1H).
b-2) To the solution of the compound obtained in b-1) (1.0 g, 3.7 mmole) in acetone were added 1.1 g of potassium carbonate and ml of methyl bromoacetate, and the mixture was stirred at room temperature overnight. After the reaction mixture was concentrated under reduced pressure, the solid product thus obtained was dissolved in 200 ml of ethyl acetate, washed with water and dried over anhydrous magnesium sulfate. The inorganic salt was removed by filtration, and the filtrate was concentrated under reduced pressure. The resulting oily product was purified by column chromatography on silica gel to give 1.5 g of dimethyl [[4-[(t-butyl-oxycarbonylamino)acetyl]-o-pheylene] dioxy]diacetate as an orange oily product (yield, 100%).
1 H-NMR (CDC1 3 6: 1.47 9H), 3.80 3H), 3.81 3H), 4.58 2H), 4.78 2H), 4.81 2H), 6.86 1H), 7.51 1H), 15 7.58 1H).
b-3) To the solution of the compound prepared in b-2) (1.5 g, 3.7 mmole) in 15 ml of dichloromethane were added 4 ml of anisole and 5.6 ml of trifluoroacetic acid, and the mixture was stirred at room temperature for 1 hour. After the reaction 20 mixture was concentrated under reduced pressure, the resulting solids were washed with ether to give 1.2 g of the title compound as pale yellow powder (yield, 79%).
H-NMR (.CD 3 OD) 6: 3.78 3H), 3.79 2H), 4.52 2H), 4.85 2H), 4.91 2H), 7.07 2H), 7.60 1H), 7.70 (dd, 1H).
25 To the solution of 877 mg (3.41mmol) of the compound prepared in (3.41 mmole) in the mixture of 6.8 ml of dimethylformamide and 1.4 ml of pyridine were added 874 mg of N,N'-disuccinimidyl carbonate and a catalytic amount of 4dimethylaminopyridine, and the resulting mixture was stirred at room temperature for 3 hours, whereafter 1.45 g of the compound prepared in and 0.59 ml of N,N-diisopropylethylamine were added. The mixture was further stirred at room temperature overnight. After the reaction mixture was concentrated under reduced pressure, the resulting solid product was purified by column chromatography on silica gel. The solid product thus obtained was washed with ether to give 1.1 g of dimethyl [[[trans-4-(t-butyloxycarbonylaminomethyl)cyclohexyl] carbonylamino]acetyl]-o-phenylene]dioxy]diacetate as pale yellow crystals (yield, 59%).
H-NMR (CDC1 3 8: 0.90 1.10 2H), 1.44 1.56 12H), 1.86 2H), 1.98 2H), 2.14 2.22 1H), 3.05 3.10 (m, 2H), 3.81 6H), 4.76 2H), 4.78 2H), 4.81 2H), 6.55 1H), 6.87 1H), 7.51 1H), 7.61 (dd, 1H).
To the suspension of 300 mg (0.55 mmole) of the compound prepared in in 3 ml of methanol was added 3 ml of IN potassium hydroxide, and the mixture was stirred under icecooling for 15 minutes. .After methanol was evaporated under reduced pressure, ethyl acetate and water were added to the residue, and the mixture was acidified with IN hydrochloric acid.
After the organic layer was dried over magnesium sulfate, the 0"0 inorganic salt was removed by filtration. The filtrate was 15 concentrated under reduced pressure. Solids thus obtained were S washed with ether to give 243 mg of butyloxycarbonylaminomethyl)cyclohexyl]carbonylamino]acetyl]-ophenyleneldioxy]diacetic acid as pale yellow powder.
H-NMR (CDC13) 6: 0.98 1.04 2H), 1.44 1.54 12H), 20 1.84 1.98 4H), 2.17 2.20 1H), 2.97 2H), 4.65 (s, 2H), 4.75 2H), 4.77 2H), 6.91 1H), 7.51 1H), 7.61 (dd, 1H).
,To the solution of 193 mg (0.37 mmole) of the compound prepared in in 2 ml of dichloromethane were added 0.6 ml of 0* 25 trifluoroacetic acid and 0.4 ml of anisole, and the mixture was stirred at room temperature for 1.5 hours. After the reaction mixture was concentrated under reduced pressure, the solid product thus obtained was washed with ether to give 190 mg of the title compound as yellow powder (yield, 96%).
1H-NMR (CD 3 0D) 6: 1.09 1.20 2H), 1.49 1.70 3H), 1.89 2.05 4H), 2.30 2.38 1H), 2.79 2H), 4.63 2H), 4.78 2H), 4.83 2H), 7.05 1H), 7.58 1H), 7.70 (dd, 1H).
FDMS 423 (M 1).
Example 2 [trans-4-(methylaminomethyl )cyclohexyl] carbonylamino]acetyl]-o-phenylene]dioxy]diacetic acid trifluoroacetate To the solution of 516 mg (2 mmole) of the compound prepared in Example 1(a) in 10 ml of dimethylformamide were added 1 ml of methyl iodide and 1.85 g of silver oxide, and the mixture was stirred at 45-C for 7 hours and then at room temperature overnight. After insolubles were removed by filtration, chloroform was added. The mixture was washed thrice with water and dried over anhydrous magnesium sulfate. The inorganic salt was removed by filtration, and the filtrate was concentrated under reduced pressure. The oily product thus obtained was purified by column chromatography on silica gel to give 114 mg of methyl trans-4-(N-t-butyloxycarbonyl-Nmethyl)aminomethylcyclohexanecarboxylate as a colorless oil 15 (yield, IH-NMR (CDC1 3 6: 0.95 1.03 2H), 1.40 1.50 12H), 1.75 2H), 2.01 2H), 2.25 1H), 2.85 2H), 3.06 3H), 3.67 3H).
To the solution of 352 mg (1.23 mmole) of the compound 20 prepared in in 6 ml of methanol was added 6 ml of IN sodium hydroxide, and the mixture was stirred at room temperature for 1.5 hours. After the solution was concentrated under reduced pressure, chloroform and water were added to the residue. After the mixture was acidified with IN hydrochloric acid, the organic 25 layer was dried over anhydrous magnesium sulfate. The inorganic salt was removed by filtration. The filtrate was then concentrated under reduced pressure. The oily product thus obtained was purified by column chromatography on silica gel to give 300 mg of trans-4-(N-t-butyloxycarbonyl-Nmethyl)aminomethylcyclohexanecarboxylic acid as a colorless oily material (yield, 1H-NMR (CDC1 3 6: 0.95 1.03 2H), 1.40 1.48 12H), 1.77 2H), 2.04 2H), 2.27 1H), 2.85 2H), 3.06 3H).
According to the method of Example 211 mg of dimethyl [trans- 4- [(N-methyl-N-tbutyloxycarbonyl)aminomethyl ]cyclohexyl]carbonylamino]acetyl]-ophenylene]dioxy]diacetate was obtained from 135 mg of the compound prepared in Example l(b) (0.5 mmole) and 210 mg of the compound prepared in Example l(b) (yield, 1H-NMR (CDC13) 6: 1.00 2H), 1.45 1.52 12H), 1.78 (d, 2H), 1.98 2H), 2.19 1H), 2.85 2H), 3.07 (bs, 3H), 3.81 6H), 4.68 2H), 4.79 2H), 4.82 2H), 6.54 (bs, 1H), 6.87 1H), 7.51 1H), 7.61 (dd, 1H).
According to the method of Example 123 mg of [trans-4-[ (N-methyl-N-t-butyloxycarbonyl)aminomethyl]cyclohexyl]carbonylamino]acetyl]-ophenylene]dioxy]diacetic acid was obtained from 210 mg (0.37 mmole) of the compound prepared in (yield, 62%).
1 H-NMR (CDCl 3 6: 1.02 2H), 1.45 1.65 12H), 1.78 (m, 2H), 1.97 2H), 2.22 2H), 2.84 2H), 3.07 (bs, 3H), 4.65 2H), 4.74 2H), 4.77 2H), 6.91 1H), 7.52 (d, 15 1H), 7.63 (dd, 1H).
According to the method of Example 89 mg of the title compound was obtained from 100 mg (0.2 mmole) of the compound prepared in (yield, 87%).
1 H-NMR (CD3OD) 6: 1.12 2H), 1.50 1.70 3H), 1.90 2.00 4H), 2.33 1H), 2.70 3H), 2.86 2H), 4.63 2H), 4.78 2H), 4.83 2H), 7.03 1H), 7.58 1H), 7.68 (dd, 1H).
Example 3 25 [[4-[[[trans-4-(guanidinomethyl)cyclohexyl]carbonylamino]acetyl]o-phenylene]dioxy]diacetic acid To the solution of 70 mg (0.13 mmole) of the compound in Example 1 in 0.1 ml of concentrated aqueous ammonia was added mg of methyl isothiourea sulfate, and the mixture was stirred at room temperature overnight. After methanol was added to the reaction mixture, crystals were collected by filtration and dried to give 39 mg of the title compound (yield, 64%).
H-NMR 6: 1.05 1.20 2H), 1.42 1.65 3H), 1.88 2.00 4H), 2.35 2.42 1H), 3.08 2H), 4.67 2H), 4.71 2H), 4.73 2H), 7.02 1H), 7.45 1H), 7.73 (d, 1H).
FDMS 465 (M 1).
Example 4 2-(4-aminomethylcyclohexyl)-5-(3,4-dicarboxymethoxy)phenyloxazole To 150 mg (0.28 mmole) of the compound of Example 1 was added 0.3 ml of concentrated sulfuric acid, and the mixture was stirred at room temperature for 5 minutes. After a small amount of water was added to the reaction mixture, the resulting mixture was purified on HP-20 and then lyophilized to give 33 mg of the title compound as colorless powder (yield, 29%).
FDMS 405 (M 1).
Example S[ [4-[[[trans-4-(acetylaminomethyl)cyclohexyl] 15 carbonylamino]acetyl]-o-phenylene]dioxy]diacetic acid disodium salt To the solution of 550 mg (1 mmole) of the compound prepared in Example l(c) in 5.5 ml of dichloromethane were added 1.1 ml of anisole and 2 ml of trifluoroacetic acid, and the S* mixture was stirred at room temperature. The reaction mixture was concentrated under reduced pressure and then dried thoroughly to give a quantitative amount of dimethyl [[4-[[[trans-4- (amino..methyl)cyclohexyl] carbonylamino] acetyl] -ophenylene]dioxy]diacetate trifluoroacetic acid salt. The oily 25 product unpurified was used directly for the following reaction.
To 205 mg (0.36 mmole) of the compound prepared in (a) were added 2 ml of pyridine and 1 ml of acetic anhydride, and the mixture was stirred at room temperature overnight. After ethyl acetate was added to the reaction mixture, the mixture was washed with water and then dried over anhydrous magnesium sulfate. The inorganic salt was removed by filtration. The filtrate was concentrated under reduced pressure. The oily product thus obtained was purified by column chromatography on silica gel to give 92 mg of dimethyl [[4-[[[trans-4- (acetylaminomethyl)cyclohexyl]carbonylamino]acetyl]-ophenylene]dioxy]diacetate as pale yellow crystals (yield, 51%).
1H-NMR (CDC13) 6: 1.00 1.10 2H), 1.47 1.55 3H), 1.86 2H), 1.97 2.03 5H), 3.13 2H), 3.81 6H), 4.68 2H), 4.79 2H), 4.81 2H), 5.51 (bs, 1H), 6.56 (bs, 1H), 6.87 1H), 7.51 1H), 7.62 (dd, 1H).
To the suspension of 92 mg (0.19 mmole) of the compound prepared in in 2 ml of methanol was added 1 ml of IN sodium hydroxide, and the mixture was stirred at a temperature from under ice-cooling to room temperature for 1 hour. After the reaction mixture was concentrated under reduced pressure, the residue thus obtained was purified on HP-20 and then lyophilized to give 44 mg of the title compound (yield, 46%).
1H-NMR (D20) 6: 1.00 1.10 2H), 1.38 1.50 3H), 1.80 1.85 2H), 1.90 1.95 2H), 2.00 3H), 2.30 2.45 (m, 1H), 3.06 2H), 4.62 2H), 4.70 2H), 6.97 1H), 7.41 1H), 7.70 1H).
Example 6 [[[trans-4- (p-amidinobenzoyl)aminomethyl] cyclohexyl]carbonylamino]acetyl]-o-phenylene]dioxy]diacetic acid disodium salt 20 To the solution of 470 mg (0.83 mmole) of the compound prepared in Example 5 in 5 ml of dimethylformamide were added 0.23 ml of triethylamine and 138 mg of 4-cyanobenzoyl chloride, and the mixture was stirred at room temperature for 1 hour. After the reaction mixture was concentrated under reduced pressure, the 25 residue thus obtained was dissolved in chloroform, washed with water and dried over anhydrous magnesium sulfate. After the inorganic salt was removed by filtration, the filtrate was concentrated under reduced pressure. The solid product thus obtained was washed with ether to give 300 mg of dimethyl [[[trans-4-[(p-cyanobenzoyl)aminomethyl]cyclohexyl] carbonylamino]acetyl]-o-phenylene]dioxy]diacetate (yield, 62%).
1H-NMR (CDC1 3 6: 1.05 1.15 2H), 1.50 1.62 3H), 1.88 2.05 4H), 2.15 2.22 1H), 3.34 3.37 2H), 3.81 6H), 4.67 2H), 4.78 2H), 4.81 2H), 6.24 (bs, 1H), 6.55 (bs, 1H), 6.87 1H), 7.51 1H), 7.60 1H), 7.74 (d, 2H), 7.87 2H).
To the solution of 350 mg (0.6 mmole) of the compound prepared in in the mixture of 15 ml of pyridine and 1 ml of triethylamine, and hydrogen sulfide gas was streamed into the mixture under ice-cooling for 30 minutes before the resulting mixture was stirred at room temperature overnight. The reaction mixture was diluted with ethyl acetate, washed with a 5% sodium hydrogen carbonate solution followed by a 1M potassium hydrogen sulfate solution, and dried over anhydrous magnesium sulfate.
After the inorganic salt was removed by filtration, the filtrate was concentrated under reduced pressure. The residue thus obtained was purified by column chromatography on silica gel to give 288 mg of dimethyl [[4-[[[trans-4-[(p-thiocarbamoylbenzoyl) aminomethyl]cyclohexyl]carbonylamino]acetyl]-ophenylene]dioxy]diacetate as a yellow foam product (yield, 78%).
S.
1 IH-NMR (CDC1 3 6: 1.05 1.15 2H), 1.48 1.70 3H), 1.86 15 2.01 4H), 2.18 2.25 1H), 3.34 2H), 3.81 6H), *4.66 2H), 4.79 2H), 6.54 1H), 6.64 1H), 6.87 (d, 1H), 7.52 1H), 7.61 (dd, 1H), 7.77 2H), 7.90 2H).
To the solution of 288 mg (0.47 mmole) of the compound prepared in in 15 ml of acetone was added 3 ml of methyl e iodide, and the mixture was refluxed for 1 hour. After the reaction mixture was concentrated under reduced pressure, the residue thus obtained was purified by column chromatography on silica gel to give 201 mg of dimethyl methylthio-l-imino)methyl]benzoyl]aminomethyl]cyclohexyl] carbonylamino]acetyl]-o-phenylene]dioxy]diacetate as pale yellow crystals (yield, 68%).
1H-NMR (CDC1 3 6: 1.09 1.20 2H), 1.50 1.60 3H), 1.92 2.03 4H), 2.45 3H), 3.35 2H), 3.81 6H), 4.67 2H), 4.79 2H), 4.82(s, 2H), 6.35 1H), 6.60 1H), 6.87 1H), 7.51 1H), 7.61 1H), 7.82 4H).
To the solution of 150 mg (0.24 mmole) of the compound prepared in in 3 ml of methanol was added 31 mg of ammonium acetate, and the mixture was refluxed for 5.5 hours. Deposited solids were collected by filtration to give 44 mg of dimethyl [4-[[[trans-4-[(p-amidinobenzoyl)aminomethyl] cyclohexyl]carbonylamino]acetyl]-o-phenylene]dioxy]diacetate (yield, 31%).
1H-NMR (CDC1 3 6: 1.05 1.14 2H), 1.49 1.68 3H), 1.91 2.01 4H), 2.20 2.38 1H), 3.32 2H), 3.82 6H), 4.66 2H), 4.79 2H), 4.82(s, 2H), 6.87 1H), 7.52 (d, 1H), 7.63 (dd, 2H), 7.75 2H), 7.91 2H).
To the solution of 40 mg (0.07 mmole) of the compound prepared in in 2 ml of methanol was added 1 ml of IN sodium hydroxide, and the mixture was stirred at a temperature from icecooling to room temperature for 6 hours. After the reaction mixture was concentrated under reduced pressure, the residue thus obtained was purified on HP-20 and then lyophilized to give 22 mg of the title compound.
1 H-NMR (D 2 0) 6: 1.10 1.20 2H),-1.47 1.52 2H), 1.69 1H), 1.92 2.00 2.40 1H), 3.32 2H), 4.64 2H), 4.68 2H), 4.72 2H), 7.00 1H), 7.43 1H), 7.72 1H), 7.88 7.94 4H).
Example 7 [2-[trans-4-(aminomethyl)cyclohexyl]carbonylamino]ethyl]-ophenylene]dioxy]diacetic acid trifluoroacetate 20 The title compound as a colorless crystal was prepared from 130 mg of the compound obtained in Example 1 and 220 mg of diethyl [[4-(2-aminoethyl)-o-phenylene]dioxy]diacetate according to the process described in Example 1 1H-NMR (CD30D) 6: 1.00 1.08 2H), 1.42 1.65 3H), 1.80 25 1.88 4H), 2.07 2.15 1H), 2.71 2H), 2.77 2H), 3.31 2H), 3.36 2H), 4.68 2H), 4.71 2H), 6.78 (dd, 1H), 6.85 1H), 6.90 1H).
Example 8 [[trans-4-(aminomethyl)cyclohexyl]carbonyl] (N-methyl )amino] acetyl]-o-phenylene]dioxy]diacetic acid trifluoroacetate H-NMR (CD 3 OD) 6: 1.12 3H), 1.45 1.70 3H), 1.81 (d, 1H), 1.96 3H), 2.72, 2.78 (2d, 2H), 2.94, 3.16 (2s, 3H), 4.77, 4.79 (2s, 2H), 4.82, 5.01 (2s, 2H), 4.83, 4.84 (2s, 2H), 7.02, 7.04 1H), 7.56, 7.60 (2d 1H), 7.68, 7.72 (2dd, 1H).
SIMS 437 1).
Example 9 [[4-[[N-[3-(piperidin-4-yl)propionyl]-N-methylamino]acetyl]-ophenylene]dioxy]diacetic acid trifluoroacetate Di-t-butyl [[4-[[N-[3-(l-t-butyloxycarbonylpiperidin-4yl)propionyl]-N-methylamino]acetyl]-o-phenylene]dioxy]diacetate 3-(l-t-butyloxycarbonylpiperidin-4-yl)propionic acid (257 mg), di-t-butyl [[4-[(N-methylamino)acetyl]-ophenylene]dioxy]diacetate hydrochloride (446 mg), N-methylmorpholine (101 mg), benzotriazol-1-yloxytris( dimethylamino)phosphonium hexafluorophosphate (BOP reagent, 442 mg), and a catalytic amount of 4-dimethylaminopyridine were dissolved in 5 ml of dimethylformamide, and the mixture was stirred at room temperature for 3 hours. After ethyl acetate (100 ml) was added to the reaction mixture, the resulting mixture was washed with 15 water. The solvents were removed under reduced pressure. The residual mixture was purified by column chromatography on silica Sgel to give 285 mg of the title compound from the fraction eluted with n-hexan ethyl acetate 3 1.
1 H-NMR (CDClI) 6: 1.04 1.15 2H), 1.38 1.54 28H), 1.54 20 1.75 4H), 2.35 2.48 2H), 2.58 2.75 2H), 3.08 3H), 3.98 4.29 2H), 4.62 2H), 4.66 2H), 4.77 2H), 6.82 J 8 Hz, 1H), 7.47 1H), 7.60-(d, J 8 Hz, 1H).
*i SIMS 649 (M 1).
25 The solution of 250 mg of the compound prepared in in 2 ml of trifluoroacetic acid was stirred at room temperature for 3 hours. After the solvent was evaporated under reduced pressure, ether was added and deposited crystals were collected by filtration to give 168 mg of the title compound.
IH-NMR (DI0) 6: 1.22 1.45 2H), 1.48 1.68 3H), 1.77 2.02 2H), 2.40 2.60 2H), 2.85 3.02 2H), 2.96, 3.13 3H), 3.29 3.45 2H), 4.82 5.06 6H), 7.02 7.08 1H), 7.45 7.48 1H), 7.66 7.75 1H).
SIMS 437 1).
Example [[4-[[N-[3-(piperidin-4-yl)propionyl]amino]acetyl]-o- 27 phenylenel dioxy] diacetic acid trifluoroaceetate Di-t-butyl N-13-(1-t-butyloxycarbonylpiperidin-4yl )propionyllaminollacetyl] -o-phenylene]dioxy]diacetate 3- -t-butyloxycarbonylpiperidin-4-yl )propionic acid (423 mg), di-t-buty [[4-(aminoacetyl )-o-phenyleneldioxy]diacetate (650 N-methylmorpholine (167 mg), benzotriazol-lyloxytris( dimethylainino )phosphonium hexafluorophosphate (BOP reagent, 730 mg) and a catalytic amount of 4diiethylaminopyridine were dissolved in 10 ml of dimethylfornaiide and treated -in the same manner as described in Example 9 to give the title compound (485 mg) from the fraction eluted with n-hexane :ethyl1 acetate 2 :1.
