AU637354B2 - Genetic units for inhibiting the function of rna - Google Patents
Genetic units for inhibiting the function of rnaInfo
- Publication number
- AU637354B2 AU637354B2 AU51301/90A AU5130190A AU637354B2 AU 637354 B2 AU637354 B2 AU 637354B2 AU 51301/90 A AU51301/90 A AU 51301/90A AU 5130190 A AU5130190 A AU 5130190A AU 637354 B2 AU637354 B2 AU 637354B2
- Authority
- AU
- Australia
- Prior art keywords
- rna
- units
- inhibiting
- genetic
- unit
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/11—Antisense
- C12N2310/111—Antisense spanning the whole gene, or a large part of it
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
- C12N2310/121—Hammerhead
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Enzymes And Modification Thereof (AREA)
- Nitrogen Condensed Heterocyclic Rings (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention relates to a genetic unit, which is optionally in multicopy form, for the inhibition of RNA. The unit contains the transcription units necessary for transcription by polymerase III, and a DNA which codes for inhibiting RNA and which is arranged within the unit in such a way that the transcribed RNA is part of the polymerase III transcript. These units can be used to achieve increased stability of the inhibiting RNA, which can be in the form of ribozymes or antisense RNAs, while there is no reduction in the activity. The invention furthermore relates to a process for introducing the genetic units into the cell, to the use of these units and to pharmaceutical formulations containing them.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AT60989 | 1989-03-16 | ||
AT609/89 | 1989-03-16 |
Publications (2)
Publication Number | Publication Date |
---|---|
AU5130190A AU5130190A (en) | 1990-11-01 |
AU637354B2 true AU637354B2 (en) | 1993-05-27 |
Family
ID=3495118
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AU51301/90A Expired AU637354B2 (en) | 1989-03-16 | 1990-03-15 | Genetic units for inhibiting the function of rna |
Country Status (20)
Country | Link |
---|---|
EP (1) | EP0387775B1 (en) |
JP (1) | JP3330930B2 (en) |
KR (1) | KR900014591A (en) |
AT (1) | ATE140961T1 (en) |
AU (1) | AU637354B2 (en) |
CA (1) | CA2012312C (en) |
DD (1) | DD297838A5 (en) |
DE (1) | DE59010432D1 (en) |
DK (1) | DK0387775T3 (en) |
ES (1) | ES2090049T3 (en) |
FI (1) | FI105103B (en) |
GR (1) | GR3020935T3 (en) |
HU (2) | HU215187B (en) |
IE (1) | IE74850B1 (en) |
IL (1) | IL93754A (en) |
NO (1) | NO306623B1 (en) |
NZ (1) | NZ232917A (en) |
PT (1) | PT93440B (en) |
RU (1) | RU2136751C1 (en) |
ZA (1) | ZA901973B (en) |
Families Citing this family (32)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH04505261A (en) * | 1989-05-10 | 1992-09-17 | スローン―ケツテリング・インステイテユート・フオー・キヤンサー・リサーチ | Stably transformed eukaryotic cells containing transcribable foreign DNA under the control of a Pol III promoter |
DE4091533T (en) * | 1989-08-31 | 1992-01-30 | ||
JP2507895B2 (en) * | 1989-12-19 | 1996-06-19 | 工業技術院長 | New synthesis system of ribozyme |
CA2084790C (en) * | 1990-06-19 | 2002-04-09 | Philip Anthony Jennings | Endonucleases |
US6008343A (en) * | 1990-06-19 | 1999-12-28 | Gene Shears Pty. Ltd. | Nucleotide based endonucleases |
DE552178T1 (en) | 1990-10-12 | 1994-02-03 | Max Planck Gesellschaft | MODIFIED RIBOZYMS. |
DE4104186A1 (en) * | 1991-02-12 | 1992-08-13 | Genentech Inc | NEW COMPLEXES INCLUDING ENDOCYTOSIS IN HIGHER EUKARYOTIC CELLS, NUCLEIC ACID |
FR2687411A1 (en) * | 1992-02-13 | 1993-08-20 | Nice Sophia Antipolis Universi | Vector comprising a viral gene transcribed by RNA polymerase III, and process for the intercellular production of RNA |
AU4259193A (en) * | 1992-04-28 | 1993-11-29 | Frank Andreas Harald Meyer | Medicament for the gene-therapeutic treatment of human beings, animals and plants, especially to block virus multiplication and carcinogenes and process for producing the medicament |
CA2135499A1 (en) * | 1992-05-14 | 1993-11-25 | James D. Thompson | Method and reagent for inhibiting cancer development |
