AU637354B2 - Genetic units for inhibiting the function of rna - Google Patents

Genetic units for inhibiting the function of rna

Info

Publication number
AU637354B2
AU637354B2 AU51301/90A AU5130190A AU637354B2 AU 637354 B2 AU637354 B2 AU 637354B2 AU 51301/90 A AU51301/90 A AU 51301/90A AU 5130190 A AU5130190 A AU 5130190A AU 637354 B2 AU637354 B2 AU 637354B2
Authority
AU
Australia
Prior art keywords
rna
units
inhibiting
genetic
unit
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
AU51301/90A
Other versions
AU5130190A (en
Inventor
Hartmut Beug
Max Birnstiel
Matthew Cotten
Harald Kandolf
Ernst Wagner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Boehringer Ingelheim International GmbH
Original Assignee
Boehringer Ingelheim International GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Ingelheim International GmbH filed Critical Boehringer Ingelheim International GmbH
Publication of AU5130190A publication Critical patent/AU5130190A/en
Application granted granted Critical
Publication of AU637354B2 publication Critical patent/AU637354B2/en
Anticipated expiration legal-status Critical
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C12N2310/111—Antisense spanning the whole gene, or a large part of it
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
    • C12N2310/121—Hammerhead

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Nitrogen Condensed Heterocyclic Rings (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The invention relates to a genetic unit, which is optionally in multicopy form, for the inhibition of RNA. The unit contains the transcription units necessary for transcription by polymerase III, and a DNA which codes for inhibiting RNA and which is arranged within the unit in such a way that the transcribed RNA is part of the polymerase III transcript. These units can be used to achieve increased stability of the inhibiting RNA, which can be in the form of ribozymes or antisense RNAs, while there is no reduction in the activity. The invention furthermore relates to a process for introducing the genetic units into the cell, to the use of these units and to pharmaceutical formulations containing them.
AU51301/90A 1989-03-16 1990-03-15 Genetic units for inhibiting the function of rna Expired AU637354B2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AT60989 1989-03-16
AT609/89 1989-03-16

Publications (2)

Publication Number Publication Date
AU5130190A AU5130190A (en) 1990-11-01
AU637354B2 true AU637354B2 (en) 1993-05-27

Family

ID=3495118

Family Applications (1)

Application Number Title Priority Date Filing Date
AU51301/90A Expired AU637354B2 (en) 1989-03-16 1990-03-15 Genetic units for inhibiting the function of rna

Country Status (20)