'H-NMR (CDCl 3 6: 1.05 1.18 (in, 2H), 1.45 9H), 1.48 (s, 9H), 1.50 9H), 1.58 1.70 (in, 5H), 2.30 2.37 (in, 2H), 2.62 2.72 (in, 2H), 4.02 4.17 (in, 2H), 4.66 2H), 4.69 4H), :*6.53 (brs, 1H), 6.83 J 9 Hz, 1H), 7.48 1H), 7.60 (d, J 9 Hz, 1H).
FDMS 634 The compound prepared in (480 mg) was dissolved in ml of trifluoroacetic acid and treated in the same manner as described in Example 9 to give the title compound (380 mg).
IH-NMR 1.32 1.44 (mn, 2H), 1.53 -1.67 (in, 3H), 1.88 1.98 2H), 2.34 2.45 (in, 2H), 2.90 -3.02 (in, 2H), 3.34 3.47 (mn, 2H), 4.65 2H), 4.84 2H), 4.88 2H), 7.04 (d, J 9 Hz, 1H), 7.45 1H), 7.66 J 9 Hz, 1H).
SIMS 423 1).
Example 11 [[4-[[(piperidin-4-yl)acetylanino]acetyl]o-pheylele] dioxy]diacetic acid trifluoroacetate The title compound was prepared in the same manner as in Example 9.
Di-t-butyl (-t-butyloxycarbonylpiperidin-4yl )acetylainino] acetyl-o-phenylene] dioxy] diacetate IH-NMR (CDCl 3 6: 1.25 (in, 2H), 1.45 9H), 1.48 9H), 1.50 9H), 1.70 (in, 2H), 2.00 (in, 1H), 2.25 (in, 2H), 2.72 (mn, 2H), 4.09 (brs, 2H), 4.66 2H), 4.69 2H), 4.70 2H), 6.56 (brs, 1H), 6.83 1H), 7.47 lH), 7.59 (dd, 1H).
FDMS 620 (Me).
[[4-[[(piperidin-4- yl)acetylamino]acet yl]-ophenylene]dioxy]diacetic acid trifluoroacetate IH-NMR (D 2 0) 8: 1.50 2H), 1.95 2.15 3H), 2.39 2H), 3.05 2H), 3.45 2H), 4.70 2H), 4.83 2H), 4.86 (s, 2H), 7.06 1H), 7.48 1H), 7.70 1H).
Example 12 [[4-[[[4-(piperi din-4-yl)butyryl]amino~ aetl]o phenylene]dioxy diacetic acid trifliroroacetate The title compound was prepared in the same manner as in *5 S S Example 9.
Di-t-butyl [[4-[[[4-(l-t-butyloxycarbonylpiperidin- 4 yl)butyryllamino]acetyl-o-phenylene]dioxy]diacetate 1 H-NMR (CDCl 3 8: 1.04 2H), 1.28 2H), 1.45 9H), 1.
9H), 1.50 2H), 1.60 1.72 5H), 2.30 2H), 2.
2H), 4.07 (brs, 1H), 4.66 2H), 4.69 2H), 4.70 2H), 6.54 (brs, 1H), 6.83 1H), 7.58 1H), 7.60 1H) FDMS 649 1).
[[4-[[[4-(piperidin-4-yl)butyryl]aminO]acetyl]-ophenylene]dioxy]diacetic acid trifluoroacetate 'H-NMR (D 2 0) 8: 1.25 1.40 4H), 1.52 1.68 3H), 1.
2H), 2.38 2H), 2.96 2H), 3.40 2H), 4.66 2 4.85 2H), 4.89 2H), 7.06 1H), 7.48 1H), 7.70 (C 1H).
48 67
S,
9 1), Id, Example 13 [[4-[[[(4,5,6,7-tetrahydrothieno[3,2-cpyridin-2-yl)carbonyl] aminolacetyl]-o-phenyleneldioxyldiacetic acid trifluoroacetate The title compound was prepared in the same manner as in Example 9.
Di-t-butyl [[4-[[[(5-t-butyloxycarbonyl-4,5, 6 7 tetrahydrothieno[3,2-c]pyridin-2-yl)carbonyl]aino]acetyl-ophenylene]dioxy]diacetate 1H-NMR (CDCl 3 6: 1.48 1.58 27H), 2.87 (brs, 2H), 3.73 (brs, 2H), 4.49 2H), 4.67 2H), 4.70 2H), 4.84 (d, 2H), 6.85 1H), 7.08 (brs, 1H), 7.33 1H), 7.50 1H), 7.63 1H).
[[4-[[[(4,5,6,7-tetrahydrothieno[3,2-c]pyridin-2yl)carbonyl]amino]acetyl]-o-phenylene]dioxy]diacetic acid trifluoroacetate IH-NMR (DMSO-d 6 6: 3.08 2H), 3.37 3.61 2H), 4.24 (s, 2H), 4.70 2H), 4.79 2H), 4.85 2H), 7.02 1H), 7.42 1H), 7.60 1H), 7.70 1H), 8.82 (brs, 1H), 9.09 (brs, 1H).
Example 14 piperidin-4-yl )carbonyl ]amino]acetyl]-ophenylene]dioxy]diacetic acid trifluoroacetate The title compound was prepared in the same manner as in Example 9.
Df-t-butyl [[4-[[(l-t-butyloxycarbonylpiperidin-4yl)carbonylamino]acetyl-o-phenylene]dioxy]diacetate 1H-NMR (CDC1 3 6: 1.48 27H), 1.72 2H), 1.87 2H), 2.38 1H), 2.77 2H), 4.18 2H), 4.66 2H), 4.67 2H), 4.69 2H), 6.60 (brs, 1H), 6.83 1H), 7.47 1H), 7.58 (dd, 1H).
FDMS 606 25 [[4-[[(piperidin-4-yl)carbonyl]amino]acetyl]-ophenylene]dioxy]diacetic acid trifluoroacetate 1H-NMR (D20) 5: 1.91 2H), 2.15 2H), 2.80 1H), 3.14 2H), 3.53 2H), 4.70 2H), 4.86 2H), 4.90 2H), 7.06 1H), 7.48 1H), 7.69 1H).
FDMS 395 1).
Example [[4-[2-[3-(piperidin-4-yl)propionyl]aminoethyl]-ophenylene]dioxy]diacetic acid trifluoroacetate The title compound was prepared in the same manner as in Example 9 except that the following process was further carried out.
Diethyl [[4-[2-[3-(1-t-butyloxycarbonylpiperidin-4yl)propionyl]aminoethyl]-o-phenylene]dioxy]diacetate 'H-NMR (CDCl 3 8: 1.15 2H), 1.28 6H), 1.45 9H), 1.56 1.70 3H), 2.15 2H), 2.60 2.78 4H), 3.46 2H), 4.07 (brs, 2H), 4.25 4H), 4.69 2H), 4.71 2H), 5.45 (brs, 1H), 6.74 6.83 3H).
[[4-[2-[3-(-t-butyloxycarboylpiperidin-4yl)propionyl]aminoethyl]-o-phenylene]dioxy]diacetic acid 'H-NMR (CDCl 3 8: 1.05 (in; 2H), 1.44 9H), 1.58 3H), 2.15 2H), 2.60 2.70 4H), 3.42 4H), 3.96 4.30 4H), 4.67 2H), 4.68 2H), 6.68 6-.80 3H).
The compound obtained in the above process (150 mg) was hydrolyzed with 1.3 ml of 1N sodium hydroxide to give 121 mg of the title compound.
4-[2-[3-(piperidin-4-yl)propionyl aminoethyl]-ophenylene~dioxy]diacetic acid trifluoroacetate 1 H-NMR (C6C1 3 8: 1.25 1.40 3H), 1.49 2H), 1.82 (i, 2H), 2.17 2H), 2.74 2H), 2.88 2H), 3.42 2H), 4.69 2H), 4.72 2H), 6.83 6.90 3H).
Example 16 [[4-[2"-[(4,5,6,7-tetrahydrothieno[3,2-c]pyridin-2yl)carbonyl]aminoethyl]-o-phenylene]dioxy]diacetic acid 25 trifluoroacetate The title compound was prepared in the saie manner as in Example Diethyl [[4-[2-[(5-t-butyloxycarbonyl-4,5,6,7-tetrahydro thieno[3,2-c]pyridin-2-yl)carbonyljaminoethyl]-ophenylene]dioxyldiacetate IH-NMR (CDCl 3 6: 1.25 1.30 6H), 1.48 9H), 2.79 2.82 4H), 3.58 2H), 3.70 2H), 4.18 4.27 4H), 4.42 2H), 4.68 4H), 6.75 6.83 3H), 7.17 1H).
[[4-[2-[(4,5,6,7-tetrahydrothieno[3,2-c]pyridin-2yl )carbonyl]aminoethyl]-o-phenylene]dioxy]diacetic acid trifluoroacetate 1H-NMR (DMSO-d 6 6: 2.73 2H), 3.04 (brs, 2H), 3.36 3.39 (m, 4H), 4.14 (brs, 2H), 4.40 (brs, 2H), 4.47 (brs, 2H), 6.75 6.83 3H), 7.54 1H), 8.59 (brs, 1H).
Example 17 [[4-[[(l-amidinopiperidin-4-yl)carbonylamino]acetyl]-ophenylene]dioxy]diacetic acid The compound prepared in Example 14 (50 mg) was dissolved in concentrated aqueous ammonia, methyl isothiourea sulfate (16 mg) was added to the solution. The mixture was stirred at room temperature overnight. After methanol was added to the reaction mixture, the resulting mixture was stirred at room temperature for a while, filtered under reduced pressure to give 24 mg of the title compound as pale yellow powder (yield, 15 H-NMR (D 2 0) 6: 1.68 2H), 1.96 2H), 2.75 1H), 3.19 2H), 3.87 2H), 4.61 2H), 4.65 2H), 4.68 2H), 6.95 1H), 7.37 1H), 7.67 1H).
FDMS 437 1).
Referenctial Example 1 S: 5-t-butoxycarbonyl-4,5,6,7-tetrahydrothieno[3,2-c]pyridin-2carboxylic acid To the solution of 4,5,6,7-tetrahydrothieno[3,2c]pyridin-2-carboxylic acid (7.6 g) in DMF (75 ml) was added di- 25 t-butyl dicarbonate (9.6 ml), and triethylamine (5.8 ml) was titrated to the mixture. After stirrin. at room temperature for 3 hours and the addition of water, the reaction mixture was acidified with 1N hydrochloric acid and extracted with ethyl acetate. After the organic layer was washed with water, dried over anhydrous magnesium sulfate, the solvent was evaporated.
After n-hexan and a small amount of ether were added to the residue, crystals deposited were collected by filtration to give 9.36 g of the title compound (yield, H-NMR (CDCl 3 6: 1.49 9H), 2.90 (brs, 2H), 3.74 (brs, 2H), 4.51 (brs, 2H), 7.56 1H).
SIMS 284 (M 1).
Referential Example 2 5-t-butoxycarbonyl-3-methyl-4,5,6,7-tetrahydrothieno[3,2c]pyridin-2-carboxylic acid The compound prepared in Referential Example 1 (300 mg) was dissolved in THF (5 ml), and the mixture was cooled to -78C.
After n-butyl lithium (15% hexane solution, 1.5 ml) was added, the mixture was stirred at -78'C for 1 hour. Methyl iodide (0.16 ml) was added to the mixture that was then further stirred with heating to room temperature for 1.5 hours. After ethyl acetate and water were added to the reaction mixture, the resulting mixture was acidified with IN hydrochloric acid. The organic layer was washed with water and dried over anhydrous magnesium sulfate. The solvent was removed by distillation. The residue was purified by column chromatography on silica gel to give 153 mg 15 of the title compound from the fraction eluted with chloroform methanol 20 1.
'H-NMR (CDC1 3 6: 1.50 9H), 2.43 3H), 2.85 (brs, 2H), 3.72 (brs, 2H), 4.38 (brs, 2H).
SIMS 298 (M 1).
S Referential Example 3 5-t-butoxycarbonyl-3-benzyl-4,5,6, 7-tetrahydrothieno[3,2c]pyridin-2-carboxylic acid The title compound was prepared in the same manner as in Referential Example 2 except that methyl iodide was replaced by benzyl bromide.
1H-NMR (CDC1 3 6: 1.46 9H), 2.84 (brs, 2H), 3.66 (brs, 2H), 4.23 (brs, 2H), 4.35 2H), 7.15 7.25 FDMS 374 (M 1).
Referential Example 4 5-t-butoxycarbonyl-4,5,6,7-tetrahydrothieno[2,3-c]pyridin- 2 carboxylic acid To the solution of 4,5,6,7-tetrahydrothieno[2,3c]pyridine hydrochloride (5.2 g) in DMF (60 ml) were added di-tbutyl dicarbonate (7.11 triethylamine (6.58 g) and a catalytic amount of 4-dimethylaminopyridine, and the mixture was stirred at room temperature for 1 hour. After the addition of ethyl acetate (300 ml) and washed with saturated saline, the mixture was dried over anhydrous magnesium sulfate. The solvent was removed by distillation. The residue was purified by column chromatography on silica gel to give 4.67 g of butoxycarbonyl-4,5,6,7-tetrahydrothieno[2,3-c]pyridine from the fraction eluted with n-hexane ethyl acetate 2 1 (yield, 88%).
IH-NMR (CDC1 3 6: 1.48 9H), 2.70 (brs, 2H), 3.68 (brs, 2H), 4.62 (brs, 2H), 6.79 J 5 Hz, 1H), 7.13 J 5 Hz, 1H).
FDMS 239 The solution of the compound prepared in (358 mg) in THF (5 ml) was cooled to -78"C, n-butyl lithium (2.5 M hexane solution, 0.96 ml) was added. The mixture was then stirred for 15 20 minutes. Carbon dioxide was blown into the reaction mixture for 30 minutes. Water was then added to the resulting mixture.
*After heating up to room temperature and the additiion of ethyl acetate (500 ml), the mixture was extracted with 5N sodium hydroxide. The aqueous layer was washed with ethyl acetate, and acidified to pH 4 with concentrated hydrochloric acid. After extraction with ethyl acetate, washing with water and drying over anhydrous magnesium sulfate, the solvent was removed by distillation to give 335 mg the title compound (yield, IH-NMR (CDCI 3 6: 1.49 9H), 2.72 (brs, 2H), 3.68 (brs, 2H), 25 4.66 (brs, 2H), 7.56 1H).
FDMS 283 Referential Example 5-t-butoxycarbonyl-5,6,7,8-tetrahydro-4H-thieno[3,2-c]azepin-2carboxylic acid The title compound was prepared in the same manner as in Referential Example 1.
EIMS 297 Referential Example 6 6-t-butoxycarbonyl-5,6,7,8-tetrahydro-4H-thieno[2,3-d]azepin-2carboxylic acid The title compound was prepared in the same manner as in Referential Example 1.
Referential Example 7 5-t-butoxycarbonyl-2-formyl-4,5,6,7-tetrahydrothieno[3,2c]pyridine To the solution of the compound prepared in Referential Example 1 (3.0 g) in THF (20 ml) was added a borane-methyl sulfide complex (1.1 ml), and the mixture was stirred at room temperature for 16 hours. After methanol was added to the reaction mixture, the solvent was removed by distillation. The residue was purified by column chromatography on silica gel to give 1.85 g of 5-t-butoxycarbonyl-2-hydroxymethyl- 4 ,5,6,7tetrahydrothieno[3,2-c]pyridine from the fraction eluted with 15 ethyl acetate n-hexane 1 3 (yield, 64.9%).
"H-NMR (CDC1 3 6: 1.48 9H), 1.82 J 6.1 Hz, 1H), 2.81 (brs, 2H), 3.71 (brs, 2H), 4.43 (brs, 2H), 4.75 J 6.1 Hz, 2H), 6.70 1H).
The solution of the compound prepared in (1.2 g) in 0* dichloromethane (20 ml) was added pyridinium chlorochromate (1.1 and the mixture was stirred at room temperature for 4 hours.
After the reaction mixture was filtered through FLORISIL, the filtrate was concentrated and purified by column chromatography on silica gel to give 1.07 g of the title compound from the 25 fraction eluted with ethyl acetate n-hexane 1 3 (yield, 89.8%).
1H-NMR (CDC1 3 6: 1.50 9H), 2.92 (brs, 2H), 3.74 (brs, 2H), 4.53 2H), 7.48 9.83 1H).
Referential Example 8 2-aminoacetyl-5-t-butoxycarbonyl-4,5,6,7-tetrahydrothieno-[3,2c]pyridine The solution of the compound prepared in Referential Example 7 (255 mg) in THF (10 ml) was cooled to -78"C, and methyl magnesium bromide (1.02 M THF solution, 1.0 ml) was added to the solution. The reaction was carried out at -78 C for 30 minutes and at -40'C for 10 minutes. After water was added, the mixture was extracted with ethyl acetate, washed with brine and dried over anhydrous magnesium sulfate. After the solvent was removed by distillation, the residue was purified by column chromatography on silica gel with an eluent system of ethyl acetate n-hexane 1 4 to give 203 mg of 2-(l-hydroxy)ethyl- 4 ,5,6,7-tetrahydrothieno[3,2-c]pyridine (yield, 1H-NMR (CDC1 3 6: 1.48 9H), 1.57 J 6.4 Hz, 3H), 1.98 (brs, 1H), 2.80 (brs, 2H), 3.71 (brs, 2H), 4.43 2H), 5.04 (q, J 6.4 Hz, 1H), 6.67 lH).
FDMS 283 (M To the solution of the compound prepared in (847 mg) in dichloromethane (30 ml) was added Molecular Sieves 4A (3 g), followed by pyridinium chlorochromate (966 mg) under ice-cooling.
15 The mixture was stirred for 40 minutes. After the reaction mixture was filtered through FLORISIL, the filtrate was concentrated to give 772 mg of 4,5,6,7-tetrahydrothieno[3,2-c]pyridine (yield, 92%).
IH-NMR (CDC13) 6: 1.49 9H), 2.51 3H), 2.88 (brs, 2H), 3.73 (brs, 2H), 4.50 2H), 7.39 (s,lH).
To the solution of the compound prepared in (342 mg) in dichloromethane (6 ml) was added triethylamine (0.42 ml), and the mixture was cooled to -35'C. After trimethylsilyl trifluoromethanesulfonate (0.31 ml) was further added, the 25 resulting mixture was stirred for 25 minutes. After Nbromosuccinimide (227 mg) was then added, the mixture was further stirred at -35 C for 10 minutes. The mixture was diluted with ether, washed with water, saturated aqueous sodium hydrogen carbonate and brine, and dried over anhydrous magnesium sulfate.
After the solvent was removed by distillation, the residue was dissolved in DMF (12 ml). Sodium azide (87 mg) was added to the solution, and the mixture was stirred at room temperature for 1 hour. The reaction mixture was diluted with ethyl acetate, washed with water and brine, and dried over anhydrous magnesium sulfate.
After the solvent was removed by distillation, the residue was purified by column chromatography on silica gel with an eluent system of ethyl acetate n-hexane 1 10 to give 188 mg of 2azidoacetyl-5-t-butoxycarbonyl-4,5,6,7-tetrahydrothieno[3,2c]pyridine (yield, 48%).
1H-NMR (CDC1 3 6: 1.49 9H), 2.90 (brs, 2H), 3.73 (brs, 2H), 4.39 2H), 4.51 2H), 7.39 1H).
FDMS 322 To the solution of the compound prepared in (188 mg) in ethanol (10 ml) was added IN hydrochloric acid (0.7 ml) and palladium-carbon (62 mg), and catalytic hydrogenation was performed at an ordinary temperature under atmospheric pressure for 70 minutes. After the catalyst was removed by filtration through celite, the filtrate was concentrated to give the title compound in the form of a hydrochlorlde.
1H-NMR 6:1.49 9H), 2.98 J 5.1 Hz, 2H), 3.76 (brs, 2H), 4.58 (brs, 2H), 4.60 2H), 7.75 1H).
Referential Example 9 Di-t-butyl [[4-(aminoacetyl)-o-phenylene]dioxy]diacetate Td the suspension of t-butyl bromoacetate (41.4 ml) and potassium carbonate (39 g) in acetone (500 ml) was added 4chloroacetylcatechol (25 and the mixture was stirred at room temperature for 16 hours. The reaction mixture was diluted with 4 ethyl acetate, washed with water and dried over anhydrous magnesium sulfate. After the solvent was removed by distillation, the residue was purified by column chromatography on silica gel 25 with an eluent system of ethyl acetate n-hexane 1 1 to give 29.1 g of a mixture of di-t-butyl [[4-(chloroacetyl)-ophenylene]dioxy]diacetate and di-t-butyl [[4-(bromoacetyl)-ophenylene]dioxy]diacetate.
To the solution of the mixture (15.25 g) in DMF (300 ml) was added sodium azide (2.4 and the mixture was stirred at room temperature for 3 hours. The reaction mixture was diluted with ethyl acetate, washed with water and dried over anhydrous magnesium sulfate. After the solvent was removed by distillation, the residue was purified by column chromatography on silica gel with an eluent system of ethyl acetate n-hexane 1 2 to give 11.2 g of di-t-butyl [[4-(azidoacetyl)-ophenylene]dioxy]diacetate (yield, 38%).
1H-NMR (CDC1 3 6: 1.48 9H), 1.49 9H), 4.49 2H), 4.66 2H), 4.68 2H), 6.82 J 9.2 Hz, 1H), 7.26 7.49 (m, 2H).
EIMS 421 To the solution of the compound prepared in (10 g) in methanol (200 ml) were added lN hydrochloric acid (30 ml) and palladium-carbon (500 mg), and catalytic hydrogenation was carried out at room temperature under atmospheric pressure for 4 hours. After the reaction mixture was filtered, the filtrate was concentrated, dissolved in water and lyophilized to give 10.2 g of the title compound in the form of a hydrochloride (yield, 99.6%).