US5989906A (en) * | 1992-05-14 | 1999-11-23 | Ribozyme Pharmaceuticals, Inc. | Method and reagent for inhibiting P-glycoprotein (mdr-1-gene) |
US5610052A (en) * | 1992-08-26 | 1997-03-11 | Ribozyme Pharmaceuticals Inc. | Enzymatic RNA with activity to ras |
US5827935A (en) * | 1992-05-27 | 1998-10-27 | City Of Hope | Chimeric tRNAlys -ribozyme molecules |
EP0596901B1 (en) * | 1992-05-27 | 2000-08-09 | City Of Hope | CHIMERIC tRNA LYS -RIBOZYME MOLECULES |
JPH08500971A (en) * | 1992-06-29 | 1996-02-06 | ジーン・シアーズ・ピーティーワイ・リミテッド | Nucleic acids and their use for controlling viral pathogens |
WO1994013798A1 (en) * | 1992-12-16 | 1994-06-23 | Bergmann Johanna E | Agents for the prevention and treatment of human alzheimer's disease |
DE69333216T2 (en) * | 1993-07-06 | 2004-06-24 | Université de Nice-Sophia Antipolis | Vector, contains a virus gene transcribed in ARN polymerase III |
US5837503A (en) * | 1993-07-07 | 1998-11-17 | Universite De Nice-Sophia-Antipolis | Vector containing viral gene transcribed by RNA polymerase III and methods for use |
US5817635A (en) * | 1993-08-09 | 1998-10-06 | Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. | Modified ribozymes |
AU692208B2 (en) * | 1994-01-24 | 1998-06-04 | City Of Hope | Inhibitors and target molecule co-localization |
DE4424761C1 (en) * | 1994-07-04 | 1995-06-08 | Max Planck Gesellschaft | Expression cassette for continuous, stable prodn. of ribozyme |
EP0837933A4 (en) | 1995-06-07 | 2003-05-21 | Commw Scient Ind Res Org | Optimized minizymes and miniribozymes and uses thereof |
FR2755976B1 (en) | 1996-11-15 | 1999-01-15 | Idm Immuno Designed Molecules | NOVEL COMPLEXES OF NUCLEIC ACIDS AND POLYMER SUBSTITUTED BY RESIDUES CAUSING THE DESTABILIZATION OF CELL MEMBRANES |
US6057156A (en) | 1997-01-31 | 2000-05-02 | Robozyme Pharmaceuticals, Inc. | Enzymatic nucleic acid treatment of diseases or conditions related to levels of epidermal growth factor receptors |
WO1999020753A2 (en) * | 1997-10-21 | 1999-04-29 | Ribozyme Pharmaceuticals, Inc. | Peptide bond formation using nucleic acid catalysts |
US6506559B1 (en) | 1997-12-23 | 2003-01-14 | Carnegie Institute Of Washington | Genetic inhibition by double-stranded RNA |
EP1555317B1 (en) | 1998-03-20 | 2011-09-28 | Commonwealth Scientific And Industrial Research Organisation | Synthetic genes and genetic constructs comprising the same |
AUPP249298A0 (en) | 1998-03-20 | 1998-04-23 | Ag-Gene Australia Limited | Synthetic genes and genetic constructs comprising same I |
CA2361201A1 (en) | 1999-01-28 | 2000-08-03 | Medical College Of Georgia Research Institute, Inc. | Composition and method for in vivo and in vitro attenuation of gene expression using double stranded rna |
US6423885B1 (en) | 1999-08-13 | 2002-07-23 | Commonwealth Scientific And Industrial Research Organization (Csiro) | Methods for obtaining modified phenotypes in plant cells |
US20020132257A1 (en) | 2001-01-31 | 2002-09-19 | Tony Giordano | Use of post-transcriptional gene silencing for identifying nucleic acid sequences that modulate the function of a cell |
AUPR621501A0 (en) | 2001-07-06 | 2001-08-02 | Commonwealth Scientific And Industrial Research Organisation | Delivery of ds rna |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4987071A (en) * | 1986-12-03 | 1991-01-22 | University Patents, Inc. | RNA ribozyme polymerases, dephosphorylases, restriction endoribonucleases and methods |
-
1990
- 1990-03-13 EP EP90104701A patent/EP0387775B1/en not_active Expired - Lifetime
- 1990-03-13 DK DK90104701.9T patent/DK0387775T3/en active
- 1990-03-13 ES ES90104701T patent/ES2090049T3/en not_active Expired - Lifetime
- 1990-03-13 DE DE59010432T patent/DE59010432D1/en not_active Expired - Lifetime
- 1990-03-13 AT AT90104701T patent/ATE140961T1/en not_active IP Right Cessation
- 1990-03-14 HU HU901584A patent/HU215187B/en unknown
- 1990-03-14 NZ NZ232917A patent/NZ232917A/en unknown
- 1990-03-14 DD DD90338709A patent/DD297838A5/en not_active IP Right Cessation
- 1990-03-15 PT PT93440A patent/PT93440B/en not_active IP Right Cessation
- 1990-03-15 NO NO901213A patent/NO306623B1/en not_active IP Right Cessation