Country Link
EP (1) EP0387775B1 (en)
JP (1) JP3330930B2 (en)
KR (1) KR900014591A (en)
AT (1) ATE140961T1 (en)
AU (1) AU637354B2 (en)
CA (1) CA2012312C (en)
DD (1) DD297838A5 (en)
DE (1) DE59010432D1 (en)
DK (1) DK0387775T3 (en)
ES (1) ES2090049T3 (en)
FI (1) FI105103B (en)
GR (1) GR3020935T3 (en)
HU (2) HU215187B (en)
IE (1) IE74850B1 (en)
IL (1) IL93754A (en)
NO (1) NO306623B1 (en)
NZ (1) NZ232917A (en)
PT (1) PT93440B (en)
RU (1) RU2136751C1 (en)
ZA (1) ZA901973B (en)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH04505261A (en) * 1989-05-10 1992-09-17 スローン―ケツテリング・インステイテユート・フオー・キヤンサー・リサーチ Stably transformed eukaryotic cells containing transcribable foreign DNA under the control of a Pol III promoter
DE4091533T (en) * 1989-08-31 1992-01-30
JP2507895B2 (en) * 1989-12-19 1996-06-19 工業技術院長 New synthesis system of ribozyme
CA2084790C (en) * 1990-06-19 2002-04-09 Philip Anthony Jennings Endonucleases
US6008343A (en) * 1990-06-19 1999-12-28 Gene Shears Pty. Ltd. Nucleotide based endonucleases
DE552178T1 (en) 1990-10-12 1994-02-03 Max Planck Gesellschaft MODIFIED RIBOZYMS.
DE4104186A1 (en) * 1991-02-12 1992-08-13 Genentech Inc NEW COMPLEXES INCLUDING ENDOCYTOSIS IN HIGHER EUKARYOTIC CELLS, NUCLEIC ACID
FR2687411A1 (en) * 1992-02-13 1993-08-20 Nice Sophia Antipolis Universi Vector comprising a viral gene transcribed by RNA polymerase III, and process for the intercellular production of RNA
AU4259193A (en) * 1992-04-28 1993-11-29 Frank Andreas Harald Meyer Medicament for the gene-therapeutic treatment of human beings, animals and plants, especially to block virus multiplication and carcinogenes and process for producing the medicament
CA2135499A1 (en) * 1992-05-14 1993-11-25 James D. Thompson Method and reagent for inhibiting cancer development
US5989906A (en) * 1992-05-14 1999-11-23 Ribozyme Pharmaceuticals, Inc. Method and reagent for inhibiting P-glycoprotein (mdr-1-gene)
US5610052A (en) * 1992-08-26 1997-03-11 Ribozyme Pharmaceuticals Inc. Enzymatic RNA with activity to ras
US5827935A (en) * 1992-05-27 1998-10-27 City Of Hope Chimeric tRNAlys -ribozyme molecules
EP0596901B1 (en) * 1992-05-27 2000-08-09 City Of Hope CHIMERIC tRNA LYS -RIBOZYME MOLECULES
JPH08500971A (en) * 1992-06-29 1996-02-06 ジーン・シアーズ・ピーティーワイ・リミテッド Nucleic acids and their use for controlling viral pathogens
WO1994013798A1 (en) * 1992-12-16 1994-06-23 Bergmann Johanna E Agents for the prevention and treatment of human alzheimer's disease
DE69333216T2 (en) * 1993-07-06 2004-06-24 Université de Nice-Sophia Antipolis Vector, contains a virus gene transcribed in ARN polymerase III
US5837503A (en) * 1993-07-07 1998-11-17 Universite De Nice-Sophia-Antipolis Vector containing viral gene transcribed by RNA polymerase III and methods for use
US5817635A (en) * 1993-08-09 1998-10-06 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. Modified ribozymes
AU692208B2 (en) * 1994-01-24 1998-06-04 City Of Hope Inhibitors and target molecule co-localization
DE4424761C1 (en) * 1994-07-04 1995-06-08 Max Planck Gesellschaft Expression cassette for continuous, stable prodn. of ribozyme
EP0837933A4 (en) 1995-06-07 2003-05-21 Commw Scient Ind Res Org Optimized minizymes and miniribozymes and uses thereof
FR2755976B1 (en) 1996-11-15 1999-01-15 Idm Immuno Designed Molecules NOVEL COMPLEXES OF NUCLEIC ACIDS AND POLYMER SUBSTITUTED BY RESIDUES CAUSING THE DESTABILIZATION OF CELL MEMBRANES
US6057156A (en) 1997-01-31 2000-05-02 Robozyme Pharmaceuticals, Inc. Enzymatic nucleic acid treatment of diseases or conditions related to levels of epidermal growth factor receptors
WO1999020753A2 (en) * 1997-10-21 1999-04-29 Ribozyme Pharmaceuticals, Inc. Peptide bond formation using nucleic acid catalysts
US6506559B1 (en) 1997-12-23 2003-01-14 Carnegie Institute Of Washington Genetic inhibition by double-stranded RNA
EP1555317B1 (en) 1998-03-20 2011-09-28 Commonwealth Scientific And Industrial Research Organisation Synthetic genes and genetic constructs comprising the same
AUPP249298A0 (en) 1998-03-20 1998-04-23 Ag-Gene Australia Limited Synthetic genes and genetic constructs comprising same I
CA2361201A1 (en) 1999-01-28 2000-08-03 Medical College Of Georgia Research Institute, Inc. Composition and method for in vivo and in vitro attenuation of gene expression using double stranded rna
US6423885B1 (en) 1999-08-13 2002-07-23 Commonwealth Scientific And Industrial Research Organization (Csiro) Methods for obtaining modified phenotypes in plant cells
US20020132257A1 (en) 2001-01-31 2002-09-19 Tony Giordano Use of post-transcriptional gene silencing for identifying nucleic acid sequences that modulate the function of a cell
AUPR621501A0 (en) 2001-07-06 2001-08-02 Commonwealth Scientific And Industrial Research Organisation Delivery of ds rna

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4987071A (en) * 1986-12-03 1991-01-22 University Patents, Inc. RNA ribozyme polymerases, dephosphorylases, restriction endoribonucleases and methods

Also Published As

Publication number Publication date
DK0387775T3 (en) 1996-11-04
JPH03130080A (en) 1991-06-03
HUT54206A (en) 1991-01-28
ATE140961T1 (en) 1996-08-15
NO901213L (en) 1990-09-17
IE900931L (en) 1990-09-16
EP0387775A1 (en) 1990-09-19
NO901213D0 (en) 1990-03-15
IL93754A (en) 1995-12-31
IL93754A0 (en) 1990-12-23
AU5130190A (en) 1990-11-01
ZA901973B (en) 1991-11-27
PT93440A (en) 1990-11-07
DD297838A5 (en) 1992-01-23
FI105103B (en) 2000-06-15
NZ232917A (en) 1997-07-27
CA2012312C (en) 2001-08-14
JP3330930B2 (en) 2002-10-07
GR3020935T3 (en) 1996-12-31
PT93440B (en) 2001-07-31
ES2090049T3 (en) 1996-10-16
CA2012312A1 (en) 1990-09-16
RU2136751C1 (en) 1999-09-10
EP0387775B1 (en) 1996-07-31
HU215187B (en) 1998-10-28
KR900014591A (en) 1990-10-24
HU901584D0 (en) 1990-06-28
FI901296A0 (en) 1990-03-15
IE74850B1 (en) 1997-08-13
DE59010432D1 (en) 1996-09-05
HU211868A9 (en) 1995-12-28
NO306623B1 (en) 1999-11-29

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