1 H-NMR (DO) 6: 1.47 18H), 4.63 2H), 4.85 2H), 4.87 2H), 7.07 J 9.2 Hz, 2H), 7.53 7.72 2H).
Referential Example 3,4-(di-t-butoxycarbonylmethyloxy)benzoic acid To the solution of 3,4-dihydroxybenzoic acid (1.13 g) in benzene (50 ml) were added benzyl alcohol (3.8 ml) and ptoluenesulfonic acid (139 mg), and the mixture was heated at reflux in the presence of Molecular Sieves 4A for 2 days. Further amount of benzyl alcohol (1.5 ml) was added. The mixture was then heated at reflux overnight. After cooling by standing, saturated aqueous sodium hydrogen carbonate was added. The mixture was 25 extracted with ethyl acetate, washed with brine and dried over anhydrous magnesium sulfate. After the solvent was removed by distillation, the residue was dissolved in ethyl acetate and extracted with 1N sodium hydroxide. The aqueous layer was acidified with hydrochloric acid and extracted with ethyl acetate. After the organic layer was washed with brine, dried over anhydrous magnesium sulfate, the solvent was removed by distillation. The residue was purified by column chromatography on silica gel with an eluent system of chloroform methanol 1 to give 953 mg of benzyl 3,4-dihydroxybenzoate (yield, 53%).
1H-NMR (DMSO-D 6 6: 5.26 2H), 6.81 J 8.2 Hz, 1H), 7.36 7.60 7H), 9.38 1H), 9.81 1H).
EIMS 378 To the solution of the compound prepared in (860 mg) in DMF (12 ml) were added under ice-cooling potassium carbonate (1.02 g) and t-butyl bromoacetate (1.14 ml), and the mixture was stirred at room temperature for 2.5 hours. After dilution with ethyl acetate, the mixture was washed with water and saturated saline and dried over anhydrous magnesium sulfate. After the solvent was removed by distillation, the residue was purified by column chromatography on -silica gel with an eluent system of ethyl acetate n-hexane 1 10 to give 1.56 g of benzyl 3,4- (di-t-butoxycarbonylmethyloxy)benzoate (yield, 94%).
1H-NMR (CDC1 3 6: 1.45 9H), 1.47 9H), 4.63 2H), 4.65 2H), 6.80 J 8.4 Hz, 1H), 7.30 7.45 5H), 7.52 (d, 15 J 2.3 Hz, 1H), 7.71 (dd, J 2.3, 8.4 Hz, 1H).
S.EIMS 472 To the solution of the compound prepared in (562 mg) in ethanof (20 ml) was added 10% palladium-carbon (119 mg), and catalytic hydrogenation was carried out at room temperature under atmospheric pressure for 40 minutes. After the catalyst was removed by filtration with celite, the filtrate was concentrated to give 472 mg of the title compound (yield, 100%).
1H-NMR (CDC1 3 6: 1.48 9H), 1.49 9H), 4.65 2H), 4.68 2H), 6.83 J 8.6 Hz, 1H), 7.55 J 2.0 Hz, 1H), 7.74 25 (dd, J 2.0, 8.6 Hz, 1H).
EIMS 382 Referential Example 11 Di-t-butyl methylamino)acetyl]-o-phenylene] dioxy]diacetate To the solution of adrenalone hydrochloride (21.8 g) in DMF (200 ml) were added under ice-cooling benzyl chloroformate ml) and pyridine (22 ml), and the mixture was stirred at room temperature for 1 hour. After benzyl chloroformate (10 ml) and pyridine (11 ml) were further added to the mixture, reaction was further continued for 2 days. The reaction mixture was then 0 0seO @000 0O
S.
C.
@0 00 0 000 0
SS
0@
S
S
S.
concentrated under reduced pressure. The residue was dissolved in methanol (200 ml). After IN sodium hydroxide (300 ml) and sodium hydroxide (20 ml) were added to the solution, the mixture was stirred at room temperature for 1 hour. Methanol was removed by distillation, and the residue was washed with ether. After water was added to the ethereal solution, the mixture was acidified with 5N hydrochloric acid under ice-cooling to pH The precipitates were collected by filtration, washed with water and ether and dried to give 17.9 g of N-benzyloxy carbonyladrenalone (yield, 56.8%).
1 H-NMR (DMSO-D 6 6: 3.90 and 3.96 (double sets of s, 3H), 4.65 and 4.69 (double sets of s, 2H), 5.02 and 5.10 (double sets of 6.81 (brs, 1H), 7.16 7.45 7H).
EIMS 315 15 To the solution of the compound prepared in (1.23 g) in DMF (15 ml) were added t-butyl bromoacetate (1.3 ml) and potassium carbonate (2.2 and the mixture was stirred at 60 C for 3 hours. The reaction mixture was diluted with ethyl acetate, washed with water and dried over anhydrous magnesium sulfate.
After the solvents were removed by distillation, the residue was purified by column chromatography on silica gel with an eluent system of chloroform ethyl acetate 7 1 to give 2.08 g of di-t-butyl [[4-[(N-benzyloxycarbonyl-N-methylamino)acetyl]-ophenylene]dioxy]diacetate (yield, 98%).
IH-NMR (CDC1 3 6: 1.47 9H), 1.48 9H), 3.00 and 3.01 (double sets of s, 3H), 4.64 2H), 4.68 2H), 5.10 and 5.18 (double sets of s, 2H), 6.78 and 6.81 (double sets of d, J 8.6 Hz, 1H), 7.23 7.41 5H), 7.44 and 7.48 (double sets of d, J 1.9 Hz, 1H), 7.49 and 7.57 (double sets of dd, J 1.9, 8.6 Hz, 1H).
To the solution of the compound prepared in (4 g) in methanol (50 ml) were added IN hydrochloric acid (7.5 ml) and palladium-carbon (400 mg), and catalytic hydrogenation was carried out at an ordinary temperature under atmospheric pressure for 3 hours. After the reaction mixture was filtered, the filtrate was concentrated. Water was then added to the residue.
The mixture was lyophilized to give 3.3 g of the title compound 0~ in the form of a hydrochloride (yield, 100%).
1 H-NMR (CD 3 0D) 6: 1.49 (18H), 2.80 3H), 4.65 2H), 4.75 2H), 4.86 2H), 7.04 J 8.5 Hz, 1H), 7.58 J 2.1 Hz, 1H), 7.69 (dd, J 2.1, 8.5 Hz, 1H).
Referential Example 12 Diphenylmethyl 3-(4-aminoacetyl)phenylpropionate To the solution of 3-(4-chloroacetyl) phenylpropionate (3 g) in methanol was added diphenyldiazomethane (2.57 and the mixture was stirred at room temperature for 16 hours. After the reaction solution was filtered, the filtrate was concentrated and purified by column chromatography on silica gel with an eluent system of ethyl acetate n-hexane 1 2 to give 4 g of diphenylmethyl 3-(4-chloroacetyl)phenylpropionate (yield, 76.9%).
15 1H-NMR (CDC1 3 6: 2.77 J 7.7 Hz, 2H), 3.04 J 7.7 Hz, 2H), 4.65 2H), 6.87 1H), 7.24 7.35 12H), 7.81 (d, J 8.2 Hz, 2H).
To the solution of the compound prepared in (3.55 g) in DMF (10 ml) was added sodium azide (1.17 and the mixture 20 was stirred at room temperature for 3 hours. The reaction mixture was diluted with ethyl acetate, washed with water and dried over anhydrous magnesium sulfate. After the solvents were removed by distillation, the residue was purified by column chromatography on silica gel with an eluent system of ethyl acetate n-hexane 1 3 to give 3.3 g of diphenylmethyl 3-(4azidoacetyl)phenylpropionate (yield, 83.9%).
1H-NMR (CDC1 3 6: 2.28 J 7.4 Hz, 2H), 3.05 J 7.4 Hz, 2H), 4.51 2H), 6.87 1H), 7.25 7.31 12H), 7.77 (d, J 8.5 Hz, 2H).
To the solution of the compound prepared in (1 g) in methanol (20 ml) were added IN hydrochloric acid and palladium-carbon (50 mg), and catalytic hydrogenation was carried out at room temperature under atmospheric pressure for 3 hours.
After the reaction mixture was filtered, the filtrate was concentrated, dissolved in water and then lyophilized to give 1 g of the title compound in the form of a hydrochloride (yield, 97.4%).
1H-NMR (CD 3 0D) 6: 2.84 J 7.4 Hz, 2H), 3.06 J 7.4 Hz, 2H), 4.53 2H), 6.80 1H), 7.24 7.31 10H), 7.38 (d, J 8.5 Hz, 2H), 7.88 J 8.5 Hz, 2H).
Referential Example 13 2-(2-amino-l-hydroxy)ethyl-5- (t-butoxycarbonylmethyl)oxypyridine The solution of 5-hydroxy-2-hydroxymethylpyridine (5.0 g) in DMF (10 ml) was titrated under ice-cooling in the suspension of sodium hydride (60% purity, 1.6 g) in DMF (10 ml). After minutes, the mixture was heated to room temperature and stirred for 15 minutes. After t-butyl bromoacetate (6 ml) was added to the mixture under ice-cooling, the resulting mixture was stirred under ice-cooling for 10 minutes and at room temperature for minutes. The reaction mixture was diluted with ethyl acetate, washed with water and brine and dried over anhydrous magnesium sulfate. After the solvents were removed by distillation, the *residue was purified by column chromatography on silica gel with an eluent system of chloroform chloroform methanol 50 1 to give 8.16 g of 5-(t-butoxycarbonylmethyl)oxy- 2 hydroxymethylpyridine (yield, H"-NMR (CDC1 3 6: 1.50 9H), 3.35 3.45 (brs, 1H), 4.56 (s, 2H), 4.70 2H), 7.19 J 8.5 Hz, 1H), 7.23 (dd, J 2.8, Hz, 1H), 8.25 J 2.8 Hz, 1H).
EIMS 239 25 To the solution of the compound prepared in (10.5 g) in dichloromethane (250 ml) was added manganese dioxide (19 g) under ice-cooling. The mixture was stirred at room temperature for 5 hours, during which 10 g of manganese dioxide was added every 1 to 1.5 hours. After the reaction mixture was filtered through celite, the filtrate was concentrated to give 8.95 g of 5-(t-butoxycarbonylmethyl)oxy-2-formylpyridine (yield, 86%).
1H-NMR (CDC1 3 6: 1.50 9H), 4.66 2H), 4.70 2H), 7.28 (dd, J 2.7, 8.6 Hz, 1H), 7.97 J 8.6 Hz, 1H), 8.45 J 2.7 Hz, 1H), 10.0 1H).
EIMS 237 The solution of the compound prepared in (6.2 g) in THF (200 ml) was cooled to -40:C, and methyl magnesium bromide (1.02 M THF solution, 27 ml) was titrated. After 30 minutes, water was added to the mixture. The mixture was extracted with ethyl acetate, washed with brine and dried over anhydrous magnesium sulfate. After the solvents were removed by distillation, the residue was purified by column chromatography on silica gel with an eluent system of ethyl acetate n-hexane 1 1 to give 5.2 g of 5-(t-butoxycarbonylmethyl)oxy-2-(lhydroxy)ethylpyridine (yield, 79%).
1H-NMR (CDC1 3 6: 1.48 J 6.5 Hz, 3H), 1.50 9H), 3.91 J 4.6 Hz, 1H), 4.56 2H), 4.86 (dq, J 4.6, 6.5 Hz, 1H), 7.22 7.23 2H), 8.22 1.5 Hz, 1H).
EIMS 253 The compound prepared in (3.31 g) was treated in the 15 same manner as in to give 3.33 g of butoxycarbonylmethyl)oxypiridine (yield, 100%).
1H-NMR (CDC13) 6: 1.50 9H), 2.68 3H), 4.63 2H), 7.23 (dd, J 3.1, 8.6 Hz, 1H), 8.04 J 8.6 Hz, 1H), 8.34 J S= 3.1 Hz, 1H).
20 EIMS 251 To the solution of the compound prepared in (468 mg) in dichloroethane (10 ml) was added triethylamine (0.65 ml), and trimethylsilyl trifluoromethanesulfonate (0.4 ml) was titrated under ice-cooling. The mixture was stirred for 30 minutes. The 25 solvent was then evaporated under reduced pressure. The residue was extracted with ether. After ether was evaporated, the residue was dissolved in THF (10 ml), N-bromosuccinimide (353 mg) was added under ice-cooling. After the mixture was stirred for minutes, the mixture was diluted with ether, washed with water, saturated sodium hydrogen carbonate and brine, and dried over anhydrous magnesium sulfate. After the solvents were removed by distillation, hexane was added to the residue thus obtained.
Crystals were collected by filtration to give 451 mg of 2- (yield, 73%).
1H-NMR (CDC1 3 6: 1.50 9H), 4.56 2H), 4.64 2H), 4.81 2H), 7.25 (dd, J 2.8, 8.6 Hz, 1H), 8.10 J 8.6 Hz, 1H), 8.34 J 2.8 Hz, 1H).
EIMS 329, 331 To the solution of the compound prepared in (427 mg) in DMF (10 ml) was added sodium azide (95 mg), and the mixture was stirred for 1 hour. The reaction mixture was diluted with ethyl acetate, washed with water and brine and dried over anhydrous magnesium sulfate. After the solvents was removed by distillatio, the residue was purified by column chromatography on silica gel with an eluent system of toluene ethyl acetate 30 1 to give 297 mg of butoxycarbonylmethyl)oxypyridine (yield, 79%).
1 H-NMR (CDC1 3 6: 1.50 9H), 4.64 fs, 2H), 4.81 2H), 7.26 (dd, J 2.8, 8.6 Hz, 1H}, 8.08 J 8.6 Hz, 1H), 8.31 J 2.8 Hz, 1H).
SIMS 293 (M 1).
To the solution of the compound prepared in in ethanol (4 ml) were added IN hydrochloric acid (0.52 ml) and palladium-carbon (22 mg), and catalytic hydrogenation was carried out at room temperature under atmospheric pressure for 1 hour.
After the reaction mixture was filtered through celite, the filtrate was concentrated to give 72 mg of the title compound in the form of a hydrochloride (yield, 100%).
H-NMR (D20) 6: 1.52 9H), 3.31 (dd, J 9.0, 13.1 Hz, 1H), 3.55 (dd, J 3.3, 13.1 Hz, 1H), 4.94 2H), 5.40 (dd, J 3.3, 9.0 Hz, 1H), 7.97 J 9.0 Hz, 1H), 8.11 (dd, J 2.8, 9.0 Hz, 1H), 8.50 J 2.8 Hz, 1H).
SIMS 269 (M 1).
Referential Example 14 Ethyl 4-(2-aminoethyl)phenoxyacetate To the solution of tyramine (5 g) in DMF (50 ml) were added triethylamine (5 ml) and di-t-butyl dicarbonate (8.4 ml), and the mixture was stirred at a temperature of ice-cooling to room temperature. After the reaction mixture was concentrated under reduced pressure, the residue was diluted with ethyl acetate, washed with water and dried over anhydrous magnesium 2 hours. After the reaction mixture was concentrated under reduced pressure, the residue was diluted with ethyl acetate, washed with water and dried over anhydrous magnesium sulfate.
After the solvents were removed by distillation, crystals thus obtained were washed with n-hexane to give 17.7 g of t-butyl 4acetylphenoxyacetate (yield, 96%).
1 H-NMR (CDC1 3 6: 1.49 9H), 2.56 3H), 4.58 2H), 6.93 J 9.2 Hz, 2H), 7.94 J 9.2 Hz, 2H).
EIMS 250 To the solution of the compound prepared in (500 mg) in 1,2-dichloroethane (5 ml) were added triethylamine (0.68 ml) followed by trimethylsilyl trifluoromethanesulfonate (0.44 ml), and the mixture was stirred at room temperature for 1 hour. After trimethylsilyl trifluoromethanesulfonate (0.1 ml) was added, the mixture was further stirred for 30 minutes. The reaction mixture was concentrated, and the residue was extracted with ether by Sdecantation. After the ether was evaporated, the residue thus obtained was dissolved in THF (5 ml), N-bromosuccinimide (374 mg) was added under ice-cooling. The mixture was then stirred at room 20 temperature for 1.5 hours. The reaction mixture was diluted with ether, washed with water, saturated aqueous sodium hydrogen carbonate and water in this sequence, and dried over anhydrous magnesium sulfate. The solvents were evaporated to give 650 mg of t-butyl 4- bromoacetylphenoxyacetate (yield, 25 1 _NMR (CDC1 3 6: 1.49 9H), 4.40 2H), 4.60 2H), 6.95 J 9.0 Hz, 2H), 7.97 J 9.0 Hz, 2H).
EIMS 330 To the solution of the compound prepared in (660 mg) in DMF (6 ml) was added sodium azide (156 mg), and the mixture was stirred at room temperature for 1 hour. The reaction mixture was diluted with ethyl acetate, washed with water and dried over anhydrous magnesium sulfate. After the solvents were evaporated, the residue was purified by column chromatography on silica gel with an eluent system of n-hexane ethyl acetate 5 1 to give 537 mg of t-butyl 4-azidoacetylphenoxyacetate (yield, 92%).
1H-NMR (CDC1 3 6: 1.49 9H), 4.51 2H), 4.59 2H), 6.95 sulfate. The solvents were then removed by distillation. The residue was purified by column chromatography on silica gel with an eluent system of chloroform emthanol 30 1 to give g of N-t-butoxycarbonyltyramine (yield, 99%).
1 H-NMR (CDC1 3 6: 1.44 9H), 2.70 (brs, 2H), 3.32 (brs, 2H), 6.79 J 8.5 Hz, 2H), 7.00 J 8.5 Hz, 2H).
FDMS 237 (M To the solution of the compound prepared in (2 g) in DMF (20 ml) were added ethyl bromoacetate (0.98 ml) and potassium carbonate (1.4 and- the mixture was stirred at room temperature overnight. The reaction mixture was diluted with ethyl acetate, washed with water -and dried over anhydrous magnesium sulfate. After the solvents were evaporated, the residue was purified by column chromatography on silica gel with 15 an eluent system of chloroform emthanol =50 1 to give 2.5 g of ethyl [4-(2-t-butoxycarbonylamino) ethyl]phenoxyacetate (yield, 1H-NMR (CDCl 3 6: 1.30 J 6.9 Hz, 3H), 1.43 9H), 2.73 J 6.9 Hz, 2H), 3.33 J 6.9 Hz, 2H), 4.28 J 6.9 20 Hz, 2H), 6.85 J 8.6 Hz, 2H), 7.11 J 8.6 Hz, 2H).
EIMS 323 To the solution of the compound prepared in (2 g) in anisole (3.3 ml) was added trifluoroacetic acid (3 ml), and the mixture was stirred at room temperature for 3 hours. The reaction 25 mixture was concentrated under reduced pressure. The residue was washed with n-hexane, dissolved in water and lyophilized to give the title compound in the form of a trifluoroacetate.
1 H-NMR (CDC1 3 6: 1.30 J 7.2 Hz, 3H), 2.69 J 6.9 Hz, 2H), 2.93 J 6.9 Hz, 2H), 4.28 J 7.2 Hz, 2H), 4.60 (s, 2H), 6.85 J 8.6 Hz, 2H), 7.11 J 8.6 Hz, 2H).
Referential Example t-butyl 4- (aminoacetyl)phenoxyacetate To the solution of p-acetylphenol (10 g) in DMF (50 ml) were added potassium carbonate (12.2 g) and t-butyl bromoacetate (16.2 ml), and the mixture was stirred at room temperature for J 8.9 Hz, 2H), 7.89 J 8.9 Hz, 2H).
To the solution of the compound prepared in (537 mg) in ethanol (8 ml) were added IN hydrochloric acid (2.2 ml) and palladium-carbon (50 mg), and catalytic hydrogenation was performed at room temperature under atmospheric pressure for hours. The reaction mixture was filtered through celite. After the filtrate was concentrated, the residue was dissolved in water, washed with ether and lyophilized to give the title compound in the form of a hydrochloride.
1H-NMR (CD 3 OD) 6: 1.48 9H), 4.52 2H), 4.72 2H), 7.05 J 8.9 Hz, 2H), 8.01 J 8.9 Hz, 2H).
FDMS 265 Referential Example 16 Di-t-butyl The title compound was prepared from Sdihydroxyacetophenone according to the same manner as in Referential Example 1 H-NMR (CD30D) 6: 1.51 18H), 4.56 2H), 4.59 2H), 4.68 20 2H), 6.62 1H), 6.84 2H).
EIMS 395 *Referential Example 17 t-butyl 3-(aminoacetyl)phenoxyacetate 25 The title compound was prepared from m-acetylphenol according to the same manner as in Referential Example 1H-NMR (CD3OD) 6: 1.49 9H), 4.57 2H), 4.68 2H), 7.27 1H), 7.48 7.54 2H), 7.65 1H).
Referential Example 17 t-Butyl 3-(aminoacetyl)phenoxyacetate The title compound was prepared from m-acetylphenol according to the same manner as in Referential Example 1H-NMR (CD 3 0D) 6: 1.49 9H), 4.57 2H), 4.68 2H), 7.27 1H), 7.48 7.54 2H), 7.65 1H).
Referential Example 18 Di-t-butyl [[4-(aminoacetyl)-m-phenylene]dioxy]diacetate The title compound was prepared from dihydroxyacetophenone according to the same manner as in Referential Example 1H-NMR (CD 3 0D) 6: 1.57 9H), 1.59 9H), 4.63 2H), 4.78 2H), 4.88 2H), 6.69 1H), 6.75 J 8.8 Hz, 1H), 8.08 J 8.8 Hz, 1H).
Referential Example 19 t-Butyl [4-(aminoacetyl)-2-methoxy]phenoxyacetate To the solution of 4-chloroacetylcatechol (25 g) in DMF (200 ml) was added sodium azide (10.5 and the mixture was stirred at room temperature for 1 hour. The reaction mixture was diluted with ethyl acetate, washed with water and dried over anhydrous magnesium sulfate. After the solvent was evaporated, the solids thus obtained was washed with a solution of n-hexane ether 1 to give 23.2 g of 4-azidoacetylcatechol (yield, 20 1 H-NMR (CD30D) 6: 4.59 2H), 6.84 J 8.3 Hz, 1H), 7.39 2H).