- 1990-03-15 JP JP06549390A patent/JP3330930B2/en not_active Expired - Lifetime
- 1990-03-15 AU AU51301/90A patent/AU637354B2/en not_active Expired
- 1990-03-15 ZA ZA901973A patent/ZA901973B/en unknown
- 1990-03-15 KR KR1019900003438A patent/KR900014591A/en not_active Application Discontinuation
- 1990-03-15 IL IL9375490A patent/IL93754A/en not_active IP Right Cessation
- 1990-03-15 FI FI901296A patent/FI105103B/en active IP Right Grant
- 1990-03-15 IE IE93190A patent/IE74850B1/en not_active IP Right Cessation
- 1990-03-15 CA CA002012312A patent/CA2012312C/en not_active Expired - Lifetime
- 1990-03-16 RU SU5010032A patent/RU2136751C1/en active
-
1995
- 1995-06-30 HU HU95P/P00692P patent/HU211868A9/en unknown
-
1996
- 1996-09-03 GR GR960402292T patent/GR3020935T3/en unknown
Also Published As
Publication number | Publication date |
---|---|
DK0387775T3 (en) | 1996-11-04 |
JPH03130080A (en) | 1991-06-03 |
HUT54206A (en) | 1991-01-28 |
ATE140961T1 (en) | 1996-08-15 |
NO901213L (en) | 1990-09-17 |
IE900931L (en) | 1990-09-16 |
EP0387775A1 (en) | 1990-09-19 |
NO901213D0 (en) | 1990-03-15 |
IL93754A (en) | 1995-12-31 |
IL93754A0 (en) | 1990-12-23 |
AU5130190A (en) | 1990-11-01 |
ZA901973B (en) | 1991-11-27 |
PT93440A (en) | 1990-11-07 |
DD297838A5 (en) | 1992-01-23 |
FI105103B (en) | 2000-06-15 |
NZ232917A (en) | 1997-07-27 |
CA2012312C (en) | 2001-08-14 |
JP3330930B2 (en) | 2002-10-07 |
GR3020935T3 (en) | 1996-12-31 |
PT93440B (en) | 2001-07-31 |
ES2090049T3 (en) | 1996-10-16 |
CA2012312A1 (en) | 1990-09-16 |
RU2136751C1 (en) | 1999-09-10 |
EP0387775B1 (en) | 1996-07-31 |
HU215187B (en) | 1998-10-28 |
KR900014591A (en) | 1990-10-24 |
HU901584D0 (en) | 1990-06-28 |
FI901296A0 (en) | 1990-03-15 |
IE74850B1 (en) | 1997-08-13 |
DE59010432D1 (en) | 1996-09-05 |
HU211868A9 (en) | 1995-12-28 |
NO306623B1 (en) | 1999-11-29 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
AU637354B2 (en) | Genetic units for inhibiting the function of rna | |
Durand et al. | Functional complementation of major histocompatibility complex class II regulatory mutants by the purified X-box-binding protein RFX | |
AU4072089A (en) | Inhibition of htlv-iii by exogenous oligonucleotides | |
WO1996019568A3 (en) | Stabilized ribozyme analogs | |
ATE189264T1 (en) | CHIMERIC OLIGONUCLEOTIDE AND USE THEREOF FOR PRODUCING NUCLEIC ACID TRANSCRIPTS | |
AU6589094A (en) | 7-deazapurine modified oligonucleotides | |
UA42675C2 (en) | Method for gene activation of eucariotic cell line genom, which is transcriptionally latent in normal conditions, method for modification of gene expression characteristics for eucariotic cell line gene, differentiated eucariotic cell line (variants), dna-constract | |
EP0708829B8 (en) | A pharmaceutical composition comprising antisense-nucleic acid for prevention and/or treatment of neuronal injury, degeneration and cell death and for the treatment of neoplasms | |
AU7572096A (en) | Chimeric oligomers having an rna-cleavage activity | |
HUP0103465A2 (en) | Short oligonucleotides for the inhibition of vegf expression | |
WO1996001314A3 (en) | Ribozyme library, its production and use | |
DE69921609D1 (en) | RIBOCYMAL NUCLEIC ACID CUTTING THE CCR5 OR CXCR4 | |
Hatta et al. | Mechanisms of the inhibition of reverse transcription by unmodified and modified antisense oligonucleotides | |
AU6144294A (en) | Plant virus resistance conferring polyribozyme and resistant plants producing same | |
EP0592685A4 (en) | Plant resistant to two or more viruses and preparation thereof. | |
EP0691853A4 (en) | ANTISENSE OLIGOS WHICH INTERFERE WITH mRNA CAP ACTIVITY AND INHIBIT TRANSLATION | |
Goswami | Transcriptional induction of proto-oncogene fos by HSV-2 | |
Richardson et al. | Identification of upstream sequence components of rho-dependent transcription terminators. | |
AU3956100A (en) | Rna polymerase i transcription factor tif-ia | |
UA19318A (en) | Method for human mno2-dismutase obtaining | |
CA2103062A1 (en) | Oligo-2'-deoxynucleotides and their use as pharmaceutical agents with antiviral activity |