EIMS 193 To the solution of the compound prepared in (1 g) in acetone (10 ml) were added t-butyl bromoacetate (0.76 ml) and 25 potassium carbonate (716 mg), and the mixture was stirred at room S. temperature for 1.5 hours. The reaction mixture was diluted with ethyl acetate, washed with water and dried over anhydrous magnesium sulfate. After the solvents were evaporated, the residue was purified by column chromatography on silica gel with an eluent system of chloroform methanol 50 1 to give 318 mg of t-butyl 4-azidoacetyl-3-hydroxyphenoxyacetate (yield, 1H-NMR (CDC1 3 6: 1.50 9H), 4.49 2H), 4.61 2H), 6.89 J 8.2 Hz, 1H), 7.45 (dd, J 2.1, 8.2 Hz, 1H), 7.49 J 2.1 Hz, 1H).
To the solution of the compound prepared in (500 mg) in acetone (5 ml) was added potassium carbonate (340 mg) and methyl iodide (0.5 ml), and the mixture was stirred at room temperature for 1 hour. After additional amount of methyl iodide ml) was added, the mixture was further stirred at 50:C for 1 hour. The reaction mixture was diluted with ethyl acetate, washed with water and dried over anhydrous magnesium sulfate.
After the solvents were eveporated, the residue was purified by column chromatography on silica gel with an eluent system of chloroform ethyl acetate 50 1 to give 400 mg of t-butyl 4azidoacetyl-3-methoxyphenoxyacetate (yield, 76%).
1H-NMR (CDC1 3 6: 1.47 9H), 3.95 3H), 4.51 2H), 4.67 2H), 6.77 J 8.5 Hz, lH), 7.42 (1H, dd, J 2.0, Hz), 7.54(1H, d, EIMS 321 The title compound in the form of a hydrochloride was obtained according to the method described in Referential Example 15 -NMR (CD 3 OD) 6: 1.48 9H), 3.93 3H), 4.54 2H), 4.74 2H), 6.97 J 8.6 Hz, 1H), 7.60 J 1.0 Hz, 1H), 7.64 (dd, J 1.9, 8.6 Hz, 1H).
EIMS 295 Referential Example Diethyl [4-(2-aminoethyl)-o-phenylene]dioxy]diacetate The title compound was prepared as the trifluoroacetate 25 according to the same manner as in Referential Example 14.
1H-NMR (CDC13) 6: 1.29 6H), 2.90 2H), 3.21 2H), 4.25 4H), 4.63 2H), 4.64 2H), 6.79 3H), 7.95 (brs, 2H).
Referential Example 21 Diethyl [[4-(aminoacetyl)-o-phenylene]dioxy]diacetate The solution of the compound prepared in Referential Example 19 (13 g) in acetone (100 ml) was added to the solution of ethyl bromoacetate (15.7 ml) and potassium carbonate (19.5 g) in acetone (100 ml), and the mixture was stirred at room temperature for 12 hours. After insolubles were removed by filtration, the solvents were evaporated. Crystals obtained were collected by filtration, washed with ether and dried to give 11.1 g of diethyl [[4-(azidoacetyl)-o-phenylene]dioxy]diacetate.
IH-NMR (CDC1 3 6: 1.29 J 7 Hz, 3H), 1.31 J 7 Hz, 3H), 4.27 J 7 Hz, 2H), 4.28 J 7 Hz, 2H), 4.49 2H), 4.77 2H), 4.80 2H), 6.86 J 8 Hz, 1H), 7.49 J 8Hz, 1H), 7.51 1H).
To the solution of the compound prepared in (1 g) in ethanol (45 ml) were added IN hydrochloric acid (4.9 ml) and palladium-carbon (90 mg), and catalytic hydrogenation was performed at room temperature under atmospheric pressure for 1 hour. After the catalyst was removed by filtration through celite, the solvent was evaporated. Crystals deposited were collected by filtration, washed with ether and dried to give the 15 title compound as the hydrochloride.
1H-NMR (CDC13 CD3OD) 6: 1.31 J 7 Hz, 6H), 4.27 J 7 SHz, 2H), 4.28 J 7 Hz, 2H), 4.47 (brs, 2H), 4.79 2H), 4.82 2H), 6.90 J 8 Hz, 1H), 7.55 1H), 7.63 J 8Hz, 1H).
Example 18 6, 7-tetrahydrothieno[3,2-c]pyridin- 2 yl]carbonyl]amino]acetyl]-o-phenylene]dioxy]diacetic acid trifluoroacetate 25 To the solution of the compound prepared in Referential Example 1 (3.0 g) in DMF (20 ml) were. added benzotriazole-1yloxytris(dimethylamino)phosphonium hexafluorophosphate (7.0 g) and N-methylmorpholine (2.3 ml), and the mixture was stirred at room temperature for 1 hour. After the compound prepared in Referential Example 9 (4.6 g) was added, the mixture was stirred at room temperature for 1 hour. The solvent was evaporated under reduced pressure. The residue was then taken into ethyl acetate.
The mixture was washed with water and dried over anhydrous magnesium sulfate. After the solvent was evaporated, the residue was purified by column chromatography on silica gel to give 3.56 g of di-t-butyl [[4-[[[(5-t-butyloxycarbonyl- 4 ,5, 6 7 tetrahydrothieno[3,2-c]pyridin-2-yl)carbonyl]amino]acetyl]-ophenylene ]dioxy ]di acetate from the fraction eluted with ethyl acetate n-hexane 1 1 (yield, 50.9%).
'H-NMR (CDCl1 3 6: 1.48 -1.58(27H), 2.87 (brs, 2H), 3.73 (brs, 2H), 4.49 2H), 4.67 2H), 4.70 2H), 4.84 J 4.1 Hz, 1H), 6.85 8.7Hz, 1H), 7.08 (brs, 1H), 7.33 1H), 7.50 J 1.9 Hz, 1H), 7.63 (dd, J 1.9, 8.3 Hz, 1H).
To the aforementioned compound (3.56 g) were added anisole (5 ml) and trifluoroacetic acid (20 ml), and the mixture was stirred at room temperature for 3 hours. After isopropyl ether was added to the reaction mixture, crystals deposited were collected by filtration to give 2.95 g of the title compound IH-NMR (DMSO-d 6 6: 3.08 (in, 2H), 3.37 3.61 (in, 2H), 4.24 (s, 2H), 4.70 2H), 4.79 2H), 4.85 2H), 7.02 J 8.8 Hz, 1H), 7.42 J 1.8 Hz, 1H), 7.60 1H), 7.70 (dd J 1.8, 8.8 Hz, 1H), 8.82 (brs, 1H), 9.09 (brs, 1H).
The compounds of Example 19 -38 were prepared in the same manner as in Example 18.
Example 19 4 4 5 ,6,7tetrahydrothieno[3,2c] pyridin2yl)carbonyl]
N-
trifluoroacetate The title compound was prepared from the compounds of 25 Referential Examples 1 and 11.
Di-t-butyl [[4-[[[(5-t-butyloxycarboflyl- 4 ,5, 6 7 terhdoheo32cpyii--lcroy]N methylaminol acetyl] -o-phenylene] dioxy] diacetate 'H-NMR (CDCl 3 6: 1.48 (27H), 2.83 (brs, 2H), 3.31 (brs, 3H), 3.71 (brs, 2H), 4.46 (brs, 2H), 4.68 2H), 4.82 (brs, 2H), 4.90 2H), 6.82 7.50 (in, 4H).
Title compound 'H-NMR (DMSO-d 6 6: 3.07 (brs, 2H), 3.33 (brs, 3H), 3.45 (brs, 2H), 4.22 (brs, 2H), 4.78 2H), 4.85 2H), 4.95 (brs, 2H), 7.01 7.66 (mn, 4W).
Example 6,7-tetrahydrothieno[3,2-clpyridin-2yl)carbonyl]aminoacetyl]Phenyl]propionic acid trifluoroacetate The title compound was prepared from the compounds of Referential Examples 1 and 12.
Diphenylmethyl 3-[4-[[(5-t-butyloxycarbonyl-4,5, 6 7 tetrahydrothieno[3,2-c]pyridin-2-yl)carbonyllaminoacetylI phenyl]propionate IH-NMR (CDC1 3 6: 1.50 9H), 2.79 J 7.5 Hz, 2H), 2.88 2H), 3.06 J 7.5 Hz, 2H), 3.74 (brs, 2H), 4.51 (brs, 2H), 4.87 J 4.2 Hz, 2H), 6.87 1H), 7.03 (brs, 1H), 7.28 7.34 10H), 7.87 J 8.3 Hz, 1H).
FDMS 638 Title compound 1 H-NMR (CD 3 OD) 5: 2.65 J 7.4 Hz, 2H), 3.00 J 7.4 Hz, 33 2H), 3.20 J 6.2 Hz, 2H), 3.57 J 6.2 Hz, 2H), 4.32 (s, 2H), 4.87 2H), 7.41 J 8.5 Hz, 2H), 7.97 J 8.5 Hz, 2H).
FDMS 372 Example 21 2 -l hydroxy-2-[(4,5,6,7-tetrahydrothieno[3,2-c]pyridin-2- 2-[-5-yloxyacetic acid trifluoroacetate The title compound was prepared from the compounds of iReferential Examples 1 and 13.
t-Butyl 2-[2-[(5-t-butoxycarbonyl-4,S, 6 7 tetrahydrothieno[3,2-cpyridin-2-yl)-carbonylaminoll 'H-NMR (CDCl 3 6: 1.48 9H), 1.49 9H), 2.84 (brs, 2H), 3.57 1H), 3.60 (ddd, J 2.8, 6.4, 13.8 Hz, 1H), 3.71 (brs, 2H), 4.46 2H), 4.56 2H), 4.60 4.80 (brs, 1H), 4.90 (dd, J 2.8, 6.4 Hz, 1H), 6.51 (brt, 1H), 7.18 1H), 7.23 (dd, J 2.8, 8.3 Hz, 1H), 7.37 J 8.6 Hz, 1H), 8.24 J 2.8 Hz, 1H).
EIMS 533 Title compound 1H-NMR (D0O) 6: 3.20 J 6.2 Hz, 2H), 3.59 J 6.2 Hz, 2H), 3.82 (ABbq, J 5.1, 1.14, 4 Hz, 1H), 4.32 2H), 4.97 (s, 2H), 5.34 J 5.1 Hz, 1H), 7.42 1H), 8.01 J 9.0 Hz, 1H), 8.16 (dd, J 2.8, 9.0 Hz, 1H), 8.44 J 2.8 Hz, 1H).
SIMS 378 (M 1).
Example 22 5,6, 7-tetrahydrothieno [3 ,2-c]pyridin-2yl)carbonyl]aminoacetyl]phenoxyacetic acid trifluoroacetate The title compound was prepared from the compounds of Referential Examples 1 and t-butyl 4-[[(5-t-butoxycarbonyl- 4 ,5, 6 7 tetrahydrothieno[3,2-c]pyridin-2-yl)carbonyl] 15 aminoacetyl]phenoxyacetate IH-NMR (CDC1 3 6: 1.49 9H), 2.87 (brs, 2H), 3.73 (brs, 2H), 4.50 2H), 4.61 2H), 4.85 J 3.9 Hz, 2H), 6.98 (d, J 8.2 Hi, 2H), 7.05 (brs, 1H), 7.33 1H), 8.00 J 8.2 .Hz, 2H).
20 EIMS 530 Title compound 1 H-NMR (CD30D) 6: 3.21 J 6.2 Hz, 2H), 3.57 J 6.2 Hz, 2H), 4.32 2H), 4.78 2H), 4.81 2H), 7.06 J Hz, 2H), 7.5 1H), 8.03 J 9.0 Hz, 1H).
25 FDMS 375 (M 1).
Example 23 [[5-[[[(4,5,6,7-tetrahydrothieno[ 3 ,2-c]pyridin-2yl)carbonyl]amino]acetyl]-m-phenylene]dioxy]diacetic acid trifluoroacetate The title compound was prepared from the compounds of Referential Examples 1 and 16.
Di-t-butyl [[5-[[[(5-t-butoxycarbonyl-4,5,6,7tetrahydrothieno[3,2-c]pyridin-2-yl)carbonyl]amino]acetyl]-mphenylene]dioxy]diacetate IH-NMR (CDC1 3 6: 1.50 9H), 1.51 (18H), 2.87 (brs, 2H), 3.74 (brs, 2H), 4.50 (brs, 2H), 4.55 4H), 4.84 J 4.4 Hz, 2H), 6.76 J 2.2 Hz, 1H), 6.94 (brs, 1H), 7.14 J 2.2 Hz, 1H), 7.33 1H).
Title compound IH-NMR (CD30D) 6: 3.20 J 6.4 Hz, 2H), 3.57 J 6.4 Hz, 2H), 4.32 2H), 4.74 4H), 4.80 2H), 6.84 J 2.2 Hz, 1H), 7.2 J 2.2 Hz, 2H), 7.54 1H).
FDMS 449 (M 1).
Example 24 3 5,6 7-tetrahydrothieno[3.2-c]pyridin- 2 yl)carbonyl]aminoacetyl]phenoxyacetic acid trifluoroacetate The title compound was prepared from the compounds of Referential Examples 1 and 17.
t-Butyl 3- (5-t-butoxycarbonyl-4,5,6,7tetrahydrothieno[3,2-c]pyridin-2-yl)carbonyl] a. aminoacetyl]phenoxyacetate 1 H-NMR (CDCl 3 6: 1.50 9H), 1.51 9H), 2.88 2H), 3.74 2H), 4.50 2H), 4.59 2H), 4.89 J 4.4 Hz, 2H), 20 6.99 (brs, 1H), 7.20 (dd, J 2.6, 8.1 Hz, 1H), 7.44 J 8.1 Hz, 1H), 7.50 1H), 7.63 J 7.5 Hz, 1H).
Title compound -1H:-NMR (CD 3 0D) 6: 3.22 (brs, 2H), 3.58 (brs, 2H), 4.32 (brs, 2H), 4.75 2H), 4.84 2H), 7.25 J 8.0 Hz, 1H), 7.48 (t, 25 J 8.0 Hz, 1H), 7.56 2H), 7.68 J 7.4 Hz, 1H).
FDMS 375 (M 1).
Example 4,5, 6, 7-tetrahydrothieno 2-c] pyridin-2yl)carbonyl]amino]acetyl]-m-phenylene]dioxy]diacetic acid trifluoroacetate The title compound was prepared from the compounds of Referential Examples 1 and 18.
Di-t-butyl [[4-[[[(5-t-butoxycarbonyl-4,5, 6 7 tetrahydrothieno[ 3 ,2-c]pyridin-2-yl)carbonyl]amino]acetyl] -mphenylene]dioxy]diacetate 1 H-NMR (CDCl 3 6: 1.49 9H), 1.50 9H), 2.85 (brs, 2H), 3.72 (brs, 2H), 4.48 (brs, 2H), 4.55 2H), 4.67 2H), 4.87 J 4.6 Hz, 2H), 6.40 J 2.1 Hz, 1H), 6.52 (dd, J 2.1, 8.9 Hz, 1H), 7.30 1H), 7.99 J 8.9 Hz, 1H).
Title compound 1 H-NMR (CD3OD) 6: 3.19 (brs, 2H), 3.57 (brs, 2H), 4.31 (brs, 2H), 4.76 2H), 4.83 2H), 6.64 2H), 7.52 1H), 7.88 (d, J 8.6 Hz, 1H).
FDMS 449 (M 1).
i« **15
S
i a
C
Example 26 5, 6, 7-tetrahydrothieno[3. 2-c]pyridin-2yl)carbonyl]aminoacetyl]-2-methoxyphenoxyacetic acid trifluoroacetate The title compound was prepared from the compounds of Referential Examples 1 and 19.
t-Butyl [[(5-t-butoxycarbonyl-4,5,6,7-tetrahydrothieno [3,2-c]pyfidin-2-yl)carbonyl]aminoacetyl]-3-methoxyphenoxyacetate IH-NMR (CDCl 3 6: 1.48 9H), 1.50 9H), 2.89 (brs, 2H), 3.74 (brs, 2H), 3.97 3H), 4.50 (brs, 2H), 4.68 2H), 4.86 J 4.2 Hz, 2H), 6.81 J 8.3 Hz, 1H), 7.04 (brs, 1H), 7.34 1H), 7.56 J 2.0 Hz, 1H), 7.61 (dd, J 2.0, 8.3 Hz, 1H).
Title compound 1H-NMR (CD 3 0D) 6: 3.20 J 5.9 Hz, 2H), 3.57 J 5.9 Hz, 2H), 3.92 3H), 4.31 (brs, 2H), 4.80 2H), 4.82 2H), 6.99 J 8.5 Hz, 1H), 7.54 1H), 7.60 J 1.8 Hz, 1H), 7.68 (dd, J 1.8, 8.5 Hz, 1H).
FDMS 405 (M 1).
s-p 2 C *rr Example 27 4 -[[[(3-methyl-4,5,6,7-tetrahydrothieno[3.2-c]pyridin- 2 yl)carbonyl]amino]acetyl]-o-phenylene]dioxy]diacetic acid trifluoroacetate The title compound was prepared from the compounds of Referential Examples 2 and 9.
Di-t-butyl [[4-[[[(5-t.-butoxycarbofl-3-methyl-4,5,6,7tetrahydrothieflo[3,2-c]pyridin- 2 -yl )carbonyllamiflacetyl]-ophenylenel dioxy] diacetate 'H-NMR (CDCl 3 6: 1.48 9H), 1.50 9H), 2.42 3H), 2.84 (brs, 2H), 3.72 (brs, 2H), 4.38 (brs, 2H), 4.66 3H), 4.70 (s, 2H), 4.84 J= 4.2 Hz, 2H), 6.85 J 8.3 Hz, 1H), 6.90 (brs, 1H), 7.51 J 2.0 Hz, 1H), 7.63 (dd, J 2.0, 8.3 Hz, 1H).
SIMS 675 1).
Title compound IH-NMR (DMSO-d 6 6: 2.32 3H), 3.04 (brs, 2H), 3.43 (brs, 2H), 4.14 2H), 4.67 J =3.9 Hz, 2H), 4.80 2H), 4.85 (s, 0:0. 2H), 7.01 J 8.5 Hz', 1H), 7.43 J =1.8 Hz, 1H), 7.70 .0.00. (dd, J 1. 8, 8. 5 Hz, 1H).
SIMS 463 1).
Example 28 [(3-benzyl-4, 5, 6, 7-tetrahydrothielo3 2-c]pyridin-2yl)carbonyl]amino]acetyl-o-phelylene]dioxY]diacetic acid trifluoroacetate The title compound was prepared f rom the compounds of Referential Examples 3 and 9.
Di-t-butyl [[4-([[r(3-benzyl-5-t-butoxycarbonyl- 4 ,5, 6 ,7tetrahydrothieno[3, 2-c] pyridin-2-yl )carbonyl] amino] acetyl-l-odioxy] diacetate 1H-NMR (CDCl 3 6: 1.48 9H), 1.50 9H), 2.84 (brs, 2H), 3.83 (brs, 2H), 4.23 2H), 4.31 2H), 4.65 2H), 4.82 J 4.4 Hz, 2H), 6.84 J 8.5 Hz, 1H), 6.88 (brs, 1H), 7. 17 7.26 (in, 5H), 7.49 J 1. 8 Hz, 1H), 7. 60 (dd, J 1. 8, 8.5 Hz, 1H).
SIMS 751 1).
Title compound 1H-NMR (DMSO-d 6 5: 3.01 (brs, 2H), 3.88 (brs, 2H), 4.25 (brs, 2H), 4.68 J 5.4 Hz, 2H), 4.73 2H), 4.76 2H), 4.80 2H), 7.00 J 8.5 Hz, 1H), 7.17 7.29 (in, 5H), 7.43 (s, 1H), 7.68 J 8.5 Hz, 1H).
SIMS 539 1).
Example 29 5,6, 7-tetrahydrothlefo[ 3. 2-clpyrnidifl-2yl)carbonyllaminolacetyl-o-phelylefe]diOXY]diacetic acid trifluoroacetate The title compound was prepared f rom the compounds of Referential Examples 4 and 9. This compound is identical with the compound of Example 13.
Di-t-butyl [[4-[[[(5-t-butoxycarbonyl-4,5,6,7tetrahydrothienoll3,2-c]pyridin- 2 -yl )carbonyllaminolacetyl]-ophenylene] dioxy] diacetate IH-NMR (CDCl 3 6: 1.48 9H), 1.49 9H), 1.50 9H), 2.72 (brs, 2H), 3.69 (brs, 2H), 4. 64 (brs, 2H) 4. 67 2H) 4. 70 s, 2H), 4.84 J =4 Hz, 2H), 6.85 J 9 Hz, 1H), 6.97 7.02 (in, 1H), 7.33 1H), 7.51 J 9 Hz, 1H), 7.63 (dd, J 2.9 Hz, 1H).
SIMS 660 (M Title compound 0 *HNM
(DS*
1
HNMR(DMO-
6 6: 2.81 (brs, 2H), 3.23 (brs, 2H), 4.25 (in, 2H), 6.91 J 8 Hz, 1H), 7.40 1H), 7.58 1H), 7.62 J =8 Hz, 1H), 8.83 (brs, 1H); peaks corresponding to 6H overlap ~s with thesolvent peak.
FDMS 449 1).
Example 6, 7,8-tetrahydro-4H-thielo[3. 2-cl azepin-2yl)carbonyl] amino] acetyl] -o-phenylefle]dloxy]diacetic acid trifluoroacetate The title compound was prepared from the compounds of Referential Examples 5 and 9.
Di-t-butyl [[4-[[[(5-t-butoxycarbonyl-5,6,7,8-tetrahydro- 4H-thieno[3, 2-c] azepin-2-yl )carbonyl]aino] acetyl] ophenylene] dioxy] diacetate 'H-NMR (CDCl 3 CD 3 0D) 8: 1.37 1.55 (27H), 1.88 2H), 2.75 3.10 (in, 3H), 3.64 3.79 (mn, 2H), 4.32 4.43 (in, 2H), 4.67 (s, f* «o es..
9 a 4* -00a p.* a o*o* a.* 2H), 4.69 2H), 4.78 4.87 2H), 6.89 J 8.3 Hz, 1H), 7.40 7.50 1H), 7.53 J 1.9 Hz, 1H), 7.65 7.74 (m, 1H), 7.86 J 8.3 Hz, 1H).
EIMS 674 (M 1).
Title compound 1H-NMR (DMSO-d 6 6: 1.87 2.02 2H), 2.95 3.13 2H), 3.70 3.76 2H), 4.24 2H), 4.51 4.86 6H), 6.98 (s, 1H), 7.42 1H), 7.51 7.76 2H), 8.75 (brs, 1H).
FDMS 463 1).
Example 31 8-tetrahydro-4H--thieno[ 2 ,3-d]azepin-2yl)carbonyl]amino]acetyl]-o-phenylene]dioxy]diacetic acid trifluoroacetate 15 The title compound was prepared from the compounds of Referential Examples 6 and 9.
Di-t-butyl [[4-[[[(5-t-butoxycarbonyl-5,6,7,8-tetrahydro- 4H-thieno[2,3-d]azepin-2-yl)carbonyl]amino]acetyl]-ophenylene]dioxy]diacetate 1H-NMR (CDC1 3 6: 1.47 1.54 (27H), 2.79 3.06 4H), 3.49 3.70 4H), 4.67 2H), 4.70 6H), 4.84 J 10.3 Hz, 2H), 6.85 J 8.4 Hz, 1H), 7.30 1H), 7.50 J 1.9Hz, 1H), 7.63 (dd, J 1.9, 8.4 Hz, 1H).
EIMS 463 (M 1).
Title compound 1H-NMR (DMSO-d 6 6: 2.85 2.39 4.57 4.86 6H), 6.99 J 8.4 Hz, 1H), 7.42 1H), 7.58 1H), 7.67 J 8.4 Hz, 1H), 8.69 (brs, 1H).
FDMS 463 1).
Example 32 [[4-[N-[(4,5,6,7-tetrahydrothieno[3,2-c]pyridin-2yl)carbonyl]methyl]carbamoyl] -o-phenylene]dioxy]diacetic acid trifluoroacetate The title compound was prepared from the compounds of Referential Examples 8 and Di-t-butyl [[4-[N-(5-t-butyl-4,5,6,7-tetrahydrothieno [3,2-clpyridin-2-yl)methyl Icarbonlyl] -o-phefylefe] dioxy]diacetate IH-NMR (ODC1 3 6: 1.48 9H), 1.49 9H), 1.50 9H), 2.92 (brt, 2H), 3.75 (brt, 2H), 4.53 2H), 4.66 4H), 4.80 (d, 1 3.9 Hz, 2H), 6.85 J =7.8 Hz, 1H), 7.06 J =3.9 Hz, 1H), 7.43 1H), 7.56 1H), 7.42 (dd, J 2.0, 7.8 Hz, 1H).
FDMS 661 1).
Title compound IH-NMR (DMSO-d 6 6: 3.10 J 5.3 Hz, 2H), 3.43 J 5.3 Hz, 2H), 4.23 2H), 4.60 J 5.3 Hz, 2H), 4.73 2H), 4.77 2H), 6.97 J 8.4 Hz, 1H), 7.40 J 1.9 Hz, 1H), 7.92 1H), 8.82 1r 5.3 Hz, 1S).
Example 33 too 15 Diethyl [[4-[[2-[(4,5,6,7-tetrahydrothielo[3,2-c]pyridil-2yl)carbonyl]amino]ethyl]-o-phelylefle]dioxyldiacetate 0 trifluoroacetate Th~e title compound was prepared from the compounds of *.Referential Examples 1 and Diethyl [[4-[[2-[(5-t-butoxycarbonyl-4,5,6,7tetrahydrothieno[3, 2-c] pyridin-2-yl )carbonyll amino]ethyl] -oopt*.: phenylene]dioxyldiacetate H-NMR (CDCl 3 1 :12 i,6) 1.48 9H), 2.81 (mn, 4H), 3.58 (in, 2H), 3.70 (mn, 2H), 4.22 (mn, 4H), 4.42 2H), 4.67 2H), 4.68 2H), 6.75 7.17 (in, 4H).
Title compound IH-NMR (CD 3 OD) 6: 1.27 J 7.0 Hz, 3H), 1.28 J 7.0 Hz, 3H), 2.81 (mn, 2H), 3.17 (mn, 2H), 3.54 (mn, 4H), 4.22 (mn, 4H), 4.28 4H), 4.70 2H), 4.71 2H), 6.82 (dd, J 3.6, 8.3 Hz, 1H), 6.86 J 3.6 Hz, 1H), 6.88 J 8.3 Hz, 1H), 7.38 (s, 1H).
Example 34 Diethyl [[4-[[[(4,5,6,7-tetrahydrothieno[3,2-c]pyridin2> yl)carbonyl]ainino]acetyl]-o-pheflylefeldioxyldiacetate tri fluoroacetate The title compound was prepared from the compounds of Referential Examples 1 and 21.
Diethyl [[4-[[2-[(5-t-butoxycarbonyl-4,5,6,7tetrahydrothieno[ 3 ,2-c]pyridin-2-yl)carbonyl]amino]acetyl]-ophenylene]dioxy]diacetate IH-NMR (CDC1 3 6: 1.28 1.32 6H), 1.49 9H), 2.87 (brs, 2H), 3.73 (brs, 2H), 4.25 4.31 4H), 4.49 (brs, 2H), 4.78 2H), 4.81 2H), 4.84 J 4 Hz, 2H), 6.89 J 8 Hz, 1H), 6.98 7.03 1H), 7.32 1H), 7.55 J 2 Hz, 1H), 7.65 (dd, J 2.8 Hz, 1H), EIMS 604 (M Title compound 1H-NMR (CD 3 0D) 6: 1.29 J 7 Hz, 6H), 3.20 J 6 Hz, 2H), 3.58 J 6 Hz, 2H), 4.25 J 7 Hz, 2H), 4.26 J 7 Hz, 2H), 4.79 2H), 4.82 4H), 7.05 J 8 Hz, 1H), 7.53 1H), 7.60 J 2 Hz, 1H), 7.74 (dd, J 2.8 Hz, 1H).
Example [4-[[N-[3-(piperidin-4-yl)propionyl]amino]acetyl]-o- 20 phenylene]dioxy]diacetic acid trifluoroacetate The title compound was prepared from 3-(1-tbutoxycarbonylpiperidin-4-yl)propionic acid and the compound of Referential Example 9.
Di-t-butyl [[4-[[N-[3-(l-t-butoxycarbonylpiperidin-4- 25 yl)propionyl]amino]acetyl] -o-phenylene]dioxy]diacetate 1H-NMR (CDC1 3 6: 1.05 1.18 2H), 1.45 9H), 1.48 (s, 9H), 1.50 9H), 1.58 1.70 5H), 2.30 2.37 2H), 2.62 2.72 2H), 4.02 4.17 2H), 4.66 2H), 4.69 4H), 6.53 (brs, 1H), 6.83 J 9 Hz, 1H), 7.48 1H), 7.60 (d, J 9 Hz, 1H).
FDMS 634 (M Title compound 1H-NMR (DI0) 6: 1.32 1.44 2H), 1.53 1.67 3H), 1.88 1.98 2H), 2.34 2.45 2H), 2.90 3.02 2H), 3.34 3.47 2H), 4.65 2H), 4.84 2H), 4.88 2H), 7.04 (d, J 9 Hz, 1H), 7.45 1H), 7.66 J 9 Hz, 1H).
SIMS 423 (M 1).
Example 36 [[4-[[N-[4-(piperidin-4-yl)butyryl]amino]acetyl]-ophenylene]dioxy]diacetic acid trifluoroacetate The title compound was prepared from 4-(l-tbutoxycarbonylpiperidin-4-yl)butyric acid and the compound of Referential Example 9.
Di-t-butyl [[4-[[N-[4-(l-t-butoxycarbonylpiperidin-4yl)butyryl]amino]acetyl]-o-phenylene]dioxy]diacetate 1 H-NMR (CDC13) 6: 1.04 2H), 1.28 2H), 1.45 9H), 1.48 9H), 1.50 9H), 1.60 1.72 5H), 2.30 2H), 2.67 2H), 4.07 (brs, 1H), 4.66 2H), 4.69 2H), 4.70 (s, 2H), 6.54 (brs, 1H), 6.83 J 8.5 Hz, 1H), 7.48 J 2.1 Hz, 1H), 7.60 (dd, J 2.1, 8.5 Hz, 1H).
FDMS 649 (M 1).
Title compound SH-NMR 6: 1.25 1.40 4H), 1.52 1.68 3H), 1.93 J 11.1 Hz, 2H), 2.38 2H), 2.96 J =12.5 Hz, 2H), 20 3.40 J 12.5 Hz, 2H), 4.66 2H), 4.85 2H), 4.89 (s, 2H), 7.06 J 8.3 Hz, 1H), 7.48 J 1.9 Hz, 1H), 7.70 J 1.9, 8.3 Hz, 1
H).
s Example 37 25 [[4-[[N-[2-(piperidin-4-yl)acetyl]-N-methylamino]acetyl]-ophenylene]dioxy]diacetic acid trifluoroacetate The title compound was prepared from 2-(l-tbutoxycarbonyipiperidin-4-yl)acetic acid and the compound of Referential Example 11.
Di-t-butyl [[4-[[N-[2-(l-t-butoxycarbonylpiperidin-4yl)acetyl]-N-methylamino]acetyl]-o-phenylene]dioxy]diacetate FDMS 634 Title compound 1H-NMR 6: 1.48, 1.51 2H), 1.92, 2.00 2H), 2.10 (m, 1H), 2.28, 2.54 J 6.9 Hz, 2H), 2.96, 3.15 3H), 3.10 (m, 2H), 3.42 2H), 4.84 2H), 4.85 2H), 4.88 2H), 7.04, 7.12 J 8.6 Hz, 1H), 7.45, 7.49 1H), 7.67, 7.72 J 8.6 Hz, 1H).
Example 38 3- piperidin-4-yl )propionyl] amino] acetyllphenylpropionic acid trifluoroacetate The title compound was prepared form 3-(1-tbutoxycarbonylpiperidin-4-yl)propionic acid and the compound of Referential Example 12.
Diphenylmethyl 3- l-t-butoxycarbonylpiperidin-4yl )propionyl] amino] -acetyl] phenyipropionate IH-NMR (CDCl 3 6: 1.06 1.18 (in, 2H), 1.15 9H), 1.60 1.73 (mn, 4H), 2.34 3 8 Hz, 2H), 2.61 2.73 (in, 2H), 2.78 (t, i 8 Hz, 2H), 3.05 3 8 Hz, 2H), 4.02 4.15 (in, 1H), 4.71 J 4 Hz, 2H), 6.52 6.56 (mn, 1H), 6.87 1H), 7.24 7.34 (mn, 12H), 7.85 J 8 Hz, 1H).
SIMS 613 Title compound 'H-NMR (CDCl 3 6: 1.34 1.46 (in, 2H), 1.60 1.70 (in, 2H), 1.98 J 14 Hz, 2H), 2.44 J 7 Hz, 2H), 2.73 2.81 (mn, 2H), 2.93 -3.08 (mn, 4H), 3.43 J 13 Hz, 2H), 4.75 2H), 7.47 3 8 Hz, 2H), 7.95 J 8 Hz, 2H).
SIMS 347 1).
Example 39 4- 2- 5, 6, 7 -tet rahyd rothjen-o F 3, 2 -c pyr idin -2 yl )carbonyl]ainino]ethyl]phefloxyacetic acid trifluoroacetate The title compound was prepared f rom the compounds of Referential Examples 1 and 14.
Ethyl [(5-t-butoxycarbonyl-4, 5,6, 7-tetrahydrothieno 2-c]pyridin-2-yl )carbonyllaminolethyl]phenoxyacetate This compound was prepared according to the method described in Example 1 'H-NMR (D 2 0) 6: 1.30 J 7.2 Hz, 3H), 1.48 9H), 2.84 (t, 1 6.8 Hz, 4H), 3.63 3 6.8 Hz, 2H), 3.71 (brs, 2H), 4.28 J 7.2 Hz, 2H), 4.45 (brs, 2H), 4.61 2H), 6.87 J 8.6 Hz, 2H), 7.11 1H), 7.14 J 8.6 Hz, 2H).
EIMS 488 To the solution of the compound prepared in (250 mg) in ethanol was added IN sodium hydroxide (2.6 ml), and the mixture was stirred under ice-cooling for 3 hours. After the reaction mixture was concentrated under reduced pressure, ethyl acetate and water were added. The mixture was acidified with 1N hydrochloric acid. The organic layer was washed with water, dried over anhydrous magnesium sulfate and concentrated under reduced pressure to give 214 mg of 4-[2-[[(5-t-butoxycarbonyl-4,5,6,7tetrahydrothieno[3,2-c]pyridin-2-yl)carbonyl]amino]ethyl] phenoxyacetic acid (yield, 91%).
1H-NMR (CDC1 3 6: 1.49 9H), 2.92 4H), 3.45 (brs, 2H), 3.73 2H), 4.53 (brs, 2H), 4.68 2H), 6.87 (brs, 2H), 7.10 :i"15 (brs, 2H), 7.27 1H).
FDMS 461 1).
The title compound was prepared according to the method described in Example 1 1 H-NMR (CD 3 OD) 6: 2.92 J 7.2 Hz, 2H), 3.26 J 6.1 Hz, 20 2H), 3.60 J 7.2 Hz, 2H), 3.64 J 6.1 Hz, 2H), 4.37 (s, 2H), 4.70 2H), 4.97 2H), 6.95 J 8.3 Hz, 2H), 7.24 J 8.3 Hz, 2H), 7.47 1H).
FDMS 361 (M 1).
Example S[[4-[2-[N-[(4,5,6,7-tetrahydrothieno[3,2-c]pyridin-2yl)methyl]amino]ethyl]-o-phenylene]dioxy]diacetic acid trifluoroacetate To the solution of the compound prepared in Referential Example 7 (500 mg) in methanol (20 ml) were added the compound prepared in Referential Example 20 (822 mg) and sodium cyanoborohydride (235 mg), and the mixture was stirred at room temperature for 16 hours. After the reaction mixture was concentrated under reduced pressure, the residue was purified by column chromatography on silica gel. Elution with chloroform methanol 10 1 gave 530 mg of diethyl butoxycarbonyl-4,5,6,7-tetrahydrothieno[3,2-c]pyridin-2yl)methyl]amino]ethyl]-o-phenylene]dioxy]diacetate (yield, 49.1%).
1 H-NMR (CDC1 3 6: 1.28 6H), 1.48 9H), 2.80 6H), 3.78 (brs, 2H), 3.89 2H), 4.24 4H), 4.41 (brs, 2H), 4.69 (m, 4H), 6.57 1H), 6.75 6.85 3H).
To the solution of the compound prepared in (78 mg) in methanol (1 ml) was added lN sodium hydroxide (5 ml), and the mixture was stirred at room temperature for 1 hour and then concentrated under reduced pressure. After anisole (1 ml) and trifluoroacetic acid (5 ml) were added to the residue, the mixture was stirred for 3 hours. The-reaction mixture was taken into isopropyl ether. Deposits were collected by filtration, dissolved in water, adsorbed on HP-20, washed with water, and 15 eluted with water acetone 10 1 to give 50 mg of the title compound (yield, 87.9%).
1 H-NMR (D 2 0 DC1) 6: 2.81 2H), 3.01 2H), 3.16 2H), 3.42 2H), 4.13 2H), 4.24 2H), 4.67 4H), 6.82 (m, 4H).
Example 41 [[4-[2-[N-[(4,5,6,7-tetrahydrothieno[3, 2-c]pyridin-2-yl)methyl]- N-acetylamino]ethyl]-o-phenylene]dioxy]diacetic acid trifluoroacetate To the solution of the compound prepared in Example 23 (309 mg) in pyridine (2 ml) was added acetic anhydride (1 ml), and the mixture was stirred at room temperature for 3 hours.
The reaction mixture was dissolved in ethyl acetate, washed with water, dried over anhydrous magnesium sulfate. After the solvents were eveporated, the residue was purified by column chromatography on silica gel, and the fraction eluted with ethyl acetate n-hexane 3 1 gave 163 mg of diethyl t-butoxycarbonyl-4,5,6,7-tetrahydrothieno[3,2-c]pyridin-2yl)methyl]-N-acetylamino]ethyl]-o-phenylene]dioxy]diacetate (yield, 49.2%).
1H-NMR (CDC1 3 6: 1.28 J 7.2 Hz, 3H), 1.29 J 7.2 Hz, 3H), 1.48 9H), 1.92, 2.17 (double s, 3H), 2.76 4H), 3.49 2H), 3.69 (brs, 2H), 4.25 J 7.2 Hz, 2H), 4.26 J 7.2 Hz, 2H), 4.34, 4.57 (double s, 2H), 4.41 (brs, 2H), 4.69 (s, 2H), 4.70 2H), 6.53 1H), 6.64 6.84 3H).
Reaction was performed according to the method described in Example 23 to give the title compound.
1H-NMR (D 2 0 DC1) 6: 1.46, 1.90 (double s, 3H), 2.54 2H), 2.81 (brs, 2H), 3.27 2H), 3.37 2H), 3.96 2H), 4.27 2H), 4.46 2H), 4.49 2H), 6.41 6.63 4H).
Example 42 Di-(5-methyl-2-oxodioxol-4-yl)methyl tetrahydrothieno[3,2-c]pyridin-2-yl).carbonyl]amino]acetyl]-ophenylene]dioxy]diacetic acid trifluoroacetate To the solution of the compound prepared in Example 17 "15 (2 g) in THF (50 ml) was added an aqueous solution (50 ml) of sodium hydroxide (240 mg), and the mixture was stirred at room temperature for 20 minutes. After THF was evaporated under reduced pressure, the concentrated reaction mixture was acidified to pH 3.5 with 5N hydrochloric acid. Crystals deposited were collected by filtration and dried to give 1.21 g of butoxycarbonyl-4,5,6,7-tetrahydrothieno[ 3 ,2-c]pyridin-2yl)carbonyl]amino]acetyl]-o-phenylene]dioxy]diacetic acid.
IH-NMR (D20) 6: 1.52 9H), 2.90 (brs, 2H), 3.74 (brs, 2H), 4.50 (brs, 2H), 4.61 2H), 4.66 2H), 4.86 2H), 7.01 J 9 Hz, 1H), 7.44 J 2 Hz, 1H), 7.48 1H), 7.74 (dd, J 2 Hz, 9 Hz, 1H).
To the solution of the compound prepared in (100 mg) in DMF (3 ml) were added 4-bromomethyl-5-methyl-2-oxodioxole (104 mg) and cesium fluoride (82 mg), and the mixture was stirred at room temperature for 19 hours. Water (10 ml) was added to the reaction mixture. The resulting mixture was extracted with ethyl acetate, washed with water and dried over anhydrous magnesium sulfate. After the solvents were evaporated, the residue was purified by column chromatography on silica gel, and the fraction eluted with chloroform methanol 20 1 gave 122 mg of methyl-2-oxodioxol-4-yl)methyl 4, 5 6 7-tetrahydrothieno[ 3, 2-c] pyridin-2te..
a 4 4
S.
yl )carborlyl] amino] acetyl]l -o-phenyleneldioxy]diacetate.
IH-NMR (CDCl 3 6: 1.50 9H), 2.19 6H), 2.88 (brs, 2H), 3.74 (brs, 2H), 4.51 (brs, 2H), 4.83 2H), 4.85 (di, J 4 Hz, 2H), 4.86 2H), 5.02 2H), 6.90 J 8 Hz, 1H), 6.97 7.01 (brs, 1H), 7.35 1H), 7.51 J 2 Hz, 1H), 7.66 (cid, J 2 Hz, 8 Hz, 1H).
SIMS 773 1) The compound prepared in was subjected to reaction according to the method described in Example 1 to give the title compound.
1 H-NMR (DI0) 6: 2.13 3H), 2.15 3H), 3.25 (brs, 2H), 3.60 3.64 (in, 2H), 4.37 (brs, 2H), 4.97-(s, 2H), 5.02 2H), 5.11 4H), 7.07 (di, J 8 Hz, 1H), 7.48 (ci, J 2 Hz, 1H), 7.55 (s, 1H), 7.74 (dci, J 2 Hz, 8 Hz, 1H).
15 FDMS 673 1).
Example 43 phenyleneldioxyldiacetic acid 1/2 sulfate 20 The compound prepared in Example 18 (200 mg) was dissolved in concentrated sulfuric acid (1 ml). The solution was stirred at room temperature for 2 hours. Crystals deposited were collected by filtration, washed with water and dried to give 76 mg of the title compound (yield, 'H-NMR (D 2 0) 6: 1.28 -1.45 (in, 2H), 1.46 -1.68 (mn, 3H), 1.82 1.95 (in, 2H), 2.58 -2.70 (in, 2H), 2.85 -2.88 (in, 2H), 3.33 3.47 (in, 2H), 4. 55 2H), 4. 58 s, 2H) 6.74 (ci, J 9 Hz, 1H), 6.79 1H), 6.91 (di, J 9 Hz, 1H), 6.99 1H).
FDMS 405 1).
Example 44 5, 6,7-tetrahydrothiela[3, 2-c] pyridin- 2 yl )carbonylamino]acetyl]pyriciifl5-yloxyacetic acid tri fluoroacetate To the solution of oxalyl chloride (10 iil) in dichioromethane (0.5 ml) was added the solution of dimethylsulfoxide (17 pl) in dichloromethane (0.5 ml) at -78-C, and the mixture was stirred for 4 minutes. The solution of the compound prepared in Example 21 (58 mg) in dichloromethane (1 ml) was added to this solution. The mixture was stirred for 15 minutes. Triethylamine (76 pl) was further added to the mixture to continue the reaction at -78"C for 15 minutes and at O'C for 30 minutes. A saturated aqueous ammonium chloride solution was added to the mixture to terminate the reaction. The mixture was extracted with ethyl acetate, washed with water and brine and dried over anhydrous magnesium sulfate. After the solvents were evaporated under reduced pressure, the residue was purified by column chromatography on silica gel. A fraction eluted with ethyl acetate n-hexane (1 2) gave 12 mg of tbutyl 2-[[(5-t-butoxycarbonyl-4,5,6,7-tetrahydrothieno[ 3 2 1H-NMR (CDCl 3 6: 1.49 9H), 1.50 9H), 2.87 2H), 3.73 n(m, 2H), 4.50 2H), 4.66 2H), 5.09 J 4.7 Hz, 2H), 6.90 J 4.7 Hz, 1H), 7.27 (dd, J 2.8, 8.9 Hz, 1H), 7.32 1H), 8.07 J 8.9 Hz, 1H), 8.36 J 2.8 Hz, 1H).
The compound prepared in was treated in the same manner as in Example 1 to give 10 mg of the title compound (yield, 73%).
1H-NMR (CD 3 OD) 6: 3.20 J 5.8 Hz, 2H), 3.31 2H), 3.58 J 5.8 Hz, 2H), 4.32 2H), 4.98 2H), 6.61 1H), 7.48 (dd, J 2.8, 8.9 Hz, 1H), 8.05 J 8.9 Hz, 1H), 8.40 J 2.8 Hz, 1H).
SIMS 376 1).
Example [[4-[[2-[(4,5,6,7-tetrahydrothieno[3,2-c]pyridin-2yl)carbonyl]amino]-l-hydroxyethyl]-o-phenylene]dioxy]diacetic acid trifluoroacetate To the solution of the compound prepared in Example 18 (634 mg) in ethanol (6 ml) was added sodium borohydride (52 mg), and the mixture was stirred at room temperature for 3 hours.
The reaction mixture was extracted with ethyl acetate, washed with water and dried over anhydrous magnesium sulfate. After the solvents were evaporated, the residue was purified by column chromatography on silica gel, and the fraction eluted with ethyl acetate n-hexane (2 1) gave 370 mg of di-t-butyl t-butoxycarbonyl-4,5,6,7-tetrahydrothieno[ 3 2 -c]pyridin- 2 yl)carbonyl]amino]-1-hydroxyethyl]-o-phenylene]dioxy]diacetate (yield, 58.2%).
1H-NMR (CDC1 3 6: 1.47 (27H), 2.85 (brs, 2H), 3.18 (brs, 1H), 3.43 1H), 3.72 (brs, 2H), 3.82 1H), 4.45 2H), 4.59 2H), 4.60 2H), 4.86 1H), 6.35 (brs, 1H), 6.82 J 8.0 Hz, 1H), 6.92 2H), 7.20 1H).
The compound prepared in was treated in the same manner as in Example 1 to give the title compound.
1 H-NMR (DMSO-D 6 6: 3.05. 2H), 3.74 1H), 4.18 2H), 4.60 4.75 4H), 6.77 6.91 4H); peaks corresponding to 15 4H overlap with the peaks of the solvent.
Example 46 7-tetrahydrothieno [3,2-c]pyridin-2yl)carbonyl]thiazol-4-yl]phenoxyacetic acid trifluoroacetate 4'-(4-methoxybenzyl)oxyacetophenone was subjected to reaction according to the method described in Referential Example 15 to give 4'-(p-methoxybenzyl)oxy-2-bromoacetophenone.
-NMR (CDC1 3 6: 3.82 3H), 4.40 2H), 5.07 2H), 6.93 J 8.9 Hz, 2H), 7.02 J 8.9 Hz, 2H), 7.35 J 8.9 Hz, 2H), 7.97 J 8.9 Hz, 2H).
SIMS 335 (M 1).
Phosphorus pentasulfide (198 mg) and formamide (2 ml) were mixted and stirred at room temperature overnight. The solution of the compound prepared in (1.34 g) in THF (5 ml) was added to this mixture. The resulting mixture was stirred for 1 hour. After saturated sodium hydrogen carbonate and water were added, the mixture was extracted with ethyl acetate, washed with water and dried over anhydrous magnesium sulfate. The solvents were evaporated. Crystals thus obtained were washed with ether and dried to give 689 mg of 4- methoxybenzyl)oxyphenyl]thiazole (yield, 58%).
1H-NMR (CDC13) 6: 3.82 3H), 5.04 2H), 6.92 J 8.6 Hz, 2H), 7.03 J 8.6 Hz, 2H), 7.38 J 8.6 Hz, 2H), 7.40 J 1.9 Hz, 1H), 7.86 J 8.6 Hz, 2H), 8.85 J 1.9 Hz, 1H).
EIMS 297 The solution of the compound prepared in (150 mg) in THF (3 ml) was cooled to -78C, and n-butyl lithium (1.5 M, nhexane solution, 0.33 ml) was added dropwise. The mixture was stirred at the same temperature for 10 minutes. To this solution was added dropwise the solution of the compound prepared in Referential Example 7 (133 mg) in THF (2 ml), and the mixture was stirred at -78'C for 30 minutes. The reaction mixture was diluted with ether, washed with water, and dried over anhydrous magnesium sulfate. After the solvents were evaporated, the residue was 15 purified by column chromatography on silica gel with an eluent.
system (n-hexane ethyl acetate 2 1) to give 125 mg of 2-[1- (5-t-butoxycarbonyl-4,5,6,7-tetrahydrothieno[3,2-c]pyridin-2-yl)l1-hydroxymethyl]-4-[4-(p-methoxybenzyl]oxyphenyl]thiazole (yield, 44%).
1H-NMR (CDC1 3 6: 1.47 9H), 2.81 (brs, 2H), 3.63 (brs, 1H, disappeared by 3.69 (brs, 2H), 3.81 3H), 4.43 (brs, 2H), 5.03 2H), 6.23 J 4.0 Hz, 1H), 6.82 1H), 6.92 J Hz, 2H), 7.01 J 8.5 Hz, 2H), 7.34 1H), 7.37 J 8.5 Hz, 2H), 7.82 J 8.5 Hz, 2H).
EIMS 564 To the solution of the compound prepared in (290 mg) in dichloromethane (6 ml) was added under ice-cooling pyridinium chlorochromate (220 mg), and the mixture was stirred under icecooling for 2 hours and at room temperature for 1 hour. The reaction mixture was diluted with ethyl acetate. Insolubles were removed by filtration through celite. The filtrate was washed with saturated sodium hydrogen carbonate and water and dried over anhydrous magnesium sulfate. After the solvents were evaporated, the residue was purified by column chromatography on silica gel with the eluent system of n-hexane ethyl acetate 2 1 to give 174 mg of 2-[(5-t-butoxycarbonyl- 4 ,5, 6 7 tetrahydrothieno[3,2-c]pyridin-2-yl)carbonyl]-4-[ 4 methoxybenzyl)oxyphenyl]thiazole (yield, 60.2%).
1H-NMR (CDC1 3 6: 1.51 9H), 2.97 (brs, 2H), 3.77 (brs, 2H), 3.83 3H), 4.59 (brs, 2H), 5.07 2H), 6.94 J 8.6 Hz, 2H), 7.09 J 8.6 Hz, 2H), 7.39 J 8.6 Hz, 2H), 7.71 (s, 1H), 7.92 J 8.6 Hz, 2H), 8.34 (brs, 1H).
SIMS 563 (M 1).
The compound prepared in (170 mg), anisole (0.2 ml) and trifluoroacetic acid (2 ml) were reacted at room temperature for 30 minutes. Diisopropyl ether (10 ml) was added to the reaction mixture, and precipitates was collected by filtration.
Precipitates thus obtained (137- mg) was dissolved in dichloromethane (2 ml). Di-t-butyl dicarbonate (72 mg), triethylamine (0.15 ml) and DMF (0.5 ml) were added to the solution. The mixture was stirred at room temperature for 2 hours. The reaction mixture was diluted with ethyl acetate, washed with water and dried over anhydrous magnesium sulfate.
After the solvents were evaporated, the residue was purified by column chromatography on silica gel with an eluent system of n- 20 hexane ethyl acetate 3 1 to give 125 mg of butoxycarbonyl-4,5,6,7-tetrahydrothieno[3,2-c]pyridin-2yl)carbonyl]-4-(p-hydroxyphenyl]thiazole (yield, 94%).
H-NMR (CDC1 3 6: 1.51 9H), 2.98 (brs, 2H), 3.78 (brs, 2H), 4.59 (brs, 2H), 6.96 J 8.5 Hz, 2H), 7.69 1H), 7.86 (d, J 8.5 Hz, 2H), 8.34 (brs, 1H).
EIMS 442 To the solution of the compound prepared in (119 mg) in DMF (2 ml) were added potassium carbonate (74 mg) and t-butyl bromoacetate (0.047 ml), and the mixture was stirred at 60 70
C
for 1.5 hours. The reaction mixture was diluted with ethyl acetate, washed with water, and dried over anhydrous magnesium sulfate. After the solvents were evaporated, the residue was purified by column chromatography on silica gel with an eluent system of n-hexane ethyl acetate 3 1 to give 129 mg of tbutyl 4-[[2-(5-t-butyloxycarbonyl-4,5,6,7-tetrahydrothieno[ 3 2 c]pyridin-2-yl)carbonyl]thiazol-4-yl]phenoxyacetate (yield, 86%).
1H-NMR (CDC1 3 6: 1.50 9H), 1.51 9H), 2.98 (brs, 2H), 3.78 (brs, 2H), 4.59 4H), 7.02 J 8.6 Hz, 2H), 7.72 (s, 1H), 7.92 J 8.6 Hz, 2H), 8.35 (brs, 1H).
EIMS 556 (M The compound prepared in was treated in the same manner as described in Example 1 to give the title compound.
1H-NMR (DMSO-d 6 D,0) 6: 3.25 2H), 3.51 2H), 4.35 (brs, 2H), 4.76 2H), 7.08 J= 8.7 Hz, 2H), 8.05 J 8.7 Hz, 2H), 8.41 8.43 1H).
FDMS 400 Example 47 5,6,7-tetrahydrothieno[3, 2-c] pyridin-2yl)carbonyl]thiazol-2- yl]phenoxyacetic acid trifluoroacetate.
15 To the solution of 4-(p-methoxybenzyl)oxybenzonitrile (7.18 g) in pyridine (100ml) was added triethylamine (20 ml), and hydrogen sulfide gas was streamed 30 minutes. The mixture was stirred at room temperature for 3 hours followed by 50 C overnight. After the solvent was evaporated under reduced 0 pressure, the residue was taken into ether, and crystals were collected by filtration and dried to give 8.05 g of 4-(pmethoxybenzyl)oxythiobenzamide (yield, 98%).
EIMS 273 To the solution of the compound prepared in (273 mg) in DMF (3 ml) was added chloroacetoaldehyde (40% aqueous solution, 0.3 ml), and the mixture was stirred at 60 C for 3 hours. The reaction mixture was diluted with ethyl acetate, washed with water and dried over anhydrous magnesium sulfate.
After the solvent was evaporated, the residue was taken into ether, and crystals were collected by filtration and dried to give 203 mg of 2-[4-(p-methoxybenzyl)oxyphenyl]thiazole (yield, IH-NMR (CDC1 3 6: 3.82 3H), 5.04 2H), 6.93 J 8.7 Hz, 2H), 7.03 J 8.7 Hz, 2H), 7.25 J 3.3 Hz, 1H), 7.37 J 8.7 Hz, 2H), 7.80 J 3.3 Hz, 1H), 7.91 J 8.7 Hz, 2H).
EIMS 297 The compound prepared in was subjected to reaction according to the method described in Example 29 to give the title compound.
1 H-NMR (DMSO-d 6 6: 3.18 2H), 3.50 2H), 4.29 (brs, 2H), 4.80 (brs, 2H), 7.09 J 8.8 Hz, 2H), 8.02 J 8.8 Hz, 2H), 8.06 1H), 8.68 1H).
Referential Example 22 6-t-butoxycarbonyl-4,5,6;7-tetrahydrothiazolo[5,4-c]pyridin- 2 carboxylic acid 6-ethoxycarbonyl-4, 5,6, 7-tetrahydrothiazolo[5, 4 c]pyridine The suspension of phosphorus pentasulfide (6.668 g, 1. 5 mmole) in formamide (25 ml) was stirred overnight at room temperature. The reaction solution was taken into water, extracted with ether and dried over anhydrous magnesium sulfate.
The solvents were evaporated to give thioformamide (3.964 g).
To the thioformamide this obtained was added 3-chloro-lethoxycarbonylpiperidin-4-one (5.141 g, 25 mmole) in ethanol e.
(100ml) prepared according to the method described in Tetrahedron, 39, 3767 (1983) and heated at reflux in the presence of Molecular Sieves 4A for 15 hours. The reaction mixture was *0O. cooled to room temperature. After a saturated aqueous sodium hydrogen carbonate solution was added to the mixture under icecooling, ethanol was evaporated under reduced pressure. The reaction mixture was extracted with chloroform, washed with brine and dried over anhydrous magnesium sulfate. The solvent was evaporated under reduced pressure to give 6.175 g of a crude product. The product was purified by column chromatography on silica gel (C-200 150 g, eluent: CHC13) to give 3.213 g of the title compound as a clear pale yellow oily product (yield, 60.5%).
IH-NMR (CDC1 3 6: 1.30 J 7.03 Hz, 3H), 2.88 3.00 2H), 3.74 3.87 2H), 4.20 J 7.03 Hz, 2H), 4.73 2H), 8.68 1H).
4 ,5,6,7-tetrahydrothiazolo[5,4-c]pyridine To the compound prepared in Referential Example 20 (a) (3.20 g, 15.6 mmole) was added 3.5N KOH (50 ml), and the mixture was heated at reflux 1 hour. The reaction mixture was cooled to room temperature, and concentrated hydrochloric acid was added under ice-cooling to adjust pH to 8 9. The reaction mixture was saturated by the addition of sodium chloride, then extracted with chloroform and dried over anhydrous magnesium sulfate. The solvent was evaporated under reduced pressure to give 1.488 g of the title compound as a clear pale yellow oily product (yield, which was used for the next reaction without purification.
6-t-butoxycarbonyl-4,5,6,7-tetrahydrothiazolo[5,4c]pyridine :i:15 To the solution of the compound prepared in Referential Example 20 (1.458 g, 10.4 mmole) in DMF (50 ml) were added triethylamine (2.2 ml, 15.8 mmole) and di-t-butyl dicarbonate (2.5 ml, 10.9 mmole) at room temperature. After the mixture was stirred at room temperature for 5 hours, IN HC1 was added under ice-cooling. The mixture was extracted with ethyl acetate, washed with saturated sodium hydrogen carbonate and brine in this sequence, dried over anhydrous magnesium sulfate. The solvent was evaporated under reduced pressure to give 2.585 g of a crude product. The product was purified by column chromatography on o 25 silica gel (C-200 100 g, hexane ethyl acetate 10 1 1) to give 1.493 g of the title compound as a pale yellow S: crystal (yield, 59.7%).
*"IH-NMR (CDC1 3 6: 1.49 9H), 2.84 3.03 2H), 3.66 3.85 2H), 4.68 2H), 8.68 1H).
EIMS 240 6-t-butoxycarbonyl-4,5,6,7-tetrahydrothiazolo[5,4c]pyridin-2-carboxylic acid To the solution of the compound prepared in Referential Example 20 (721.0 mg, 3.0 mmole) in THF (50 ml) was added dropwise a 1.6 M solution of n-butyl lithium in hexane (2.44 ml, 3.9 mmole) at -78'C under argon. After stirring the mixture for minutes, carbon dioxide was blown into the reaction mixture at 73 -78'C. After water and ether were added and the mixture was extracted with 5N sodium hydroxide, the mixture was acidified with concentrated hydrochloric acid to pH 4. The mixture was further extracted with chloroform, washed with brine and dried over anhydrous magnesium sulfate. The solvents were evaporated under reduced pressure to give 434.3 mg of the title compound as a brown crystal (yield, 50.9%).
1H-NMR (CDC1 3 6: 1.50 9H), 2.90 3.05 2H), 3.69 3.87 2H), 4.75 2H).
Example 48 [[4-[[[(4,5,6,7-tetrahydrothiazolo[5,4-c]pyridin-2yl)carbonyl]amino]-1-hydroxyethyl]-o-phenylene]dioxy]diacetic acid trifluoroacetate 15 Di-t-butyl [[4-[[[(6-t-butoxycarbonyl-4,5,6,7tetrahydrothiazolo[5,4-c]pyridin-2-yl)carbonyl]amino]-lhydroxyethyl]-o-phenylene]dioxy]diacetate To. a flask in which the compound prepared in Referential *Example 20 (137.9 mg, 0.485 mmole) and di-t-butyl amino-l-hydroxyethyl)-o-phenylene]dioxy]diacetate (209.5 mg, 0.485 mmole) were placed was added a solution of HOBT (72.2 mg, 0.534 mmole) in DMF (4.8 ml). Triethylamine (75 ml, 0.538 mmole) and l-ethyl-3-(3'-dimethylaminopropyl)carbodiimide (WSCI) (102.4 mg, 0.534 mmole) were added under ice-cooling to the flask. The mixture was stirred for 10 minutes and then heated up to room temperature. After stirring for 4.5 hours, HOBT (72.2 mg, 0.534 mmole) and WSCI (102.4 mg, 0.534 mmole) were added. The mixture was then stirred at room temperature for 20.5 hours. After water was added to the reaction mixture, the resulting mixture was extracted with ethyl acetate, washed with brine and dried over anhydrous magnesium sulfate. The solvent was evaporated under reduced pressure to give 398.2 mg of a crude product, which was purified thrice by column chromatography on silica gel with an eluent system of CHCl 3 MeOH 50 1 150 1 to give 152.6 mg of the title compound as a pale yellow crystal (yield, 47.4%).
1H-NMR (CDC1 3 6: 1.44 1.54 27H), 2.82 2.92 2H), 3.41 3.52 2H), 3.68 3.86 3H), 4.59 2H), 4.60 2H), 74 4.69 2H), 4.83 4.89 1H), 6.83 J 8.72 Hz, 1H), 6.91 6.97 2H).
EIMS 663 To the solution of the compound prepared in (115.3 mg, 0.174 mmole) in anisole (0.5 ml) was added trifluoroacetic acid (2.0 ml) at O'C. Crystals were deposited by the addition of isopropyl ether at O:C, collected by filtration, and lyophilized to give 82.1 mg of a crude product, which was purified on a LH column (50% methanol) to give 76.6 mg of the title compound as a colorless crystal (yield, 77.8%).
1H-NMR (DMSO-d 6 6: 2.86 3.06 2H), 3.40 3.89 4.25 4.50 2H), 4.55 4.70 4H), 6.68 6.98 3H).
Referential Example 23 15 t-Butyl [4-(aminoacetyl)-2-propyloxy]phenoxyacetate hydrochloride t-Buty [4-(azidoacetyl)-2-allyloxy]phenoxyacetate To the solution of the compound prepared in Referential Example 19 (500 mg, 1.63 mmole) in acetone (7 ml) were added potassium carbonate (270 mg) and allyl iodide (0.5 ml), and the 20 mixture was stirred at room temperature for 25 hours. The :o reaction mixture was diluted with ethyl acetate, washed with Swater and dried over anhydrous magnesium sulfate. After the inorganic salt was removed by filtration, the filtrate was concentrated under reduced pressure. The oily product thus 25 obtained was purified by column chromatography on silica gel with 5 an eluent system of chloroform ethyl acetate 20 1 to give S. 500 mg of the title compound as a pale yellow crystal (yield, 88%).
1H-NMR (CDC1 3 6: 1.48 9H), 4.50 2H), 4.67 2H), 4.68 4.70 2H), 5.30 5.35 1H), 5.42 5.50 1H), 6.04 6.13 1H), 6.49 J 8.21Hz, 1H), 7.47 (dd, J 2.06, 8.21 Hz, 1H), 7.53 J 2.06 Hz, 1H).
EIMS 347 Starting from 485 mg (1.40 mmole) of the compound prepared in the title compound was prepared as a pale yellow oily product (474 mg; yield, 94%) according to the method described in Referential Example 9 'H-NMR (CD 3 OD) 6: 1.08 J 7.49 Hz, 3H), 1.49 9H), 1.86 (in, J 7.49 Hz, 2H), 4.06 J 7.49 Hz, 2H), 4.52 2H), 4.74 2H), 6.97 (di, J 8.32 Hz, 1H), 7.58 J 2.22 Hz, 1H), 7.62 (dd, J 2.22 Hz, 8.32 Hz, 1H).
EIMS 323 Example 49 4, 5,6, 7-tetrahydroth2-eno[ 3 2 pyridin-2yl)carbonyl]aminoaCety1,]-2-propyloxyphenoxyacetic acid tri fluoroacetate t -B utyl1 4- 5-t-but(3xycarbonyl- 4 5,6,7tetrahydrothieno[3, 2-c]pyridin-2-yl)carboflyl]-aminoacetyl]- 2 propyloxyphefloxyacetate Starting from 355 mg (1.25 mmole) of the compound prepared in Referential Example 1 and 450mg of the compound prepared in Referential Example 26, the title compound was prepared a yellow foam product (580 mg; yield, 79%) according to the method described in Exam .e 9 H-NMR (CDCl 3 6: 1.08 J 7.49 Hz, 3H), 1.48 9H), 1.50 9H), 1.90 (in, J 7.49 Hz, 2H), 2.88 (brs, 2H), 3.74 (brs, 2H), 4.06 J 7.49 Hz, 2H), 4.51 (brs, 2H), 4.67 2H), 6. 82 3 8.60 Hz, lH), 7.04 (brs, 1H), 7.33 1H), 7. 55 (d, J 2.22 Hz, 1H), 7.60 (dd, J 2.22 Hz, 8.60 Hz, 1H).
ElMS 588 Starting from 500 mg (0.849 mmole) of the compound prepared in the ti-tle compound wa s prepared as a pale yellow foam product (389 mg; yield, 84%) according to the method described in Example 9 IH-NMR (CD 3 OD) 6: 1.09 3 7.21 Hz, 3H), 1.87 (in, J 7.21 Hz, 2H), 3.21 J 6.10 Hz, 2H), 3.58 J3 6.10 Hz, 2H), 4.06 3 7.21 Hz, 2H), 4.32 (brs, 2H), 4.80 2H), 4.82 (s, 2H), 7.00 J 8.60 Hz, 1H), 7.54 1H), 7.59 1H), 7.68 J 8.60 Hz, 1H).
FDMS 433 1).
Ir Referential Example 24 t-Butyl [4-(aminoacetyl)-2-hydroxy]phenoxyacetate hydrochloride Starting from 500 mg (1.63 mmole) of the compound prepared in Referential Example 19 the title compound was prepared as a pale yellow powder (456 mg; yield, 88%) according to the method described in Example 9 1H-NMR (CD30D) 6: 1.51 9H), 4.88 2H), 4.76 2H), 6.97 J 8.60 Hz, 1H), 7.48 J 2.22 Hz, 1H), 7.52 (dd, J 2.22 Hz, 8.60 Hz, 1H).
SIMS 282 (M 1).
Example 4- 6, 7-tetrahydrothieno[ 3 ,2-c]pyridin-2yl)carbonyl]aminoacetyl]-2-benzyloxyphenoxyacetic acid 15 trifluoroacetate t-butyl 4-[[(5-t-butoxycarbonyl- 4 6 7 tetrahydrothieno[3,2-c]pyridin-2-yl)carbonyl]aminoacetyl]-2hydroxyphenoxyacetate Starting from 1.6 g (5.66 mmole) of the compound prepared in Referential Example 1 and 1.8g of compound prepared in Referential Example 28, the title compound was prepared as a yellow oily product (2.8 g; yield, 93%) according to the method described in Example 9 1H-NMR (CDC1 3 6: 1.50 9H), 1.51 9H), 2.88 (brs, 2H), 3.74 (brs, 2H), 4.50 (brs, 2H), 4.63 2H), 4.84 J 4.17 Hz, 2H), 6.91 J 8.60 Hz, 1H), 7.02 (brs, 1H), 7.33 1H), 7.54 (dd, J 2.22 Hz, 8.60 Hz, 1H), 7.62 J 2.22 Hz, 1H).
t-butyl 4-[[(5-t-butoxycarbonyl-4,5,6,7-tetrahydrothieno [3,2-c]pyridin-2-yl)carbonyl]aminoacetyl]-2-benzyloxy phenoxyacetate To the solution of the compound prepared in (100 mg, 0.183 mmole) in DMF (3 ml) were added potassium carbonate (30 mg) and benzyl bromide (50 pl), and the mixture was stirred at room temperature for 2 hours. The reaction mixture was diluted with ethyl acetate, washed with water and dried over anhydrous magnesium sulfate. After the inorganic salt was removed by filtration, the filtrate was concentrated under reduced pressure.
77 The oily product thus obtained was purified by column chromatography on silica gel with an eluent system of chloroform ethyl acetate 10 1 to give 54 mg of the title compound as a pale yellow oily product (yield, 46%).
1 H-NMR (CDC1 3 6: 1.49 9H), 1.50 9H), 2.87 (brs, 2H), 3.74 (brs, 2H), 4.50 2H), 4.69 2H), 4.81 J 4.10 Hz, 2H), 5.23 2H), 6.84 J 8.97 Hz, 1H), 7.00 (brs, 1H), 7.30- 7.63 8H).
FDMS 637 (M 1).
Starting from 54 mg of the compound prepared in the title compound was prepared as a pale yellow powder (30 mg; yield, 60%) according to the method described in Example 9 1H-NMR (CD 3 0D) 6: 3.29 (brs, 2H), 3.68 (brs, 2H), 4.40 (brs, 2H), 4.86 2H), 4.91 2H), 5.30 2H), 7.11 J 8.46 Hz, 15 1H), 7.40 7.62 6H), 7.74 J 2.05 Hz, 1H), 7.78 (dd, J 2.05 Hz, 8.46 Hz, 1H).
EDMS 481 1).
Referential Example 20 t-butyl[4-(aminoacetyl)-2-(ethoxycarbonylmethyloxy)] phenoxyacetate hydrochloride t-butyl [4-(azidoacetyl)-2-(ethoxycarbonylmethyloxy)] phenoxyacetate To the solution of the compound prepared in Referential Example 19 (500 mg, 1.63 mmole) in acetone (5 ml) were added potassium carbonate (270 mg) and ethyl bromoacetate (0.2 ml), and the mixture was stirred at room temperature for 6 hours. The reaction mixture was diluted with ethyl acetate, washed with water and dried over anhydrous magnesium sulfate. After the inorganic salt was removed by filtration, the filtrate was concentrated under reduced pressure. The oily product thus obtained was purified by column chromatography on silica gel with an eluent system of chloroform ethyl acetate 20 1 to give the title compound as a pale yellow crystal (552 mg, yield, 86%).
1H-NMR (CDC 3 6: 1.31 J 7.21 Hz, 3H), 1.48 9H), 4.27 J 7.21 Hz, 2H), 4.49 2H), 4.68 2H), 4.77 2H), 6.83 J 9.15 Hz, 1H), 7.49- 7.51 2H).
FDMS 393 Starting from 200 mg (0.508 mmole) of the compound prepared in the title compound was prepared as a pale yellow powder (183 mg; yield, 89%) according to the method described in Example 9 1H-NMR (CD 3 OD) 6: 1.38 J 7.21 Hz, 3H), 4.34 J 7.21 Hz, 2H), 4.62 2H), 4.89 2H), 4.93 2H), 7.14 J 8.60 Hz, 1H), 7.70 J 2.22 Hz, 1H), 7.80 (dd, J 2.22 Hz, 8.60 Hz, 1H).
SIMS 368 (M 1).
Example 51 4- 6,7 -tetrahydrothieno[3,2-c]pyridin- 2 15 yl)carbonyl]aminoacetyl]-2-(ethoxycarbonylmethyloxy)phenoxyacetic acid trifluoroacetate t-butyl [(5-t-butoxycarbonyl- 4 ,5,6,7tetrahydrothieno[3,2-c]pyridine-2-yl)-carbonyl]aminoacetyl]-2- (ethoxycarbonylmethyloxy)phenoxyacetate 20 Starting from 130 mg (0.448 mmole) of the compound prepared in Referential Example 1 and 180 mg of the compound prepared in Referential Example 25 the title compound was prepared as a pale yellow foam product (145 mg; yield, 51%) according to the method described in Example 9 'H-NMR (CDC1 3 6: 1.32 J 7.21 Hz, 3H), 2.86 (brs, 2H), 3.73 (brs, 2H), 4.28 J 7.21 Hz, 2H), 4.50 (brs, 2H), 4.70 (s, 2H), 4.78 2H), 4.84 J 4.16 Hz, 2H), 6.85 J 8.60 Hz, 1H), 7.32 1H), 7.55 J 1.94 Hz, 1H), 7.65 (dd, J 1.94 Hz, 8.60 Hz, 1H).
Starting from 140 mg (0.221 mmole) of the compound prepared in the title compound was prepared as a colorless solid product (78 mg; yield, 60%) according to the method described in Example 9 H-NMR (CD30D) 6: 1.38 J 7.21 Hz, 3H), 3.30 (brs, 2H), 3.67 (brs, 2H), 4.34 J 7.21 Hz, 2H), 4.41 2H), 4.89 2H), 4.94 2H), 7.15 J 8.60 Hz, 1H), 7.63 1H), 7.69 (d, J 2.22 Hz, 1H), 7.84 (dd, J 2.22 Hz, 8.60 Hz, 1H).
Example 52 4- 5,6 ,7-tetrahydrothieno[ 3 2-c]pyridin-2yl)carbonylamino]phenoxyacetic acid The title compound was prepared according to the method described in Example 9.
1H-NMR (DMSO-d 6 6: 3.10 2H), 3.47 2H), 4.25 2H), 4.64 2H), 6.90 J 8.8 Hz, 2H), 7.60 J 8.8 Hz, 2H).
Example 53 4- 4 ,5,6 ,7-tetrahydrothieno[3,2-c]pyridin-2yl)carbonyl]aminoacetyl]-2-hydroxyphenoxyacetic acid trifluoroacetate *15 Starting from the compound prepared in Example 50 (a) (100 mg, 0.183 mmole), the title compound (55 mg, yield 60%) was prepared as a pale orange solid product according to the method described in Example 9 1 H-NMR (CD30D) 6: 3.30 J 6.10 Hz, 2H), 3.67 J 6.10 Hz, 2H), 4.42 (brs, 2H), 4.90 J 5.00 Hz, 2H), 4.99 2H), 7.29 J 8.60 Hz, 1H), 7.66 1H), 7.89 J 1.94 Hz, 1H), 7.95 (dd, J 1.94 Hz, 8.60 Hz, IH).
Example 54 4- (4 6, 7-tetrahydrothieno[3,2-c]pyridin- 2 yl)carbonyl]aminoacetyl]-2-(N-ethyl-carbamoyloxy)phenoxyacetic acid trifluoroacetate t-butyl 4-[[(5-t-butoxycarbonyl- 4 ,5, 6 7 tetrahydrothieno[3,2-c]pyridin-2-yl)-carbonyl]aminoacetyl]-2-(Nethylcarbamoyloxy)phenoxyacetate To the solution of the compound prepared in Example (280 mg, 0.512 mmole) in DMF were added triethylamine (0.1 ml) and ethyl isocyanate (50 pl), and the mixture was stirred at room temperature for 4 hours. To the reaction solution was added water, and the mixture was acidified with 1N hydrochloric acid. The solution was extracted with ethyl acetate and dried Dver anhydrous magnesium sulfate. After the inorganic salt was removed by filtration, the filtrate was concentrated under reduced pressure. The oily product thus obtained was purified by column chromatography on silica gel with an eluent system of chloroform methanol 100 1 to give 175 mg of the title compound as a pale yellow foam product (yield, 1H-NMR (CDC1 3 6: 1.24 J 7.1 Hz, 3H), 1.48 9H), 1.49 9H), 2.88 (brs, 2H), 3.33 2H), 3.73 (brs, 2H), 4.50 (brs, 2H), 4.62 2H), 4.84 J 4.11 Hz, 2H), 5.27 (brs, 1H), 6.89 J 8.72 Hz, 1H), 7.04 (brs, 1H), 7.33 1H), 7.81 (s, 1H), 7.85 J 8.72 Hz, 1H).
FDMS 617 Starting from 50 mg (0.0809 mmole) of the compound prepared in the title compound was prepared as a colorless solid product (10 mg, yield, 21%) according to the method described in Example 9 H-NMR (CD30OD) 6: 1.28 J 7.21 Hz, 3H), 3.27 3.34 4H), 3.67 J 6.38 Hz, 2H), 4.41 (brs, 2H), 4.88 2H), 4.89 (s, 2H), 7.21.(d, J 8.88 Hz, 1H), 7.63 1H), 7.87 J 1.94 Hz, 1H), 8.02 (dd, J 1.94 Hz, 8.88 Hz, 1H).
Referential Example 26 2-aminoacetyl-4,5-di(t-butoxycarbonylmethyl)oxypyridine bishydrochloride 5-benzyloxy-2-hydroxymethyl-4-oxo-4H-pyran To the solution of kojic acid (28 g) in DMF (200 ml) was added potassium carbonate (32 and the mixture was stirred at room temperature for 30 minutes. After benzyl chloride (25 g) was added, the mixture was further stirred for 60 minutes. The solvent was evaporated. The residue was then extracted with ethyl acetate, washed with water and dried. After the solvent was evaporated, the residue was crystallized from ethyl acetate to give 5-benzyloxy-2-hydroxymethyl-4-oxo-4H-pyran (34.6 g).
1H-NMR (CD 3 0D) 6: 4.40 2H), 5.02 2H), 6.51 1H), 7.32 7.44 5H), 8.00 1H).
5-benzyloxy-4-hydroxy-2-hydroxymethylpyridine To the solution of the compound prepared in Referential 1 Example 26 (11 g) in methanol (20 ml) was added 25% aqueous ammonia (100 ml), and the mixture was stirred in a sealed tube at 1 00:C for 16 hours. The solvent was evaporated, and the residue was crystallized from warmed methanol to give benzyloxy-4-hydroxy-2-hydroxymethylpridine (10 g).
IH-NMR (CD 3 0D) 6: 4.51 2H), 5.09 2H), 6.43 1H), 7.29 7.45 6H).
5-benzyloxy-4-(t-butoxycarbonylmethyl)oxy-2hydroxymethylpyridine To the solution of the compound prepared in Referential Example 26 (5 g) in DMF (500 ml) were added potassium carbonate (3 g) and t-butyl bromoacetate (3.5 ml), and the mixture was stirred at room temperature for 4 hours. The reaction mixture was extracted with ethyl acetate, washed with water and 15 dried. After the solvent was evaporated, the residue was placed on a silica gel column and eluted with ethyl acetate n-hexane 1 1 to give 5-benzyloxy-4-(t-butoxycarbonylmethyl)oxy-2hydroxymethylpyridine (4.2 g).
1H-NMR (CDC13) 6: 1.47 9H), 4.61 2H), 4.65 2H), 5.17 2H), 6.75 1H), 7.30 7.45 5H), 8.06 1H).
S(d) 4-(t-butoxycarbonylmethyl)oxy-5-hydroxy-2hydroxymethylpyridine .To the solution of the compound prepared in Referential Example 26 (20 g) in methanol (200 ml) was added palladium-carbon (1 g) to carry out catalytic hydrogenation minutes). After the reaction mixture was filtered, the filtrate was concentrated to give 2-hydroxymethylpyridine (12.4 g).
1 H-NMR (CDC1 3 6: 1.50 9H), 4.58 2H), 4.63 2H), 6.75 1H), 8.10 1H).
4, 5- di-(t-butoxycarbonylmethyl)oxy- 2hydroxymethylpyridine To the solution of the compound prepared in Referential Example 26 (3 g) in DMF (30 ml) was added 60% NaH (0.47 g), and the mixture was stirred at room temperature for 1 hour. Then, reaction mixture was added dropwise the solution of t-butyl bromoacetate (1.73 ml) in DMF (30 ml) over a period of 5 hours.
The mixture was further stirred for 2 hours. After the solvent was evaporated, the residue was extracted with ethyl acetate, washed with water and dried, and the solvent was evaporated. The residue was placed on a silica gel column and eluted with ethyl acetate n-hexane 3 1 to give butoxycarbonylmethyloxy)-2-hydroxymethylpyridine (3.1 g).
1H-NMR (CDC1 3 6: 1.48 9H), 1.49 9H), 4.63 2H), 4.64 2H), 4.65 2H), 6.67 1H), 8.11 1H).
4,5-di(t-butoxycarbonylmethyl)oxy-2-formylpyridine To the solution of the compound prepared in Referential Example 26 (6 g) in dichloromethane (80 ml) was added manganese dioxide (10 and the mixture was stirred at room temperature for 16 hours.. The reaction mixture was filtered, and the filtrate was concentrated by evaporation. The residue was placed on a silica gel column and eluted with ethyl acetate nhexane 1 1 to give 4,5-di(t-butoxycarbonylmethyl)oxy- 2 formylpyridine (4.6 g).
1H-NMR (QDC1 3 6: 1.49 18H), 4.71 2H), 7.38 1H), 8.24 1H), 9.91 1H).
20 4, 5-di-(t-butoxycarbonylmethyloxy)- 2 hydroxy)ethylpyridine To the solution of the compound prepared in Referential Example :26 (4.6 g) in THF (50 ml) was added methyl magnesium bromide (1.02 mole/liter in THF, 17.9 ml) at -40'C, and the mixture was stirred for 30 minutes. Water was added to the reaction mixture. The resulting mixture was extracted with ethyl acetate, washed with water and dried. After the solvent was removed by evaporation, the residue was placed on a silica gel column and eluted with ethyl acetate n-hexane 2 1 to give 4,5-di(t-butoxycarbonylmethyloxy)-2-(1-hydroxy)ethylpyridine (2.82 g).
1H-NMR (CDC13) 6: 1.48 21H), 4.63 2H), 4.66 2H), 4.78 1H), 6.68 1H), 8.08 1H).
2-acetyl-4,5-di-(t-butoxycarbonylmethyloxy)pyridine To the solution of the compound prepared in Referential Example 26 (2.8 g) in dichloromethane (30 ml) was added manganese dioxide (6.3 and the mixture was stirred at room temperature for 16 hours. The reaction mixture was filtered, and the filtrate was concentrated to give 2-acetyl-4,5-di-(tbutoxycarbonylmethyloxy)pyridine (2.58 g).
1H-NMR (CDC1 3 6: 1.49 18H), 2.66 3H), 4.70 2H), 4.74 2H), 7.50 1H), 8.14 1H).
2-bromoacetyl-4,5-di(t-butoxycarbonylmethyl)oxypyridine To the solution of the compound prepared in Referential Example 26 (1.0 g) in dichloroethane (10 ml) were added triethylamine (0.9 ml) and trimethylsilyltrifluoromethane sulfonate (0.54 ml), and the mixture was stirred at -40:C for minutes. After N-bromosuccinimide (0.49 g) was added, the mixture was stirred at -35fC for 30 minutes- The reaction mixture was concentrated, extracted with ether, washed with aqueous sodium hydrogen carbonate and water and dried. The solvent was removed 15 by evaporation. The residue was placed on a silica gel column and eluted with ethyl acetate n-hexane 1 3 to give 2bromoacetyl-4,5-di-(t-butoxycarbonylmethyloxy)pyridine (561 mg).
1H-NMR (CDC13) 6: 1.49 18H), 4.70 2H), 4.75 2H), 4.81 2H), 7.52 1H), 8.13 1H).
20 2-azidoacetyl- 4 To the solution of the compound prepared in Referential Example 26 (550 mg) in DMF (15 ml) was added sodium azide mg), and the mixture was stirred at room temperature for minutes. The reaction mixture was concentrated. The residue was extracted with ethyl acetate, washed with water, dried, and the solvent was removed by evaporation. The residue was placed on a silica gel column and eluted with ethyl acetate n-hexane 1 3 to give 2-azidoacetyl-4,5-di-(t-butoxycarbonylmethyloxy) pyridine (442 mg).
1H-NMR (CDC1 3 6: 1.49 9H), 1.50 9H), 4.70 2H), 4.74 2H), 4.80 2H), 7.50 1H), 8.10 1H).
2-aminoacetyl-4,5-di(t-butoxycarbonylmethyloxy)pyridine bishydrochloride To the solution of the compound prepared in Referential Example 26 (100 mg) in methanol (8 ml) CHCl 3 (2 ml) were added 10% palladium-carbon (10 mg) and lN hydrochloric acid ml), and catalytic hydrogenation was conducted for 3 hours. The reaction mixture was filtered. After the filtrate was concentrated, water was added to the concentrate. The mixture was lyophilized to give 2-aminoacetyl- 4 butoxycarbonylmethyloxy)pyridine bishydrochloride (104 mg).
1 H-NMR (CD 3 0D) 6: 1.49 9H), 1.49 9H), 7.63 1H), 8.27 1H).
Example 2- 5,6,7-tetrahydrothieno[ 3 2-c]pyridin-2acid bistrichloroacetate To the solution of the compound prepared in Referential Example 40 (42 mg) in DMF (1 ml) were added BOP (66 mg) and N- 15 methylmorpholine (0.05 ml), and the mixture was stirred at room temperature for 1 hour. Then, 5-t-butoxycarbonyl- 4 ,5,6, 7 tetrahydrothieno[3,2-c]pyridin-2-ylcarboxylic acid (70 mg) was added. The.mixture was further stirred for 4 hours. The reaction mixture was extracted with ethyl acetate, washed with water, dried. After the solvent was removed by evaporation, the residue was placed on a silica gel column and eluted with ethyl acetate n-hexane 1 2 to give di-t-butyl-2-[2-[(5-t-butoxycarbonyl- 4, 5, 6 7-tetrahydrothieno[3, 2-c]pyri din 2 yl)carbonyljaminoacetyl]pyridinyl- 4 ,5-dioxydiacetate (46 mg).
H-NMR (CDCi 3 6: 1.49 9H), 1.50 9H), 2.87 (brs, 2H), 3.73 (brs, 2H), 4.50 2H), 4.71 2H), 4.76 2H), 5.07 2H), 6.82 (brs, 1H), 7.31 1H), 7.50 1H), 8.15 (s, 1H).
To the solution of the compound prepared in (44 mg) in anisole (0.06 ml) was added trifluoroacetic acid (0.3 ml), and the mixture was stirred at room temperature for 30 minutes. After diisopropyl ether was added to the mixture, crystals deposited were collected by filtration and washed with diethyl ether to give the title compound (30 mg).
1H-NMR (DMSO-d 6 6: 3.04 2H), 4.19 2H), 4.82 4.93 (m, 6H), 7.41 1H), 7.59 1H), 8.23 1H).
Example 56 Di-n-butyl [[4-[[[(4,5,6,7-tetrahydrothieno[3,2-c]pyridin- 2 yl)carbonyl]amino]acetyl]-o-phenylene]dioxy]diacetate trifluoroacetate To the solution of the compound prepared in Referential Example 1 (392 mg, 1.64 mmole) in DMF (10 ml) were added BOP (800 mg, 1.81 mmole), N-methylmorpholine (0.40 ml, 3.62 mmole) and din-butyl [[4-(aminoacetyl)-o-phenylene]dioxy]diacetate (710 mg, 164 mmole), and the mixture was stirred at room temperature for 1.5 hours. The reaction mixture was poured onto ice-water, extracted with ethyl acetate and then washed with water. After the ethyl acetate layer was dried, over anhydrous magnesium sulfate, the ethyl acetate was removed by evaporation. The residue was purified by column chromatography on silica gel with 15 an eluent system of hexane ethyl acetate 3 1 1 1 to give 500 mg of di-n-butyl [[4-[[2-[(5-t-butoxycarbonyl- 4 ,5, 6 7 tetrahydrothieno[3,2-c]pyridin-2-yl)carbonyl]amino]acetyl]-ophenylene]dioxy]diacetate (yield, 1 H-NMR (CDC1 3 6: 0.92 J 7 Hz, 3H), 0.92 J 7 Hz, 3H), 20 1.36 4H), 1.50 9H), 1.63 4H), 2.87 2H), 3.74 (s, 2H), 4.22 J 7 Hz, 2H), 4.22 J 7 Hz, 2H), 4.50 (s, 2H), 4.79 2H), 4.81 2H), 4.84 J 4 Hz, 2H), 6.89 (d, J 8 Hz, 1H), 7.00 1H), 7.33 1H), 7.55 J 2 Hz, 7.65 (dd, J=2.8Hz, 1H).
EIMS 660 To the compound prepared in (470 mg, 0.711 mmole) were added anisole (0.7 ml) and trifluoroacetic acid (2.4 ml), and the mixture was stirred at room temperature for 1 hour. After the solvent was removed by evaporation, the residue was extracted with water and washed with ether. The aqueous layer was combined and lyophilized to give 344 mg of the title compound as a colorless crystal (yield, 72%).
1H-NMR (CD 3 D) 6: 0.92 J 7 Hz, 3H), 0.93 J 7 Hz, 3H), 1.37 4H), 1.63 4H), 3.20 J 6 Hz, 2H), 3.58 J 6 Hz, 2H), 4.20 J 6 Hz, 2H), 4.21 J 6 Hz, 2H), 4.32 2H), 4.79 2H), 4.83 2H), 4.89 2H), 7.05 J 8 Hz, 1H), 7.54 1H), 7.60 J 2 Hz, 1H), 7.74 (dd, J 86 2, 8 Hz, 1H).
EIMS 560 Example 57 Dicyclohexyl [[4-[[[(4,5,6,7-tetrahydrothieno[3,2-c]pyridin- 2 yl)carbonyl]amino]acetyl]-o-phenylene]dioxy]diacetate Free derivative of the compound prepared in Example 34 (700 mg, 1.39 mmole) was stirred in the mixture of cyclohexanol (14 ml) and chloroform (7 ml) saturated with hydrochloric acid at room temperature overnight. After chloroform was added to the reaction mixture, the resulting mixture was neutralized with a sodium hydrogen carbonate aqueous. The chloroform layer was separated and dried with anhydrous magnesium sulfate. The solvent was removed by evaporation. Purification by column chromatography 15 on silica gel (CHC1 3 MeOH 10 1 4 1) gave the title compound (531 mg, yield, 57%).
1IH-NMR (CDC1 3 6: 1.20 1.95 20H), 2.84 2H), 3.17 (t, J 5 Hz, 3.93 2H), 4.76 2H), 4.79 2H), 4.84 (d, J 4 Hz, 2H), 4,90 2H), 6.88 J 8 Hz, 1H), 6.98 (s, 20 1H), 7.29 1H), 7.54 J 2 Hz, 1H), 7.63 (dd, J 2, 8 Hz, 1H).
EIMS 612 Referential Example 27 4-Cyanocyclohexylcarboxylic acid To the solution of methyl 4-cyanocyclohexylcarboxylate (470 mg, 2.81 mmole) prepared according to the method described in J. Am. Chem. Soc., 82, 2547 (1960) in methanol (5 ml) was added IN sodium hydroxide (2.8 ml) under ice-cooling, and the mixture was stirred overnight during which the temperature of the mixture was left rising up to room temperature. After the reaction mixture was concentrated, water was added. The resulting mixture was washed with ether and acidified with 5N hydrochloric acid. After extracted thrice with chloroform, the mixture was dried with anhydrous magnesium sulfate. After the inorganic salt was removed by filtration, the filtrate was concentrated under reduced pressure to give 362 mg of the title compound as a colorless crystal (yield, 84%).
1 H-NMR (CDC1 3 6: 1.50 1.70 4H), 2.07 2.18 4H), 2.38 2.54 2H).
Example 58 [[trans-4-amidinocyclohexyl]carbonyl-N-methylamino]acetyl]o-phenylene]dioxy]diacetic acid trifluoroacetate Di-t-butyl [[4-[[[trans-4-cyanocyclohexyl]carbonyl-Nmethylamino]acetyl]-o-phenylene]dioxy]diacetate Starting from the compound prepared in Referential Example 1 (153 mg, 1 mmole) and 90 (410 mg), the title compound (325 mg, yield, 65%) was obtained- according to the method described in Example 9 IH-NMR (CDC1 3 6: 1.47 9H), 1.48 9H), 1.58 1.68 (m, 15 4H), 1.90 2.00 2H), 2.20 2.26 2H), 2.45 2.55 (m, 1H), 2.65 2.72 1H), 3.11 3H), 4.63 2H), 4.67 (s, 4.74 2H), 6.80 J 8.33 Hz, 1H), 7.46 J 1.94 *Hz, 1H), 7.55 (dd, J 1.94 Hz, 8.33 Hz, 1H).
EIMS 544 20 [[4-[[[trans-4-thiocarbamoylcyclohexyl]carbonyl-Nmethylamino]acetyl]-o-phenylene]dioxy]diacetic acid Into the solution of the compound prepared in (655 I&o"e mg, 1.2 mmole) in the mixture of pyridine (15 ml) and triethylamine (1 ml) was streamed a hydrogen sulfide gas for 1 hour, and the mixture was stirred in a sealed tube at 50'C for ^hours. After the reaction mixture was cooled to room temperature, it was diluted with ethyl acetate, and washed twice with each of a 5% aqueous sodium hydrogen carbonate and a IM potassium hydrogen sulfate solution. After the organic layer was dried over anhydrous magnesium sulfate, the inorganic salt was removed by filtration. The filtrate was concentrated under reduced pressure, and the oily product thus obtained was purified by column chromatography on silica gel with an eluent system of chloroform methanol 50 1 to give 71 mg of the thioamide derivative as a yellow oily product (yield, 1H-NMR (CDC13) 6: 1.48 9H), 1.49 9H), 1.58 1.65 (m, 4H), 1.72 1.82 2H), 1.92 2.00 2.54 2.61 (m, 1H), 2.69 2.78 1H), 3.13 3H), 4.64 2H), 4.68 (s, 2H), 4.75 2H), 6.83 J 8.46 Hz, 1H), 7.47 J 1.79 Hz, 1H), 7.58 (dd, J 1.79 Hz, 8.46 Hz, 1H).
SIMS 579 (M 1).
[trans-4-( imino( methylthio)methyl cyclohexyl]carbonyl-N-methylamino]acetyl]-o-phenylene] dioxy]diacetic acid To the solution of the compound prepared in (70 mg, 0.121 mmole) in acetone (7 ml) was added methyl iodide (0.7 ml), and the mixture was heated at reflux temperature for 1 hour.
After the reaction mixture was concentrated under reduced pressure, the oily product thus obtained was purified by column 15 chromatography on silica gel with an eluent system of chloroform methanol 100 1 to give 69 mg of the iminothiomethyl derivative as a yellow oily product (yield, 97%).
1 H-NMR (CDC 3 6: 1.47 9H), 1.48 9H), 1.55 1.69 (m, 4H), 1.92 1.98 2H), 2.02 2.09 2H), 2.25 3H), 2.30 20 2.43 1H), 2.60 2.68 1H), 3.11 3H), 4.63 2H), *4.67 2H), 4.74 2H), 6.80 J 8.32 Hz, 1H), 7.47 (d, J 1.94 Hz, 1H), 7.57 (dd, J 1.94 Hz, 8.32 Hz, 1H).
SIMS 593 (M 1).
Di-t-butyl [[4-[[[trans-4-amidinocyclohexyl]carbonyl-Nmethylamino]acetyl]-o-phenylene]dioxy]diacetate To the solution of the compound prepared in (62 mg, 0.107 mmole) in methanol (3 ml) was added ammonium acetate, and the mixture was stirred at reflux temperature for 1.5 hours. The reaction mixture was concentrated under reduced pressure to give 56 mg of the amidine derivative as a pale yellow oily product (yield, 93%).
IH-NMR (CD 3 0OD) 6: 1.48 9H), 1.49 9H), 1.53 1.72 (m, 4H), 1.90 1.98 2H), 2.00 2.06 2H), 2.45 2.53 (m, 1H), 2.82 2.88 1H), 2.94, 3.18 (2s, 3H), 4.69, 4,71 (s, 2H), 4.76, 4.78 (2s, 2H), 4.83, 4.91 (2s, 2H), 6.99, 7.10 (2d, J 8.46 Hz, 1H), 7.54, 7.58 (2d, J 2.05 Hz, 1H), 7.68, 7.70 (2dd, J 2.05 Hz, 8.46 Hz, 1H).
To the solution of the compound prepared in (50 mg, 0.089 mmole) in anisole was added trifluoroacetic acid under icecooling. The mixture was stirred for 5 hours, during which the temperature of the mixture was left rising up to room temperature. After isopropyl ether was added to the reaction mixture, solids deposited were collected to give 31 mg of the title compound as the colorless solid (yield, 62%).
1 H-NMR (CDO3D) 6: 1.48 1.72 4H), 1.87 2.03 4H), 2.45 2.55 1H), 2.80 2.88 1H), 2.95, 3.18 (2s, 3H), 4.77, 4.80 (2s, 2H), 4.83 2H), 4.84 2H), 7.04, 7.08 (2d, J 8.72 Hz, 1H), 7.57, 7.62 (2d, J 1.80 Hz, 1H), 7.68, 7.74 (2dd, J 1.80 Hz, 8.72 Hz, 1H).
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S
-TO N' 00 S 0 0. S I t t Table 1 (cont. Example A- X2$ 4a NJ -CONHCHI Coo2i CH- -0c CI 2 CO0OL1I 0 C 1-I 2 CO0 l]- 0 -C112 C112 C113 -0 C1 2 Ph _~OCH 2 COQEt t t CO0N Ht C 0N 11C H 2 GO0- 0H CH- -OcI-1 2 COO-11-BI t -OCHI 2 Co0o-J' -0-C 0- NII -E 0OC 11 2 coo r-1 0OC 1-1 2 C00-r1i-B u 0C c112Co00 II N 58 N -CO0N Cl1-I 2 CO0 Cl 3 0 CF1 2 CO0OII. -O0C 11 2 COOJI1- Pharmacological test 1 Inhibition of platelet aggregation The effect of the compound according to the present invention on inhibiting the platelet aggregation was examined with human PRP (platelet rich plasma).
Nine volumes of a blood sample was taken out of the vein of a normal male human with a syringe in which one volume of a 3.8% sodium citrate solution was charged. The blood sample was centrifuged at 170 x g at room temperature for 10 minutes. The supernatant thus obtained was isolated as PRP. The residual blood sample that PRP had been taken out was centrifuged at 2,700 x g for 15 minutes. The supernatant was then taken as platelet poor plasma (PPP).
Platelet aggregation test was conducted with an aggligometer (PAM-8C; manufactured by MEBANICKS Co., Ltd.).
Compounds to be tested were dissolved in a 50% DMSO saline, a methanol saline or physiological saline. The compound and PRP were preincubated for 2 minutes. The ADP (CHRONO-PAR REAGENTS 384 ADP, CHRONO-LOG Corp.) was used as an inducer in the form of a dilution with saline so that the final concentration is 5 pM.
20 Anti-platelet aggregation activity was obtained as an inhibition rate to platelet aggregation effect of ADP in the absence of a compound to be tested as follows:
S
Anti-platelet aggregation activity 1 Physiological test 2 Inh platelet GPIIb/IIIa Inhibiting effect present invention on the fibrinogen was examined w GPIIb/IIIa bonding assay u ligand.
Aggregation rate by ADP with test compound Aggregation rate by ADP without test compound x 100 Libition of fibrinogen bonding to of the compound according to the binding of platelet GPIIb/IIIa and ith a solid phase human platelet ising biotinylated fibrinogen as a Human platelet GPIIb/IIIa was purified according to the method described by Pytela, R. et al. (Science, 231, 1559-1561 (1986)).
Briefly, platelets collected from healthy adults was washed with TBS (0.15M NaCl/50 mM Tris-HCI buffer, pH containing glucose. The same amount of TBS containing 50 mM octylglucoside and 2 mM PMSF was added to the pellet obtained in order to solubilize the membrane protein at 4'C for minutes. The extract was centrifuged at 3,500 x g at 4:C for minutes, 1 mM CaCl- and 1 mM MgCl were added to the supernatant to give a sample for affinity chromatography. The solubilized protein solution was adsorbed on a GRGDSPK-Sepharose column which had been equilibrated with TBS (Buffer A) containing mM Octylglucoside, 1 mM CaCl, and -MgCl2. After the column was washed with Buffer A, the adsorbed GPIIb/IIIa was eluted with Buffer A containing 2 mM GRGDSP peptide.
15 GRGDSPK-Sepharose was prepared by coupling GRGDSPK peptide and CNBr-activated Sepharose 4B (Pharmacia) according to the instruction of the manufacturer.
Then, solid phase human platelet GPIIb/IIIa bonding assay was carried out according to the method of Mori et al. (NIPPON 20 KESSEN SHIKETSU GAKKAISHI, 2 323-329 (1991)).
Purified human platelet GPIIb/IIIa was adjusted in an amount of 2 pg/ml and adsorbed in an amount of 50 pl on a 96 well-microtiter plate at 4'C overnight. After washing with TBS containing 1 mM CaCl 2 and 1 mM MgCl, 100 pl of 1% bovine serum 25 albumin was added, blocking was conducted at 41C overnight. After washing with TBS containing 0.01% Tween 20 (TBS-Tween reaction was conducted at room temperature for 4 hours by adding pl of biotinylated fibrinogen of which the concentration had been adjusted to 1 pg/ml and 50 pl of the test compound which had been adjusted to appropriate concentrations. After washing with TBS-Tween 20, 50 pl of peroxidase labelled avidin which had been diluted with TBS to 4,000 times was added. The reaction was continued for 20 minutes. After washing with TBS-Tween reaction was conducted for 5 minutes by adding 50 ul of a solution of a 10 time diluted peroxidase substrate buffer in which 1 ml/ml of ABTS (2,2'-Azino-bis(3-ethylbenzthiazoline- 6 sulfonic acid) was dissolved. A 50 pl portion of a 0.1 M citrate buffer containing 0.05% NaN 3 was added to stop the reaction, and absorbance at 415 nm was measured.
The rate of Bonding inhibition was calculated from the following formula: Binding Inhibition 1 Rate Absorbance with test compound Absorbance without test compound x 100 Anti-platelet aggregation activities and effects of the compound according to the present invention on bonding inhibition between platelet GPIIb/IIIa and fibrinogen are shown in the following tables.
t20 25 /3 2U Compound of Example Table 2 Pharmacological Test 1 68%*1 85%*1 48%*1 79%*1 85%*1 Pharmacological Test 2 51%* 2 73%*2 27%*- 73%* 3 82%* 3 in inhibition rate at 10 6
M
inhibition rate at 10 M inhibition rate at 10-7M 100 30 Compound of Example 18 19 21 22 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 47 56 57 58 Table 3 Pharmacological Test 1 (IC 50, PM) 0.14 0.55 0.5 1.2 2.5 >10 0. 23 1.9 0.15 0.48 1.7 0.93 0.35 9.0 4.9 8.0 0.26 4.0 3.3 0.34 2.8 0.72 0.46 6.4 1.1 3.2 0. 14 Pharmacological Test 2
(IC
50
PM)
1.7 3.6 34 27 1.4 8.7 14 26 42 >100 >100 >100 2.7 100 100 2.2 5.7 26 101 P:\OPER\RMH6263&94.SPE 14/1/98 Throughout this specification and the claims which follow, unless the context requires otherwise, the word "comprise", or variations such as "comprises" or "comprising", will be understood to imply the inclusion of a stated integer or group of integers but not the exclusion of any other integer or group of integers.
S102 102

Claims (18)

1. A compound represented by the formula or a pharmaceutically acceptable salt and solvate thereof: A-B-Y R 1 X R 2 wherein R 1 represents a group -W-(CH- i -COOR 3 where W represents O- or a bond, R 3 represents hydrogen, lower alkyl, C 5 -7 cycloalkyl or an ester moiety which may be removed under a physiological condition, and i is an integer from 1 to 4, R represents hydrogen or a group -W-(CH 2 )i-COOR where W, R 3 and i have the same meanings as defined above, or a group OR 4 where R 4 represents hydrogen, lower alkyl, mono lower alkylaminocarbonyl or phenyl-lower alkyl, X represents CH or N, Y represents a group -(CO)k-N(R where k represents 0 or 1, R 5 represents hydrogen; lower alkyl in which one or more hydrogen atoms may be substituted by hydroxyl, halogen, amino, carboxyl, lower alkoxy, lower alkylamino, or lower alkoxycarbonyl; phenyl-lower alkyl group in which one or more hydrogen atoms in the phenyl moiety may be substituted by hydroxyl, halogen, amino, carboxyl, lower alkoxy, lower alkylamino, lower alkoxycarbonyl or halo-lower alkyl; or acyl, Z represents a bond, or a group -(CH2) -CO- or a group (CH 2 )m-CHR6-, where m is an integer from 1 to 3 and R 6 represents hydrogen or hydroxyl, (ii) a group -CO-(CH2)m-N(R where k, m and R 5 have the same meanings as defined above, 103 P'\OPER\PDB52084-9a.spc.doc-19A6/00 -104- or (iii) a group -(CO)k-Het where Het represents a five- or six-membered heterocyclic moiety containing 1 to 4 nitrogen atoms, which may optionally contain an oxygen atom or a sulfur atom when the moiety contains 1 or 2 nitrogen atoms, and k is 0 or 1. A represents the following group (II): R 7 RS/ wherein D represents a group -(CH 2 where s is an integer from 1 to 4, or a group and R 7 and R 8 represent independently hydrogen, lower alkyl, acyl, aromatic acyl group which may be substituted, or amidino, B represents a bond, C1-6 alkylene or C2-6 alkenylene
2. A compound according to claim 1, wherein Y represents the group where k is 1, or the group (CO)k-Het-.
3. A compound according to claim 2, wherein B represents a bond.
4. A compound according to claim 2, wherein X represents CH. P:\OPER\PDB\52084-98.pec.doc-19/06/OO -105- A compound according to claim 2, wherein Z represents the group -(CH 2 )m-CO- or the group -(CH 2 )m-CHR 6 where R 6 represents hydrogen.
6. A compound according to claim 2, wherein D represents the group and both of R 7 and R 8 represent hydrogen.
7. A compound according to claim 1, wherein Y represents the group -(CO)k-N(R 5 wherein k is 1, or the group -NHCO-, or the group -(CO)k-Het-, and B represents a bond or C 1 -6 alkylene.
8. A compound according to claim 7, wherein Z represents the group -(CH 2 )m-CO- or the group -(CH 2 )m-CHR 6 where R 6 represents hydrogen, and B represents a bond or C1- 6 alkylene.
9. A compound according to claim 2, wherein Y represents the group -(CO)k-Het-. [[4-[[[(5,6,7,8-tetrahydro-4H-thieno[2,3-d]azepin-2- yl]carbonyl]amino]acetyl]-o-phenylene]dioxy]diacetic acid trifluoroacetate.
11. [[4-[[[(trans-4-amidinocyclohexyl]carbonyl-N- methylamino]acetyl]-o-phenylene]dioxy]diacetic acid trifluoroacetate.
12. A pharmaceutical composition comprising a compound according to claim 1 or a pharmacologically acceptable P:\OPER\PDB52084-98.1pec.doc-19/06/00 -106- salt or solvate thereof together with a pharmacologically acceptable carrier.
13. Use of a compound according to claim 1 in the manufacture of a medicament for platelet aggregation inhibition.
14. Use of a compound according to claim 1 in the manufacture of a medicament for the treatment or prophylaxis of thrombotic diseases. Use according to claim 14, wherein said thrombotic diseases are cerebral infarction, cardiac infarction, angina pectoris or peripheral arterial atresia.
16. A method for inhibiting platelet aggregation in a mammal comprising the step of administering to said mammal an inhibiting effective amount of a compound according to claim 1.
17. A method for the treatment or prophylaxis of thrombotic diseases comprising the step of administering to a mammal a therapeutically effective amount of a compound according to claim 1.
18. A method according to claim 17, wherein the thrombotic diseases are cerebral infarction, cardiac infarction, angina pectoris or peripheral arterial atresia.
19. A compound according to claim 1 substantially as hereinbefore described. P:\OPERPDBI52084-98.spec.doc-19/06/0 -107- A composition according to claim 12 substantially as hereinbefore described.
21. A use according to claim 13 or 14 substantially as hereinbefore described.
22. A method according to claim 16 or 17 substantially as hereinbefore described. dated this 19 h day of June 2000 Meiji Seika Kabushiki Kaisha By its Patent Attorneys DAVIES COLLISON CAVE
AU52084/98A 1993-03-17 1998-01-15 Novel compound with platelet aggregation inhibitor activity Ceased AU723180B2 (en)

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